CN105943467A - Method for extracting natural humectant from abelmoschus manihot - Google Patents
Method for extracting natural humectant from abelmoschus manihot Download PDFInfo
- Publication number
- CN105943467A CN105943467A CN201610490300.1A CN201610490300A CN105943467A CN 105943467 A CN105943467 A CN 105943467A CN 201610490300 A CN201610490300 A CN 201610490300A CN 105943467 A CN105943467 A CN 105943467A
- Authority
- CN
- China
- Prior art keywords
- abelmoschus esculentus
- alcohol
- moisture preserving
- natural moisture
- preserving agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Dermatology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Cosmetics (AREA)
Abstract
The invention relates to a method for extracting a natural humectant from abelmoschus manihot. The method comprises the following steps: (1), performing crushing treatment on the abelmoschus manihot; (2), adding disodium hydrogen phosphate-citrate buffer solution into the crushed abelmoschus manihot, and adopting microwave extraction; (3), performing centrifugal separation on extracting solution, and taking liquid in the upper layer; (4), concentrating the liquid in the upper layer to obtain concentrated solution, adding ethyl alcohol into the concentrated solution, and performing alcohol precipitation; (5), performing centrifugation on alcohol deposit fluid, and washing colloid in the lower layer through ethyl alcohol and collecting the colloid, so as to obtain the abelmoschus manihot natural humectants. Compared with the prior art, the method has the advantages that the extracting speed is high, and extracted products can serve as a moisturizing additive of cosmetics and have the characteristics of green, environment protection and good moisturizing effect, thereby having a wide application prospect.
Description
Technical field
The invention belongs to the present invention relates to household chemicals field, especially relate to one from Abelmoschus esculentus, extract natural guarantor
The method of humectant.
Background technology
Abelmoschus esculentus rich in nutrition content, rich in proteins, unsaturated fatty acid, vitamin, polysaccharide, flavone
Compounds etc., be a kind of nutritive health-care vegetable [Ren Dandan, Chen Gu. Food Science, 2010,31 (21):
353-356].Research proves that Abelmoschus esculentus has anticancer, reduction blood fat, resisting fatigue, the elasticity of enhancing skin,
The effects such as minimizing liver injury of lung [Wang Jianrui. University Of Agriculture and Forestry In Fujian's master thesis, 2013].The particularly yellow autumn
Containing abundant mucosubstance in the tender pod of certain herbaceous plants with big flowers, mainly by arabinan, galactan, Pectic polysaccharides
And the mixture that glycoprotein forms on a small quantity, there is the effect of eliminating toxin and beautifying the skin, reduce toxin and deposit in vivo, protect
Water holding grades effect.
The method of the extracts such as existing extraction Abelmoschus esculentus polysaccharide is mainly traditional water extraction and supersound extraction, carries
Take inefficient, due to long-time high temperature, the quality of extract also relative drop.Therefore, in the urgent need to sending out
Open up more efficient extracting method.
Multistage microwave amplifier technique is simple, selectivity is strong, the time is short, solvent load is little, be heated evenly, target
Component yield is high.And there are some researches prove that the extraction ratio of microwaved rear pectin improves, the pectic substance obtained
Measure, esterification degree is high, molecular weight is big, gel strength is big [Liu Xiaoxia. Zhejiang University's master thesis, 2014].
Therefore for the deficiency in terms of present Abelmoschus esculentus extraction, microwave extraction natural moisture preserving agent, pectin etc. are selected to extract
The quality of thing all increases, and has important practical significance on producing.And existing Abelmoschus Esculentus Linn extract
Being mainly used in the industry such as food and medicine, it is the most effectively developed in the application of cosmetic industry.Cause
This, a kind of method extracting natural moisture preserving agent from Abelmoschus esculentus has important practical significance.
Summary of the invention
Defect that the purpose of the present invention is contemplated to overcome above-mentioned prior art to exist and provide a kind of from the yellow autumn
The method extracting natural moisture preserving agent in certain herbaceous plants with big flowers, the inventive method has the feature of green, safety, low cost, tool
Have wide practical use.
The purpose of the present invention can be achieved through the following technical solutions:
A kind of method extracting natural moisture preserving agent from Abelmoschus esculentus, the method comprises the following steps:
(1) Abelmoschus esculentus is carried out break process；
(2) Abelmoschus esculentus after crushing adds disodium hydrogen phosphate-citrate buffer solution, and uses microwave extraction；
(3) it is centrifuged extracting solution separating, takes supernatant liquid；
(4) supernatant liquid is concentrated to give concentrated solution, concentrated solution adds ethanol, carries out alcohol precipitation；
(5) alcohol deposit fluid is centrifuged, the colloid of lower floor, by washing with alcohol, collects, i.e. obtain Abelmoschus esculentus sky
So wetting agent.
The Abelmoschus esculentus of step (1) break process is fresh Abelmoschus esculentus, and uses deionization before break process
Water is cleaned.
In step (2), the operating condition of microwave extraction is: Abelmoschus esculentus and disodium hydrogen phosphate-citrate buffer solution
Mass volume ratio be 1g:3-10mL, the power of microwave is 320-640W, and extraction time is 1-3 time,
Extraction time is 1-3min every time.
The pH value of described disodium hydrogen phosphate-citrate buffer solution is 2-5.
Step (4) liquid at the middle and upper levels is 3-10:1 with the volume ratio of concentrated solution.
The amount of alcohol added in step (4) is 2-5:1 with the volume ratio of concentrated solution.
The volume fraction of ethanol added in step (4) is 95%.
Compared with prior art, this method extraction rate is fast, and the product of extraction can be as the moisturizing of cosmetics
Additive, has the features such as green, environmental protection, moistening effect be good, is therefore with a wide range of applications.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in detail.
Embodiment 1
(1) take Abelmoschus esculentus fresh for 10g to be washed with deionized water totally, smash to pieces with juice extractor；
(2) the Abelmoschus esculentus slurry smashed to pieces adds 50mL disodium hydrogen phosphate-citrate buffer solution, and (pH value is
3.0), build with preservative film, put it into and microwave oven processes 3min (microwave power is 640W), 15
After min, again by same condition microwave extraction once.
(3) Abelmoschus esculentus extracting solution centrifuge derived above, takes supernatant liquid, and lower floor's solid discards；
(4) centrifugal obtain upper liquid Rotary Evaporators and concentrate, be concentrated into 10mL, be subsequently adding
95% ethanol of 30mL, carries out alcohol precipitation, static 12h；
(5) alcohol deposit fluid is centrifuged, the colloid of lower floor, respectively with 5mL 75% ethanol and absolute ethanol washing,
Collect, i.e. obtain the Abelmoschus esculentus natural moisture preserving agent of 3.1g.
Embodiment 2
(1) take Abelmoschus esculentus fresh for 10g to be washed with deionized water totally, smash to pieces with juice extractor；
(2) the Abelmoschus esculentus slurry smashed to pieces adds 80mL disodium hydrogen phosphate-citrate buffer solution, and (pH value is
2.0), build with preservative film, put it into and microwave oven processes 2min (microwave power is 640W), 15
After min, again by same condition microwave extraction once.
(3) Abelmoschus esculentus extracting solution centrifuge derived above, takes supernatant liquid, and lower floor's solid discards；
(4) centrifugal obtain upper liquid Rotary Evaporators and concentrate, be concentrated into 15mL, be subsequently adding 40
95% ethanol of mL, carries out alcohol precipitation, static 12h；
(5) alcohol deposit fluid is centrifuged, the colloid of lower floor, respectively with 5mL 75% ethanol and absolute ethanol washing,
Collect, i.e. obtain the Abelmoschus esculentus natural moisture preserving agent of 2.9g.
Embodiment 3
A kind of method extracting natural moisture preserving agent from Abelmoschus esculentus, the method comprises the following steps:
(1) fresh Abelmoschus esculentus is washed with deionized water totally, smashs to pieces with juice extractor；
(2) Abelmoschus esculentus after crushing adds disodium hydrogen phosphate-citrate buffer solution, and uses microwave extraction,
Operating condition is: Abelmoschus esculentus is 1g:3mL with the mass volume ratio of disodium hydrogen phosphate-citrate buffer solution, micro-
The power of ripple is 320W, and extraction time is 1 time, and each extraction time is 3min, disodium hydrogen phosphate-Fructus Citri Limoniae
The pH value of acid buffer is 2；
(3) it is centrifuged extracting solution separating, takes supernatant liquid；
(4) supernatant liquid being concentrated to give concentrated solution, supernatant liquid is 3:1 with the volume ratio of concentrated solution,
Adding ethanol in concentrated solution, amount of alcohol is 2:1 with the volume ratio of concentrated solution, the volume fraction of ethanol of addition
It is 95%, carries out alcohol precipitation；
(5) alcohol deposit fluid is centrifuged, the colloid of lower floor, by washing with alcohol, collects, i.e. obtain Abelmoschus esculentus sky
So wetting agent.
Embodiment 4
A kind of method extracting natural moisture preserving agent from Abelmoschus esculentus, the method comprises the following steps:
(1) fresh Abelmoschus esculentus is washed with deionized water totally, smashs to pieces with juice extractor；
(2) Abelmoschus esculentus after crushing adds disodium hydrogen phosphate-citrate buffer solution, and uses microwave extraction,
Operating condition is: Abelmoschus esculentus is 1g:7mL with the mass volume ratio of disodium hydrogen phosphate-citrate buffer solution, micro-
The power of ripple is 540W, and extraction time is 2 times, and each extraction time is 2min, disodium hydrogen phosphate-Fructus Citri Limoniae
The pH value of acid buffer is 3；
(3) it is centrifuged extracting solution separating, takes supernatant liquid；
(4) supernatant liquid being concentrated to give concentrated solution, supernatant liquid is 7:1 with the volume ratio of concentrated solution,
Adding ethanol in concentrated solution, amount of alcohol is 4:1 with the volume ratio of concentrated solution, the volume fraction of ethanol of addition
It is 95%, carries out alcohol precipitation；
(5) alcohol deposit fluid is centrifuged, the colloid of lower floor, by washing with alcohol, collects, i.e. obtain Abelmoschus esculentus sky
So wetting agent.
Embodiment 5
A kind of method extracting natural moisture preserving agent from Abelmoschus esculentus, the method comprises the following steps:
(1) fresh Abelmoschus esculentus is washed with deionized water totally, smashs to pieces with juice extractor；
(2) Abelmoschus esculentus after crushing adds disodium hydrogen phosphate-citrate buffer solution, and uses microwave extraction,
Operating condition is: Abelmoschus esculentus is 1g:10mL with the mass volume ratio of disodium hydrogen phosphate-citrate buffer solution,
The power of microwave is 640W, and extraction time is 3 times, and each extraction time is 1min, disodium hydrogen phosphate-
The pH value of citrate buffer solution is 5；
(3) it is centrifuged extracting solution separating, takes supernatant liquid；
(4) supernatant liquid being concentrated to give concentrated solution, supernatant liquid is 10:1 with the volume ratio of concentrated solution,
Adding ethanol in concentrated solution, amount of alcohol is 5:1 with the volume ratio of concentrated solution, the volume fraction of ethanol of addition
It is 95%, carries out alcohol precipitation；
(5) alcohol deposit fluid is centrifuged, the colloid of lower floor, by washing with alcohol, collects, i.e. obtain Abelmoschus esculentus sky
So wetting agent.
The above-mentioned description to embodiment is to be understood that for ease of those skilled in the art and make
With invention.These embodiments obviously easily can be made various amendment by person skilled in the art,
And General Principle described herein is applied in other embodiments without through performing creative labour.Cause
This, the invention is not restricted to above-described embodiment, and those skilled in the art are according to the announcement of the present invention, without departing from this
Improvement and amendment that invention category is made all should be within protection scope of the present invention.
Claims (7)
1. one kind from Abelmoschus esculentus extract natural moisture preserving agent method, it is characterised in that the method include with
Lower step:
(1) Abelmoschus esculentus is carried out break process；
(2) Abelmoschus esculentus after crushing adds disodium hydrogen phosphate-citrate buffer solution, and uses microwave extraction；
(3) it is centrifuged extracting solution separating, takes supernatant liquid；
(4) supernatant liquid is concentrated to give concentrated solution, concentrated solution adds ethanol, carries out alcohol precipitation；
(5) alcohol deposit fluid is centrifuged, the colloid of lower floor, by washing with alcohol, collects, i.e. obtain Abelmoschus esculentus sky
So wetting agent.
A kind of method extracting natural moisture preserving agent from Abelmoschus esculentus the most according to claim 1, it is special
Levying and be, the Abelmoschus esculentus of step (1) break process is fresh Abelmoschus esculentus, and spends before break process
Ionized water is cleaned.
A kind of method extracting natural moisture preserving agent from Abelmoschus esculentus the most according to claim 1, it is characterised in that
In step (2), the operating condition of microwave extraction is: Abelmoschus esculentus and the quality volume of disodium hydrogen phosphate-citrate buffer solution
Than being 1g:3-10mL, the power of microwave is 320-640W, and extraction time is 1-3 time, and each extraction time is
1-3min。
A kind of method extracting natural moisture preserving agent from Abelmoschus esculentus the most according to claim 1 and 2, its feature
Being, the pH value of described disodium hydrogen phosphate-citrate buffer solution is 2-5.
A kind of method extracting natural moisture preserving agent from Abelmoschus esculentus the most according to claim 1, it is characterised in that
Step (4) liquid at the middle and upper levels is 3-10:1 with the volume ratio of concentrated solution.
A kind of method extracting natural moisture preserving agent from Abelmoschus esculentus the most according to claim 1, it is characterised in that
The amount of alcohol added in step (4) is 2-5:1 with the volume ratio of concentrated solution.
A kind of method extracting natural moisture preserving agent from Abelmoschus esculentus the most according to claim 1, it is characterised in that
The volume fraction of ethanol added in step (4) is 95%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610490300.1A CN105943467A (en) | 2016-06-27 | 2016-06-27 | Method for extracting natural humectant from abelmoschus manihot |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610490300.1A CN105943467A (en) | 2016-06-27 | 2016-06-27 | Method for extracting natural humectant from abelmoschus manihot |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105943467A true CN105943467A (en) | 2016-09-21 |
Family
ID=56902916
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610490300.1A Pending CN105943467A (en) | 2016-06-27 | 2016-06-27 | Method for extracting natural humectant from abelmoschus manihot |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105943467A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107811925A (en) * | 2017-12-24 | 2018-03-20 | 姚佑灿 | A kind of water moistens net face mildy wash |
| CN108938503A (en) * | 2018-09-29 | 2018-12-07 | 广州今盛美精细化工有限公司 | A kind of Abelmoschus Esculentus Linn extract and its preparing the application in cosmetics |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102399303A (en) * | 2011-10-31 | 2012-04-04 | 牛倩 | Extraction method of pectin |
| CN102993331A (en) * | 2013-01-06 | 2013-03-27 | 湖南奇异生物科技有限公司 | Method for extracting okra dried fruit pectins |
-
2016
- 2016-06-27 CN CN201610490300.1A patent/CN105943467A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102399303A (en) * | 2011-10-31 | 2012-04-04 | 牛倩 | Extraction method of pectin |
| CN102993331A (en) * | 2013-01-06 | 2013-03-27 | 湖南奇异生物科技有限公司 | Method for extracting okra dried fruit pectins |
Non-Patent Citations (4)
| Title |
|---|
| 刘娜: "新兴蔬菜黄秋葵果胶提取工艺", 《食品工程》 * |
| 张娜: "黄秋葵果胶的提取工艺研究", 《安徽农业科学》 * |
| 朱校奇等: "《药食兼用植物栽培技术》", 30 September 2013 * |
| 黄梓琪等: "微波辅助草酸铵提取黄秋葵果胶的工艺研究", 《广州化工》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107811925A (en) * | 2017-12-24 | 2018-03-20 | 姚佑灿 | A kind of water moistens net face mildy wash |
| CN108938503A (en) * | 2018-09-29 | 2018-12-07 | 广州今盛美精细化工有限公司 | A kind of Abelmoschus Esculentus Linn extract and its preparing the application in cosmetics |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101914304B (en) | Method for extracting blueberry anthocyanin | |
| CN107488515B (en) | Extracting solution and method for extracting tea tree flower essential oil by using same | |
| CN105669800B (en) | A kind of combined extracting essential oil from citrus, pectin, aurantiamarin, the method for synephrine and limonin | |
| CN104277915A (en) | Method for extracting peony essential oil from peony | |
| CN103145872B (en) | Panax notoginseng polysaccharide with low ash content and preparation method of polysaccharide | |
| CN101249060A (en) | Multiple-effect washing cream and method of preparing the same | |
| WO2010009667A1 (en) | Method for producing an extract of vitis vinifera having high orac values and the extract produced by said method | |
| CN101891580A (en) | Method for supercritical carbon dioxide extraction of lycopene in watermelon | |
| CN104403014A (en) | High yield preparation method of grifola frondosa polysaccharide | |
| CN109180825A (en) | Pilose antler mushroom polyoses extract and the preparation method and application thereof | |
| CN107573317A (en) | A kind of extracting method of blueberry anthocyanin | |
| CN106692211A (en) | Preparation method of taiwanofungus camphorates mycelium triterpene extract | |
| CN106344429A (en) | Flammulina velutipes essence and preparation method thereof | |
| CN105943467A (en) | Method for extracting natural humectant from abelmoschus manihot | |
| CN107459587A (en) | A kind of method that water-soluble polysaccharide is extracted from fruit body of edible fungi | |
| CN102551170A (en) | High efficiency preparation method of burdock leaf natural antioxidant | |
| CN106866835B (en) | A kind of ultrasonic technology extracts the preparation method of bletilla striata extract | |
| CN103980730A (en) | Preparation method for high-purity pitaya pigment | |
| CN106236667A (en) | A kind of P. delavayi natural skin care product and preparation method thereof | |
| CN104761655A (en) | Method for extracting sea mushroom polysaccharide from sea mushroom leftovers | |
| CN106187976A (en) | A kind of method extracting Rhizoma Steudnerae Henryanae procyanidins | |
| CN106924398A (en) | Longan seed polyphenol extracting method | |
| CN106753912A (en) | A kind of shinyleaf yellowhorn oil handmade soap and preparation method thereof | |
| CN103751061B (en) | Tomato leaf extract and the application in cosmetics | |
| CN110236172A (en) | A kind of food seaweed algae salt production process |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160921 |