CN208783649U - A kind of mold for Sargassum horneri seedling preparation of specimen - Google Patents
A kind of mold for Sargassum horneri seedling preparation of specimen Download PDFInfo
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- CN208783649U CN208783649U CN201821288682.0U CN201821288682U CN208783649U CN 208783649 U CN208783649 U CN 208783649U CN 201821288682 U CN201821288682 U CN 201821288682U CN 208783649 U CN208783649 U CN 208783649U
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- seedling
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- sargassum horneri
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- 241001260874 Sargassum horneri Species 0.000 title claims abstract description 74
- 238000002360 preparation method Methods 0.000 title claims abstract description 39
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000000741 silica gel Substances 0.000 claims abstract description 14
- 229910002027 silica gel Inorganic materials 0.000 claims abstract description 14
- 239000011521 glass Substances 0.000 claims description 27
- 239000005357 flat glass Substances 0.000 claims description 16
- 239000004033 plastic Substances 0.000 claims description 12
- 230000037431 insertion Effects 0.000 claims 1
- 238000003780 insertion Methods 0.000 claims 1
- 239000003292 glue Substances 0.000 abstract description 53
- 239000000084 colloidal system Substances 0.000 abstract description 7
- 230000000149 penetrating effect Effects 0.000 abstract description 5
- 239000000123 paper Substances 0.000 description 14
- 238000004519 manufacturing process Methods 0.000 description 13
- 238000000034 method Methods 0.000 description 13
- 241001474374 Blennius Species 0.000 description 10
- 241000195493 Cryptophyta Species 0.000 description 10
- 230000000052 comparative effect Effects 0.000 description 10
- IISBACLAFKSPIT-UHFFFAOYSA-N bisphenol A Chemical compound C=1C=C(O)C=CC=1C(C)(C)C1=CC=C(O)C=C1 IISBACLAFKSPIT-UHFFFAOYSA-N 0.000 description 8
- 230000008014 freezing Effects 0.000 description 8
- 238000007710 freezing Methods 0.000 description 8
- 238000012545 processing Methods 0.000 description 8
- 238000000926 separation method Methods 0.000 description 7
- 238000001035 drying Methods 0.000 description 6
- 238000002156 mixing Methods 0.000 description 6
- 238000007711 solidification Methods 0.000 description 6
- 230000008023 solidification Effects 0.000 description 6
- IJVRPNIWWODHHA-UHFFFAOYSA-N 2-cyanoprop-2-enoic acid Chemical compound OC(=O)C(=C)C#N IJVRPNIWWODHHA-UHFFFAOYSA-N 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 238000004321 preservation Methods 0.000 description 5
- 230000018044 dehydration Effects 0.000 description 4
- 238000006297 dehydration reaction Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 239000003822 epoxy resin Substances 0.000 description 4
- 229920000647 polyepoxide Polymers 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 241000195474 Sargassum Species 0.000 description 3
- 238000005520 cutting process Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000000877 morphologic effect Effects 0.000 description 3
- 230000003252 repetitive effect Effects 0.000 description 3
- 238000005464 sample preparation method Methods 0.000 description 3
- 238000004513 sizing Methods 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 2
- 238000004026 adhesive bonding Methods 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 239000010410 layer Substances 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 238000011017 operating method Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000003892 spreading Methods 0.000 description 2
- 230000007480 spreading Effects 0.000 description 2
- 238000009966 trimming Methods 0.000 description 2
- 238000005491 wire drawing Methods 0.000 description 2
- 241000512259 Ascophyllum nodosum Species 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 208000029154 Narrow face Diseases 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 239000002390 adhesive tape Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- XYIBRDXRRQCHLP-UHFFFAOYSA-N ethyl acetoacetate Chemical compound CCOC(=O)CC(C)=O XYIBRDXRRQCHLP-UHFFFAOYSA-N 0.000 description 1
- 125000004494 ethyl ester group Chemical group 0.000 description 1
- 210000000887 face Anatomy 0.000 description 1
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- 239000011888 foil Substances 0.000 description 1
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- 238000007654 immersion Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
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- 230000035699 permeability Effects 0.000 description 1
- 229920000570 polyether Polymers 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- QQONPFPTGQHPMA-UHFFFAOYSA-N propylene Natural products CC=C QQONPFPTGQHPMA-UHFFFAOYSA-N 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
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- 150000003839 salts Chemical class 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
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Landscapes
- Cultivation Of Seaweed (AREA)
Abstract
The utility model discloses a kind of molds for Sargassum horneri seedling preparation of specimen, it includes support frame and the die main body made of silica gel, by hollow rectangular tube and, the mold holder of tetrapyamid shape forms support frame as described above, the mold holder is inserted into rectangular tube, and the top surrounding of the mold holder and the top of rectangular tube are connected to one；The die main body, which is bottom surface opened and side, splices the tetrapyamid shape structure surrounded by 4 triangle silica gel pieces, the open-top of the mold holder and for placing inverted die main body.The Sargassum horneri seedling sample of the utility model preparation not only obtains more angles and viewing surface under less glue dosage under naked eyes state observation, moreover, non-specimen location colloid is penetrating, without bubble, Sargassum horneri seedling state stretches naturally, lines is clear.
Description
Technical field
The utility model belongs to seaweed preparation of specimen technical field, and in particular to a kind of for Sargassum horneri seedling preparation of specimen
Mold.
Background technique
Sargassum horneri (Sargassum horneri) algae plant height is big, with luxuriant foliage and spreading branches in leafy profusion, is Warm Temperate Zone In China sea area neritic area sea jungle
Main kelp species in flakes.Its frond is yellowish-brown, tree-shaped, with luxuriant foliage and spreading branches in leafy profusion, 0.5-1 meters high, up to 7 meters.Major branch circle
Cylindricality, lower part have several to indulge away shallow ridges, and 1.5~3 millimeters of diameter.Alternate, to branch estranged, chant piece lanceolar, middle rib and top, sawtooth
Drastic crack.Handle is elongated.Air bag is cylindrical, and 0.5~l centimetres long, 2~3 millimeters of diameter, both ends are tapering, hat cry of certain animals plumage split, middle rib and top,
Holdfast rip-panel shape.Receptacle is cylindrical, there is short handle, and antheridiophore is 4~8 centimetres, 1.5~2 millimeters of diameter long；It is female to protract 1.5~3
Centimetre, 2~3 millimeters of diameter.The biggish dry damp line of stormy waves is grown in give birth on the rock down toward 3 meters at or low tide band Shi Zhaozhong
Long 3~May of peak period.In order to carry out biology morphology observation to Sargassum horneri, to fully understand its different lines feature and history of life hair
It educates the stage, and carries out other biological analysis, generally require to prepare Sargassum horneri sample.
The method of the production sample of algae mainly passes through dehydration and drying at this stage, fixed, then carries out press mold encapsulation.Invention
104616578 A of patent CN discloses a kind of manufacture craft of sargassum fusifome sample, includes the following steps；Select dish；Removal of impurities takes off
Salt；Desugar；Cleaning；Dehydration；Zhan Zhi, trimming；Form is fixed；Flatten water suction；It dries in the shade setting；Gluing, fixation；Sizing；Label；Dress
Mounting；The preparation of specimen to sargassum fusifome different lines feature and the stage of development history of life is completed, is dwelt by the sheep for the method preparation invented
Dish sample is able to maintain its script growthform and color, can long-term preservation, be conducive to scientific research and practical teaching.Invention is special
Sharp 101548670 A of CN discloses a kind of production method of seaweed sample, successively includes sample cleaning, sample immersion, sample paper
Set lining and sample drying steps, sample soaking step is to be immersed in sample in soak according to nature, sample paper set lining step
It suddenly is to serve as a contrast sample on sample paper according to the nature of sample, by sample after being held out in soak in sample drying steps
One of before in step, the plastic foil in cover lining on sample face, sample drying steps are that 6~10 layers of water suction paper washer exist
Below sample paper, make sample dry with specimen holder clamping, it is every 2 after 3 days that it is primary that preceding drying in 3 days need to replace blotting paper daily
Replacement blotting paper is primary within~5 days；The invention is that a kind of sample breakage is less, sample surface gloss is preferable, sample and sample paper
The stronger seaweed sample of bonding production method, this method also reduces the labor intensity of production sample.
The seaweed sample prepared by existing method is relatively flat, is readily transported, can save the long period.But there is also with
Lower disadvantage: (1) originally three-dimensional seaweed is pressed and carries out observation preservation for plane by existing preparation of specimen's technology, it is thus impossible to see
Observe the stereochemical structure of algae；(2) simultaneously, because fabrication cycle is longer, frond is longer with air contact time, and there are colors for frond
Frond has larger difference with form in characterization when oxidation stain situation, sample and fresh picking；(3) and it is existing can only be to frond
It carries out the production of sample and step is complicated, therefore most of is all that acquisition is made at algae.It can be seen that currently, algae
Preparation of specimen be all using at algae as sample, still, since seaweed seedling is small in size, the delicate and factors such as not easy to operate, no
The operating methods such as tabletting, drying are adapted for, therefore, present technology carries out seaweed preparation of specimen by sample of seedling not yet
Precedent.But the history of life of seaweed (including Sargassum horneri) includes the developmental process of seedling from small to large, therefore, acquires Sargassum horneri seedling
And preparation of specimen is carried out to it and is of great significance to the research of its history of life etc..
Summary of the invention
In view of the drawbacks of the prior art and insufficient, the utility model is a kind of for Sargassum horneri seedling preparation of specimen by providing
Mold and its application method, to solve not using due to the factors such as Sargassum horneri seedling is small in size, delicate, not easy to operate in the prior art
In the preparation of specimen the technical issues of, to save the Sargassum horneri seedling of cultivation is completely three-dimensional.
To achieve the above object, the technical solution of the utility model are as follows:
A kind of mold for Sargassum horneri seedling preparation of specimen comprising support frame and the die main body made of silica gel, institute
Stating support frame, by hollow rectangular tube and the mold holder of tetrapyamid shape forms, and the mold holder is inserted into rectangular tube
Interior, the top surrounding of the mold holder and the top of rectangular tube are connected to one；The die main body is opened for bottom surface
It puts and the tetrapyamid shape structure surrounded, the open-top and use of the mold holder by 4 triangle silica gel pieces is spliced in side
In the inverted die main body of placement.
Preferably, the rectangular tube and mold holder are made of PVC plastic.
Preferably, the mold further includes the fixed device of a sample, and the fixed device of the sample is transparent sheet glass/glass
Glass plate.
Preferably, a length of 15cm in the bottom edge of the die main body, a length of 20cm of rib are highly 17cm.
Preferably, the side of the die main body is equipped with containing mark.
Preferably, the size of the sheet glass/glass plate is 10cm × 3cm.
Further, the utility model additionally provides the application method of the mold for Sargassum horneri seedling preparation of specimen,
Comprising the following specific steps
(1) it the processing of sample: chooses target Sargassum horneri seedling and is cut off together together with seedling rope；
(2) fixation of Sargassum horneri seedling: α-cyanoacrylate is added dropwise in the center of the fixed device of sample, seedling rope is sticked at
On the fixed device of sample, after >=5min, the fixed device of sample is stained with the one of Sargassum horneri seedling and is placed face down on inside die main body,
And keep the fixed device of sample horizontal positioned；
(3) injecting glue: glue is poured slowly into die main body；
(4) separation of sample: die main body is placed in after room temperature 10h to ensure that glue solidifies, by die main body from mould
It is taken out in tool holder, the contact surface of freezing plastic and silica gel piece is gently rubbed from die main body outside, so that freezing plastic and mold
Main body separation, finally completely takes out freezing plastic from die main body, i.e. acquisition Sargassum horneri seedling sample.
Preferably, in step (1), the length of Sargassum horneri seedling is 1cm, and the length for cutting rear seedling rope is 10cm.
Preferably, in step (3), the glue is mixed glue solution, and the mixed glue solution is by bisphenol A epoxide resin and polyethers
Amine D-230 is mixed according to the volume ratio of 1:2.5, mixing total amount≤50ml of each mixed glue solution；The mixed glue solution
The preparation method comprises the following steps: the bisphenol A epoxide resin got ready and polyetheramine D-230 are poured into container, stirred with glass bar to not drawing
Silk, is placed in 40 DEG C of water-baths after no bubble for glue, is placed in room temperature, that is, obtains the mixed glue solution.
Further, in step (3), the mixed glue solution is poured slowly into die main body, simultaneously timing is stood, during which uses again
Identical mixed glue solution preparation method prepares 50mL mixed glue solution, will be new after the mixed glue solution solidification 20min that the last time pours into
The mixed glue solution of preparation pours into mold, until the amount of repetitive operation to mixed glue solution there be not the fixed device of sample just.
Compared with prior art, the utility model has the advantages that
(1) the utility model uses pyramid shape mold for the first time, for common square mould, is guaranteeing
360 ° of field of view is provided for Sargassum horneri seedling sample while glue dosage is greatly saved.
(2) mould structure of the utility model is simple, cheap, and application method is simple and may be reused.
(3) the utility model carries out the production of sample using Sargassum horneri for the first time, and for the first time using Sargassum horneri seedling rather than at algae
Algae is subjected to preparation of specimen's preservation with three-dimensional state as sample, and for the first time.
(4) compared to traditional envelope pressure type Slide processing, the utility model uses the preserving type of stereoscopic-state, energy
Sargassum horneri growth conditions are preferably saved, while reducing destruction of the conventional method to Seedling Color and form.
(5) moreover, compared to traditional envelope pressure type Slide processing, the seaweed sample of the utility model production is more firm
Gu it is more easy to maintain, it is not easily susceptible to destroy, and observed through penetrating glue, it is more attractive.
(6) the utility model saves seedling together with seedling rope, preferably characterize seedling grown on seedling rope it is true
Real state, and solve the technical issues of seedling preservation.
(7) the Slide processing operating procedure of the utility model is simple, easily grasps, the period is shorter, and labor is greatly saved
Power.
In conclusion the utility model preparation Sargassum horneri seedling sample under naked eyes state observation, not only in less glue
More angles and viewing surface are obtained under dosage, moreover, non-specimen location colloid is penetrating, without bubble, Sargassum horneri seedling state is stretched
Exhibition is naturally, lines is clear.
Detailed description of the invention
The present invention will be further described with reference to the accompanying drawings and examples.
Fig. 1 is the structural schematic diagram of the support frame of the utility model；
Fig. 2 is the enlarged structure schematic diagram of the die main body part of the utility model；
Fig. 3 is Sargassum horneri seedling schematic diagram fixed on glass plate/sheet glass；
Fig. 4 is the schematic diagram for placing the glass plate/sheet glass for securing Sargassum horneri seedling in die main body；
Appended drawing reference: 1, die main body；1-1, silica gel piece；2, rectangular tube；3, Miao Sheng；4, the fixed device of sample；5, Sargassum horneri
Seedling；6, mold holder.
Specific embodiment
The utility model is described in further detail With reference to embodiment.
Embodiment 1
As shown in Figs 1-4, a kind of mold for Sargassum horneri seedling preparation of specimen comprising by PVC plastic (other can
To supporting role hard material also can) made of support frame and die main body 1 made of silica gel, support frame as described above is by hollow
Rectangular tube 2 and the mold holder 6 of tetrapyamid shape forms, the mold holder 6 is inserted into rectangular tube 2, the mould
The top of the 6 top surroundings and rectangular tube 2 that have holder is connected to one；The die main body 1 is bottom surface opened and side
Face surrounds tetrapyamid shape structure by 4 triangle silica gel piece 1-1 splicing, the open-top of the mold holder 6 and for putting
Set inverted die main body 1.The a length of 15cm in bottom edge of the die main body 1, a length of 20cm of rib are highly 17cm, the mold
The side of main body 1 is equipped with containing mark (not shown), can refer to the volume of sample, carries out the configuration of adhesive liquid quantity.
The production method of the Sargassum horneri seedling sample, comprising the following specific steps
(1) it the processing of sample: chooses Sargassum horneri seedling 5 and is cut off together together with seedling rope 3.The length of Sargassum horneri seedling is 1cm or so
It is advisable, the length for cutting rear seedling rope is 10cm or so.
Because Sargassum horneri seedling takes root in seedling rope, the utility model pass through the study found that seedling rope is made into sample together can be more
Sargassum horneri is characterized well in the growth conditions of Miao Shengshang, if seedling to be removed to the production for carrying out sample from Miao Shengshang, seedling is compared
It is delicate and engaged closely with seedling rope, it is not readily separated, is easily destroyed the overall structure of Sargassum horneri seedling.
(2) fixation of Sargassum horneri seedling: select one piece of 10cm × 3cm transparent glass sheet/glass plate as the fixed device of sample
4, α-cyanoacrylate is dripped in sheet glass/glass plate center, seedling rope is adhered thereto.To alpha-cyano propylene after 5min
After acetoacetic ester solidification, the fixed device of sample is stained with the one of Sargassum horneri seedling and is placed face down on inside die main body, and make sheet glass/
Glass plate is horizontal positioned.
α-cyanoacrylate is the main component of 502 glue, and setting time is short, mainly by its cementation seedling rope to consolidate
Determine Sargassum horneri seedling.Sheet glass/the glass plate is expendable consumed product, sheet glass/glass plate small and light in view of Sargassum horneri seedling
For lightweight sample is bonded, to increase the overall weight of sample, to avoid the sample of the lightweight after injecting glue floating
It rises, and because of its transparent morphological observation for not influencing sample.If sample is heavier, then do not need using sheet glass/glass plate.
(3) injecting glue: by bisphenol A epoxide resin (Ou Sibang new material Co., Ltd, Shenzhen) and (Shenzhen polyetheramine D-230
Ou Sibang new material Co., Ltd, city) according to the volume ratio (or according to 1:3 weight ratio) of 1:2.5 mixing in container is poured into, it uses
Glass bar is stirred to no wire drawing, and glue is placed in 40 DEG C of water-baths after no bubble, room temperature is placed in, that is, obtains the mixing
Glue.The mixed glue solution is poured slowly into die main body, simultaneously timing is stood, is during which prepared again with identical mixed glue solution
Method prepares 50mL mixed glue solution, and after the mixed glue solution solidification 20min that the last time pours into, the mixed glue solution newly prepared is poured into
In mold, until the amount of repetitive operation to mixed glue solution there be not sheet glass/glass plate just.
The preparation amount of each mixed glue solution must not exceed 50ml.Because colloid composition excessively mixing meeting heat production, leads to colloid
Bubble is generated, the permeability of finished product is influenced, therefore when preparing mixed glue solution, each mixing amount is using 50mL as the upper limit.
(4) separation of sample: die main body is placed in after room temperature 10h to ensure that glue solidifies, by die main body from mould
It is taken out in tool holder, the contact surface of freezing plastic and silica gel piece is gently rubbed from die main body outside, so that freezing plastic and mold
Main body separation, finally completely takes out freezing plastic from die main body, i.e. acquisition Sargassum horneri seedling sample.The utility model after taking-up
Mold it is also reusable.It can be seen that die main body is prepared using silica gel, the sample and mold after being conducive to solidification
Separation easily causes sample to knock against hard, cracks or even be crushed relative to existing manual rolling shake, and silica gel mould can not only be rapid
Mold and sample are subjected to soft separation, are more advantageous to the integrality for guaranteeing sample.
The mold and Slide processing of the present embodiment, can be not only used for the preparation of specimen of Sargassum horneri seedling, can also
Preparation of specimen for seaweed seedling similar in other.
The Sargassum horneri seedling sample prepared for embodiment 1 carries out observation evaluation: the sample made by embodiment 1 is in naked eyes shape
Under state, non-specimen location colloid is penetrating, and without bubble, the Sargassum horneri seedling state inside sample stretches naturally, lines is clear；And children
Seedling frond is complete, without breakage；Stereochemical structure (whole structural form and range of extension when Sargassum horneri seedling state and new picking
Deng) there is no difference.It therefore, can connection by the sample that embodiment 1 makes to Sargassum horneri growth of seedling state and its with seedling rope
State is observed.Also, Sargassum horneri seedling sample prepared by embodiment 1 is tetrapyamid shape, can be in smaller glue dosage (no large space
Glue waste) under conditions of obtain 360 ° of preferable observing effects, and the observing effect in 5 faces of rectangular pyramid is all fine.Moreover,
The method of embodiment 1 is easy to operate, and the period is short, and interior for 24 hours to complete, the practical laboratory operating time (removes and waits most
Gelling is solid eventually) 4h or so.
Comparative example 1 makes Sargassum horneri seedling sample at algae preparation of specimen technology using existing
Unlike the first embodiment, the rectangular-shape mold (hard, non-silica gel) of open-top is selected to prepare rectangular mark
This.Its mould structure is different from embodiment 1, but the preparation method of sample is same as Example 1.
The production method of the Sargassum horneri seedling sample, comprising the following specific steps
(1) it the processing of sample: chooses Sargassum horneri seedling and is cut off together together with seedling rope.The length of Sargassum horneri seedling is that 1cm or so is
Preferably, the length for cutting rear seedling rope is 10cm or so.
(2) fixation of Sargassum horneri seedling: selecting one piece of 10cm × 3cm transparent glass sheet/glass plate to fix device as sample,
α-cyanoacrylate is dripped in sheet glass/glass plate center, seedling rope is adhered thereto.To alpha-cyanoacrylate after 5min
After ethyl ester solidification, the fixed device of sample is stained with the one of Sargassum horneri seedling the mould inside for being placed face down on rectangular-shape, and make glass
Glass piece/glass plate is horizontal positioned.
(3) injecting glue: by bisphenol A epoxide resin and polyetheramine D-230 according to the volume ratio of 1:2.5 (or according to 1:3 weight
Than) mixing in container is poured into, it is stirred with glass bar to no wire drawing, glue is placed in 40 DEG C of water-baths after no bubble, is set
In room temperature, that is, obtain the mixed glue solution.The mixed glue solution is poured slowly into the mold of rectangular-shape, stand and is counted
When, 50mL mixed glue solution during which is prepared with identical mixed glue solution preparation method again, the mixed glue solution solidification poured into the last time
After 20min, the mixed glue solution newly prepared is poured into the mold of rectangular-shape, the amount of repetitive operation to mixed glue solution there was not glass just
Until glass piece/glass plate.
(4) separation of sample: the die main body of rectangular-shape is placed in after room temperature 10h to ensure that glue solidifies, will be grown
The mold of cube shape is buckled to or is shaken so that freezing plastic is separated with die main body, finally completely takes freezing plastic from die main body
Out, that is, Sargassum horneri seedling sample is obtained.
Existing mold is mostly cubic, embodiment 2 using the utility model embodiment 1 sample preparation method and tie
It closes existing mold and prepares Sargassum horneri seedling sample.
Sargassum horneri seedling sample prepared by comparative example 1 to be observed under naked eyes state, non-specimen location colloid is penetrating,
There is no bubble, Sargassum horneri seedling state stretches naturally, lines is clear.It can be seen that preparation of specimen of the comparative example 1 using embodiment 1
Method is capable of the original form of maximum protection Sargassum horneri seedling.But, hence it is evident that as can be seen that comparative example 1 uses normal glue consumption
Selection cuboid (contain cube) shape Mold Making at rectangular-shape sample, be only capable of in former and later two larger surfaces
Long-pending viewing surface could preferably observe Sargassum horneri seedling morphology, but in the side of its left and right two, top surface and these areas of bottom surface
More narrow face can not sufficiently observe frond form.For the mold of rectangular shape, if it is desired to obtaining greater angle
Good observing effect must just improve the height (assuming that it is length and width that positive two sides of sample, which represent) of sample, this
The dosage of glue will be greatly increased.Therefore, same glue dosage, compared to comparative example 1, the mold of the tetrapyamid shape of embodiment 1
360 ° of preferable observing effects can be more obtained under conditions of smaller glue dosage.
Comparative example 2
The Sargassum horneri seedling for choosing the marine growth of seedling length 1cm or so is cut off together about 10cm length together with seedling rope, and 4
~8 DEG C of deepfreezes are protected from light and are transported to laboratory；After being placed in laboratory, seawater is placed in 24 DEG C through the slow recovery temperature of 3~5h
In, unfold sample sufficiently.The magazines such as macroscopic other periphtic algaes and animal are removed by hand, slowly rinse 3 with fresh water
~5min sloughs sample surface salinity；Sample is set in distilled water again and is cleaned multiple times, until it is brown to thoroughly remove its remained on surface
Phycocolloid and salinity；Seedling is carefully separated from Miao Shengshang, sample is flattened at plate one by one, is gently inhaled with blotting paper
Take its excess surface moisture；
Sample is set blotting paper that is fixed, paving or newspaper surface, is filled seedling with dissecting needle and tweezers by Zhan Zhi, trimming
Point expansion, flattens water suction, according to sample size after fixation, selects the specimen holder of size appropriateness, and by sample set it is prior complete it is more
On layer blotting paper, multilayer newspaper is placed on top again, this step can carry out multiple-layer stacked；It dries in the shade setting, compresses, fixed preparation
Folder, sets room temperature (room temperature at this place refers to conventional chambers temperature), is protected from light, ventilation, the blotting paper of replacement in every 3~4 days, until mark
This sufficiently dehydration sizing, meanwhile, sample can be in this stepping row long-term preservation.Sample after being formed is taken out, adhesive tape is removed, by sample
This one side uniformly, smears latex in right amount, under the premise of holding sufficiently protrudes each portion's morphological feature, gradually pastes and set prior preparation
On good sample cardboard；Sample of the gluing after fixed to be set in the specimen holder for laying blotting paper in advance, sizing fixes 3~5 days, until
It after set time, is done to glue and sample is fixed on specimen holder, taken out fixed sample, fixing situation is gone through, if dredging
Leak adhesive consolidates organ, can be fixed with dissecting needle from new glue；Appropriately sized label paper is selected, when marking sample title, acquisition in detail
Between, information, the mounting such as collecting location, Life Stages, main morphological features and life habit select the appropriate sample mounting of size
The specimen plate for marking sargassum fusifome sample is packed into wherein by frame, and splash guard, rear sealing sample are added in back.
The observation evaluation result of sample prepared by comparative example 2:
(1) Sargassum horneri seedling morphology is smooth, can be clearly apparent the lines of blade, but blade and growthform when just adopting are inconsistent
(since long-time dehydration is formed), it is difficult to the practical growth conditions of seedling are observed by this sample.
(2) the less root that can see seedling in the Sargassum horneri seedling sample of comparative example 2, sample is mostly the children of fracture of root
Seedling, because the Sargassum horneri seedling of 1cm is more delicate, root is tightly planted in seedling rope, and method cannot save together seedling rope, is needed copper
Algae seedling removes from Miao Shengshang, in operating process, then can destroy Sargassum horneri rhizoid.
(3) the Slide processing operation of comparative example 2 is complicated, and the period is long.
Therefore, compared to for the sample preparation method of the mold of comparative example 1 and documents 2, the utility model
For the mold and sample preparation method of Sargassum horneri seedling preparation of specimen, can not only be obtained more under conditions of smaller glue dosage
Multiple preferable observing effects in face, and by the sample of the utility model preparation under naked eyes state, non-specimen location colloid is logical
Thoroughly, without bubble, Sargassum horneri seedling state stretches naturally, lines is clear.
The foregoing is merely the preferred embodiments of utility model, are not intended to limit the utility model, all in this reality
Within novel spirit and principle, any modification, equivalent replacement, improvement and so on should be included in the utility model
Within protection scope.
Claims (6)
1. a kind of mold for Sargassum horneri seedling preparation of specimen, it is characterised in that: including support frame and the mold made of silica gel
Main body, by hollow rectangular tube and, the mold holder of tetrapyamid shape forms support frame as described above, the mold holder insertion
To rectangular tube, the top surrounding of the mold holder and the top of rectangular tube are connected to one；The die main body
For bottom surface opened and the tetrapyamid shape structure surrounded, the top of the mold holder are spliced by 4 triangle silica gel pieces in side
It opens and for placing inverted die main body.
2. the mold according to claim 1 for Sargassum horneri seedling preparation of specimen, it is characterised in that: the rectangular tube and mould
Tool holder is made of PVC plastic.
3. the mold according to claim 1 for Sargassum horneri seedling preparation of specimen, it is characterised in that: the die main body
The a length of 15cm in bottom edge, a length of 20cm of rib are highly 17cm.
4. the mold according to claim 1 for Sargassum horneri seedling preparation of specimen, it is characterised in that: the mold further includes
One sample fixes device, and the fixed device of the sample is transparent sheet glass/glass plate.
5. the mold according to claim 4 for Sargassum horneri seedling preparation of specimen, it is characterised in that: the sheet glass/glass
The size of glass plate is 10cm × 3cm.
6. the mold according to claim 1-5 for Sargassum horneri seedling preparation of specimen, it is characterised in that: the mould
The side for having main body is equipped with containing mark.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821288682.0U CN208783649U (en) | 2018-08-10 | 2018-08-10 | A kind of mold for Sargassum horneri seedling preparation of specimen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821288682.0U CN208783649U (en) | 2018-08-10 | 2018-08-10 | A kind of mold for Sargassum horneri seedling preparation of specimen |
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| Publication Number | Publication Date |
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| CN208783649U true CN208783649U (en) | 2019-04-26 |
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|---|---|---|---|
| CN201821288682.0U Expired - Fee Related CN208783649U (en) | 2018-08-10 | 2018-08-10 | A kind of mold for Sargassum horneri seedling preparation of specimen |
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| Country | Link |
|---|---|
| CN (1) | CN208783649U (en) |
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2018
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