CN107047394A - A kind of zander is female, milter identification device and using the device discrimination method - Google Patents

A kind of zander is female, milter identification device and using the device discrimination method Download PDF

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Publication number
CN107047394A
CN107047394A CN201710434158.3A CN201710434158A CN107047394A CN 107047394 A CN107047394 A CN 107047394A CN 201710434158 A CN201710434158 A CN 201710434158A CN 107047394 A CN107047394 A CN 107047394A
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China
Prior art keywords
zander
flexible pipe
tube body
extraction tube
parent
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CN201710434158.3A
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CN107047394B (en
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凌去非
李彩娟
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Suzhou University
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Suzhou University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The invention discloses a kind of zander is female, milter identification device and the discrimination method using the device, including sampling flexible pipe, transition flexible pipe, extraction tube;The extraction tube includes extracting tube body, pull bar;The extraction tube body one end open;The pull bar is from tube body is extracted in the opening insertion;The pull bar can contact the extraction tube body madial wall and carry out piston movement;Described not opening one end of tube body of extracting is provided with adapter;The adapter is connected with the extraction tube body;The transition flexible pipe one is terminated with adapter, and one is terminated with sampling flexible pipe.Compared with other resolution zander property method for distinguishing, the features such as this method has time saving, laborsaving, reliable and stable;Improve zander sex identification efficiency, and the degree of accuracy 100%, great application value.

Description

A kind of zander is female, milter identification device and using the device discrimination method
Technical field
The invention belongs to zander artificial propagation field, and in particular to a kind of zander is female, milter identification device and utilize the dress The discrimination method put.
Background technology
Zander is distributed across the Main Commercial Fishes of Europe and Xinjiang of China Eerqisihe River water system, and the fish thermophilic is wide(0 ℃-33℃), Salt And Alkali Tolerance.Pierce few between fine and tender taste, flesh, suitable for processing.Nutritive value is high, and its protein content exceedes 19.94%, compare mandarin fish(17.56%)Height, 5 kinds cause fresh amino acid content to be 9.31%, higher than rainbow trout(9.11%), have " fresh-water fishes king " it Good reputation.One of famous-brand and high-quality freshwater fish that domestic and international market is unanimously approved is turned into.
It is that 1 tail zander raun and 1 tail milter are made into one group, because zander is female when carrying out zander artificial propagation operation Milter difference on morphology is smaller, is almost difficult to accurately distinguish from profile, sometimes obscures female, milter, and finally lead Cause artificial propagation failure.Therefore, how to be effectively to carry out zander large-scale artificial breeding by zander is female, milter makes a distinction Committed step, but prior art does not have related approaches.
The content of the invention
Present invention firstly discloses a kind of zander Sex judging device, using home-made contrivance, antenatal parent population is passed through nephrodinic Hole carries out ovum or sperm collection operation, is ensuring not influence accurately to recognize parent population sex while zander parent is healthy, is grasping Make simple, accurate information is provided for zander artificial propagation.
To achieve the above object of the invention, the present invention is adopted the following technical scheme that:
A kind of zander Sex judging device, including sampling flexible pipe, transition flexible pipe, extraction tube;The extraction tube includes extraction tube sheet Body, pull bar;The extraction tube body one end open;The pull bar is from tube body is extracted in the opening insertion;It is described to push away Pull bar can contact the extraction tube body madial wall and carry out piston movement;The not opening one end of the extraction tube body, which is provided with, to be connect Pipe;The adapter is connected with the extraction tube body;The transition flexible pipe one is terminated with adapter, and one is terminated with sampling flexible pipe.
In above-mentioned technical proposal, the sampling flexible pipe is all inserted into transition flexible pipe with adapter;Sample flexible pipe, transition flexible pipe, Extraction tube formation is flexible coupling, and can play a role power buffering, it is to avoid parent damages to zander.
In above-mentioned technical proposal, the described one end of sampling flexible pipe away from transition flexible pipe is ramp structure, preferably described inclined-plane The inclination angle of structure is 30~70 degree.
In above-mentioned technical proposal, the length of the sampling flexible pipe is 10~15 centimetres, and internal diameter is 1.2~1.6 millimeters.
In above-mentioned technical proposal, the length of the transition flexible pipe is 4~6 centimetres, and internal diameter is 1.8~2.2 millimeters.
In above-mentioned technical proposal, the pull bar can contact the extraction tube body madial wall progress piston movement and refer to Pull bar can be twitched in extraction tube, and twitch during, pull bar outermost wall all the time with extraction tube on the inside of wall seamless Contact, so as to form extraction force in extraction tube, with reference to sampling flexible pipe lateral wall and extraction tube adapter lateral wall all the time with transition Wall seamless contact on the inside of flexible pipe, can take out zander parent's reproduction cell without influence;It is preferred that pull bar includes Rubber end and bar, Rubber end is arranged on bar one end and in extraction tube, so that extraction tube madial wall can be close in pull bar motion process, Gas leakage is avoided to cause sampling to fail.
It is preferred that, one end away from Rubber end is provided with catch on the bar;The cross-sectional area of the catch is more than described take out Take the cross-sectional area of pipe.Catch can be circle, both can more importantly keep sampling process sampling in favor of control pull bar The stabilization of device, especially stability under loading, it is to avoid to zander, parent damages;And avoid pull bar from being absorbed in extraction tube.
In above-mentioned technical proposal, the extraction tube chamber body volume is 5 milliliters;The sampling flexible pipe is provided with scale knot Structure.Groove can be set in sampling flexible pipe lateral wall, represent scale, limit graduated structure, without using conventional coating graduation mark, Both prevented existing graduation mark from producing injury to sample, control that again can be by sampling insertion depth avoids sampling to zander parent Damage, clean operation when being additionally favorable for recycling.
The zander Sex judging device of the present invention can coordinate with cylinder, and cylinder is connected with pull bar, and composition takes automatically Template zander Sex judging device, cylinder control pull bar motion, so as to realize sampling.Therefore, the invention also discloses one kind Automatic sampling type zander Sex judging device, including cylinder and above-mentioned zander Sex judging device;The cylinder connects with pull bar Connect.
The design sample flexible pipe, excessive flexible pipe and extraction tube of the invention, both avoid sampling inner diameter small It effectively can not be connected and extract that bore is small can not provide the defect of suitable extraction force with extraction tube, also solving extraction tube can Can contaminated problem, realize small internal diameter sampling flexible pipe protection zander parent preserve from, large diameter extraction tube draft it is suitable Effect, and three stage structure is reasonable, easy to loading and unloading, also keeps cleaning, and particularly the dismounting of sampling flexible pipe is held after detection every time Easily.
The plastic flexible pipe that the present invention connects terminal slope using extraction tube is acquired ovum or sperm operation, to gather thing It is used as Sex judging standard.Specifically differentiate the method for zander male and female using above-mentioned zander Sex judging device, comprise the following steps:
(1)Before artificial induced spawning, after zander parent is fished for, temporarily support in net cage;
(2)Zander parent is placed in anesthesia liquid and anaesthetized;
(3)Postanesthetic zander parent is placed on workbench desktop, the head and caudal peduncle portion of fish is covered;
(4)Sampling flexible pipe inclined-plane one end of above-mentioned zander Sex judging device is inserted 2~10 centimetres from fish cloacal aperture;
(5)Start pull bar, so that sampling flexible pipe extracts zander parent's reproduction cell, according to zander parent's reproduction cell of extraction Complete zander Sex judging.
In above-mentioned technical proposal, the anesthesia liquid is the anesthesia liquid that every liter of water contains 45ul caryophyllus oils composition;The anesthesia When a length of 5 minutes;The head and caudal peduncle portion of fish are covered with towel.
The invention also discloses application of the above-mentioned zander Sex judging device in zander male and female are differentiated.
A kind of public first zander Sex judging device of the present invention, passes through cloacal aperture to antenatal parent population and carries out ovum or essence Sub- acquisition operations, by the cooperation of structure and size, are ensuring not influence accurately to recognize parent population while zander parent is healthy Sex, accurate information is provided for zander artificial propagation;The beginning of live body zander Sex judging has been started, has been achieved unexpected Technique effect.
Zander Sex judging device disclosed by the invention, if there is the ovum of yellow or yellowish ovum in sampling flexible pipe front end Nest tissue, shows that the tail fish is raun, if front end the sperm of white occurs or do not had whatever, the tail fish is milter;One After tail zander parent's sex identification terminates, plastic tube is cleaned with distilled water, then is placed in 70% alcohol and sterilizes, is then entered The operation of the next tail fish of row;Whole process is simple, reusable edible, particularly will not injure zander, overcome prior art and recognize To take the technology prejudice that reproduction cell can be damaged to zander parent.
Zander Sex judging device disclosed by the invention, transition flexible pipe plays connection and cushioning effect, both ensures extraction tube Extraction force can be efficiently transmitted to sample flexible pipe, take out zander parent so as to effective, avoid causing zander parent again Injury;The convenient sampling flexible pipe insertion cloacal aperture of ramp structure is limited, the damage that operation is caused to fish body is reduced.
Brief description of the drawings
Fig. 1 is the structural representation of the zander Sex judging device of embodiment one;
Fig. 2 is the sampling hose construction schematic diagram of the zander Sex judging device of embodiment one;
Fig. 3 is the pull bar structural representation of the zander Sex judging device of embodiment one;
Wherein, sampling flexible pipe 1, transition flexible pipe 2, extraction tube 3, extraction tube body 4, Rubber end 5, bar 6, ramp structure 7.
Embodiment
Embodiment one
Referring to accompanying drawing 1-3, a kind of zander Sex judging device, including sampling flexible pipe 1, transition flexible pipe 2, extraction tube 3;Extraction tube bag Include extraction tube body 4, pull bar;Extract tube body one end open;Pull bar is from tube body is extracted in opening insertion;Pull bar bag Rubber end 5 and bar 6 are included, Rubber end is arranged on bar one end and in extraction tube, and pull bar can be contacted on the inside of extraction tube body Wall carries out piston movement;The not opening one end of tube body is extracted provided with adapter;Adapter is connected with extracting tube body;Sample flexible pipe with Adapter be all inserted into transition flexible pipe, sampling flexible pipe lateral wall and extraction tube adapter lateral wall all the time with transition flexible pipe inner side wall seamless Contact;The one end of sampling flexible pipe away from transition flexible pipe is the ramp structure 7 that inclination angle is 40 degree;The length for sampling flexible pipe is 12 lis Rice, internal diameter is 1.5 millimeters;The length of transition flexible pipe is 5 centimetres, and internal diameter is 2 millimeters;The cavity volume of extraction tube is 5 milliliters, interior Footpath is more than transition flexible pipe.
Embodiment two
A kind of zander Sex judging device, including sampling flexible pipe, transition flexible pipe, extraction tube;Extraction tube includes extracting tube body, pushed away Pull bar;Extract tube body one end open;Pull bar is from tube body is extracted in opening insertion;Pull bar includes Rubber end, bar, rubber Head is arranged on bar one end and in extraction tube, and pull bar can contact extraction tube body madial wall and carry out piston movement;Extract The not opening one end of tube body is provided with adapter;Adapter is connected with extracting tube body;Sampling flexible pipe is all inserted into transition flexible pipe with adapter In, sampling flexible pipe lateral wall and extraction tube adapter lateral wall are contacted with wall seamless on the inside of transition flexible pipe all the time;Sample flexible pipe remote One end of transition flexible pipe is the ramp structure that inclination angle is 30 degree;The length for sampling flexible pipe is 10 centimetres, and internal diameter is 1.6 millimeters; The length of transition flexible pipe is 6 centimetres, and internal diameter is 2 millimeters;The cavity volume of extraction tube is 5 milliliters, and internal diameter is more than transition flexible pipe.
Embodiment three
A kind of zander Sex judging device, including sampling flexible pipe, transition flexible pipe, extraction tube;Extraction tube includes extracting tube body, pushed away Pull bar;Extract tube body one end open;Pull bar is from tube body is extracted in opening insertion;Pull bar includes Rubber end and bar, rubber Glue head is arranged on bar one end and in extraction tube, and one end away from Rubber end is provided with rounded tabs on bar, and its cross-sectional area is big In the cross-sectional area of extraction tube, pull bar can contact extraction tube body madial wall and carry out piston movement;Tube body is extracted not open One end of mouth is provided with adapter;Adapter is connected with extracting tube body;Sampling flexible pipe is all inserted into transition flexible pipe with adapter, samples flexible pipe Lateral wall and extraction tube adapter lateral wall are contacted with wall seamless on the inside of transition flexible pipe all the time;Sample one of flexible pipe away from transition flexible pipe End is the ramp structure that inclination angle is 70 degree;The length for sampling flexible pipe is 15 centimetres, and internal diameter is 1.2 millimeters;The length of transition flexible pipe Spend for 4 centimetres, internal diameter is 1.8 millimeters;The cavity volume of extraction tube is 5 milliliters, and internal diameter is more than transition flexible pipe;Sample on the outside of flexible pipe Wall is used as graduated structure provided with shallow grooves.
Differentiate the method for zander male and female using the zander Sex judging device of embodiment one, comprise the following steps:
(1)Before artificial induced spawning, after zander parent is fished for, temporarily support in net cage;
(2)Zander parent is placed in the anesthesia liquid that every liter of water contains 45ml caryophyllus oils and anaesthetized, when a length of 5 minutes;
(3)Postanesthetic zander parent is placed on workbench desktop, cloacal aperture periphery is wiped with towel, cover fish head and Caudal peduncle portion;
(4)Sampling flexible pipe inclined-plane one end of above-mentioned zander Sex judging device is inserted 2~10 centimetres from fish cloacal aperture;
(5)Start pull bar, so that sampling flexible pipe extracts zander parent's reproduction cell, the zander parent reproduction cell of extraction is Huang Color is faint yellow, shows that the tail fish is raun;The zander parent reproduction cell of extraction is white or is not drawn into zander parent Reproduction cell, then the tail fish is milter.
The zander Sex judging device of the present invention can coordinate with cylinder, and cylinder is connected with pull bar, and composition takes automatically Template zander Sex judging device, cylinder control pull bar motion, so as to realize automatic sampling, can increase sampling stability. The present invention differentiates zander male and female accuracy rate 100%, and the whole zander cultivation phase detects that none zander is injured, at the same extraction tube without Pollution, sampling flexible pipe can reach the effect recycled by effectively cleaning;Compared with other resolution zander property method for distinguishing, The features such as this method has time saving, laborsaving, reliable and stable;Zander sex identification efficiency, and the degree of accuracy 100% are improved, it is great to answer With value.

Claims (10)

1. a kind of zander Sex judging device, it is characterised in that the zander Sex judging device is soft including sampling flexible pipe, transition Pipe, extraction tube;The extraction tube includes extracting tube body, pull bar;The extraction tube body one end open;The pull bar from The opening insertion is extracted in tube body;The pull bar can contact the extraction tube body madial wall and carry out piston movement; Described not opening one end of tube body of extracting is provided with adapter;The adapter is connected with the extraction tube body;The transition flexible pipe One is terminated with adapter, and one is terminated with sampling flexible pipe.
2. zander Sex judging device according to claim 1, it is characterised in that the sampling flexible pipe was all inserted into adapter Cross in flexible pipe.
3. zander Sex judging device according to claim 1, it is characterised in that the sampling flexible pipe is away from transition flexible pipe One end is ramp structure.
4. zander Sex judging device according to claim 1, it is characterised in that the length of the sampling flexible pipe is 10~15 Centimetre, internal diameter is 1.2~1.6 millimeters.
5. zander Sex judging device according to claim 1, it is characterised in that the length of the transition flexible pipe is 4~6 lis Rice, internal diameter is 1.8~2.2 millimeters.
6. zander Sex judging device according to claim 1, it is characterised in that the pull bar includes Rubber end and bar, Rubber end is arranged on bar one end and in extraction tube.
7. differentiating the method for zander male and female using zander Sex judging device described in claim 1, comprise the following steps:
(1)Before artificial induced spawning, after zander parent is fished for, support in net cage;
(2)Zander parent is placed in anesthesia liquid and anaesthetized;
(3)Postanesthetic zander parent is placed on workbench desktop, the head and caudal peduncle portion of fish is covered;
(4)Sampling flexible pipe inclined-plane one end of zander Sex judging device is inserted 2~10 centimetres from fish cloacal aperture;
(5)Start pull bar, so that sampling flexible pipe extracts zander parent's reproduction cell, according to zander parent's reproduction cell of extraction Complete zander Sex judging.
8. differentiate the method for zander male and female according to claim 7, it is characterised in that the anesthesia liquid is that every liter of water contains The anesthesia liquid of 45ul caryophyllus oils composition;The anesthesia when a length of 5 minutes.
9. differentiate the method for zander male and female according to claim 7, it is characterised in that zander parent's reproduction cell of extraction is Yellow is faint yellow, shows that the tail fish is raun;The zander parent reproduction cell of extraction is white or is not drawn into zander parent This reproduction cell, then the tail fish is milter.
10. application of the zander Sex judging device described in claim 1 in zander male and female are differentiated.
CN201710434158.3A 2017-06-09 2017-06-09 Female and male zander identification device and identification method using same Active CN107047394B (en)

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Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4216782A (en) * 1977-02-15 1980-08-12 Sarstedt W Device for the extraction of blood
JP2000004708A (en) * 1998-06-23 2000-01-11 Kobe Steel Ltd Device for distinguishing male and female of fish
CN202061142U (en) * 2011-04-28 2011-12-07 徐庆锋 Effusion extracting and discharging device for oncology department
CN203029683U (en) * 2013-01-16 2013-07-03 张明娟 Hospital tumour effusion extracting device
CN103430881A (en) * 2013-08-21 2013-12-11 山东省海水养殖研究所 Simple method for rapidly recognizing males and females of salmon and trout juvenile fishes
JP2015126729A (en) * 2013-11-29 2015-07-09 大学共同利用機関法人自然科学研究機構 Sex determination method for germ cell of fish, sex discrimination method, evaluation method for disturbance action of sex differentiation of test substance, hermaphrodite making method, early mature individual making method, germ cell, fish individual, gamete, and cultured cell
CN105287097A (en) * 2015-10-29 2016-02-03 遂宁市中心医院 Silicone oil extracting device for ophthalmology department
CN206866369U (en) * 2017-06-09 2018-01-12 苏州大学 A kind of zander is female, milter identification device

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4216782A (en) * 1977-02-15 1980-08-12 Sarstedt W Device for the extraction of blood
JP2000004708A (en) * 1998-06-23 2000-01-11 Kobe Steel Ltd Device for distinguishing male and female of fish
CN202061142U (en) * 2011-04-28 2011-12-07 徐庆锋 Effusion extracting and discharging device for oncology department
CN203029683U (en) * 2013-01-16 2013-07-03 张明娟 Hospital tumour effusion extracting device
CN103430881A (en) * 2013-08-21 2013-12-11 山东省海水养殖研究所 Simple method for rapidly recognizing males and females of salmon and trout juvenile fishes
JP2015126729A (en) * 2013-11-29 2015-07-09 大学共同利用機関法人自然科学研究機構 Sex determination method for germ cell of fish, sex discrimination method, evaluation method for disturbance action of sex differentiation of test substance, hermaphrodite making method, early mature individual making method, germ cell, fish individual, gamete, and cultured cell
CN105287097A (en) * 2015-10-29 2016-02-03 遂宁市中心医院 Silicone oil extracting device for ophthalmology department
CN206866369U (en) * 2017-06-09 2018-01-12 苏州大学 A kind of zander is female, milter identification device

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