CN108938503A - A kind of Abelmoschus Esculentus Linn extract and its preparing the application in cosmetics - Google Patents
A kind of Abelmoschus Esculentus Linn extract and its preparing the application in cosmetics Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61Q19/08—Anti-ageing preparations
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
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Abstract
The invention discloses a kind of Abelmoschus Esculentus Linn extract and its preparing the application in cosmetics, the following steps are included: the preparation method of the okra colloid polysaccharide: S1: weighing 8~15g okra dry powder, food acid buffer is chosen as extracting solution, weigh cellulase of the 6~12g through overactivation, then okra dry powder, extracting solution and cellulase are successively added in reactor tank, obtain the leaching liquor of okra colloid polysaccharide；S2: and then the leaching liquor in S1 is poured into centrifuge centrifugation and extracts clear liquid, clear liquid is moved on Rotary Evaporators and does concentration；S3: backward S2 in concentrate in addition 2.5 times of volume dehydrated alcohols be centrifuged again；S4: the concentrate after being centrifuged in S3 is removed into clear liquid, concentration is prepared into okra colloid polysaccharide, it is applied in skin protection cosmetics by Abelmoschus Esculentus Linn extract, the cosmetics being produced have good emulsibility, water imbibition and high viscosity, play the role of improving the colour of skin, anti-aging and effective moisturizing.
Description
Technical field
The present invention relates to cosmetics processing technique field more particularly to a kind of Abelmoschus Esculentus Linn extract and its preparing cosmetics
In application.
Background technique
Okra is also known as Abelmoschus esculentus, is Malvaceae annual herb plant.Stem is cylindrical, and sparsely grow dissipates thorn.Leaf palmate, splits
Piece is wealthy to narrow, and stipule is linear, by thin bristle.Hua Dan is born between axil, and bennet is dredged by rough bristle, and squamella is bell；Calyx is bell,
It is close by starlike down；Flower yellow, inner face base portion purple, capsule tubular steeple shape, seed is spherical, has nerville line.Okra master
It is divided into two classes, it is respectively red to cross okra and green fruit okra, it is mostly green or dark violet red.Since people are to Novel ring
The attention rate and attention degree for protecting vegetables greatly improve, and market demand is growing, therefore also promote okra cultivation skill
The development of art and deep process technology.
Tender okra pod extract has good medicinal and health-care effect, can also apply in cosmetic industry, and existing
In some Cosmetic Manufacture market, generally all chemical synthesis substance is used as a raw material for production, and chemically synthesized cosmetics are former
Harmful components office in material is more, and is easy to cause irritating injury to skin, and the effective component in natural plants can be with
Skin permeation fundamentally improves the delicate degree of skin, enhances the tight of skin by improving the structure and material base of skin
Cause property and smoothness.
Therefore, it is used to solve above-mentioned ask we have proposed a kind of Abelmoschus Esculentus Linn extract and its preparing the application in cosmetics
Topic.
Summary of the invention
The purpose of the present invention is to solve existing in the prior art in existing Cosmetic Manufacture market, generally all use
As a raw material for production, and the harmful components office in chemically synthesized cosmetic material is more for chemical synthesis substance, and is easy to skin
Skin causes the shortcomings that irritating injury, and a kind of Abelmoschus Esculentus Linn extract that proposes and its is preparing the application in cosmetics.
To achieve the goals above, present invention employs following technical solutions:
A kind of Abelmoschus Esculentus Linn extract, including okra colloid polysaccharide, protein, fat and flavone compound；
The preparation method of the okra colloid polysaccharide the following steps are included:
S1: weighing 8~15g okra dry powder, and then choosing pH value is 6 food acid buffer as extracting solution, then
Cellulase of the 6~12g through overactivation is weighed, okra dry powder, extracting solution and cellulase are successively then added to reaction
In tank, and the time of enzymatic hydrolysis, the temperature of enzymatic hydrolysis, solid-liquid ratio progress constant temperature enzymatic hydrolysis are set, obtain the extraction of okra colloid polysaccharide
Liquid；
S2: and then pouring into the leaching liquor in S1 in centrifuge, and the centrifugal rotational speed that centrifuge is arranged is 4500~6000 turns/
Point, centrifugation extract clear liquid, then clear liquid is moved on Rotary Evaporators and does concentration, and be concentrated into volume five/
One obtains concentrate；
S3: backward S2 in concentrate in add 2.5 times of volumes dehydrated alcohol, and by its -80 DEG C at a temperature of
Overnight, the subsequent concentrate to after overnight, which revolving speed is arranged again is 6000 revs/min, carries out centrifugal treating again, and when centrifugation is arranged
Between be 30~60min, centrifuging temperature be 3~5 DEG C；
S4: the concentrate after being centrifuged in S3 is removed into clear liquid, 1ml dehydrated alcohol is added into concentrate, again to concentrate
After centrifugation 2~3 times, concentrate opening is placed, until dehydrated alcohol smell dissipates in standing concentrate, is prepared into okra glue
Matter polysaccharide.
Preferably, the time that enzymatic hydrolysis is arranged in the S1 is 120~140min, and the temperature setting digested is 45~60
DEG C, solid-liquid ratio is 1:48.5~1:49.8.
Preferably, leaching liquor is cooked and heats in the S1, and heating temperature is set as 98~100 DEG C, and heating time sets
It is set to 3~8min.
Preferably, the extracting mode of the protein is using α-amylase hydrolysis, and extracting solid-liquid ratio is 1:30, pH value
It is 55 DEG C for 10, setting Extracting temperature, setting extraction time is 90min, and the purifying in setose abelmoschus seed albumen uses ultrafiltration
Method, ultrafiltration membrane 5kDa.
Preferably, the flavone compound extracting mode uses ultrasonic-leaching method, and Extracting temperature is 40 DEG C, extracts
Solvent is 55% dehydrated alcohol, extraction time to be set as 30min, solid-liquid ratio 1:20.
The invention also provides a kind of Abelmoschus Esculentus Linn extracts to answer preparing the application in cosmetics, the Abelmoschus Esculentus Linn extract
Cosmetics for anti-oxidant, anti-aging, antifatigue enhancing skin elasticity.
Preferably, the Abelmoschus Esculentus Linn extract and cyclomethicone, ethyl alcohol, water, ethylhexyl salicylate, sweet
Oil, Magnus Petersson extract, methyl hydroxybenzoate, dimethyl silicone polymer, iso-butane are according to 4:1:1:2:1:1.5:1:0.5:0.3:0.4
The ratio of~6:3:2:4:2:3:1.2:0.8:0.5:0.7 is mixed into cosmetic composition, and cosmetic composition includes shield
Skin lotion, hand lotion, MASQUE APAISANT HYDRATANT, sunscreen spray and ultra light sun block lotion.
The beneficial effects of the present invention are:
1, of the invention, by being applied in skin protection cosmetics using the okra colloid polysaccharide in Abelmoschus Esculentus Linn extract, because
Okra colloid polysaccharide has a large amount of hydrophilic hydroxy groups, and the cosmetics being produced have good emulsibility, water imbibition and height viscous
Degree plays the role of improving the colour of skin, anti-aging and effective moisturizing.
2, of the invention, by preparing cosmetics using Abelmoschus Esculentus Linn extract, that is, utilize the okra in Abelmoschus Esculentus Linn extract
Colloid polysaccharide, fat, protein and flavone compound have the cosmetics containing Abelmoschus Esculentus Linn extract anti-oxidant, anti-ageing
Always, antifatigue, enhancing skin elasticity effect, and be produced as facial treatment milk, hand lotion, MASQUE APAISANT HYDRATANT, sunscreen spray and prevent
Lotion is shone, the effect of cosmetic skin beauty can be effectively played.
3, of the invention, for the extraction process of Abelmoschus Esculentus Linn extract, processing step is easy to operate, more using okra colloid
Sugar has good activity, convenient for being applied to the production and popularization of cosmetics.
Specific embodiment
Combined with specific embodiments below the present invention is made further to explain.
Embodiment one
A kind of Abelmoschus Esculentus Linn extract proposed by the present invention, including okra colloid polysaccharide, protein, fat and flavonoids
Close object；
The preparation method of the okra colloid polysaccharide the following steps are included:
S1: weighing 8g okra dry powder, and then choosing pH value is 6 food acid buffer as extracting solution, then weighs
Okra dry powder, extracting solution and cellulase, are then successively added in reactor tank, and set by cellulase of the 6g through overactivation
The time for setting enzymatic hydrolysis is 120min, the temperature of enzymatic hydrolysis is 45 DEG C, solid-liquid ratio is that 1:48.5 carries out constant temperature enzymatic hydrolysis, obtains okra
The leaching liquor of colloid polysaccharide, leaching liquor, which is cooked, to be heated, and heating temperature is set as 98 DEG C, and heating time is set as 3min；
S2: and then pour into the leaching liquor in S1 in centrifuge, the centrifugal rotational speed that centrifuge is arranged is 4500 revs/min, from
The heart extracts clear liquid, and then clear liquid is moved on Rotary Evaporators and does concentration, and be concentrated into 1/5th of volume
To concentrate；
S3: backward S2 in concentrate in add 2.5 times of volumes dehydrated alcohol, and by its -80 DEG C at a temperature of
Overnight, the subsequent concentrate to after overnight, which revolving speed is arranged again is 6000 revs/min, carries out centrifugal treating again, and when centrifugation is arranged
Between be 30min, centrifuging temperature be 3 DEG C；
S4: the concentrate after being centrifuged in S3 is removed into clear liquid, 1ml dehydrated alcohol is added into concentrate, again to concentrate
After centrifugation 2 times, concentrate opening is placed, until dehydrated alcohol smell dissipates in standing concentrate, is prepared into okra colloid
Polysaccharide.
S5: the Protein Extraction in okra in extract: the extracting mode of protein be using α-amylase hydrolysis, and
Extract that solid-liquid ratio is 1:30, pH value 10, setting Extracting temperature are 55 DEG C, setting extraction time is 90min, and setose abelmoschus seed
Purifying in albumen uses ultrafiltration, ultrafiltration membrane 5kDa.
S6: the flavone compound in okra in extract extracts: flavone compound extracting mode uses ultrasonic wave
Extraction, and Extracting temperature is 40 DEG C, Extraction solvent is 55% dehydrated alcohol, extraction time to be set as 30min, solid-liquid ratio 1:
20。
The invention also provides a kind of Abelmoschus Esculentus Linn extracts to be applied to preparing the application in cosmetics, Abelmoschus Esculentus Linn extract
Anti-oxidant, anti-aging, antifatigue, enhancing skin elasticity cosmetics.
Abelmoschus Esculentus Linn extract and cyclomethicone, ethyl alcohol, water, ethylhexyl salicylate, glycerol, Magnus Petersson are mentioned
Object, methyl hydroxybenzoate, dimethyl silicone polymer, iso-butane is taken to mix according to the ratio of 4:1:1:2:1:1.5:1:0.5:0.3:0.4
At cosmetic composition, and cosmetic composition includes facial treatment milk, hand lotion, MASQUE APAISANT HYDRATANT, sunscreen spray and sunscreen
Liquid.
Embodiment two
A kind of Abelmoschus Esculentus Linn extract proposed by the present invention, including okra colloid polysaccharide, protein, fat and flavonoids
Close object；
The preparation method of the okra colloid polysaccharide the following steps are included:
S1: weighing 10g okra dry powder, and then choosing pH value is 6 food acid buffer as extracting solution, then weighs
Okra dry powder, extracting solution and cellulase, are then successively added in reactor tank by cellulase of the 10g through overactivation, and
The time that enzymatic hydrolysis is arranged is 120min, the temperature of enzymatic hydrolysis is 45 DEG C, solid-liquid ratio is that 1:48.5 carries out constant temperature enzymatic hydrolysis, obtains Huang Qiu
The leaching liquor of certain herbaceous plants with big flowers colloid polysaccharide, leaching liquor, which is cooked, to be heated, and heating temperature is set as 98 DEG C, and heating time is set as 3min；
S2: and then pour into the leaching liquor in S1 in centrifuge, the centrifugal rotational speed that centrifuge is arranged is 4500 revs/min, from
The heart extracts clear liquid, and then clear liquid is moved on Rotary Evaporators and does concentration, and be concentrated into 1/5th of volume
To concentrate；
S3: backward S2 in concentrate in add 2.5 times of volumes dehydrated alcohol, and by its -80 DEG C at a temperature of
Overnight, the subsequent concentrate to after overnight, which revolving speed is arranged again is 6000 revs/min, carries out centrifugal treating again, and when centrifugation is arranged
Between be 30min, centrifuging temperature be 4 DEG C；
S4: the concentrate after being centrifuged in S3 is removed into clear liquid, 1ml dehydrated alcohol is added into concentrate, again to concentrate
After centrifugation 2 times, concentrate opening is placed, until dehydrated alcohol smell dissipates in standing concentrate, is prepared into okra colloid
Polysaccharide.
S5: the Protein Extraction in okra in extract: the extracting mode of protein be using α-amylase hydrolysis, and
Extract that solid-liquid ratio is 1:30, pH value 10, setting Extracting temperature are 55 DEG C, setting extraction time is 90min, and okra
Purifying in seed albumen uses ultrafiltration, ultrafiltration membrane 5kDa.
S6: the flavone compound in okra in extract extracts: flavone compound extracting mode uses ultrasonic wave
Extraction, and Extracting temperature is 40 DEG C, Extraction solvent is 55% dehydrated alcohol, extraction time to be set as 30min, solid-liquid ratio 1:
20。
The invention also provides a kind of Abelmoschus Esculentus Linn extracts to be applied to preparing the application in cosmetics, Abelmoschus Esculentus Linn extract
Anti-oxidant, anti-aging, antifatigue, enhancing skin elasticity cosmetics.
Abelmoschus Esculentus Linn extract and cyclomethicone, ethyl alcohol, water, ethylhexyl salicylate, glycerol, Magnus Petersson are mentioned
Take object, methyl hydroxybenzoate, dimethyl silicone polymer, iso-butane according to 4.5:1.2:1.5:2.5:1.5:1.6:1:0.5:0.3:0.4
Ratio be mixed into cosmetic composition, and cosmetic composition includes facial treatment milk, hand lotion, MASQUE APAISANT HYDRATANT, sun-proof spray
Mist and ultra light sun block lotion.
Embodiment three
A kind of Abelmoschus Esculentus Linn extract proposed by the present invention, including okra colloid polysaccharide, protein, fat and flavonoids
Close object；
The preparation method of the okra colloid polysaccharide the following steps are included:
S1: weighing 12g okra dry powder, and then choosing pH value is 6 food acid buffer as extracting solution, then weighs
Okra dry powder, extracting solution and cellulase, are then successively added in reactor tank by cellulase of the 10g through overactivation, and
The time that enzymatic hydrolysis is arranged is 130min, the temperature of enzymatic hydrolysis is 50 DEG C, solid-liquid ratio is that 1:49 carries out constant temperature enzymatic hydrolysis, obtains okra
The leaching liquor of colloid polysaccharide, leaching liquor, which is cooked, to be heated, and heating temperature is set as 100 DEG C, and heating time is set as 6min；
S2: and then pour into the leaching liquor in S1 in centrifuge, the centrifugal rotational speed that centrifuge is arranged is 5000 revs/min, from
The heart extracts clear liquid, and then clear liquid is moved on Rotary Evaporators and does concentration, and be concentrated into 1/5th of volume
To concentrate；
S3: backward S2 in concentrate in add 2.5 times of volumes dehydrated alcohol, and by its -80 DEG C at a temperature of
Overnight, the subsequent concentrate to after overnight, which revolving speed is arranged again is 6000 revs/min, carries out centrifugal treating again, and when centrifugation is arranged
Between be 50min, centrifuging temperature be 4 DEG C；
S4: the concentrate after being centrifuged in S3 is removed into clear liquid, 1ml dehydrated alcohol is added into concentrate, again to concentrate
After centrifugation 3 times, concentrate opening is placed, until dehydrated alcohol smell dissipates in standing concentrate, is prepared into okra colloid
Polysaccharide.
S5: the Protein Extraction in okra in extract: the extracting mode of protein be using α-amylase hydrolysis, and
Extract that solid-liquid ratio is 1:30, pH value 10, setting Extracting temperature are 55 DEG C, setting extraction time is 90min, and okra
Purifying in seed albumen uses ultrafiltration, ultrafiltration membrane 5kDa.
S6: the flavone compound in okra in extract extracts: flavone compound extracting mode uses ultrasonic wave
Extraction, and Extracting temperature is 40 DEG C, Extraction solvent is 55% dehydrated alcohol, extraction time to be set as 30min, solid-liquid ratio 1:
20。
The invention also provides a kind of Abelmoschus Esculentus Linn extracts to be applied to preparing the application in cosmetics, Abelmoschus Esculentus Linn extract
Anti-oxidant, anti-aging, antifatigue, enhancing skin elasticity cosmetics.
Abelmoschus Esculentus Linn extract and cyclomethicone, ethyl alcohol, water, ethylhexyl salicylate, glycerol, Magnus Petersson are mentioned
Take object, methyl hydroxybenzoate, dimethyl silicone polymer, iso-butane according to 5:1.5:1.5:3:1.5:2:1:0.6:0.4:0.6 ratio
It is mixed into cosmetic composition, and cosmetic composition includes facial treatment milk, hand lotion, MASQUE APAISANT HYDRATANT, sunscreen spray and prevents
Shine lotion.
Example IV
A kind of Abelmoschus Esculentus Linn extract proposed by the present invention, including okra colloid polysaccharide, protein, fat and flavonoids
Close object；
The preparation method of the okra colloid polysaccharide the following steps are included:
S1: weighing 12g okra dry powder, and then choosing pH value is 6 food acid buffer as extracting solution, then weighs
Okra dry powder, extracting solution and cellulase, are then successively added in reactor tank by cellulase of the 10g through overactivation, and
The time that enzymatic hydrolysis is arranged is 135min, the temperature of enzymatic hydrolysis is 50 DEG C, solid-liquid ratio is that 1:49.5 carries out constant temperature enzymatic hydrolysis, obtains Huang Qiu
The leaching liquor of certain herbaceous plants with big flowers colloid polysaccharide, leaching liquor, which is cooked, to be heated, and heating temperature is set as 100 DEG C, and heating time is set as
7min；
S2: and then pour into the leaching liquor in S1 in centrifuge, the centrifugal rotational speed that centrifuge is arranged is 5000 revs/min, from
The heart extracts clear liquid, and then clear liquid is moved on Rotary Evaporators and does concentration, and be concentrated into 1/5th of volume
To concentrate；
S3: backward S2 in concentrate in add 2.5 times of volumes dehydrated alcohol, and by its -80 DEG C at a temperature of
Overnight, the subsequent concentrate to after overnight, which revolving speed is arranged again is 6000 revs/min, carries out centrifugal treating again, and when centrifugation is arranged
Between be 60min, centrifuging temperature be 5 DEG C；
S4: the concentrate after being centrifuged in S3 is removed into clear liquid, 1ml dehydrated alcohol is added into concentrate, again to concentrate
After centrifugation 3 times, concentrate opening is placed, until dehydrated alcohol smell dissipates in standing concentrate, is prepared into okra colloid
Polysaccharide.
S5: the Protein Extraction in okra in extract: the extracting mode of protein be using α-amylase hydrolysis, and
Extract that solid-liquid ratio is 1:30, pH value 10, setting Extracting temperature are 55 DEG C, setting extraction time is 90min, and okra
Purifying in seed albumen uses ultrafiltration, ultrafiltration membrane 5kDa.
S6: the flavone compound in okra in extract extracts: flavone compound extracting mode uses ultrasonic method,
And Extracting temperature is 40 DEG C, Extraction solvent is 55% dehydrated alcohol, extraction time to be set as 30min, solid-liquid ratio 1:20.
The invention also provides a kind of Abelmoschus Esculentus Linn extracts to be applied to preparing the application in cosmetics, Abelmoschus Esculentus Linn extract
Anti-oxidant, anti-aging, antifatigue, enhancing skin elasticity cosmetics.
Abelmoschus Esculentus Linn extract and cyclomethicone, ethyl alcohol, water, ethylhexyl salicylate, glycerol, Magnus Petersson are mentioned
Take object, methyl hydroxybenzoate, dimethyl silicone polymer, iso-butane according to 5.5:2.5:1.5:3.5:1.5:2.5:1:0.6:0.4:0.6
Ratio be mixed into cosmetic composition, and cosmetic composition includes facial treatment milk, hand lotion, MASQUE APAISANT HYDRATANT, sun-proof spray
Mist and ultra light sun block lotion.
Embodiment five
A kind of Abelmoschus Esculentus Linn extract proposed by the present invention, including okra colloid polysaccharide, protein, fat and flavonoids
Close object；
The preparation method of the okra colloid polysaccharide the following steps are included:
S1: weighing 15g okra dry powder, and then choosing pH value is 6 food acid buffer as extracting solution, then weighs
Okra dry powder, extracting solution and cellulase, are then successively added in reactor tank by cellulase of the 12g through overactivation, and
The time that enzymatic hydrolysis is arranged is 140min, the temperature of enzymatic hydrolysis is 60 DEG C, solid-liquid ratio is that 1:49.8 carries out constant temperature enzymatic hydrolysis, obtains Huang Qiu
The leaching liquor of certain herbaceous plants with big flowers colloid polysaccharide, leaching liquor, which is cooked, to be heated, and heating temperature is set as 100 DEG C, and heating time is set as
8min；
S2: and then pour into the leaching liquor in S1 in centrifuge, the centrifugal rotational speed that centrifuge is arranged is 6000 revs/min, from
The heart extracts clear liquid, and then clear liquid is moved on Rotary Evaporators and does concentration, and be concentrated into 1/5th of volume
To concentrate；
S3: backward S2 in concentrate in add 2.5 times of volumes dehydrated alcohol, and by its -80 DEG C at a temperature of
Overnight, the subsequent concentrate to after overnight, which revolving speed is arranged again is 6000 revs/min, carries out centrifugal treating again, and when centrifugation is arranged
Between be 60min, centrifuging temperature be 5 DEG C；
S4: the concentrate after being centrifuged in S3 is removed into clear liquid, 1ml dehydrated alcohol is added into concentrate, again to concentrate
After centrifugation 3 times, concentrate opening is placed, until dehydrated alcohol smell dissipates in standing concentrate, is prepared into okra colloid
Polysaccharide.
S5: the Protein Extraction in okra in extract: the extracting mode of protein be using α-amylase hydrolysis, and
Extract that solid-liquid ratio is 1:30, pH value 10, setting Extracting temperature are 55 DEG C, setting extraction time is 90min, and okra
Purifying in seed albumen uses ultrafiltration, ultrafiltration membrane 5kDa.
S6: the flavone compound in okra in extract extracts: flavone compound extracting mode uses ultrasonic wave
Extraction, and Extracting temperature is 40 DEG C, Extraction solvent is 55% dehydrated alcohol, extraction time to be set as 30min, solid-liquid ratio 1:
20。
The invention also provides a kind of Abelmoschus Esculentus Linn extracts to be applied to preparing the application in cosmetics, Abelmoschus Esculentus Linn extract
Anti-oxidant, anti-aging, antifatigue, enhancing skin elasticity cosmetics.
Abelmoschus Esculentus Linn extract and cyclomethicone, ethyl alcohol, water, ethylhexyl salicylate, glycerol, Magnus Petersson are mentioned
Object, methyl hydroxybenzoate, dimethyl silicone polymer, iso-butane is taken to mix according to the ratio of 6:3:2:4:2:3:1.2:0.8:0.5:0.7
At cosmetic composition, and cosmetic composition includes facial treatment milk, hand lotion, MASQUE APAISANT HYDRATANT, sunscreen spray and sunscreen
Liquid.
Abelmoschus Esculentus Linn extract prepared by embodiment five is arrived to above-described embodiment one, to the okra glue in Abelmoschus Esculentus Linn extract
Matter polysaccharide, protein, fat and flavone compound mass percent concentration detected, it is as a result as follows:
It is carried out in preparation process according to above-described embodiment one to embodiment five to Abelmoschus Esculentus Linn extract, to okra colloid
Centrifugation number, centrifugation time and the centrifuging temperature of polysaccharide, it is right to okra colloid polysaccharide concentration quality there are certain influence
Added by Abelmoschus Esculentus Linn extract and prepare the centrifugation purity presence influence that cosmetics use, measurement result is as follows:
| It is centrifuged number | Centrifugation time | Centrifuging temperature | It is centrifuged purity | |
| Embodiment one | 4 times | 30min | 3℃ | It is lower |
| Embodiment two | 4 times | 30min | 3℃ | It is higher |
| Embodiment three | 5 times | 50min | 4℃ | It is high |
| Example IV | 5 times | 60min | 5℃ | It is higher |
| Embodiment five | 5 times | 60min | 5℃ | It is higher |
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (7)
1. a kind of Abelmoschus Esculentus Linn extract, including okra colloid polysaccharide, protein, fat and flavone compound；
The preparation method of the okra colloid polysaccharide the following steps are included:
S1: weighing 8~15g okra dry powder, and then choosing pH value is 6 food acid buffer as extracting solution, then weighs 6
Okra dry powder, extracting solution and cellulase, are then successively added in reactor tank by cellulase of~the 12g through overactivation,
And the time of enzymatic hydrolysis, the temperature of enzymatic hydrolysis, solid-liquid ratio progress constant temperature enzymatic hydrolysis are set, obtain the leaching liquor of okra colloid polysaccharide；
S2: and then pour into the leaching liquor in S1 in centrifuge, the centrifugal rotational speed that centrifuge is arranged is 4500~6000 revs/min,
Centrifugation extracts clear liquid, and then clear liquid is moved on Rotary Evaporators and does concentration, and is concentrated into 1/5th of volume
Obtain concentrate；
S3: backward S2 in concentrate in add 2.5 times of volumes dehydrated alcohol, and by its -80 DEG C at a temperature of overnight,
It is 6000 revs/min of progress centrifugal treating again that revolving speed, which is arranged, in the subsequent concentrate to after staying overnight again, and it is 30 that centrifugation time, which is arranged,
~60min, centrifuging temperature are 3~5 DEG C；
S4: removing clear liquid for the concentrate after being centrifuged in S3,1ml dehydrated alcohol added into concentrate, again to concentrate centrifugation 2
After~3 times, concentrate opening is placed, until dehydrated alcohol smell dissipates in standing concentrate, it is more to be prepared into okra colloid
Sugar.
2. a kind of Abelmoschus Esculentus Linn extract according to claim 1, which is characterized in that the time that enzymatic hydrolysis is arranged in the S1 is
120~140min, and the temperature setting digested is 45~60 DEG C, solid-liquid ratio is 1:48.5~1:49.8.
3. a kind of Abelmoschus Esculentus Linn extract according to claim 1, which is characterized in that leaching liquor is cooked at heating in the S1
Reason, and heating temperature is set as 98~100 DEG C, heating time is set as 3~8min.
4. a kind of Abelmoschus Esculentus Linn extract according to claim 1, which is characterized in that the extracting mode of the protein is to adopt
With α-amylase hydrolysis, and extract that solid-liquid ratio is 1:30, pH value 10, setting Extracting temperature are 55 DEG C, setting extraction time is
90min, and the purifying in setose abelmoschus seed albumen uses ultrafiltration, ultrafiltration membrane 5kDa.
5. a kind of Abelmoschus Esculentus Linn extract according to claim 1, which is characterized in that the flavone compound extracting mode
Using ultrasonic-leaching method, and Extracting temperature is 40 DEG C, Extraction solvent be set as 55% dehydrated alcohol, extraction time 30min,
Solid-liquid ratio is 1:20.
6. a kind of Abelmoschus Esculentus Linn extract is preparing the application in cosmetics, which is characterized in that the Abelmoschus Esculentus Linn extract is applied to
Anti-oxidant, anti-aging, antifatigue, enhancing skin elasticity cosmetics.
7. a kind of Abelmoschus Esculentus Linn extract according to claim 6 is preparing the application in cosmetics, which is characterized in that described
Abelmoschus Esculentus Linn extract and cyclomethicone, ethyl alcohol, water, ethylhexyl salicylate, glycerol, Magnus Petersson extract, oxybenzene
Methyl esters, dimethyl silicone polymer, iso-butane are according to 4:1:1:2:1:1.5:1:0.5:0.3:0.4~6:3:2:4:2:3:1.2:
The ratio of 0.8:0.5:0.7 is mixed into cosmetic composition, and cosmetic composition includes facial treatment milk, hand lotion, moisturizing
Facial mask, sunscreen spray and ultra light sun block lotion.
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Application publication date: 20181207 |