CN100448351C - Specimen anti-corrosion stationary liquid - Google Patents
Specimen anti-corrosion stationary liquid Download PDFInfo
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- CN100448351C CN100448351C CNB2007100240486A CN200710024048A CN100448351C CN 100448351 C CN100448351 C CN 100448351C CN B2007100240486 A CNB2007100240486 A CN B2007100240486A CN 200710024048 A CN200710024048 A CN 200710024048A CN 100448351 C CN100448351 C CN 100448351C
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- chemical constitution
- stationary liquid
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- fatty alcohol
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Abstract
An example anti-corrosion fixing solution belongs to the field of preservative. The fixing solution comprises organic acid, fatty alcohol, amine, sulphone, organic amine salt and water; can be used for preserving human body example, animal example, and plant tissue example, and is free of aldehydes such as formaldehyde and acetaldehyde. The inventive example anti-corrosion fixing solution is characterized in innocuity with rat acute toxicity ig at a dose more than 50000mg/kg which is better than micro toxicity standard (5000mg/kg) of World Health Organization and safety 16.7 times and 1190 times higher than that of common salt and formaldehyde according to safety data reported in international authority literature, Preservation and fixing performance better than formalin. Example treated with the inventive fixing solution can be stored for a long time, and has clear histiocyte structure, high background contrast, and no repair for immunity blocking antigen, thereby simplifying immunity histochemistry experiment.
Description
Technical field
A kind of specimen anti-corrosion stationary liquid belongs to the preservative technology field.The present invention is a kind of human body, animal, anticorrosion, fixing solution of plant tissue specimens of being used for, and does not contain any aldehyde compounds such as formaldehyde, acetaldehyde.
Background technology
Formalin (formalin, the formaldehyde dilute solution) is the most frequently used human body, animal, plant tissue specimens preservative, formaldehyde in the formalin can combine with the amino of protein, protein generation irreversible denaturation, it is used for anticorrosion existing more than the 100 year history of human body, animal, plant tissue specimens.At hospital pathology department, Surgical Operating Room, formalin is used for the fixing of pathological tissue sample, and to prevent tissue automatic soup-dissolving, structural deterioration, cytomorphosis, tissue specimen is fixed with fixer after exsomatizing, and makes pathological section, carries out the medical pathologies diagnosis.Medical pathology diagnosis needs high-quality pathological section, and correct sample fixedly is the first step of making high quality slices.
Formalin has high toxicity, carcinogenicity, its toxicity is 30 times of methyl alcohol, long-term contact formalin may cause that chronic respiratory disease, cancer take place, as nasopharyngeal carcinoma, colon cancer, brain tumor, leukemia, cause memory and intelligence to descend, gastric-intestinal perforation, liver damage even stupor can occur when serious; The personnel of long-term contact formalin, the probability that the male sex gets cancer mortality is higher 6.8 times than noncontact personnel, and the women is high 9 times.
Summary of the invention
The purpose of this invention is to provide a kind of specimen anti-corrosion stationary liquid, can substitute formalin comprehensively, it is anticorrosion, fixing to be used for human body, animal, plant tissue specimens, and product is nontoxic, avoids the occupation infringement of formalin to place staff such as sample preservation, dissection, pathological diagnosis.
Technical scheme of the present invention: a kind of specimen anti-corrosion stationary liquid, form by organic acid, fatty alcohol, amine, sulfone, organic amine salt and water; The weight % content of each component is:
Organic acid 0.2-10
Fatty alcohol 45-85
Amine 0.5-5
Sulfone 0.3-6
Organic amine salt 0.3-3
Water 10-35
Described organic amine salt is: diaminoguanidine hydrochloride, 2,4,5,6-tetraminopyrimidine hydrochloride, pyridiniujm, aminoacetonitriles hydrochloride, 6-nitrobenzimidazole nitrate, 4-piperidone hydrochloride or monoethyl malonate sylvite.
Wherein the organic acid chemical constitution is:
Chemical constitution 1:CH
3(CH
2) nCOOH, n=0-8;
Chemical constitution 2:HOOC (CH
2) nCOOH, n=0-8;
Or chemical constitution 3:
n＝0-8，R
1，R
2：H，F，Cl，Br，OCH
3，CH
3。
Wherein the fatty alcohol chemical constitution is:
Chemical constitution 4:CH
3(CH
2) n OH, n=1-8;
Chemical constitution 5:HO (CH
2) nOH, n=2-8;
Or polyalcohol: glycerine, 1,2,4-butantriol, trimethylolpropane or pentaerythrite.
Wherein amine is: ethamine, diethylamine, triethylamine, tri-n-butylamine, two octodrines, monoethanolamine, triethanolamine or tri methylol amino methane.
Wherein sulfone is: sulfolane, dimethyl sulfone, divinylsulfone or dimethyl sulfoxide (DMSO).
Beneficial effect of the present invention:
(1) nontoxic.Chmice acute toxicity is irritated stomach dosage greater than 50,000mg/kg, the little malicious standard (5 of product that is better than the World Health Organization, 000mg/kg), according to internal authority bibliographical information salt, formaldehyde data of safety, specimen anti-corrosion stationary liquid safety of the present invention is higher 16.7 times and 1190 times respectively than salt, formaldehyde.
(2) anticorrosion, fixed performance is better than formalin.But with the sample long-term storage that specimen anti-corrosion stationary liquid of the present invention is handled, be used for pathological diagnosis fixedly the time, histocyte clear in structure, background contrasts height, SABC antigen are excused from a college course and are answered, and have simplified immunohistochemistry microsection manufacture program.
Embodiment
Embodiment 1
Specimen anti-corrosion stationary liquid consist of propionic acid 0.2%, ethanol 45%, tri methylol amino methane 0.5%, dimethyl sulfone 0.3%, 6-nitrobenzimidazole nitrate 0.3%, all the other are water.
Embodiment 2
Specimen anti-corrosion stationary liquid consist of acetate 5%, glycerine 85%, triethylamine 5%, sulfolane 0.3%, diaminoguanidine hydrochloride 0.3%, all the other are water.
Embodiment 3
Specimen anti-corrosion stationary liquid consist of benzoic acid 10%, propane diols 50%, two octodrines 3%, dimethyl sulfoxide (DMSO) 6%, pyridiniujm 3%, all the other are water.
Embodiment 4
Specimen anti-corrosion stationary liquid consist of ethanedioic acid 2%, 1,2,4-butantriol 61%, triethylamine 2.5%, divinylsulfone 3.5%, 2,4,5,6-tetraminopyrimidine hydrochloride 1.8%, all the other are water.
Embodiment 5
Specimen anti-corrosion stationary liquid consist of malonic acid 0.5%, pentaerythrite 47%, diethylamine 3%, dimethyl sulfone 2.8%, aminoacetonitriles hydrochloride 1%, all the other are water.
Embodiment 6
Specimen anti-corrosion stationary liquid consist of propionic acid 0.2%, ethanol 70%, tri methylol amino methane 0.5%, dimethyl sulfone 0.3%, 4-piperidone hydrochloride 1.5%, all the other are water.
Embodiment 7
Specimen anti-corrosion stationary liquid consist of phenylacetic acid 0.8%, propane diols 60%, monoethanolamine 2.5%, divinylsulfone 1.5%, monoethyl malonate sylvite 0.3%, all the other are water.
Embodiment 8
Specimen anti-corrosion stationary liquid consist of P-methoxybenzoic acid 1%, ethanol 50%, tri-n-butylamine 3%, dimethyl sulfoxide (DMSO) 1.6%, pyridiniujm 2%, all the other are water.
Claims (3)
1, a kind of specimen anti-corrosion stationary liquid is characterized by by organic acid, fatty alcohol, amine, sulfone, organic amine salt and water and forms; The weight % content of each component is:
Organic acid 0.2-10
Fatty alcohol 45-85
Amine 0.5-5
Sulfone 0.3-6
Organic amine salt 0.3-3
Water 10-35
Described organic amine salt is: diaminoguanidine hydrochloride, 2,4,5,6-tetraminopyrimidine hydrochloride, pyridiniujm, aminoacetonitriles hydrochloride, 6-nitrobenzimidazole nitrate or 4-piperidone hydrochloride;
Described organic acid chemical constitution is:
Chemical constitution 1:CH
3(CH
2) nCOOH, n=0-8;
Chemical constitution 2:HOOC (CH
2) nCOOH, n=0-8;
Or chemical constitution 3:
n＝0-8，R
1，R
2：H，F，Cl，Br，OCH
3，CH
3；
Described fatty alcohol chemical constitution is:
Chemical constitution 4:CH
3(CH
2) nOH, n=1-8;
Chemical constitution 5:HO (CH
2) nOH, n=2-8;
Or polyalcohol: glycerine, 1,2,4-butantriol, trimethylolpropane or pentaerythrite;
Described amine is selected for use: ethamine, diethylamine, triethylamine, tri-n-butylamine, two octodrines, monoethanolamine, triethanolamine or tri methylol amino methane;
Described sulfone is selected for use: sulfolane, dimethyl sulfone, divinylsulfone or dimethyl sulfoxide (DMSO).
2, specimen anti-corrosion stationary liquid according to claim 1 is characterized in that organic acid selects for use: acetate, propionic acid, ethanedioic acid, malonic acid, benzoic acid, phenylacetic acid or P-methoxybenzoic acid.
3, specimen anti-corrosion stationary liquid according to claim 1 is characterized in that fatty alcohol selects for use: ethanol, propane diols, glycerine, 1,2,4-butantriol or pentaerythrite.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2007100240486A CN100448351C (en) | 2007-07-12 | 2007-07-12 | Specimen anti-corrosion stationary liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2007100240486A CN100448351C (en) | 2007-07-12 | 2007-07-12 | Specimen anti-corrosion stationary liquid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101084746A CN101084746A (en) | 2007-12-12 |
| CN100448351C true CN100448351C (en) | 2009-01-07 |
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ID=38936047
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|---|---|---|---|
| CNB2007100240486A Expired - Fee Related CN100448351C (en) | 2007-07-12 | 2007-07-12 | Specimen anti-corrosion stationary liquid |
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Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101473812B (en) * | 2009-01-24 | 2011-09-14 | 温州医学院 | Antiseptic fixing liquid composition for cadaver sample and preparation method thereof |
| CN101984803B (en) * | 2010-09-26 | 2013-01-30 | 无锡市江原实业技贸总公司 | Specimen preserving, fixing and accelerating agent |
| CN102175496B (en) * | 2011-01-21 | 2013-04-03 | 哈尔滨格林标本技术开发有限公司 | Formaldehyde-free fixative for tissue sample |
| DE102013223384B4 (en) | 2013-11-15 | 2015-05-28 | Leica Biosystems Nussloch Gmbh | Fixation of tissue samples using aldehyde-releasing nitrogen-containing compounds |
| CN103988834A (en) * | 2014-05-18 | 2014-08-20 | 南阳医学高等专科学校 | Animal specimen preserving fluid |
| CN105432661B (en) * | 2015-07-08 | 2018-04-20 | 清华大学 | Discarded drosophila treatment fluid |
| CN105842033A (en) * | 2016-05-13 | 2016-08-10 | 北京九州柏林生物科技有限公司 | Formaldehyde-free tissue specimen stationary liquid |
| CN106332869B (en) * | 2016-11-01 | 2019-11-01 | 浙江译美生物科技有限公司 | Fat mesenchymal stem cell frozen stock solution and fat mesenchymal stem cell cryopreservation methods |
| CN106855475A (en) * | 2017-03-18 | 2017-06-16 | 王瑞才 | A kind of rapid tissue reagent treatment for pathological section |
| CN107560918A (en) * | 2017-09-22 | 2018-01-09 | 漯河医学高等专科学校 | A kind of pathological tissue specimen fixer |
| CN109377846B (en) * | 2018-10-11 | 2020-12-08 | 内蒙古科技大学包头医学院 | Method for making human anatomy viscera specimen |
| CN110132672B (en) * | 2019-05-16 | 2021-12-03 | 王樱达 | Tissue cell fixing method |
| CN112710526A (en) * | 2019-10-24 | 2021-04-27 | 广州江元医疗科技有限公司 | Cell fixing and preserving fluid and preparation method and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1086658A (en) * | 1993-08-19 | 1994-05-18 | 周正国 | Antiseptic preserving liquid for dead body specimen |
| WO1996028024A1 (en) * | 1995-03-09 | 1996-09-19 | Universidad Complutense De Madrid | Compositions containing dialkyl (c1-c6)-ketone peroxide for the preservation of organic tissues, and application of said compositions to the preservation and anatomical preparation of organic tissues of animal or human origin |
| WO1997022244A1 (en) * | 1995-12-15 | 1997-06-26 | Stiftelsen Facthor | Preservation solution |
| CN1233391A (en) * | 1998-04-30 | 1999-11-03 | 北京市燕化研新技术开发公司 | Preservation liquid for animal body specimen |
-
2007
- 2007-07-12 CN CNB2007100240486A patent/CN100448351C/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1086658A (en) * | 1993-08-19 | 1994-05-18 | 周正国 | Antiseptic preserving liquid for dead body specimen |
| WO1996028024A1 (en) * | 1995-03-09 | 1996-09-19 | Universidad Complutense De Madrid | Compositions containing dialkyl (c1-c6)-ketone peroxide for the preservation of organic tissues, and application of said compositions to the preservation and anatomical preparation of organic tissues of animal or human origin |
| WO1997022244A1 (en) * | 1995-12-15 | 1997-06-26 | Stiftelsen Facthor | Preservation solution |
| CN1233391A (en) * | 1998-04-30 | 1999-11-03 | 北京市燕化研新技术开发公司 | Preservation liquid for animal body specimen |
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| Publication number | Publication date |
|---|---|
| CN101084746A (en) | 2007-12-12 |
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Owner name: WUXI JIANGYUAN INDUSTRY COMPANY JIANGSU INSTITUTE Free format text: FORMER OWNER: JIANGSU PROV. INST. OF ATOMIC MEDICINE Effective date: 20110512 |
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Effective date of registration: 20110512 Address after: 214063 Jiangsu city of Wuxi province Qian Rong Lu No. 20 Patentee after: Wuxi Jiangyuan Industry Company Jiangsu Institute of Nuclear Medicine Address before: 214063 Jiangsu city of Wuxi province Qian Rong Lu No. 20 Patentee before: Jiangsu Prov. Inst. of Atomic Medicine |
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