CN101461558B - Aspergillus bacteriostatic agent and use thereof - Google Patents
Aspergillus bacteriostatic agent and use thereof Download PDFInfo
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- CN101461558B CN101461558B CN200710151182.2A CN200710151182A CN101461558B CN 101461558 B CN101461558 B CN 101461558B CN 200710151182 A CN200710151182 A CN 200710151182A CN 101461558 B CN101461558 B CN 101461558B
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Abstract
The invention relates to a bacteriostatic agent for aspergillus, whose active ingredient is rose water extract. The rose flower water is essence of rose prepared by whole rose extracting, distilling, rose oil preserving, which has inhibition function to aspergillus. The water can be used in beverage, food additive, health-care product and medicament of homo-source, or natural clean fresh-keeping agent. The invention has low preparation cost, strong adaptation, easy operation, wide application prospect.
Description
Technical field
The present invention relates to Aspergillus bacteriostatic agent, specifically rose is exposed at the application suppressing in Aspergillus, can be used in health care, preserving fruit and vegetable utilizing or integration of drinking and medicinal herbs natural health-care products.
Background technology
Rose (Rosa rugosa Thumb.) is the rose family, the machaka of Rosa.Originate in China, plant with a long historyly, as far back as Han dynasty, just have the record about rose.Rosa Rugosa Resources distributes wide, and south gets Guangdong, north is to Liaoning to Heilungkiang, and from the east of coastal area, there is part cultivation in west to inland.Wherein take Gansu, the ground such as Shandong, Beijing, Liaoning, Heilungkiang, Taiwan and Xinjiang is as main.
The rose of areas of Shenyang plantation has: cold-resistant, impoverishment tolerant, disease-resistant, drought resisting, strong adaptability, and manageability, growth is fast, the feature at florescence long (May-October), full-bloom stage can maintain more than 15 years.
Rose can not only be beautified, afforest city and also be integrated medicinal, edible, is widely used in the fields such as chemical industry, food, wine brewing, medicine, nutrient and healthcare products.The Chinese Ancient Books pharmacopeia < < detailed outline > > that picks up any lost article from the road carries: the effect of " rose and blood, promoting circulation of blood regulate the flow of vital energy menstruation regulating, looks beauty treatment ".
Rose contains abundant nutriment, and as vitamin A, B, C, wherein ascorbic content is the abundantest, every 100g contains 2000mg, its content is apple more than 700 times, more than 20 times of sea-buckthorn, than Chinese mud monkey peach, also exceed more than 8 times, can claim the king of vitamin C.The constant that contains protein, fat, carbohydrate, soluble sugar, alkaloid and needed by human, micro-sulphur, phosphorus, potassium, calcium, magnesium, iron, zinc, copper, manganese etc.It also contains ten several amino acids in addition, wherein 5 kinds of essential amino acids, lysine, threonine, a word used in person's names propylhomoserin, isoleucine and phenylalanine.Produce " rosolio " 45 countries that distribute all over the world with fresh rose flower, and obtain repeatly special honours, enjoy the good reputation of " Chinese brandy ".
The rose of this tool natural green, has broad application prospects, and more and more enjoys domestic and international people's favor.
Now, the leading products of rose are to extract Rosa Damascana, are a kind of natural essences of preciousness.Once there were two attars of rose of report just can make 1 kilogram of first-class perfume.Attar of rose is one of worldwide famous and precious essential oil, price up to 7000-8000 dollar/kilogram, have the title of " liquid golden ".
About the chemical composition of rose flavored juice, Rosa Damascana, existing people has done comprehensive research to states such as Bulgaria, Turkey, the Soviet Union.Yu Zhen etc. have analyzed the chemical composition of Rosa gallica oil, Morocco's attar of rose, Gansu bitter water rose oil and Pingyin, Shandong attar of rose, respectively identified 79,74,45 and 54 components, and compare with document, its main chemical compositions is citronellol, geraniol, benzyl carbinol, fanesol and ester class thereof, linalool, rose oxide, eugenol, methyl eugenol, but due to the number on content.And the difference in chemical composition, make the fragrance of these several attars of rose produce delicate discrepancy.And Wu Qiuxias etc. are studied the composition of Pingyin Rosa Damascana, and the lightweight essential oil reclaiming in uncondensable tail gas from still-process is analyzed, find that lightweight derived essential oil is generally partially light, heavy constituent is little, especially suitable, an anti-rose oxide is up to 8.8%, this is extremely valuable light composition, makes the fragrance of lightweight essential oil more strong.Liu Ji just waits Pingyin Rosa Damascana has been carried out to pre-separation, identifies 393 kinds of compounds, and 148 kinds of compounds of quantitative analysis, have done contrast with other regional Rosa Damascanas, and have tested physical and chemical index [Xibei Forest College's journal 2004,19 (4); 138-141].
Summary of the invention
The object of the present invention is to provide a kind of Aspergillus bacteriostatic agent and application thereof, rose liquid distilled from honeysuckle flowers or lotus leaves is inhibited to Aspergillus, its can be made into there is drink, the natural additive for foodstuff of bacteriostasis, health products or medicine, the natural clean antistaling agent etc. of integration of drinking and medicinal herbs.
For achieving the above object, the technical solution used in the present invention is:
A kind of Aspergillus bacteriostatic agent, its active ingredient is rose wate extract, is entirely to be spent through the rose elite rose liquid distilled from honeysuckle flowers or lotus leaves extracting, all active ingredients of attar of rose are produced and retained in distillation by rose, inhibited to Aspergillus.
Described rose liquid distilled from honeysuckle flowers or lotus leaves, be by petal, pistil and/or the holder of rose through extracting, distillation produces, it has retained all active ingredient of rose, what obtain is the elite of rose, therefore be called rose liquid distilled from honeysuckle flowers or lotus leaves; Its sense organ sense of smell has the fragrance similar to fresh-rose, taste is dense, turbid clearly and not, and the another people of news is relaxed and joyful.
Described Aspergillus is other bacterium of these floras such as aspergillus flavus, aspergillus oryzae, Aspergillus terreus, aspergillus nidulans, aspergillus fumigatus, aspergillus niger or yellow bright aspergillus.
The extracting method of described rose liquid distilled from honeysuckle flowers or lotus leaves is steam distillation, ultrasonic extraction or Microwave Extraction method; The extraction raw material rose adopting is petal, pistil and/or holder.The bacteriostasis rate of the rose liquid distilled from honeysuckle flowers or lotus leaves extracting is 50-90%.
1) described steam distillation is, get fresh-rose, with feed liquid weight ratio 1: 20～40 extraordinarily water pack in the distiller of being furnished with condensing unit, add thermal distillation 2～5 hours, by utilizing high-temperature vapor, after making oily essence, spices in rose distill, gasify to make it to liquefy completely by condensation with steam, obtain rose liquid distilled from honeysuckle flowers or lotus leaves in totally-enclosed situation, can collect storage by each generic request.
2) described ultrasonic extraction is, get rose with solid-liquid ratio 1: 15～30 extraordinarily water carry out ultrasonic extraction, operating frequency 20～40khz, electric power 100～400w, temperature 30～80 degree, 10～45 minutes ul-trasonic irradiation time (rose at this moment by ultrasound assisted extraction technique make its cell rupture inclusion be released in feed liquid), and then by steam distillation 2～3 hours, obtain rose liquid distilled from honeysuckle flowers or lotus leaves; The rose flavored juice amount obtaining is suitable with the amount that distillation is produced, but the content of its most of nutritional labeling has all increased several percentage points.
3) described Microwave Extraction method is, get rose with extraordinarily water of solid-liquid ratio 1: 15～20, microwave power 100～400w, temperature 30～80 degree, 20～50 minutes time can make rapidly the cell membrane of rose penetrate in the scope of this action function, and intracellular all kinds of chemical composition is released among this feed liquid thereupon, and then by steam distillation 2～3 hours, obtain rose liquid distilled from honeysuckle flowers or lotus leaves.
Rose liquid distilled from honeysuckle flowers or lotus leaves is inhibited to Aspergillus, its can be made into there is drink, natural additive for foodstuff and the antioxidant of bacteriostasis, health products, medicine or the natural clean antistaling agent etc. of integration of drinking and medicinal herbs.
As natural fruit, dredge antistaling agent, the fruits and vegetables that may be used on plucking are if the sprinkling of apple, pears, peach, Lemon fruit, cucumber, capsicum etc. is to extend their freshness date; Meanwhile, the fragrant breath itself having, after fruits and vegetables are edible, makes it pleasant impression more;
As natural detergent. can be used for being prepared into the clean toothpaste of health care and health care cleaning agent, directly take and can remove halitosis peculiar smell, allow people left a lingering fragrance in one's mouth.
As natural detergent, it is capable of purifying air, and is widely used in household, office building hotel, public place, office, computer desk, the peculiar smell of the interior removing of the logical instrument such as desk, carplane on hand, fresh air, sterilizing etc.;
As natural additive for foodstuff and antioxidant, may be used in bakery product, cake, custard tart, moon cake or rice cake that preparation has rose fragrance, play antioxygen, anti-corrosive fresh-keeping effect.
As drink, natural additive for foodstuff, rose liquid distilled from honeysuckle flowers or lotus leaves can be prepared into various food, the nutrient and healthcare products such as cake, candy, nutrition soup, drink (being that nutrition and health care is revealed), tea, rosolio;
As medicine, can prepare and there is the heart of protecting profit spleen, activate, stimulate circulation, strengthening vessel wall elasticity, reduces the medicine of the congested phenomenon of heart, lowers cardiopathic incidence; Improve spleen function, the sharp spleen benefit heart.
As the first-class oral rose flavored juice of integration of drinking and medicinal herbs health products, except having the most pleasant fragrant fragrance, also be rich in hundreds of nutritional labelings that are of value to health, as multivitamin, glucose, fructose, citric acid, malic acid ..., be conducive in a large number antidotal terpene substances (being rich in citronellol etc. and part vinyl compound), multiple NMF (the tartaric acid class containing and the vitamin substances being rich in) is human body the most intimate " Mininurse next to the skin ".Can be prepared into and there is very strong nourishing body and mind, can improve body immunity, regulate hormone level to promote the integration of drinking and medicinal herbs health products of cyclic metabolism, for improving and strengthen urinary system flesh energy, diuresis, strong kidney, promoting that toxin is mediated, metabolism, nourishing YIN for benefiting the kidney.
Rose liquid distilled from honeysuckle flowers or lotus leaves can be prepared the product with integration of drinking and medicinal herbs and pharmacological action: the chemical composition in rose liquid distilled from honeysuckle flowers or lotus leaves: benzyl carbinol, nerol, nerolidol, eugenol etc. have removing toxic substances, the effect such as hypotensive, calm; Citronellol, geraniol and geranial have sterilization, anti-inflammatory, antidepression, short absorption, invigorate blood circulation and sedation; Benzyl carbinol, geraniol, citronellol, nerol have stimulating the menstrual flow, the effects such as calmness of sleeping peacefully.Eugenol not only has blood circulation-promoting functions but also has adjusting action of enterogastric function simultaneously.
In addition, in rose liquid distilled from honeysuckle flowers or lotus leaves, contained Flavonoid substances is: ten triketones, aristolone, 2-undecyl ketone etc., and by hydroxylating, make cell hydrolysis lose carcinogenic activity, play anticancer effect.Therefore it has antitumor action, and resisting cardiovascular disease increases CF and cerebral blood flow (CBF), reduces blood pressure, and can effectively prevent and treat the cardiovascular and cerebrovascular disease of hypertension and coronary heart disease etc.
Advantage of the present invention:
1. product cost of the present invention is low, practical, and easy operating is produced, and properties of product are full and accurate reliable.
2. as good merchantable brand, the natural nutrition food supplement of first-class beautifying face and moistering lotion, enjoy people to praise highly, particularly the first-class beautifying tonic product of women.Especially find that she has after the inhibitory action of Aspergillus, its rose liquid distilled from honeysuckle flowers or lotus leaves range of application is increased, field is widened, value is strengthened.
1) be applied to bake etc. in food and there is anti-oxidant, anti-corrosive fresh-keeping effect.
2) as the natural clean antistaling agent of plucking vegetables, fruit.
3) as natural detergent. can be used for being prepared into the clean toothpaste of health care and health care cleaning agent, directly take and can remove halitosis peculiar smell, allow people left a lingering fragrance in one's mouth.
4) as medicine, can be prepared into and there is menstruction regulating and pain relieving effect, can also comprehensive adjusting female reproductive system function, strengthen Monitoring Ovarian Function, regulate estrogen and lutern secretion, adjust the menstrual cycle, and reduce too much menses, calm premenstrual tension; Can activate blood circulation and disperse blood clots, restore menstrual flow and invigorate blood circulation, effectively improve dysmenorrhoea, irregular menses etc.
3. the bacteriostasis having, at room temperature preservation of the rose flavored juice of preparing for up to 2 years should be powerful, allow people apply relieved.
4. people, day by day pursue environmental protection, uphold nature strengthens today of nutrition, can be used as the product rose liquid distilled from honeysuckle flowers or lotus leaves of the pharmacological action of natural clean antistaling agent, natural food additive, integration of drinking and medicinal herbs, has boundless market application foreground.
Accompanying drawing explanation
Fig. 1 is the Part of photos taken of bacteriostatic test of the present invention in embodiment 1;
Fig. 2 is the Part of photos taken of bacteriostatic test of the present invention in embodiment 4.
The specific embodiment
Embodiment 1
Rose flavored juice produce as follows:
Get new fresh-rose 1000g, amount of water is 25 times of flower weight, and the rose liquid distilled from honeysuckle flowers or lotus leaves of producing after steam distillation 4h is carried out in heating, and its main component is as shown in table 1.
After all fractions are mixed into one, place the rose liquid distilled from honeysuckle flowers or lotus leaves 6# of month, place rose liquid distilled from honeysuckle flowers or lotus leaves 12#, the storage rose liquid distilled from honeysuckle flowers or lotus leaves 11# of 2 years of 1 year.
2. bacteriostatic test
The indicator bacteria Aspergillus of selecting (yellow bright aspergillus and aspergillus niger) is carried out to rejuvenation cultivation, and its incubation step is:
1) preparation is applicable to the culture medium of indicator bacteria growth, is applicable to the culture medium of Aspergillus growth, and the culture medium that is applicable to growth according to routine is Cha Shi culture medium, matrix weight proportion: NaNO.3%, KHPO
40.1%, Mgso
4.7H
2o0.05%, Feso
4.7H
2o0.001%, KCl0.05% sucrose 3.0%, agar 1.8-2.0%, distilled water 100ml, PH 6.7-6.8; 1 atmospheric pressure sterilizing of the culture medium preparing, after 30 minutes, is prepared to inclined-plane standby;
2) bacterial classification is seeded in the oblique culture medium preparing again, is placed in the 28 ℃ of constant temperature culture of temperature 5 days that are applicable to its growth;
3) it is stand-by that bacterial classification is diluted to the bacteria suspension of 100 times after cultivating;
4) adopt dull and stereotyped scraps of paper determination method, detect the rose liquid distilled from honeysuckle flowers or lotus leaves of the difference storage mode of producing by distillation;
The dull and stereotyped scraps of paper determination method of described employing, first preparation is mixed with the nutrient matrix flat board of each indication bacterial classification, again the 1em scraps of paper of the bacterium of having gone out in advance are immersed respectively to above-mentioned all kinds of rose dew retting to be measured after 10 minutes, take out respectively and put into the 2-6 piece of scraps of paper matrix flat board that scribbles indication mushroom, insert respectively constant temperature culture, 28 ℃ of cultivation temperature, cultivate 5 days, survey the bacteriostatic diameter D that it suppresses indicator bacteria;
The bacteriostatic diameter of 6# rose liquid distilled from honeysuckle flowers or lotus leaves: yellow bright Aspergillus D=32mm; Aspergillus niger D=18.5mm
The bacteriostatic diameter of 11# rose liquid distilled from honeysuckle flowers or lotus leaves: yellow bright Aspergillus D=12.5mm aspergillus niger D=10mm
The bacteriostatic diameter of 12# rose liquid distilled from honeysuckle flowers or lotus leaves: yellow bright Aspergillus D=28mm aspergillus niger D=10mm
The rose liquid distilled from honeysuckle flowers or lotus leaves making can be used as for natural detergent, capable of purifying air, for bedroom and the concentrated place of crowd; Can prepare again anti-inflammatory emollient, for antipruritic etc. the anti-allergic effects of bite by mosquitos and allergic skin.
Embodiment 2
The rose liquid distilled from honeysuckle flowers or lotus leaves that adopts ultrasonic wave extractive distillation method to produce, can be prepared into the oral rose flavored juice of function sex-health products.
Rose flavored juice produce as follows:
Take fresh-rose 1000g, amount of water is 30 times of flower, and with ultrasonic wave extraction, selection work frequency is 28.5KHZ, and electric power is 130W, and the ul-trasonic irradiation time is 30min, and temperature is 70 ℃.Rose, under ultrasound assisted extraction technique, breaks rapidly its cell, and inclusion discharges thereupon, and then after way of distillation distillation 2h, the rose liquid distilled from honeysuckle flowers or lotus leaves making, its main component is as shown in table 1;
After all fractions are mixed into one, place the rose liquid distilled from honeysuckle flowers or lotus leaves 6# of month, place rose liquid distilled from honeysuckle flowers or lotus leaves 12#, the storage rose liquid distilled from honeysuckle flowers or lotus leaves 11# of 2 years of 1 year.
2. bacteriostatic test
The yellow bright aspergillus of indicator bacteria and the aspergillus niger selected carry out rejuvenation cultivation, and its cultivation, experimental procedure are with embodiment 1.The bacteriostatic diameter D recording is:
The bacteriostatic diameter of 6# rose liquid distilled from honeysuckle flowers or lotus leaves: yellow bright aspergillus=32mm, aspergillus niger D=18.5mm;
The bacteriostatic diameter of 11# rose liquid distilled from honeysuckle flowers or lotus leaves: yellow bright aspergillus D=12.5mm, aspergillus niger D=10mm;
The bacteriostatic diameter of 12# rose liquid distilled from honeysuckle flowers or lotus leaves: yellow bright aspergillus D=28mm, black-koji mould D=10mm;
3. the oral rose flavored juice that rose is prepared into
Get rose liquid distilled from honeysuckle flowers or lotus leaves 100ml and the rock sugar heating that ultrasonic extraction produces and be taken in small doses at short intervals, control tuberculosis cough and spit blood.This oral rose flavored juice, except having pleasant fragrance, is also rich in hundreds of nutritional labelings that are of value to health, can and blood, nourishing the stomach, moistening lung, wide chest loose strongly fragrant, have a respite and invigorate blood circulation, beautifying face and moistering lotion, make us refreshing and feel well.For tea, be taken in small doses at short intervals and can control stomachache due to emotional depression and the hyperactive liver-qi attacking the stomach, she also has certain antibacterial bacteriostatic ease constipation liver-protecting function simultaneously.
Embodiment 3
The rose liquid distilled from honeysuckle flowers or lotus leaves that adopts microwave low temperature extractive distillation, can be prepared as medicine
Rose flavored juice produce as follows:
Take fresh-rose 1000g, amount of water is 20 times of flower, and the temperature of Microwave Extraction is 60 ℃, and microwave power is 150W, and extraction time is 25min.Rose, under Microwave Extraction technology, can make rapidly the cell membrane of rose penetrate, and intracellular all kinds of chemical composition discharges thereupon, then enters after steam distillation 2h, obtains rose liquid distilled from honeysuckle flowers or lotus leaves, and its main component is as shown in table 1.
After all fractions are mixed into one, place the rose liquid distilled from honeysuckle flowers or lotus leaves 6# of month, place rose liquid distilled from honeysuckle flowers or lotus leaves 12#, the storage rose liquid distilled from honeysuckle flowers or lotus leaves 11# of 2 years of 1 year.
2. antibacterial test
Indicator bacteria Gram-positive, the negative bacteria selected carry out rejuvenation cultivation, and its cultivation, experimental procedure are with embodiment 1.The bacteriostatic diameter D recording is:
The bacteriostatic diameter of 6# rose liquid distilled from honeysuckle flowers or lotus leaves: yellow bright aspergillus D=32mm, the mould D=18.5mm in aspergillus niger=18.5:
The bacteriostatic diameter of 11# rose liquid distilled from honeysuckle flowers or lotus leaves: yellow bright aspergillus D=12.5mm, aspergillus niger D=10mm;
The bacteriostatic diameter of 12# rose liquid distilled from honeysuckle flowers or lotus leaves: yellow bright aspergillus D=28mm, aspergillus niger D=10mm
3. rose liquid distilled from honeysuckle flowers or lotus leaves is prepared into the application of medicine
Get the rose liquid distilled from honeysuckle flowers or lotus leaves 50ml of microwave method distillation, safflower 5g, RADIX ANGELICAE SINEINSIS 5g,, heating is removed slag, seven doses of good wine kimonos.There is menstruction regulating and pain relieving effect, can also comprehensive adjusting female reproductive system function, strengthen Monitoring Ovarian Function; Regulate estrogen and lutern secretion, adjust the menstrual cycle, and reduce too much menses, calm premenstrual tension; Can activate blood circulation and disperse blood clots, restore menstrual flow and invigorate blood circulation, effectively improve dysmenorrhoea, irregular menses etc.Some chemical properties and the content of table 1 rose liquid distilled from honeysuckle flowers or lotus leaves
| Sequence number | Compound composition title | Content (μ g/mL) | Molecular weight |
| 1 | Citronellol | 496.1 | C 10H 20O |
| 2 | Geraniol | 111.9 | C 10H 18O |
| 3 | Linalool | 46.6 | C 10H 18O |
| 4 | Nerol | 307.5 | C 10H 18O |
| 5 | Benzyl carbinol | 38.4 | C 8H 10O |
| 6 | Note product alcohol | 6.7 | C 10H 18O |
| 7 | Geranial | 1.8 | C 10H 16O |
| 8 | Neral | 10.0 | C 10H 16O |
| 9 | Geraniol formate | 1.4 | C 11H 20O 2 |
| 10 | Geraniol acetate | 6.5 | C 12H 22O 2 |
| 11 | β-myrcene | 0.8 | C 10H 16 |
| 12 | Ethanol | 0.8 | C 2H 6O |
| 13 | Eugenol | 46.2 | C 10H 12O 2 |
| 14 | Citronellal | 1.2 | |
| 15 | Eugenol | 65.8 | C 10H 12O 2 |
| 16 | 2-undecyl ketone | 1.6 | C 11H 22O |
| 17 | 2-undecyl alcohol | 1.2 | C 11H 21O |
| 18 | Tridecanol | 1.0 | C 13H 28O |
| 19 | Docosane | 11.2 | C 22H 46 |
| 20 | Tricosane | 24.0 | C 23H 48 |
| 21 | Palmitic acid | 9.7 | C 18H 12O 2 |
| 22 | 9-octadecenoic acid | 2.9 | C 18H 31O 2 |
| 23 | Ten triketones | 78.4 | C 13H 26O |
| 24 | Pentacosane | 12.5 | C 25H 52 |
| 25 | Aristolone | 32.5 | C 15H 22O |
| 26 | Eugenol methyl ether | 31.0 | C 11H 11O 2 |
| 27 | Cis rose oxide | 7.7 | C 10H 18 |
| 28 | Trans rose oxides | 17.9 | C 10H 18 |
| 29 | Ethyl palmitate | 19.9 | C 18H 36O 2 |
| 30 | Fanesol | 41.8 | C 15H 26O |
Table 1 has been contained main composition and the part submember of rose liquid distilled from honeysuckle flowers or lotus leaves of producing.
Embodiment 4
Rose flavored juice produce as follows:
Get new fresh-rose 1000g, amount of water is 40 times of flower weight, and the rose liquid distilled from honeysuckle flowers or lotus leaves of producing after steam distillation 3h is carried out in heating, and its main component is as shown in table 1.
After all fractions are mixed into one, place the rose liquid distilled from honeysuckle flowers or lotus leaves 6# of month, place rose liquid distilled from honeysuckle flowers or lotus leaves 12#, the storage rose liquid distilled from honeysuckle flowers or lotus leaves 11# of 2 years of 1 year.
2. bacteriostatic test
Adopt dull and stereotyped scraps of paper determination method, detect the rose liquid distilled from honeysuckle flowers or lotus leaves that distillation is produced;
First prepare nutrient matrix flat board, the unpasteurized 1cm scraps of paper immersed respectively above-mentioned all kinds of rose dew retting to be measured after 10 minutes again, take out and with the 2-6 piece of scraps of paper, put into matrix flat board respectively, to immerse the not sterilizing scraps of paper of sterile purified water as contrast, insert respectively constant temperature culture, cultivation temperature 32-37 ℃, cultivates 5 days;
The preliminary test of rose liquid distilled from honeysuckle flowers or lotus leaves bacteriostasis.
Test result shows: the Ck-W-in Fig. 2 looks into: be the control treatment of Cha Shi culture medium, rose liquid-05-2-looks into: for what detect in Cha Shi culture medium, contrast has grown bacterium colony, and tested rose liquid distilled from honeysuckle flowers or lotus leaves 05-1 is without any colony growth, illustrate that rose liquid distilled from honeysuckle flowers or lotus leaves has suppressed the miscellaneous bacteria on the sterilizing scraps of paper not.W: for measuring the abbreviation of the sterilized water water that immerses of the scraps of paper
Detect the mushroom in control group, it is the preservations such as daily food, nutrient and healthcare products and accidentally often grows aspergillus mushroom:
Aspergillus flavus (Asperillus flaous), aspergillus oryzae (Asperillus oryzal), Aspergillus terreus (A.terreus), aspergillus nidulans (A.hidulcms), aspergillus fumigatus (A.famigatus) aspergillus niger (A.niger) or yellow bright aspergillus (A fiavipes).
This flora of aspergillus especially in soil and air common particularly in moist season this class mould grow more prevailing, they are that beneficial microbe can be harmful to again under certain conditions to above-mentioned these bacterium under certain conditions, so the article of depositing are at normal temperatures subject to their infringement sometimes, but under normal temperature, rose liquid distilled from honeysuckle flowers or lotus leaves just has the action effect that suppresses this class bacterium.Simultaneously we to retrieve again rose volatile oil inhibited to the fungi candida albicans of human body deep infection, but the bacteriostasis of Eurotium be yet there are no to report, and the bacteriostasis of rose liquid distilled from honeysuckle flowers or lotus leaves is also without seeing foot.The present invention is to the one innovation in the application of rose liquid distilled from honeysuckle flowers or lotus leaves.By our research, find that the chemical composition in rose liquid distilled from honeysuckle flowers or lotus leaves acts synergistically each other, complete bactericidal action.Therefore, the mixture of rose liquid distilled from honeysuckle flowers or lotus leaves is playing a part bacteriostasis but not certain composition.
Claims (1)
1. the application of an Aspergillus bacteriostatic agent, it is characterized in that: described bacteriostatic agent can be used as there are drink, the food additives of bacteriostasis, health products, medicine, cleaning agent or the fruit of integration of drinking and medicinal herbs dredged antistaling agent, its active ingredient of described bacteriostatic agent is rose wate extract, and rose wate extract is inhibited to Aspergillus;
Described Aspergillus is aspergillus flavus, aspergillus oryzae, Aspergillus terreus, aspergillus nidulans or the aspergillus fumigatus in Eurotium;
The extracting method of described rose wate extract is steam distillation, ultrasonic extraction or Microwave Extraction method, the rose wate extract obtaining also can be referred to as rose liquid distilled from honeysuckle flowers or lotus leaves, and it is spent through the rose elite of extracting, all active ingredients of attar of rose are produced and retained in distillation entirely by rose;
Described steam distillation is, gets rose, with extraordinarily water of solid-liquid ratio 1:20～40, adds thermal distillation 2～5 hours, obtains rose wate extract;
Described ultrasonic extraction is, get rose with solid-liquid ratio 1:15～30 extraordinarily water carry out ultrasonic extraction, operating frequency 20～40KHz, electric power 100～400W, temperature 30～80 degree, 10～45 minutes time, and then by steam distillation 2～3 hours, obtain rose wate extract;
Described Microwave Extraction method is, gets rose with extraordinarily water of solid-liquid ratio 1:15～20, microwave power 100～400W, and temperature 30～80 degree, 20～50 minutes time, and then with steam distillation 2～3 hours, acquisition rose wate extract.
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| CN102551134B (en) * | 2011-09-13 | 2014-05-28 | 宗欣生态科技(上海)有限公司 | Rose extract liquid beverage and preparation method thereof |
| CN102406209B (en) * | 2011-11-28 | 2013-04-10 | 新疆和田阳光沙漠玫瑰有限公司 | Liquid drink containing active ingredients of rose essential oil and preparation method for liquid drink |
| CN105662947A (en) * | 2016-01-20 | 2016-06-15 | 杨德刚 | Application and preparation method of plant essence |
| CN109481539A (en) * | 2018-12-28 | 2019-03-19 | 佛山科学技术学院 | A kind of dysmenorrhea plaster containing agilawood essential oil |
| CN113396978A (en) * | 2021-06-29 | 2021-09-17 | 宜宾学院 | Rose-flavored yogurt and preparation method thereof |
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| CN1070545A (en) * | 1992-09-29 | 1993-04-07 | 孙恩奎 | Sterilization, disinfectant and compound method thereof |
| CN1555847A (en) * | 2004-01-05 | 2004-12-22 | 王贵林 | Medicne for preventing and treating virus and its preparaton method |
| CN101019561A (en) * | 2007-03-14 | 2007-08-22 | 郑泰林 | Air germicide for antagonizing epidemic encephalitis and influenza viruses |
| JP2007246407A (en) * | 2006-03-14 | 2007-09-27 | Noevir Co Ltd | Anti-staphylococcus aureus agent |
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2007
- 2007-12-20 CN CN200710151182.2A patent/CN101461558B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1070545A (en) * | 1992-09-29 | 1993-04-07 | 孙恩奎 | Sterilization, disinfectant and compound method thereof |
| CN1555847A (en) * | 2004-01-05 | 2004-12-22 | 王贵林 | Medicne for preventing and treating virus and its preparaton method |
| JP2007246407A (en) * | 2006-03-14 | 2007-09-27 | Noevir Co Ltd | Anti-staphylococcus aureus agent |
| CN101019561A (en) * | 2007-03-14 | 2007-08-22 | 郑泰林 | Air germicide for antagonizing epidemic encephalitis and influenza viruses |
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| JP昭58-41804A 1983.03.11 |
| 具体实施方式. |
| 李晓晴 等.玫瑰挥发油抗念珠菌活性的体外研究及两种药敏试验方法的比较.《中国皮肤性病学杂志》.2006,第20卷(第6期),第321-323页. |
| 玫瑰挥发油抗念珠菌活性的体外研究及两种药敏试验方法的比较;李晓晴 等;《中国皮肤性病学杂志》;20061231;第20卷(第6期);第321-323页 * |
| 龙刚.玫瑰水提物的毒理学与抗衰老功效研究.《中国优秀博硕士学位论文全文数据库 (硕士)基础科学辑》.2005,(第5期),论文全文. * |
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| Publication number | Publication date |
|---|---|
| CN101461558A (en) | 2009-06-24 |
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