CN105638641B - Application and its application method of Portugal's sweet dew glue in Saving specimen - Google Patents
Application and its application method of Portugal's sweet dew glue in Saving specimen Download PDFInfo
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- CN105638641B CN105638641B CN201610148749.XA CN201610148749A CN105638641B CN 105638641 B CN105638641 B CN 105638641B CN 201610148749 A CN201610148749 A CN 201610148749A CN 105638641 B CN105638641 B CN 105638641B
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- sample
- portugal
- sweet dew
- dew glue
- glue
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to application of Portugal's sweet dew glue in Saving specimen, the sample is zoological specimens or plant specimen；The invention further relates to a kind of store method of sample, the method is that after handling the sample 12~for 24 hours using formalin and/or ethanol solution, the sample is sealed up for safekeeping using Portugal's sweet dew glue gel；Portugal's sweet dew glue is applied in Saving specimen by the present invention for the first time, Portugal's sweet dew glue is during sample is preserved, the intact form of biological sample can be kept for a long time, there will not be the effects that any murder by poisoning or corrosion to sample, avoid the defects of sample body easily caused using solution such as formaldehyde, ethyl alcohol is hardened, deformed, faded, and the sample after preserving conveniently is moved, and is easy to transport；Portugal's sweet dew glue is applied to the preservation field of sample by the present invention, and a kind of new thinking is provided for Saving specimen field, has higher application value.
Description
Technical field
The present invention relates to biological sample preservation fields, and in particular to application and its use of Portugal's sweet dew glue in Saving specimen
Method.
Background technology
Biological sample is the important material of biological subject, is the important models of school instruction；And Science communication is important
Carrier.Biological sample determines the developing direction of following sample industry and wide distant view in the important function that every field plays.
Now, people increasingly pay attention to the making and preservation of biological sample.But for biology, method that people grasp at present
Preparation of specimen and the needs preserved far can not also be met, guarantor's color problem of especially biological sample is asking substantially of facing of people
Topic is also sixty-four dollar question.Because the riotous with colour of biology is key character existing for its bio-diversity, biology base is kept
The presence of this color is precious for the research of biological subject and biography, is all of great significance for the development of entire sample industry.
At present, fixed and preservation biological sample is most often using formalin and ethanol solution, but formaldehyde is molten
There are many defects in liquid, mainly have：（1）Formaldehyde easily makes sample body harden, deform, fade, is not fresh-keeping；（2）Formalin
It is middle easily to polymerize generation precipitation because containing methanol, it need to often replace；（3）Methanol has strong impulse smell, not only pollutes
Environment, the eyes and respiratory system of injury operator that can also be in various degree, seriously endangers health.And the preservation containing ethyl alcohol
Also sample body can be made to harden after liquid long-time service, discolouration phenomena becomes apparent from than formaldehyde；And the sample that both solution preserve
The defects of this presence is not easy moulding, transport trouble.
If it is possible to work out a kind of nontoxic pure natural medium suitable for preservation sample, will change
At present Saving specimen there are the problem of.
The content of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide application of Portugal's sweet dew glue in Saving specimen, Portugals
Sweet dew glue can keep the intact form of biological sample for a long time, will not have the effects that any murder by poisoning or corrosion to sample, avoid
The defects of sample body easily caused using solution such as formaldehyde, ethyl alcohol is hardened, deformed, faded.
Another object of the present invention is to provide a kind of store method of sample.
To achieve the above object, the present invention adopts the following technical scheme that：
Application of Portugal's sweet dew glue in Saving specimen, it is preferable that the sample is zoological specimens or plant specimen.
At present, the common store method of biological sample preservation field is that directly sample is placed in formalin to protect for a long time
It deposits, although formaldehyde is more common, formaldehyde preserves sample there are many defects, such as sample body can be made to harden, deform, take off
Color, it is not fresh-keeping the defects of, be not suitable for preserving sample for a long time.
It is a kind of microgranular edible glue of new multi-purpose that Portugal's sweet dew glue, which is also known as konjac glucomannan, be now widely used for food,
The fields such as beverage, medicine, daily-use chemical industry, scientific research, the substitute products as agar, pectin, algin etc.；Not yet someone will at present
Portugal's sweet dew glue is used for the preservation of sample, and inventor chances on during the store method of research sample, is killed if will pass through
Bacterium sterilizes is sealed up for safekeeping with the sample after fixing process using Portugal's sweet dew glue gel of certain concentration, can keep biological mark for a long time
This intact form, and avoid the sample body easily caused using solution such as formaldehyde, ethyl alcohol harden, deform, fading etc. it is scarce
It falls into, especially suitable for the long-term preservation to sample；In addition, Portugal's sweet dew glue is the pure natural production without any preservative and additive
Product will not have sample the effects that any murder by poisoning or corrosion, not interfere with the structure of sample body；And the Portugal's sweet dew used
Glue after water dissolution under room temperature for solid-state, can be good at keeping the form of sample, and be moved easily sample, the mark being prepared
Originally it is easy to transport, a kind of new thinking is provided for Saving specimen field, there is higher application value.
It is further preferable that the sample is aquatic animal.
A kind of store method of sample, the method are to handle the sample 12 using formalin and/or ethanol solution
~for 24 hours after, seal the sample up for safekeeping using Portugal's sweet dew glue gel.
Store method provided by the invention first carries out sample to be saved using formalin and/or ethanol solution
Processing, sterilization and fixed, pretreatment can be good at fixed preparation, avoid the later stage seal up for safekeeping during make when sterilizing not exclusively
Into sample rot the problems such as.
Preferably, the concentration of Portugal's sweet dew glue is 2~15%.
Inventor dissolves Portugal's sweet dew glue when Portugal's sweet dew glue is used to seal sample up for safekeeping, using warm water, by crowd
It is found after multiple creative experiments, not the dosage of Portugal's sweet dew glue of arbitrary proportion, which all has, preferably seals effect, and Portugal up for safekeeping
The concentration of sweet dew glue is nor its conventional concentration as edible glue；Serve as a model preservation Portugal's sweet dew glue concentration compared with
Greatly, within 2~15% concentration range, preservation effect is preferable；The concentration of Portugal's sweet dew glue is too low, and gel consistency is smaller, Yi Zao
Into the local deformation of sample, and the tension inside gel is inadequate, can not bear the sample of larger quality, and Portugal's sweet dew glue is dense
It spends greatly, then can significantly reduce the transparency of gel, influence the visuality of sample, although sample can be preserved, the external world is very
Difficulty observes sample by gel, loses the value and practicability of Saving specimen, and therefore, the concentration of Portugal's sweet dew glue is necessary
In the range of 2~15%.
Preferably, the concentration of Portugal's sweet dew glue is 3~6%.
Preferably, after handling the sample 12~for 24 hours using formalin and/or ethanol solution, using Portugal's sweet dew glue and
Carragheen is blended the compound adhesive to be formed and seals the sample up for safekeeping.During the experiment, inventor has found, if attempt using carragheen and
When the compound adhesive that Portugal's sweet dew glue blending is prepared preserves sample, in the viscosity and the condition of inner tensions for not reducing gel
Under, the transparency of gel is remarkably improved, increases the visuality of sample, two kinds of cementing conjunctions are more suitable for preserving sample.
Preferably, the mass ratio of Portugal's sweet dew glue and carragheen is 2~3:1；It is further preferable that Portugal's sweet dew glue and
The mass ratio of carragheen is 2:1.
In the present invention, time during to sample Biological Pretreatment is particularly important, and soaking time is too short, and sample biological fixation is not
Completely, and soaking time is too long, sample biology does not preserve the situation of colour fading of being just present with also；Preferably, using formalin and/
Or ethanol solution handles the 14~16h of sample.
Compared with prior art, the present invention has the advantages that：
Portugal's sweet dew glue is applied in Saving specimen by the present invention for the first time, and Portugal's sweet dew glue, can during sample is preserved
The long-term intact form for keeping biological sample will not have sample the effects that any murder by poisoning or corrosion, avoid using formaldehyde, second
The defects of sample body that the solution such as alcohol easily cause hardens, deforms, fading, and the sample after preservation is conveniently moved, and is easy to transport
It is defeated；Portugal's sweet dew glue is applied to the preservation field of sample by the present invention, provides a kind of new thinking for Saving specimen field, has
Higher application value.
Specific embodiment
It is further illustrated the present invention with reference to specific embodiment.Experimental method used in following embodiments is such as without spy
Different explanation, is conventional method, uses raw material, auxiliary agent etc., and unless otherwise specified, being can be from business such as conventional market purchases
The raw material and auxiliary agent that approach obtains.
Embodiment 1
Goldfish sample to be saved is cleaned up, concentration is that 5% formalin and alcohol solution dipping 12h consolidate
After fixed, Portugal's sweet dew glue gel that concentration is 2% is sealed up for safekeeping in vial, after preserving 3 years, the form of goldfish sample,
Color is unchanged, and limbs are soft, the phenomenon that limbs harden, deform do not occur.
Embodiment 2
Maple leaf sample to be saved is cleaned up, concentration is that 5% formalin and alcohol solution dipping 14h consolidate
After fixed, Portugal's sweet dew glue gel that concentration is 5% is sealed up for safekeeping in vial, after preserving 3 years, the form of maple leaf sample,
Color is unchanged, and limbs are soft, the phenomenon that limbs harden, deform do not occur.
Embodiment 3
Maple leaf sample to be saved is cleaned up, concentration is that 5% formalin and alcohol solution dipping 14h consolidate
After fixed, Portugal's sweet dew glue gel that concentration is 3% is sealed up for safekeeping in vial, after preserving 3 years, the form of maple leaf sample,
Color is unchanged, and limbs are soft, the phenomenon that limbs harden, deform do not occur.
Embodiment 4
Maple leaf sample to be saved is cleaned up, the formalin and alcohol solution dipping that concentration is 5% are solid for 24 hours
After fixed, Portugal's sweet dew glue gel that concentration is 6% is sealed up for safekeeping in vial, after preserving 3 years, the form of maple leaf sample,
Color is unchanged, and limbs are soft, the phenomenon that limbs harden, deform do not occur.
Embodiment 5
Butterfly specimen to be saved is cleaned up, after the alcohol solution dipping 16h that concentration is 5% is fixed, use is dense
It spends and is sealed up for safekeeping for 8% Portugal's sweet dew glue gel in vial, after preserving 3 years, the form of butterfly specimen, color are without change
Change, and limbs are soft, the phenomenon that limbs harden, deform do not occur.
Embodiment 6
Butterfly specimen to be saved is cleaned up, after the alcohol solution dipping 18h that concentration is 5% is fixed, use is dense
It spends and is sealed up for safekeeping for 8% Portugal's sweet dew glue gel and carragheen in vial, wherein, the matter of Portugal's sweet dew glue and carragheen
Amount is than being 2:1；After preserving 3 years, form, the color of butterfly specimen are unchanged, and limbs are soft, limbs hair do not occur
Firmly, the phenomenon that deforming, and gelatinous transparent is strong, can pass through the lines with vial and gel observation to butterfly.
Embodiment 7
Goldfish sample to be saved is cleaned up, after the alcohol solution dipping 18h that concentration is 5% is fixed, use is dense
It spends and is sealed up for safekeeping for 10% Portugal's sweet dew glue gel and carragheen in vial, wherein, the matter of Portugal's sweet dew glue and carragheen
Amount is than being 3:1；After preserving 3 years, form, the color of goldfish sample are unchanged, and limbs are soft, limbs hair do not occur
Firmly, the phenomenon that deforming, and gelatinous transparent is strong, can pass through the lines with vial and gel observation to goldfish.
Claims (4)
1. a kind of store method of sample, which is characterized in that the method is uses formalin and/or ethanol solution to handle institute
After stating sample 12~for 24 hours, the compound adhesive to be formed is blended using Portugal's sweet dew glue and carragheen and seals the sample up for safekeeping, the sample is water
The mass ratio of lively object, Portugal's sweet dew glue and carragheen is 2~3:1, the concentration of Portugal's sweet dew glue is 2~15%.
2. method according to claim 1, which is characterized in that the concentration of Portugal's sweet dew glue is 3~6%.
3. method according to claim 1, which is characterized in that the mass ratio of Portugal's sweet dew glue and carragheen is 2:1.
4. method according to claim 1, which is characterized in that handle the sample using formalin and/or ethanol solution
14~16h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610148749.XA CN105638641B (en) | 2016-03-16 | 2016-03-16 | Application and its application method of Portugal's sweet dew glue in Saving specimen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610148749.XA CN105638641B (en) | 2016-03-16 | 2016-03-16 | Application and its application method of Portugal's sweet dew glue in Saving specimen |
Publications (2)
| Publication Number | Publication Date |
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| CN105638641A CN105638641A (en) | 2016-06-08 |
| CN105638641B true CN105638641B (en) | 2018-05-29 |
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| CN201610148749.XA Expired - Fee Related CN105638641B (en) | 2016-03-16 | 2016-03-16 | Application and its application method of Portugal's sweet dew glue in Saving specimen |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108552164A (en) * | 2018-05-25 | 2018-09-21 | 宁德师范学院 | A kind of semisolid store method of plant specimen |
| CN110692626B (en) * | 2019-11-21 | 2022-02-18 | 钦州市中医药研究所 | Dry plant specimen and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003013239A2 (en) * | 2001-08-06 | 2003-02-20 | Peter Lamm | Method for the devitalisation of natural organs and/or for the preparation of extracellular matrices for tissue engineering |
| CN1666609A (en) * | 2004-03-10 | 2005-09-14 | 福建农林大学 | Fresh flower antistaling agent containing glucomannan |
| CN104837339A (en) * | 2012-10-18 | 2015-08-12 | 生命线科学有限公司 | Preservation of biomaterial properties and methods of storing |
| CN104911243A (en) * | 2015-06-29 | 2015-09-16 | 汇征联合（北京）医疗器械有限公司 | Solid culture medium for inoculating liquid samples and culture method |
-
2016
- 2016-03-16 CN CN201610148749.XA patent/CN105638641B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003013239A2 (en) * | 2001-08-06 | 2003-02-20 | Peter Lamm | Method for the devitalisation of natural organs and/or for the preparation of extracellular matrices for tissue engineering |
| CN1666609A (en) * | 2004-03-10 | 2005-09-14 | 福建农林大学 | Fresh flower antistaling agent containing glucomannan |
| CN104837339A (en) * | 2012-10-18 | 2015-08-12 | 生命线科学有限公司 | Preservation of biomaterial properties and methods of storing |
| CN104911243A (en) * | 2015-06-29 | 2015-09-16 | 汇征联合（北京）医疗器械有限公司 | Solid culture medium for inoculating liquid samples and culture method |
Non-Patent Citations (3)
| Title |
|---|
| 魔芋葡甘聚糖在固定化领域里的应用;朱琰 等;《生物技术》;20071231;第17卷(第6期);第91-94页 * |
| 魔芋葡甘聚糖物理共混研究新进展;李斌;《冷冻与速冻食品工业》;20040331;第10卷(第1期);第34-37、41页 * |
| 魔芋葡甘聚糖的研究进展及应用现状综述;刘楠 等;《安康学院学报》;20110831;第23卷(第4期);第95-98页 * |
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| CN105638641A (en) | 2016-06-08 |
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Effective date of registration: 20201030 Address after: 221700 Yuanzhao Village Industrial Zone, Zhao Zhuang Town, Fengxian County, Xuzhou, Jiangsu Patentee after: XUZHOU ZHONGREN LIGHTING Co.,Ltd. Address before: 510000, 602, 4, 106 strong street, Chancheng District, Guangdong, Foshan Patentee before: Ma heavy Wei |
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| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180529 |