CN112544616A - Method for making red algae and green algae three-dimensional specimen - Google Patents
Method for making red algae and green algae three-dimensional specimen Download PDFInfo
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- CN112544616A CN112544616A CN202011441570.6A CN202011441570A CN112544616A CN 112544616 A CN112544616 A CN 112544616A CN 202011441570 A CN202011441570 A CN 202011441570A CN 112544616 A CN112544616 A CN 112544616A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
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Abstract
The invention provides a method for preparing a red algae and green algae three-dimensional specimen, which comprises the following steps: solidifying the algae by using a magnesium chloride solution; then the algae body is put into plasticizing liquid consisting of 1/3 polyethylene glycol and 2/3 magnesium chloride solution (by volume ratio) for soaking; gradually increasing the concentration of polyethylene glycol in the plasticizing liquid for plasticizing; then putting the algae into polyethylene glycol which is toned by using the color concentrate for dyeing; taking out, fixing on stone, and sealing in glass cover. The method of the invention is simple to operate and nontoxic, can keep the original upright shape and the approximate color of the red algae or green algae specimen for a long time, improves the value of the species identification, and is beneficial to teaching and display.
Description
Technical Field
The invention relates to a method for preparing seaweed specimens, in particular to a method for preparing red algae and green algae three-dimensional specimens.
Background
The large-scale seaweed specimen used for teaching or for visiting and exhibition is mostly manufactured into a plane specimen by pressing and absorbing water. For the algae with low water content, especially for the algae in flat shape, the pressing method is adopted to change the shape of the algae slightly, and for the algae in non-flat leaf shape, especially for the algae in upright shape after dried out of the water, the pressing method greatly changes the original shape, which is not beneficial to the identification and display of the algae. Although the specimen is prepared by soaking in alcohol or formalin, although the form of seaweed can be preserved well, seaweed soaked in alcohol turns white and brittle in less than 2 months, and formalin is not so toxic that it is used for preserving specimen. Land plants can better keep the original color and shape after being subjected to color fixation and plasticizing, but before the application date, no technical scheme for preparing seaweed specimens by adopting the method is disclosed.
Disclosure of Invention
The invention aims to provide a method for preparing a red algae and green algae three-dimensional specimen, which is simple to operate and nontoxic, can keep the original upright shape and the similar color of the red algae or green algae which are still upright after being dried out of the water surface for a long time, improves the value of species identification, and is beneficial to teaching and display.
The invention is realized by the following technical scheme: a method for preparing a red algae and green algae three-dimensional specimen comprises the following steps:
a. placing red algae or green algae into magnesium chloride solution to be soaked for 1-20 days for solidification;
b. preparing a first plasticizing liquid, wherein the first plasticizing liquid comprises the following components in volume ratio: polyethylene glycol 1/3, magnesium chloride solution 2/3; soaking red algae or green algae in the first plasticizing liquid for 1 day;
c. b, mixing 1/2 volumes of the first plasticizing liquid with another polyethylene glycol according to the volume ratio of 1:1 to obtain a second plasticizing liquid, and soaking the red algae or green algae soaked in the step b in the second plasticizing liquid for 1 day;
d. c, repeating the step c for 2-3 times in a recycling manner, and mixing 1/2 volumes of the plasticizing liquid soaked with the red algae or the green algae in the previous cycle and another polyethylene glycol according to the volume ratio of 1:1 to obtain a new plasticizing liquid each time;
e. d, adding polyethylene glycol into the special chromogen solution for dripping the glue, adding the chromogen solution and the color to be approximately the original color of the red algae or the green algae, and soaking the red algae or the green algae in the step d for 3 to 30 days;
f. taking out red algae or green algae, and removing surface liquid with absorbent paper to obtain red algae or green algae specimen.
Preferably, the concentration of the magnesium chloride solution in the step a and the step b is 50-60%.
Preferably, the molecular weight of the polyethylene glycol is 200-600, and the concentration is 100%.
Further, in the step f, the red algae or green algae specimen is fixedly sealed and placed in a transparent container for storage; selecting a round, cylindrical or pear-shaped glass cover with corresponding specification according to the size and shape of a red algae or green algae specimen, wherein the base of the glass cover is a glass or rubber plug; wiping the glass cover, the base and the small stone which is treated cleanly with absolute ethyl alcohol once, and fixing the small stone in the center of the base with hot melt adhesive; fixing red algae or green algae specimen on stone with hot melt adhesive, covering with glass cover, and sealing the gap between the glass cover and the base with white wax to achieve sealing effect.
The invention has the beneficial effects that:
1. the invention adopts magnesium chloride solution to carry out solidification treatment before plasticization, fixes the three-dimensional shapes of red algae and green algae specimens, and keeps the original shapes of algae bodies. The magnesium chloride solution with higher concentration has dehydration fixed shape and antiseptic effect, while the magnesium chloride solution with more than 50 percent has better shape fixation and antiseptic effect, but the seaweed directly put into the magnesium chloride solution with the concentration higher than 60 percent has the phenomenon of shape change caused by serious dehydration, so the effect of adopting the magnesium chloride solution with 50 percent to 60 percent during solidification is most suitable.
2. According to the invention, the concentration of polyethylene glycol is gradually increased in the magnesium chloride solution to plasticize red algae and green algae, the low molecular weight (200-600) polyethylene glycol can replace water in algae to keep the form and the corrosion prevention effect, the concentration of polyethylene glycol is gradually increased to gradually carry out the plasticization process, and meanwhile, the magnesium chloride solution plays a role in solid form to avoid the form change of the algae in the plasticization process.
3. The invention can dye the plasticized green alga and red alga specimens, so that the green alga and red alga specimens can better keep the color during the preservation period. The color essence dissolved in the polyethylene glycol enters the algae along with the polyethylene glycol to replace water in the algae to replace lost algae pigment so as to enable the algae to develop color, and the pigment for the glue dripping can keep the color unchanged for a long time, so that the algae can keep better color for a long time.
4. The invention adopts the glass cover to seal, not only prevents the specimen from contacting water vapor, bacteria and mould in the air, but also is beneficial to the preservation of the specimen and the observation of the specimen; the fixing of the seaweed on the stone is based on the fact that the large seaweed mostly grows on the stone in the natural sea area, and the adoption of the mode is beneficial to displaying the original ecological mode of the large seaweed.
5. The method for preparing the red algae and green algae three-dimensional specimens is simple to operate, nontoxic and strong in practicability; the preparation method of the invention can keep the original three-dimensional shape and color of the specimen in the sea area, which greatly improves the type identification function of the specimen, thereby being beneficial to teaching and display. In addition, the preparation method of the invention can be adopted to prepare the specimen of the seaweed with ornamental value as an artwork for exhibition or as a gift, thereby opening up another economic way for the development and utilization of large-scale seaweed.
Drawings
FIG. 1A is a picture of the form of purple red round fruit cytoalgae in the sea area, FIG. 1B is a picture of the sample 1 day after preparation, and FIG. 1C is a picture of the sample 5 months after preparation;
fig. 2A is a picture of a red-brown anchovy preserved by freezing, fig. 2B is a picture of 1 day after preparation of a specimen, and fig. 2C is a picture of 5 months after preparation of a specimen;
FIG. 3A shows the form of green companion cotton alga in the sea area, FIG. 3B shows a part of algal bodies of companion cotton alga, FIG. 3C shows a picture taken 1 day after preparation of a specimen, and FIG. 3D shows a picture taken after 5 months of storage after preparation of a specimen.
Detailed Description
The present invention is further illustrated by the following specific examples.
Example 1
a. Soaking purple red Cytospora verticillata in 50% magnesium chloride solution for 1 day, and solidifying;
b. preparing a first plasticizing liquid, wherein the first plasticizing liquid comprises the following components in volume ratio: polyethylene glycol 1/3 with molecular weight of 400, 50% magnesium chloride solution 2/3; soaking Cytospora rotundifolia in the first plasticizing liquid for 1 day;
c. b, mixing 1/2 volumes of the first plasticizing liquid with another 400 molecular weight polyethylene glycol according to the volume ratio of 1:1 to obtain a second plasticizing liquid, and soaking the cycocysts orbiculatus soaked in the step b in the second plasticizing liquid for 1 day;
d. c, repeating the step c for 2-3 times in a circulating manner, and mixing 1/2 volumes of the plasticizing liquid soaked in the previous cycle with another polyethylene glycol according to the volume ratio of 1:1 to obtain a new plasticizing liquid each time;
e. adding polyethylene glycol with molecular weight of 400 into the claret solution (special color concentrate solution for dripping gelatin), stirring, adding color concentrate to adjust to approximate the original color of Cytospora rotundifolia, and soaking the Cytospora rotundifolia in the step d in the mixed solution for 3 days;
f. taking out the round fruit cyst algae, and absorbing the surface liquid by using absorbent paper to obtain a round fruit cyst algae specimen;
g. selecting a circular glass cover with corresponding specification according to the size of the round fruit cyst algae specimen, wherein the base of the glass cover is made of glass; wiping the glass cover, the base and the small stone which is treated cleanly with absolute ethyl alcohol once, and fixing the small stone in the center of the base with hot melt adhesive; fixing the round fruit cyst algae specimen on the stone with hot melt adhesive, covering a glass cover, and sealing the gap between the glass cover and the base with white wax.
The round fruit cells (see fig. 1A, the shape in the sea area) of the embodiment keep the original three-dimensional shape in the plasticizing process (see fig. 1B); the morphology and color remained good after 5 months of storage (see FIG. 1C).
Example 2
a. Soaking the refrigerated and brownish red phoenix tailed vegetable in 60% magnesium chloride solution for 3 days for solidification;
b. preparing a first plasticizing liquid, wherein the first plasticizing liquid comprises the following components in volume ratio: polyethylene glycol 1/3 with molecular weight of 200, 60% magnesium chloride solution 2/3; putting the phoenix-tail fern into the first plasticizing liquid, and soaking for 1 day;
c. b, mixing 1/2 volumes of the first plasticizing liquid with another polyethylene glycol with the molecular weight of 200 according to the volume ratio of 1:1 to obtain a second plasticizing liquid, and soaking the bracken soaked in the step b in the second plasticizing liquid for 1 day;
d. c, repeating the step c for 2-3 times in a recycling manner, and mixing 1/2 volumes of the plasticizing liquid soaked in the bracken in the previous cycle and another polyethylene glycol according to the volume ratio of 1:1 to obtain a new plasticizing liquid each time;
e. taking polyethylene glycol with the molecular weight of 400 (the concentration is 100%), dripping red and brown color concentrate solution special for dripping the glue, stirring, adding the color concentrate to be approximately the original color of the phoenix tail vegetable, and soaking the phoenix tail vegetable in the step d in the mixed solution for 20 days;
f. taking out the caulis Entadae, and removing the surface liquid with absorbent paper to obtain caulis Entadae specimen.
g. Selecting a round glass cover with corresponding specification according to the size of the phoenix-tail cabbage, wherein the base of the glass cover is made of glass; wiping the glass cover, the base and the small stone which is treated cleanly with absolute ethyl alcohol once, and fixing the small stone in the center of the base with hot melt adhesive; fixing the caulis Entadae specimen on stone with hot melt adhesive, covering with a glass cover, and sealing the gap between the glass cover and the base with white wax.
The anchovy of this example is a frozen algae body which has lost part of water (see fig. 2A), and after plasticization, the water is replaced by polyethylene glycol to restore the original form (see fig. 2B), and the form and color are better maintained after 5 months of storage (see fig. 2C).
Example 3
a. Soaking the grass green companion cotton algae in 55% magnesium chloride solution for 20 days, and solidifying;
b. preparing a first plasticizing liquid, wherein the first plasticizing liquid comprises the following components in volume ratio: polyethylene glycol 1/3 with molecular weight of 400, 55% magnesium chloride solution 2/3; soaking the companion cotton algae in the first plasticizing liquid for 1 day;
c. b, mixing 1/2 volumes of the first plasticized solution with another 400 molecular weight polyethylene glycol according to the volume ratio of 1:1 to obtain a second plasticized solution, and soaking the gracilaria cordycepin soaked in the step b in the second plasticized solution for 1 day;
d. c, repeating the step c for 2-3 times in a recycling manner, and mixing 1/2 volumes of the plasticizing liquid soaked with the cotton-wool algae in the previous cycle and another polyethylene glycol with the molecular weight of 400 according to the volume ratio of 1:1 to obtain new plasticizing liquid each time;
e. adding polyethylene glycol with molecular weight of 600 (concentration of 100%) into the solution of the special grass green essence for dripping the gelatin, stirring, adding the essence to the solution to adjust the color to be close to the original color of the Paramiaquilensis, and soaking the Paramiaquilensis in the step d in the mixed solution for 10 days;
f. taking out the companion cotton algae, and absorbing the surface liquid by using absorbent paper to obtain the companion cotton algae specimen.
g. Selecting a circular glass cover with corresponding specification according to the size of the sponge algae specimen, wherein the base of the glass cover is made of glass; wiping the glass cover, the base and the small stone which is treated cleanly with absolute ethyl alcohol once, and fixing the small stone in the center of the base with hot melt adhesive; fixing the sponge algae specimen on the stone with hot melt adhesive, covering with a glass cover, and sealing the gap between the glass cover and the base with white wax.
The companion cotton algae of this example (see fig. 3A and 3B) maintains its original stereo morphology during the plasticizing process (see fig. 3C); morphology and color remained good after 5 months of storage (see figure 3D).
The present invention may be embodied in other specific forms without departing from its spirit or essential characteristics. The above-described embodiments of the present invention are to be considered in all respects as illustrative and not restrictive. Therefore, any minor modifications, equivalent changes and modifications to the above embodiments according to the spirit of the present invention are within the scope of the technical solution of the present invention.
Claims (4)
1. A method for preparing a red algae and green algae three-dimensional specimen is characterized by comprising the following steps: the method comprises the following steps:
a. placing red algae or green algae into magnesium chloride solution to be soaked for 1-20 days for solidification;
b. preparing a first plasticizing liquid, wherein the first plasticizing liquid comprises the following components in volume ratio: polyethylene glycol 1/3, magnesium chloride solution 2/3; soaking red algae or green algae in the first plasticizing liquid for 1 day;
c. b, mixing 1/2 volumes of the first plasticizing liquid with another polyethylene glycol according to the volume ratio of 1:1 to obtain a second plasticizing liquid, and soaking the red algae or green algae soaked in the step b in the second plasticizing liquid for 1 day;
d. c, repeating the step c for 2-3 times in a recycling manner, and mixing 1/2 volumes of the plasticizing liquid soaked with the red algae or the green algae in the previous cycle and another polyethylene glycol according to the volume ratio of 1:1 to obtain a new plasticizing liquid each time;
e. d, adding polyethylene glycol into the special chromogen solution for dripping the glue, adding the chromogen solution and the color to be approximately the original color of the red algae or the green algae, and soaking the red algae or the green algae in the step d for 3 to 30 days;
f. taking out red algae or green algae, and removing surface liquid with absorbent paper to obtain red algae or green algae specimen.
2. The method for preparing a three-dimensional specimen of red algae and green algae according to claim 1, comprising: the concentration of the magnesium chloride solution in the step a and the step b is 50-60%.
3. The method for preparing a three-dimensional specimen of red algae and green algae according to claim 1, comprising: the molecular weight of the polyethylene glycol is 200-600.
4. The method for preparing a three-dimensional specimen of red algae and green algae according to claim 1, comprising: f, the red algae or green algae specimen is fixedly sealed and placed in a transparent container for storage; the method comprises the following specific steps: selecting a round, cylindrical or pear-shaped glass cover with corresponding specification according to the size and shape of the red algae or green algae specimen, wherein the base of the glass cover is a glass or rubber plug; wiping the glass cover, the base and the small stone which is treated cleanly with absolute ethyl alcohol once, and fixing the small stone in the center of the base with hot melt adhesive; fixing red algae or green algae specimen on stone with hot melt adhesive, covering with glass cover, and sealing the gap between the glass cover and the base with white wax.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011441570.6A CN112544616A (en) | 2020-12-11 | 2020-12-11 | Method for making red algae and green algae three-dimensional specimen |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011441570.6A CN112544616A (en) | 2020-12-11 | 2020-12-11 | Method for making red algae and green algae three-dimensional specimen |
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Citations (5)
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|---|---|---|---|---|
| US4278715A (en) * | 1978-08-18 | 1981-07-14 | Queen's University At Kingston | Preservation of green plant tissues |
| JP2003215098A (en) * | 2002-01-22 | 2003-07-30 | Jokoh Co Ltd | Micro protein electrophoretic apparatus due to silver staining techniques |
| CN101401567A (en) * | 2008-03-19 | 2009-04-08 | 中国科学院新疆生态与地理研究所 | Eremophyte steeping specimen and method for making protective color |
| CN103548817A (en) * | 2013-11-18 | 2014-02-05 | 重庆市中药研究院 | Preparation method of plant plasticized specimen |
| CN107047545A (en) * | 2017-03-21 | 2017-08-18 | 广东药科大学 | A kind of method of green plants plasticizing sample and its application of obtained sample |
-
2020
- 2020-12-11 CN CN202011441570.6A patent/CN112544616A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4278715A (en) * | 1978-08-18 | 1981-07-14 | Queen's University At Kingston | Preservation of green plant tissues |
| JP2003215098A (en) * | 2002-01-22 | 2003-07-30 | Jokoh Co Ltd | Micro protein electrophoretic apparatus due to silver staining techniques |
| CN101401567A (en) * | 2008-03-19 | 2009-04-08 | 中国科学院新疆生态与地理研究所 | Eremophyte steeping specimen and method for making protective color |
| CN103548817A (en) * | 2013-11-18 | 2014-02-05 | 重庆市中药研究院 | Preparation method of plant plasticized specimen |
| CN107047545A (en) * | 2017-03-21 | 2017-08-18 | 广东药科大学 | A kind of method of green plants plasticizing sample and its application of obtained sample |
Non-Patent Citations (3)
| Title |
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| 唐安科: "绿色植物标本的塑化方法", 《生物学教学》 * |
| 张有义: ""聚乙二醇塑化制作藓类植物生态状态标本新方法"", 《生物学通报》 * |
| 殷占堂: ""一种新型的植物标本保存法"", 《植物杂志》 * |
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Application publication date: 20210326 |
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