CN106619739B - Artificial collection method of giant salamander surface mucus - Google Patents
Artificial collection method of giant salamander surface mucus Download PDFInfo
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- CN106619739B CN106619739B CN201611231165.5A CN201611231165A CN106619739B CN 106619739 B CN106619739 B CN 106619739B CN 201611231165 A CN201611231165 A CN 201611231165A CN 106619739 B CN106619739 B CN 106619739B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/65—Amphibians, e.g. toads, frogs, salamanders or newts
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/0005—Ingredients of undetermined constitution or reaction products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/001—Use of materials characterised by their function or physical properties
- A61L24/0015—Medicaments; Biocides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/30—Compounds of undetermined constitution extracted from natural sources, e.g. Aloe Vera
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/418—Agents promoting blood coagulation, blood-clotting agents, embolising agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/04—Materials for stopping bleeding
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Abstract
An artificial collection method of giant salamander surface mucus relates to the field of cultivation, and comprises the following steps: a. selecting giant salamanders; b. sterilizing the body surface; c. artificial electrification is carried out; d. waiting; e. collecting mucus; f. and (6) sterilizing. The invention manually collects the surface mucus of the giant salamander, utilizes the second-level hemostasis and bonding function of the giant salamander mucus, and has extremely wide prospect in the fields of medical surgery and the like because the giant salamander mucus is a pure natural biological raw material and has no toxic or side effect. By collecting and utilizing the mucus, the body surface mucus can be used as a raw material of a high-quality medical adhesive and can also be used for other medical purposes.
Description
Technical Field
The invention relates to the field of cultivation, in particular to a method for manually collecting mucus on the surface of a giant salamander.
Background
Giant salamander (giant salamander) as a famous giant salamanderThe name of Latin is Andrias davidianus) The amphibian is the largest and most precious amphibian existing in the world. It sounds much like a baby's cry, so people also call it "giant salamander". Giant salamander is an animal with high edible value and a traditional famous and precious medicinal animal, and muscles, internal organs, bones, epidermis and secretions of the giant salamander can be used as medicines.
The traditional proved recipe of the giant salamander skin powder mixed with the tung oil can treat burns and scalds, and particularly, scars can not be left in the treatment of the burns and the scalds of the face. After release of mucinous proteins in the mucus cells of the giant salamander skin, the mucus is combined with water to form mucus, and the mucus covers the free surface of the upper skin to form a natural protective barrier. The mucus contains mainly mucopolysaccharide protein and fibrous substance, and also contains various hormones, and bombesin, and has strong antibacterial effect, and can be used for preventing leprosy and senile dementia and preventing and treating cancer.
In modern medical field, many diseases require surgical excision of lesions, surgical injuries can form wounds on the surface of human body, and the rapid healing of the wounds can reduce the injuries of the human body caused by operations or traumas, and the traditional suture type is gradually replaced by adhesives. The common adhesives in the domestic medical industry are fibrin (also called fibrin) and cyanoacrylate. Fibrin has weak adhesiveness due to the action mechanism, and is very important for nursing patients after use, but can cause wound rupture if being careless, and can cause secondary injury of the wound if being serious, while cyanoacrylate is used as a chemical adhesive, although the adhesive quality is far better than that of fibrin, the cyanoacrylate has large toxic and side effects, and is not suitable for people with slight physical sensitivity.
Modern researches find that the mucus on the body surface of the giant salamander has extremely strong adhesiveness and second-level hemostasis adhesion, and the giant salamander mucus is a pure natural biological raw material and has no toxic or side effect, so the giant salamander mucus has extremely wide prospect in the fields of medical surgery and the like. Because the giant salamander is in a harsh living environment and cannot be cultured in a large-scale and generalized manner, the method for obtaining the giant salamander body surface mucus is very traditional, the mucus on the skin surface layer of the giant salamander can be collected after the giant salamander is killed, and the application of the giant salamander mucus in medical adhesives is limited due to the rare of the giant salamander mucus. Therefore, it is very important to find a large-scale normalized collection method for the surface mucus of the giant salamander.
Disclosure of Invention
The invention provides a method for manually collecting surface mucus of a giant salamander, which aims to solve the technical problems that the surface mucus of the giant salamander can only be collected through the killed giant salamander, the collected mucus is small in amount and has no sustainability in the prior art.
A method for manually collecting mucus on the surface of a giant salamander comprises the following steps:
a. selecting giant salamanders: selecting giant salamanders which are more than three years and a half years old, are not frightened within 25 days and are not mucus within 25 days;
b. body surface disinfection: manually cleaning the body surface of the giant salamander with light saline water to clean the skin surface of the giant salamander;
c. artificial electrification: passing low voltage below 36V to the surface of the giant salamander;
d. waiting: standing for 50 seconds, and naturally secreting and accumulating mucus on the surface of the giant salamander;
e. collecting mucus: collecting mucus on the surface of the giant salamander, slowly increasing the force, but taking the principle of not damaging the surface tissue of the giant salamander as a principle;
f. and (3) disinfection: and sterilizing the body surface of the giant salamander after the mucus is taken, and then putting the giant salamander back to the culture pond.
Further, in the step b, the concentration of the dilute saline used is 2-3%, if the concentration is too low, the sterilization effect is affected, and if the concentration of the dilute saline is high, the medicinal effect of the collected mucus is affected.
Furthermore, the voltage value in the step c is 30-36V, and the effect is optimal.
Further, in the step c, the current is 0.05-0.1 milliampere hour, and the duration is 3-6 seconds. If the concentration is lower than the data, the collection amount of mucus is small, and the economic benefit is influenced; if the number exceeds the above value, invisible damage can be formed on the giant salamander body, the giant salamander after collecting mucus is anorexia and easy to be frightened, and the body surface trauma is easy to be infected within 15 days after the mucus is taken. The above values are therefore considered in several respects: 1. the maximum amount of mucus collected, 2, does not affect the normal growth of the giant salamander, 3, the chance of traumatic infection at a month after mucus collection cannot be increased. And after the statistical data of three hundred giant salamanders in three years prove, the data can completely collect the maximum mucus secreted by the body surface of the giant salamanders on the basis of normal growth of the giant salamanders, and after the data is combined with the step f, the collected mucus can not influence the growth of the giant salamanders at all, the wound infection in one month after the collection and the quantity of the mucus collected by the giant salamanders in the next month (the statistical data is the maximum value of the quantity collected by the giant salamanders in three years).
And f, after collecting mucus, sterilizing the mucus by using gentian violet liquid with the concentration of less than 1.5 percent before breeding again, and spraying or coating the mucus on the body surface of the giant salamander.
The invention manually collects the surface mucus of the giant salamander, utilizes the second-level hemostasis and bonding function of the giant salamander mucus, and has extremely wide prospect in the fields of medical surgery and the like because the giant salamander mucus is a pure natural biological raw material and has no toxic or side effect. By collecting and utilizing the mucus, the body surface mucus can be used as a raw material of a high-quality medical adhesive and can also be used for other medical purposes.
Detailed Description
In order to more fully explain the practice of the present invention, the following examples are provided to further illustrate the technical effects of the present invention. The examples are given solely for the purpose of illustration and are not intended to limit the scope of the invention.
Selecting giant salamanders cultured in the same batch, dividing the giant salamanders into two batches (20 giant salamanders selected by the applicant in total), wherein mucus is collected in one batch, mucus is not collected in the other batch, the giant salamanders are of two ages, and the weight is as follows: 2.5 kg plus or minus 50 g, the test results are completed with the following parameters: manually cleaning the body surface of the giant salamander with light saline water to clean the skin surface of the giant salamander; the low voltage of 36V is applied to the surface of the giant salamander, the current is 0.06 milliampere hour, and the duration is 5 seconds; standing for 50 seconds, and naturally secreting and accumulating mucus on the surface of the giant salamander; collecting mucus on the surface of the giant salamander, slowly increasing the force, but taking the principle of not damaging the surface tissue of the giant salamander as a principle; and sterilizing the body surface of the giant salamander after taking mucus by using gentian violet liquid with the concentration of 1%, and then putting the giant salamander into the culture pond after sterilizing.
By adopting the method, every month, 0.4-0.6kg of mucus on the surface of the giant salamander can be collected. Statistical data, and the comparison of the body surface mucus collection time for one year shows that the body weight of the giant salamander which always collects the body surface mucus is basically equal to that of the giant salamander which does not collect the body surface mucus, so that the body surface mucus collection has no influence on the giant salamander.
The above description is only an example of the present invention and should not be taken as limiting the scope of the present invention, and all equivalent, changes and modifications made by the contents described in the present application are intended to be included within the scope of the present invention.
Claims (5)
1. A method for manually collecting mucus on the surface of a giant salamander comprises the following steps:
a. selecting giant salamanders: selecting giant salamanders which are more than three years and a half years old, are not frightened within 25 days and are not mucus within 25 days;
b. body surface disinfection: manually cleaning the body surface of the giant salamander with light saline water to clean the skin surface of the giant salamander;
c. artificial electrification: passing low voltage below 36V to the surface of the giant salamander;
d. waiting: standing for 50 seconds, and naturally secreting and accumulating mucus on the surface of the giant salamander;
e. collecting mucus: collecting mucus on the surface of the giant salamander, slowly increasing the force, but taking the principle of not damaging the surface tissue of the giant salamander as a principle;
f. and (3) disinfection: and sterilizing the body surface of the giant salamander after the mucus is taken, and then putting the giant salamander back to the culture pond.
2. The method of claim 1, wherein the step of manually collecting mucus on the surface of the giant salamander comprises: in the step b, the concentration of the dilute brine is 2-3%.
3. The method of claim 1, wherein the step of manually collecting mucus on the surface of the giant salamander comprises: the voltage value in the step c is 30-36V, and the effect is optimal.
4. The method of claim 1, wherein the step of manually collecting mucus on the surface of the giant salamander comprises: and f, sterilizing with gentian violet liquid with concentration below 1.5% before re-stocking, and spraying or coating on the surface of the giant salamander.
5. The method for manually collecting mucus on the surface of a giant salamander according to any one of claims 1-4, wherein: in the step c, the current is 0.05-0.1 milliampere hour, and the duration is 3-6 seconds.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611231165.5A CN106619739B (en) | 2016-12-28 | 2016-12-28 | Artificial collection method of giant salamander surface mucus |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611231165.5A CN106619739B (en) | 2016-12-28 | 2016-12-28 | Artificial collection method of giant salamander surface mucus |
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| Publication Number | Publication Date |
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| CN106619739A CN106619739A (en) | 2017-05-10 |
| CN106619739B true CN106619739B (en) | 2020-06-30 |
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| CN109601437B (en) * | 2019-01-25 | 2021-06-15 | 淮海工学院 | Method for stress secretion and collection of loach mucus |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104223121A (en) * | 2014-09-16 | 2014-12-24 | 重庆馗旭生物科技股份有限公司 | Extracting device for giant salamander skin mucus and extracting method |
| CN104542454A (en) * | 2014-10-24 | 2015-04-29 | 重庆翰锋农业发展有限公司 | Giant salamanders artificial breeding technology |
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- 2016-12-28 CN CN201611231165.5A patent/CN106619739B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104223121A (en) * | 2014-09-16 | 2014-12-24 | 重庆馗旭生物科技股份有限公司 | Extracting device for giant salamander skin mucus and extracting method |
| CN104542454A (en) * | 2014-10-24 | 2015-04-29 | 重庆翰锋农业发展有限公司 | Giant salamanders artificial breeding technology |
Non-Patent Citations (1)
| Title |
|---|
| 成年大鲵实验室饲养及蜕皮和体表粘液收集方法;许永杰等;《遵义医学院学报》;20140228;第37卷(第1期);第115-118页 * |
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Effective date of registration: 20221209 Address after: 277700 Second floor of New Times Building, Lanling County Economic Development Zone, Linyi City, Shandong Province Patentee after: Lanling Finance Industry Development Co.,Ltd. Address before: 561208 Shuitangzhai, Chengguan Town, Zhenning Buyi Miao Autonomous County, Anshun City, Guizhou Province Patentee before: Anshun Sihai Agricultural Science & Technology Development Co.,Ltd. |
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