CN103975886A - Method for judging sex of trachinotus ovatus at early stage - Google Patents
Method for judging sex of trachinotus ovatus at early stage Download PDFInfo
- Publication number
- CN103975886A CN103975886A CN201410241717.5A CN201410241717A CN103975886A CN 103975886 A CN103975886 A CN 103975886A CN 201410241717 A CN201410241717 A CN 201410241717A CN 103975886 A CN103975886 A CN 103975886A
- Authority
- CN
- China
- Prior art keywords
- egg
- sexual gland
- sex
- shaped pompano
- shaped
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Farming Of Fish And Shellfish (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a method for judging the sex of trachinotus ovatus at an early stage. Young trachinotus ovatus is selected, the left body wall is cut off, sex gland is found out, characteristics of the sex gland are observed in situ, the sex gland is taken out and dissected, the characteristics of the dissection face of the sex gland are observed, and the sex of the trachinotus ovatus is judged by analyzing the characteristics of the sex gland According to the method, the instrument is simple, operation is rapid, the result is accurate, toxic chemical agents are not used, the method for judging the sex of the trachinotus ovatus at the early stage is convenient to carry out efficient, and reliable materials can be provided for molecular marker research for further searching for sex specialty of the trachinotus ovatus.
Description
Technical field
The invention belongs to the fish sex authenticate technology in aquatic biological technical field, more particularly, the present invention relates to a kind of method that egg-shaped pompano early sex is judged.
Background technology
Egg-shaped pompano (
trachinotus ovatus), being commonly called as golden silvery pomfret, yellow cured silvery pomfret, belongs to Perciformes, Scad section.The egg-shaped pompano flesh of fish is delicate, and delicious flavour, is of high nutritive value, and is famous and precious edible fishes, is received by the market.Simultaneously egg-shaped pompano growth rate is fast, premunition is strong, output is high, profitable, be the breed variety that raiser likes very much.But the egg-shaped pompano sexual maturity time is long, generally need 4 to 5 years, and in its whole life process of egg-shaped pompano, female milter all do not possess the special resemblance of sex.Therefore, culturist's male and female that are not easily distinguishable in carrying out parent fish rearing and hastening parturition, the production cost that has increased parent fish rearing and hastened parturition.And along with egg-shaped pompano industrialized development, breed breeding and scale seed breeding, all need the parent that a large amount of sexes are clear and definite to carry out artificial propagation and family foundation.Find the special molecular labeling of sex, the molecular biology sex determination method of setting up PCR-based is the effective means of carrying out the qualification of egg-shaped pompano early sex, and carries out the screening of sex specific molecular marker, needs the individuality of sex determination as screening material.
Lacking under egg-shaped pompano molecular biology sex determination method condition, be tested and appraised its sexual gland feature and can judge egg-shaped pompano sex.Current research juvenile fish sexual gland feature is carried out prejudgementing character method for distinguishing and is mainly contained supercritical ultrasonics technology and method of tableting, and the Gonadal Morphology arriving according to the observation and structure, can judge that sexual gland is as spermary or ovary, and then judge juvenile fish sex.As Chinese patent 200810208110.1(application number) a kind of method for identifying sex of sturgeon by ultrasonic waves disclosed, the method adopts supersonic reflectoscope qualification to be developed to the sturgeon sexual gland of II phase and identifies sex, before ultrasonic examination, sturgeon cultivates period through the low temperature of 2 months, sturgeon stops throwing something and feeding two weeks before checking, when ultrasonic examination, more clear and typical sexual gland ultrasonic examination image is observed in preservation, compares to differentiate the sex of sturgeon with the ultrasonography feature of different development stage spermary and ovary.Chinese patent 201310466149.4(application number) a kind of discriminating hybridization parr of yellow catfish method for distinguishing disclosed, the method is used the dye method of the purple dyeing of an alcohol crystal of live body sexual gland compressing tablet one aceto-camine, identifies that individual body long size is more than 3 centimetres to hybridize yellow cartfish sex.Supercritical ultrasonics technology research juvenile fish sexual gland, juvenile fish need to pass through special processing, and ultrasound measuring instrument is expensive, and testing staff's technical requirement is high, is not suitable for numerous raisers' demand.Method of tableting research juvenile fish sexual gland, need to configure multiple comparatively complicated chemical reagent, and operating procedure is many, and technical requirement is stricter, and need to be equipped with biomicroscope, is not suitable for the qualification of a large amount of samples.
Also not about bibliographical information and the patent of invention of egg-shaped pompano sex appraisal method, cause very large difficulty with the work of setting up of seed selection family to hastening parturition in egg-shaped pompano seed breeding at present, be unfavorable for the development of egg-shaped pompano aquaculture industry.Therefore, development one simply, egg-shaped pompano sex appraisal method fast and accurately, can be the special molecule marking research of searching egg-shaped pompano sex reliable material is provided, particularly important to setting up egg-shaped pompano molecular biology sex appraisal method, the active demand of industry development especially.
Summary of the invention
The object of the invention is to overcome prior art deficiency, a kind of method that provides egg-shaped pompano early sex to judge, gets children's egg-shaped pompano in age, takes out complete one-sided sexual gland from viscera tissue, judges egg-shaped pompano sex by comparing sexual gland feature.
The present invention is achieved in that
The method that egg-shaped pompano early sex is judged, comprises the following steps:
(1) get children's egg-shaped pompano in age, cut off body wall, expose internal organ.
(2) find left and right sexual gland from cloacal aperture toward abdominal cavity direction, home position observation sexual gland position, shape, color and size characteristic.
(3) divest sexual gland surrounding tissue, take out complete one-sided sexual gland, separate plane sexual gland, observe sexual gland dissection surface architectural feature.
(4) the sexual gland feature of observing according to step (2) and (3) is judged egg-shaped pompano sex: sexual gland is positioned at the both sides, back of fish glue, be shaped as elongated wire, milky, small volume, what dissection surface was solid construction is spermary, judge that egg-shaped pompano is as male, or sexual gland is positioned at the both sides, back of fish glue, be close to body wall and kidney, be shaped as thin ends and thick middle, faint yellow, volume is larger, dissection surface be hollow-core construction for ovary, judge that egg-shaped pompano is as female.
As further restriction of the present invention, in step (1), described children egg-shaped pompano in age be one age egg-shaped pompano.
As further restriction of the present invention, in step (1), described body wall is left side body wall.
As further restriction of the present invention, in step (2), described in be viewed as and visually observe.
As further restriction of the present invention, in step (3), described dissection sexual gland is from middle part transversely cutting by sexual gland.
As further restriction of the present invention, in step (3), described in be viewed as and visually observe and dissect sem observation.
Compared with prior art, tool of the present invention has the following advantages:
(1) one age egg-shaped pompano get final product Sex estimation, judgement period early, can be next step and sets up egg-shaped pompano early sex molecular biological variety identification method research material is provided, as early as possible for parent fish rearing provides technical support.The direct Sex estimation of passability gland form after sexual maturity individual dissection, but cost costliness.
(2) sexual gland separation process is simple, quick, and scissors or scalpel can complete, and is applicable to numerous raisers' operation.
(3) requirement of sexual gland observation of characteristics method and technology is low, and naked eyes or anatomical lens can carry out, and have improved observation efficiency.
(4) the sexual gland feature difference of different sexes is obvious, and decision method is easy, result is accurate.
(5) the method without any need for chemical reagent, do not need expensive apparatus, cost is extremely low, be applicable to the quick judgement demand of extensive sample, and traditional paraffin section observation of cell form Sex estimation that passes through, need loaded down with trivial details experimental procedure, and need use the toxic reagent such as paraformaldehyde, dimethylbenzene.
Brief description of the drawings
Fig. 1 is the method process chart that a kind of egg-shaped pompano early sex of the present invention is judged.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in more detail.
embodiment 1
1, get one age egg-shaped pompano, cut off left side body wall, expose viscera tissue.
2, find enteron aisle and left and right sexual gland from cloacal aperture toward abdominal cavity direction, home position observation sexual gland is positioned at the both sides, back of fish glue, and sexual gland is elongated wire, milky, small volume.
3, carefully peel off except extragonadal other viscera tissue, take out complete one-sided sexual gland, from middle part transversely cutting, visually observe and dissect Microscopic observation, be solid construction.
4, judge that sexual gland is as spermary, egg-shaped pompano is male.
In order to verify the accuracy of the method, carry out following slicing experiment:
Take out opposite side sexual gland and fix 24h in 4% PFA solution, after the ethanol dehydration of 70% ~ 100% gradient, carry out routine paraffin wax section and HE dyeing.In optical microphotograph Microscopic observation gonadal cell form, be spermary tissue, there are typical smart folliculus structure, spermatogonium and each phase spermatocyte.
The present embodiment is verified 30 fishes altogether, average weight 0.62 ± 0.057kg, long 24.56 ± the 1.143cm of average body, wherein utilizing the inventive method checking to have 18 fishes is that male (sexual gland is elongated wire, milky, small volume, section is solid construction), through section checking, male fish is 18, form determination rate of accuracy 100% again.
embodiment 2
1, get one age egg-shaped pompano, cut off left side body wall, expose viscera tissue.
2, find enteron aisle and left and right sexual gland from cloacal aperture toward abdominal cavity direction, home position observation sexual gland is positioned at the both sides, back of fish glue, is close to body wall and kidney, and sexual gland shape is thin ends and thick middle, faint yellow, and volume is larger.
3, carefully peel off except extragonadal other viscera tissue, take out complete one-sided sexual gland, from middle part transversely cutting, visually observe and dissect Microscopic observation, be hollow-core construction, under microscope, visible ovum cavity wall raw graininess egg mother cell.
4, judge that sexual gland is as ovary, egg-shaped pompano is female.
In order to verify the accuracy of the method, carry out following slicing experiment:
Take out opposite side sexual gland and fix 24h in 4% PFA solution, after the ethanol dehydration of 70% ~ 100% gradient, carry out routine paraffin wax section and HE dyeing.In optical microphotograph Microscopic observation sexual gland cross section cellular morphology, be ovary tissue, be ovarian cavity in the middle of ovary, ovary leaflet tight be born in ovarian cavity wall.A large amount of distribution first phase egg mother cells in ovary leaflet.
The present embodiment is verified 30 fishes altogether, average weight 0.66 ± 0.059kg, long 24.66 ± the 1.027cm of average body, wherein utilizing the inventive method checking to have 15 fishes is that female (sexual gland shape is thin ends and thick middle, faint yellow, and volume is larger, section is hollow-core construction), through section checking, female fish is 15, form determination rate of accuracy 100% again.
Claims (6)
1. the method that egg-shaped pompano early sex is judged, is characterized in that, comprises the following steps:
(1) get children's egg-shaped pompano in age, cut off body wall, expose internal organ;
(2) find left and right sexual gland from cloacal aperture toward abdominal cavity direction, home position observation sexual gland position, shape, color and size characteristic;
(3) divest sexual gland surrounding tissue, take out complete one-sided sexual gland, dissect sexual gland, observe sexual gland dissection surface architectural feature;
(4) the sexual gland feature of observing according to step (2) and (3) is judged egg-shaped pompano sex: sexual gland is positioned at the both sides, back of fish glue, is shaped as elongated wire, white, and what dissection surface was solid construction is spermary, judges that egg-shaped pompano is as male; Or sexual gland is positioned at the both sides, back of fish glue, be close to body wall and kidney, be shaped as thin ends and thick middle, yellowly, what dissection surface was hollow-core construction is ovary, judges that egg-shaped pompano is as female.
2. the method that a kind of egg-shaped pompano early sex according to claim 1 is judged, is characterized in that: in step (1), described children's egg-shaped pompano in age be one age egg-shaped pompano.
3. the method that a kind of egg-shaped pompano early sex according to claim 1 is judged, is characterized in that: in step (1), described body wall is left side body wall.
4. the method that a kind of egg-shaped pompano early sex according to claim 1 is judged, is characterized in that: in step (2), described in be viewed as and visually observe.
5. the method that a kind of egg-shaped pompano early sex according to claim 1 is judged, is characterized in that: in step (3), described dissection is from middle part transversely cutting by sexual gland.
6. the method that a kind of egg-shaped pompano early sex according to claim 1 is judged, is characterized in that: in step (3), described in be viewed as and visually observe and anatomical lens is observed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410241717.5A CN103975886B (en) | 2014-06-03 | 2014-06-03 | A kind of method that egg-shaped pompano early sex judges |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410241717.5A CN103975886B (en) | 2014-06-03 | 2014-06-03 | A kind of method that egg-shaped pompano early sex judges |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103975886A true CN103975886A (en) | 2014-08-13 |
| CN103975886B CN103975886B (en) | 2016-04-06 |
Family
ID=51268263
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410241717.5A Active CN103975886B (en) | 2014-06-03 | 2014-06-03 | A kind of method that egg-shaped pompano early sex judges |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103975886B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110521637A (en) * | 2019-08-26 | 2019-12-03 | 中国水产科学研究院南海水产研究所 | A kind of egg-shaped pompano family full-sibs construction method |
| CN111088370A (en) * | 2020-01-20 | 2020-05-01 | 中国水产科学研究院南海水产研究所 | Sex-specific molecular marker primer for Trachinotus ovatus, identification method and application of sex-specific molecular marker primer |
| CN111206082A (en) * | 2020-02-18 | 2020-05-29 | 中国水产科学研究院南海水产研究所 | Sex-specific molecular marker primer and kit for Trachinotus ovatus and application of sex-specific molecular marker primer and kit |
| CN111378731A (en) * | 2020-02-18 | 2020-07-07 | 中国水产科学研究院南海水产研究所 | Sex-specific molecular marker primer for Trachinotus ovatus and application of sex-specific molecular marker primer |
| CN112239779A (en) * | 2020-10-21 | 2021-01-19 | 中国水产科学研究院南海水产研究所 | Primer and kit for quickly identifying sex of egg-shaped pompano and application of primer and kit |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000004708A (en) * | 1998-06-23 | 2000-01-11 | Kobe Steel Ltd | Device for distinguishing male and female of fish |
| CN101803578A (en) * | 2010-03-30 | 2010-08-18 | 中国水产科学研究院东海水产研究所 | Convenient sex estimation method for juvenile flounder |
| JP2011505794A (en) * | 2007-11-12 | 2011-03-03 | フンダシオン アッジ−アッジ フンダシオ | Method and apparatus for determining the sex of a fish |
| CN102217564A (en) * | 2011-04-21 | 2011-10-19 | 中国水产科学研究院东海水产研究所 | Juvenile pomfret gonad fixing and obtaining method |
-
2014
- 2014-06-03 CN CN201410241717.5A patent/CN103975886B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000004708A (en) * | 1998-06-23 | 2000-01-11 | Kobe Steel Ltd | Device for distinguishing male and female of fish |
| JP2011505794A (en) * | 2007-11-12 | 2011-03-03 | フンダシオン アッジ−アッジ フンダシオ | Method and apparatus for determining the sex of a fish |
| CN101803578A (en) * | 2010-03-30 | 2010-08-18 | 中国水产科学研究院东海水产研究所 | Convenient sex estimation method for juvenile flounder |
| CN102217564A (en) * | 2011-04-21 | 2011-10-19 | 中国水产科学研究院东海水产研究所 | Juvenile pomfret gonad fixing and obtaining method |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110521637A (en) * | 2019-08-26 | 2019-12-03 | 中国水产科学研究院南海水产研究所 | A kind of egg-shaped pompano family full-sibs construction method |
| CN111088370A (en) * | 2020-01-20 | 2020-05-01 | 中国水产科学研究院南海水产研究所 | Sex-specific molecular marker primer for Trachinotus ovatus, identification method and application of sex-specific molecular marker primer |
| CN111088370B (en) * | 2020-01-20 | 2022-06-21 | 中国水产科学研究院南海水产研究所 | Sex-specific molecular marker primer for Trachinotus ovatus, identification method and application of sex-specific molecular marker primer |
| CN111206082A (en) * | 2020-02-18 | 2020-05-29 | 中国水产科学研究院南海水产研究所 | Sex-specific molecular marker primer and kit for Trachinotus ovatus and application of sex-specific molecular marker primer and kit |
| CN111378731A (en) * | 2020-02-18 | 2020-07-07 | 中国水产科学研究院南海水产研究所 | Sex-specific molecular marker primer for Trachinotus ovatus and application of sex-specific molecular marker primer |
| CN112239779A (en) * | 2020-10-21 | 2021-01-19 | 中国水产科学研究院南海水产研究所 | Primer and kit for quickly identifying sex of egg-shaped pompano and application of primer and kit |
| CN112239779B (en) * | 2020-10-21 | 2023-08-18 | 中国水产科学研究院南海水产研究所 | Primer and kit for rapidly identifying sex of trachinotus ovatus and application of primer and kit |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103975886B (en) | 2016-04-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103975886B (en) | A kind of method that egg-shaped pompano early sex judges | |
| SO et al. | Semen Guality Traits of Seven Strain of Chickens Raised in the Humid Tropics | |
| CN104704360B (en) | Fowl embryo gender, vigor and/or stage of development determine in egg | |
| CN106719114B (en) | Hybrid between Fugu obscurus and Fugu rubripes and its production process | |
| Ferreri et al. | Validation of macroscopic maturity stages according to microscopic histological examination for European anchovy | |
| KB et al. | Reproductive biology, length–weight relationship and condition factor of the African snake head, Parachanna obscura, from River Oshun, South-west Nigeria | |
| Scholtz et al. | Effects of different microalgal feeds and their influence on larval development in the white-spined sea urchin Tripneustes gratilla | |
| Kobayashi et al. | Gonadal morphogenesis and sex differentiation in cultured chub mackerel, Scomber japonicus | |
| Wang et al. | The development process and seasonal changes of the gonad in Octopus ocellatus Gray off the coast of Qingdao, Northeast China | |
| Jeuthe et al. | DNA fragmentation and membrane integrity in sperm of farmed Arctic charr (Salvelinus alpinus) | |
| CN102090360B (en) | High-efficient induction method of tetraploid cynoglossus semilaevis fish fries | |
| Wang et al. | Adaptive and plastic variation in growth and maturation of the cutlassfish Trichiurus japonicus in the subtropical Pacific Ocean | |
| Cabrita et al. | Technologies and strategies for ex situ conservation of aquatic organisms: the role of cryopreservation in long-term management | |
| De Souza et al. | Artificial Selection and Size at First Sexual Maturity of Perna perna Mussels (Linnaeus, 1758) in Southeastern Brazil | |
| Nurlita et al. | Detection of the existence of viral nervous necrosis on fry cantang grouper at rain season | |
| Sogabe et al. | Ovarian structure and mode of egg production in the seaweed pipefish Syngnathus schlegeli (Syngnathidae) | |
| CN102119674A (en) | Cynoglossus semilaevis triploid fry mass inducing method | |
| Fatihah et al. | Gonad maturation of giant freshwater prawn, Macrobrahium rosenbergii at the bay of Padas River, Beaufort, Sabah, Malaysia | |
| CN102586392A (en) | Quality detection method for unfertilized egg of turbo | |
| CN114182024B (en) | Molecular biological method for quantifying fish ovary development number by using 5S rRNA | |
| CN114214427B (en) | Triploid oyster ploidy identification and analysis method of genetic material source thereof | |
| Sari et al. | The relationship of body length and ratio pappilla with sex in gobi fish (Sicyopterus macrostetholepis) | |
| Bencsik et al. | Triploidy determination in rainbow trout (Oncorhynchus mykiss) based on erythrocytes dimensions. | |
| RU2437282C2 (en) | Method to assess and preserve morphometric characteristics of biological species in aquaculture | |
| Brown | Ovarian morphology, oogenesis, and changes through the annual reproductive cycle of the female blue crab, Callinectes sapidus Rathbun, in Tampa Bay |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |