CN107903300A - A kind of method that native peptides are extracted from fly - Google Patents
A kind of method that native peptides are extracted from fly Download PDFInfo
- Publication number
- CN107903300A CN107903300A CN201711140091.9A CN201711140091A CN107903300A CN 107903300 A CN107903300 A CN 107903300A CN 201711140091 A CN201711140091 A CN 201711140091A CN 107903300 A CN107903300 A CN 107903300A
- Authority
- CN
- China
- Prior art keywords
- fly
- gained
- filtrate
- method described
- concentration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Water Supply & Treatment (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention discloses a kind of method that native peptides are extracted from fly, fly body surface is sterilized and is dehydrated to body surface no moisture for (1) by operating procedure;(2) water mill slurrying, sieving are added;(3) gained filtrate adjusts pH value and is starched into alkaline fly after filtering, and 25~30min of microwave treatment, 40min is kept the temperature between 55~60 DEG C;(4) cool down, adjust pH value, stand, filtered after being layered, filtrate carries out micro-filtration, two-stage ultrafiltration;(5) nanofiltration is filtered, the concentration of gained filtrate;(6) concentrate is discharged, and adds activated carbon decolorizing, filtering, removes activated carbon;(7) discharge is cooled to room temperature;(8) gained ice cube takes out after when will be refrigerated to 37 DEG C and small holding 1~2, dry, up to product.The method of the present invention operation is succinct, and the extraction gained native peptides rate of recovery is up to 77.33%, substantially increases the rate of recovery of native peptides;The method of the present invention is suitable for big industrialized production, and flow is succinct, easy to operate.
Description
Technical field
The present invention relates to the extracting method of native peptides, more particularly to a kind of method that native peptides are extracted from fly.
Background technology
In the last hundred years, many diseases that the mankind suffer from, but due to the invention and development of antibiotic, cure many patients, make to resist
Raw element reach in the status of the world of medicine and effect be difficult to discuss than stage.But over time, antibiotic is widely made
With its negative effect increasingly shows, as soon as being only its drug resistance, its unit administration amount is more used higher, until antibiotic is not
Body is acted again.Some external developed countries have strictly regulated management and have used before more than ten years, and China is also begun in recent years
The use of stringent management and control antibiotics.Why is patient when people worry to can use without medicine while antibiotic effect is reappraised
Do, if there is alternative medicine to useUnder the effort of numerous scientists, find it is a kind of than antibiotic better efficacy and
Novel drugs --- the antibacterial peptide of safety.Antibacterial peptide is different from our daily antibiotic used, it does not have drug resistance and specificity,
Antibacterial peptide is a kind of non-specific immune response product, has broad spectrum activity, and being not only resistant to various bacteria, fungi can also antigen
Worm, virus even antitumor cell.Antibacterial peptide is that scientists a certain position first out of animal body is separated, according to report
Road, has a kind of antimicrobial active protein to have extremely strong sterilization and disease resistance in the secretion of fly, as long as 0.01% concentration
It is that a kind of antibiotic all can not be by comparison in the prior art with regard to various bacteria and virus can be caused dead.
Fly can be rated as senior statesman's level animal on the earth, and according to having nearly 400,000,000 years on the history of existence of textual criticism fly, fly is dynamic
Classification in thing, belongs to Arthropoda, Insecta, Diptera, a major class animal of Aristocera.Including drosophila
There are several fly categories in section, Anthomyiidae, Flesh flies, Calliphoridae and Nuscidae etc., each section again.China be most commonly that housefly, city fly,
False stable fly, Fannia canicularis, first latrine fly, chrysomyia megacephala, lucilia sericata, lucilia cuprina, calliphora erythrocephala and volt fly etc., these above-mentioned flies
Breeding ground is more on human and animal excreta and organic waste and rotten plant, abounds with the phase in season of the summer and autumn compared with warm.Fly maggot breeding
If owner housefly, secondly big pipunculid, because to enter house most close with human contact for housefly, intensive can propagate artificially, greatly
Though it is more with human contact nearby that pipunculid does not enter house but its scope of activities is all lived in crowd, also can intensive culture through training.
Existing native peptides amount in fly body, according to detection, the ecological environment with settlement, the material of feeding, the factor such as fly kind species is related,
With season without significant difference.Ecological condition is more severe, and the material of feeding is more corrupt, and the natural peptide content of body meat is higher, same to batten
Natural peptide content under part in big pipunculid body is more than housefly, and the content of wild housefly native peptides produces what maggot was eliminated than artificial breeding
Hylemyia Platura Meigen is more.When to extract fly native peptides as industry, these factors merit attention, but have a common feature, no matter containing day
How much is right peptide amount, their the peptide fragment regularity of distribution is relatively.
Extraction native peptides are extracted from fly maggot mostly in the prior art, and extracting method is complicated, of high cost, and extract gained day
Right peptide yield is not high, therefore, native peptides convenience, succinct, high income method is extracted from fly there is an urgent need for a kind of at present.
The information for being disclosed in the background section is merely intended to understanding of the increase to the general background of the present invention, without answering
It has been the prior art well known to persons skilled in the art when being considered as recognizing or implying the information structure in any form.
The content of the invention
The present invention extracts the method for native peptides in order to solve the above technical problems, invention is a kind of from fly, so as to solve existing
There is the problem of extracting method is complicated, of high cost, extraction native peptides yield is low in technology.
To achieve the above object, technical solution provided by the invention is as follows:
A kind of method that native peptides are extracted from fly, includes following operating procedure:
(1) fly body surface is sterilized and be dehydrated to body surface no moisture;
(2) gained fly after step (1) dehydration, by solid-liquid mass ratio 1:4 addition deionized waters grind slurry, gained slurries mistake
Sieve, gained filter residue dry the raw material of additive capable of using as feed or chitin processed;
(3) gained filtrate adjusting pH value is starched into alkaline fly after sieving in step (2), and 60 DEG C of microwave treatments 25 of temperature control~
30min, 40min is kept the temperature between 55~60 DEG C;
(4) the fly slurry discharge that microwave treatment is crossed in step (3) is cooled down, adjusting pH value to 4.8~5.0, precipitating protein,
Polysaccharide, fat etc., stand, are filtered after being layered, filter residue low temperature drying, obtains fly albumen powder;Filtrate carries out micro-filtration, two-stage successively
Ultrafiltration removes macromolecular substances;
(5) gained filtrate reverse osmosis membrane, i.e. nanofiltration are filtered after ultrafiltration in step (4), and gained filter residue is low price salt, is removed
Low price salt, the concentration of gained filtrate;
(6) gained concentrate after concentration in step (5) is discharged, adds granular pattern activated carbon decolorizing, filtering, except deactivation
Charcoal;
(7) gained filtrate discharge after filtering in step (6) is cooled to room temperature, sends to refrigerator-freezer or freezer freezing;
(8) gained ice cube takes out after when will be refrigerated to -37 DEG C and small holding 1~2, breaks into fritter microwave vacuum dryer
It is dry, up to product.
Preferably, the fly described in step (1) is trapping wild housefly or big pipunculid, or artificial feeding is educated maggot and eliminated
At least one of Hylemyia Platura Meigen.
Preferably, the disinfection described in step (1) rinses for 3.5 ‰ liquor potassic permanganate of fly fly body mass concentration
12min is picked up, then the potassium permanganate of attachment removal is removed with clear water rinsing;The dehydration is to be taken off using 60 mesh filter cloth tripod pendulum type batch centrifugals
Water.
Preferably, water mill slurrying in step (2), gained slurries cross 60 mesh sieves, and gained filtrate is after 200 mesh after sieving
Sieve;Using the alkaline fly slurry of dilute NaOH tune pH value to 8.0 in step (3).
Preferably, after being cooled in step (4) less than 40 DEG C, with chitosan solution adjust fly starch pH value to 4.8~
5.0.
Preferably, the chitosan solution described in step (4) is with the chitosan solution institute of 15% acetate dissolution 1.5%
;With 800 mesh filter cloth centrifugal filtrations after the layering.
Preferably, the micro-filtration described in step (4) is to carry out micro-filtration with hollow-fibre membrane;The two-stage ultrafiltration is use
The ultrafiltration membrance filter of 10000Da can be intercepted, use can intercept the ultrafiltration membrance filter of 2000Da to gained filtrate again, remove macromolecular complex
Matter, filter residue obtained by micro-filtration and filter residue merging treatment obtained by two-stage ultrafiltration, concentration or do not concentrate, low temperature drying, Flyblow albumen powder.
Preferably, the concentration described in step (5) concentrates at reduced pressure conditions for filtrate, and 58 DEG C of temperature control, is concentrated into
Be25 DEG C, terminate concentration, to obtain the final product.
Preferably, granular pattern activated carbon is added at 60~70 DEG C with by the amount of concentrate mass fraction 2% in step (6)
Under the conditions of decolourize 2h, then with 600 mesh filter-cloth filterings, remove activated carbon.
Preferably, filtrate discharge is cooled to room temperature in step (7), sends to refrigerator-freezer or freezer is refrigerated to -37 DEG C, and keep
1-2h;Drying temperature described in step (8) is 58 DEG C, dry to be less than 5~6% to moisture content, terminates drying process.
Compared with prior art, the present invention has the advantages that:
The method of the present invention operation is succinct, and the extraction gained native peptides rate of recovery is up to 77.33%, substantially increases native peptides
The rate of recovery;Further, multiple byproducts can be prepared at the same time for the method for the present invention, suitable for big industrialized production, flow
Succinctly, it is easy to operate.
Brief description of the drawings
Fig. 1 is the method for the present invention flow chart.
Embodiment
Embodiment is described in detail below in conjunction with the accompanying drawings, it is to be understood that protection scope of the present invention and from
The limitation of embodiment.The NaOH solution that the dilute NaOH used in embodiment is 5~8% for mass concentration.
Embodiment 1
A kind of method that native peptides are extracted from fly, operating procedure are as follows:
(1) the chilled housefly for selecting 20kg freezen protectives is raw material, and wherein 13kg is the kind eliminated within production 4 generation of fly maggot
Fly, 7kg are the wild fly of trapping, and fly body surface is picked up with 3.5 ‰ liquor potassic permanganate of mass concentration rinsing 12min, then uses
Then clear water rinsing is dehydrated to body surface no moisture except the potassium permanganate of attachment removal using 60 mesh filter cloth tripod pendulum type batch centrifugals;
(2) gained fly after step (1) dehydration, by solid-liquid mass ratio 1:4 addition deionized waters grind slurry, and gained slurries are used
Tripod pendulum type batch centrifugal crosses 60 mesh sieves, and tripod pendulum type batch centrifugal crosses 200 mesh filter clothes to gained filtrate again after sieving, and the drying of gained filter residue is capable of using as feed
The raw material of additive or chitin processed;
(3) gained filtrate using the alkaline fly slurry of dilute NaOH tune pH value to 8.0, is placed in after the filtering of 200 mesh in step (2)
60 DEG C of microwave treatment 27min of temperature control in the microwave tank that microwave frequency is 2450MHZ, 40min is kept the temperature between 55~60 DEG C;
(4) after the fly slurry discharge cooling that microwave treatment is crossed in step (3) being less than 40 DEG C, with 15% acetate dissolution 1.5%
Chitosan solution adjust pH value to 4.8~5.0, precipitating protein, polysaccharide, fat etc., stand, with the filter of 800 mesh after being layered
Cloth tripod pendulum type batch centrifugal centrifugal filtration, filter residue low temperature drying, obtains fly albumen powder;Filtrate carries out micro-filtration successively, two-stage ultrafiltration removes
Macromolecular substances, i.e., carry out micro-filtration, first use can intercept the ultrafiltration membrance filter (ultrafiltration of 10000Da to gained filtrate with hollow-fibre membrane
1), use can intercept the ultrafiltration membrance filter (ultrafiltration 2) of 2000Da to gained filtrate again, remove macromolecular substances, filter residue obtained by micro-filtration with
Filter residue merging treatment obtained by two-stage ultrafiltration, concentration or do not concentrate, low temperature drying, fly albumen powder;
(5) gained filtrate reverse osmosis membrane after ultrafiltration in step (4), i.e. nanofiltration filtering, filtrate is at the same time during nanofiltration
It can be concentrated, filter residue obtained by nanofiltration is low price salt, removes low price salt, and filtrate obtained by nanofiltration concentrates again at reduced pressure conditions, temperature control
58 DEG C, about Be25 DEG C is concentrated into, terminates concentration;
(6) gained concentrate in step (5) is discharged, granular pattern activity is added with by the amount of concentrate mass fraction 2%
Charcoal decolourizes 2h under the conditions of 65 DEG C, then with 600 mesh filter-cloth filterings, removes activated carbon;
(7) gained filtrate discharge after filtering in step (6) is cooled to room temperature, sends to refrigerator-freezer or freezer is refrigerated to -37 DEG C,
And keep 1.5h;
(8) gained ice cube takes out after when will be refrigerated to -37 DEG C and small holding 2, breaks into fritter and is done with microwave vacuum dryer
Dry, 58 DEG C of temperature control is dry to be less than 5% to moisture content, terminates drying process, loose shape product is obtained, by client's requirement powder
It is broken or do not crush and packed, it is fly native peptides.
Embodiment 2
A kind of method that native peptides are extracted from fly, operating procedure are as follows:
(1) the chilled housefly for selecting 20kg freezen protectives is raw material, and wherein 12kg is the kind eliminated within production 4 generation of fly maggot
Fly, 8kg are the big pipunculid of trapping, and fly body surface is picked up with 3.5 ‰ liquor potassic permanganate of mass concentration rinsing 12min, then uses
Then clear water rinsing is dehydrated to body surface no moisture except the potassium permanganate of attachment removal using 60 mesh filter cloth tripod pendulum type batch centrifugals;
(2) gained fly after step (1) dehydration, by solid-liquid mass ratio 1:4 addition deionized waters grind slurry, and gained slurries are used
Tripod pendulum type batch centrifugal crosses 60 mesh sieves, and tripod pendulum type batch centrifugal crosses 200 mesh filter clothes to gained filtrate again after sieving, and the drying of gained filter residue is capable of using as feed
The raw material of additive or chitin processed;
(3) gained filtrate using the alkaline fly slurry of dilute NaOH tune pH value to 8.0, is placed in after the filtering of 200 mesh in step (2)
60 DEG C of microwave treatment 25min of temperature control in the microwave tank that microwave frequency is 2450MHZ, 40min is kept the temperature between 55~60 DEG C;
(4) after the fly slurry discharge cooling that microwave treatment is crossed in step (3) being less than 40 DEG C, with 15% acetate dissolution 1.5%
Chitosan solution adjust pH value to 4.8~5.0, precipitating protein, polysaccharide, fat etc., stand, with the filter of 800 mesh after being layered
Cloth tripod pendulum type batch centrifugal centrifugal filtration, filter residue low temperature drying, obtains fly albumen powder;Filtrate carries out micro-filtration successively, two-stage ultrafiltration removes
Macromolecular substances, i.e., carry out micro-filtration, first use can intercept the ultrafiltration membrance filter (ultrafiltration of 10000Da to gained filtrate with hollow-fibre membrane
1), use can intercept the ultrafiltration membrance filter (ultrafiltration 2) of 2000Da to gained filtrate again, remove macromolecular substances, filter residue obtained by micro-filtration with
Filter residue merging treatment obtained by two-stage ultrafiltration, concentration or do not concentrate, low temperature drying, fly albumen powder;
(5) gained filtrate reverse osmosis membrane after ultrafiltration in step (4), i.e. nanofiltration filtering, filtrate is at the same time during nanofiltration
It can be concentrated, filter residue obtained by nanofiltration is low price salt, removes low price salt, and filtrate obtained by nanofiltration concentrates again at reduced pressure conditions, temperature control
58 DEG C, about Be25 DEG C is concentrated into, terminates concentration;
(6) gained concentrate in step (5) is discharged, granular pattern activity is added with by the amount of concentrate mass fraction 2%
Charcoal decolourizes 2h under the conditions of 60 DEG C, then with 600 mesh filter-cloth filterings, removes activated carbon;
(7) gained filtrate discharge after filtering in step (6) is cooled to room temperature, sends to refrigerator-freezer or freezer is refrigerated to -37 DEG C,
And keep 1h;
(8) gained ice cube takes out after when will be refrigerated to -37 DEG C and small holding 1, breaks into fritter and is done with microwave vacuum dryer
Dry, 58 DEG C of temperature control is dry to be less than 5% to moisture content, terminates drying process, loose shape product is obtained, by client's requirement powder
It is broken or do not crush and packed, it is fly native peptides.
Embodiment 3
A kind of method that native peptides are extracted from fly, operating procedure are as follows:
(1) the chilled housefly for selecting 20kg freezen protectives is raw material, and wherein 13kg is the kind eliminated within production 4 generation of fly maggot
Fly, 7kg are the wild fly of trapping, and fly body surface is picked up with 3.5 ‰ liquor potassic permanganate of mass concentration rinsing 12min, then uses
Then clear water rinsing is dehydrated to body surface no moisture except the potassium permanganate of attachment removal using 60 mesh filter cloth tripod pendulum type batch centrifugals;
(2) gained fly after step (1) dehydration, by solid-liquid mass ratio 1:4 addition deionized waters grind slurry, and gained slurries are used
Tripod pendulum type batch centrifugal crosses 60 mesh sieves, and tripod pendulum type batch centrifugal crosses 200 mesh filter clothes to gained filtrate again after sieving, and the drying of gained filter residue is capable of using as feed
The raw material of additive or chitin processed;
(3) gained filtrate using the alkaline fly slurry of dilute NaOH tune pH value to 8.0, is placed in after the filtering of 200 mesh in step (2)
60 DEG C of microwave treatment 30min of temperature control in the microwave tank that microwave frequency is 2450MHZ, 40min is kept the temperature between 55~60 DEG C;
(4) after the fly slurry discharge cooling that microwave treatment is crossed in step (3) being less than 40 DEG C, with 15% acetate dissolution 1.5%
Chitosan solution adjust pH value to 4.8~5.0, precipitating protein, polysaccharide, fat etc., stand, with the filter of 800 mesh after being layered
Cloth tripod pendulum type batch centrifugal centrifugal filtration, filter residue low temperature drying, obtains fly albumen powder;Filtrate carries out micro-filtration successively, two-stage ultrafiltration removes
Macromolecular substances, i.e., carry out micro-filtration, first use can intercept the ultrafiltration membrance filter (ultrafiltration of 10000Da to gained filtrate with hollow-fibre membrane
1), use can intercept the ultrafiltration membrance filter (ultrafiltration 2) of 2000Da to gained filtrate again, remove macromolecular substances, filter residue obtained by micro-filtration with
Filter residue merging treatment obtained by two-stage ultrafiltration, concentration or do not concentrate, low temperature drying, fly albumen powder;
(5) gained filtrate reverse osmosis membrane after ultrafiltration in step (4), i.e. nanofiltration filtering, filtrate is at the same time during nanofiltration
It can be concentrated, filter residue obtained by nanofiltration is low price salt, removes low price salt, and filtrate obtained by nanofiltration concentrates again at reduced pressure conditions, temperature control
58 DEG C, about Be25 DEG C is concentrated into, terminates concentration;
(6) gained concentrate in step (5) is discharged, granular pattern activity is added with by the amount of concentrate mass fraction 2%
Charcoal decolourizes 2h under the conditions of 70 DEG C, then with 600 mesh filter-cloth filterings, removes activated carbon;
(7) gained filtrate discharge after filtering in step (6) is cooled to room temperature, sends to refrigerator-freezer or freezer is refrigerated to -37 DEG C,
And keep 2h;
(8) gained ice cube takes out after when will be refrigerated to -37 DEG C and small holding 1, breaks into fritter and is done with microwave vacuum dryer
Dry, 58 DEG C of temperature control is dry to be less than 6% to moisture content, terminates drying process, loose shape product is obtained, by client's requirement powder
It is broken or do not crush and packed, it is fly native peptides.
Raw material fly contains native peptides 3.89% in embodiment 1, obtains the natural peptide product 817.40g of fly, peptide content
73.61%, the native peptides rate of recovery 77.38%.Peptide of the molecular weight less than 833Da that be consistent is not detected in product does not have detection to be more than yet
The peptide of 1800Da.Account for the 87.04% of total peptide amount from the peptide amount of 8 peptides to 14 peptides, accounted in this peptide fragment peptide amount it is most be 10 peptides and 11
Peptide, 10 peptides have 11 amino acid sequences, account for the 41.05% of total peptide amount, sequence YDESGPGIVH accounts for the 31.35% of total peptide;11 peptides
There are 8 amino acid sequences, account for the 23.61% of total peptide amount, sequence VGDDLTVTNPK accounts for the 20.17% of total peptide.
The effective ways that native peptides are extracted from fly can be drawn by invention, and the natural peptide content of gained is higher, it is main
Want peptide fragment content difference obvious, 10 peptides, 11 peptide contents are more, and orientable this two peptide fragment that extracts is utilized.
It is foregoing to the present invention specific exemplary embodiment description be in order to illustrate and illustration purpose.These descriptions
It is not wishing to limit the invention to disclosed precise forms, and it will be apparent that according to the above instruction, can be much changed
And change.The purpose of selecting and describing the exemplary embodiment is that explain that the certain principles of the present invention and its reality should
With so that those skilled in the art can realize and utilize the present invention a variety of exemplaries and
Various chooses and changes.The scope of the present invention is intended to be limited by claims and its equivalents.
Claims (10)
- A kind of 1. method that native peptides are extracted from fly, it is characterised in that include following operating procedure:(1) fly body surface is sterilized and be dehydrated to body surface no moisture;(2) gained fly after step (1) dehydration, by solid-liquid mass ratio 1:4 add water mill slurrying, the sieving of gained slurries;(3) gained filtrate adjusting pH value is starched into alkaline fly after sieving in step (2), 60 DEG C of 25~30min of microwave treatment of temperature control, 40min is kept the temperature between 55~60 DEG C;(4) by the fly slurry discharge cooling that microwave treatment is crossed in step (3), pH value is adjusted to 4.8~5.0, is stood, the mistake after being layered Filter, filtrate carry out micro-filtration, two-stage ultrafiltration successively;(5) gained filtrate reverse osmosis membrane, i.e. nanofiltration are filtered after ultrafiltration in step (4), the concentration of gained filtrate;(6) gained concentrate after concentration in step (5) is discharged, adds activated carbon decolorizing, filtering, removes activated carbon;(7) gained filtrate discharge after filtering in step (6) is cooled to room temperature;(8) gained ice cube takes out after when will be refrigerated to -37 DEG C and small holding 1~2, dry, up to product.
- 2. according to the method described in claim 1, it is characterized in that:Fly described in step (1) for trapping wild housefly or At least one of Hylemyia Platura Meigen that maggot is eliminated is educated in big pipunculid, or artificial feeding.
- 3. according to the method described in claim 1, it is characterized in that:Disinfection described in step (1) is fly fly body quality 3.5 ‰ liquor potassic permanganate of concentration rinsing 12min is picked up, then is rinsed with water;The dehydration be using 60 mesh filter cloth tripodias from Scheming is dehydrated.
- 4. according to the method described in claim 1, it is characterized in that:Water mill slurrying in step (2), gained slurries cross 60 mesh sieves, Gained filtrate is after 200 mesh sieves after sieving;Using the alkaline fly slurry of dilute NaOH tune pH value to 8.0 in step (3).
- 5. according to the method described in claim 1, it is characterized in that:It is molten with chitosan after being cooled in step (4) less than 40 DEG C Liquid adjusts fly and starches pH value to 4.8~5.0.
- 6. according to the method described in claim 1, it is characterized in that:Chitosan solution described in step (4) is with 15% vinegar Obtained by the chitosan solution of acid dissolving 1.5%;With 800 mesh filter cloth centrifugal filtrations after the layering.
- 7. according to the method described in claim 1, it is characterized in that:Micro-filtration described in step (4) be with hollow-fibre membrane into Row micro-filtration;The two-stage ultrafiltration can intercept the ultrafiltration membrance filter of 10000Da for use, and use can intercept 2000Da's to gained filtrate again Ultrafiltration membrance filter, filter residue obtained by micro-filtration and filter residue merging treatment obtained by two-stage ultrafiltration.
- 8. according to the method described in claim 1, it is characterized in that:Concentration described in step (5) is filtrate in reduced pressure Lower concentration, 58 DEG C of temperature control, is concentrated into Be25 DEG C, terminates concentration, to obtain the final product.
- 9. according to the method described in claim 1, it is characterized in that:Added in step (6) with the amount by concentrate mass fraction 2% Enter activated carbon to decolourize under the conditions of 60~70 DEG C 2h, then with 600 mesh filter-cloth filterings, remove activated carbon.
- 10. according to the method described in claim 1, it is characterized in that:Filtrate discharge is cooled to room temperature in step (7), be refrigerated to- 37 DEG C, and keep 1-2h;Drying temperature described in step (8) is 58 DEG C, and drying to moisture content is less than 5~6%.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201711140091.9A CN107903300A (en) | 2017-11-16 | 2017-11-16 | A kind of method that native peptides are extracted from fly |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201711140091.9A CN107903300A (en) | 2017-11-16 | 2017-11-16 | A kind of method that native peptides are extracted from fly |
Publications (1)
Publication Number | Publication Date |
---|---|
CN107903300A true CN107903300A (en) | 2018-04-13 |
Family
ID=61845871
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201711140091.9A Pending CN107903300A (en) | 2017-11-16 | 2017-11-16 | A kind of method that native peptides are extracted from fly |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN107903300A (en) |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101054408A (en) * | 2007-04-02 | 2007-10-17 | 贵阳医学院 | Method for separating housefly secretion type antibacterial peptide, product and application thereof |
CN101328207A (en) * | 2008-05-08 | 2008-12-24 | 华南农业大学 | Citrus fruit fly antibacterial peptide, preparation and use thereof |
CN102321164A (en) * | 2011-05-25 | 2012-01-18 | 绍兴市曙光科技开发有限公司 | Preparation method and application of insect natural antibacterial peptide products |
CN103555798A (en) * | 2013-10-25 | 2014-02-05 | 江苏久吾高科技股份有限公司 | Method for extracting protein of fly maggot through ceramic membrane |
CN106892961A (en) * | 2017-02-14 | 2017-06-27 | 广西肽王生物科技有限公司 | A kind of separating and extracting process of fly-maggot protein polypeptide and fly maggot oil |
-
2017
- 2017-11-16 CN CN201711140091.9A patent/CN107903300A/en active Pending
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101054408A (en) * | 2007-04-02 | 2007-10-17 | 贵阳医学院 | Method for separating housefly secretion type antibacterial peptide, product and application thereof |
CN101328207A (en) * | 2008-05-08 | 2008-12-24 | 华南农业大学 | Citrus fruit fly antibacterial peptide, preparation and use thereof |
CN102321164A (en) * | 2011-05-25 | 2012-01-18 | 绍兴市曙光科技开发有限公司 | Preparation method and application of insect natural antibacterial peptide products |
CN103555798A (en) * | 2013-10-25 | 2014-02-05 | 江苏久吾高科技股份有限公司 | Method for extracting protein of fly maggot through ceramic membrane |
CN106892961A (en) * | 2017-02-14 | 2017-06-27 | 广西肽王生物科技有限公司 | A kind of separating and extracting process of fly-maggot protein polypeptide and fly maggot oil |
Non-Patent Citations (1)
Title |
---|
HUAN ZHANG等: "Antioxidant activities of protein hydrolysates obtained from the houseely larvae", 《ACTA BIOLOGICA HUNGARICA》 * |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN106282287B (en) | Method for extracting bioactive polypeptide of ginkgo | |
Junianto et al. | The influence of concentration of acetic acid and pepsin enzyme in nilem fish skin collagen extractionto the amount of rendement Produced | |
WO2015010497A1 (en) | Method for preparing lycium ruthenicum polysaccharide | |
CN105132506A (en) | Preparation method of chili seed antibacterial peptide | |
CN101731615A (en) | Preparation method of clam nutrition health care condiment | |
CN101314621A (en) | Squash polysaccharide and preparation method thereof | |
CN107815483A (en) | The method that mussel oligopeptide is prepared using mussel fresh meat | |
CN110760509A (en) | Method for extracting milt component of globefish testis | |
CN102150855B (en) | Preparation method for fresh anoectochilus roxburghii juice, and oral liquid, capsules and dissolved granules | |
CN102048026B (en) | Preparation method of oil-tea camellia meal protein polypeptide as additive for functional feeds | |
CN106359839A (en) | Extraction method of oyster peptides | |
CN103039937A (en) | Plant salt and preparation method thereof | |
CN104611310A (en) | Technology for extracting proteins and superoxide dismutase (SOD) from horseradish tree leaves | |
CN101965897B (en) | Processing method for mussel isolated protein | |
CN107805273A (en) | A kind of method that native peptides are extracted from fly maggot | |
CN109402073A (en) | The method for integrated extraction of various bioactive components in a kind of garlic | |
CN102517364B (en) | Method for preparing angiotensin converting enzyme (ACE) inhibitory peptide from sea cucumber | |
CN106119328A (en) | A kind of high-quality shrimp oligopeptide powder, preparation method thereof of applicable industrialized production | |
CN103951737B (en) | A kind of method that close glycoprotein is extracted from marine alga | |
CN107903300A (en) | A kind of method that native peptides are extracted from fly | |
CN101461513A (en) | Method for preparing water-soluble cochinilin color lake | |
CN100352839C (en) | Method for preparing insect protein with blood-fat reducing function | |
CN1130979C (en) | Process for preparing tea product and its product | |
CN107641160A (en) | Utilize the method for multi-stage countercurrent enzymolysis coupling technology extraction sea grass polysaccharide | |
CN105018557A (en) | Preparation method of pumpkin seed meal small peptides |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
RJ01 | Rejection of invention patent application after publication | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180413 |