CN103430881A - Simple method for rapidly recognizing males and females of salmon and trout juvenile fishes - Google Patents
Simple method for rapidly recognizing males and females of salmon and trout juvenile fishes Download PDFInfo
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- CN103430881A CN103430881A CN2013103656770A CN201310365677A CN103430881A CN 103430881 A CN103430881 A CN 103430881A CN 2013103656770 A CN2013103656770 A CN 2013103656770A CN 201310365677 A CN201310365677 A CN 201310365677A CN 103430881 A CN103430881 A CN 103430881A
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- sexual gland
- salmons
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- gonad
- juvenile fish
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a simple method for rapidly recognizing males and females of salmon and trout juvenile fishes. The method comprises the following steps that firstly, the gonad of one salmon and trout juvenile fish is taken out, and the pellicle of the gonad is removed so that the internal tissue of the gonad can be exposed; secondly, water is dripped on the internal organization of the gonad; the gonad is observed under a microscope, if the cells in the gonad tissue are round or approximately round, the fish is a female fish and if the cells in the gonad tissue are arranged in a dispersed shape, the fish is a male fish. By the adoption of the simple method for rapidly recognizing the males and the females of the salmon and trout juvenile fishes, a user can recognize the sex of the juvenile fishes timely, early, and rapidly and as early as possible in the process of fry cultivation and aquaculture production of the salmon and trout fishes, and the female ratio of fry is grasped; if the female ratio is high, aquaculture production can go on, and if the female ratio is low, the fry can be abandoned timely and fry culturing or purchasing can be conducted again; therefore, large economic losses and the waste of time are avoided.
Description
Technical field
The invention belongs to the marine fishery technical field, relate to the field that the Salmons sex is differentiated, relate in particular to a kind of method for quick identification of easy Salmons juvenile fish male and female.
Background technology
Propagate artificially in situation, Salmons (rainbow trout, Atlantic salmon etc.) reaches sexual maturity general 2～3 ages, and raun is generally than 1 year milter sexual maturity evening, i.e. before sexual maturity, raun is than the vegetative period of milter many 1 year, thereby can obtain the marketable fish of larger specification.Complete undoubtedly female technology, at the growth time that has to a certain degree extended cultured fishes, more is conducive to produce the marketable fish of more much specifications.
Breeding biology of fish research is the basis of fish aquaculture, and the fish sex authentication technique is the key technology of artificial propagation of fish.Differentiate fast and accurately young fish phase sex be complete female fish grow seedlings with breeding process in a vital link.Differentiate as early as possible, accurately and rapidly in time, the sex of juvenile fish, can reflect really the complete female rate of this batch of seed in seedling raising process, make in time Corresponding Countermeasures, if complete female rate rate is lower, can give up timely seed and re-start production, avoid financial resources, material resources and waste of time.
The sex of Salmons adult fish is differentiated relatively simple, and mating season, the milter head was less, and the kiss end is sharper, and lower jaw is bent upwards and is sharp hook-type, lower jaw is longer than the upper jaw, and belly does not expand, and the raun average physique is elongated, and kiss end circle is blunt, upper lower jaw is isometric, and lower abdomen expands, and gonopore is outstanding to take on a red color.
The sexual gland of young stage fish just just germinates, gonadal tissue is less, can't be differentiated with the discrimination method of adult fish, conventional method generally adopts the histologic section technology to carry out, but the histologic section technical step is loaded down with trivial details, the discriminating cycle is longer, as need are differentiated the manpower and materials that need cost more in a large number, and the number of chemical material in operating process is larger to harm.
Summary of the invention
The invention provides a kind of method for quick identification of easy Salmons juvenile fish male and female, can be used for the above juvenile fish sex of the on-the-spot body weight 10g of discriminating fast, method is simple, easy to operate, speed is fast, without specific apparatus and reagent, it is comparatively desirable juvenile fish male and female method for quick identification in a kind of seed production and breeding process.
For achieving the above object, the present invention adopts following technical proposals to be achieved:
A kind of method for quick identification of easy Salmons juvenile fish male and female, it comprises the following steps:
1) choose the above Salmons juvenile fish of 10g, take out the sexual gland of described juvenile fish;
2) sexual gland is placed on slide, its pellicle of strip off, make the interior tissue of sexual gland exposed, and spread out as far as possible;
3) drip several dripping on the interior tissue of sexual gland, gonadal tissue is immersed in water;
4) slide is positioned under microscope, observes under 100 power microscopes;
5) the gonadal tissue inner cell rounded or rotund be raun, what the gonadal tissue inner cell was that the disperse shape arranges is milter.
Wherein, described sexual gland is positioned at top, abdominal cavity near head end, be white in color, and length 1cm ~ 1.5cm.
Further, in described step 1), utilize the MS-222 anaesthetic treatment of 60 mg/L to take out sexual gland after 3～5 minutes.
Further, described step 2) in, by the fixing preservation of 10% formaldehyde for sexual gland of taking out, after fixedly completing, use 5% formaldehyde or 70% alcohol keep for a long time.
Compared with prior art, advantage of the present invention and good effect are: in Salmons seed rearing and breeding production process, utilize technical scheme of the present invention, can differentiate in time, as early as possible, accurately and rapidly the sex of juvenile fish, grasp the complete female ratio of fry, complete female rate height can continue breeding production, can give up in time seed when complete female rate is low and again grows seedlings or purchase seedling, avoids large economic loss, time waste.
The invention solves traditional histotomy complex steps, discriminating cycle long, can't on-the-spot discriminatings etc. problem, can realize the on-the-spot Salmons juvenile fish sex of differentiating fast, to the Salmons seed rearing with cultivate significant.
After reading the specific embodiment of the present invention by reference to the accompanying drawings, it is clearer that the other features and advantages of the invention will become.
The accompanying drawing explanation
Fig. 1 is positioned over the application drawing on slide by Salmons juvenile fish sexual gland.
Fig. 2 shows Salmons juvenile fish gonadal tissue inner cell shape, the sexual gland that upper figure is the juvenile fish raun, the sexual gland that figure below is the juvenile fish milter.
Embodiment
Below in conjunction with the drawings and specific embodiments, technical scheme of the present invention is described in further detail.
Embodiment 1
The method for quick identification of easy Salmons juvenile fish male and female of the present invention comprises the following steps:
1, get fresh Salmons juvenile fish (10g and more than), towel off dry juvenile fish body surface moisture, be placed on dissecting pan.Take out sexual gland, sexual gland is positioned at top, abdominal cavity near head end, is generally white, is about 1～1.5cm left and right.
2, sexual gland is placed on slide, peels off its pellicle, make interior tissue exposed (as Fig. 1), and gonadal tissue is spread out and is beneficial to microexamination as far as possible.
3, drip several dripping in the gonadal tissue of spreading out, gonadal tissue is submerged in water fully.
4, slide is positioned under microscope, observes under 100 power microscopes.
5, the gonadal tissue inner cell rounded or rotund be raun, what gonadal tissue inner cell disperse shape was arranged is milter, (as Fig. 2).
Can also utilize MS-222 (60 mg/L) anaesthetic treatment 3～5 minutes in step 1.
In step 2, the sexual gland taken out can also be preserved with 10% formaldehyde is fixing, after fixedly completing, available 5% formaldehyde or 70% alcohol keep for a long time.
Above embodiment is only in order to technical scheme of the present invention to be described, but not is limited; Although with reference to previous embodiment, the present invention is had been described in detail, for the person of ordinary skill of the art, the technical scheme that still can put down in writing previous embodiment is modified, or part technical characterictic wherein is equal to replacement; And these modifications or replacement do not make the essence of appropriate technical solution break away from the spirit and scope of the present invention's technical scheme required for protection.
Claims (4)
1. the method for quick identification of easy Salmons juvenile fish male and female is characterized in that it comprises the following steps:
1) choose the above Salmons juvenile fish of 10g, take out the sexual gland of described juvenile fish;
2) sexual gland is placed on slide, its pellicle of strip off, make the interior tissue of sexual gland exposed, and spread out as far as possible;
3) drip several dripping on the interior tissue of sexual gland, gonadal tissue is immersed in water;
4) slide is positioned under microscope, observes under 100 power microscopes;
5) the gonadal tissue inner cell rounded or rotund be raun, what the gonadal tissue inner cell was that the disperse shape arranges is milter.
2. the method for quick identification of a kind of easy Salmons juvenile fish male and female according to claim 1, it is characterized in that: described sexual gland is positioned at top, abdominal cavity near head end, be white in color, length 1cm ~ 1.5cm.
3. the method for quick identification of a kind of easy Salmons juvenile fish male and female according to claim 1, is characterized in that: in described step 1), utilize the MS-222 anaesthetic treatment of 60 mg/L to take out sexual gland after 3～5 minutes.
4. the method for quick identification of a kind of easy Salmons juvenile fish male and female according to claim 1, it is characterized in that: described step 2), by the fixing preservation of 10% formaldehyde for sexual gland of taking out, after fixedly completing, use 5% formaldehyde or 70% alcohol keep for a long time.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2013103656770A CN103430881A (en) | 2013-08-21 | 2013-08-21 | Simple method for rapidly recognizing males and females of salmon and trout juvenile fishes |
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| CN2013103656770A CN103430881A (en) | 2013-08-21 | 2013-08-21 | Simple method for rapidly recognizing males and females of salmon and trout juvenile fishes |
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| CN103430881A true CN103430881A (en) | 2013-12-11 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106282401A (en) * | 2016-11-14 | 2017-01-04 | 中国水产科学研究院 | Salmon Squaliobarbus ourriculus general SNP marker combination and application |
| CN107047394A (en) * | 2017-06-09 | 2017-08-18 | 苏州大学 | A kind of zander is female, milter identification device and using the device discrimination method |
Citations (4)
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|---|---|---|---|---|
| JPH0276528A (en) * | 1988-09-13 | 1990-03-15 | Fujitsu Autom Kk | Fishes-sexing apparatus and method |
| RU2264093C2 (en) * | 2003-03-18 | 2005-11-20 | Фомин Александр Владимирович | Method for detecting sex in humpbacked salmon oncorhynchus gorbuscha |
| CN101803578A (en) * | 2010-03-30 | 2010-08-18 | 中国水产科学研究院东海水产研究所 | Convenient sex estimation method for juvenile flounder |
| JP5101404B2 (en) * | 2007-06-20 | 2012-12-19 | 浜松ホトニクス株式会社 | Fish sex determination apparatus and fish sex determination method |
-
2013
- 2013-08-21 CN CN2013103656770A patent/CN103430881A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0276528A (en) * | 1988-09-13 | 1990-03-15 | Fujitsu Autom Kk | Fishes-sexing apparatus and method |
| RU2264093C2 (en) * | 2003-03-18 | 2005-11-20 | Фомин Александр Владимирович | Method for detecting sex in humpbacked salmon oncorhynchus gorbuscha |
| JP5101404B2 (en) * | 2007-06-20 | 2012-12-19 | 浜松ホトニクス株式会社 | Fish sex determination apparatus and fish sex determination method |
| CN101803578A (en) * | 2010-03-30 | 2010-08-18 | 中国水产科学研究院东海水产研究所 | Convenient sex estimation method for juvenile flounder |
Non-Patent Citations (5)
| Title |
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| ANDREAS MAGERHANS ET AL: "Selection experiments to alter the sex ratio in rainbow trout (Oncorhynchus mykiss)", 《AQUACULTURE》, vol. 306, 15 August 2010 (2010-08-15), pages 63 - 67, XP027156515 * |
| BRUNO MENU ET AL: "A shortcut method for sexing juvenile European sea", 《AQUACULTURE RESEARCH》, vol. 36, no. 1, 31 January 2005 (2005-01-31), pages 41 - 44 * |
| 赵维信等: "大西洋鲑性腺分化及热休克的影响", 《水产学杂志》, vol. 7, no. 2, 30 November 1994 (1994-11-30), pages 1 - 6 * |
| 赵维信等: "虹鳟性腺分化的研究", 《鲑鳟渔业》, vol. 3, no. 2, 31 December 1990 (1990-12-31) * |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106282401A (en) * | 2016-11-14 | 2017-01-04 | 中国水产科学研究院 | Salmon Squaliobarbus ourriculus general SNP marker combination and application |
| CN106282401B (en) * | 2016-11-14 | 2019-05-28 | 中国水产科学研究院 | Salmon trout general SNP marker combination and its application |
| CN107047394A (en) * | 2017-06-09 | 2017-08-18 | 苏州大学 | A kind of zander is female, milter identification device and using the device discrimination method |
| CN107047394B (en) * | 2017-06-09 | 2022-10-14 | 苏州大学 | Female and male zander identification device and identification method using same |
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Application publication date: 20131211 |