CN101803578A - Convenient sex estimation method for juvenile flounder - Google Patents
Convenient sex estimation method for juvenile flounder Download PDFInfo
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- CN101803578A CN101803578A CN201010136435A CN201010136435A CN101803578A CN 101803578 A CN101803578 A CN 101803578A CN 201010136435 A CN201010136435 A CN 201010136435A CN 201010136435 A CN201010136435 A CN 201010136435A CN 101803578 A CN101803578 A CN 101803578A
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- lefteye flounder
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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Abstract
The invention relates to a convenient sex estimation method for a juvenile flounder, which comprises the following steps: (1) weighing a juvenile flounder, fixing the part of the abdomen including the gonad in a Davision's AFA fixative, processing for 24 hours, and preserving in ethanol of which the concentration is 70%; (2) placing the fixed abdomen of the juvenile flounder under the anatomical lens, peeling off the epidermis and muscle, and taking off the gonadal tissue; and (3) placing the gonadal tissue under the anatomical lens, and observing the shape of the gonadal tissue included in the gonad epidermis, wherein the gonadal tissue of a female flounder is bigger and is in the shape of a heart, while the gonadal tissue of a male flounder is smaller and slender and is in the shape of a lengthened D. The invention has the advantages of convenient and simple operation, low cost, and stable and reliable result, and is applicable to the researches on the propagation and the growth of multiple fishes, such as flounders and the like.
Description
Technical field
The invention belongs to the judgement field of left-eyed flounder sex, particularly be involved in sex determination's method of a kind of easy lefteye flounder juvenile fish.
Background technology
The sex determination is researching fish breeding and the basis of growing, and the sexual gland of fish is divided into spermary and ovary, has different functions in the breeding of fish.In fish research, breeding growth, internal secretion adjusting, external environment and the chemical substance etc. that comprise fish all need to judge its sex to the breedings of fish and physiological effect etc.Left-eyed flounders such as lefteye flounder have the characteristics of female individuals growth rate greater than male, and in order to improve productivity effect, unisexuality breeding technique such as gynogenesis, hormone induction are feminized and temperature Sex determination etc. grows up gradually.And in these researchs, all need to judge in a large number the sex of juvenile fish, carry out sex ratio and calculate to study artificial sex-controlled effect.Equally, when left-eyed flounder being carried out endocrine regulation research, also need at first to determine its sex composition.
Because the sexual gland of fish juvenile fish is undeveloped mature also, gonadal tissue is less, is tiny present situation, can not with the naked eye differentiate sex, and generally, the sex determination mainly relies on the histologic section technology to carry out.But histologic section technology operating procedure is more, must spend more manpower and materials as a large amount of judgements, and the chemical substances such as dimethylbenzene in its operating process have bigger harm to operator ' s health.
Summary of the invention
The problem that the present invention need solve has provided a kind of easy lefteye flounder juvenile fish sex determination method.This invention flow process is simple, easy to operate, and whole process is consuming time few, the result is reliable and stable, need not special or expensive instrument, and cost is few, required reagent is few, reduces the multiple toxic chemical substance that is contacted in operating personnel's histologic section process, operating personnel is injured minimum.
A kind of easy lefteye flounder juvenile fish sex determination method of the present invention comprises:
(1) gets fresh lefteye flounder juvenile fish, dissect after the weighing and the belly that will contain gonadal tissue is fixed in Davision ' the s alcoholformalacetic fixative, behind fixing 24h, use 70% ethanol instead and keep for a long time;
(2) fixing lefteye flounder belly is placed on the slide, is placed under the anatomical lens, peel off its epidermis and muscular tissue, take out complete gonadal tissue;
(3) end with the close kidney of sexual gland is put in anatomical lens visual field central authorities, smooths its epidermis part gently with dissecting needle and tweezers;
(4) shape of gonadal tissue in the nearly kidney one end sexual gland part epidermis of observation is according to its sex of different decision of shape.
Davision ' s alcoholformalacetic fixative is prepared by 6: 3: 1 by volume ratio of absolute ethyl alcohol, formaldehyde, glacial acetic acid in the described step (1).
In the described step (4) according to its sex method of different decision of shape for if sexual gland is big and be " heart " shape then for female, if sexual gland is elongated and be elongated " D " shape then for male.
The present invention is according to the gonadal tissue structure of left-eyed flounders such as lefteye flounder and the difference of shape thereof, design efficiently sex determination's method without the histologic section technology, prove through repetition test and histologic section, described method can be judged the sex of the lefteye flounder juvenile fish of total length more than 6.0cm after the Sex Differentiation exactly, the present invention can be used for the researchs such as neuroendocrine regulation, unisexuality cultivation and the regulation and control of envirment factor sex of left-eyed flounders such as lefteye flounder, has extensive applicability.
Beneficial effect
(1) flow process of the present invention is simple, and is easy to operate, and whole process is consuming time few, and the result is reliable and stable;
(2) need not special or expensive instrument, cost is few, and required reagent is few, reduces the multiple toxic chemical substance that is contacted in operating personnel's histologic section process, operating personnel is injured minimum.
Description of drawings
The lefteye flounder gonadal tissue that Fig. 1 takes out down for anatomical lens;
Fig. 2 is female lefteye flounder " heart " shape sexual gland;
Fig. 3 is male Japanese flounders " D " shape sexual gland.
Embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Embodiment 1
(1) gets fresh lefteye flounder juvenile fish, weighing (total length 6.9cm, 2.78g), dissect and the belly that will contain gonadal tissue is fixed in Davision ' the s alcoholformalacetic fixative (absolute ethyl alcohol, formaldehyde, glacial acetic acid are according to volume ratio preparation in 6: 3: 1), behind fixing 24h, use 70% ethanol instead and keep for a long time;
(2) get fixing lefteye flounder belly and be placed on the slide, be placed under the anatomical lens, peel off its epidermis and muscular tissue, take out complete gonadal tissue;
(3) end with the close kidney of sexual gland is put in anatomical lens visual field central authorities, smooths its epidermis part gently with dissecting needle and tweezers;
(4) shape of gonadal tissue in the nearly kidney one end sexual gland part epidermis of observation is according to its sex of different decision of shape; Sexual gland is elongated and be elongated " D " shape, judges that its sex is male.This gonadal tissue is done routine paraffin wax section, and the dyeing back is observed, and its sexual gland of empirical tests is male, and it is elongated bar-shaped that spermary is, and the result is consistent with the anatomical lens microscopy.
Embodiment 2
(1) gets fresh lefteye flounder juvenile fish, weighing (total length 7.5cm, 3.12g), dissect and the belly that will contain gonadal tissue is fixed in Davision ' the s alcoholformalacetic fixative (absolute ethyl alcohol, formaldehyde, glacial acetic acid are according to volume ratio preparation in 6: 3: 1), behind fixing 24h, use 70% ethanol instead and keep for a long time;
(2) get fixing lefteye flounder belly and be placed on the slide, be placed under the anatomical lens, peel off its epidermis and muscular tissue, take out complete gonadal tissue;
(3) end with the close kidney of sexual gland is put in anatomical lens visual field central authorities, smooths its epidermis part gently with dissecting needle and tweezers;
(4) shape of gonadal tissue in the nearly kidney one end sexual gland part epidermis of observation is according to its sex of different decision of shape; Sexual gland is big and be " heart " shape, judges that its sex is female.
This gonadal tissue is done the routine paraffin wax section, and the dyeing back is observed, and its sexual gland of empirical tests is female, and ovary is bigger, the shape basically identical that ovary shape in the histologic section and anatomical lens are observed down.
Claims (3)
1. easy lefteye flounder juvenile fish sex determination method comprises:
(1) gets fresh lefteye flounder juvenile fish, dissect after the weighing and the belly that will contain gonadal tissue is fixed in Davision ' the s alcoholformalacetic fixative, behind fixing 24h, use 70% ethanol instead and keep for a long time;
(2) fixing lefteye flounder belly is placed on the slide, is placed under the anatomical lens, peel off its epidermis and muscular tissue, take out complete gonadal tissue;
(3) end with the close kidney of sexual gland is put in anatomical lens visual field central authorities, smooths its epidermis part gently with dissecting needle and tweezers;
(4) shape of gonadal tissue in the nearly kidney one end sexual gland part epidermis of observation is according to its sex of different decision of shape.
2. a kind of easy lefteye flounder juvenile fish sex determination method according to claim 1 is characterized in that: Davision ' s alcoholformalacetic fixative is prepared by 6: 3: 1 by volume ratio of absolute ethyl alcohol, formaldehyde, glacial acetic acid in the described step (1).
3. a kind of easy lefteye flounder juvenile fish sex determination method according to claim 1, it is characterized in that: in the described step (4) according to its sex method of different decision of shape for if sexual gland is big and be " heart " shape then for female, if sexual gland is elongated and be elongated " D " shape then for male.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2010101364350A CN101803578B (en) | 2010-03-30 | 2010-03-30 | Convenient sex estimation method for juvenile flounder |
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| CN2010101364350A CN101803578B (en) | 2010-03-30 | 2010-03-30 | Convenient sex estimation method for juvenile flounder |
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| CN101803578A true CN101803578A (en) | 2010-08-18 |
| CN101803578B CN101803578B (en) | 2012-06-20 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102217564A (en) * | 2011-04-21 | 2011-10-19 | 中国水产科学研究院东海水产研究所 | Juvenile pomfret gonad fixing and obtaining method |
| CN102221484A (en) * | 2011-04-21 | 2011-10-19 | 中国水产科学研究院东海水产研究所 | Gonad slicing method for pomfret larvae juvenile fish |
| CN103430881A (en) * | 2013-08-21 | 2013-12-11 | 山东省海水养殖研究所 | Simple method for rapidly recognizing males and females of salmon and trout juvenile fishes |
| CN103940647A (en) * | 2014-04-02 | 2014-07-23 | 浙江万里学院 | Manufacture method of continuous paraffin sections for gonad of pelodiscus sinensis in embryonic period and application of manufacture method in sex determination |
| CN103975886A (en) * | 2014-06-03 | 2014-08-13 | 广西壮族自治区水产科学研究院 | Method for judging sex of trachinotus ovatus at early stage |
| CN107822670A (en) * | 2017-09-22 | 2018-03-23 | 湖北大学 | A kind of fast and accurately spider enteron aisle anatomic method |
-
2010
- 2010-03-30 CN CN2010101364350A patent/CN101803578B/en not_active Expired - Fee Related
Non-Patent Citations (4)
| Title |
|---|
| 《中国水产科学》 20070930 王文君等 石鲽仔、幼鱼性腺发育的组织学观察 第843-847页 1-3 第14卷, 第5期 2 * |
| 《大连水产学院学报》 20081231 孙朝徽等 养殖牙鲆性腺分化的组织学观察 第451-454页 1-3 第23卷, 第6期 2 * |
| 《海洋科学》 20091231 孙鹏等 牙鲆性腺分化的组织学研究 第53-58页 1-3 第33卷, 第3期 2 * |
| 《渔业科学进展》 20091231 马健等 黄海鳀鱼的卵巢发育 第7-17页 1-3 第30卷, 第6期 2 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102217564A (en) * | 2011-04-21 | 2011-10-19 | 中国水产科学研究院东海水产研究所 | Juvenile pomfret gonad fixing and obtaining method |
| CN102221484A (en) * | 2011-04-21 | 2011-10-19 | 中国水产科学研究院东海水产研究所 | Gonad slicing method for pomfret larvae juvenile fish |
| CN102217564B (en) * | 2011-04-21 | 2013-03-20 | 中国水产科学研究院东海水产研究所 | Juvenile pomfret gonad fixing and obtaining method |
| CN103430881A (en) * | 2013-08-21 | 2013-12-11 | 山东省海水养殖研究所 | Simple method for rapidly recognizing males and females of salmon and trout juvenile fishes |
| CN103940647A (en) * | 2014-04-02 | 2014-07-23 | 浙江万里学院 | Manufacture method of continuous paraffin sections for gonad of pelodiscus sinensis in embryonic period and application of manufacture method in sex determination |
| CN103975886A (en) * | 2014-06-03 | 2014-08-13 | 广西壮族自治区水产科学研究院 | Method for judging sex of trachinotus ovatus at early stage |
| CN103975886B (en) * | 2014-06-03 | 2016-04-06 | 广西壮族自治区水产科学研究院 | A kind of method that egg-shaped pompano early sex judges |
| CN107822670A (en) * | 2017-09-22 | 2018-03-23 | 湖北大学 | A kind of fast and accurately spider enteron aisle anatomic method |
| CN107822670B (en) * | 2017-09-22 | 2020-03-10 | 湖北大学 | Rapid and accurate spider intestinal tract dissection method |
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| Publication number | Publication date |
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| CN101803578B (en) | 2012-06-20 |
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