KR102400706B1 - Method for increasing active ingredient of barley sprout using plasma treatment - Google Patents

Abstract

The present invention relates to the promotion of active ingredients of germinated barley sprouts through plasma treatment, and more particularly, a method for enhancing active ingredients of barley sprouts comprising treating the germinated barley shoots with plasma, and the promotion of active ingredients through the promotion method It relates to a germinated barley sprout, a food composition and a feed composition comprising the germinated barley sprout as an active ingredient.
Barley sprouts treated with plasma by the method of the present invention have improved growth, and the content of GABA, polyphenol, policosanol, saponarin, soluble sugars or amino acids is enhanced, so that excellent barley sprouts can be nurtured.

Description

Method for increasing active ingredient content of barley sprout according to plasma treatment {Method for increasing active ingredient of barley sprout using plasma treatment}

The present invention relates to the promotion of active ingredients of germinated barley sprouts through plasma treatment, and more particularly, a method for enhancing active ingredients of barley shoots comprising treating the germinated barley shoots with plasma, and the promotion of active ingredients through the promotion method It relates to a germinated barley sprout, a food composition and a feed composition comprising the germinated barley sprout as an active ingredient.

The sprout of a plant refers to the state with about 1 to 3 leaves that are 3 to 9 days old after the seed germinates, and refers to the state of young cotyledons before the actual leaves appear. Sprouts contain enzymes, amino acids, vitamins and minerals, as well as dietary fiber and functional physiologically active substances. It is an edible vegetable that is used by germinating from a seed and using all parts or cutting the stem part when the cotyledon is developed or before it. Sprout vegetables sold in the food market include broccoli, clover, radish, red radish, rapeseed, Chinese cabbage, cabbage, and red cabbage.

On the other hand, barley sprouts (young barley leaves) have long been known as a nutritionally excellent food source because of their high content of inorganic components such as calcium, magnesium and potassium as well as vitamin B1 and vitamin C content. , and functional secondary metabolites such as saponarin, lutonarin, and isovitexin, which have anticancer functions, are increasing, so that their industrial use is increasing as a material for health functional foods and pharmaceuticals.

In addition, the policosanol component contained in barley sprouts has the effect of raising the level of beneficial cholesterol (HDL; high density lipoprotein cholesterol) and lowering the level of harmful cholesterol (LDL; low density lipoprotein cholesterol), so it is preferred as a health functional food for middle-aged and elderly people. is becoming These barley sprouts were sown in open soil and used by cutting the sprout stems or washing the roots. About 70% of barley sprouts are chlorophyll, which has the effect of removing toxins from the body, relieving inflammation, preventing cell mutation, and healing wounds. In addition, barley sprouts contain anti-cancer substances, in addition to more than 10 vitamins, more than 20 amino acids, and many minerals and enzymes. The main ingredients contained in barley sprouts are 55 times more than milk, 18 times more potassium than spinach, and 11 times more calcium than milk. In addition, it is known that barley sprouts contain 5 times more ingredients to prevent anemia than spinach, and 3 times more vitamin C than spinach and 60 times more than apples.

In this regard, a technique (Korean Patent No. 10-2044686) that can increase the germination and growth efficiency of barley using mud and pine needles has been developed to promote the growth of the barley sprout and the active ingredient, but an appropriate plasma treatment step A method for enhancing the growth and active ingredient content of barley sprouts, including the, was first identified by the present inventors.

Under this background, the present inventors have intensively researched a method for enhancing the active ingredients of barley sprouts. As a result, when plasma is applied to germinated barley sprouts once, at 19.5w for 6 minutes, GABA, polyphenol, policosanol, saponarin, By confirming that the soluble sugar or amino acid increased the most, the present invention was completed.

One object of the present invention is to provide a method for enhancing the active ingredient of barley sprouts comprising the step of treating the germinated barley sprouts with plasma.

Another object of the present invention is to provide germinated barley sprouts with enhanced active ingredients through the above method.

Another object of the present invention is to provide a food composition comprising the germinated barley sprout as an active ingredient.

Another object of the present invention is to provide a feed composition comprising the germinated barley sprout as an active ingredient.

One embodiment of the present invention for achieving the above object provides a method for enhancing the active ingredient of barley sprouts comprising the step of treating the germinated barley sprouts with plasma.

More specifically, the active ingredient is GABA, polyphenol, policosanol, saponarin, soluble sugar or amino acid, and the plasma is treated with 18w to 20w for 4 to 10 minutes, and one treatment to germinated barley sprouts. may be, but is not limited thereto.

As used herein, the term “germinated barley sprout” may be a sprout of germinated barley.

The term “germination” refers to a phenomenon in which shoots generated in seeds, spores, pollen, branches or roots of plants start to develop or grow, specifically, sprouting from seeds. Seeds or spores start activity and a new plant comes out by tearing the skin.

The above term, “barley” is a crop belonging to the Grape family, and is also called daemaek (大麥) in Chinese characters. The scientific name is Hordeum vulgare L. As a monthly herb, it is one of the oldest food crops. It was cultivated in the wild 7,000 years ago and is widely distributed in various climatic conditions around the world. The height of barley is about 50~100cm depending on the variety, and the stem between the nodes is empty inside the internodes and gets longer towards the top, and the first internode under the ears is the longest and thinnest. Leaves are alternate phyllotaxis, lanceolate, 5-20 cm long, and flowers 10-15 mm long. In Korea, flowers bloom from the end of April to early May, and then mature in 30 to 40 days. The fruit is a young fruit.

The above term, “sprout” is the first leaf and rod sprouting from the seed (root, stem, branch tip, etc.) of a plant. As a growth point, it is also called infant (幼芽), new branch, and young eye (plumule). It refers to the whole body of the stem and leaves, excluding the root. The sporophyte of vascular plants consists of leaves, stems, and roots, meaning that stems and leaves are formed from the same meristem.

In the present invention, the germinated barley sprout can be used as it is without damaging the original shape of barley sprout, or after performing a pretreatment process in consideration of the process speed and process (manufacturing) efficiency intended by those skilled in the art, the pretreatment The process may include, for example, conventional screening, washing, cutting, powdering, drying, and the like steps.

As used herein, the term “GABA (γ-aminobutyric acid)” is a kind of non-proteinaceous amino acid distributed in nature. It is an essential component of the central nervous system, such as the brain or spinal cord, and is known as a major inhibitory neurotransmitter. have. GABA is involved in many physiological regulation, and in the case of animals, it activates blood flow to the brain and increases oxygen supply to the brain, thereby promoting the metabolic function of brain cells. It is effective in preventing adult diseases, and has a nerve stabilizing function for pain relief, depression, and anxiety. It has been reported that modern people who suffer from stress, especially examinees and alcoholics, have low brain and blood GABA levels, and an extreme lack of GABA levels in the body causes seizures, convulsions, and epilepsy. Due to such physiological action, GABA is used as an intravenous injection to improve cerebral blood flow and enhance brain metabolism in stroke, head trauma, and cerebral artery sequelae, and is also sold as an oral prescription drug.

As used herein, the term “polyphenol” is one of the anti-oxidative substances that change free radicals in our body into harmless substances, and there are thousands of types of polyphenols. Among them, relatively widely known are catechins in green tea, resveratrol in wine, and quercetin in apples and onions. Many flavonoids in fruits and isoflavones in soybeans are also polyphenols. It is reported that polyphenols reduce the risk of various diseases due to their high antioxidant ability to protect DNA or cellular proteins and enzymes that are damaged by exposure to free radicals. In addition, polyphenols are known to have anti-cancer properties and prevent heart disease.

As used herein, the term “policosanol” is a natural fatty alcohol mixture extracted from plants, a natural fatty alcohol mixture extracted from plant wax, and two or more alcohols are linked together like a chain. Policosanol is contained in various plants such as rice bran and green tea leaves, including sugar cane. When 100 tons of sugar cane are processed, only about 2.5 kg is extracted. Policosanol, which is extracted from sugar cane, was first made in Cuba, and in clinical trials, it is known that long-term use of policosanol lowers cholesterol levels and helps people with high blood pressure.

As used herein, the term “saponarin” refers to a type of flavone glucoside, derived from barley sprouts.

As used herein, the term “soluble sugar” is water-soluble and used as a carbon substrate by microorganisms in fermentation processes to produce biofuels, biochemicals, or also to be used as sugars in any food, pharmaceutical or other application. all possible polysaccharides and monosaccharides and mixtures thereof.

As used herein, the term "amino acid" is a compound having both a basic amino group (-NH2) and an acidic carboxyl group (-COOH), most of which are colorless crystals and are readily soluble in water. Amino acids are representative amphoteric electrolytes and are an important component of proteins. When proteins are hydrolyzed with acids or enzymes (pepsin, trypsin, erepsin), various types of amino acids are formed.

In the present invention, the enhanced amino acid may be serine, aspartic acid, threonine, alanine, cysteine, lysine, tyrosine, methionine, valine, isoleucine, leucine or phenylalanine, but is not limited thereto.

As used herein, the term “plasma” refers to an ionized gas satisfying Debye shielding. It is regarded as another state along with the three basic states of matter, gas, liquid, and solid, and is expressed as the fourth state. In the plasma, a neutral gas is phase-transformed to the plasma by an external voltage, and electrons and cations may be generated by excitation and ionization of the neutral gas, and radicals in which molecular gas is excited may exist.

In one embodiment of the present invention, it was confirmed that when the plasma was treated with 19.5w for 6 minutes once in germinated barley sprouts, GABA, polyphenol, policosanol, saponarin, soluble sugar or amino acid increased the most, It was confirmed that the method of enhancing the active ingredient of the present invention was excellent.

Another aspect of the present invention for achieving the above object is to provide germinated barley sprouts with an enhanced active ingredient through the method.

The term, “germinated barley sprout” is the same as described above.

Another aspect of the present invention for achieving the above object provides a food composition comprising the germinated barley as an active ingredient.

The term, “germinated barley sprout” is the same as described above.

As used herein, the term “food” refers to meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages , vitamin complexes, health functional foods, etc. include all foods in the usual sense, and as long as it can contain gold or yellow white of the present invention, it is not limited thereto.

As used herein, the term “health functional food” refers to food manufactured and processed using raw materials or ingredients useful for the human body in accordance with Act No. 6727 of the Health Functional Food Act, and 'functionality' refers to the structure of the human body. And it means to obtain a useful effect for health use, such as regulating nutrients for function or physiological action. On the other hand, health food means food that has an active health maintenance or promotion effect compared to general food, and health supplement means food for the purpose of health supplementation. In some cases, the terms health functional food, health food and health supplement food can be mixed.

The food of the present invention can be prepared by a method commonly used in the art, and at the time of manufacture, it can be prepared by adding raw materials and components commonly added in the art. Specifically, the food composition may further include a physiologically acceptable carrier, the type of carrier is not particularly limited and any carrier commonly used in the art may be used. In addition, the food composition contains food additives such as preservatives, disinfectants, antioxidants, colorants, coloring agents, bleaching agents, seasonings, sweeteners, flavoring agents, expanding agents, strengthening agents, emulsifying agents, thickeners, filming agents, gum base agents, foam inhibitors, solvents, and improving agents. may include The additive may be selected according to the type of food and used in an appropriate amount.

Another aspect of the present invention for achieving the above object provides a feed composition comprising the germinated barley sprout as an active ingredient.

The term, “germinated barley sprout” is the same as described above.

As used herein, the term "feed" means any natural or artificial diet, meal, etc., or a component of said meal, intended for or suitable for being eaten, consumed, and digested by an animal.

The type of feed is not particularly limited, and feed commonly used in the art may be used. Non-limiting examples of the feed include plant feeds such as grains, root fruits, food processing by-products, algae, fibers, pharmaceutical by-products, oils and fats, starches, gourds or grain by-products; and animal feeds such as proteins, inorganic materials, oils and fats, minerals, oils and fats, single cell proteins, zooplankton, or food. These may be used alone or in mixture of two or more.

Barley sprouts treated with plasma by the method of the present invention are enhanced in growth and the content of GABA, polyphenol, policosanol, saponarin, soluble sugar or amino acid is enhanced, so that excellent barley sprouts can be grown.

1 is a graph showing the growth when plasma is directly treated to seeds before germination.
2 is a graph showing the growth when plasma is directly treated on germinated barley sprouts.
3 is a graph showing the live weight when plasma is directly treated on germinated barley sprouts.
4 is a graph showing the GABA content of barley sprouts according to plasma treatment.
5 is a graph showing the GAD activity of barley sprouts according to plasma treatment.
6 is a graph showing the content of polyphenols in barley sprouts according to plasma treatment.
7 is a graph showing the content of saponarin in barley sprouts according to plasma treatment.
8 is a graph showing the content of policosanol in barley sprouts according to plasma treatment.

Hereinafter, the present invention will be described in more detail through the following examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples only.

Example 1: Plasma Treatment

Plasma treatment was performed at normal pressure and room temperature using Surface Dielectric Barrier Discharge (SDBD), and the source of the plasma was 19.5W.

More specifically, two pieces of filter paper were laid on a Petri dish, 10 mL of distilled water was poured, and then, the new wheat barley seeds in the early stage of germination were spread evenly and treated for 6 minutes. While culturing in an incubator at 15°C for 9 days, plasma treatment was performed for 6 minutes each on days 1 (T1-9G), 1 and 2 (T2-9G), and 1 to 3 (T3-9G), and growth was continued on the 9th day. After irradiation, freeze-drying was used for component analysis. In addition, for comparison, plasma treatment was performed on the seeds before germination (T0-9G), and after germination for 9 days, comparison was made.

Example 2: Growth comparison

The growth of seeds T1-9G, T2-9G, and T3-9G treated with plasma at the early stage of germination cultivated through the method of Example 1 and seeds T0-9G treated with plasma treatment before germination were compared.

As a result, as can be seen in FIGS. 1 and 2 , when plasma treatment was treated after germination rather than before germination, it was confirmed that the overall length of barley was increased. It was confirmed that a significant effect appeared compared to the groups.

In addition, as can be seen in FIG. 3 , the most remarkable effect was shown in the group treated with plasma for 6 minutes only on the first day, which was confirmed to be increased by 37.6% compared to the group not treated with plasma.

Through this, it was confirmed that plasma treatment after germination was more effective for the development of seeds than plasma treatment before germination, and it was confirmed that the condition of treatment for 6 minutes on the first day was superior to other conditions.

Example 3: GABA content analysis

The GABA content of seeds T1-9G, T2-9G, and T3-9G treated with plasma at the early stage of germination cultivated through the method of Example 1 and seeds T0-9G treated with plasma treatment before germination were analyzed.

More specifically, GABA and glutamic acid contents were measured using an AccQ·Tag UPLC (Waters, Milford, MA) analysis system. The sample was filtered using a membrane filter, and the filtered sample was derivatized using the AccQ-Tag method for UPLC analysis. Water Acquity was used for the UPLC analysis system, the column was AccQ Tag Ultra Amino Acid Analysis column (3.9 × 150mm; waters), the column temperature was 49℃, the sample temperature was 20℃, and the detector was a FLR Detector (waters) with a wavelength Silver EX. 266 nm, EM. 473 nm. Mobile phase A is 100% AccQ Tag ultra UPLC amino acid analysis eluent A, B is 10% AccQ Tag ulta UPLC amino acid analysis eluent B, C is 100% distilled water, D is 100% AccQ Tag UPLC amino acid analysis eluent B was subjected to gradient elution, the flow rate was 0.7 ml/min, the injection volume was 1 μL, and each sample was measured in triplicate. Chromatogram data were analyzed using Empower personal software (waters).

As a result, as shown in FIG. 4 , it was confirmed that the GABA content was increased in germinated barley sprouts treated with plasma.

In addition, as can be seen in FIG. 5 , it was confirmed that the GAD activity was increased in the germinated shoots treated with plasma.

Through this, it was confirmed that GABA content and GAD activity were increased when plasma treatment was performed on germinated barley sprouts.

Example 4: Analysis of polyphenol and saponarin content

Polyphenol content of seeds T1-9G, T2-9G, and T3-9G treated with plasma in the early germination period cultivated through the method of Example 1 and seeds T0-9G treated with plasma treatment before germination were analyzed.

Specifically, the polyphenol content analysis was analyzed by the Folin-Ciocalteu method. More specifically, 1 g of each sample was sonicated in 10 mL distilled water at 30° C. for 2 hours, centrifuged at 15,000 x g for 10 minutes, and the supernatant was filtered through a 0.2 μm membrane. Thereafter, analysis was performed using an HPLC system equipped with ELSD (Alltech ELSD 2000) and a Grace Prevail Carbohydrate ES column (5 μm, 250 × 4.6 mm i.d.), 10 μL of the sample was injected at a column temperature of 30° C., and acetonitrile The solution (75% v/v) was the mobile phase and the flow was set to 1 mL/min. Nitrogen (48 mm Hg) was sprayed on the eluate coming out of the column, and the evaporation condition of the chromatography eluent was 90°C.

As a result, as can be seen in FIG. 6 , it was confirmed that the polyphenol content was the highest in the group treated with plasma for 6 minutes only on the first day.

Through this, it was confirmed that the condition of treating the plasma for 6 minutes on the first day of germination had an excellent effect on increasing the content of the active ingredient than other conditions.

Example 5: Saponarin content analysis

Polyphenol content of seeds T1-9G, T2-9G, and T3-9G treated with plasma in the early germination period cultivated through the method of Example 1 and seeds T0-9G treated with plasma treatment before germination were analyzed.

More specifically, the analysis of saponarin was analyzed using HPLC (Dionex Ultimate 3000, Thermo), and the column was waters ACQUTY BEH C18 1.7㎛, 2.1×100mm, UV detector (325nm), column oven temperature 35℃, The experiment was conducted under the conditions of sampler temperature 15℃, flow rate 0.5ml/min, injection volume 2μl. For the analysis, 0.1% Trifluoroacetic acid in winter was used for phase A and Acetonitrile was used for phase B as the solvent conditions for the mobile phase.

As a result, as can be seen in Table 1 and FIG. 7 , it was confirmed that the saponarin content was the highest in the group treated with plasma for 6 minutes only on the first day.

Saponarin content (mg/100g) 0 min 6 min 12 min 18 min 24 min 9 days 174.18 146.58 140.76 140.88 175.29

Through this, it was confirmed that the condition of treating the plasma for 6 minutes on the first day of germination had an excellent effect on increasing the content of the active ingredient than other conditions.

Example 6: Policosanol content analysis

Polyphenol content of seeds T1-9G, T2-9G, and T3-9G treated with plasma in the early germination period cultivated through the method of Example 1 and seeds T0-9G treated with plasma treatment before germination were analyzed.

More specifically, after sonication of 1 g of sample with 20 mL of Hexane at room temperature for 10 min. The extract was stirred at 200 rpm for 24 hours, centrifuged at 7000 rpm and 10° C. for 10 minutes, filtered through a 0.45 μm filter, and concentrated by injection into a concentrator. After dissolving the concentrate in 2mL CHCl ₃ , mix 500 μl of the concentrate, 100 μl of 1000 ppm Triacontanol, and 400 μl N-methyl-N-trifluoroacetamide to derivatize it at 60° C. for 20 minutes. After mixing 900 μl CHCl3 with 100 μl of the derivative solution, Filtration to 0.20 μm was analyzed by GC/MS. GC/MS is an Agilent 7890B GC system, Agilent 5977A MSD and Autosampler (Agilent 7693). In this case, the analysis conditions were HP-5ms, Agilent 190915-431, and Helup gas was used as the mobile phase. Heater of GC/MS was 250℃, flow was 16.2 mL/min, and septum purge flow was 3 mL/min. Standard materials were analyzed using Eicosanol, Heneicosanol, Docosanol, Tricosanol, Tetracosanol, Hexacosanol, Heptacosanol, Octacosanol, and Triacontanol.

As a result, as can be seen in FIG. 8 , it was confirmed that the content of policosanol was the highest in the group treated with plasma for 6 minutes only on the first day.

Through this, it was confirmed that the condition of treating the plasma for 6 minutes on the first day of germination had an excellent effect on increasing the content of the active ingredient than other conditions.

Example 7: Analysis of Soluble Sugar Content

Polyphenol content of seeds T1-9G, T2-9G, and T3-9G treated with plasma in the early germination period cultivated through the method of Example 1 and seeds T0-9G treated with plasma treatment before germination were analyzed.

Specifically, soluble sugars were analyzed by the Hernandez method using the HPLC-ELSD (Waters) system. More specifically, fructose, glucose and circus standards were purchased from Merck (La Coruna, Spain), and maltose standards were purchased from Sigma (Madrid, Spain). Samples were sonicated in 10 mL distilled water of 1 g each at 30° C. for 2 hours, centrifuged at 15,000 x g conditions for 10 minutes, and the supernatant was filtered through a 0.2 μm membrane. Thereafter, analysis was performed using an HPLC system equipped with ELSD (Alltech ELSD 2000) and a Grace Prevail Carbohydrate ES column (5 μm, 250 × 4.6 mm i.d.), 10 μL of the sample was injected at a column temperature of 30° C., and acetonitrile The solution (75% v/v) was the mobile phase and the flow was set to 1 mL/min. Nitrogen (48 mm Hg) was sprayed on the eluate coming out of the column, and the evaporation condition of the chromatography eluent was 90°C.

As a result, as can be seen in Table 2, it was confirmed that the content of soluble sugar was the highest in the group treated with plasma for 6 minutes only on the first day.

Through this, it was confirmed that the condition of treating the plasma for 6 minutes on the first day of germination had an excellent effect on increasing the content of the active ingredient than other conditions.

Example 8: Amino Acid Content Analysis

Polyphenol content of seeds T1-9G, T2-9G, and T3-9G treated with plasma in the early germination period cultivated through the method of Example 1 and seeds T0-9G treated with plasma treatment before germination were analyzed. The amino acid content analysis method is the same as the GABA content analysis method of Example 3.

As a result, as can be seen in Table 3, it was confirmed that the content of a number of amino acids increased the most in the group treated with plasma for 6 minutes only on the first day.

Through this, it was confirmed that the condition of treating the plasma for 6 minutes on the first day of germination had an excellent effect on increasing the content of active ingredients than other conditions.

From the above description, those skilled in the art to which the present invention pertains will understand that the present invention may be embodied in other specific forms without changing the technical spirit or essential characteristics thereof. In this regard, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. The scope of the present invention should be construed as being included in the scope of the present invention, rather than the above detailed description, all changes or modifications derived from the meaning and scope of the claims to be described later and their equivalents.

Claims (9)

A method for promoting active ingredients of barley sprouts, comprising the step of treating the germinated barley sprouts with plasma.
The method of claim 1, wherein the active ingredient is GABA, polyphenol, policosanol, saponarin, soluble sugar or amino acid.
The method of claim 1, wherein the plasma is treated for 4 to 10 minutes.
The method of claim 1, wherein the plasma is treated with 18w to 20w.
The method of claim 1, wherein the plasma is treated once in germinated barley sprouts.
The method of claim 2, wherein the amino acids are serine, aspartic acid, threonine, alanine, cysteine, lysine, tyrosine, methionine, valine, isoleucine, leucine or phenylalanine.
According to any one of claims 1 to 6, through the promotion method of any one of claims, germinated barley sprouts with an enhanced active ingredient.
A food composition comprising the germinated barley sprout of claim 7 as an active ingredient.
A feed composition comprising the germinated barley sprout of claim 7 as an active ingredient.

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