CN106892961A - A kind of separating and extracting process of fly-maggot protein polypeptide and fly maggot oil - Google Patents
A kind of separating and extracting process of fly-maggot protein polypeptide and fly maggot oil Download PDFInfo
- Publication number
- CN106892961A CN106892961A CN201710078799.XA CN201710078799A CN106892961A CN 106892961 A CN106892961 A CN 106892961A CN 201710078799 A CN201710078799 A CN 201710078799A CN 106892961 A CN106892961 A CN 106892961A
- Authority
- CN
- China
- Prior art keywords
- maggot
- oil
- fly
- protein
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/001—Refining fats or fatty oils by a combination of two or more of the means hereafter
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/16—Refining fats or fatty oils by mechanical means
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B7/00—Separation of mixtures of fats or fatty oils into their constituents, e.g. saturated oils from unsaturated oils
- C11B7/0075—Separation of mixtures of fats or fatty oils into their constituents, e.g. saturated oils from unsaturated oils by differences of melting or solidifying points
Abstract
A kind of separating and extracting process of fly-maggot protein and fly maggot oil; chemical method is substituted with physical method; without organic solvent, with better simply technique, under cryogenic; fly-maggot protein and fly maggot oil height are dissociated; improve the content of fly-maggot protein and keep its active ingredient, the fractional saturation fat in fly maggot oil is removed, improve the content containing unrighted acid in fly maggot oil; strengthen its activity, be that the active fly-maggot protein of industrially scalable metaplasia product and fly maggot oil provide practicable foundation.
Description
Technical field
The invention belongs to biological technical field, the separation and Extraction side of more particularly to a kind of fly-maggot protein polypeptide and fly maggot oil
Method.
Background technology
The sixties in last century, to solve the shortage of edible and feed protein resource, many country's research and development cultured insects
Industry extracts insect protein, in cultured insect industry is developed, preferably goes out housefly breeding production fly maggot to extract protein, because
For the extensive intensive, breeding of cultivation housefly energy is fast, yield is big, and breeding feed wide material sources are cheap, more concerned,
These environomental pollution sources such as the leftover bits and pieces that agricultural byproducts processing is abandoned, rotten grain and food, excrement of livestock culture excretion, all
Can as fly maggot produce compost, in other words these pollution sources can all be processed with fly maggot, process these pollution sources with fly maggot,
Treating capacity is big, and speed is fast, without being processed and can not be reached with other method the features such as pollution again, while the metabolism of fly maggot is produced
Thing --- maggot excrement is the biological organic fertilizer of very high-quality, therefore, cultivation housefly production fly maggot both obtains protein-rich product, solves again
Certainly problem of environmental pollution, with the further investigation to housefly cultivation and fly maggot deep processing, fly maggot is controlled by cultivating real cooperation
The content of protein and fat, by the immune antibacterial activity of the special induction raising fly maggot product to fly maggot, therefore the life of fly maggot
Produce and deep processing turns into one of focus of current biological industry research.
Extraction on fly maggot oil, up to the present, there is following several method from disclosed documents and materials：
1st, organic solvent extractionprocess：Clean fly maggot will be cleaned in air blast or drying baker, temperature is low 60 DEG C, drying, or true
Reciprocal of duty cycle is 0.01/MPQ, and 60 DEG C of temperature control is dried, and makes the moisture content of fly maggot low by 16%, is then crushed to 80 mesh, obtains fly maggot and does
Powder, with acetone or 60 --- 90 petroleum ethers press W/V1:3, soak at room temperature 24 hours to 36 hours in closed container are placed in, or use rope
Family name's digester flows back 12 hours to 20 hours, then filters precipitation, and filter residue obtains defatted protein powder in dry, and filtrate is through low-temperature evaporation
Returnable obtains fly maggot oil.
2nd, milling process：By low temperature drying and the maggot powder of 80 mesh is crushed to, bakes heat to 50 DEG C -55 DEG C, use small oil press pressure
Squeeze, the withered slag that squeezing extracts after first oil is crushed to 80 mesh again, is placed in and steam is passed through in mixer, granulated slag is absorbed water uniform,
Moisture content reaches 20% -25%, in 45 DEG C of -50 DEG C of vexed 30miN of heat, so carries out the 2nd squeezing, will twice squeeze and carry and maggot oil
The residue being mixed into is filtered to remove, oil strain through exclusion moisture content is evaporated under reduced pressure, obtains final product maggot oil again.
3rd, boiling centrifugal process：After fresh fly maggot is cleaned into removal of impurities, fly maggot body is killed in pH value not 10-11 alkaline solution
The parasite of table and harmful microorganism, in feeding boiling vessel, in 80 DEG C of -95 DEG C of boiling 15miN, digested fly maggot are carried out
Mashing, slurries are centrifuged, and obtain solid content, and the oil phase containing solid content is again centrifuged the oil phase containing solid content, must consolidate
Shape thing and profit phase, maggot oil is obtained by profit phase mixture evaporative removal moisture content.
4th, supercritical ultrasonics technology：By the maggot powder of low temperature drying, by solid-liquid 1:20—1:25 are diluted to suspension, with ultrasound to suspending
Liquid processes 20 miN, is then centrifuged for isolating maggot oil.
1,2,3 methods of the above method, lab scale checking is carried out with the maggot powder of the same race of low temperature drying, obtains following situation：It is molten
Agent extraction, oil yield highest reaches 92%, and the impurity of oil is few, and the solvent of gained defatted protein powder residual is extremely difficult to be eliminated, solvent
Consumption is up to 25%, and the time is long.Milling process, the maggot oil for raising is very limpid, and unsaturated fat content is high, reaches 75.5%, oil yield compared with
It is low, it is low to 76%, there is a small amount of fly-maggot protein brown stain.Boiling centrifugal process, oil yield is low, and it is existing that solidification occurs since 65 DEG C in protein
As, most solidification more than 75 DEG C, although the protein that is broken after mashing can not be separated very well with oil, the oil activity for obtaining is low.
Supercritical ultrasonics technology does not do lab scale, but theoretically judges, oil yield will not be very high, and protein denaturation and maggot can be caused to aoxidize, because
During for ultrasonication suspension, the cavitation particle in solution is more, and dry the frying of cavitation is local generation HTHP, and temperature reaches Baidu
More than, up to tens air pressure, biggest advantage is short process time to pressure.
For disadvantages mentioned above, the purpose of technological innovation of the invention is that to provide a kind of process is simple, recovery rate high, suitable
Fly-maggot protein polypeptide and fly maggot the oil extraction method of industrial-scale production.
The content of the invention
A kind of separating and extracting process of fly-maggot protein polypeptide and fly maggot oil, including：
（1）Fresh maggot is soaked into 5-10min with 3 ‰ -5 ‰ liquor potassic permanganate, maggot body is cleaned with running water and is removed permanganic acid
Potassium, then 3% common salt aqueous solution immersion 5min is put into, pick up, it is standby after the salt with clear water flushing maggot body；
（2）By standby fly maggot, maggot skin is racked with meat mincer, maggot is starched outflow；
（3）The maggot that will be crushed with meat mincer is starched, and is ground with colloid mill, and 3 times 3% of NaoH is added by fresh maggot weight during grinding
Solution makes pH value reach 9.3 alkali lye to be ground homogenate, and the fineness of grinding reaches whole slurries by 40 mesh sieves；
（4）Ground maggot slurry input microwave frequency in the microwave tank of 2450MHZ, microwave 15-30min, 60 DEG C of temperature control, and
Keep this warm 30 min；
（5）Solution after microwave is taken out, is filtered with 200 mesh filter cloth tripod pendulum type batch centrifugals, leach maggot skin, maggot eye and maggot mouth, filter residue
Kept dry is collected, chitin raw material is produced.
（6）Slurries obtained by upper step is filtered, with supercentrifuge, in rotating speed 78000r/miN centrifugations, obtain solid on a small quantity
Body slag, the heavy-fluid containing protein and maggot oil light liquid；
（7）The maggot oil light liquid that will be obtained, by volume 1:1 soft water for adding 50 DEG C -60 DEG C, be sufficiently mixed washing maggot oil, at a high speed from
The heart, centrifugal speed 71000r/miN obtains the light liquid of heavy-fluid and oil mixing with water, and heavy-fluid merges with the heavy-fluid of step 6, and light liquid is sub-packed in
In tray container, liquid depth control within 150mm, be placed in refrigerator-freezer, by light liquid be chilled to -32 DEG C and holding 2 hours, so
After take out naturally to thaw；
（8）By step 6 and 7 heavy-fluids for merging, neutralized with the chitosan solution of 15% acetate dissolution 1.5%, pH value is reached 5.0, will
Protein precipitating, after being filtered with 400 mesh filter cloth tripod pendulum type batch centrifugals after solution layering, collects filter residue, then reduced vacuum is dried, temperature
40-60 DEG C of control, size-reduced machine is crushed to 100 mesh, obtains degreasing Flyblow albumen powder；
（9）By step 7 through the light liquid of the oil mixing with water of fresh jelly, with separatory funnel water-oil separating, thick maggot oil is obtained；
（10）By thick maggot oil tube centrifuge, in 78000r/miN centrifugation 15-20 min, along length of tube clearly visible four
The different material of layer, the superiors are the limpid transparent maggot oil of micro- salmon pink, and the 2nd layer is the limpid transparent maggot oil of light olive, the 3rd
Layer is the opaque fly maggot oil of milky, and the 4th layer is water and a small amount of solid impurity, and fly maggot oil is obtained after point liquid.Except three layers of oil
Outside face color is differed, its proportion is also differed, and the 1st layer of proportion of oil exists in 0.8g/mt or so, the proportion of the 2nd layer of oil
0.85g/mt -0.9g/mt or so, the 3rd layer of proportion >=0.9g/mt of oil.
In preferred mode, described supercentrifuge is tubular type or dish-style supercentrifuge.
Chemical method is substituted with physical method, without organic solvent, with better simply technique, under cryogenic, by fly
Maggot protein is dissociated with fly maggot oil height, is improved the content of fly-maggot protein and is kept its active ingredient, and the part in fly maggot oil is satisfied
Removed with fat, improve the content containing unrighted acid in fly maggot oil, strengthen its activity, this raw materials technology utilization rate is high, main
It is fly-maggot protein and fly maggot oil to extract product, and byproduct can be used for producing chitin or as fertilizer additive, there is provided
Protein.
According to analysis, containing protein from 45% -65% in fly maggot dry powder, fat content is from 12% -36%, and mobility scale is larger,
Research shows, the protein and fat content in the protein and fat content of fly maggot and fly maggot compost are proportionate, therefore,
The content of its protein and fat can be controlled by the appropriate cooperation of fly maggot compost.
Experiment shows：When the oil content of fly maggot powder reaches more than 13%, if maggot oil is not separated, production fly maggot is given
When full-cream protein powder or defatted protein powder or manufacture fly-maggot protein peptide, great trouble is caused to product drying, therefore, work as fly maggot
（It is dry）Oil content must separate maggot oil when reaching more than 13%, and this is the requirement in production technology, and is examined from economic benefit
Consider, isolate maggot oil, the added value of enterprise is improved.
The effect of fly maggot oil：1st, fly maggot oil is the important source material of manufacture treatment hypercholesterolemia and fatty liver medicament.2、
Unrighted acid therein, for saturated fatty acid structure is mutually intended, can purify blood with deep sea fish oil, remove blood rubbish.3、
It is that skin burn, scald, chemicals are burnt medicine evident in efficacy, to skin irritatin and allergic reaction.4th, to Pseudomonas aeruginosa,
There is gold-coloured staphylococci strong inhibition to act on.5th, it is to kill the driving and killing agent that harm grain beans store " confused flour beetle " insect.6、
In with dibastic sodium phosphate as buffer solution, neutral lipase is added, fly maggot oil is digested, obtain the acid of fly maggot oil fat, fly maggot
Fatty acid oil has good suppression and therapeutic action to the integrase and reverse transcriptase of human body AIDS.
Notable beneficial effect of the invention is：
1st, chemical method is substituted with physical method, separates fly-maggot protein matter and fly maggot oil, separated each operation and enter under cryogenic
Row can very well keep the activity of the fly-maggot protein of gained and the stability of fly maggot oil.The high-quality for obtaining manufacture chitin simultaneously is former
Material.
2nd, this method separates protein reason fat, and without HTHP and highly basic strong acid, technique is simpler, it is easy to operate,
It is adapted to industrial-scale production, production security is high.
3rd, this method is unjust is applied to that fly-maggot protein is oily with fly maggot to be separated, be also suitable earthworm,
The protein of oyster, animal brain etc. is separated with fat, and such as the protein of Moringa seed is separated with Moringa seed oil.
Specific embodiment
Embodiment 1：
The separating and extracting process of fly-maggot protein polypeptide and fly maggot oil, including：
（1）Fresh maggot is soaked into 10min with 3 ‰ liquor potassic permanganate, maggot body is cleaned with running water and is removed potassium permanganate, then put into
3% common salt aqueous solution immersion 5min, picks up, standby after the salt with clear water flushing maggot body；
（2）By standby fly maggot, maggot skin is racked with meat mincer, maggot is starched outflow；
（3）The maggot that will be crushed with meat mincer is starched, and is ground with colloid mill, and 3 times 3% of NaoH is added by fresh maggot weight during grinding
Solution makes pH value reach 9.3 alkali lye to be ground homogenate, and the fineness of grinding reaches whole slurries by 40 mesh sieves；
（4）Ground maggot slurry input microwave frequency in the microwave tank of 2450MHZ, microwave 15min, 60 DEG C of temperature control, and protect
Hold this warm 30 min；
（5）Solution after microwave is taken out, is filtered with 200 mesh filter cloth tripod pendulum type batch centrifugals, leach maggot skin, maggot eye and maggot mouth, filter residue
Kept dry is collected, chitin raw material is produced.
（6）Slurries obtained by upper step is filtered, with supercentrifuge, in rotating speed 78000r/miN centrifugations, obtain solid on a small quantity
Body slag, the heavy-fluid containing protein and maggot oil light liquid；
（7）The maggot oil light liquid that will be obtained, by volume 1:1 soft water for adding 50 DEG C, is sufficiently mixed and washs maggot oil, high speed centrifugation, from
Heart speed 71000r/miN, obtains the light liquid of heavy-fluid and oil mixing with water, and heavy-fluid merges with the heavy-fluid of step 6, and light liquid is sub-packed in tray
In container, liquid depth is controlled within 150mm, is placed in refrigerator-freezer, light liquid is chilled into -32 DEG C and is kept for 2 hours, Ran Houqu
Go out naturally to thaw；
（8）By step 6 and 7 heavy-fluids for merging, neutralized with the chitosan solution of 15% acetate dissolution 1.5%, pH value is reached 5.0, will
Protein precipitating, after being filtered with 400 mesh filter cloth tripod pendulum type batch centrifugals after solution layering, collects filter residue, then reduced vacuum is dried, temperature
40-60 DEG C of control, size-reduced machine is crushed to 100 mesh, obtains degreasing Flyblow albumen powder；
（9）By step 7 through the light liquid of the oil mixing with water of fresh jelly, with separatory funnel water-oil separating, thick maggot oil is obtained；
（10）By thick maggot oil tube centrifuge, in 78000r/miN centrifugation 15-20 min, along length of tube clearly visible four
The different material of layer, the superiors are the limpid transparent maggot oil of micro- salmon pink, and the 2nd layer is the limpid transparent maggot oil of light olive, the 3rd
Layer is the opaque fly maggot oil of milky, and the 4th layer is water and a small amount of solid impurity, and fly maggot oil is obtained after point liquid.Except three layers of oil
Outside face color is differed, its proportion is also differed, and the 1st layer of proportion of oil exists in 0.8g/mt or so, the proportion of the 2nd layer of oil
0.85g/mt -0.9g/mt or so, the 3rd layer of proportion >=0.9g/mt of oil.
Described supercentrifuge is tubular type or dish-style supercentrifuge.
Embodiment 2：
The separating and extracting process of fly-maggot protein polypeptide and fly maggot oil, including：
（1）Fresh maggot is soaked into 10min with 5 ‰ liquor potassic permanganate, maggot body is cleaned with running water and is removed potassium permanganate, then put into
3% common salt aqueous solution immersion 5min, picks up, standby after the salt with clear water flushing maggot body；
（2）By standby fly maggot, maggot skin is racked with meat mincer, maggot is starched outflow；
（3）The maggot that will be crushed with meat mincer is starched, and is ground with colloid mill, and 3 times 3% of NaoH is added by fresh maggot weight during grinding
Solution makes pH value reach 9.3 alkali lye to be ground homogenate, and the fineness of grinding reaches whole slurries by 40 mesh sieves；
（4）Ground maggot slurry input microwave frequency in the microwave tank of 2450MHZ, microwave 20min, 60 DEG C of temperature control, and protect
Hold this warm 30 min；
（5）Solution after microwave is taken out, is filtered with 200 mesh filter cloth tripod pendulum type batch centrifugals, leach maggot skin, maggot eye and maggot mouth, filter residue
Collect kept dry；
（6）Slurries obtained by upper step is filtered, with supercentrifuge, in rotating speed 78000r/miN centrifugations, obtain a small amount of solid slag,
Heavy-fluid and maggot oil light liquid containing protein；
（7）The maggot oil light liquid that will be obtained, by volume 1:1 soft water for adding 60 DEG C, is sufficiently mixed and washs maggot oil, high speed centrifugation, from
Heart speed 71000r/miN, obtains the light liquid of heavy-fluid and oil mixing with water, and heavy-fluid merges with the heavy-fluid of step 6, and light liquid is sub-packed in tray
In container, liquid depth is controlled within 150mm, is placed in refrigerator-freezer, light liquid is chilled into -32 DEG C and is kept for 2 hours, Ran Houqu
Go out naturally to thaw；
（8）By step 6 and 7 heavy-fluids for merging, neutralized with the chitosan solution of 15% acetate dissolution 1.5%, pH value is reached 5.0, will
Protein precipitating, after being filtered with 400 mesh filter cloth tripod pendulum type batch centrifugals after solution layering, collects filter residue, then reduced vacuum is dried, temperature
40-60 DEG C of control, size-reduced machine is crushed to 100 mesh, obtains degreasing Flyblow albumen powder；
（9）By step 7 through the light liquid of the oil mixing with water of fresh jelly, with separatory funnel water-oil separating, thick maggot oil is obtained；
（10）By thick maggot oil tube centrifuge, in the min of 78000r/miN centrifugations 20, clearly visible four layers along length of tube
Different materials, the superiors are the limpid transparent maggot oil of micro- salmon pink, and the 2nd layer is the limpid transparent maggot oil of light olive, the 3rd layer
It is the opaque fly maggot oil of milky, the 4th layer is water and a small amount of solid impurity, and fly maggot oil is obtained after point liquid.
Described supercentrifuge is tubular type or dish-style supercentrifuge.
Claims (2)
1. a kind of fly-maggot protein polypeptide and fly maggot oil separating and extracting process, it is characterised in that including：
（1）Fresh maggot is soaked into 5-10min with 3 ‰ -5 ‰ liquor potassic permanganate, maggot body is cleaned with running water and is removed permanganic acid
Potassium, then 3% common salt aqueous solution immersion 5min is put into, pick up, it is standby after the salt with clear water flushing maggot body；
（2）By standby fly maggot, maggot skin is racked with meat mincer, maggot is starched outflow；
（3）The maggot that will be crushed with meat mincer is starched, and is ground with colloid mill, and 3 times 3% of NaoH is added by fresh maggot weight during grinding
Solution makes pH value reach 9.3 alkali lye to be ground homogenate, and the fineness of grinding reaches whole slurries by 40 mesh sieves；
（4）Ground maggot slurry input microwave frequency in the microwave tank of 2450MHZ, microwave 15-30min, 60 DEG C of temperature control, and
Keep this warm 30 min；
（5）Solution after microwave is taken out, is filtered with 200 mesh filter cloth tripod pendulum type batch centrifugals, leach maggot skin, maggot eye and maggot mouth, filter residue
Collect kept dry；
（6）Slurries obtained by upper step is filtered, with supercentrifuge, in rotating speed 78000r/miN centrifugations, obtain a small amount of solid slag,
Heavy-fluid and maggot oil light liquid containing protein；
（7）The maggot oil light liquid that will be obtained, by volume 1:1 soft water for adding 50 DEG C -60 DEG C, be sufficiently mixed washing maggot oil, at a high speed from
The heart, centrifugal speed 71000r/miN obtains the light liquid of heavy-fluid and oil mixing with water, and heavy-fluid merges with the heavy-fluid of step 6, and light liquid is sub-packed in
In tray container, liquid depth control within 150mm, be placed in refrigerator-freezer, by light liquid be chilled to -32 DEG C and holding 2 hours, so
After take out naturally to thaw；
（8）By step 6 and 7 heavy-fluids for merging, neutralized with the chitosan solution of 15% acetate dissolution 1.5%, pH value is reached 5.0, will
Protein precipitating, after being filtered with 400 mesh filter cloth tripod pendulum type batch centrifugals after solution layering, collects filter residue, then reduced vacuum is dried, temperature
40-60 DEG C of control, size-reduced machine is crushed to 100 mesh, obtains degreasing Flyblow albumen powder；
（9）By step 7 through the light liquid of the oil mixing with water of fresh jelly, with separatory funnel water-oil separating, thick maggot oil is obtained；
（10）By thick maggot oil tube centrifuge, in 78000r/miN centrifugation 15-20 min, along length of tube clearly visible four
The different material of layer, the superiors are the limpid transparent maggot oil of micro- salmon pink, and the 2nd layer is the limpid transparent maggot oil of light olive, the 3rd
Layer is the opaque fly maggot oil of milky, and the 4th layer is water and a small amount of solid impurity, and fly maggot oil is obtained after point liquid.
2. fly-maggot protein polypeptide according to claim 1 and fly maggot oil separating and extracting process, it is characterised in that：Described
Supercentrifuge is tubular type or dish-style supercentrifuge.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710078799.XA CN106892961A (en) | 2017-02-14 | 2017-02-14 | A kind of separating and extracting process of fly-maggot protein polypeptide and fly maggot oil |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710078799.XA CN106892961A (en) | 2017-02-14 | 2017-02-14 | A kind of separating and extracting process of fly-maggot protein polypeptide and fly maggot oil |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106892961A true CN106892961A (en) | 2017-06-27 |
Family
ID=59198625
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710078799.XA Pending CN106892961A (en) | 2017-02-14 | 2017-02-14 | A kind of separating and extracting process of fly-maggot protein polypeptide and fly maggot oil |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106892961A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107142186A (en) * | 2017-07-10 | 2017-09-08 | 罗乌支 | A kind of preparation method of the sub- native peptides wine of narcissus |
| CN107183459A (en) * | 2017-07-10 | 2017-09-22 | 罗乌支 | A kind of preparation method of narcissus homogenate peptide liquid |
| CN107897943A (en) * | 2017-11-28 | 2018-04-13 | 苏洁 | A kind of method that deer bone native peptides are extracted in the bone from deer |
| CN107904011A (en) * | 2017-11-16 | 2018-04-13 | 罗乌支 | A kind of method that fly maggot crude oil is separated into 4 kinds of fly maggot oil |
| CN107903300A (en) * | 2017-11-16 | 2018-04-13 | 罗乌支 | A kind of method that native peptides are extracted from fly |
| CN108850529A (en) * | 2018-06-07 | 2018-11-23 | 苏州康尧环保科技有限公司 | A kind of fly maggot disinfection cleaning process flow and disinfection cleaning system |
| CN113792993A (en) * | 2021-08-25 | 2021-12-14 | 浙江省农业科学院 | Preservation and preservation effect evaluation method of maggot protein |
| CN115109641A (en) * | 2022-07-07 | 2022-09-27 | 浙江科峰生物技术有限公司 | Method for separating and extracting Tabanus protein and oil |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101019603A (en) * | 2007-03-21 | 2007-08-22 | 李金穗 | Process of producing active protein and oil with maggot and earth worm |
| CN105385739A (en) * | 2015-12-09 | 2016-03-09 | 梁尚文 | Method for extracting protein peptide from poecilobdella manillensis |
-
2017
- 2017-02-14 CN CN201710078799.XA patent/CN106892961A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101019603A (en) * | 2007-03-21 | 2007-08-22 | 李金穗 | Process of producing active protein and oil with maggot and earth worm |
| CN105385739A (en) * | 2015-12-09 | 2016-03-09 | 梁尚文 | Method for extracting protein peptide from poecilobdella manillensis |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107142186A (en) * | 2017-07-10 | 2017-09-08 | 罗乌支 | A kind of preparation method of the sub- native peptides wine of narcissus |
| CN107183459A (en) * | 2017-07-10 | 2017-09-22 | 罗乌支 | A kind of preparation method of narcissus homogenate peptide liquid |
| CN107904011A (en) * | 2017-11-16 | 2018-04-13 | 罗乌支 | A kind of method that fly maggot crude oil is separated into 4 kinds of fly maggot oil |
| CN107903300A (en) * | 2017-11-16 | 2018-04-13 | 罗乌支 | A kind of method that native peptides are extracted from fly |
| CN107897943A (en) * | 2017-11-28 | 2018-04-13 | 苏洁 | A kind of method that deer bone native peptides are extracted in the bone from deer |
| CN108850529A (en) * | 2018-06-07 | 2018-11-23 | 苏州康尧环保科技有限公司 | A kind of fly maggot disinfection cleaning process flow and disinfection cleaning system |
| CN113792993A (en) * | 2021-08-25 | 2021-12-14 | 浙江省农业科学院 | Preservation and preservation effect evaluation method of maggot protein |
| CN115109641A (en) * | 2022-07-07 | 2022-09-27 | 浙江科峰生物技术有限公司 | Method for separating and extracting Tabanus protein and oil |
| CN115109641B (en) * | 2022-07-07 | 2023-11-24 | 浙江科峰生物技术有限公司 | Tabanus protein and grease separation and extraction method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106892961A (en) | A kind of separating and extracting process of fly-maggot protein polypeptide and fly maggot oil | |
| CN104957448B (en) | A kind of method of cadmium in molten combination fermentation removing rice of acid | |
| CN102199582B (en) | Method for extraction and preservation of helicase | |
| CN104829734B (en) | A kind of method of utilization dried thin mushroom production pigment, albumen, polysaccharide and dietary fiber | |
| CN102766530A (en) | Method for extracting phospholipid-rich krill oil from Antarctic krill | |
| CN106281638B (en) | A kind of method that camellia seed oil and oil tea saponin and camellia seed meal feed are extracted from camellia seed kernel | |
| CN103302080A (en) | Preparation method of eggshell powder and processing device | |
| CN102242093A (en) | Comprehensive development method of high value-added active components of eggs | |
| CN102763761A (en) | Method for preparing enzymolysis low-sodium soybean isolated protein | |
| CN101548760A (en) | Technique for preparing glucoraphanin-riched broccoli concentrated powder | |
| CN103396878A (en) | Preparation method of low-temperature cold-pressed peanut oil | |
| CN102702384A (en) | Method for removing proteins in chitin material | |
| CN104726197B (en) | The method of extracting cigarette seed oil is discarded in cigarette seed using homogenate extraction technology from Turkish tobaccos | |
| RU2432956C1 (en) | Method of complex processing of echinus | |
| CN108948124A (en) | A kind of preparation method of Tea Saponin | |
| CN107259293A (en) | A kind of method that natural is prepared from clam worm | |
| CN107805273A (en) | A kind of method that native peptides are extracted from fly maggot | |
| CN103160392A (en) | Method for preparing non-toxic fruit and vegetable detergent | |
| CN101292670B (en) | Method for preparing plant jatropha curcas insecticide | |
| CN105712868B (en) | Fruits and vegetables natural organic acids calcium synthetic method using shell as calcium source | |
| CN100384989C (en) | Collecting method for insect virus breed | |
| CN105884929A (en) | Method of extracting pectin from premna microphylla leaves | |
| CN102138679A (en) | Technique for processing and preparing kelp seaweed spore powder | |
| CN105439864A (en) | Method for extracting high-purity ferulic acid from Szechwan lovage rhizome stems | |
| CN105537248A (en) | Comprehensive utilization method of banana stems and leaves |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170627 |
|
| RJ01 | Rejection of invention patent application after publication |