CN106855475A - A kind of rapid tissue reagent treatment for pathological section - Google Patents
A kind of rapid tissue reagent treatment for pathological section Download PDFInfo
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- CN106855475A CN106855475A CN201710162504.7A CN201710162504A CN106855475A CN 106855475 A CN106855475 A CN 106855475A CN 201710162504 A CN201710162504 A CN 201710162504A CN 106855475 A CN106855475 A CN 106855475A
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- 230000001575 pathological effect Effects 0.000 title claims abstract description 31
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 29
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 40
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims abstract description 40
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 40
- 239000002994 raw material Substances 0.000 claims abstract description 34
- 239000007788 liquid Substances 0.000 claims abstract description 30
- 229960000583 acetic acid Drugs 0.000 claims abstract description 20
- 239000012362 glacial acetic acid Substances 0.000 claims abstract description 20
- 239000011780 sodium chloride Substances 0.000 claims abstract description 20
- 241000206575 Chondrus crispus Species 0.000 claims abstract description 19
- 239000012153 distilled water Substances 0.000 claims abstract description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 19
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims abstract description 16
- 239000012467 final product Substances 0.000 claims abstract description 15
- XZIIFPSPUDAGJM-UHFFFAOYSA-N 6-chloro-2-n,2-n-diethylpyrimidine-2,4-diamine Chemical compound CCN(CC)C1=NC(N)=CC(Cl)=N1 XZIIFPSPUDAGJM-UHFFFAOYSA-N 0.000 claims abstract description 14
- SNRUBQQJIBEYMU-UHFFFAOYSA-N Dodecane Natural products CCCCCCCCCCCC SNRUBQQJIBEYMU-UHFFFAOYSA-N 0.000 claims abstract description 14
- 125000003438 dodecyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 claims abstract description 14
- 229940035044 sorbitan monolaurate Drugs 0.000 claims abstract description 14
- 150000001412 amines Chemical class 0.000 claims abstract description 10
- 239000003146 anticoagulant agent Substances 0.000 claims abstract description 10
- 229940127219 anticoagulant drug Drugs 0.000 claims abstract description 10
- -1 Propane diols Chemical class 0.000 claims abstract description 8
- ATUOYWHBWRKTHZ-UHFFFAOYSA-N dimethylmethane Natural products CCC ATUOYWHBWRKTHZ-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000001294 propane Substances 0.000 claims abstract description 6
- ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 claims description 18
- 238000002360 preparation method Methods 0.000 claims description 14
- 238000003756 stirring Methods 0.000 claims description 14
- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 claims description 6
- 125000001664 diethylamino group Chemical group [H]C([H])([H])C([H])([H])N(*)C([H])([H])C([H])([H])[H] 0.000 claims description 2
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 1
- 229940124274 edetate disodium Drugs 0.000 claims 1
- 230000007170 pathology Effects 0.000 abstract description 4
- 230000006378 damage Effects 0.000 abstract description 3
- 238000003745 diagnosis Methods 0.000 abstract description 3
- 230000018044 dehydration Effects 0.000 abstract description 2
- 238000006297 dehydration reaction Methods 0.000 abstract description 2
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 12
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 12
- 238000000034 method Methods 0.000 description 8
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 7
- 239000003109 Disodium ethylene diamine tetraacetate Substances 0.000 description 6
- PIICEJLVQHRZGT-UHFFFAOYSA-N Ethylenediamine Chemical compound NCCN PIICEJLVQHRZGT-UHFFFAOYSA-N 0.000 description 6
- 235000019301 disodium ethylene diamine tetraacetate Nutrition 0.000 description 6
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 6
- HPNMFZURTQLUMO-UHFFFAOYSA-N diethylamine Chemical compound CCNCC HPNMFZURTQLUMO-UHFFFAOYSA-N 0.000 description 4
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical compound [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 description 3
- LLJZKKVYXXDWTB-UHFFFAOYSA-N acetic acid;sodium Chemical compound [Na].[Na].CC(O)=O LLJZKKVYXXDWTB-UHFFFAOYSA-N 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000012188 paraffin wax Substances 0.000 description 2
- 238000010827 pathological analysis Methods 0.000 description 2
- 208000014085 Chronic respiratory disease Diseases 0.000 description 1
- 206010010071 Coma Diseases 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 206010067125 Liver injury Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229960001484 edetic acid Drugs 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 231100000234 hepatic damage Toxicity 0.000 description 1
- 206010022694 intestinal perforation Diseases 0.000 description 1
- 230000008818 liver damage Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000029052 metamorphosis Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- ULWHHBHJGPPBCO-UHFFFAOYSA-N propane-1,1-diol Chemical class CCC(O)O ULWHHBHJGPPBCO-UHFFFAOYSA-N 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/305—Fixative compositions
- G01N2001/307—Fixative compositions non-toxic, no Hg, no formaldehyde
Abstract
The invention discloses a kind of rapid tissue reagent treatment for pathological section, include according to the raw material of weight portion：Propane diols or 5 30 parts of 60 150 parts of isopropanol, dodecyl ethyoxyl sulfobetaines or sorbitan monolaurate, 10 40 parts of acetone, 1 15 parts of carragheen, 1 10 parts of sodium chloride, 18 parts of anti-coagulants, 0.5 10 parts of amine, 1 15 parts of glacial acetic acid, 20 100 parts of distilled water.Weigh above-mentioned each raw material respectively according to weight portion, various liquid are put into beaker successively, during electromagnetic wand is put into liquid, 15 40min are stirred on electromagnetic oven and is obtained final product.The present invention can complete fixation, dehydration, the transparent one-stop treatment to pathological tissue, improve diagnosis speed, its 3 5h is just obtained a result, and significantly reduce patient and wait the pain seen a doctor, and be easy to clinician to take corresponding treatment in time.The present invention greatly reduces the body harm to pathology technician and the pollution to environment without formaldehyde, benzene class etc..
Description
Technical field
The present invention relates to Clinicopathologic Diagnosis reagent field, specifically a kind of rapid tissue for pathological section processes examination
Agent.
Background technology
Pathological cells tabletting technology is pathological diagnosis inalienable part, and the quality of tabletting technology directly affects disease
Can reason doctor make correct pathological diagnosis.Paraffin method is most widely used side in histology conven tional tabletting techniques
Method, paraffin section is applied not only to observe the morphosis of normal cell tissue, is also that the subjects such as pathology and medical jurisprudence are used to grind
Study carefully, observe and judge the main method of cell tissue metamorphosis.But had the following disadvantages in the method：The formal of use
Woods, dimethylbenzene etc. are volatile, have strong impulse and hypertoxicity high, to the working environment and living environment of pathology staff
All extremely harmful, Long Term Contact formalin may cause chronic respiratory disease, cancer to occur, and cause memory and intelligence
Decline, may occur in which that gastric-intestinal perforation, liver damage are even gone into a coma when serious；Organization processes all substeps are carried out, each mistake
Amount of reagent required for journey is big, and the tissue treatment time is long, and the harm to operating personnel is big.
The content of the invention
Greatly shortened it is an object of the invention to provide a kind of Diagnostic Time, safety non-pollution for the fast of pathological section
Fast tissue treatment reagent, to solve the problems, such as to be proposed in above-mentioned background technology.
To achieve the above object, the present invention provides following technical scheme：
A kind of rapid tissue reagent treatment for pathological section, includes according to the raw material of weight portion：Propane diols or isopropanol 60-
150 parts, dodecyl ethyoxyl sulfobetaines or sorbitan monolaurate 5-30 parts, acetone 10-40 parts, carragheen 1-15
Part, sodium chloride 1-10 parts, anti-coagulants 1-8 parts, amine 0.5-10 parts, glacial acetic acid 1-15 parts, distilled water 20-100 parts.
As further scheme of the invention：Amine is diethylamine, triethylamine or monoethanolamine.
As further scheme of the invention：Anti-coagulants is disodium ethylene diamine tetraacetate.
As further scheme of the invention：The rapid tissue reagent treatment for pathological section, according to weight portion
Raw material include：Propane diols or isopropanol 70-140 parts, dodecyl ethyoxyl sulfobetaines or sorbitan monolaurate
8-28 parts, acetone 12-35 parts, carragheen 1-12 parts, sodium chloride 1-8 parts, anti-coagulants 1-6 parts, amine 1-8 parts, glacial acetic acid 1-12 parts,
Distilled water 25-90 parts.
As further scheme of the invention：The rapid tissue reagent treatment for pathological section, according to weight portion
Raw material include：Propane diols or isopropanol 80-130 parts, dodecyl ethyoxyl sulfobetaines or sorbitan monolaurate
10-25 parts, acetone 15-30 parts, carragheen 2-10 parts, sodium chloride 2-8 parts, anti-coagulants 2-5 parts, amine 1-6 parts, glacial acetic acid 2-10
Part, distilled water 30-80 parts.
Another object of the present invention is to provide a kind of preparation method of the rapid tissue reagent treatment for pathological section, including
Following steps：Weigh above-mentioned each raw material respectively according to weight portion, various liquid are put into beaker successively, electromagnetic wand is put into liquid
In body, stirring 15-40min is obtained final product on electromagnetic oven.
Compared with prior art, the beneficial effects of the invention are as follows：
The present invention is by propane diols or isopropanol, dodecyl ethyoxyl sulfobetaines or sorbitan monolaurate, acetone, card
The rapid tissue reagent treatment of the compositions such as glue, sodium chloride, anti-coagulants, amine, glacial acetic acid is drawn, can complete to consolidate pathological tissue
Fixed, dehydration, transparent one-stop treatment, compare with traditional substep tissue treatment, improve diagnosis speed, its 3-5h is just obtained
Go out result, significantly reduce patient and wait the pain seen a doctor, be easy to clinician to take corresponding treatment in time.The present invention is free of
Formaldehyde, benzene class etc., greatly reduce the body harm to pathology technician and the pollution to environment；It is used for using the present invention
The section that pathological section is made, compared with the section that the tissue of traditional tissue treatment agent treatment is made, without obvious difference,
But Diagnostic Time is greatly shortened, the progress with conspicuousness.
Specific embodiment
Below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described,
Obviously, described embodiment is only a part of embodiment of the invention, rather than whole embodiments.Based in the present invention
Embodiment, the every other embodiment that those of ordinary skill in the art are obtained under the premise of creative work is not made, all
Belong to the scope of protection of the invention.
Embodiment 1
In the embodiment of the present invention, a kind of rapid tissue reagent treatment for pathological section includes according to the raw material of weight portion：Third
60 parts of glycol, 5 parts of dodecyl ethyoxyl sulfobetaines, 10 parts of acetone, 1 part of carragheen, 1 part of sodium chloride, ethylenediamine tetrem
1 part of acid disodium, 0.5 part of diethylamine, 1 part of glacial acetic acid, 20 parts of distilled water.
During preparation, above-mentioned each raw material is weighed respectively according to weight portion, various liquid are put into beaker successively, electromagnetic wand
Put into liquid, stirring 15min is obtained final product on electromagnetic oven.
Embodiment 2
In the embodiment of the present invention, a kind of rapid tissue reagent treatment for pathological section includes according to the raw material of weight portion：Third
150 parts of glycol, 30 parts of dodecyl ethyoxyl sulfobetaines, 40 parts of acetone, 15 parts of carragheen, 10 parts of sodium chloride, ethylenediamine
8 parts of tetraacethyl disodium, 10 parts of diethylamine, 15 parts of glacial acetic acid, 100 parts of distilled water.
During preparation, above-mentioned each raw material is weighed respectively according to weight portion, various liquid are put into beaker successively, electromagnetic wand
Put into liquid, stirring 40min is obtained final product on electromagnetic oven.
Embodiment 3
In the embodiment of the present invention, a kind of rapid tissue reagent treatment for pathological section includes according to the raw material of weight portion：Third
70 parts of glycol, 8 parts of dodecyl ethyoxyl sulfobetaines, 12 parts of acetone, 1 part of carragheen, 1 part of sodium chloride, ethylenediamine tetrem
1 part of acid disodium, 1 part of diethylamine, 1 part of glacial acetic acid, 25 parts of distilled water.
During preparation, above-mentioned each raw material is weighed respectively according to weight portion, various liquid are put into beaker successively, electromagnetic wand
Put into liquid, stirring 20min is obtained final product on electromagnetic oven.
Embodiment 4
In the embodiment of the present invention, a kind of rapid tissue reagent treatment for pathological section includes according to the raw material of weight portion：Third
140 parts of glycol, 28 parts of dodecyl ethyoxyl sulfobetaines, 35 parts of acetone, 12 parts of carragheen, 8 parts of sodium chloride, ethylenediamine tetraacetic
6 parts of acetic acid disodium, 8 parts of diethylamine, 12 parts of glacial acetic acid, 90 parts of distilled water.
During preparation, above-mentioned each raw material is weighed respectively according to weight portion, various liquid are put into beaker successively, electromagnetic wand
Put into liquid, stirring 35min is obtained final product on electromagnetic oven.
Embodiment 5
In the embodiment of the present invention, a kind of rapid tissue reagent treatment for pathological section includes according to the raw material of weight portion：Third
80 parts of glycol, 10 parts of sorbitan monolaurate, 15 parts of acetone, 2 parts of carragheen, 2 parts of sodium chloride, disodium ethylene diamine tetraacetate 2
Part, 1 part of triethylamine, 2 parts of glacial acetic acid, 30 parts of distilled water.
During preparation, above-mentioned each raw material is weighed respectively according to weight portion, various liquid are put into beaker successively, electromagnetic wand
Put into liquid, stirring 25min is obtained final product on electromagnetic oven.
Embodiment 6
In the embodiment of the present invention, a kind of rapid tissue reagent treatment for pathological section includes according to the raw material of weight portion：It is different
130 parts of propyl alcohol, 25 parts of dodecyl ethyoxyl sulfobetaines, 30 parts of acetone, 10 parts of carragheen, 8 parts of sodium chloride, ethylenediamine tetraacetic
5 parts of acetic acid disodium, 6 parts of triethylamine, 10 parts of glacial acetic acid, 80 parts of distilled water.
During preparation, above-mentioned each raw material is weighed respectively according to weight portion, various liquid are put into beaker successively, electromagnetic wand
Put into liquid, stirring 30min is obtained final product on electromagnetic oven.
Embodiment 7
In the embodiment of the present invention, a kind of rapid tissue reagent treatment for pathological section includes according to the raw material of weight portion：It is different
60 parts of propyl alcohol, 5 parts of sorbitan monolaurate, 10 parts of acetone, 1 part of carragheen, 1 part of sodium chloride, disodium ethylene diamine tetraacetate 1
Part, 0.5 part of triethylamine, 1 part of glacial acetic acid, 20 parts of distilled water.
During preparation, above-mentioned each raw material is weighed respectively according to weight portion, various liquid are put into beaker successively, electromagnetic wand
Put into liquid, stirring 40min is obtained final product on electromagnetic oven.
Embodiment 8
In the embodiment of the present invention, a kind of rapid tissue reagent treatment for pathological section includes according to the raw material of weight portion：It is different
150 parts of propyl alcohol, 30 parts of sorbitan monolaurate, 40 parts of acetone, 15 parts of carragheen, 10 parts of sodium chloride, ethylenediamine tetra-acetic acid two
8 parts of sodium, 10 parts of triethylamine, 15 parts of glacial acetic acid, 100 parts of distilled water.
During preparation, above-mentioned each raw material is weighed respectively according to weight portion, various liquid are put into beaker successively, electromagnetic wand
Put into liquid, stirring 15min is obtained final product on electromagnetic oven.
Embodiment 9
In the embodiment of the present invention, a kind of rapid tissue reagent treatment for pathological section includes according to the raw material of weight portion：It is different
140 parts of propyl alcohol, 8 parts of sorbitan monolaurate, 35 parts of acetone, 1 part of carragheen, 8 parts of sodium chloride, disodium ethylene diamine tetraacetate 1
Part, 8 parts of monoethanolamine, 1 part of glacial acetic acid, 90 parts of distilled water.
During preparation, above-mentioned each raw material is weighed respectively according to weight portion, various liquid are put into beaker successively, electromagnetic wand
Put into liquid, stirring 20min is obtained final product on electromagnetic oven.
Embodiment 10
In the embodiment of the present invention, a kind of rapid tissue reagent treatment for pathological section includes according to the raw material of weight portion：It is different
70 parts of propyl alcohol, 28 parts of sorbitan monolaurate, 12 parts of acetone, 12 parts of carragheen, 1 part of sodium chloride, disodium ethylene diamine tetraacetate 6
Part, 1 part of monoethanolamine, 12 parts of glacial acetic acid, 25 parts of distilled water.
During preparation, above-mentioned each raw material is weighed respectively according to weight portion, various liquid are put into beaker successively, electromagnetic wand
Put into liquid, stirring 30min is obtained final product on electromagnetic oven.
Embodiment 11
In the embodiment of the present invention, a kind of rapid tissue reagent treatment for pathological section includes according to the raw material of weight portion：Third
80 parts of glycol, 25 parts of sorbitan monolaurate, 15 parts of acetone, 10 parts of carragheen, 2 parts of sodium chloride, disodium ethylene diamine tetraacetate 5
Part, 1 part of monoethanolamine, 10 parts of glacial acetic acid, 30 parts of distilled water.
During preparation, above-mentioned each raw material is weighed respectively according to weight portion, various liquid are put into beaker successively, electromagnetic wand
Put into liquid, stirring 35min is obtained final product on electromagnetic oven.
Embodiment 12
In the embodiment of the present invention, a kind of rapid tissue reagent treatment for pathological section includes according to the raw material of weight portion：It is different
130 parts of propyl alcohol, 10 parts of dodecyl ethyoxyl sulfobetaines, 30 parts of acetone, 2 parts of carragheen, 8 parts of sodium chloride, ethylenediamine tetraacetic
2 parts of acetic acid disodium, 6 parts of monoethanolamine, 2 parts of glacial acetic acid, 80 parts of distilled water.
During preparation, above-mentioned each raw material is weighed respectively according to weight portion, various liquid are put into beaker successively, electromagnetic wand
Put into liquid, stirring 40min is obtained final product on electromagnetic oven.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be in other specific forms realized.Therefore, no matter
From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit requires to be limited rather than described above, it is intended that all in the implication and scope of the equivalency of claim by falling
Change is included in the present invention.
Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each implementation method is only wrapped
Containing an independent technical scheme, this narrating mode of specification is only that for clarity, those skilled in the art should
Specification an as entirety, the technical scheme in each embodiment can also be formed into those skilled in the art through appropriately combined
May be appreciated other embodiment.
Claims (6)
1. a kind of rapid tissue reagent treatment for pathological section, it is characterised in that the raw material according to weight portion includes：The third two
Alcohol or isopropanol 60-150 parts, dodecyl ethyoxyl sulfobetaines or sorbitan monolaurate 5-30 parts, acetone 10-40
Part, carragheen 1-15 parts, sodium chloride 1-10 parts, anti-coagulants 1-8 parts, amine 0.5-10 parts, glacial acetic acid 1-15 parts, distilled water 20-100
Part.
2. the rapid tissue reagent treatment for pathological section according to claim 1, it is characterised in that amine is diethyl
Amine, triethylamine or monoethanolamine.
3. the rapid tissue reagent treatment for pathological section according to claim 1, it is characterised in that anti-coagulants is second
Edetate disodium.
4. the rapid tissue reagent treatment for pathological section according to claim 1, it is characterised in that according to weight portion
Raw material include：Propane diols or isopropanol 70-140 parts, dodecyl ethyoxyl sulfobetaines or sorbitan monolaurate
8-28 parts, acetone 12-35 parts, carragheen 1-12 parts, sodium chloride 1-8 parts, anti-coagulants 1-6 parts, amine 1-8 parts, glacial acetic acid 1-12 parts,
Distilled water 25-90 parts.
5. the rapid tissue reagent treatment for pathological section according to claim 4, it is characterised in that according to weight portion
Raw material include：Propane diols or isopropanol 80-130 parts, dodecyl ethyoxyl sulfobetaines or sorbitan monolaurate
10-25 parts, acetone 15-30 parts, carragheen 2-10 parts, sodium chloride 2-8 parts, anti-coagulants 2-5 parts, amine 1-6 parts, glacial acetic acid 2-10
Part, distilled water 30-80 parts.
6. the preparation method of a kind of rapid tissue reagent treatment for pathological section as described in claim 1-5 is any, its
It is characterised by, comprises the following steps：Weigh above-mentioned each raw material respectively according to weight portion, various liquid are put into beaker successively,
During electromagnetic wand is put into liquid, on electromagnetic oven stirring 15-40min obtains final product.
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| CN201710162504.7A CN106855475A (en) | 2017-03-18 | 2017-03-18 | A kind of rapid tissue reagent treatment for pathological section |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108414308A (en) * | 2018-01-31 | 2018-08-17 | 四川农业大学 | A kind of Fish histopathology slice decalcifying Fluid |
| GB2576331A (en) * | 2018-08-14 | 2020-02-19 | Apacor Ltd | Fixative solution and method of preparation of biological sample for examination |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101084746A (en) * | 2007-07-12 | 2007-12-12 | 江苏省原子医学研究所 | Specimen anti-corrosion stationary liquid |
| US20090136992A1 (en) * | 2003-10-24 | 2009-05-28 | The University Of Miami | Simplified tissue processing |
| CN105092328A (en) * | 2015-07-30 | 2015-11-25 | 厦门宝太生物科技有限公司 | Erythrocyte removing treating fluid and application |
| CN105300753A (en) * | 2015-09-10 | 2016-02-03 | 山东骏腾医疗科技有限公司 | Fast tissue treatment reagent for pathological section |
| CN106018050A (en) * | 2016-05-20 | 2016-10-12 | 北京九州柏林生物科技有限公司 | Frozen section embedding agent |
-
2017
- 2017-03-18 CN CN201710162504.7A patent/CN106855475A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090136992A1 (en) * | 2003-10-24 | 2009-05-28 | The University Of Miami | Simplified tissue processing |
| CN101084746A (en) * | 2007-07-12 | 2007-12-12 | 江苏省原子医学研究所 | Specimen anti-corrosion stationary liquid |
| CN105092328A (en) * | 2015-07-30 | 2015-11-25 | 厦门宝太生物科技有限公司 | Erythrocyte removing treating fluid and application |
| CN105300753A (en) * | 2015-09-10 | 2016-02-03 | 山东骏腾医疗科技有限公司 | Fast tissue treatment reagent for pathological section |
| CN106018050A (en) * | 2016-05-20 | 2016-10-12 | 北京九州柏林生物科技有限公司 | Frozen section embedding agent |
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| CN108414308A (en) * | 2018-01-31 | 2018-08-17 | 四川农业大学 | A kind of Fish histopathology slice decalcifying Fluid |
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