KR20170065452A - Lotus tea beverage and manufacturing method thereof - Google Patents
Lotus tea beverage and manufacturing method thereof Download PDFInfo
- Publication number
- KR20170065452A KR20170065452A KR1020160162625A KR20160162625A KR20170065452A KR 20170065452 A KR20170065452 A KR 20170065452A KR 1020160162625 A KR1020160162625 A KR 1020160162625A KR 20160162625 A KR20160162625 A KR 20160162625A KR 20170065452 A KR20170065452 A KR 20170065452A
- Authority
- KR
- South Korea
- Prior art keywords
- extract
- lotus root
- lotus
- root
- soft
- Prior art date
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
- A23L2/04—Extraction of juices
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/06—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/065—Microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/121—Brevis
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/31—Leuconostoc
- A23V2400/323—Paramesenteroides
-
- A23Y2220/13—
-
- A23Y2260/41—
Abstract
본 발명은 연 음료 및 그 제조방법에 관한 것으로 연잎, 연자방, 연근, 연줄기 및 연자를 당 추출, 효소처리 또는 유산균 발효법을 이용하여 연 음료를 제조한 다음 상기 제조된 연 음료의 이화학적 특성 및 생리활성 측정하여 기능성 효과를 확인하고, 관능평가를 실시함으로써 기능적, 품질적인 측면에서 우수한 연 음료를 제공할 수 있는 효과가 있을 뿐 아니라 풍미가 우수한 연 음료를 제공할 수 있으므로 연의 이용도를 극대화 할 수 있는 뛰어난 발명이다. The present invention relates to a soft drink and a method for producing the soft drink, and more particularly, to a soft drink and a process for producing the soft drink, which comprises preparing a soft drink by using sugar extraction, enzyme treatment or lactic fermentation, And physiological activity to confirm the functional effect and to perform the sensory evaluation, it is possible to provide an excellent soft drink in terms of function and quality, and it is also possible to provide a soft drink having excellent flavor, It is an excellent invention to do.
Description
본 발명은 연 음료 및 그 제조방법에 관한 것으로 더욱 상세하게는 연잎, 연자방, 연근, 연줄기 및 연자를 덖음, 당추출, 효소처리 또는 유산균 발효법을 이용하여 제조하는 연 음료 및 그 제조방법에 관한 것이다.The present invention relates to a soft drink and a method of manufacturing the same, and more particularly to a soft drink and a method for manufacturing the soft drink, which are manufactured by using a soft drink, a soft drink, a lotus root, .
연(Lotus, Nelumbo nucifera Gaertner)은 수련과에 속하는 여러해살이 수생식물 중 부엽식물에 속하는 쌍떡잎식물로 주로 연못에서 자라고 논밭에서 재배되기도 한다. 연의 종류는 북아메리카 원산인 황화연(黃化蓮)과 동양 원산인 연(蓮) 두 종으로 구분되며, 중국의 품종에는 지방 백화종(地方白化種)과 비중종(備中種) 있는데 우리나라에는 비중종이 널리 보급되어 있으며 가장 많이 재배되고 있다. 우리나라의 연은 인도에서 유래한 종이며, 한국 종은 만생종으로 꽃은 담홍색이고, 뿌리는 타원형으로 4 ~ 5월에 뿌리를 심어 9월초에서 이듬해 5월 상순까지 수시로 수확한다(Borsch T, BarthlottW. 1994. Classification and distribution ofthe genus Nelumboadans(Nelumbonaccae), BeitrBiolPflazen 68 : 421-450). 연은 예로부터 차와 술로 많이 이용하였다. 꽃은 연화차를 만들고, 잎은 하엽차, 뿌리는 연근차를 만들어 음용했고, 연엽주라고 하여 연꽃잎을 넣어 향기로운 술을 만들기도 하였다(Kim YS.Chun SS,Jung ST and Kim RY.2002.Effects oflotus root powderon the quality ofdough.Korean J.Soc.Food Cookery Sci.18(6): 573-578). 동양의학에서 연은 매우 유용한 한약재로 사용하고 있는데, 열매에서부터 잎, 꽃, 연밥, 연의 암술, 뿌리에 이르기까지 연의 대부분을 활용하고 있다.Lotus (Nelumbo nucifera Gaertner) is a perennial aquatic plant belonging to the water lily family. It is a dicotyledonous plant that grows mainly in a pond and is cultivated in a field. The species of Yan is divided into two kinds of North China, Huanghua (Lotus) and Oriental (Lotus), and Chinese varieties have local white and white species. And it is the most cultivated. Korea is a species derived from India. Korean species are native to Korea, flowers are light pink, and roots are elliptical. They are rooted in April to May, and harvested from early September to early May of the following year (Borsch T, Barthlott W. 1994. Classification and distribution of the genus Nelumboadans (Nelumbonaccae), BeitrBiolPflazen 68: 421-450). Kite has been widely used for tea and liquor since ancient times. The flowers made soft tea, the leaves were low tea leaves, the roots were roots made of tea, and the petals were added to the leaves to make fragrant sake (Kim YS.Chun SS, Jung ST and Kim RY.2002.Effects oflotus root powderon the quality ofdough. Korean J. Soc. Good Cookery Sci. 18 (6): 573-578). In oriental medicine, kite is used as a very useful medicinal herb. It uses most of the kite from fruit to leaves, flowers, rice, kite, root.
연잎은 하엽(荷葉)이라 하며, 맛이 쓰고 성질은 유하다 하여 예로부터 출혈성 위궤양이나 위염, 치질, 출혈, 설사, 두통과 어지럼증, 토혈, 산후 어혈치료, 야뇨증, 해독작용으로 민간 치료제로 사용하여 왔다(Quality characteristics of fish paste containing lotus leaf powder. Korean J Food Cookery Sci 23(6):947?953). 연잎에서 분리된 성분으로는 nelumboside, nuciferine, coclaurine 등의 알카로이드, galic acid, methl gallate 등의 방향성분 및 quercetin, isoquercetin, rutin, kaempferol 등의 플라보노이드류가 있다(Choi, S. J., Kim, S. Y., Jeon, Y. J., Koo, C. S., Ha, B.J., & Chae HJ. (2011). Stability evaluation of the cosmetics containing lotus leaf extract. Korean Society of Biotechnolory & Bioengineering, 26, 83-86).Yeonpyeon is called as a lower leaf. It is used as a cure medicine because of its taste and its nature. It is used as a treatment for hemorrhagic gastric ulcer, gastritis, hemorrhoids, bleeding, diarrhea, headache and dizziness, hematemesis, postpartum hemorrhage, (Korean J Food Cookery Sci 23 (6): 947-953). Some of the components isolated from the leaves are aromatic compounds such as nelumboside, nuciferine, coclaurine, galic acid and methl gallate, and flavonoids such as quercetin, isoquercetin, rutin and kaempferol (Choi, SJ, Kim, YJ, Koo, CS, Ha, BJ, & Chae HJ (2011). Stability evaluation of the cosmetics containing lotus leaf extract. Korean Society of Biotechnology & Bioengineering, 26, 83-86).
연근은 연우(蓮藕)라고 하며 맛은 달고 따뜻한 성질을 지니고 있으며, 진흙 속을 가로 기는 땅속줄기는 마디가 있는 흰색으로, 연근에서 잎이 되는 줄기와 꽃이 피는 줄기가 생긴다. 연의 땅속줄기인 연근에는 빈 구멍이 있고, 조직이 단단하며 씹히는 맛이 산뜻하여 날로 씹어 먹거나 즙을 내서 먹기도 하며, 요리나 약재로 다양하게 활용되는 건강식품이다. 연근의 일반성분은 녹말 등의 탄수화물이 약 14%이고, 아스파라긴, 아르기닌, 타이로신, 레시친 등과 함께 비타민 C가 20㎎% 정도 함유되어 있다. 특히 무기질과 식이섬유 등이 풍부해 피부건강과 콜레스테롤 저하에 도움을 주며 다른 뿌리식물에 비해 항산화작용과 항암 작용을 하는 비타민 C가 다량 함유되어 있고, 항암성분으로 알려진 폴리페놀도 함유하고 있다(Han S.J. et al., Study on the chemical composition in bamboo shoot, lotus root and burdock: free sugar, fatty acid, amino acid and dietary fiber contents, Korean J. Soc. Food Sci., 9, 82-87, 1993). 연근의 효능은 탄닌, 철분이 풍부하여 지혈과 소염작용이 알려져 있으며, 반으로 가르면 나오는 액체인 '뮤신'은 당단백질 성분으로 위산의 분비를 조절하여 각종 위 질환을 예방하고, 손상된 위벽을 빠르게 회복시켜준다. 연근을 꾸준히 섭취하면 모세혈관 혈행 개선과 말초 혈액순환 촉진으로 피부 신진대사가 활발해져 여드름이나 기미 등 피부미용에 도움을 주기 때문에 건강기능식품, 화장품 및 의약품 소재로서 널리 사용되고 있다.Lotus root is called 蓮藕 (蓮藕), and its taste is warm and possesses a warm temperament. In the mud, the subterranean stem is white with nodule, and there are stem and flower stalks in the lotus root. Lotus root, which is a subterranean root of the stalk, has a hollow hole, and it is a healthy food that is hard and has a chewy taste and is chewy, juiced and eaten. The general component of the lotus root is about 14% of carbohydrates such as starch, and contains about 20 mg% of vitamin C together with asparagine, arginine, tyrosine, lecithin and the like. It is rich in minerals and dietary fiber, which helps skin health and cholesterol lowering. It also contains a large amount of vitamin C, which is antioxidant and anti-cancerous, compared with other root plants. It also contains polyphenols SJ. Food Sci., 9, 82-87,1993). In this study, we investigated the effect of bamboo shoots on the production of bamboo shoots, lotus root and burdock, free sugars, fatty acid, amino acid and dietary fiber contents. Mucin is a glycoprotein component that regulates the secretion of gastric acid to prevent various gastric diseases and to rapidly repair damaged gastric wall. I will. It is widely used as health functional food, cosmetics and medicine materials because it helps to improve skin capillary blood circulation and promotes peripheral blood circulation, thereby promoting skin metabolism and helping skin beauty such as acne and spots.
연자는 연밥, 연육, 연실, 상련, 종자, 연자육, 연씨, 연애 등 여러가지 이름이 있다. 연자는 전분, 탄수화물, 단백질, 지방 등의 주요 영양소와 비타민 C, 비타민 B1, 비타민 B2, 철분, 칼슘, 인, 나이아신, 아스파라긴산, 구리, 망간, 넬룸빈, 라피노스 등의 미량 성분이 고루 들어 있어 우수 영양식품으로 맛이 달고 성질이 순하여 비장, 신장, 심장과 경락에 작용하는 약재로 사용하고 있다. 연자에는 nuciferine, oxoushinunine, N-methylasimilobine, lirindine, higena, quercetin, rutin, hyperin 등이 보고되었으며(Lee, B. G. & Byun, K. I. (2008). Rheological properties of white pan bread dough prepared with lotus(Nelumbo nucifera) seeds powder. Korean Journal of Food Preservation, 15(6), 852-858), 연자의 기능성에 대해서는 항우울 효과, 항산화 기능, 세포독성 보호효과, 심장질환 치료효과, 항경련 효과 등의 연구가 보고되었다.Consists of various names such as rice gruel, rice cultivar, rice field, rice paddy, seed, prawn, Yan, romance. It is composed of main nutrients such as starch, carbohydrate, protein and fat and trace elements such as vitamin C, vitamin B1, vitamin B2, iron, calcium, phosphorus, niacin, asparaginic acid, copper, manganese, nelumbin and raffinose. It is a nutritional food that is used as a medicinal substance that acts on the spleen, kidneys, heart and meridians. In this study, we investigated the effects of nutrients such as nuciferin, oxoushinunine, N-methylasimilobine, lirindine, higena, quercetin, rutin and hyperin (Lee, BG and Byun, KI The effects of antidepressant, antioxidant, cytotoxic, cardioprotective, and anticonvulsant effects on soft tissue function were reported in the Journal of Food Preservation, 15 (6), 852-858.
일반적인 효소는 효모, 세균 또는 일부 고등식물 등에서 분리되며, 가장 일반적인 적용으로 식물조직의 세포벽이나 세포 사이를 연결해주는 펙틴을 분해하는 pectinase를 이용하여, 과실이나 야채의 조직을 연화시키고 당을 생산하거나 과일 가공 시에 주스의 생산 수율을 높이는 데 주로 사용되고 있다. 원료로부터 물질을 효과적으로 추출해내기 위해 효소적 가수분해방법을 적용하고 있으며, 이러한 효소들은 multiple enzyme으로 cell wall에 있는 섬유질이나 당단백질 혹은 특이효소에 의한 고분자 물질 분해시키는 작용 등을 이용하여 활성 물질이 원활히 추출될 수 있도록 유도해 주는데 유효한 추출법으로 활용되고 있는 실정이다. Common enzymes are isolated from yeast, bacteria, or some higher plants. The most common application is to use pectinase, which degrades pectin to connect cell walls or cells between plant tissues, to soften fruit or vegetable tissues, It is mainly used to increase the production yield of juice during processing. Enzymatic hydrolysis methods are applied to efficiently extract substances from raw materials. These enzymes are multiple enzymes, and by using the action of decomposing polymer substances by fiber, glycoprotein or specific enzyme in cell wall, This method is used as an effective extraction method to induce the extraction.
유산균(lactic acid bacteria)은 사람이나 동물의 장(腸)과 발효식품 등에서도 쉽게 발견되는 안전한 미생물로(Orrhge, K. et al., 2000, Bifidobacteria and lactobacilli in human health, Drugs Exptl. Clin. Res., 26, 95-111) 장내 상피세포에 부착하여 기생하면서 장내 균총의 성질을 개선시켜 장내 균총의 안정화, 유해세균의 정착 억제에 따른 부패산물 생성 감소 및 질병 예방, 면역 활성화 작용, 항암작용, 콜레스테롤 저하 등 인체에 많은 도움을 주는 것으로 알려져 있다. 유산균이 여러 부패성 미생물 및 병원성 미생물에 대하여 생육억제 작용을 갖는 것은 몇 가지 대사적인 특성 때문인데 유산균은 대사산물로서 항균활성 인자인 organic acid, hydrogen peroxide, reuterin, diacetyl, acetaldehyde, bacteriocin 등을 생산하기 때문이다(Fuller, K., 1989, Probiotics in man and animals, J. Appl. Bacteriol., 66, 365-378).Lactic acid bacteria are safe microorganisms that are easily found in intestines and fermented foods of humans and animals (Orrhge, K. et al., 2000, Bifidobacteria and lactobacilli in human health, Drugs Exptl. ., 26, 95-111). It has been shown to improve the properties of intestinal microflora by parasitic attachment to intestinal epithelial cells, thereby reducing intestinal microflora stability, decreasing the production of decay products due to inhibition of harmful bacteria and preventing disease, Cholesterol lowering is known to help the body a lot. Lactic acid bacteria have several inhibitory effects on various pathogenic microorganisms and pathogenic microorganisms due to their several metabolic characteristics. The lactic acid bacteria produce the antimicrobial activity factors such as organic acid, hydrogen peroxide, reuterin, diacetyl, acetaldehyde and bacteriocin (Fuller, K., 1989, Probiotics in man and animals, J. Appl. Bacteriol., 66, 365-378).
연 음료에 관한 선행기술로 연자를 이용한 음료 및 제조방법이 대한민국 공개특허 제 10-2010-0008929호가 공개된 바 있고, 연씨, 연잎, 연씨집, 연꽃을 덖어 정제수로 추출한 다음 올리고당, 복합 황금 추출물, 구연산나트륨을 혼합하여 제조된 차 음료 및 제조방법이 대한민국 공개특허 제 10-2013-0130588호가 공개되었다. 또, 연잎차 음료 제조방법에 관한 것으로 연잎차 제조공정에 최적화 된 증제, 유념 및 가향 공정을 적용시킴으로써 연잎의 풋내 및 불순물을 제거하고 비타민 C와 생리활성성분을 유지시키면서 기호성 및 생산성을 향상시킨 연잎차 음료 제조방법이 대한민국 등록특허 제 10-0386640호가 공지된 바 있고, 열수 추출한 연근 추출액에 다양한 성분을 적절히 배합하여 항산화 효과와 고지혈증 억제효과를 갖는 연근음료에 관한 제조방법이 대한민국 등록특허 제 10-1142276호가 공지된 바 있다. 그러나 상기문헌 어디에도 연잎, 연자방, 연근, 연줄기 및 연자를 덖음, 당 추출, 효소처리 또는 유산균 발효법 등 다양한 방법을 이용하여 연의 각종 유효성분을 증진시킨 연 음료 및 그 제조방법에 대하여는 개시된 바가 없다. Background Art [0002] Korean Patent Laid-Open No. 10-2010-0008929 discloses a drink and a manufacturing method using a soft drink as a prior art related to a soft drink. The soft drink is extracted with purified water from a yeast, a lotus leaf, Korean Patent Laid-Open No. 10-2013-0130588 has been disclosed as a tea drink prepared by mixing sodium citrate and a preparation method thereof. Also, regarding the method of manufacturing a lotus leaf tea beverage, the lotus leaf which has improved the palatability and productivity while maintaining the vitamin C and physiologically active ingredient by removing the inhabitants and impurities of the lotus leaf by applying the evaporation, A method for manufacturing a tea beverage is disclosed in Korean Patent No. 10-0386640 and a manufacturing method for a root canal beverage having an antioxidative effect and a hyperlipidemia inhibitory effect by appropriately compounding various ingredients in hot water- 1142276 is known. However, none of the aforementioned literatures discloses a soft drink in which various active ingredients of the yeast were promoted by various methods such as a lotus leaf, a soft pot, a lotus root, a soft stem, a soft drink, a sugar extract, an enzyme treatment or lactic acid fermentation .
따라서, 본 발명의 목적은 연잎, 연자방, 연근, 연줄기 및 연자를 덖음, 당 추출, 효소처리 또는 유산균 발효법을 이용한 연 음료를 제공하는데 있다.Accordingly, an object of the present invention is to provide a soft drink using a lotus leaf, a soft pot, a lotus root, a stalks and a soft drink, a sugar extract, an enzyme treatment or a lactic acid fermentation method.
본 발명의 다른 목적은 연잎, 연자방, 연근, 연줄기 및 연자를 덖음, 당 추출, 효소처리 또는 유산균 발효법을 이용한 연 음료의 제조방법을 제공하는데 있다.Another object of the present invention is to provide a method for preparing a soft drink using a lotus leaf, a soft pot, a lotus root, a stalks and a softener, a sugar extract, an enzyme treatment or a lactic acid fermentation method.
본 발명은 상기 목적은 (a) 연잎, 연자방, 연근, 연줄기 및 연자를 수세한 다음 세절하여 원료를 준비하는 단계와; (b) 상기 (a)단계에서 준비한 연잎 또는 연자방을 180 ~ 300℃에서 5 ~ 10분간 덖어내어 덖은 연잎 또는 연자방에 정제수를 첨가하여 연잎 또는 연자방 추출물을 수득하는 단계와; (c) 상기 (a)단계에서 준비한 연근에 황백당을 첨가하여 1, 2차 숙성한 다음 여과하여 연근 당 추출물을 수득하는 단계와; (d) 상기 (a)단계에서 준비한 연근에 엿기름을 첨가하여 효소 처리한 다음 여과하여 효소 처리된 연근 추출물을 수득하는 단계와; (e) 상기 (a)단계에서 준비한 연근, 연줄기 또는 연자에 Lactobacillus brevis 및 Leuconostoc paramesenteroides 중에서 선택되는 어느 하나 아상의 유산균을 접종하여 발효한 다음 여과하여 연근, 연줄기 또는 연자 유산균 발효 추출물을 수득하는 단계와; (f) 상기 (b), (c), (d) 및 (e)단계에서 수득한 추출물 중에서 선택되는 어느 하나에 올리고당, 과당 및 구연산나트륨을 포함하는 첨가물을 배합하여 용기에 충진한 후 밀봉상태에서 살균한 다음 포장하여 연 음료를 제조하는 단계로 이루어지고 상기 제조된 연 음료의 pH, 색도, 플라보노이드 함량 및 생리활성을 측정하고 비교예로 제조한 연잎, 연자방, 연근, 연줄기 및 연자 차 음료와 관능검사를 비교 평가하는 단계를 통하여 달성하였다.The object of the present invention is achieved by a method for preparing a raw material, comprising the steps of: (a) preparing a raw material by washing a lotus leaf, a soft pot, a lotus root, (b) extracting the lotus leaf or the soft palate prepared in the step (a) at 180 to 300 ° C for 5 to 10 minutes and adding purified water to the dried lotus leaf or soft palate; (c) adding yellowish white sugar to the lotus root prepared in the step (a), first and secondly aging and then filtering to obtain a lotus root extract; (d) adding maltose to the rootstock prepared in the step (a), treating with an enzyme, and then filtering to obtain an enzyme-treated lotus root extract; (e) fermenting lactic acid bacteria of any one of A-phase selected from Lactobacillus brevis and Leuconostoc paramesenteroides in the root, stem, or stalk prepared in the step (a) and then filtering to obtain a fermented extract of Lotus root, ; (f) an additive comprising oligosaccharide, fructose and sodium citrate is added to any one selected from the extracts obtained in the above (b), (c), (d) and (e) And then, the pH value, the color value, the flavonoid content, and the physiological activity of the prepared soft drink were measured. The prepared soft drink was measured for its softness, softness, And the comparative evaluation of beverage and sensory test.
본 발명은 연잎, 연자방, 연근, 연줄기 및 연자를 덖음, 당 추출, 효소처리 또는 유산균 발효법을 이용하여 제조한 신규한 연 음료를 제공하여 연의 기능성을 쉽게 섭취하여 국민 건강에 기여할 수 있으며, 풍미가 우수한 연 음료의 제조방법을 제공하여 국내산 농산물을 활용한 다양한 음료 개발을 촉진할 수 있는 우수한 발명이다. The present invention provides a novel soft drink prepared by softening a lotus leaf, a soft lotus root, a rootstock, a stalks and a softener, a sugar extract, an enzyme treatment or a lactic acid fermentation method, It is an excellent invention that can promote the development of various beverages utilizing domestic agricultural products by providing a method of producing a soft drink having excellent flavor.
본 발명은 연 음료 및 그 제조방법을 제공한다.The present invention provides a soft drink and a method of manufacturing the same.
이하, 본 발명을 실시예에 따라 더욱 상세하게 설명한다. 그러나 하기 실시예는 본 발명을 예시하기 위한 것에 불과하므로 본 발명의 권리범위를 한정하는 것으로 의도되지는 않는다.
Hereinafter, the present invention will be described in more detail with reference to examples. However, the following examples are merely illustrative of the present invention and are not intended to limit the scope of the present invention.
[실시예 1] 본 발명 연잎차 음료 제조[Example 1] Production of the present invention lotus leaf tea drink
친환경으로 재배한 연잎(충남 아산시 재배)을 깨끗한 물에 세척한 후 이물질을 제거하고 약 0.5 ~ 2 cm로 얇게 세절한 후 그늘에서 물기를 제거하였다. 세절한 연잎을 기호 및 특성에 맞게 최상의 맛을 내기 위해 180 ~ 300℃ 온도에서 5 ~ 10분 동안 가열하여 3회 이상 덖어(Roasting) 주었다. 덖은 연잎은 추출용 티백(18 x 28cm)에 넣고, 추출탱크에 투입하여 정제수를 넣고 30분 ~ 1시간 추출하였다. 이 때 추출물 제조를 위한 정제수 98.5% ~ 99.5중량%에 연잎 0.5 ~ 1.5중량% 비율로 첨가하는 것이 가장 바람직하였다. 상기 연잎 추출물 99.80 ~ 99.97중량%에 기호를 위한 올리고당, 과당 및 차 음료의 안정제로 구연산나트륨을 포함하여 첨가물 0.03 ~ 0.20중량%를 혼합하여 용기에 충진한 후 밀봉 상태에서 80℃ 이상의 온도에서 20 ~ 25분간 살균한 다음 포장하여 연잎차 음료를 제조하였다.
The environmentally friendly lotus leaf (Asan city cultivation in Chungcheongnam-do) was washed in clean water, and the foreign matter was removed, thinly sliced at about 0.5 to 2 cm, and then drained in the shade. In order to obtain the best taste in accordance with the characteristics and characteristics, the three leaves were heated at 180 ~ 300 ℃ for 5 ~ 10 minutes and roasted more than 3 times. The green leaves were placed in a teabag (18 x 28 cm) for extraction, put into an extraction tank, and added with purified water for 30 minutes to 1 hour. At this time, it was most preferable to add 98.5% ~ 99.5% by weight of purified water for the preparation of the extract at a ratio of 0.5-1.5% by weight of lotus leaf. The composition of the present invention is prepared by mixing 99.80% ~ 99.97% by weight of the extract of the lotus leaf extract with 0.03 ~ 0.20% by weight of additives such as oligosaccharide, fructose and sodium citrate as a stabilizer for tea beverages, filling the container, Sterilized for 25 minutes, and packed to make a lotus leaf tea beverage.
[실시예 2] 본 발명 연자방 차 음료 제조[Example 2] Production of soft drink prepared by the present invention
친환경으로 재배한 연자방(충남 아산시 재배)을 깨끗한 물에 세척한 후 이물질을 제거하고 약 0.5 ~ 2cm로 얇게 세절한 후 그늘에서 물기를 제거하였다. 세절한 연자방을 실시예 1과 동일한 방법으로 연자방 차 음료를 제조하였다.
After cultivated in an environmentally friendly potted plant (cultivated in Asan city, Chungcheongnam-do), it was washed in clean water, and then the foreign matter was removed and thinly sliced at about 0.5 to 2 cm. A soft drink was prepared in the same manner as in Example 1, except that the soft drink was prepared.
[실시예 3] 본 발명 당 추출 연근차 음료 제조[Example 3] Production of extracted root lot tea beverage according to the present invention
친환경으로 재배한 연근(충남 아산시 재배)을 깨끗한 물에 세척한 후 이물질을 제거하고 약 0.5 ~ 2cm로 얇게 세절한 후 그늘에서 물기를 제거하였다. 세절한 연근 300g과 황백당 300g을 계량하고, 저장용기에 연근과 황백당을 50g씩 교대로 층을 이루며 적층하여 투입하되 황백당이 세절된 연근 표면에 묻도록 잘 뿌려주었다. The cultivated lotus root (cultivated in Asan city, Chungnam area) was cleaned with clean water, and then the foreign matter was removed. The mixture was thinly sliced at about 0.5 to 2 cm, and water was removed from the shade. 300 g of the lotus root and 300 g of the white sugar were weighed, and 50 g of lotus root and yellow sugar were alternately layered in the storage container. The yellowish white sugar was sprayed on the surface of the cut lotus root.
(1) 1차 숙성(1) First ripening
저장용기의 윗부분을 잘 밀폐하고, 습기나 직사광선을 피하여 통풍이 잘 되는 곳(그늘 상온; 15 ~ 20℃)에서 약 30일 동안 1차 숙성하였다. 일주일 정도가 지나면 발효가 시작되어 당 추출액이 고이기 시작되면 이때부터 2 ~ 3일에 한 번씩 약 30일간 혼합도구를 이용하여 용기 밑바닥까지 골고루 잘 주어주었다. The upper part of the storage container was sealed well and firstly aged for 30 days in a well-ventilated room (at room temperature; 15 to 20 ° C) avoiding moisture or direct sunlight. After about one week, the fermentation started and the sugar extract started to be high. From this time, it was given to the bottom of the container evenly using the mixing tool for about 30 days once every 2-3 days.
(2) 2차 숙성(2) Second aging
상기 1차 숙성된 연근 당 추출물에서 추출액을 걸러내어 다른 저장용기에 넣어 밀폐한 후 습기나 직사광선이 없고 통풍이 잘되는 곳에서 약 30일 이상 2차 숙성을 하였다.The extracts were filtered out from the first aged root extract of the lotus root extract, sealed in a separate storage container, and then subjected to secondary aging for about 30 days or more in a well-ventilated place without moisture or direct sunlight.
(3) 추출(3) Extraction
상기 2차 숙성된 연근 당 추출액을 거름망을 이용하여 여과시킨 다음 여과된 연근 당 추출액 1.5 ~ 3.5중량%과 정제수 96.5% ~ 98.5중량%를 추출탱크에 넣고 추출하였다.The second aged root extract was filtered using a sieve, and 1.5 to 3.5% by weight of the extract of the extracted lotus root and 96.5 to 98.5% by weight of purified water were added to the extraction tank and extracted.
(4) 음료 배합, 살균 및 포장(4) beverage formulations, sterilization and packaging
상기 연근 추출물 99.80 ~ 99.97중량%에 기호를 위한 올리고당, 과당 및 차 음료의 안정제로서 구연산나트륨을 포함하여 첨가물 0.03 ~ 0.20중량%를 혼합하여 용기에 충진한 다음 밀봉 상태에서 80℃ 이상의 온도에서 20 ~ 25분간 살균한 후 포장하여 당 추출 연근차 음료를 제조하였다.
The mixture of oligosaccharide, fructose, and sodium citrate as a stabilizer for tea beverages is mixed in an amount of 99.80 to 99.97% by weight of the lotus root extract, and the mixture is filled in a container. After sterilization for 25 minutes, it was packaged to prepare sugar extracted root tea beverage.
[[ 실시예Example 4] 본 발명의 효소 처리된 4] The enzyme-treated 연근차Root tea 음료 제조 Beverage Manufacturing
(1) 효소 처리 연근 추출물 제조(1) Production of enzyme-treated lotus root extract
세절된 연근 300g에 정제수 3L를 추출 탱크에 넣고, 85℃에서 30분간 가열한 후 아밀라제 등 전분 분해효소가 많이 포함되어 있는 엿기름을 15중량%로 첨가하여 고루 섞은 후 다시 30분간 반응시킨 다음 15℃ 이하로 냉각 및 여과하여 효소 처리된 연근 추출물을 제조하였다.3 liters of purified water was added to the extracted lotus root at 300 g, and the mixture was heated at 85 ° C for 30 minutes. Then 15% by weight of maltose containing amylase and a large amount of starch decomposing enzyme was added thereto, Or less, to produce an enzyme-treated lotus root extract.
(2) pH 조정 및 여과(2) pH adjustment and filtration
상기 효소 처리된 연근 추출물에 탄산수소나트륨을 가하여 pH 5.5 ~ 7.0으로 조정한 다음 거름망을 이용하여 여과하였다. 상기에서 얻은 여과액은 정제수 84.5% ~ 89.5중량%에 효소 처리된 연근 추출물 10.5 ~ 15.5중량% 비율로 첨가하였다.Sodium hydrogencarbonate was added to the enzyme-treated lotus root extract to adjust the pH to 5.5 to 7.0, followed by filtration using a sieve. The filtrate obtained above was added to the purified water at a ratio of 84.5% to 89.5% by weight in the ratio of enzyme-treated lotus root extract of 10.5 to 15.5% by weight.
(3) 음료 배합, 살균 및 포장(3) beverage formulations, sterilization and packaging
상기 여과된 효소 처리된 연근 추출물 99.80 ~ 99.97중량%에 기호를 위한 올리고당, 과당 및 차 음료의 안정제로서 구연산나트륨을 포함하여 첨가물 0.03~0.20중량%를 혼합하여 용기에 충진한 다음 밀봉 상태에서 80℃이상의 온도에서 20 ~ 25분간 살균한 후 포장하여 효소 처리된 연근차 음료를 제조하였다.
To the filtered enzyme treated lotus root extract, 99.80 to 99.97% by weight of the oligosaccharide, fructose, and sodium citrate as a stabilizer for tea beverages were mixed in an amount of 0.03 to 0.20% by weight and filled in a container. And sterilized for 20 ~ 25 minutes to prepare an enzyme - treated rootstock tea beverage.
[[ 실시예Example 5] 본 발명의 유산균 발효법을 이용한 5] Using the lactic acid fermentation method of the present invention 연근차Root tea 음료 제조 Beverage Manufacturing
(1) 원료 준비 및 유산균 균주 배양(1) Preparation of raw materials and cultivation of lactic acid bacteria strain
친환경으로 재배한 연근(충남 아산시 재배)을 깨끗한 물에 세척하고 물기를 제거한 후 분쇄기를 이용하여 마쇄하였다. The cultivated lotus root (cultivated in Asan city, Chungnam Province) was cleaned with clean water and the water was removed and then ground using a pulverizer.
유산균 균주는 공지된 락토바실러스 브레비스(Lactobacillus brevis) 종균주와 루코노스톡 파라메센테로이즈(Leuconostoc paramesenteroides) 종균주를 사용하고, 배양은 액체배양으로 실시하였다. 즉, 1 L용량의 삼각플라스크에 MRS(Man Rogosa Sharpe) 배양액에 상기 균주 0.1중량%를 접종한 후 진탕 배양기에서 혐기적인 조건 하에서 35 ~ 45℃, 12 ~ 36 시간동안 진탕 배양하였다.The lactic acid bacteria strain was a known strain of Lactobacillus brevis and Leuconostoc paramesenteroides, and the cultivation was carried out by liquid culture. That is, 0.1% by weight of the strain was inoculated in a 1 L Erlenmeyer flask, and cultured under anaerobic conditions at 35 to 45 ° C for 12 to 36 hours with shaking culture.
(2) 유산균 발효 연근 추출액 제조(2) Production of lactic acid bacteria fermented lotus root extract
발효조에 상기 마쇄한 연근 1kg에 정제수 1L를 넣고 균체수가 104 ~107 cfu/g가 되도록 상기 유산균 배양액을 접종한 다음 35 ~ 45℃, 12 ~ 36 시간 동안 배양한 후 거름망으로 여과하여 연근 발효 추출물을 제조하였다.The fermented milk was inoculated with 1 L of the above-mentioned ground lotus root in 1 kg of the ground lotion, and the lactic acid bacteria culture solution was inoculated so that the number of the cells became 10 4 to 10 7 cfu / g, and then cultured at 35 to 45 ° C for 12 to 36 hours. The extract was prepared.
(3) 유산균 발효 연근차 음료 제조(3) Production of lactic acid bacteria fermented root canal tea
상기 유산균 발효 연근 추출물 10.5 ~ 15.5중량%에 정제수 84.30 ~ 89.47중량%와 기호를 위한 올리고당, 과당 및 차 음료의 안정제로서 구연산나트륨을 포함하여 첨가물 0.03 ~ 0.20중량%를 혼합하여 용기에 충진한 다음 밀봉 상태에서 80이상의 온도에서 20 ~ 25분간 살균한 후 포장하여 유산균 발효 연근차 음료를 제조하였다.
The mixture of oligosaccharide, fructose and sodium citrate as a stabilizer for tea beverages and 0.03-0.20% by weight of additives are mixed in 10.5-15.5% by weight of the lactic acid bacteria fermented rootstock extract and filled in a container, , And sterilized for 20 ~ 25 minutes at a temperature of 80 or more, and then packaged to prepare lactobacillus fermented lotus root tea beverage.
[[ 실시예Example 6] 본 발명의 유산균 발효법을 이용한 연줄기 차 음료 제조 6] Manufacture of stover tea beverage using the lactic acid fermentation method of the present invention
연줄기를 사용하여 상기 실시예 5와 동일한 방법으로 본 발명 유산균 발효 연줄기 차 음료를 제조하였다.
The lactic acid fermented starch tea beverage of the present invention was prepared in the same manner as in Example 5 above using a draw line.
[[ 실시예Example 7] 본 발명의 유산균 발효법을 이용한 7] Using the lactic acid fermentation method of the present invention 연자Lecturer 차 음료 제조 Tea beverage manufacturing
연자를 사용하여 상기 실시예 5와 동일한 방법으로 본 발명 유산균 발효 연자 차 음료를 제조하였다.
The inventive lactobacillus fermented tea beverage of the present invention was prepared in the same manner as in Example 5, except that,
[[ 실시예Example 8] 본 발명의 연 혼합 차 음료 제조 8] The production of the soft tea beverage of the present invention
상기 실시예 1의 연잎 추출물 79.60 ~ 81.17중량%, 상기 실시예 3의 연근 당 추출물 9.80 ~ 10.20중량%, 상기 실시예 6의 유산균 발효 연줄기 추출물 4.50 ~ 5.00중량%, 상기 실시예 7의 유산균 발효 연자 추출물 4.50 ~ 5.00중량% 및 기호를 위한 올리고당, 과당류 및 차 음료의 안정제로서 구연산나트륨을 포함하여 첨가물 0.03 ~ 0.20중량%를 혼합하여 용기에 충진한 다음 밀봉 상태에서 80 이상의 온도에서 20 ~ 25분간 살균한 후 포장하여 연 혼합 차 음료를 제조하였다.
The extract of the lotus root extract of Example 1 at 79.60 to 81.17% by weight, the extract of Lotus root of the Example 3 at 9.80 to 10.20% by weight, the lactic acid bacteria fermentation stem extract of Example 6 at 4.50 to 5.00% by weight, Yeast Extracts 4.50 to 5.00% by weight and 0.03 to 0.20% by weight of additives, including oligosaccharides, fructose, and sodium citrate as stabilizers for tea beverages, are filled in a container and then sealed at 20 to 25 After sterilization for a minute, the mixture was packed to prepare a mixed tea beverage.
[[ 비교예Comparative Example 1] One] 연잎차Ocher tea 음료 제조 Beverage Manufacturing
연잎을 깨끗한 물에 세척한 다음 직사광선이 닿지 않는 곳에서 약 0.5 ~ 2cm 정도로 썰어 채반에 엷게 펴고, 바람이 잘 통하는 곳에서 건조시킨 후 잘 건조시킨 연근 10 ~ 15중량%에 정제수 85 ~ 90중량% 첨가하여 80 5분간 가열하여 연잎차 음료를 제조하였다.
The dried lotus leaves were washed with clean water and then thinned to a size of about 0.5 to 2 cm at a place not exposed to direct sunlight and thinned to a wick. The dried lotus root was dried in a well- And heated for 80 minutes to prepare a lotus leaf tea beverage.
[[ 비교예Comparative Example 2] 2] 연자방Lecture room 차 음료 제조 Tea beverage manufacturing
연자방을 사용하여 상기 비교예 1과 동일한 방법으로 연자방 차 음료를 제조하였다.
The soft drink was prepared in the same manner as in Comparative Example 1, except that the soft drink was used.
[[ 비교예Comparative Example 3] 3] 연근차Root tea 음료 제조 Beverage Manufacturing
연근을 사용하여 상기 비교예 1과 동일한 방법으로 연근차 음료를 제조하였다.
A rootstock tea beverage was prepared in the same manner as in Comparative Example 1 using a rootstock root.
[[ 비교예Comparative Example 4] 연줄기차 음료 제조 4] Leading train beverage manufacturing
연줄기를 사용하여 상기 비교예 1과 동일한 방법으로 연줄기 차 음료를 제조하였다.
The pearled tea beverage was prepared in the same manner as in Comparative Example 1, using the draw line.
[[ 비교예Comparative Example 5] 5] 연자Lecturer 차 음료 제조 Tea beverage manufacturing
연자를 사용하여 상기 비교예 1과 동일한 방법으로 연자 차 음료를 제조하였다.
A soft tea beverage was prepared in the same manner as in Comparative Example 1, except that a soft drink was used.
[[ 비교예Comparative Example 6] 연 혼합 차 음료 제조 6] Manufacture of mixed tea beverages
연근, 연줄기, 연잎, 연자 및 연자방을 깨끗한 물에 세척한 다음 직사광선이 닿지 않는 곳에서 약 0.5 ~ 2cm 정도로 썰어 채반에 엷게 펴고 바람이 잘 통하는 곳에서 건조시킨 후 연근, 연줄기, 연잎, 연자 및 연자방을 1:1:1:1:1의 비율로 혼합하여 상기 비교예 1과 동일한 방법으로 연 혼합 차를 제조하였다.
After washing the lotus root, stalks, leaves, stamens, and stamens in clean water, they are sliced at about 0.5 to 2 cm in a place where they are not exposed to direct sunlight, thinned in a wicker, dried in a windy place, A soft mixture was prepared in the same manner as in Comparative Example 1 by mixing the softener and softener in a ratio of 1: 1: 1: 1: 1.
[ 실험예 1] 연 음료 pH 측정 [ Experimental Example 1] Measurement of soft drink pH
상기 실시예 1 ~ 8과 비교예 1 ~ 6의 차 음료의 pH는 pH meter(Metrohm 691, Metrohm UK Ltd., Herisau, Switzerland)를 이용하여 측정하였다. The pH of tea beverages of Examples 1 to 8 and Comparative Examples 1 to 6 was measured using a pH meter (Metrohm 691, Metrohm UK Ltd., Herisau, Switzerland).
pH 측정결과는 표 1에 나타낸 바와 같이 유산균 접종 발효시킨 실시예 5, 실시예 6, 실시예 7 및 실시예 8의 pH는 3.72, 3.49, 3.74 및 4.00을 나타내어 다른 연 음료들에 비해 pH가 낮게 측정된 것을 확인하였고, 나머지 연 음료들의 pH는 6.8 이상으로 측정되었다.
As shown in Table 1, the pH values of the fermented lactic acid bacteria in Example 5, Example 6, Example 7 and Example 8 were 3.72, 3.49, 3.74 and 4.00, respectively, as shown in Table 1, And the pH of the remaining soft drinks was measured to be above 6.8.
[[ 실험예Experimental Example 2] 연 음료 색도 측정 2] Color measurement of soft drinks
상기 실시예 1 ~ 8과 비교예 1 ~ 6의 차 음료의 색도는 UV-visible spectrophotometer(UV-1601, Shimadzu Co., Kyoto, Japan)를 이용하여 L(lightness), a(redness), b(yellowness)를 각각 측정하여 Hunter's color value로 나타내었다.The color of tea beverages of Examples 1 to 8 and Comparative Examples 1 to 6 was measured using a UV-visible spectrophotometer (UV-1601, Shimadzu Co., Kyoto, Japan) yellowness) were measured and expressed as Hunter's color value.
실험결과 표 2에서와 같이 유산균 발효시킨 실시예 6인 연줄기차 음료가 49.82 및 4.60으로 L 및 b값이 가장 높았으며 밝은 황색을 보였고, 실시예 5, 실시예 8의 연음료들을 제외한 나머지 연 음료들은 L값이 7.87 이하로 어두운 황색을 보였다.
The results are shown in Table 2. As shown in Table 2, the train drinks of Example 6, which was fermented with lactic acid bacteria, showed the highest values of L and b of 49.82 and 4.60, and showed a light yellow color. Showed a dark yellow color with an L value of less than 7.87.
[[ 실험예Experimental Example 3] 연 음료의 총 플라보노이드 함량 측정 3] Total flavonoid content of soft drinks
총 플라보노이드는 aluminium nitrate assay 방법에 의거하여 수행하였다. 즉, 시료 100uL, 10% ammonium nitrate용액 100uL, 1 M potassium acetate용액 100uL 및 80% 에탄올 4.7mL를 혼합한 후 25에서 40분간 반응시킨 다음 415 nm에서 흡광도를 측정하였다. 시료 내 총 플라보노이드 함량은 표준 시약인 쿼틴(quercetin)을 사용한 표준곡선을 이용하여 계산하였으며 이때 단위는 quercetin equivalent(QE) ug/g로 하였다.Total flavonoids were assayed by aluminum nitrate assay. That is, 100 μL of sample, 100 μL of 10% ammonium nitrate solution, 100 μL of 1 M potassium acetate solution and 4.7 mL of 80% ethanol were mixed and reacted at 25 ° C. for 40 minutes and then absorbance was measured at 415 nm. The total flavonoid content in the sample was calculated using a standard curve using quercetin as a standard reagent, and the unit was quercetin equivalent (QE) ug / g.
실험결과 표 3에서와 같이 연부위마다 각각 총 플라보노이드 함량이 다르게 측정되었고, 연자로 제조된 차 음료 실시예 7에서 135.62 ug/g로 다른 부위 차 음료보다 높게 측정되었다. 총 플라보노이드 함량이 가장 높게 측정된 음료는 실시예 8로 137.57 ug/g이 측정되어 연 음료 제조시 부위별로 각각 사용하는 것보다 혼합하여 음료를 제조하는 것이 기능성 측면에서 더 효과적인 것으로 판단되었다.
As shown in Table 3, the total flavonoid content of each soft portion was measured differently, and 135.62 ug / g was determined to be higher than that of other portions of tea drink in Example 7 of tea made from soft drink. As a result, it was found that 137.57 ug / g was measured as the highest flavonoid content in Example 8, so that it was more effective in terms of functionality to prepare beverages by mixing them with each other at the time of preparing a soft drink.
(ug quercetin/g of solid)Total flavonoid content
(ug quercetin / g of solid)
[[ 실험예Experimental Example 4] 연 음료의 생리활성 측정 함량 측정 4] Measurement of physiological activity of soft drinks
상기 실시예 1 ~ 8의 차 음료와 비교예 1 ~ 6의 차 음료의 생리활성 측정은 DPPH radical 소거능으로 측정하였다.The measurement of physiological activity of tea beverages of Examples 1 to 8 and tea beverages of Comparative Examples 1 to 6 was measured by DPPH radical scavenging ability.
DPPH(1,1-diphenyl-2-picrylhydrazyl, sigma) 라디칼 소거활성은 Blois방법을 변형하여 측정하였다. DPPH 0.15 mM을 absolute ethanol 100 mL에 용해한 후, 50% ethanol용액을 대조구로 하여 517 nm에서 DPPH용액의 흡광도를 약 1.0이 되도록 희석하여 사용하였다. 시료 0.5 mL에 DPPH용액 5 mL을 혼합하여 정확히 3분 동안 반응시킨 후 UV-visible spectrophotometer(UV-1601)를 이용하여 517 nm에서 흡광도를 측정하여 아래의 식으로부터 DPPH radical 소거활성을 계산하였다.
DPPH (1,1-diphenyl-2-picrylhydrazyl, sigma) radical scavenging activity was measured by modifying the Blois method. 0.15 mM of DPPH was dissolved in 100 mL of absolute ethanol, and the absorbance of DPPH solution was diluted to approximately 1.0 at 517 nm with 50% ethanol solution as a control. The DPPH radical scavenging activity was calculated from the following equation by measuring the absorbance at 517 nm using a UV-visible spectrophotometer (UV-1601) after precisely reacting for 3 minutes with 5 mL of DPPH solution.
여기서 As: Here, As: AbsorbanceAbsorbance of group with sample, Ac : of group with sample, Ac: AbsorbanceAbsorbance of group without sample 이다. of group without sample.
실험결과 표 4와 같이 모든 차 음료에서 DPPH radical 소거능은 60% 이상 나타내어 비교적 높은 항산화능을 보이며, 실시예 8 음료가 93.01%로 가장 높은 항산화능을 나타냈다. 연근차 음료 중에서 유산균 발효법을 이용한 실시예 5 음료에서 82.01%로 높은 항산화능을 나타냈고, 연줄기 및 연자차 음료에서도 유산균 발효법을 이용한 실시예 6과 실시예 7이 비교예 5와 비교예 6보다 더 높은 항산화능을 나타내는 것을 확인 하였다.
As shown in Table 4, DPPH radical scavenging activity of all the tea beverages was 60% or higher, showing a relatively high antioxidative activity, and Example 8 showed the highest antioxidative activity of beverage 93.01%. Example 5 using lactic acid bacteria fermentation method showed high antioxidative activity of 82.01% in roasted tea beverage, and Example 6 and Example 7 using lactic acid bacteria fermentation method also showed higher antioxidative activity than Comparative Example 5 and Comparative Example 6 It was confirmed that it exhibited higher antioxidative ability.
[[ 실험예Experimental Example 5] 연 음료의 관능평가 5] Sensory evaluation of soft drinks
상기 실시예 1 ~ 8의 차 음료와 비교예 1 ~ 6의 차 음료에 대한 관능평가는 패널 30명을 선정하여 실시하였다. 관능 평가항목은 색, 향, 맛, 식감 및 전체적인 기호도에 대해 5점 척도를 기준으로 5점은 매우 좋음, 1점은 매우 좋지 않음으로 평가하였다.
The sensory evaluation of tea beverages of Examples 1 to 8 and tea beverages of Comparative Examples 1 to 6 was conducted by selecting 30 panelists. The sensory evaluation items were evaluated as 5 points for color, aroma, flavor, texture, and overall preference based on 5 point scale, and 1 point for very good.
(1) 연잎차 음료 관능평가 결과(1) Sensory evaluation results of lotus leaf tea
연잎차 음료인 실시예 1과 비교예 1의 관능평가 비교 결과를 표 5에서 나타낸 바와 같이 연잎을 덖어서 차를 제조했을 때에 색, 향, 맛, 식감 및 전체적인 기호도가 더 높게나와 연잎을 덖으면 차의 관능이 좋아지는 것을 확인 할 수 있었다.
The sensory evaluation results of Example 1 and Comparative Example 1, which are lotus leaf tea beverages, are shown in Table 5. As shown in Table 5, when the tea was prepared by cutting the lotus leaf, the color, flavor, taste, texture and overall acceptability were higher, It was confirmed that the sensation of tea was improved.
(2) 연자방차 음료 관능평가 결과(2) Sensory evaluation result
연자방차 음료인 실시예 2와 비교예 2의 관능평가 비교결과 표 6에서 나타낸바와 같이 연자방을 덖어서 차를 제조했을 때에 색, 향, 맛, 식감 및 전체적인 기호도가 더 높게나와 연자방을 덖으면 차의 관능이 좋아지는 것을 확인 할 수 있었다.
As shown in Table 6, when the tea was prepared by mixing the sweet potatoes, the color, flavor, taste, texture, and overall acceptability were higher than those of Example 2 and Comparative Example 2, It is possible to confirm that the sensory characteristics of the tea are improved.
(3) 연근차 음료 관능평가 결과(3) Sensory evaluation result of lotus root tea drink
연근차 음료인 실시예 3, 4, 5 와 비교예 3의 관능평가 비교 결과 표 7에서와 같이 색, 향, 맛, 식감 및 전체기호도가 실시예 3> 실시예 5> 실시예 4> 비교예 1순으로 나타내어 연근을 당 추출하여 차 음료를 제조하였을 때 가장 관능적으로 뛰어난 것으로 확인할 수 있었다.
Flavor, texture, and overall acceptability were evaluated as in Example 7, Example 5, Example 4, Comparative Example 6, and Comparative Example 7, 1, respectively, and it was confirmed that tea extracts were the most sensual when they were extracted from sugar beet.
(4) 연줄기차 음료 관능평가 결과(4) Result of train drink beverage sensory evaluation
연줄기차 음료인 실시예 6과 비교예 4의 관능평가 비교 결과 표 8에서와 같이 연줄기를 유산균으로 발효하여 차를 제조하였을 때 색, 향, 맛, 식감 및 전체기호도가 더 높게나와 연줄기를 유산균발효 하여 차 음료를 제조하면 차 음료의 관능이 좋아지는 것을 확인 할 수 있었다.
As a result of comparing the sensory evaluation results of Example 6 and Comparative Example 4, which are train drinks, the color, flavor, flavor, texture and overall acceptability of the tea were higher when the string was fermented with the lactic acid bacteria as shown in Table 8, Thus, it was confirmed that the tea beverage had better sensory properties when it was prepared.
(5) 연자차 음료 관능평가 결과(5) The result of tea sensory evaluation
연자차 음료인 실시예 7과 비교예 5의 관능평가 비교 결과 표 9에서와 같이 연자를 유산균으로 발효하여 차를 제조하였을 때 색, 향, 맛, 식감 및 전체기호도가 더 높게나와 연자를 유산균 발효하여 차 음료를 제조하면 차 음료의 관능이 좋아지는 것을 확인 할 수 있었다.
As a result of comparing the sensory evaluation results of Example 7 and Comparative Example 5, which are soft tea beverages, the color, flavor, taste, texture and overall acceptability were higher when the tea was fermented with lactic acid bacteria as shown in Table 9, Thus, it was confirmed that the tea beverage had better sensory properties when it was prepared.
(6) 연 혼합차 음료 관능평가 결과(6) Results of sensory evaluation of mixed tea beverages
연 혼합차 음료인 실시예 8과 비교예 6의 관능평가 비교 결과 표 10에서와 같이 색, 향, 맛, 식감 및 전체기호도가 비교예 6보다 실시예 8이 높게 나와 관능적으로 더 우수한 것으로 확인하였다.
As a result of comparing the sensory evaluations of Example 8 and Comparative Example 6 which are annual mixed tea beverages, it was confirmed that the color, aroma, flavor, texture and overall acceptability were higher than that of Comparative Example 6 and higher than that of Comparative Example 6, .
본 발명은 연 음료 제조시 덖음, 당추출, 효소처리 또는 유산균 발효법을 이용하면 기능적, 품질 측면에서 우수한 연 음료를 제공할 수 있는 효과가 있으므로 식품 산업상 매우 유용한 발명인 것이다. The present invention is an extremely useful invention in the food industry because it has an effect of providing a soft drink excellent in terms of function and quality when using soft drink, sugar extraction, enzyme treatment or lactic acid fermentation in the production of soft drinks.
Claims (4)
(a) washing the lotus leaf, the soft pot, the lotus root, the stalks and the softener, and then finely preparing the raw material; (b) extracting a lotus leaf or a sweet potato prepared by the step (a), adding purified water to the dried lotus leaf or soft pot room; (c) adding yellowish white sugar to the lotus root prepared in the step (a), first and secondly aging and then filtering to obtain a lotus root extract; (d) adding 15 wt% of maltose to the root of the root prepared in step (a), treating with enzyme, and then filtering to obtain an enzyme-treated lotus root extract; (e) lactic acid bacteria are inoculated into the rootstocks, stalks or stalks prepared in step (a) and fermented, followed by filtration to obtain a fermented extract of lotus root, stalks, or soft lactic acid bacteria; (f) an additive consisting of oligosaccharide, fructose and sodium citrate is added to at least one selected from the respective extracts obtained in steps (b), (c), (d) and (e) ≪ / RTI > and then packaged.
The method according to claim 1, wherein the step (b) comprises extracting the extract from the extract of the lotus root at a concentration of 79.60 to 81.17% by weight, the extract of the lotus root at 9.80 to 10.20% 4. The method for preparing a soft drink according to claim 1, wherein the sweetener comprises 4.50 to 5.00% by weight of an extract and 0.03 to 0.20% by weight of an additive including oligosaccharide, fructose and sodium citrate.
The method of claim 1, wherein the lactic acid bacteria in step (e) is any one selected from the group consisting of Lactobacillus brevis and Leuconostoc paramesenteroides.
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Cited By (3)
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CN107788518A (en) * | 2017-10-27 | 2018-03-13 | 云南绿界农业科技有限公司 | A kind of formula and processing method of the optimization of lotus leaf ferment |
CN108634055A (en) * | 2018-05-09 | 2018-10-12 | 上海融扬生物技术有限公司 | Functional Hypon teabag drink and preparation method thereof |
KR20230016437A (en) * | 2021-07-26 | 2023-02-02 | 김인자 | Manufacturing method of Wagashi using Blueberry |
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CN107788518A (en) * | 2017-10-27 | 2018-03-13 | 云南绿界农业科技有限公司 | A kind of formula and processing method of the optimization of lotus leaf ferment |
CN108634055A (en) * | 2018-05-09 | 2018-10-12 | 上海融扬生物技术有限公司 | Functional Hypon teabag drink and preparation method thereof |
KR20230016437A (en) * | 2021-07-26 | 2023-02-02 | 김인자 | Manufacturing method of Wagashi using Blueberry |
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