CN102846519B - Method for extracting snail mucus - Google Patents

Abstract

The invention discloses a method for extracting snail mucus. The method comprises the following steps of selecting and treating snails, extracting pedal gland mucus, filtering, carrying out centrifugal separation purification, carrying out vacuum freeze-drying, packaging and storing. The method can effectively extract pedal gland mucus of pedal gland mucus and can effectively keep activities of various biological enzymes in pedal gland mucus. Through the method, mass-production of snail mucus can be realized and activities of various biological enzymes in snail mucus are kept well. The method has good economic benefits.

Description

A kind of extracting method of Limax mucus

Technical field

The present invention relates to and a kind ofly from Limax pedal gland, extract Limax mucus and effectively preserve the method for various bioenzyme activity in mucus.

Background technology

Limax be a kind of can the animal of artificial breeding, and be rich in natural collagen protein, elastin, glycolic acid, allantoin, glucuronic acid and multivitamin in the mucus of Limax pedal gland secretion, skin deep layer is brought in nutrition contained by these compositions into, can repair skin, can increase the nutrition of skin; Allantoin can supplement the cell regeneration factor, can make skin rapid regeneration.And then recover the softness, smooth, fine and smooth of skin.Limax extract has been applied in cosmetic formulations, meanwhile, further studies confirmation, and Limax extract is at Burn skin and the large scarred skin of process and have significant effect equally in the nursing of the problem skins such as pigmentation.And at present the extractive technique of Limax mucus is low, the many and preservation condition of impurity is high makes that the application of Limax mucus is wide and price is high.

Summary of the invention

The object of the present invention is to provide that a kind of technique is simple, strong operability, purification rate is high and effectively can preserve the Limax mucus extracting method of bioenzyme activity.

Technical scheme provided by the invention is as follows:

A kind of extraction of Limax mucus and store method:

(1) the fresh and alive white jade snail of more than 20 grams is selected;

(2) clean Limax with clear water and to be placed in clean environment airing at normal temperatures;

(3) environment below 28 DEG C, take out Limax electricity consumption and to shock by electricity instantaneously Limax foot, one touches namely from repetition 2 ~ 4 times, floating voltage >1000V and load current <3mA;

(4) access the mucus of Limax pedal gland secretion with vessel, extract the Limax after mucus and need supplement after clear water and concentrated feed raise 6 days and again can extract mucus;

(5) mucus quick-freezing 5 ~ 8 hours in the environment of 0 ~ 5 DEG C of will extract;

(6) repeatedly ground by rubber mill until smash mucus in the working environment below 28 DEG C;

(7) by ground mucus filter screen filtration more than 450 orders in the working environment below 10 DEG C, then be separated more than 12 minutes by the centrifuge of 8000 ~ 10000 revs/min of rotating speeds, the dregs sunk to the bottom are separated;

(8) by the supernatant liquid body vacuum and low temperature lyophilizing after separation 18 hours, Limax mucus water content less than 5%, the Snailase of lyophilizing is rice white powdery;

(9) the Limax mucus after lyophilizing carries out vacuum packaging with high-pressure plastic bag in dry environment.

From foregoing description, Limax mucus extracting method of the present invention is simple to operation, and equipment used is conventional item, can realize industrial volume production, and the Limax mucus produced has the feature of active high, long shelf-life.

Detailed description of the invention

A kind of preferred embodiment of the present invention, selects more than 20 grams fresh and alive white jade snails, and clear water rinses Limax, stirs plastic box during flushing, utilizes current to wash out to be attached to the feces on Crusta Limax and foreign material, and during flushing, care should be used to checks, Limax of checkmating is removed; The white jade snail cleaned up is positioned over room temperature airing in clean environment.

50KG Limax is got in environment below 28 DEG C, with voltage >1000V and the electric shock instrument of electric current <3mA shocks by electricity instantaneously Limax foot, during electric shock one touch from, repeatedly shock by electricity 3 times, now Limax pedal gland can secrete mucus, collects in container with little spoon by Limax mucus, and the white jade snail of 50KG can collect the mucus of 5600g, the Limax of having extracted mucus is put back to rearing-box and supplements clear water and concentrated feed, feed and can again extract after 7 days; By the quick-freezing 8 hours in the environment of 0 ~ 5 DEG C of the Limax mucus collected.

Take out the mucus after quick-freezing, repeatedly ground by rubber mill until smash mucus in the working environment below 28 DEG C;

In environment below 10 DEG C, ground mucus is fallen impurity by filter screen filtration more than 450 orders, the centrifuge being positioned over 10000 revs/min is separated, 12 minutes operating times, then the supernatant liquid of separator well is poured out, lower floor's dregs are abandoned, and the first mucus of getting of 5600g can obtain 5113g milliliter clear liquor;

Isolated supernatant liquid carries out low-temperature freeze drying in plastic containers, vacuum freeze-drying 18 as a child, Snailase water content about 4%, the Snailase after lyophilizing is rice white powdery, can obtain 89 grams of powder;

Limax mucus after lyophilizing carries out vacuum packaging at dry environment high-pressure plastic bag.

Above are only a specific embodiment of the present invention, but technique of the present invention is not limited thereto, all this processes that utilizes carry out the change of unsubstantiality to the present invention, all should belong to the behavior of invading scope.

Claims (1)

1. an extracting method for Limax mucus, is characterized in that:

(1) the fresh and alive white jade snail of more than 20 grams is selected;

(2) clean Limax with clear water and to be placed in clean environment airing at normal temperatures;

(3) environment below 28 DEG C, take out Limax electricity consumption and to shock by electricity instantaneously Limax foot, one touches namely from repetition 2 ~ 4 times, floating voltage >1000V and load current <3mA;

(4) access the mucus of Limax pedal gland secretion with vessel, extract the Limax after mucus and need supplement after clear water and concentrated feed raise 6 days and again can extract mucus;

(5) mucus quick-freezing 5 ~ 8 hours in the environment of 0 ~ 5 DEG C of will extract;

(6) repeatedly ground by rubber mill until smash mucus in the working environment below 28 DEG C;

(7) by ground mucus filter screen filtration more than 450 orders in the working environment below 10 DEG C, then be separated more than 12 minutes by the centrifuge of 8000 ~ 10000 revs/min of rotating speeds, the dregs sunk to the bottom are separated;

(8) by the supernatant liquid body vacuum and low temperature lyophilizing after separation 18 hours, Limax mucus water content less than 5%, the Snailase of lyophilizing is rice white powdery;

(9) the Limax mucus after lyophilizing carries out vacuum packaging with high-pressure plastic bag in dry environment.