CN109652271A - A kind of production method of health care Spondias axillaris fruit wine - Google Patents
A kind of production method of health care Spondias axillaris fruit wine Download PDFInfo
- Publication number
- CN109652271A CN109652271A CN201910120402.8A CN201910120402A CN109652271A CN 109652271 A CN109652271 A CN 109652271A CN 201910120402 A CN201910120402 A CN 201910120402A CN 109652271 A CN109652271 A CN 109652271A
- Authority
- CN
- China
- Prior art keywords
- fruit
- fruit stone
- fruit wine
- stone
- fermentation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
- C12G3/00—Preparation of other alcoholic beverages
- C12G3/02—Preparation of other alcoholic beverages by fermentation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/11—Pteridophyta or Filicophyta (ferns)
- A61K36/12—Filicopsida or Pteridopsida
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/72—Rhamnaceae (Buckthorn family), e.g. buckthorn, chewstick or umbrella-tree
- A61K36/725—Ziziphus, e.g. jujube
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/14—Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/20—Hypnotics; Sedatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P27/00—Drugs for disorders of the senses
- A61P27/02—Ophthalmic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/06—Antihyperlipidemics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/12—Antihypertensives
Abstract
The invention discloses a kind of production methods of health care Spondias axillaris fruit wine, include the following steps: the processing of (1) fresh wild jujube fruit；(2) it ferments；(3) fruit stone is handled: addition enzymolysis liquid enzymatic hydrolysis is placed in extractant after fruit stone is carried out Crushing of Ultrafine, obtains extraction solution using microwave auxiliary extraction；Fruit stone extracted is placed in supercritical extraction reactor, supercritical carbon dioxide extracting is carried out and obtains fruit stone essential oil；The extractant petroleum ether, chloroform, ethyl acetate, n-butanol, ethyl alcohol, water mixing composition；(4) ferment: fruit wine, fruit stone essential oil, anethole, hop acid, cloves alcohol extract, the ginkgo alcohol extracts from the leaves that extraction solution and step (2) are obtained, which mix, to be stood, and is made fermentation kind with pure rice wine, is again sealed off fermentation；(5) it filters, clarify.The present invention is sterilized before fermentation using natural plants bacteriostatic agent, and sterilization effect is good, while extracting fruit stone active constituent using the high extractant of dissolution extraction effect, improves the utility value of fruit stone.
Description
Technical field
The present invention relates to the production method technical fields of fruit wine, and in particular to a kind of production side of health care Spondias axillaris fruit wine
Method.
Background technique
Spondias axillaris is the fruit (fresh) or fruit stone of Anacardiaceae plant Spondias axillaris also known as five fruits.It is high for wild deciduous tree
8-20m；Trunk is straight and upright, and bark taupe, lobe is peeled off in the form of sheets, and sprig is sturdy, and dark violet brown is hairless, has hole skin.Odd number plumage
Shape compound leaf alternate, long 25-40cm, the long 3-5mm of petiolule；7-15 pieces of leaflet, to life, film quality to papery, ovate-elliptic or length
Ellipse, long 4-12cm, wide 2-5cm, long tapering, the base portion deflection of apex shape of tail, full edge, two sides is hairless or dilute blade back arteries and veins quilt
Hair；8-10 pairs of lateral vein.Flower polygamy, different strain；Male flower and pseudohermaphrodite flower lilac red are arranged in the sympodium circular cone of basidixed or axillary
Inflorescence, long 4-10cm；Female flower list is born in the axil of top；Sepal, petal each 5；Stamen 10；Ovary Room 5；Style 5 separates, long
About 0.5mm.Drupe ellipse or obovate, long 2-3cm, diameter about 2cm, yellow when mature, middle pericarp meat pulpous state, sweet and sour,
Fruit stone long 2-2.5cm, diameter 1.2-1.5cm, apex have 5 apertures.April at florescence, the fruiting period 8-10 month.Be distributed mainly on Zhejiang, Fujian,
Hubei, Hunan, Guangdong, Guangxi, Yunnan, Guizhou etc..Spondias axillaris fruit annual output is huge, and due to tart flavour weight, few people are edible,
It wastes every year countless.
It is pointed out in Compendium of Material Medica, Spondias axillaris acid sweet in flavor is mild-natured, nontoxic；Control trusted subordinate's fever and chills, evil knot gas gathers, has aches in the limbs
Arthritis with fixed pain caused by dampness；Long term usage pacifies beneficial the five internal organs, macrobiosis of making light of one's life by commiting suicide；Can control it is vexatious must not sleep, navel up and down pain, blood turn to let out long, the diseases such as abnormal sweating polydipsia；Bowl spares
Beneficial irritability, strengthens the bone, and helps negative gas, and it is strong to make one fertilizer.Thought according to modern medicine study: Spondias axillaris fruit is containing sugar, fat, albumen
A variety of nutritions and coumarin kind compound, phytosterol, saponin etc. such as matter, a variety of amino acid, silicon, calcium, potassium, sodium are effective
Pharmaceutical compositions.With beautifying face and moistering lotion, blood fat-reducing blood pressure-decreasing, reduce the functional healths effects such as cholesterol, promotion appetite, raising immunity；
According to related results of animal show Spondias axillaris prerun fruit containing the Flavones of Choerospondias Axillaris Fructus in coumarin kind compound mainly have with
Lower pharmacological action: 1, to animal resist oxygen lack and acute myocardial ischemia there is conservation effect；2, antiarrhythmic effect；3, to ADP
The rabbit platelet aggregation of (adenosine diphosphate (ADP)) induction has obvious inhibiting effect.
The invention of Spondias axillaris fruit wine, it is non-specific for having other than its special mouthfeel is to a kind of new enjoyment of consumer
Property cough, chronic prostatitis, the not good enough middle-aged and the old that sleeps has a better effect, can also making full use of out to wild fruit
Open up wide prospect.
Existing Spondias axillaris Processing Technology of Fruit Wine is less to the research and utilization of fruit stone, it is common practice that after fruit stone is boiled,
It ferments being mixed after water cooling with primary fermentation object again, some active materials in fruit stone are soluble in water, and fruit stone is further utilized
Active constituent or fruit stone is directly soaked in high wine, but the method fruit stone is not fully utilized, fruit stone
Utilization rate it is not high；And the sterilization process for removing miscellaneous bacteria is generally omitted in existing Processing Technology of Fruit Wine, or use sulfur dioxide
Sterilizing, but sulfur dioxide can reduce the color of fruit wine, while sulfur dioxide can also make fruit wine generation more difficult in conjunction with pigment
The smell of news influences the flavor of fruit wine, and sulfur dioxide usage amount control is improper, and fermentation can be made to terminate in advance, influence to ferment.
Summary of the invention
The present invention overcomes in the prior art, Spondias axillaris fruit stone utilization rate is low, and sulfur dioxide is used in fruit wine manufacture craft
The technical issues of fruit wine weak flavor made of sterilizing, provide a kind of production method of health care Spondias axillaris fruit wine.
To solve the above problems, the present invention adopts the following technical scheme that:
A kind of production method of health care Spondias axillaris fruit wine, includes the following steps:
(1) it the processing of fresh wild jujube fruit: selects fruit fresh, mature, that nothing is rotten and airing is eluted with water；
(2) it ferments: fruit is removed the peel, the water sterilized, bronze bell wood flower, fruit, rock sugar are mixed and be distributed into vial, is sealed
Fermentation；It was shaken 1 time every 10 days, is sufficiently mixed water, bronze bell wood flower, fruit, rock sugar；It all floats after 3 months to pulp, aqueous
Become clear, breakdown sealing is put into another container using siphon principle suction fruit wine and is stored；Fruit stone in former bottle with meat is grabbed to squeeze and is taken
The juice of fruit stone out, extrusion stores together with fruit wine；
(3) fruit stone is handled: 2~5h of addition enzymolysis liquid enzymatic hydrolysis is placed in extractant after fruit stone is carried out Crushing of Ultrafine, is used
Obtain extraction solution microwave auxiliary extraction 2~3 times；Fruit stone extracted is placed in supercritical extraction reactor, carries out overcritical two
Fruit stone essential oil is obtained by extraction in carbonoxide；The extractant is 1~2:2~4:5~7:6~8:6~8:10 petroleum by mass ratio
Ether, chloroform, ethyl acetate, n-butanol, ethyl alcohol, water mixing composition；The mixed proportion of the fruit stone and extractant is 10g:20ml；
(4) it ferments: fruit wine, fruit stone essential oil, anethole, the hop acid, cloves alcohol extracting that extraction solution and step (2) are obtained
Liquid, ginkgo alcohol extracts from the leaves, which mix, stands 2~3h, and adds 25 degree of pure rice wines to make fermentation kind with the volume ratio of 10:0.1~0.5, then
25~35d of secondary sealing and fermenting obtains fermentation liquid；
(5) it filters, clarify: fermentation liquid is stood, after fermentation liquid is pure and impure clearly, then with 50 degree of pure rice wines with 1~3:5's
Mass ratio and fermentation liquid mix, then add sea-buckthorn and Kiwi berry pulp is again sealed off 25~35d of fermentation and obtains mixed fermentation liquid, to
Mixed fermentation liquid upper layer becomes clear, seals filtering, packing, sealing under vacuum conditions with double layer disinfecting gauze, packaging is protected
Strong Spondias axillaris fruit wine finished product；The alcoholic strength of the Spondias axillaris fruit wine is 20~22 degree.
Wherein, in step (4), fruit wine that the extraction solution, step (2) obtain, fruit stone essential oil, anethole, hop acid,
Cloves alcohol extract, ginkgo alcohol extracts from the leaves mass ratio be 0.1~1:100:0.1~1:0.1~0.5:0.1~1:0.1~1:
0.05~0.1.
Wherein, in step (1), the water, bronze bell wood flower, fruit, rock sugar mass ratio be 7~10:0.1~1:1~3:2
~5.
Wherein, in step (3), the fruit stone essential oil is prepared by the following method: fruit stone extracted is placed in super face
It is 25kpa, extraction temperature in extracting pressure using the chloroform of equal mass ratioes, petroleum ether, ethyl acetate as entrainer in boundary's extraction kettle
Degree is 25~30 DEG C, and 20~30min of extraction obtains the fruit stone essential oil under conditions of carbon dioxide flow rate is 25L/min.
Wherein, the enzymolysis liquid is grouped as by following group: laccase, cellulase, beta amylase, phosphorylase.Wherein,
The enzyme activity of laccase is not less than 2000U/g, and cellulose enzyme vigor is not less than 3000U/g, and beta amylase enzyme activity is not less than
200U/g, phosphorylase enzyme activity are not less than 1000U/g.
Wherein, in step (5), the additive amount of the sea-buckthorn is the 20~30% of quality of fermentation broth, the Kiwi berry pulp
Additive amount is the 20~30% of quality of fermentation broth.
Wherein, in step (3), the microwave power of the microwave abstracting is 500W, and the microwave abstracting time is 5~15min.
The present invention has the advantages that compared with prior art
(1) fruit stone active material dissolution rate of the present invention is high, and fruit stone utility value is high.The present invention by Spondias axillaris fruit stone carry out for
It adds enzymolysis liquid after crushing to be digested, in enzymolysis liquid, laccase, cellulase, beta amylase, phosphorylase cooperation can cooperate with work
With by cellulose, lignin, the hemicellulose degradation in fruit stone, breaks plant cell wall, on the one hand promote active matter in fruit stone
The dissolution of matter, one side degrading enzyme have the function of digesting the intermediate product degraded in fruit stone at polysaccharide, in rear supervention
In ferment technique, it is further fermented into polyalcohol, improves the utility value of Spondias axillaris fruit stone.Extractant selection using upper,
The prior art mainly uses water or ethanol water as extractant, since the polarity of water is big, is easy to protein, carbohydrate etc.
Ingredient soluble easily in water is dissolved out, but free Flavonoids are insoluble in water, and the liposoluble substances such as flavones, alkaloid can be preferable
It is dissolved in the weaker substance of ethyl alcohol isopolarity, and the present invention is using petroleum ether, chloroform, ethyl acetate, n-butanol, ethyl alcohol, water composition
Extractant extract Spondias axillaris fruit stone, in extractant composition, the polar bond with opposed polarity power, to the extraction of substance selection
Property is wide, can preferably come out the substance of opposed polarity in fruit stone, such as the preferable dissolution extraction of flavones, alkaloid, polysaccharide, mention
The dissolution rate of active material in high fruit stone.
(2) fruit stone essential oil, anethole, hop acid, cloves alcohol extract, ginkgo leaf alcohol extracting are used in sterilization process of the invention
The microbial bacteria in fruit wine that object collaboration sterilizing obtains extraction solution and step (2) inhibits to kill.Mainly contain in fruit stone essential oil
Have a flavonoids and polyphenolic substance, it is single using fruit stone essential oil when, sterilization effect is affected by fruit stone essential oil concentration, need to compared with
Big concentration fruit stone essential oil can be only achieved preferable sterilization effect, and hop acid is mobile ionophore, can eliminate cross-film pH
Gradient reduces the pH value of microbial bacteria intracellular fluid, breaks the homeostasis of bacterium, promote inactivation of bacteria, can mainly inhibit
The growth of lactic acid bacteria, but the growth of saccharomycete cannot be inhibited, it is antibacterial single.Cloves alcohol extract mainly contains eugenol, cloves
Polar group on phenol can destroy normal metabolic function in conjunction with the enzymatic activity group of microorganism, influence the growth of microorganism,
When single use cloves alcohol extract, sterilization effect is also influenced by cloves alcohol extract concentration, and sterilization effect is poor.In ginkgo alcohol extracts from the leaves
Containing flavonoids, terpene, lactone, phenolic acid class and alkaloid isoreactivity substance, fungistatic effect is strong, but can inhibit includes ferment
Various bacteria including female bacterium adds the progress that excessive ginkgo alcohol extracts from the leaves is unfavorable for subsequent fermentation technique.And the present invention adopts
Cooperated with anethole, hop acid, cloves alcohol extract, ginkgo alcohol extracts from the leaves and fruit stone essential oil, sterilizing can be played to a variety of strains
Effect, sterilizing range is wide, can kill multiple microbial strains under lower usage amount, sterilization effect is good, while not influencing fruit wine
Flavor and taste, do not influence the progress of subsequent fermentation technique.
(3) fruit wine finished product made of the present invention can containing a variety of sterilizing bacteriostatic active ingredients such as flavonoids, Polyphenols
The growth for effectively inhibiting microorganism would be even more beneficial to the storage of product long period.
(4) fruit wine of the present invention has invigorating spleen and reinforcing stomach, brain nourishing and eyesight improving, calm tranquilizing the mind, strengthening the muscles and bones, beautifying face and moistering lotion, lipid-loweringing drop
Pressure, promotion appetite, raising immunity, reduction cholesterol and other effects, it is especially good to the protecting effect of heart, the rhythm of the heart can be stablized.
Spondias axillaris fruit wine healthcare active content of the invention is high, if plant polyose, protein, polyphenol compound equal size are high,
Fruit stone essential oil contains a variety of fatty acid such as myristic acid, palmitinic acid, palmitoleic acid, stearic acid, oleic acid, linoleic acid, Kiwi berry, sand
Vitamin content is high in spine, and especially the content of vitamin C and vitamin E is high, bronze bell wood spend in contain Co-Q10.This is several
The mutual cooperation of raw material, full of nutrition, plurality kinds of health care ingredient synergistic effect are good to the protecting effect of heart.
Specific embodiment
Below with reference to embodiment and experiment, the invention will be further described.
Embodiment 1
A kind of production method of health care Spondias axillaris fruit wine, includes the following steps:
(1) it the processing of fresh wild jujube fruit: selects fruit fresh, mature, that nothing is rotten and airing is eluted with water；
(2) it ferments: fruit is removed the peel, the water sterilized, bronze bell wood flower, fruit, rock sugar are mixed and be distributed into vial, is sealed
Fermentation；It was shaken 1 time every 10 days, is sufficiently mixed water, bronze bell wood flower, fruit, rock sugar；It all floats after 3 months to pulp, aqueous
Become clear, breakdown sealing is put into another container using siphon principle suction fruit wine and is stored；Fruit stone in former bottle with meat is grabbed to squeeze and is taken
The juice of fruit stone out, extrusion stores together with fruit wine；The water, bronze bell wood flower, fruit, rock sugar mass ratio be 10:0.1:3:
2。
(3) fruit stone is handled: addition is by laccase, cellulase, beta amylase, phosphorylase group after fruit stone is carried out Crushing of Ultrafine
At enzymolysis liquid enzymatic hydrolysis 5h be placed on by mass ratio be 1 :~4:5:8:6:10 petroleum ether, chloroform, ethyl acetate, n-butanol,
In the extractant that ethyl alcohol, water mixing form, extraction solution is obtained using microwave auxiliary extraction 2~3 times；Fruit stone extracted is set
In supercritical extraction reactor, carries out supercritical carbon dioxide extracting and obtain fruit stone essential oil；The mixing ratio of the fruit stone and extractant
Example is 10g:20ml；The microwave power of the microwave abstracting is 500W, and the microwave abstracting time is 15min；
(4) it ferments: fruit wine, fruit stone essential oil, anethole, the hop acid, cloves alcohol extracting that extraction solution and step (2) are obtained
Liquid, ginkgo alcohol extracts from the leaves, which mix, stands 3h, and adds 25 degree of pure rice wines to make fermentation kind with the volume ratio of 10:0.1, is again sealed off hair
Ferment 35d obtains fermentation liquid；Fruit wine, fruit stone essential oil, anethole, the hop acid, syringyl alcohol that the extraction solution, step (2) obtain
Extract, ginkgo alcohol extracts from the leaves mass ratio be 0.1:100:1:0.1:1:0.1:0.01.
(5) it filters, clarify: fermentation liquid is stood, after fermentation liquid is pure and impure clearly, then with 50 degree of pure rice wines with the matter of 1:5
Amount than and fermentation liquid mix, then add sea-buckthorn and Kiwi berry pulp is again sealed off fermentation 35d and obtains mixed fermentation liquid, hair to be mixed
Zymotic fluid upper layer becomes clear, seals filtering, packing, sealing under vacuum conditions with double layer disinfecting gauze, packaging obtains health care south acid
Jujube fruit wine finished product；The alcoholic strength of the Spondias axillaris fruit wine is 20 degree.The additive amount of the sea-buckthorn is the 30% of quality of fermentation broth, institute
State 20% that Kiwi berry pulp additive amount is quality of fermentation broth.
In step (3), the fruit stone essential oil is prepared by the following method: fruit stone extracted is placed in supercritical extract
It is 25kpa, extraction temperature 30 in extracting pressure using the chloroform of equal mass ratioes, petroleum ether, ethyl acetate as entrainer in kettle
DEG C, extraction 20min obtains the fruit stone essential oil under conditions of carbon dioxide flow rate is 25L/min.
Embodiment 2
A kind of production method of health care Spondias axillaris fruit wine, includes the following steps:
(1) it the processing of fresh wild jujube fruit: selects fruit fresh, mature, that nothing is rotten and airing is eluted with water；
(2) it ferments: fruit is removed the peel, the water sterilized, bronze bell wood flower, fruit, rock sugar are mixed and be distributed into vial, is sealed
Fermentation；It was shaken 1 time every 10 days, is sufficiently mixed water, bronze bell wood flower, fruit, rock sugar；It all floats after 3 months to pulp, aqueous
Become clear, breakdown sealing is put into another container using siphon principle suction fruit wine and is stored；Fruit stone in former bottle with meat is grabbed to squeeze and is taken
The juice of fruit stone out, extrusion stores together with fruit wine；The water, bronze bell wood flower, fruit, rock sugar mass ratio be 7:1:1:5.
(3) fruit stone is handled: addition is by laccase, cellulase, beta amylase, phosphorylase group after fruit stone is carried out Crushing of Ultrafine
At enzymolysis liquid enzymatic hydrolysis 2h be placed on by mass ratio be 2:2:7:6:8:10 petroleum ether, chloroform, ethyl acetate, n-butanol, second
In the extractant that alcohol, water mixing form, extraction solution is obtained using microwave auxiliary extraction 2 times；Fruit stone extracted is placed in super
In critical extraction kettle, carries out supercritical carbon dioxide extracting and obtain fruit stone essential oil；The mixed proportion of the fruit stone and extractant is
10g:20ml；The microwave power of the microwave abstracting is 500W, and the microwave abstracting time is 15min；
(4) it ferments: fruit wine, fruit stone essential oil, anethole, the hop acid, cloves alcohol extracting that extraction solution and step (2) are obtained
Liquid, ginkgo alcohol extracts from the leaves, which mix, stands 2h, and adds 25 degree of pure rice wines to make fermentation kind with the volume ratio of 10:0.1~0.5, again
It is sealed by fermentation 25d and obtains fermentation liquid；Fruit wine that the extraction solution, step (2) obtain, fruit stone essential oil, anethole, hop acid,
Cloves alcohol extract, ginkgo alcohol extracts from the leaves mass ratio be 1:100:0.1:0.5:0.1:1:0.1.
(5) it filters, clarify: fermentation liquid is stood, after fermentation liquid is pure and impure clearly, then with 50 degree of pure rice wines with the matter of 3:5
Amount than and fermentation liquid mix, then add sea-buckthorn and Kiwi berry pulp is again sealed off fermentation 25d and obtains mixed fermentation liquid, hair to be mixed
Zymotic fluid upper layer becomes clear, seals filtering, packing, sealing under vacuum conditions with double layer disinfecting gauze, packaging obtains health care south acid
Jujube fruit wine finished product；The alcoholic strength of the Spondias axillaris fruit wine is 22 degree.The additive amount of the sea-buckthorn is the 20% of quality of fermentation broth, institute
State 30% that Kiwi berry pulp additive amount is quality of fermentation broth.
In step (3), the fruit stone essential oil is prepared by the following method: fruit stone extracted is placed in supercritical extract
It is 25kpa, extraction temperature 25 in extracting pressure using the chloroform of equal mass ratioes, petroleum ether, ethyl acetate as entrainer in kettle
DEG C, extraction 30min obtains the fruit stone essential oil under conditions of carbon dioxide flow rate is 25L/min.
Embodiment 3
A kind of production method of health care Spondias axillaris fruit wine, includes the following steps:
(1) it the processing of fresh wild jujube fruit: selects fruit fresh, mature, that nothing is rotten and airing is eluted with water；
(2) it ferments: fruit is removed the peel, the water sterilized, bronze bell wood flower, fruit, rock sugar are mixed and be distributed into vial, is sealed
Fermentation；It was shaken 1 time every 10 days, is sufficiently mixed water, bronze bell wood flower, fruit, rock sugar；It all floats after 3 months to pulp, aqueous
Become clear, breakdown sealing is put into another container using siphon principle suction fruit wine and is stored；Fruit stone in former bottle with meat is grabbed to squeeze and is taken
The juice of fruit stone out, extrusion stores together with fruit wine；The water, bronze bell wood flower, fruit, rock sugar mass ratio be 8:0.5:2:3.
(3) fruit stone is handled: addition is by laccase, cellulase, beta amylase, phosphorylase group after fruit stone is carried out Crushing of Ultrafine
At enzymolysis liquid enzymatic hydrolysis 4h be placed on by mass ratio be 2:3:6:7:7:10 petroleum ether, chloroform, ethyl acetate, n-butanol, second
In the extractant that alcohol, water mixing form, extraction solution is obtained using microwave auxiliary extraction 3 times；Fruit stone extracted is placed in super
In critical extraction kettle, carries out supercritical carbon dioxide extracting and obtain fruit stone essential oil；The mixed proportion of the fruit stone and extractant is
10g:20ml；The microwave power of the microwave abstracting is 500W, and the microwave abstracting time is 10min；
(4) it ferments: fruit wine, fruit stone essential oil, anethole, the hop acid, cloves alcohol extracting that extraction solution and step (2) are obtained
Liquid, ginkgo alcohol extracts from the leaves, which mix, stands 3h, and adds 25 degree of pure rice wines to make fermentation kind with the volume ratio of 10:0.3, is again sealed off hair
Ferment 30d obtains fermentation liquid；Fruit wine, fruit stone essential oil, anethole, the hop acid, syringyl alcohol that the extraction solution, step (2) obtain
Extract, ginkgo alcohol extracts from the leaves mass ratio be 0.5:100:0.5:0.3:0.5:0.5:0.05.
(5) it filters, clarify: fermentation liquid is stood, after fermentation liquid is pure and impure clearly, then with 50 degree of pure rice wines with the matter of 2:5
Amount than and fermentation liquid mix, then add sea-buckthorn and Kiwi berry pulp is again sealed off fermentation 30d and obtains mixed fermentation liquid, hair to be mixed
Zymotic fluid upper layer becomes clear, seals filtering, packing, sealing under vacuum conditions with double layer disinfecting gauze, packaging obtains health care south acid
Jujube fruit wine finished product；The alcoholic strength of the Spondias axillaris fruit wine is 21 degree.The additive amount of the sea-buckthorn is the 25% of quality of fermentation broth, institute
State 25% that Kiwi berry pulp additive amount is quality of fermentation broth.
In step (3), the fruit stone essential oil is prepared by the following method: fruit stone extracted is placed in supercritical extract
It is 25kpa, extraction temperature 28 in extracting pressure using the chloroform of equal mass ratioes, petroleum ether, ethyl acetate as entrainer in kettle
DEG C, extraction 25min obtains the fruit stone essential oil under conditions of carbon dioxide flow rate is 25L/min.
In order to illustrate technical effect of the invention, following control group is set:
Control group 1
Control group 1 and the production method of the Spondias axillaris fruit wine of embodiment 1 are essentially identical, and difference is, the extraction of control group 1
Agent is petroleum ether.
Control group 2
Control group 2 and the production method of the Spondias axillaris fruit wine of embodiment 1 are essentially identical, and difference is, the extraction of control group 2
Agent is chloroform.
Control group 3
Control group 3 and the production method of the Spondias axillaris fruit wine of embodiment 1 are essentially identical, and difference is, the extraction of control group 3
Agent is ethyl acetate.
Control group 4
Control group 4 and the production method of the Spondias axillaris fruit wine of embodiment 1 are essentially identical, and difference is, the extraction of control group 4
Agent is n-butanol.
Control group 5
Control group 5 and the production method of the Spondias axillaris fruit wine of embodiment 1 are essentially identical, and difference is, the extraction of control group 5
Agent is the ethanol solution that mass percentage is 80%.
Control group 6
Control group 6 and the production method of the Spondias axillaris fruit wine of embodiment 1 are essentially identical, and difference is, the enzymatic hydrolysis of control group 6
Liquid is only made of laccase.
Control group 7
Control group 7 and the production method of the Spondias axillaris fruit wine of embodiment 1 are essentially identical, and difference is, the enzymatic hydrolysis of control group 7
Liquid is only made of cellulase.
Control group 8
Control group 8 and the production method of the Spondias axillaris fruit wine of embodiment 1 are essentially identical, and difference is, the enzymatic hydrolysis of control group 8
Liquid is only made of beta amylase.
Control group 9
Control group 9 and the production method of the Spondias axillaris fruit wine of embodiment 1 are essentially identical, and difference is, the enzymatic hydrolysis of control group 9
Liquid is only made of phosphorylase.
Control group 10
Control group 10 and the production method of the Spondias axillaris fruit wine of embodiment 1 are essentially identical, and difference is, the step of control group 10
Suddenly in (4), fruit wine and fruit stone essential oil that extraction solution and step (2) obtain is mixed and carry out next step process again after standing.
Control group 11
Control group 11 and the production method of the Spondias axillaris fruit wine of embodiment 1 are essentially identical, and difference is, the step of control group 11
Suddenly in (4), fruit wine and anethole that extraction solution and step (2) obtain is mixed and carry out next step process again after standing.
Control group 12
Control group 12 and the production method of the Spondias axillaris fruit wine of embodiment 1 are essentially identical, and difference is, the step of control group 12
Suddenly in (4), fruit wine and hop acid that extraction solution and step (2) obtain is mixed and carry out next step process again after standing.
Control group 13
Control group 13 and the production method of the Spondias axillaris fruit wine of embodiment 1 are essentially identical, and difference is, the step of control group 13
Suddenly in (4), fruit wine and cloves alcohol extract that extraction solution and step (2) obtain is mixed and carry out next step process again after standing.
Control group 14
Control group 14 and the production method of the Spondias axillaris fruit wine of embodiment 1 are essentially identical, and difference is, the step of control group 14
Suddenly in (4), fruit wine and ginkgo alcohol extracts from the leaves that extraction solution and step (2) obtain is mixed and carry out next step work again after standing
Skill.
1, fruit stone dissolution rate detects
Using in 1~embodiment of high performance liquid chromatography detection embodiment 3 and 1~control group of control group 9 use extractant
Polysaccharide residual quantity in fruit stone extracted, with determined by ultraviolet spectrophotometry flavones, total phenol residual quantity, as a result such as the following table 1.
Table 1
As shown in Table 1, fruit stone polysaccharide residual quantity: 1~embodiment of embodiment, 3 5~control group of < control group, 9 < control group 1
~control group 4, fruit stone flavones residual quantity: 1~embodiment of embodiment, 3 6~control group of < control group, 7 1~control group of < control group 2
3 < control group of < control group, 4 < control group 5.Fruit stone total phenol residual quantity: 1~embodiment of embodiment, 3 6~control group of < control group 7
3 < control group of < control group 1~control group, 2 < control group, 4 < control group 5.Thus illustrate that the present invention is promoting fruit stone active matter
In the influence factor of matter dissolution rate, the influence of enzymolysis liquid is smaller, and the selection of extractant is affected, and it is single using it is a kind of at
It is different to polysaccharide, brass, the effect of extracting of phenolic compound in fruit stone when being allocated as extractant, and use petroleum ether, chlorine
When imitative, ethyl acetate, n-butanol, ethyl alcohol composition extractant extraction, coordinated, effect of extracting is best, active matter in fruit stone
Matter dissolution rate is high, and fruit stone utilization rate is big.
2, bactericidal effect
To in fruit wine preparation process in 1~embodiment of embodiment 3 and 10~control group of control group 14, step (4) is middle will
Fruit wine that extraction solution and step (2) obtain, fruit stone essential oil, anethole, hop acid, cloves alcohol extract, ginkgo alcohol extracts from the leaves are mixed
Total number of bacterial colonies (cfu/ml) such as the following table 2 in mixture after even standing.
Table 2
As shown in Table 2, the total plate count of bacterium is far below control group 10~control in the fruit wine of 1~embodiment of embodiment 3
Group 14 illustrates that the present invention plays collaboration using fruit stone essential oil, anethole, hop acid, cloves alcohol extract, the cooperation of ginkgo alcohol extracts from the leaves
The effect of synergy effectively can kill miscellaneous bacteria before fermentation, and sterilization effect is good.
Above description is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair
Bright patent claim, it is all the present invention suggested by technical spirit under completed same changes or modifications change, should all belong to
In the covered the scope of the patents of the present invention.
Claims (7)
1. a kind of production method of health care Spondias axillaris fruit wine, which comprises the steps of:
(1) it the processing of fresh wild jujube fruit: selects fruit fresh, mature, that nothing is rotten and airing is eluted with water；
(2) it ferments: fruit is removed the peel, the water sterilized, bronze bell wood flower, fruit, rock sugar are mixed and be distributed into vial, sealing hair
Ferment；It was shaken 1 time every 10 days, is sufficiently mixed water, bronze bell wood flower, fruit, rock sugar；It all floats after 3 months to pulp, aqueous becomes
Clearly, breakdown seals, and is put into another container and is stored using siphon principle suction fruit wine；Fruit stone in former bottle with meat is grabbed into crowded taking-up
The juice of fruit stone, extrusion stores together with fruit wine；
(3) fruit stone is handled: 2~5h of addition enzymolysis liquid enzymatic hydrolysis is placed in extractant after fruit stone is carried out Crushing of Ultrafine, using microwave
Obtain extraction solution auxiliary extraction 2~3 times；Fruit stone extracted is placed in supercritical extraction reactor, overcritical titanium dioxide is carried out
Fruit stone essential oil is obtained by extraction in carbon；The extractant is 1~2:2~4:5~7:6~8:6~8:10 petroleum ether, chlorine by mass ratio
Imitative, ethyl acetate, n-butanol, ethyl alcohol, water mixing composition；The mixed proportion of the fruit stone and extractant is 10g:20ml；
(4) ferment: fruit wine that extraction solution and step (2) are obtained, fruit stone essential oil, anethole, hop acid, cloves alcohol extract,
Ginkgo alcohol extracts from the leaves, which mixes, stands 2~3h, and adds 25 degree of pure rice wines to make fermentation kind with the volume ratio of 10:0.1~0.5, again
It is sealed by fermentation 25~35d and obtains fermentation liquid；
(5) it filters, clarify: fermentation liquid is stood, after fermentation liquid is pure and impure clearly, then with 50 degree of pure rice wines with the quality of 1~3:5
Than mixing with fermentation liquid, then add sea-buckthorn and Kiwi berry pulp is again sealed off 25~35d of fermentation and obtains mixed fermentation liquid, it is to be mixed
Fermentation liquid upper layer becomes clear, seals filtering, packing, sealing under vacuum conditions with double layer disinfecting gauze, packaging obtains health care south
Wild jujube fruit wine finished product；The alcoholic strength of the Spondias axillaris fruit wine is 20~22 degree.
2. a kind of production method of health care Spondias axillaris fruit wine according to claim 1, which is characterized in that in step (4), institute
State extraction solution, fruit wine that step (2) obtains, fruit stone essential oil, anethole, hop acid, cloves alcohol extract, ginkgo alcohol extracts from the leaves
Mass ratio is 0.1~1:100:0.1~1:0.1~0.5:0.1~1:0.1~1:0.05~0.1.
3. a kind of production method of health care Spondias axillaris fruit wine according to claim 1, which is characterized in that in step (1), institute
State water, bronze bell wood flower, fruit, rock sugar mass ratio be 7~10:0.1~1:1~3:2~5.
4. a kind of production method of health care Spondias axillaris fruit wine according to claim 1, which is characterized in that in step (3), institute
It states fruit stone essential oil to be prepared by the following method: fruit stone extracted is placed in supercritical extraction reactor, with the chlorine of equal mass ratioes
Imitative, petroleum ether, ethyl acetate are entrainer, are 25kpa in extracting pressure, extraction temperature is 25~30 DEG C, carbon dioxide flow rate
The fruit stone essential oil is obtained to extract 20~30min under conditions of 25L/min.
5. a kind of production method of health care Spondias axillaris fruit wine according to claim 1, which is characterized in that the enzymolysis liquid
It is grouped as by following group: laccase, cellulase, beta amylase, phosphorylase.
6. a kind of production method of health care Spondias axillaris fruit wine according to claim 1, which is characterized in that in step (5), institute
State sea-buckthorn additive amount be quality of fermentation broth 20~30%, the Kiwi berry pulp additive amount be quality of fermentation broth 20~
30%.
7. a kind of production method of health care Spondias axillaris fruit wine according to claim 1, which is characterized in that in step (3), institute
The microwave power for stating microwave abstracting is 500W, and the microwave abstracting time is 5~15min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910120402.8A CN109652271A (en) | 2019-02-18 | 2019-02-18 | A kind of production method of health care Spondias axillaris fruit wine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910120402.8A CN109652271A (en) | 2019-02-18 | 2019-02-18 | A kind of production method of health care Spondias axillaris fruit wine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109652271A true CN109652271A (en) | 2019-04-19 |
Family
ID=66123013
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910120402.8A Pending CN109652271A (en) | 2019-02-18 | 2019-02-18 | A kind of production method of health care Spondias axillaris fruit wine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109652271A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112779114A (en) * | 2021-03-29 | 2021-05-11 | 长江师范学院 | Preparation method of crispy plum fruit wine with active ingredients of ginkgo leaves and armillaria mellea |
| CN112980637A (en) * | 2019-12-17 | 2021-06-18 | 南京大学 | Preparation method of kiwi fruit-nitraria tangutorum bobr composite fruit wine |
| CN115232852A (en) * | 2022-09-07 | 2022-10-25 | 青海伊纳维康生物科技有限公司 | Preparation method of sea buckthorn oligopeptide with ACE (angiotensin converting enzyme) inhibitory activity |
| WO2023000440A1 (en) * | 2021-07-23 | 2023-01-26 | 海南华美益实业有限公司 | Morinda citrifolia compound wine having improved active ingredient content, and preparation method therefor |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104017691A (en) * | 2014-06-15 | 2014-09-03 | 罗中平 | Method for brewing schisandra chinensis fruit wine |
| CN104862172A (en) * | 2015-05-13 | 2015-08-26 | 中国中轻国际工程有限公司 | Production process of wild jujube wine |
| CN104862173A (en) * | 2015-05-13 | 2015-08-26 | 中国中轻国际工程有限公司 | Production process of wild jujube wine |
| CN105087235A (en) * | 2014-05-14 | 2015-11-25 | 王牌 | Process for making healthcare liquor from blackcurrant and gynostemma pentaphyllum |
| CN109197909A (en) * | 2018-08-22 | 2019-01-15 | 黑龙江省松花江林业管理局森林病虫害防治站 | A kind of bactericide and its preparation process |
-
2019
- 2019-02-18 CN CN201910120402.8A patent/CN109652271A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105087235A (en) * | 2014-05-14 | 2015-11-25 | 王牌 | Process for making healthcare liquor from blackcurrant and gynostemma pentaphyllum |
| CN104017691A (en) * | 2014-06-15 | 2014-09-03 | 罗中平 | Method for brewing schisandra chinensis fruit wine |
| CN104862172A (en) * | 2015-05-13 | 2015-08-26 | 中国中轻国际工程有限公司 | Production process of wild jujube wine |
| CN104862173A (en) * | 2015-05-13 | 2015-08-26 | 中国中轻国际工程有限公司 | Production process of wild jujube wine |
| CN109197909A (en) * | 2018-08-22 | 2019-01-15 | 黑龙江省松花江林业管理局森林病虫害防治站 | A kind of bactericide and its preparation process |
Non-Patent Citations (4)
| Title |
|---|
| 于新等: "《天然食品添加剂》", 31 March 2014, 中国轻工业出版社 * |
| 刘素纯等: "南酸枣酿酒酵母菌分离及南酸枣酒酿制研究", 《酿酒科技》 * |
| 向萍: "广枣核化学成分与广枣质量控制研究", 《中优秀硕士学位论文全文数据库 医药科技卫生辑》 * |
| 聂聪: "《酒花与啤酒酿造》", 31 October 2018, 中国轻工业出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112980637A (en) * | 2019-12-17 | 2021-06-18 | 南京大学 | Preparation method of kiwi fruit-nitraria tangutorum bobr composite fruit wine |
| CN112779114A (en) * | 2021-03-29 | 2021-05-11 | 长江师范学院 | Preparation method of crispy plum fruit wine with active ingredients of ginkgo leaves and armillaria mellea |
| WO2023000440A1 (en) * | 2021-07-23 | 2023-01-26 | 海南华美益实业有限公司 | Morinda citrifolia compound wine having improved active ingredient content, and preparation method therefor |
| CN115232852A (en) * | 2022-09-07 | 2022-10-25 | 青海伊纳维康生物科技有限公司 | Preparation method of sea buckthorn oligopeptide with ACE (angiotensin converting enzyme) inhibitory activity |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103966057B (en) | A kind of preparation method of Fragrant fruit wine | |
| CN109652271A (en) | A kind of production method of health care Spondias axillaris fruit wine | |
| CN105211880B (en) | Preparation method of fig enzymes | |
| CN103555487B (en) | Making process of compound red wine | |
| CN107518390A (en) | A kind of beautiful ferment of promise and preparation method thereof | |
| CN104643086A (en) | Preparation method of rose ferment | |
| CN104013947A (en) | Hair-restorer spraying liquid of fresh medicine, fruit and vegetable ferments with effects of preventing hair loss and restoring hair and preparation method thereof | |
| CN107691704A (en) | A kind of black-tea fungus drink and preparation method thereof | |
| CN107586682A (en) | A kind of brew method of alcoholic drink mixed with fruit juice | |
| KR101431417B1 (en) | The method of oriental composite for treatment of atopy and cancer | |
| CN104664507A (en) | Preparation method of pure natural pomegranate beverage | |
| KR20090098024A (en) | The method of manufacturing of medicinal wine | |
| CN103315359A (en) | Grifola frondosus solid state fermentation functional beverage and its preparation method | |
| KR20000055832A (en) | Preparation Method for Lactic Acid Bacterial Juice Using Vegetable and Oriental Medicine | |
| KR101716297B1 (en) | Method drinks for prevent a metabolic syndrome that made by Hericium erinaceus extracts containing crude drugs extracts. | |
| CN107236643A (en) | A kind of preparation method of Fragrant fruit wine | |
| KR20200094528A (en) | Healthful mixed tea development using fermented herbs, fruits and shiitake | |
| CN105695287A (en) | Selenium-rich healthcare black vinegar containing black fungus and mulberries | |
| CN106497722B (en) | Walnut flower pomegranate fruit wine and preparation method thereof | |
| CN109349483A (en) | A kind of plant energy functional beverage production method | |
| CN109567086A (en) | A kind of quick freezing mango puree and preparation method thereof | |
| CN109287991A (en) | A kind of blueberry puree and its preparation method and application | |
| CN107041445A (en) | A kind of bamboo juice leaf of bamboo health protection tea for preventing and treating hypertension, hyperglycemia | |
| CN102851164A (en) | Inonotus obliquus dry red wine | |
| KR102082820B1 (en) | Fermented vinegar beverage concentrates and manufacturing method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190419 |
|
| RJ01 | Rejection of invention patent application after publication |