CN113876650B

CN113876650B - Cosmetic composition containing okra sprouting exudates

Info

Publication number: CN113876650B
Application number: CN202111126471.3A
Authority: CN
Inventors: 黄淳锡; 金恩智; 张曼; 崔亨振; 金荣熙; 金延埈
Original assignee: Ruidian Co; Shanghai Ruidian Biotechnology Co ltd; Cosmax China Cosmetics Co Ltd
Current assignee: Ruidian Co; Shanghai Ruidian Biotechnology Co ltd; Cosmax China Cosmetics Co Ltd
Priority date: 2021-07-06
Filing date: 2021-09-26
Publication date: 2023-10-20
Anticipated expiration: 2041-09-26
Also published as: KR20230007836A; KR102555539B1; CN113876650A

Abstract

The invention provides a cosmetic composition comprising okra germinated exudates. Aiming at taking the cells of the dermis layer and the epidermis layer as objects, the synthesis of the collagen of the dermis layer and the ceramide of the epidermis layer is induced while the moisture content is improved, and a raw material capable of reducing the structural moisture loss is developed. Meanwhile, in the epidermis layer of the skin, the hydration degree of the stratum corneum is improved by taking the stratum corneum as a target, and a raw material capable of reducing the water loss of the outermost layer is developed. In addition, the invention provides a cosmetic raw material composition which does not contain harmful substances, resists wrinkles and aging and has excellent moisturizing effect through an environment-friendly and low-cost process.

Description

Cosmetic composition containing okra sprouting exudates

Technical Field

The invention relates to a cosmetic raw material composition, which comprises okra exudates as an active ingredient and is used for moisturizing skin.

Background

The skin has the ability to protect our body from adverse factors such as physical, mechanical, chemical, pathogenic microorganisms originating from the external environment.

The cosmetics are used for cleaning skin or making the skin more beautiful and protecting the skin from damaging the external environment, i.e. achieving the purposes of cleaning, eliminating bad smell, protecting skin, beautifying and finishing.

With the increasing attention to the environment worldwide, the cosmetic market also generally adopts environmental protection technology in the aspects of raw materials, packaging materials and the like, and for plant raw materials used for cosmetics, people only pay attention to the environment for obtaining active ingredients from the nature, and the environment protection problem related to the process and the like is not included.

The natural raw material extract (extract) used in the current cosmetics mainly adopts methods of high-temperature cooking, organic solvents, ultrasonic waves and the like to extract active ingredients in plants.

The high-temperature cooking extraction method cannot efficiently extract the effective components due to heat damage, and decomposition or denaturation of the components may occur; the extraction method using an organic solvent has a disadvantage of consuming a large amount of energy because an additional process such as completely removing residual solvents harmful to human bodies is required in order to use the extract as a cosmetic raw material.

In addition, when the collected plant material is used, there is a risk that harmful substances such as heavy metals and pesticide residues contained in soil and water used for cultivating plants remain in the plant material.

On the other hand, in recent years, the cosmetic industry has been very concerned with water injection, and as consumers who view the full ingredients of cosmetics are increasingly growing, purified water as the main ingredient of cosmetics is also being treated differently. While ingredients that are gentle to the skin with little irritation are used, various natural waters such as glacier water, hot spring water, deep ocean water, white birch water, etc. are favored, and such waters contain various natural-derived ingredients such as minerals.

Collagen contained in cosmetics is widely used as an effective ingredient for inhibiting wrinkles and reducing the formation of wrinkles because of maintaining the skin structure and function. These collagens have a function of retaining the moisture factor in the dermis layer, and therefore, the denser the collagen is, the more moisture loss is prevented, and the moisture of the skin is maintained. In order to keep the moisture of the skin, the moisture content of the skin should be increased, and the moisture is not lost.

The other component is mucin, which is a viscous substance found in animals and plants, and can increase collagen and elastin in skin and improve skin elasticity. The chondroitin contained therein can prevent moisture loss of the skin and helps to maintain the moist state of the skin. In the past years, "snail cream" has been widely known for its anti-aging effect, and recently, plant mucin (phyto-mucin) which is not animal has also come to be attracting attention (patent document 1).

Viscous proteins are generally found in plants such as yam, lotus root, and taro, and are also found in a large amount in okra, a subtropical vegetable (patent document 2). In particular, okra contains not only viscous protein, but also abundant inorganic matters, vitamins, proteins, dietary fibers and the like.

In order to extract viscous proteins as raw materials of cosmetics from a general plant, it is necessary to use an organic solvent for precipitation through a plurality of steps such as immersing, stirring, separating, and drying (patent document 3). The viscous protein thus obtained is used as a raw material for cosmetics, and a plurality of steps are required from the extraction of the viscous protein to the production of the raw material for cosmetics.

Citation of technical literature

Patent literature

(patent document 0001) korean patent registration No. 10-1490462, (patent document 0002) korean patent registration No. 10-1397160, japanese patent publication No. 2008-127397.

Disclosure of Invention

The invention aims to develop a raw material for reducing the water loss on the skin structure by taking dermis and epidermis cells as objects, and inducing the synthesis of collagen of the dermis and ceramide of the epidermis while improving the water content.

Another object of the present invention is to provide a cosmetic raw material composition free of harmful substances, resistant to wrinkles, resistant to aging, and having excellent moisturizing effect through an environment-friendly and low-cost process.

Preferably, the cosmetic raw material composition of the present invention comprises okra germinated exudates.

Preferably, the exudate may promote one or more of collagen synthesis and hyaluronic acid synthesis.

Preferably, the exudate may promote expression of one or more of ceramide synthase and hyaluronic acid synthase.

Preferably, the exudates are obtained by circularly cultivating okra seeds, but are not limited thereto.

More preferably, in the circulating cultivation, distilled water 40 to 60 times the total weight of the seeds is used for germination.

Preferably, the exudates include one or more of polyphenols, mucins, kinetin, gibberellins, prolines.

The cosmetic raw material composition may include one or more additives selected from butylene glycol, hexylene glycol, ethylhexyl glycerol, and phenoxyethanol.

The cosmetic raw material composition can be any one of a lotion, a nourishing cream, a massage cream, an essence, a facial mask, a gel, an emulsion, an ointment, a patch and a spray selected from a skin softening lotion, a nourishing lotion and the like.

The cosmetic raw material composition is prepared from the natural plant okra serving as a raw material through an environment-friendly and low-cost process, does not contain harmful substances, has the effects of resisting wrinkles and skin aging, and accords with the ancient house protocol aiming at protecting biological resources.

Drawings

FIG. 1 is a schematic diagram of the results of test example 2 of the present invention.

FIG. 2 is a schematic diagram of the results of test example 3 according to the present invention.

Fig. 3 and 4 are schematic diagrams showing the results of test example 4 of the present invention.

Detailed Description

The present invention will be described in further detail with reference to specific examples so as to enable those skilled in the art to which the invention pertains to practice the invention. The embodiments of the present invention are provided to more fully explain the present invention to those having ordinary skill in the art. Therefore, the embodiment of the present invention may be modified in various ways, and the scope of the present invention is not limited to the following embodiment.

In the description of the present invention, the certain portion "includes" certain constituent elements means that other constituent elements are not excluded when no special exclusive meaning is stated, but other constituent elements are also included.

In the present description, when the certain step is "above" or "before" the certain step, it includes not only the case that the certain step has a direct timing relationship with other certain steps, but also the case that the steps are mixed with each other later, the sequential timing sequences of the two steps are interchangeable, and the case that there is an indirect timing relationship, which is also protected.

The terms "about" and "actually" and the like used in the description of the present invention are used in the meaning of numerical value or value close to the numerical value thereof when they provide an allowable error of inherent manufacturing and substance in the sense of the present invention, and are used for the purpose of helping understanding the present invention and also for the purpose of preventing a malicious infringer from illegally utilizing contents that are accurate or disclosed with accurate numerical values. In this specification, the term "… step" or "… step" is used not to represent "step for …".

During the growth of plants, various chemical substances are secreted in order to inhibit the growth of other plants and promote their growth. In the absence of physical effects on plants, substances naturally secreted from plants are called exudates (exudates). Exudates from plants are mainly secreted from the roots, but may also be secreted from other parts.

With the official validation of the famous and ancient house agreement as a plant resource protection policy, biological resource protection policies are actively implemented in all countries of the world. The invention can effectively utilize about 10-20% of exudates of all photosynthetic products secreted from plant roots, and simultaneously achieve the purposes of protecting plant resources and efficiently obtaining active ingredients.

Plant exudates are generally used as effective components by recycling sprout exudates growing in the germination process of soilless culture seeds, so that the extraction of the effective components is realized in a green and environment-friendly way.

The germination process is to ensure that exudates are expelled during plant growth, which is an effective process to improve the nutritional quality and functionality of the plant. Thus, exudates secreted from the sprouts may obtain higher nutritional components than seeds. This is due to the fact that the sprouts have 3-4 times more physiologically active substance than the adult active substance content.

According to the invention, by obtaining the exudates from the plants sprouted in soilless culture, the components can be ensured not to be decomposed and denatured, and the active ingredients can be concentrated while sprouting can be realized by circular cultivation, so that the invention has the advantages of energy saving and environmental protection.

In addition, since the plant exudates are carried out from the seed germination step, there is no risk factor of harmful components such as heavy metals remaining in the raw materials.

The root exudates of the present invention have the advantage of being used as natural water, as in various natural water materials which have been recently favored in the cosmetic field.

The cosmetic raw material composition of the present invention further includes the use of natural water used in the cosmetic field.

In addition, when plant exudates are used according to the present invention, components which have not been used in conventional extracts can be used.

Specifically, various exudates are secreted from plant roots, wherein sugars, amino acids, organic acids, etc. account for a large specific gravity.

It is known that saccharides have skin moisturizing effect, and particularly polyphenols have both anticancer effect and heart disease preventing effect as superior antioxidant substances.

In addition, amino acids have the function of producing collagen in the skin and natural moisturizing factors in the stratum corneum.

In addition, a large amount of plant hormone exists in plant root exudates, and the plant hormone has the functions of regulating signals such as plant growth, differentiation, defense, aging regulation and the like. Phytohormones such as kinetin and gibberellin contained in plant root exudates have been identified as having the effect of promoting biosynthesis of skin collagen, thereby imparting moisture retention to the skin.

These phytohormones are present in very low concentrations in plants and are difficult to isolate and quantify, and therefore are more readily available from plant root exudates.

Plant extracts from different plant species, the plant root exudates are sometimes higher in phytohormone content than the plant extracts from the sprouts, such as an amino acid associated with skin moisturization, proline in the exudates from the plant root. Therefore, the root exudates of the present invention are more excellent in performance and lower in cost than conventional plant extracts.

In the exudates, the ensured exudates contain viscous proteins, and the exudates are directly used as raw materials of cosmetics, so that the process is simple and the production efficiency is high.

Specifically, the okra extract obtained from 70% ethanol was 22.69ppm in viscous protein and 15.32ppm in exudates, based on 0.01% of the dry residue, compared with okra sprout exudates. Namely, the content of the okra sprout exudate is not much different from that of the ethanol extract using the organic solvent, and the extract is extracted without a supplementary process in the exudate, so that the efficiency is higher when the okra sprout exudate is used for developing the cosmetic raw material containing the viscous protein.

To manufacture and evaluate the efficacy of okra germination exudates of the present invention, the following tests were conducted.

Example 1: preparation of okra sprouting exudates

(1) Okra seeds occupy 50% -80% of the area of the germination equipment. The seeds do not always sprout 100%, and when the seed area is 50% or less, the content of the active ingredient in the exudate becomes small, and when the seed area is 80% or more, the area after germination becomes small, which affects the growth of the sprouts.

(2) The germination apparatus was connected to an automatic circulation filter, and then germination was performed with distilled water 40-60 times the total weight of the seeds for 8 days, and the growth of microorganisms was prevented. When the distilled water content is less than 40 times, the amount of the exudates is small, and the exudates preparation efficiency is low; when the distilled water content is more than 60 times, the concentration of the active ingredient in the exudates is lowered, and too high and too low are not preferable.

The germination equipment was started and the automatic circulation device was started, and the germination equipment was filtered through a 0.2 μm pes filter every 10 minutes to inhibit the proliferation of microorganisms in the exudate, and the finally obtained exudate was stored at 4 ℃.

Test example 1: protein and polysaccharide content detection

The protein content is detected by BCA assay, and the polysaccharide content is detected by phenol-sulfuric acid method. Along with the increase of the germination time of okra seeds, the protein and polysaccharide contents of okra seeds are gradually increased. The results are shown in Table 1 below.

TABLE 1

When the germination time is less than 8 days, the growth of the sprouts is insufficient, and the contents of the protein, polysaccharide and other components are also insufficient. In contrast, when the germination time exceeds 8 days, the polysaccharide content is reduced due to the reabsorption inside plants and the use as a nutrient component of microorganisms, which is disadvantageous for filtration and recovery.

Test example 2: collagen biosynthesis assay

Collagen is the main structural protein of skin and accounts for 70-80% of dermis. Collagen gradually decreases or changes in structure in the skin due to endogenous aging and photoaging. Since collagen has a function of retaining moisture in the dermis layer, the more compact the collagen is, the more moisture loss of the skin can be prevented. Collagen cannot be absorbed transdermally by being smeared on the skin surface due to the large molecular weight, so that the biosynthesis of collagen in the skin is more effective in realizing skin moisture retention.

The expression of the type I procollagen C-peptide content in human dermal fibroblast (HDFn) biological fluid was detected using Takara's Procollagen Type I C-peptide (PIP) EIA Kit.

As a result, as shown in FIG. 1, the expression level of PIP-1 showed an increasing trend in all cases after the treatment of the exudate from day 8 of okra germination.

Test example 3: synthesis detection of Hyaluronic acid (Hyaluronic acid)

Hyaluronic acid present in the skin can be produced in human dermal fibroblasts and keratinocytes, which are constituents of mucopolysaccharides. Hyaluronic acid is known to be the most representative moisturizing factor for skin because it absorbs a large amount of moisture, and it can be combined with 1000 times of moisture per se to effectively retain skin moisture.

The results of the examination by Echellon company Hyaluronan Enzyme-Linked mmunosorbent Assay Kit show that the change in hyaluronic acid expression can be found in human epidermal keratinocytes (HEKn) and human dermal fibroblasts (HDFn).

As shown in fig. 2, in human epidermal keratinocytes (HEKn), expression of hyaluronic acid increased at all concentrations when treated with day 8 exudate from okra germination.

Test example 4: ceramide synthase 3 (CERS 3), detection of Hyaluronan synthase (HAS 2) expression

Ceramide, which is composed of lipid components, accounts for 35-40% of intercellular colloid (intercellular cement) in epidermis layer, forms skin barrier, and prevents water loss. If ceramide decreases, the lamellar (lamella) structure is destroyed, the percutaneous water loss increases, and the skin becomes dry.

CERS3 is a ceramide synthase that induces ceramide synthesis and acts to inhibit loss of moisture from the epidermis layer of the skin.

HAS2 is a synthetic enzyme of the representative moisturizing factor hyaluronic acid, which promotes the synthesis of hyaluronic acid, helping to preserve moisture in the skin.

The expression changes of CERS3 and HAS2 in human epidermal keratinocytes (HEKn) and human dermal fibroblasts (HDFn) were examined by RT-PCR.

As a result, as shown in FIG. 3, when the okra was used to germinate the day 8 exudate, it was found that the expression of CERS3 was increased at all concentrations, and the expression of HAS2 was increased at a concentration of 0.5% or more.

Test example 5: detection of total polyphenol content

Polyphenols are useful for skin health, and are therefore often used as effective ingredients in various cosmetics. To determine the polyphenol content of okra germinated exudates, the Folin-Ciocalteu colorimetric assay was used and the results are shown in Table 2.

As shown in Table 2, the content of polyphenols contained in the sprouted exudates increased with the number of days of sprouting of okra. When the germination time is less than 8 days, the growth of sprouts is insufficient and the content of components such as polyphenol is not high enough.

TABLE 2

#	Name of the name	Total polyphenol (μg/ml)
			1	Exudate from the next day of germination	5.08
2	Germinating the fourth day exudates	5.54
			3	Germinating the sixth day exudates	7.51
4	Exudate from eighth day of germination	9.63

The cosmetic raw material composition may further comprise at least one additive selected from butanediol, hexanediol, ethylhexyl glycerol and phenoxyethanol. The additives are required to form the subsequent dosage form.

The cosmetic raw material composition may be any one of a lotion, a nourishing cream, a massage cream, an essence, a mask, a gel, an emulsion, an ointment, a patch, and a spray selected from a lotion, a nourishing lotion, and the like, but is not limited thereto.

While the embodiments of the present invention have been described in detail, the scope of the present invention is not limited thereto, and those skilled in the art will appreciate that various modifications and variations are possible without departing from the technical spirit of the claims.

Claims

1. A cosmetic raw material composition comprising okra germinated exudates prepared by: the okra seeds occupy 50% -80% of the area of the germination equipment, distilled water which is 40-60 times of the total weight of the seeds is added, the germination equipment is started, and germination is carried out for 8 days; the germination equipment is connected with the automatic circulation device, and the automatic circulation device is started, and the germination equipment is filtered through a 0.2 ㎛ PES filter every 10 minutes to inhibit the proliferation of microorganisms in the exudates, so that the okra germination exudates are finally obtained.

2. The cosmetic raw material composition according to claim 1, characterized in that: the cosmetic raw material composition further comprises more than one additive selected from butanediol, hexanediol, ethylhexyl glycerol and phenoxyethanol.

3. The cosmetic raw material composition according to claim 1, characterized in that: the cosmetic raw material composition is skin softening lotion, nourishing cream, massage cream, essence, facial mask, gel, lotion, ointment, patch or spray.

4. Use of okra germination exudates according to claim 1, in the preparation of a composition for promoting collagen synthesis.

5. Use of okra germination exudates according to claim 1, for the preparation of a composition promoting ceramide synthesis.