CN104223121A - Extracting device for giant salamander skin mucus and extracting method - Google Patents
Extracting device for giant salamander skin mucus and extracting method Download PDFInfo
- Publication number
- CN104223121A CN104223121A CN201410471190.5A CN201410471190A CN104223121A CN 104223121 A CN104223121 A CN 104223121A CN 201410471190 A CN201410471190 A CN 201410471190A CN 104223121 A CN104223121 A CN 104223121A
- Authority
- CN
- China
- Prior art keywords
- giant salamander
- mucus
- ether
- extracting
- extracting method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/04—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from fish or other sea animals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/175—Amino acids
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
- A23L2/66—Proteins
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Mycology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Extraction Or Liquid Replacement (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses an extracting device for giant salamander skin mucus. The extracting device is characterized by comprising a collecting tank (1) and a sealing tank cover (2), wherein corn angles formed by connecting four side walls and the bottom surface of the collecting tank (1) are chamfered, one corner of the sealing tank cover (2) has a diethyl ether injection hole (3), an absorbent ball (4) is fixed at the bottom surface of the collecting tank (1) right below the diethyl ether injection hole (3). The invention further provides an extracting method for extracting giant salamander skin mucus by using the device, wherein the giant salamander is stimulated and narcotized by using diethyl ether. The extracting method is harmless to the giant salamander when being used for collecting mucus, diethyl ether easily volatilizes, stains in the body for a short time, and is little in residue, therefore, the extracting method is safe, effective, and is capable of continuously extracting mucus.
Description
Technical field
The present invention relates to a kind of extraction element of giant salamander Skin mucus, the invention still further relates to the extracting method of giant salamander Skin mucus, belong to biogum processing technique field.
Background technology
Giant salamander is commonly called as the giant salamander; be under the jurisdiction of Amphibia Caudata Yin Sai salamander section; comprise 2 genus 3 kinds; namely giant salamander belongs to and hidden cheek salamander genus; wherein giant salamander belongs to and can be divided into Andrias japonicus and Chinese giant salamander, Andrias davidianus again; be existing maximum, the most precious amphibian in the world, united state lists endangered species protection register in, is national two class protection aqua-marine lifes.
Because giant salamander has high nutritive value, medicines and health protection value, especially modern study shows that giant salamander Skin mucus also has high Development volue.In recent years, giant salamander artificial propagation and cultivation were more and more subject to people and paid attention to, and along with maturation and the popularization of giant salamander artificial breeding technology, giant salamander output significantly improves, and the giant salamander second filial of national departments concerned approved artificial domestication can be processed and sell.
Giant salamander epidermal mucus is a kind of glutinous GL-PP, belongs to a kind of biogum, has the ability and abundant biologically active of resisting environmental change.Giant salamander mucus has antibacterial, antioxidation activity, adhesion, has been reported and giant salamander epidermal mucus enzymolysis is become glycopeptide, develop into cosmetics and beverage.Giant salamander Skin mucus is a kind of renewable resource, and Devoting Major Efforts To Developing utilizes giant salamander mucus, giant salamander culture industry will be driven to develop and promote wildlife conservation.
How non-damaging, continuous and effective collects enough giant salamander mucus raw materials, is a key link of mucus intensive processing.In collection giant salamander mucus, existing method is electric shocking method and scrapes back-packing.Electric shocking method does not produce mucus at lower voltages, and under high voltage, IR is comparatively large, affects giant salamander physical efficiency recovery; And scrape the mucus that back-packing obtains and usually have pollutions of casting off a skin, produce mucus less, sometimes do not produce mucus, swiping easily makes dermatorrhagia and produces damage, and giant salamander is upset easy attack operation personnel.Therefore, existing mucus acquisition method existence easily pollution, amount have the problems such as damage less, to giant salamander.
Summary of the invention
For existing problem, the object of the present invention is to provide a kind of extraction element of giant salamander Skin mucus, present invention also offers the extracting method of safe giant salamander Skin mucus, the method makes giant salamander physical efficiency recovery fast, not damaged.
To achieve these goals, technical scheme of the present invention is: a kind of giant salamander Skin mucus extraction element, it is characterized in that: comprise collection tank and sealed platform cover, the corner angle chamfering that four sidewalls of described collection tank are connected with bottom surface, an angle of described sealed platform cover is provided with ether injection orifice, and the bottom surface of the collection tank immediately below described injection orifice is fixed with rayon balls.
In technique scheme: described sealed platform cover transparent material is made.
In technique scheme: described collection tank is made up of Stainless Steel or plastics.
Another object of the present invention is achieved in that a kind of extracting method of giant salamander Skin mucus, it is characterized in that: comprise the steps:
(a), support temporarily: the fresh and alive giant salamander stopped eating is put into the storage pond, holding pond filling fresh cold water and supports temporarily, water level requires can whole submergence giant salamander backs, and water temperature is lower than 20 DEG C, and the time of supporting temporarily >=4 hours, water keeps flowing and clean;
B (), cleaning: support temporarily after terminating, change clear water, scrub giant salamander body surface gently with sponge brush, with clean impurity with cast off a skin, anhydrate and drain;
(c), by clean after giant salamander be placed in extraction element according to claim 1, the head of giant salamander is towards rayon balls;
(d), stimulation and anesthesia: draw ether with syringe, in gathering-device, inject ether from ether injection orifice, on the rayon balls immediately below ether is dropped in, sealing ether injection orifice, giant salamander is upset after sucking ether, and namely body surface produces mucus, and giant salamander is by etherization simultaneously;
E (), mucus are collected and are preserved: with the white slimy of spoon scraping giant salamander body surface gently, the mucus of collection is placed in crisper, pre-freeze, vacuum freeze drying, normal temperature is preserved.
In technique scheme: in step (d), the amount of ether is 5-100ml, and giant salamander reaches narcosis in 30min.The amount of such ether can make giant salamander produce more mucus.
In technique scheme: in step (e), pre-freezing temperature is-10 DEG C ~-20 DEG C.
Beneficial effect: compared with prior art, the present invention has following benefit:
1. the method collection mucus is harmless to giant salamander, and ether is volatile, and in body, the time of staying is short, residual low, giant salamander is subject to ether stimulation and both produced mucus, and being anaesthetized again, avoid the attack to operating personnel, is a kind of method of safe extraction mucus;
2. extraction element of the present invention is convenient to control ether consumption, and sealed platform cover adopts transparent material observable giant salamander anaesthesia process.
3. extraction element of the present invention reduces ether gas to the impact of operating personnel.Sidewall is connected corner angle chamfering with bottom surface by extraction element of the present invention, is convenient to the scraping of the mucus sticked on collection tank angle like this.
4. extracting method of the present invention can collect a large amount of mucus, and a general adult giant salamander can produce the fresh mucus of 20-40g, after gathering mucus, giant salamander irriate is little, not damaged, recover fast, cultivation a period of time can carry out mucus collection again, is one sustainable acquisition method safely and effectively.
5. method of the present invention, easy and simple to handle, and efficiency is high, is suitable for large-scale production application.
Accompanying drawing explanation
Fig. 1 is the structural representation of extraction element of the present invention.
Detailed description of the invention
Below in conjunction with the drawings and specific embodiments, the present invention will be further described:
Embodiment 1
Giant salamander Skin mucus extraction element of the present invention is made up of parts such as collection tank 1, sealed platform cover 2, ether injection orifice 3, rayon balls 4.
Collection tank 1 is the square box be made up of stainless steel or plastics, and the length of collection tank 1 is 50-70cm, and wide is 15-30cm, and height is 20cm.The corner angle chamfering that four sidewalls of collection tank 1 are connected with bottom surface, four sidewalls of collection tank 1 are excessively connected with bottom surface circular arc.Be convenient to the scraping of the mucus in collection tank 1 like this.The top of collection tank 1 is provided with the openable sealed platform cover 2 be made up of transparent material, sealed platform cover 2 can with collection tank 1 tight closure.An angle of described sealed platform cover 2 is provided with the ether injection orifice 3 of about 5mm, and the bottom surface of the collection tank 1 immediately below described injection orifice 3 is fixed with the rayon balls 4 of diameter about 3cm.
The extracting method of giant salamander Skin mucus of the present invention, completes in accordance with the following steps:
(a), support temporarily: the fresh and alive giant salamander stopped eating is put into the storage pond, holding pond filling fresh cold water and supports temporarily, water level requires can whole submergence giant salamander backs, water temperature lower than 20 DEG C, the time of supporting temporarily >=4 hours, water keeps certain flow velocity slowly to flow, and water also needs to keep clean.
B (), cleaning: support temporarily after terminating, change clear water, scrub giant salamander body surface gently with sponge brush, with clean impurity with cast off a skin, anhydrate and drain.
(c), the giant salamander after cleaning is placed in said extracted device, the head of giant salamander is towards rayon balls.
(d), stimulation and anesthesia: gathering-device is placed in ventilated environment operation, ether is drawn with syringe, in gathering-device, 5-100ml ether is injected from ether injection orifice 3, on rayon balls 4 immediately below ether is dropped in, take out syringe, block ether injection orifice 3 with absorbent cotton, reach narcosis in 30min after giant salamander sucks ether, namely giant salamander body surface produces mucus.
E (), mucus are collected and are preserved: with the white slimy of stainless steel spoon scraping giant salamander body surface gently, the mucus of collection is placed in crisper, about-20 DEG C pre-freezes, vacuum freeze drying, normal temperature is preserved.
F () is adopted rear giant salamander and is recovered: be transferred to storage pond, holding pond containing little water by adopting rear giant salamander, water should not cover giant salamander back, avoids choking giant salamander, and removing remains in the mucus of body surface, giant salamander about 1 hours recovers to revive, and continues according to method for breeding breeding observing before after reviving.
More than describe preferred embodiment of the present invention in detail.Should be appreciated that those of ordinary skill in the art just design according to the present invention can make many modifications and variations without the need to creative work.Therefore, all technical staff in the art, all should by the determined protection domain of claims under this invention's idea on the basis of existing technology by the available technical scheme of logical analysis, reasoning, or a limited experiment.
Claims (6)
1. a giant salamander Skin mucus extraction element, it is characterized in that: comprise collection tank (1) and sealed platform cover (2), the corner angle chamfering that four sidewalls of described collection tank (1) are connected with bottom surface, an angle of described sealed platform cover (2) is provided with ether injection orifice (3), and the bottom surface of the collection tank (1) immediately below described ether injection orifice (3) is fixed with rayon balls (4).
2. giant salamander Skin mucus extraction element according to claim 1, is characterized in that: described sealed platform cover (2) is made up of transparent material.
3. giant salamander Skin mucus extraction element according to claim 1, is characterized in that: described collection tank (1) is made up of Stainless Steel or plastics.
4. an extracting method for giant salamander Skin mucus, is characterized in that: comprise the steps:
(a), support temporarily: the fresh and alive adult giant salamander stopped eating is put into the storage pond, holding pond filling fresh cold water and supports temporarily, water level requires can whole submergence giant salamander backs, and water temperature is lower than 20 DEG C, and the time of supporting temporarily >=4 hours, water keeps flowing and clean;
B (), cleaning: support temporarily after terminating, change clear water, scrub giant salamander body surface gently with sponge brush, with clean impurity with cast off a skin, anhydrate and drain;
(c), by clean after giant salamander be placed in extraction element according to claim 1, the head of giant salamander is towards rayon balls;
(d), stimulation and anesthesia: draw ether with syringe, in gathering-device, ether is injected from ether injection orifice (3), on rayon balls (4) immediately below ether is dropped in, sealing ether injection orifice (3), giant salamander sucks ether and is upset, namely giant salamander body surface produces mucus, and giant salamander is anaesthetized simultaneously;
E (), mucus are collected and are preserved: with the white slimy of spoon scraping giant salamander body surface gently, the mucus of collection is placed in crisper, pre-freeze, vacuum freeze drying, normal temperature is preserved.
5. the extracting method of giant salamander Skin mucus according to claim 4, it is characterized in that: in step (d), the amount of ether is 5-100ml, and giant salamander reaches narcosis in 30min.
6. the extracting method of giant salamander Skin mucus according to claim 4, is characterized in that: in step (e), pre-freezing temperature is-10 DEG C ~-20 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410471190.5A CN104223121B (en) | 2014-09-16 | 2014-09-16 | A kind of extraction element of giant salamander Skin mucus and extracting method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410471190.5A CN104223121B (en) | 2014-09-16 | 2014-09-16 | A kind of extraction element of giant salamander Skin mucus and extracting method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104223121A true CN104223121A (en) | 2014-12-24 |
| CN104223121B CN104223121B (en) | 2017-03-15 |
Family
ID=52212728
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410471190.5A Active CN104223121B (en) | 2014-09-16 | 2014-09-16 | A kind of extraction element of giant salamander Skin mucus and extracting method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104223121B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105494257A (en) * | 2015-12-29 | 2016-04-20 | 贵州大学 | Method for promoting ecdysis of giant salamander and method for extracting giant salamander genome |
| CN105815425A (en) * | 2016-04-28 | 2016-08-03 | 陕西理工学院 | Giant salamander slaughter machine |
| CN106619739A (en) * | 2016-12-28 | 2017-05-10 | 安顺市四海农业科技开发有限公司 | Artificial collection method of body surface mucus of giant salamander |
| CN106614137A (en) * | 2016-12-19 | 2017-05-10 | 赵华勤 | Collecting method of monopterus albus mucus |
| CN108634235A (en) * | 2018-07-13 | 2018-10-12 | 贵阳学院 | A kind of giant salamander mucus collection device |
| CN109601437A (en) * | 2019-01-25 | 2019-04-12 | 淮海工学院 | The method that Mucilage of loach stress is secreted and collected |
| CN111933093A (en) * | 2020-08-21 | 2020-11-13 | 张家界鲵泰大鲵养殖专业合作社 | Method for preparing musical instrument skin by using artificially-cultured second-generation giant salamander skin and musical instrument skin |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1336383A (en) * | 2000-07-29 | 2002-02-20 | 中国科学院昆明动物研究所 | Giant web bombinin and its prepn. and pharmaceutical application |
| CN102335288A (en) * | 2010-07-20 | 2012-02-01 | 陕西理工学院 | Giant salamander skin mucus scalding ointment |
| CN102764273A (en) * | 2012-08-14 | 2012-11-07 | 新疆师范大学 | Method for purifying skin secretions of tailed amphibian ranodon sibiricus and application |
| CN103829235A (en) * | 2014-01-11 | 2014-06-04 | 陕西理工学院 | Preparation method of giant salamander skin mucus whole powder |
| WO2014110269A1 (en) * | 2013-01-09 | 2014-07-17 | Early Ryanne | Decellularized biomaterial form non-mammalian tissue |
-
2014
- 2014-09-16 CN CN201410471190.5A patent/CN104223121B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1336383A (en) * | 2000-07-29 | 2002-02-20 | 中国科学院昆明动物研究所 | Giant web bombinin and its prepn. and pharmaceutical application |
| CN102335288A (en) * | 2010-07-20 | 2012-02-01 | 陕西理工学院 | Giant salamander skin mucus scalding ointment |
| CN102764273A (en) * | 2012-08-14 | 2012-11-07 | 新疆师范大学 | Method for purifying skin secretions of tailed amphibian ranodon sibiricus and application |
| WO2014110269A1 (en) * | 2013-01-09 | 2014-07-17 | Early Ryanne | Decellularized biomaterial form non-mammalian tissue |
| CN103829235A (en) * | 2014-01-11 | 2014-06-04 | 陕西理工学院 | Preparation method of giant salamander skin mucus whole powder |
Non-Patent Citations (1)
| Title |
|---|
| 全炳昭等: "两栖类动物的麻醉", 《中国兽医杂志》 * |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105494257A (en) * | 2015-12-29 | 2016-04-20 | 贵州大学 | Method for promoting ecdysis of giant salamander and method for extracting giant salamander genome |
| CN105494257B (en) * | 2015-12-29 | 2018-05-22 | 贵州大学 | The method for promoting the method for giant salamander husking and extracting giant salamander genome |
| CN105815425A (en) * | 2016-04-28 | 2016-08-03 | 陕西理工学院 | Giant salamander slaughter machine |
| CN105815425B (en) * | 2016-04-28 | 2017-11-14 | 陕西理工学院 | Giant salamander slaughters machine |
| CN106614137A (en) * | 2016-12-19 | 2017-05-10 | 赵华勤 | Collecting method of monopterus albus mucus |
| CN106619739A (en) * | 2016-12-28 | 2017-05-10 | 安顺市四海农业科技开发有限公司 | Artificial collection method of body surface mucus of giant salamander |
| CN106619739B (en) * | 2016-12-28 | 2020-06-30 | 安顺市四海农业科技开发有限公司 | Artificial collection method of giant salamander surface mucus |
| CN108634235A (en) * | 2018-07-13 | 2018-10-12 | 贵阳学院 | A kind of giant salamander mucus collection device |
| CN108634235B (en) * | 2018-07-13 | 2024-02-09 | 贵阳学院 | Giant salamander mucus collection device |
| CN109601437A (en) * | 2019-01-25 | 2019-04-12 | 淮海工学院 | The method that Mucilage of loach stress is secreted and collected |
| CN109601437B (en) * | 2019-01-25 | 2021-06-15 | 淮海工学院 | Method for stress secretion and collection of loach mucus |
| CN111933093A (en) * | 2020-08-21 | 2020-11-13 | 张家界鲵泰大鲵养殖专业合作社 | Method for preparing musical instrument skin by using artificially-cultured second-generation giant salamander skin and musical instrument skin |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104223121B (en) | 2017-03-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104223121B (en) | A kind of extraction element of giant salamander Skin mucus and extracting method | |
| CN102846519B (en) | Method for extracting snail mucus | |
| CN208545285U (en) | A kind of intelligence three-grid septic tank | |
| CN105586309A (en) | Method for acquiring safe and effective umbilical cord mesenchymal stem cell | |
| CN102757934B (en) | Construction method of fin cell line of anabarilius grahami | |
| CN102766595B (en) | Construction method for anabarilius grahami cardiac cell line | |
| CN205623668U (en) | Pig house of easy clearance | |
| CN204733735U (en) | Drylot feeding sheep trough | |
| CN208064299U (en) | A kind of home-use environmental protection disinfestations device | |
| CN206024794U (en) | Ally oneself with and arrange pig house convenient to remove excrement | |
| CN204907582U (en) | Good fortune longevity spiral shell ovum collection system | |
| CN103769399A (en) | Device and method for cleaning spiral pipe wall | |
| CN204132174U (en) | A kind of workbench for loach injection | |
| CN206238104U (en) | Close shrimp aquaculture pond dirt trap and dirt collection system | |
| CN205052521U (en) | Half crop rotation farming systems of tilapia mossambica - grass carp | |
| CN206866369U (en) | A kind of zander is female, milter identification device | |
| CN204426302U (en) | One can realize automatic electrostatic dust collection and remove peculiar smell duck cage | |
| CN205941074U (en) | High pressure formula blood fluke cercaria sampling device that breathes in | |
| CN203290064U (en) | Pollutant remover for culture pond | |
| CN110710496A (en) | Method for cleaning and purifying saliva of living leeches with golden edges | |
| CN209185350U (en) | A kind of goat cultivation sheep hurdle | |
| CN204206929U (en) | At the bottom of bionic movement formula marine culture pond, chlorination equipment is received in dirt | |
| CN205018042U (en) | Goat of automatically cleaning groove of searching for food | |
| CN213427847U (en) | Make things convenient for aquaculture to use rotatory waste fitting discharging | |
| CN204307880U (en) | Yellow meal worm cleaning device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |