CN104223121B - A kind of extraction element of giant salamander Skin mucus and extracting method - Google Patents
A kind of extraction element of giant salamander Skin mucus and extracting method Download PDFInfo
- Publication number
- CN104223121B CN104223121B CN201410471190.5A CN201410471190A CN104223121B CN 104223121 B CN104223121 B CN 104223121B CN 201410471190 A CN201410471190 A CN 201410471190A CN 104223121 B CN104223121 B CN 104223121B
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- giant salamander
- mucus
- ether
- collection tank
- water
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/04—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from fish or other sea animals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/175—Amino acids
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
- A23L2/66—Proteins
Abstract
The invention discloses a kind of giant salamander Skin mucus extraction element, it is characterised in that：Including collection tank (1) and sealed platform cover (2), the corner angle chamfering that four side walls of collection tank (1) are connected with bottom surface, one angle of sealed platform cover (2) is provided with ether injection orifice (3), and the bottom surface of the collection tank (1) immediately below ether injection orifice (3) is fixed with rayon balls (4).Present invention also offers the method that giant salamander Skin mucus is extracted using the device, is stimulated to giant salamander using ether and is anaesthetized.The method collection mucus is harmless to giant salamander, and ether is volatile, and the internal time of staying is short, and residual is low, is a kind of safely and effectively continuable method for extracting mucus.
Description
Technical field
The present invention relates to a kind of extraction element of giant salamander Skin mucus, the invention further relates to the extraction side of giant salamander Skin mucus
Method, belongs to biogum processing technique field.
Background technology
Giant salamander is commonly called as the giant salamander, is under the jurisdiction of Amphibia Caudata Yin Sai salamanders section, belongs to 3 kinds including 2, i.e. giant salamander category and hidden cheek salamander
Category, wherein giant salamander category again can be divided into Andrias japonicus and Chinese giant salamander, Andrias davidianus, be existing maximum, the most precious amphibian in the world, by
The United Nations lists endangered species protection register in, is national two classes protection aqua-marine life.
As there is giant salamander high nutritive value, medicines and health protection to be worth, especially modern study shows giant salamander Skin mucus
Also there is high Development volue.In recent years, giant salamander artificial propagation and cultivation were increasingly paid attention to by people, with giant salamander people
The ripe and popularization of work raising technology, giant salamander yield are significantly improved, giant salamander two of national departments concerned approved artificial domestication
In generation, can be processed and be sold.
Giant salamander epidermal mucus are a kind of glutinous GL-PPs, belong to a kind of biogum, with resist environmental change ability and
Abundant biologically active.Giant salamander mucus has antibacterial, antioxidation activity, adhesion, has been reported and digests giant salamander epidermal mucus
Into glycopeptide, cosmetics and beverage is developed into.Giant salamander Skin mucus are a kind of renewable resources, and Devoting Major Efforts To Developing is viscous using giant salamander
Liquid, will drive the development of giant salamander culture industry and promote wildlife conservation.
How non-damaging, continuous and effective collect enough giant salamander mucus raw materials, are crucial rings of mucus intensive processing
Section.In terms of giant salamander mucus is collected, existing method is electric shocking method and scrapes back-packing.Electric shocking method does not produce mucus at lower voltages, high
Under voltage, IR is larger, affects giant salamander physical efficiency recovery；And the mucus for scraping back-packing acquisition usually has pollution of casting off a skin, mucus is produced
Less, do not produce mucus sometimes, scraping easily makes dermatorrhagia and produces damage, and giant salamander is upset
Operating personnel.Therefore, there is easily pollution, measure less, giant salamander is had in existing mucus acquisition method.
Content of the invention
For existing problem, it is an object of the invention to provide a kind of extraction element of giant salamander Skin mucus, the present invention
The extracting method of safe giant salamander Skin mucus is additionally provided, the method causes giant salamander physical efficiency recovery fast, not damaged.
To achieve these goals, the technical scheme is that：A kind of giant salamander Skin mucus extraction element, its feature exist
In：Including collection tank and sealed platform cover, the corner angle chamfering that four side walls of the collection tank are connected with bottom surface, the sealing
One angle of case lid is provided with ether injection orifice, and the bottom surface of the collection tank immediately below the injection orifice is fixed with rayon balls.
In above-mentioned technical proposal：The sealed platform cover transparent material is made.
In above-mentioned technical proposal：The collection tank is made up of Stainless Steel or plastics.
It is another object of the present invention to be achieved in that：A kind of extracting method of giant salamander Skin mucus, it is characterised in that：Bag
Include following steps：
(a), temporarily support：The fresh and alive giant salamander for having stopped eating is put into and is filled the storage pond, holding pond of fresh cold water and is temporarily supported, water level requires energy
Whole submergence giant salamander backs, water temperature are less than 20 DEG C, temporarily support time >=4 hour, and water keeps flowing and cleaning；
(b), cleaning：Temporarily support after terminating, change clear water, giant salamander body surface is gently scrubbed with sponge brush, to clean impurity and slough off
Skin, goes water to drain；
(c), the giant salamander after cleaning is placed in the extraction element described in claim 1, the head of giant salamander is towards degreasing
Cotton balls；
(d), stimulate and anaesthetize：With syringe draw ether, from ether injection orifice to collection device in inject ether, ether
On rayon balls immediately below being dropped in, ether injection orifice is sealed, is upset after giant salamander suction ether, body surface produces viscous
Liquid, while giant salamander is by etherization；
E (), mucus are collected and are preserved：The white slimy that giant salamander body surface is gently scraped with spoon, the mucus of collection is placed in
Crisper, pre-freeze, vacuum freeze drying, normal temperature are preserved.
In above-mentioned technical proposal：In step (d), the amount of ether is 5-100ml, and giant salamander reaches anesthesia shape in 30min
State.The amount of such ether can cause giant salamander to produce more mucus.
In above-mentioned technical proposal：In step (e), pre-freezing temperature is -10 DEG C～-20 DEG C.
Beneficial effect：Compared with prior art, the present invention has the benefit that：
1. the method collection mucus is harmless to giant salamander, and ether is volatile, and the internal time of staying is short, and residual is low, giant salamander is received
Stimulate to ether and both produced mucus, be anesthetized again, it is to avoid the attack to operating personnel, be a kind of side of safe extraction mucus
Method；
2. the extraction element of the present invention is easy to control ether consumption, and sealed platform cover is anaesthetized using transparent material observable giant salamander
Process.
3. the extraction element of the present invention reduces impact of the ether gas to operating personnel.The extraction element of the present invention is by side wall
Connect corner angle chamfering with bottom surface, be so easy to the scraping for sticking to the mucus on collection tank angle.
4. the extracting method of the present invention can collect a large amount of mucus, and it is fresh that a general adult giant salamander can produce 20-40g
Mucus, after collection mucus, the stimulated little, not damaged of giant salamander recovers fast, and cultivation a period of time can carry out mucus collection again, be one
Plant safely and effectively sustainable acquisition method.
5. the method for the present invention, easy to operate, efficiency high, is suitable to large-scale production application.
Description of the drawings
Fig. 1 is the structural representation of the extraction element of the present invention.
Specific embodiment
The present invention will be further described with specific embodiment below in conjunction with the accompanying drawings：
Embodiment 1
The giant salamander Skin mucus extraction element of the present invention is by collection tank 1, sealed platform cover 2, ether injection orifice 3, absorbent cotton
4 grade part of ball is constituted.
Collection tank 1 is the square box being made up of stainless steel or plastics, and a length of 50-70cm of collection tank 1 is a width of
15-30cm, a height of 20cm.The corner angle chamfering that four side walls of collection tank 1 are connected with bottom surface, four side walls of collection tank 1
Excessively it is connected with bottom surface circular arc.The scraping of the mucus being so easy in collection tank 1.The top of collection tank 1 is provided with can open
The sealed platform cover 2 being made of clear material, sealed platform cover 2 can be with 1 tight closure of collection tank.The one of the sealed platform cover 2
Individual angle is provided with the ether injection orifice 3 of 5mm or so, and the bottom surface of the collection tank 1 immediately below the injection orifice 3 is fixed with diameter
The rayon balls 4 of 3cm or so.
The extracting method of the giant salamander Skin mucus of the present invention, is completed in accordance with the following steps：
(a), temporarily support：The fresh and alive giant salamander for having stopped eating is put into and is filled the storage pond, holding pond of fresh cold water and is temporarily supported, water level requires energy
Whole submergence giant salamander backs, water temperature are less than 20 DEG C, temporarily support time >=4 hour, and the flow velocity that water keeps certain slowly flows, and water is also needed
Keep cleaning.
(b), cleaning：Temporarily support after terminating, change clear water, giant salamander body surface is gently scrubbed with sponge brush, to clean impurity and slough off
Skin, goes water to drain.
(c), the giant salamander after cleaning is placed in said extracted device, the head of giant salamander is towards rayon balls.
(d), stimulate and anaesthetize：Collection device is placed in ventilated environment operation, ether is drawn with syringe, inject from ether
5-100ml ether is injected in hole 3 into collection device, ether be dropped in immediately below rayon balls 4 on, take out syringe, with de-
Fat cotton blocks ether injection orifice 3, reaches narcosis after giant salamander suction ether in 30min, and giant salamander body surface produces mucus.
E (), mucus are collected and are preserved：The white slimy that giant salamander body surface is gently scraped with stainless steel spoon, viscous by collected
Liquid is placed in crisper, and -20 DEG C or so pre-freezes, vacuum freeze drying, normal temperature preserve.
F () is adopted rear giant salamander and is recovered：Rear giant salamander will be adopted and be transferred to the storage pond, holding pond containing little water, water should not cover the giant salamander back of the body
Portion, it is to avoid choke giant salamander, removes the mucus for remaining in body surface, and giant salamander recovers revival in about 1 hour or so, continues according to it after revival
Front method for breeding breeding observing.
The preferred embodiment of the present invention described in detail above.It should be appreciated that one of ordinary skill in the art without
Need creative work just can make many modifications and variations according to the design of the present invention.Therefore, all technology in the art
Personnel are available by logical analysis, reasoning, or a limited experiment on the basis of existing technology under this invention's idea
Technical scheme, all should be in the protection domain being defined in the patent claims.
Claims (2)
1. a kind of extracting method of giant salamander Skin mucus, it is characterised in that：Comprise the steps：
(a), temporarily support：The fresh and alive adult giant salamander for having stopped eating is put into and is filled the storage pond, holding pond of fresh cold water and is temporarily supported, water level requires energy
Whole submergence giant salamander backs, water temperature are less than 20 DEG C, temporarily support time >=4 hour, and water keeps flowing and cleaning；
(b), cleaning：Temporarily support after terminating, change clear water, giant salamander body surface is gently scrubbed with sponge brush, to clean impurity and cast off a skin, go
Water is drained；
(c), the giant salamander after cleaning is placed in extraction element, extraction element, including collection tank (1) and sealed platform cover (2),
Collection tank (1) is made up of Stainless Steel or plastics；
Sealed platform cover (2) are made of clear material, and the corner angle that four side walls of collection tank (1) are connected with bottom surface fall
Angle, an angle of sealed platform cover (2) are provided with ether injection orifice (3), the collecting box immediately below ether injection orifice (3)
The bottom surface of body (1) is fixed with rayon balls (4)；The head of giant salamander is towards rayon balls；
(d), stimulate and anaesthetize：Ether is drawn with syringe, and 5-100ml second is injected into collection device from ether injection orifice (3)
Ether, ether are dropped on the rayon balls (4) of underface, seal ether injection orifice (3), and giant salamander suction ether is upset, greatly
Salamander reaches narcosis in 30min, and giant salamander body surface produces mucus, while giant salamander is anesthetized；
E (), mucus are collected and are preserved：The white slimy that giant salamander body surface is gently scraped with spoon, the mucus of collection is placed in fresh-keeping
Box, pre-freeze, vacuum freeze drying, normal temperature are preserved.
2. the extracting method of giant salamander Skin mucus according to claim 1, it is characterised in that：In step (e) pre-freezing temperature for-
10 DEG C～-20 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410471190.5A CN104223121B (en) | 2014-09-16 | 2014-09-16 | A kind of extraction element of giant salamander Skin mucus and extracting method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410471190.5A CN104223121B (en) | 2014-09-16 | 2014-09-16 | A kind of extraction element of giant salamander Skin mucus and extracting method |
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| CN104223121A CN104223121A (en) | 2014-12-24 |
| CN104223121B true CN104223121B (en) | 2017-03-15 |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105494257B (en) * | 2015-12-29 | 2018-05-22 | 贵州大学 | The method for promoting the method for giant salamander husking and extracting giant salamander genome |
| CN105815425B (en) * | 2016-04-28 | 2017-11-14 | 陕西理工学院 | Giant salamander slaughters machine |
| CN106614137B (en) * | 2016-12-19 | 2019-07-16 | 界首市鑫一龙机械设备购销有限公司 | A kind of collection method of swamp eel mucus |
| CN106619739B (en) * | 2016-12-28 | 2020-06-30 | 安顺市四海农业科技开发有限公司 | Artificial collection method of giant salamander surface mucus |
| CN108634235B (en) * | 2018-07-13 | 2024-02-09 | 贵阳学院 | Giant salamander mucus collection device |
| CN109601437B (en) * | 2019-01-25 | 2021-06-15 | 淮海工学院 | Method for stress secretion and collection of loach mucus |
| CN111933093A (en) * | 2020-08-21 | 2020-11-13 | 张家界鲵泰大鲵养殖专业合作社 | Method for preparing musical instrument skin by using artificially-cultured second-generation giant salamander skin and musical instrument skin |
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| CN102764273A (en) * | 2012-08-14 | 2012-11-07 | 新疆师范大学 | Method for purifying skin secretions of tailed amphibian ranodon sibiricus and application |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1336383A (en) * | 2000-07-29 | 2002-02-20 | 中国科学院昆明动物研究所 | Giant web bombinin and its prepn. and pharmaceutical application |
| CN102335288A (en) * | 2010-07-20 | 2012-02-01 | 陕西理工学院 | Giant salamander skin mucus scalding ointment |
| US10617790B2 (en) * | 2013-01-09 | 2020-04-14 | Ise Professional Testing & Consulting Services, Inc. | Decellularized biomaterial from non-mammalian tissue |
| CN103829235B (en) * | 2014-01-11 | 2015-07-01 | 陕西理工学院 | Preparation method of giant salamander skin mucus whole powder |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102764273A (en) * | 2012-08-14 | 2012-11-07 | 新疆师范大学 | Method for purifying skin secretions of tailed amphibian ranodon sibiricus and application |
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