CN111264567A - Sterilization disinfectant, preparation method and application thereof - Google Patents
Sterilization disinfectant, preparation method and application thereof Download PDFInfo
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- CN111264567A CN111264567A CN202010136097.4A CN202010136097A CN111264567A CN 111264567 A CN111264567 A CN 111264567A CN 202010136097 A CN202010136097 A CN 202010136097A CN 111264567 A CN111264567 A CN 111264567A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
- A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
- A01N47/44—Guanidine; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/30—Polygonaceae [Buckwheat family], e.g. red-knees or rhubarb
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Abstract
The invention discloses a sterilization disinfectant, a preparation method and application thereof, wherein the sterilization disinfectant is prepared from the following raw materials in percentage by mass: 2-3% of angelica dahurica, 3.5-5% of fortune eupatorium herb, 1-3% of rhizoma atractylodis, 1.5-3% of menthol, 55-70% of ethanol, 2-6% of chlorhexidine acetate and the balance of deionized water. The ethanol with the functions of disinfection and sterilization in the components destroys the cell wall of the bacteria, acts on the inside of the bacteria to solidify and denature the protein of the bacteria, and hinders the metabolic function of the bacteria. In addition, the angelica dahurica, the eupatorium fortunei and the rhizoma atractylodis are added into the sterilizing disinfectant, umbellized hydrocarbon and neryl acetate contained in volatile oil of the eupatorium fortunei have an inhibiting effect on influenza viruses, the eupatorium fortunei and the rhizoma atractylodis have good inhibiting effects on bacteria and viruses, 1.5-3% of menthol is added to adjust the smell of the sterilizing disinfectant, and the taste is fresh and lasting; the chlorhexidine acetate in the sterilizing disinfectant is added to further enhance the sterilizing effect of the sterilizing disinfectant.
Description
Technical Field
The invention relates to the technical field of disinfection solution, in particular to a sterilization disinfection solution, a preparation method and application thereof.
Background
The existing sterilization and disinfection modes comprise two types: the first type is electronic equipment disinfection method, which directly adopts electronic sterilization equipment with high cost and complex maintenance and ozone sterilization efficiency, but when the electronic equipment is not used, part of users directly contact the electronic equipment to cause anaphylactic reaction, and the electronic equipment disinfection method cannot be popularized and used in household life.
The other one adopts the disinfectant to sterilize, but the current disinfectant in the market has common sterilizing and disinfecting effects, for example, the disinfectant using lactic acid as an effective sterilizing component has no ideal effect on killing viruses and bacteria; the other type of disinfectant has obvious pungent smell in the actual use process, for example, a boiling disinfectant containing formaldehyde or chlorine dioxide has high irritation to human bodies, and volatile components of the disinfectant are even harmful to the health of the human bodies.
Disclosure of Invention
The invention mainly aims to provide a sterilization disinfectant, a preparation method and application thereof, and aims to solve the technical problems of strong irritation and unsatisfactory sterilization effect of the existing disinfectant.
In order to achieve the purpose, the invention provides a sterilization disinfectant, which is prepared from the following raw materials in percentage by mass: 2-3% of angelica dahurica, 3.5-5% of fortune eupatorium herb, 1-3% of rhizoma atractylodis, 1.5-3% of menthol, 55-70% of ethanol, 2-6% of chlorhexidine acetate and the balance of deionized water.
Preferably, the raw materials for preparing the sterilizing and disinfecting liquid also comprise 1-3% of pomegranate bark and 1-3% of giant knotweed.
Preferably, the raw materials for preparing the sterilizing and disinfecting liquid also comprise 3-8% of a pH regulator, and the pH regulator is citric acid or sodium hydroxide.
In addition, the invention also provides a preparation method of the sterilizing and disinfecting liquid, which comprises the following steps:
weighing 2-3% of crushed angelica dahurica, 3.5-5% of eupatorium fortunei, 1-3% of rhizoma atractylodis, 1.5-3% of menthol, 1-3% of pomegranate rind and 1-3% of polygonum cuspidatum according to the mass ratio, uniformly mixing in a container, adding 55-70% of ethanol for wetting, carrying out percolation in the device after sealing and standing for 3-4h, collecting percolate, and carrying out suction filtration to obtain a mixed solution;
and mixing the mixed solution with deionized water and 2-6% of chlorhexidine acetate, and uniformly stirring to obtain the sterilizing disinfectant.
Preferably, the pH value of the sterilizing and disinfecting liquid is 6-6.8.
Preferably, the percolation speed in the percolation step is 4.2-5 ml/min.
In addition, the invention also provides application of the sterilizing and disinfecting liquid, wherein the using temperature of the sterilizing and disinfecting liquid is 20-50 ℃.
The sterilizing disinfectant disclosed by the invention has the following beneficial effects: the ethanol and the deionized water with the disinfection and sterilization effects in the components can destroy cell walls of bacteria to act inside the bacteria to solidify and denature proteins of the bacteria and hinder the metabolic function of the bacteria, and meanwhile, the ethanol has a good extraction effect on angelica dahurica, eupatorium fortunei, rhizoma atractylodis and menthol, can meet the quick drying effect and has low irritation. In addition, the angelica dahurica, the eupatorium fortunei and the rhizoma atractylodis are added into the sterilizing disinfectant, umbellized hydrocarbon and neryl acetate contained in volatile oil of the eupatorium fortunei have an inhibiting effect on influenza viruses, the eupatorium fortunei and the rhizoma atractylodis have good inhibiting effects on bacteria and viruses, 1.5-3% of menthol is added to adjust the smell of the sterilizing disinfectant, and the taste is fresh and lasting. The chlorhexidine acetate in the sterilizing disinfectant is added to further enhance the sterilizing effect of the sterilizing disinfectant.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In addition, technical solutions between various embodiments may be combined with each other, but must be realized by a person skilled in the art, and when the technical solutions are contradictory or cannot be realized, such a combination should not be considered to exist, and is not within the protection scope of the present invention.
The invention provides a sterilization disinfectant. The sterilization disinfectant in the embodiment can be used for sterilization and disinfection in places such as hospitals and markets in daily life and crowded places, prevents personnel from virus infection, has excellent sterilization effect when being sprayed in air or smeared on the ground, has mild components, and is free of corrosivity and irritation when being used by users.
In one embodiment of the invention, the sterilizing disinfectant is prepared from the following raw materials in percentage by mass: 2-3% of angelica dahurica, 3.5-5% of fortune eupatorium herb, 1-3% of rhizoma atractylodis, 1.5-3% of menthol, 55-70% of ethanol, 2-6% of chlorhexidine acetate and the balance of deionized water.
The main components of the sterilizing disinfectant are ethanol and deionized water with sterilizing effect, high-concentration ethanol can destroy cell walls of bacteria to act inside the bacteria to solidify and denature proteins of the bacteria and hinder metabolic function of the bacteria, and meanwhile, the ethanol has a good extraction effect on angelica dahurica, eupatorium fortunei, rhizoma atractylodis and menthol, can meet a quick drying effect, and is low in irritation. In addition, the angelica dahurica, the eupatorium fortunei and the rhizoma atractylodis are added into the sterilizing disinfectant, umbellized hydrocarbon and neryl acetate contained in volatile oil of the eupatorium fortunei have an inhibiting effect on influenza viruses, the eupatorium fortunei and the rhizoma atractylodis have good inhibiting effects on bacteria and viruses, 1.5-3% of menthol is added to adjust the smell of the sterilizing disinfectant, and the taste is fresh and lasting. The chlorhexidine acetate in the sterilizing disinfectant is added to further enhance the sterilizing effect of the sterilizing disinfectant. Chlorhexidine acetate is used as an excellent bactericide, has a sterilization and destruction effect on candida albicans, escherichia coli and part of staphylococcus, can effectively kill bacterial propagules, can change the permeability of a bacterial cell serous membrane to cause rupture and damage of a bacterial cell membrane, and enables cytoplasm components inside the cell membrane to flow out, so that the chlorhexidine acetate has a sterilization effect, can inhibit synthesis of deoxygenase and oxidase inside bacteria to enable the deoxygenase and oxidase to generate metabolic disturbance, so that bacteria die, and has a good sterilization effect.
Furthermore, the raw materials for preparing the sterilizing and disinfecting liquid also comprise 1-3% of pomegranate bark and 1-3% of giant knotweed. Therefore, the pomegranate bark and the giant knotweed are used as natural bactericidal substances, the sources are wide, the adverse reaction is small, and a good antibacterial effect is achieved, wherein the pomegranate bark contains a large amount of tannin which can solidify protoplasm in a microorganism body, meanwhile, a plurality of enzymes are added into the bactericidal disinfectant to inhibit bacteria and fungi, and the giant knotweed can inhibit the synthesis of intracellular substances of bacteria such as protein and glucose, so that the metabolic speed of bacteria is slowed down, the bacteria are inhibited from growing to die, the skin is not stimulated by adopting natural components, and the damage and the allergic phenomenon of the skin caused by the common disinfectant can be reduced.
Further, the raw materials for preparing the sterilizing and disinfecting liquid also comprise 3-8% of a pH regulator, and the pH regulator is citric acid or sodium hydroxide. Because the service environment of the sterilizing disinfectant needs to be kept in a proper acid-base range to prevent the pH value from being too high or too low to influence the sterilizing effective components in the components, and the too high and too low pH values can cause stimulation effect on the skin, the pH regulator is added into the sterilizing disinfectant to regulate the pH value, the specific sterilizing disinfectant is citric acid and sodium hydroxide, the raw materials are easy to obtain, the production cost is low, and the pH value of the sterilizing disinfectant can be effectively neutralized and regulated.
The invention also provides a preparation method of the sterilizing and disinfecting liquid, which comprises the following steps: weighing 2-3% of crushed angelica dahurica, 3.5-5% of eupatorium fortunei, 1-3% of rhizoma atractylodis, 1.5-3% of menthol, 1-3% of pomegranate rind and 1-3% of polygonum cuspidatum according to the mass ratio, uniformly mixing in a container, adding 55-70% of ethanol for wetting, carrying out percolation in the device after sealing and standing for 3-4h, collecting percolate, and carrying out suction filtration to obtain a mixed solution; and mixing the mixed solution with deionized water and 2-6% of chlorhexidine acetate, and uniformly stirring to obtain the sterilizing disinfectant.
Specifically, all the traditional Chinese medicinal materials are firstly treated, the steps of crushing, wetting and percolating are mainly carried out, the percolation method is adopted to ensure that volatile oil components in the components such as angelica dahurica, eupatorium fortunei, rhizoma atractylodis and the like in the components are not lost, the traditional Chinese medicinal materials are required to be completely immersed by standing before percolation, the components are completely dissolved in an ethanol solution, diffusion is more sufficient, the concentration of the percolation liquid can reach the maximum value, waste is avoided, the percolation liquid containing effective sterilization components is subjected to suction filtration to obtain mixed liquid without solid impurities, in the subsequent steps, the mixed liquid and 2-6% of chlorhexidine acetate are mixed, and deionized water is added to obtain the final sterilization disinfectant.
Further, the pH value of the sterilizing and disinfecting liquid is 6-6.8. It can be understood that the sterilizing effect of the sterilizing and disinfecting liquid prepared by the invention is increased in a weakly alkaline environment, but the stability of other components of the sterilizing and disinfecting liquid is affected by the excessively high alkalinity, the effective sterilizing components are reduced, the decomposition of the components is accelerated, turbidity and even precipitation appear in the sterilizing and disinfecting liquid, the product quality is affected, and the irritation to the skin is increased when the sterilizing and disinfecting liquid is used by a user, so that the pH value of the sterilizing and disinfecting liquid is 6-6.8, the sterilizing and disinfecting liquid in the pH value range is stable and good under the storage condition, the transparency is good, and the content change of the components is small.
Further, the percolation speed in the percolation step is 4.2-5 ml/min. Therefore, as various traditional Chinese medicinal materials for preparing the sterilizing disinfectant have different medicinal properties, the traditional Chinese medicinal materials need to be percolated at a proper speed during percolation so as to ensure that the medicinal properties of the traditional Chinese medicinal materials are optimal, when the percolation speed exceeds 5ml/min, effective components in the components of the traditional Chinese medicinal materials cannot seep out or diffuse in time, the content of the effective components of the finally worthy sterilizing disinfectant is low, the sterilizing effect of a product is poor, when the percolation speed is too slow, namely less than 4.2ml/min, the utilization rate of a percolation device is low, the preparation period of the sterilizing disinfectant is obviously prolonged, and the industrial production is not facilitated.
In addition, the invention also provides application of the sterilizing and disinfecting liquid, wherein the using temperature of the sterilizing and disinfecting liquid is 20-50 ℃. The sterilizing disinfectant has a wide application range, can be used in different fields such as medical treatment and the like, but the effective components can be reduced under the conditions of excessively high temperature and excessively low temperature, so that the application temperature of the sterilizing disinfectant is 20-50 ℃, the content of the effective components in the temperature range is stable, and when the temperature is higher than 50 ℃ or lower than 25 ℃, the effective components in the sterilizing disinfectant can be reduced by about 6 percent, thereby affecting the continuous use of products.
The technical solutions of the present invention are further described in detail with reference to the following specific examples, which should be understood as merely illustrative and not limitative.
Example 1
The preparation of the sterilizing disinfectant comprises the following steps:
weighing 3% of crushed angelica dahurica, 5% of eupatorium, 1% of rhizoma atractylodis, 1.5% of menthol, 1.5% of pomegranate rind and 2% of polygonum cuspidatum according to the mass ratio, uniformly mixing in a container, adding 65% of ethanol for wetting, carrying out percolation in a device after sealing and standing for 3 hours, collecting percolate, and carrying out suction filtration to obtain a mixed solution;
and mixing the mixed solution with 18% of deionized water and 3% of chlorhexidine acetate, and uniformly stirring to obtain the sterilizing disinfectant.
Example 2
The preparation of the sterilizing disinfectant comprises the following steps:
weighing 2% of crushed angelica dahurica, 4% of eupatorium fortunei, 2% of rhizoma atractylodis, 2% of menthol, 2% of pomegranate rind and 3% of polygonum cuspidatum according to the mass ratio, uniformly mixing in a container, adding 55% of ethanol for wetting, sealing and standing for 4 hours, then percolating in a device, collecting percolate, and performing suction filtration to obtain a mixed solution;
and mixing the mixed solution with 24% of deionized water and 6% of chlorhexidine acetate, and uniformly stirring to obtain the sterilizing disinfectant.
Example 3
The preparation of the sterilizing disinfectant comprises the following steps:
weighing 2.5% of crushed angelica dahurica, 3.5% of eupatorium fortunei, 3% of rhizoma atractylodis, 3% of menthol, 3% of pomegranate rind and 1% of polygonum cuspidatum according to the mass ratio, uniformly mixing in a container, adding 70% of ethanol for wetting, carrying out percolation in a device after sealing and standing for 3 hours, collecting percolate, and carrying out suction filtration to obtain a mixed solution;
and mixing the mixed solution with 12% of deionized water and 2% of chlorhexidine acetate, and uniformly stirring to obtain the sterilizing disinfectant.
In order to better illustrate the beneficial effects of the embodiments of the present invention, the present invention purchases a common disinfectant solution with ethanol as a bactericidal component on the market as a comparative example, and the bacteriostatic performance testing parameters of the embodiments 1 to 3 and the comparative example are shown in the following table 1:
| example 1 | Example 2 | Example 3 | Comparative example | |
| Staphylococcus aureus/mm | 23.4 | 22.9 | 23.8 | 18.9 |
| Escherichia coli/mm | 19.5 | 20.2 | 19.8 | 12.3 |
| Candida albicans/mm | 14.5 | 14.9 | 14.0 | 11.5 |
The detection mode is as follows: the sterilization filter paper with the diameter of 6mm is placed into the sterilization disinfectant and the common disinfectant of the embodiment 1-3 to be soaked for 2h, the soaked filter paper is dried and then respectively attached to bacterium-containing plates of staphylococcus aureus, escherichia coli and candida albicans, the plates are cultured in a constant temperature incubator at 36 ℃ for 12h at constant temperature, and the diameter of a bacteriostatic circle is measured by a caliper to detect the bacteriostatic effect.
The sterilization rate test parameters of examples 1 to 3 and comparative example are shown in the following table 2:
the detection method comprises the following steps: under the condition of normal temperature, the sterilization disinfectant and the common disinfectant are used for spraying 40ml of the sterilization disinfectant on an experimental point, the sterilization time is 12 hours, 1 point is taken every 2m on the diagonal line of the experimental point for sampling before and after sterilization, each sampling point is repeatedly sampled for 3 times, a 9cm nutrient agar plate is used for sedimentation for 5min for sampling, the nutrient agar plate is covered after sampling and is placed into a bacteria culture chamber, the culture is carried out for 48 hours under the condition of 37 ℃, the number of bacteria is calculated, and the bacteria reduction rate is calculated.
The above description is only a preferred embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications and equivalents of the present invention, which are made by the present specification and directly/indirectly applied to other related technical fields within the spirit of the present invention are included in the scope of the present invention.
Claims (7)
1. The sterilizing disinfectant is characterized by being prepared from the following raw materials in percentage by mass: 2-3% of angelica dahurica, 3.5-5% of fortune eupatorium herb, 1-3% of rhizoma atractylodis, 1.5-3% of menthol, 55-70% of ethanol, 2-6% of chlorhexidine acetate and the balance of deionized water.
2. The sterilizing and disinfecting solution of claim 1, wherein the raw materials for preparing the sterilizing and disinfecting solution further comprise 1-3% of pomegranate rind and 1-3% of giant knotweed.
3. The sterilizing and disinfecting solution of claim 1, wherein the raw materials for preparing the sterilizing and disinfecting solution further comprise 3-8% of a pH regulator, and the pH regulator is citric acid or sodium hydroxide.
4. A method for preparing the sterilizing and disinfecting liquid as claimed in any one of claims 1 to 3, which comprises the steps of:
weighing 2-3% of crushed angelica dahurica, 3.5-5% of eupatorium fortunei, 1-3% of rhizoma atractylodis, 1.5-3% of menthol, 1-3% of pomegranate rind and 1-3% of polygonum cuspidatum according to the mass ratio, uniformly mixing in a container, adding 55-70% of ethanol for wetting, carrying out percolation in the device after sealing and standing for 3-4h, collecting percolate, and carrying out suction filtration to obtain a mixed solution;
and mixing the mixed solution with deionized water and 2-6% of chlorhexidine acetate, and uniformly stirring to obtain the sterilizing disinfectant.
5. The method of claim 4, wherein the pH of the disinfecting solution is 6-6.8.
6. The method for preparing sterilizing and disinfecting liquid as claimed in claim 4, wherein the speed of percolation in the percolation step is 4.2-5 ml/min.
7. The use of the disinfecting and sterilizing solution of any one of claims 1 to 3, wherein the disinfecting and sterilizing solution is used at a temperature of 20 to 50 ℃.
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| CN112471178A (en) * | 2020-11-03 | 2021-03-12 | 雁知堂中医药科技(山西)有限责任公司 | Traditional Chinese medicine incense formula for avoiding plague and preventing epidemic |
| CN113273582A (en) * | 2021-02-09 | 2021-08-20 | 安徽省荣昌新材料科技有限公司 | Novel antibacterial and antiviral preparation, resin, decorative paper and plate |
| CN115152806A (en) * | 2022-08-26 | 2022-10-11 | 上海瑞帝安生物科技有限公司 | Chinese herbal medicine air disinfectant and preparation method thereof |
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