CN106344429A

CN106344429A - Flammulina velutipes essence and preparation method thereof

Info

Publication number: CN106344429A
Application number: CN201610866005.1A
Authority: CN
Inventors: 董娟娥; 梁宗锁; 刘景煜; 李晨; 肖林刚
Original assignee: Northwest A&F University
Current assignee: Northwest A&F University
Priority date: 2016-09-29
Filing date: 2016-09-29
Publication date: 2017-01-25
Anticipated expiration: 2036-09-29
Also published as: CN106344429B

Abstract

The invention relates to flammulina velutipes essence and a preparation method thereof. A formula of the flammulina velutipes essence comprises flammulina velutipes polysaccharides, glycerine, 1,2-propylene glycol, sodium hyaluronate, trehalose, citric acid, D-panthenol, nicotinamide, a stabilizing agent, a preservative and water. The flammulina velutipes essence prepared by the preparation method takes the flammulina velutipes polysaccharides as a core component, and has the beauty maintaining and young keeping effects, such as preserving moisture, resisting oxidation, resisting aging and compacting skin; the contained sodium hyaluronate is a natural high moisturizing factor, and the components, such as the trehalose, the nicotinamide and the D-panthenol, are supplemented, so that the flammulina velutipes essence has the effects of highly moisturizing, whitening and compacting the skin, and can also keep the cell viability and improve the anti-adversity ability of the skin in severe environments, such as high temperature, high cold and intensive ultraviolet radiation.

Description

A kind of Flammulina velutiper (Fr.) Sing essence and preparation method thereof

Technical field

The invention belongs to cosmetic technical field is and in particular to a kind of Flammulina velutiper (Fr.) Sing essence and preparation method thereof.

Background technology

Flammulina velutiper (Fr.) Sing [flammulina velutipes (curt fr) sing.] is load Zoopagales money Pseudomonas, is a kind of Medicine-food two-purpose bacterium.Main component has polysaccharide, albumen, flavone, polyphenol and aminoacid etc..Wherein, polysaccharide is as main effective one-tenth / mono-, there is the pharmacological actions such as antitumor, antioxidation, enhance immunity.

From the point of view of medical angle, the skin of health should have the feature that smooth tender, fine and transparent, moist flawless, High resilience.Many factors in living environment, the such as increase of social pressure, irregular, living environment pollution of diet etc. Skin histology underactivity can be led to, obstacle in cell metabolism, microcirculation, and then present pachylosiies, drying, dim Light, lack the phenomenons such as gloss, wrinkle, pigmentation.

Common astringent has cosmetic water, toner/smoothing toner, astringent lotion etc., and the Nutrition of essence is higher than common astringent, It can releive tight, be dried skin.Essence is typically free of ethanol, non-stimulated to skin, more preferably absorbs nutrition.Essence Effective ingredient is not only merely resting on skin surface, can also reach the deep layer of skin, directly participates in the metabolism of cell, Skin can be allowed to reply water profit state in moment.

Content of the invention

For defect of the prior art and deficiency, the purpose of the present invention be exploitation one kind can for a long time moisturizing, holding flesh Skin cytoactive simultaneously has the essence of whitening, careful skin effect it is proposed that a kind of Flammulina velutiper (Fr.) Sing essence and preparation method thereof, This essence not stimulating component such as spirituosity, raw materials used safety non-toxic, good with skin affinity, and preparation method is simple, Green safety, is suitable for a large amount of production.

For reaching above-mentioned purpose, the technical scheme that the present invention takes is:

A kind of Flammulina velutiper (Fr.) Sing essence, including Flammukinan, glycerol, 1,2-PD, hyaluronate sodium, trehalose, lemon Lemon acid, d- pantothenylol, nicotiamide, stabilizer, preservative and water.

Specifically, according to the mass fraction, including 0.05～0.25 part of Flammukinan, 1～10 part of glycerol, 1,2-PD 0.5～1.5 part, 0.1～0.5 part of hyaluronate sodium, 0.5～2 part of trehalose, 0.05～0.2 part of citric acid, d- pantothenylol 0.5～2 Part, 0.5～2 part of nicotiamide, 0.2～0.5 part of stabilizer, 0.05 part of preservative, balance of water, the total amount of mentioned component is 100 Part.

More specifically, described Flammukinan is that slightly to be raised Flammukinan using water extraction to Flammulina velutiper (Fr.) Sing thick Extract, then by aqueous two-phase system, Flammukinan crude extract is carried out Flammukinan is obtained by extraction；Described aqueous two-phase Used in extraction, double-aqueous phase system includes ethanol and inorganic salt.

In addition, described inorganic salt includes one of dipotassium hydrogen phosphate, potassium dihydrogen phosphate, ammonium sulfate and sodium carbonate.

Further, in described double-aqueous phase system, the mass concentration of ethanol is 24%～30%, and the mass concentration of inorganic salt is 21%～24%.

Further, slightly raised Flammukinan crude extract using water extraction to Flammulina velutiper (Fr.) Sing to include: by weight Meter, takes 100 parts of golden needle fungus, adds 1000 parts of deionized water, boiling water bath reflux, extract, extraction time 60min, extraction time 2 Secondary, united extraction liquid, concentrate constant volume and obtain Flammukinan crude extract；

Flammukinan crude extract is carried out Flammukinan is obtained by extraction by aqueous two-phase system and include: by water extraction Obtain Flammukinan crude extract to be extracted by the double-aqueous phase system that ethanol is formed with inorganic salt, take under double-aqueous phase system Layer solution obtains Flammukinan extraction phase, and Flammukinan extraction phase is entered for 8000～14000 bag filters by molecular weight Row retention, trapped substance carries out reduced vacuum concentration and obtains final product Flammukinan.

Or, described golden needle fungus are that fresh Flammulina velutiper (Fr.) Sing dries gained, and drying temperature is 50 DEG C, and drying time is 48h, powder The golden needle fungus of broken gained cross 20 mesh sieves.

Or, described preservative includes one of sodium sorbate, methyl parahydroxybenzoate and isothiazolone, surely Determine agent and include one of Tween 80, Macrogol 4000 and Acritamer 940.

The preparation method of described Flammulina velutiper (Fr.) Sing essence, takes 0.05～0.5 part of Flammukinan, and 10 parts of deionized water is molten Solution, is a phase；Weigh 0.5～2 part of trehalose, 0.05～0.2 part of citric acid, 0.5～2 part of nicotiamide, 0.05 part of preservative, use 10 parts of deionized water dissolving, are b phase；Merging a, b phase, 0.1～0.5 part of hyaluronate sodium of addition, 0.2～0.5 part of stabilizer, from It is c phase after so dissolving；Weigh 1～10 part of glycerol, 0.5～1.5 part of 1,2-PD, 0.5～2 part of d- pantothenylol, add in c phase, Add deionized water to 100 parts, stir, obtain final product Flammulina velutiper (Fr.) Sing essence.

Advantages of the present invention is:

1st, the present invention chooses ethanol and inorganic salt and cooperatively forms extraction system, to the extraction yield of Flammukinan up to 85% ～91%, the extraction rate reached 74.3%～82.9% to Flammukinan, gained Flammukinan purity up to 63.1%～ 67.3%, the method is high and easy and simple to handle, with conventional polymer or ionic liquid and salt to the separation efficiency of Flammukinan The system being formed is compared, and greatly reduces extraction cost, overcomes the difficult recovery of target component in polymer and ionic liquid toxicity Larger shortcoming.Whole process process is simple, separation efficiency is high, and with low cost, safety non-toxic can be used for large-scale industry metaplasia Produce；

2nd, Flammukinan has moisturizing and Nutrition to skin, has antioxidation, antitumor, defying age, improves immunity Effect of power.With Flammukinan for development of raw materials facial treatment essence liquid, natural environmental-protective, safety non-toxic, nonirritant；

3rd, the Flammukinan that in the present invention, Flammulina velutiper (Fr.) Sing essence contains can improve skin elasticity with delay skin aging, There are certain radioprotective, moisture-keeping function；Glycerol, 1,2- propylene glycol, hyaluronate sodium etc. have long-acting high moisture-keeping function；D- is general Alcohol is penetrating deep into property wetting agent, and moistening effect go directly skin inner layer, fundamentally performance moistening effect；Trehalose can make skin Keep cytoactive in the adverse circumstances such as high temperature, arid, intensive ultraviolet radiation, and form one layer of protection outside horny layer Film；Nicotiamide is vitamin b₃A kind of derivant, be also cosmetic dermatology field generally acknowledge skin anti-aging composition, Fructus Citri Limoniae Acid can accelerate cutin metabolism, contribute to melanic in skin come off, the two jointly play defying age, whitening etc. effect.

Brief description

Fig. 1 is the Hydroxyl radical-scavenging of the Flammukinan that aqueous two-phase system is obtained with water extraction extraction in embodiment 1 Rate comparison diagram；

Fig. 2 is Flammulina velutiper (Fr.) Sing essence skin moisturization figure in embodiment 2；

Below in conjunction with the specific embodiment that inventor is given, technical scheme is elaborated.

Specific embodiment

A kind of preparation method of Flammukinan essence of the present invention, comprises the following steps:

Step one: by weight, take 100 parts of golden needle fungus, add 1000 parts of deionized water, boiling water bath reflux, extract, Extraction time 60min, extraction time 2 times, united extraction liquid, concentrate, constant volume, obtain Flammukinan crude extract；

Step 2: crude polysaccharides extract, polysaccharide enrichment are processed with aqueous two-phase system, obtains Flammukinan extraction phase；Double The double-aqueous phase system of aqueous phase extraction, its solvent is water, and solute is ethanol and inorganic salt.

Step 3: dialysis treatment is carried out to step 2 gained Flammukinan extract, to remove inorganic salt, by dialysis solution 55 DEG C of reduced vacuum concentrate, and concentrated solution lyophilization obtains Flammukinan.

Step 4: take 0.05～0.25 part of Flammukinan in step 3,1～10 part of glycerol, 1,2-PD 0.5～ 1.5 parts, 0.1～0.5 part of hyaluronate sodium, 0.5～2 part of trehalose, 0.05～0.2 part of citric acid, 0.5～2 part of d- pantothenylol, cigarette 0.5～2 part of amide, 0.2～0.5 part of stabilizer, 0.05 part of preservative, add deionized water to 100 parts, stir, obtain final product Flammulina velutiper (Fr.) Sing essence.

In step one, golden needle fungus are that fresh Flammulina velutiper (Fr.) Sing dries gained, and drying temperature is 50 DEG C, and drying time is 48h, pulverizes The golden needle fungus of gained cross 20 mesh sieves, sealed storage.

In step 2, the inorganic salt of double-aqueous phase system is dipotassium hydrogen phosphate, in potassium dihydrogen phosphate, ammonium sulfate, sodium carbonate One kind, in double-aqueous phase system, ethanol mass concentration is 24～30%, and Inorganic salt is 21～24%.

In step 3, bag filter molecular cut off used by dialysis treatment is 8000～14000.

In step 4, stabilizer is one of tween 80, PEG-4000 and Acritamer 940, and preservative is mountain One of pears acid sodium, methyl parahydroxybenzoate and isothiazolone.

In step 4, the compound method of essence is:

(1) take 0.05～0.25 part of the Flammukinan of purification, 10 parts of dissolvings of deionized water, are a phase；

(2) weigh 0.5～2 part of trehalose, 0.05～0.2 part of citric acid, 0.5～2 part of nicotiamide, 0.05 part of preservative, With 10 parts of deionized water dissolving, it is b phase；

(3) merge a, b phase, add 0.1～0.5 part of hyaluronate sodium, 0.2～0.5 part of stabilizer, stir, standing 24h, makes fully naturally to dissolve, is c phase；

(4) weigh 1～10 part of glycerol, 0.5～1.5 part of 1,2-PD, 0.5～2 part of d- pantothenylol, be added in c phase, mend Plus deionized water, to 100 parts, stirs, obtain final product Flammulina velutiper (Fr.) Sing essence.

The present invention provides a kind of formula of Flammulina velutiper (Fr.) Sing essence and preparation method, the safe and non-stimulating property of this formula material, protects Moistening skin, defying age, whitening effect are good, and compound method is simple and easy to operate.

In the present invention, Flammukinan used reclaims gained for acupuncture needle mushroom extracting solution after aqueous two-phase extraction.Double-aqueous phase system The aqueous solution that (aqueous two-phase systems, atps) refers to two kinds of hydrophilic compounds is mixed under certain mass concentration The two not miscible aqueous phase systems spontaneously forming after conjunction.Aqueous two-phase extraction is a kind of new liquid-liquid extraction and separation technology, behaviour Work is simple, separation efficiency is high, separation condition is gentle and technique is easily amplified, and possesses the prospect of industrial separation natural product.Pass The extraction system of system is often cooperatively formed with salt with high polymer, and high polymer cost is of a relatively high, be difficult to recycling.The present invention selects Take ethanol and inorganic salt to cooperatively form extraction system, reduce extraction cost and toxicity, base can be established for next step industrialized production Plinth.

The formula of the present invention is by Flammukinan, glycerol, 1,2-PD, hyaluronate sodium, trehalose, citric acid, d- Pantothenylol, nicotiamide, stabilizer, preservative forms.Research shows, Flammukinan can improve skin bullet with delay skin aging Property, has certain radioprotective, moisture-keeping function, has functions that antioxidation, antitumor, enhance immunity and antibacterial simultaneously, this Bright Flammulina velutiper (Fr.) Sing essence skin moisturization is tested, moisturizing rate such as Fig. 1, display Flammulina velutiper (Fr.) Sing essence moistening effect notable Glycerine-Aqueous Solution higher than 6%, also the Glycerine-Aqueous Solution compared with 6% is lasting for moisture preserving time.

Glycerol is traditional excellent wetting agent, inexpensive, but has sticky sense when using, and high concentration has certain thorn to skin Sharp property；1,2-PD have with glycerols as moisturizing intensity, but compared with glycerol milder, share the two moistening effect with glycerol All increase, the zest of glycerol can be alleviated, eliminate the sticky sense that glycerol uses；The efficient moisture-retention agent that hyaluronate sodium is well recognized as, Still play excellent moisture-keeping function in dry environment (air humidity 30% about), no any injury to human body；D- pantothenylol For the wetting agent penetrating deep into, stimulate the growth of epithelial cell, promote wound healing, play antiinflammation；Above-mentioned four kinds of wetting agents Share auxiliary Flammukinan to play more comprehensively deeper into more comfortable moistening effect.

Trehalose is referred to as " super sun-proof Moisture factor ", and its application on cosmetics is that have excellent guarantor based on it Hold cell viability and the characteristic of biomacromolecule activity, Skin Cell (especially epidermis cell) is in high temperature, drying, intensive ultraviolet Under the environment such as radiation, easily dry out generation keratinization, or even death comes off and makes skin damage.Trehalose is in this case One layer of special protecting film can be formed in cell surface layer, not only moisten Skin Cell, also external heat radiation can be gone out Going, thus protecting skin impaired, making skin health natural, flexible.

Citric acid also known as citric acid, belong to natural organic fruit acid, are mainly used in accelerating cutin renewal, the renewal of cutin helps Melanic peeling in skin, the receipts of pore are thin etc.；Nicotiamide is vitamin b₃A kind of derivant, be also beauty treatment skin The skin anti-aging composition that scientific domain is generally acknowledged；Citric acid and nicotiamide collective effect, make Flammulina velutiper (Fr.) Sing essence have and improve skin Matter, whitening and anti-aging effect.

In addition, for increasing the stability of essence, ensureing certain denseness, with the addition of the stabilizer of safety non-toxic, and peace The preservative of full high-efficiency broad spectrum.Each group divide complement each other, indispensable, jointly play moisturizing, whitening, sun-proof, defying age, improvement The effect of skin quality.Stabilizer is one of tween 80, PEG-4000 and Acritamer 940, through test, three's thermostability Tolerance to cold zero difference, compared with Macrogol 4000, Acritamer 940 consumption is less, and Tween 80 is more suitable for containing quintessence oil class The stabilizer of essence.

In order to be better understood from the present invention, to further illustrate with reference to specific embodiment.

Embodiment 1:

Fresh Flammulina velutiper (Fr.) Sing is placed in clean stainless steel pallet, dries in air dry oven to constant weight, temperature is 50 DEG C, when Between be 48h, Universalpulverizer pulverize, cross 20 mesh sieves, obtain golden needle fungus；

The extraction process of water extraction includes: takes 100 parts of golden needle fungus to be placed in round-bottomed flask, adds deionized water 1000 Part, stirring makes Flammulina velutiper (Fr.) Sing powder and deionized water be mixed thoroughly, boiling water bath reflux, extract, 2 times, and flow back 60min every time, mixes Close liquid high speed centrifuge 7000r/min and be centrifuged 20min, so that Flammulina velutiper (Fr.) Sing granulated slag is precipitated completely, take supernatant, 55 DEG C of vacuum decompressions It is concentrated into 100 parts, adds 500 parts of dehydrated alcohol, be sufficiently stirred for, 7000r/min is centrifuged 10min, remove supernatant, pellet frozen Dry Flammulina velutiper (Fr.) Sing crude polysaccharides.

The extraction process of aqueous two-phase system includes: obtains Flammulina velutiper (Fr.) Sing crude polysaccharides by above-mentioned water extraction, takes 10 parts of Flammulina velutiper (Fr.) Sing Crude polysaccharides, with 100 parts of deionized water dissolving, adding dehydrated alcohol to make its mass concentration is 28%, and adding ammonium sulfate makes it Mass concentration is 23%, fully vibrates, and static 10min, mixed liquor high speed centrifuge 7000r/min centrifugation 10min makes layering Completely, take off layer solution and obtain Flammukinan extraction phase, by extract be transferred to molecular cut off be 8000～14000 saturating In analysis bag, deionized water is dialysed 6 times, 6 hours every time.55 DEG C of reduced vacuum of dialysis solution concentrate, and concentrated solution is cryodesiccated to be arrived Flammukinan.

The purity of water extraction gained Flammulina velutiper (Fr.) Sing crude polysaccharides is 23.5%, and aqueous two-phase system gained Flammukinan purity is 67.3%.Detected for index with Scavenging action to hydroxyl free radical to extracting gained Flammukinan antioxidation, result such as Fig. 1 Shown, show that the Scavenging action to hydroxyl free radical of the Flammukinan obtained by aqueous two-phase system is significantly higher than the gold of water extraction gained Pin mushroom polysaccharide.

Embodiment 2:

Flammulina velutiper (Fr.) Sing drying meanss and Flammulina velutiper (Fr.) Sing crude polysaccharides preparation technology are with embodiment 1.

Flammulina velutiper (Fr.) Sing essence prescription is: 0.1 part of Flammukinan, 1 part of glycerol, 1 part of 1,2-PD, hyaluronate sodium 0.3 part, 1 part of trehalose, 0.1 part of citric acid, 0.5 part of d- pantothenylol, 1 part of nicotiamide, Acritamer 940 is 0.2 part, sodium sorbate 0.05 part, 94.75 parts of deionized water.

Flammulina velutiper (Fr.) Sing essence preparing process is:

Take 0.1 part of Flammukinan, with 10 parts of deionized water dissolving, be a phase；

Weigh 1 part of trehalose, 0.1 part of citric acid, 1 part of nicotiamide, 0.05 part of sodium sorbate, water-soluble with 10 parts of deionizations Solution, is b phase liquid；

Merge a, b phase, add 0.3 part of hyaluronate sodium, Acritamer 940 is 0.2 part, is sufficiently stirred for, stand 24h, make to fill Divide nature dissolving, be c phase；

Weigh 1 part of glycerol, 1 part of 1,2-PD, 0.5 part of d- pantothenylol, be added in c phase, add deionized water to 100 Part, stir, obtain final product Flammulina velutiper (Fr.) Sing essence.

The extraction yield of the Flammukinan of the present embodiment is 91%, and extraction ratio is 81.4%, and purity is 67.3%.

The moistening effect of the Flammulina velutiper (Fr.) Sing essence prepared is tested, the Glycerine-Aqueous Solution with deionized water and 6% is Comparison, measures and calculates moisturizing rate and the moisture preserving time of essence, moisturizing rate computing formula is: moisturizing rate/%=is (using elite Skin moisture content after liquid-using skin moisture content before essence)/using skin moisture content × 100 before essence, such as scheme Shown in 2, show that Flammulina velutiper (Fr.) Sing essence has good moisture retention to skin, performance of keeping humidity is significantly higher than 6% glycerol-water-soluble Liquid, and in 100min moisturizing rate still up to more than 50%.

Embodiment 3:

Flammulina velutiper (Fr.) Sing drying meanss and Flammulina velutiper (Fr.) Sing crude polysaccharides preparation technology are with embodiment 1.Except for the difference that: second in double-aqueous phase system Alcohol mass concentration is 30%, and Inorganic salt is 24%.

Flammulina velutiper (Fr.) Sing essence prescription is: 0.25 part of Flammukinan, 10 parts of glycerol, 0.5 part of 1,2-PD, hyaluronic acid 0.1 part of sodium, 2 parts of trehalose, 0.05 part of citric acid, 0.5 part of d- pantothenylol, 2 parts of nicotiamide, Acritamer 940 is 0.5 part, sorbic acid 0.05 part of sodium, 86.05 parts of deionized water.

Flammulina velutiper (Fr.) Sing essence preparing process is with embodiment 2.

The extraction yield of the Flammukinan of the present embodiment is 89%, and extraction ratio is 75.7%, and purity is 63.1%.Aqueous two-phase When in system, ethanol and ammonium sulfate mass concentration increase to 30% and 24% respectively, the extraction yield of gained Flammukinan, extraction Rate and purity all have a certain degree of reduction, illustrate that in double-aqueous phase system, the proportioning between ethanol and inorganic salt is to Flammukinan Effect of extracting have a certain impact.

Embodiment 4:

Flammulina velutiper (Fr.) Sing drying meanss and Flammulina velutiper (Fr.) Sing crude polysaccharides preparation technology are with embodiment 1.Except for the difference that: second in double-aqueous phase system The mass concentration of alcohol is 26%, and Inorganic salt is 22%.

Flammulina velutiper (Fr.) Sing essence prescription is: 0.1 part of Flammukinan, 5 parts of glycerol, 1.5 parts of 1,2-PD, hyaluronate sodium 0.3 part, 1 part of trehalose, 0.05 part of citric acid, 1 part of d- pantothenylol, 2 parts of nicotiamide, Acritamer 940 is 0.2 part, sodium sorbate 0.05 part, 88.8 parts of deionized water.

Flammulina velutiper (Fr.) Sing essence preparing process is same embodiment 2.

The extraction yield of the Flammukinan of the present embodiment is 88%, and extraction ratio is 82.9%, and purity is 65.4%.

Embodiment 5:

Flammulina velutiper (Fr.) Sing drying meanss and Flammulina velutiper (Fr.) Sing crude polysaccharides preparation technology are with embodiment 1.Except for the difference that: second in double-aqueous phase system The mass concentration of alcohol is 24%, and Inorganic salt is 21%.

Flammulina velutiper (Fr.) Sing essence prescription is: 0.05 part of Flammukinan, 1 part of glycerol, 1 part of 1,2-PD, hyaluronate sodium 0.5 part, 1 part of trehalose, 0.2 part of citric acid, 2 parts of d- pantothenylol, 1 part of nicotiamide, Acritamer 940 is 0.3 part, sodium sorbate 0.05 Part, 92.9 parts of deionized water.

Flammulina velutiper (Fr.) Sing essence preparing process is with embodiment 2.

The extraction yield of the Flammukinan of the present embodiment is 85%, and extraction ratio is 74.3%, and purity is 64.6%.

Cutaneous sense, stability, security test are carried out to embodiment 2～5.Cutaneous sense tester is 10 skins 20～30 years old personage in order；The outward appearance of Flammulina velutiper (Fr.) Sing essence, abnormal smells from the patient, cold-resistant and resistance to is detected according to qbt 2660-2004 Heat stability.

Flammulina velutiper (Fr.) Sing essence sensory evaluation standard is as shown in table 1:

Table 1 Flammulina velutiper (Fr.) Sing essence sensory evaluation standard

Sensory evaluation total score=outward appearance × 0.1+ abnormal smells from the patient × 0.1+ skin sense × 0.3+ moisture retention × 0.3+ wrinkle resistance × 0.2

Flammulina velutiper (Fr.) Sing essence Analyses Methods for Sensory Evaluation Results as shown in table 2, comment by the essence sense organ of display embodiment 2 and embodiment 4 Valency total score is higher, and in embodiment 2, glycerol, 1,2-PD, hyaluronate sodium, d- pantothenylol consumption are all less, and moisture retention is best, make Optimal with rear skin sense, it may have good wrinkle resistance.

The advantage of embodiment 4 is to the addition of more nicotiamide, has more preferable anti-wrinkle effect.

Table 2 Flammulina velutiper (Fr.) Sing essence Analyses Methods for Sensory Evaluation Results

Embodiment	Outward appearance	Abnormal smells from the patient	Skin sense	Moisture retention	Wrinkle resistance	Total score
							2	9	8	9	9	7	8.5
3	7	8	7	8	6	7.2
							4	8	9	7	9	8	8.1
5	9	6	6	8	7	7.1

Flammulina velutiper (Fr.) Sing essence stability and safety examination result are as shown in table 3: the Flammulina velutiper (Fr.) Sing elite in embodiment 2～5 Liquid is heat-resisting, tolerance to cold is all good and safe, using rear no anaphylaxiss.

Table 3 Flammulina velutiper (Fr.) Sing essence stability and safety examination result

Embodiment 6:

Inorganic salt uses dipotassium hydrogen phosphate to the present embodiment as different from Example 2.

The extraction yield of the Flammukinan of the present embodiment is 81.5%, and extraction ratio is 65.7%, and purity is 62.7%.

Embodiment 7:

Inorganic salt uses sodium carbonate to the present embodiment as different from Example 2.

The extraction yield of the Flammukinan of the present embodiment is 76.8%, and extraction ratio is 63.2%, and purity is 59.1%.

Embodiment 8:

The present embodiment is as different from Example 2:

Flammulina velutiper (Fr.) Sing essence prescription and configuration technique with embodiment 2, except for the difference that: without hyaluronate sodium.

The moistening effect of the Flammulina velutiper (Fr.) Sing essence in this embodiment is tested, result such as table 4, display embodiment 2 is protected Wet rate shares apparently higher than embodiment 8, four kinds of wetting agents, and moistening effect can significantly strengthen.

Table 4 embodiment 2 and embodiment 8 moistening effect contrast table

Claims

1. a kind of Flammulina velutiper (Fr.) Sing essence it is characterised in that include Flammukinan, glycerol, 1,2-PD, hyaluronate sodium, Trehalose, citric acid, d- pantothenylol, nicotiamide, stabilizer, preservative and water.

2. Flammulina velutiper (Fr.) Sing essence as claimed in claim 1 it is characterised in that according to the mass fraction, including Flammukinan 0.05～0.25 part, 1～10 part of glycerol, 0.5～1.5 part of 1,2-PD, 0.1～0.5 part of hyaluronate sodium, trehalose 0.5 ～2 parts, 0.05～0.2 part of citric acid, 0.5～2 part of d- pantothenylol, 0.5～2 part of nicotiamide, 0.2～0.5 part of stabilizer, preservative 0.05 part, balance of water, the total amount of mentioned component is 100 parts.

3. Flammulina velutiper (Fr.) Sing essence as claimed in claim 1 or 2 is it is characterised in that described Flammukinan is to adopt water extraction Method is slightly raised Flammukinan crude extract to Flammulina velutiper (Fr.) Sing, then by aqueous two-phase system, Flammukinan crude extract is entered Row is obtained by extraction Flammukinan；

Used in described aqueous two-phase system, double-aqueous phase system includes ethanol and inorganic salt.

4. Flammulina velutiper (Fr.) Sing essence as claimed in claim 3 is it is characterised in that described inorganic salt includes dipotassium hydrogen phosphate, phosphorus One of acid dihydride potassium, ammonium sulfate and sodium carbonate.

5. Flammulina velutiper (Fr.) Sing essence as claimed in claim 3 it is characterised in that in described double-aqueous phase system the quality of ethanol dense Spend for 24%～30%, the mass concentration of inorganic salt is 21%～24%.

6. Flammulina velutiper (Fr.) Sing essence as claimed in claim 3 is it is characterised in that slightly raised to Flammulina velutiper (Fr.) Sing using water extraction Flammukinan crude extract includes: by weight, takes 100 parts of golden needle fungus, adds 1000 parts of deionized water, boiling water bath flows back Extract, extraction time 60min, extraction time 2 times, united extraction liquid, concentrate constant volume and obtain Flammukinan crude extract；

Flammukinan crude extract is carried out Flammukinan is obtained by extraction by aqueous two-phase system and include: water extraction is obtained Flammukinan crude extract is extracted by the double-aqueous phase system that ethanol is formed with inorganic salt, takes the lower floor of double-aqueous phase system molten Liquid obtains Flammukinan extraction phase, and Flammukinan extraction phase is carried out cutting for 8000～14000 bag filters by molecular weight Stay, trapped substance carries out reduced vacuum concentration and obtains final product Flammukinan.

7. Flammulina velutiper (Fr.) Sing essence as claimed in claim 6 is it is characterised in that described golden needle fungus dry institute for fresh Flammulina velutiper (Fr.) Sing , drying temperature is 50 DEG C, and drying time is 48h, and the golden needle fungus pulverizing gained cross 20 mesh sieves.

8. Flammulina velutiper (Fr.) Sing essence as claimed in claim 1 or 2 is it is characterised in that described preservative includes sodium sorbate, right One of methyl hydroxybenzoate and isothiazolone, stabilizer is included in Tween 80, Macrogol 4000 and Acritamer 940 One kind.

9. the preparation method of the Flammulina velutiper (Fr.) Sing essence described in claim 1-8 any claim is it is characterised in that take Flammulina velutiper (Fr.) Sing 0.05～0.5 part of polysaccharide, 10 parts of dissolvings of deionized water, are a phase；Weigh 0.5～2 part of trehalose, citric acid 0.05～0.2 Part, 0.5～2 part of nicotiamide, 0.05 part of preservative, with 10 parts of deionized water dissolving, is b phase；Merge a, b phase, add hyalomitome 0.1～0.5 part of sour sodium, 0.2～0.5 part of stabilizer, is c phase after naturally dissolving；Weigh 1～10 part of glycerol, 1,2-PD 0.5 ～1.5 parts, 0.5～2 part of d- pantothenylol, add in c phase, add deionized water to 100 parts, stir, obtain final product Flammulina velutiper (Fr.) Sing elite Liquid.