CN107805273A - A kind of method that native peptides are extracted from fly maggot - Google Patents
A kind of method that native peptides are extracted from fly maggot Download PDFInfo
- Publication number
- CN107805273A CN107805273A CN201711140074.5A CN201711140074A CN107805273A CN 107805273 A CN107805273 A CN 107805273A CN 201711140074 A CN201711140074 A CN 201711140074A CN 107805273 A CN107805273 A CN 107805273A
- Authority
- CN
- China
- Prior art keywords
- maggot
- gained
- fly maggot
- fly
- value
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention discloses a kind of method that native peptides are extracted from fly maggot, operating procedure is that fly maggot body surface is sterilized and be dehydrated to body surface no moisture by (1)；(2) plus water mill is into slurry, sieving, then adds sodium hydrogensulfite and vitamin C, produces maggot slurry；(3) pH value, microwave treatment, insulation are adjusted；(4) filter, gained filter residue can make the raw material of chitin, gained filtrate regulation pH value, stand, filtered after being layered, filtrate carries out micro-filtration, two-stage ultrafiltration；(5) filtrate reverse osmosis membrane, i.e. nanofiltration filter；(6) concentrate discharge cooling, freezing；(7) freeze, dry, produce product.The inventive method operation is succinct, and the extraction gained native peptides rate of recovery is up to 77.33%, substantially increases the rate of recovery of native peptides；Further, multiple byproducts can be prepared simultaneously for the inventive method, and suitable for big industrialized production, flow is succinct, easy to operate.
Description
Technical field
The present invention relates to the extracting method of native peptides, more particularly to a kind of method that native peptides are extracted from fly maggot.
Background technology
According to the relevant information, known caste reaches more than 80 ten thousand kinds in the world, it is all dynamic equivalent on the earth
The 3/5 of species and the 4/5 of whole terrestrial animal gross weight.Insect is adaptable, widely distributed, its living space day flaring
Greatly, it is the living resources not being fully utilized maximum on the earth.The insect that the mankind utilize earliest is silkworm and honeybee, is had several
The history of thousand.In the past 50 years, the focus of insect utilization research is the housefly maggot and major part fly maggot in flies, and this is due to housefly
Maggot and major part fly maggot have the characteristics of other insects do not have, and are characterized in：1) breeding is fast, the cycle is short, yield is high；Just hatch
Small grub, every only 0.07~0.09mg is ripe through the cultivation of 4~5 days, reaches every 20~25mg, its total biomass
Increase by 220~350 times, be so far with other methods production animality yield it is incomparable；2) feeding habits are miscellaneous, source is wide, valency
It is honest and clean, it can especially utilize the pollution sources of resourcebility；3) premunition is strong, maggot body is interior contains extractable more bioactive substance.
The fly maggot breeding industry in China, to solve the shortage of the animal protein feed of poultry fish, starts research and production is high the eighties in last century
Tide, constantly find under study for action in addition to it largely can produce high-quality protein feed, moreover it is possible to obtain through refining the protein for human consumption, health care
The multiple product such as product and medicine.
In fly maggot breeding, no matter with the leftover bits and pieces of various agricultural byproducts processings or putrid and deteriorated food or the excreta of poultry
Compost is done, these composts all contain largely to the bacterium of human hazard, virus, microorganism etc., but fly maggot can be therefrom
Draw useful nutrition and normal growth and development, and never contaminate any disease.This phenomenon, can effectively it illustrate, fly
Maggot has the disease-resistant gene of fly and the defense system itself set up, and has the biology of resistance multiple pathogenic microorganisms living
Property material.
It is reported that true wait of fourth friend isolates a kind of energy strong inhibition staphylococcus aureus, Pseudomonas aeruginosa out of fly maggot body
Alcaligenes faecalis, the mechanism of wound can be treated by disclosing fly maggot slurry or fly maggot powder, and the tissue homogenate of fly maggot has antiviral work
With the inhibiting rate of, 15 microlitres of concentration infected by influenza up to more than 72.4%, 30 microlitre of concentration inhibiting rate up to more than 80%, and
87.5% is reached to the destructive rate of hepatitis b virus s antigen.Nankai University's Life Science College and University Of Tianjin's materialogy
Institute researcher cooperates, and isolates serial antibacterial peptide out of maggot slurry, can efficiently kill a variety of pathogens.Obtain the studies above achievement
The enlightenment of report, it is believed that in fly maggot body in addition to it above-mentioned bioactive substance be present, it is also possible to have more bioactive substances.
Then detection research is carried out to fly maggot, discovery contains native peptides, the content of native peptides and the growing environment of fly maggot, compost
Matter, fly species are wild to have larger difference with relevant, the different growth conditions of factor, amounts containing native peptides such as artificial farming.Fresh fly
Natural peptide content from 0.3%-4.0%, in the detection sample of collection with the natural peptide content of wild Chrysomya megacephala maggot most
Height, reach 4.2%, minimum content is bred and raised through 6 generations with wheat skin, the housefly maggot of dregs of beans, only 0.31%.But housefly maggot
Or major part fly maggot is raised with different feeds and different temperature, it is peptide fragment distribution that the natural polypeptides contained by them, which has a common feature,
Relatively, 2 peptides to 7 peptides less than 8 peptides do not have, more than yet not having for 20 peptides, the peptide amounts of 8 peptides to 14 peptides account for total peptide amount 80% with
On.These data extract the reference that native peptides are highly profitable to formulation from fly maggot.
Extraction native peptides extract from fly maggot mostly in the prior art, and extracting method is complicated, cost is high, and extract gained day
Right peptide yield is not high, therefore, needs a kind of extraction native peptides convenience, the succinct, method of high income from fly maggot badly at present.
The information for being disclosed in the background section is merely intended to understanding of the increase to the general background of the present invention, without answering
It has been the prior art well known to persons skilled in the art when being considered as recognizing or implying the information structure in any form.
The content of the invention
The present invention is existing so as to solve in order to solve the above technical problems, a kind of method that native peptides are extracted from fly maggot of invention
There is the problem of extracting method complex operation in technology, cost are high, extraction native peptides yield is low.
To achieve the above object, technical scheme provided by the invention is as follows：
A kind of method that native peptides are extracted from fly maggot, includes following operating procedure：
(1) fly maggot body surface is sterilized and is dehydrated to body surface no moisture；
(2) gained fly maggot adds water mill sieving, then to add sodium hydrogensulfite and vitamin C into slurry after step (1) dehydration
(VC) maggot slurry, is produced；
(3) gained maggot slurry regulation pH value is starched into alkaline maggot in step (2), 58 DEG C of 25~30min of microwave treatment of temperature control, then
30min is incubated between 50~58 DEG C；
(4) maggot that microwave treatment is crossed in step (3) is starched into discharge, filtering, gained filter residue can make the raw material of chitin, institute
Obtain filtrate and adjust pH value to 4.8~5.0, precipitating protein, polysaccharide, fat etc., stand, filtered after being layered, filter residue low temperature is done
It is dry, obtain Flyblow albumen powder；Filtrate carries out micro-filtration successively, two-stage ultrafiltration removes macromolecular substances；
(5) gained filtrate reverse osmosis membrane, i.e. nanofiltration are filtered after ultrafiltration in step (4), and gained filter residue is low price salt, is removed
Low price salt, the concentration of gained filtrate；
(6) discharge of gained concentrate is cooled to room temperature after step (5) is concentrated, and sends to refrigerator-freezer or freezer freezing；
(7) gained ice cube takes out after step (6) being refrigerated into -35 DEG C and 1~2h of holding, breaks into fritter and is done with microwave vacuum
Dry machine is dried, and produces product.
Preferably, the fly maggot described in step (1) is to draw the fly maggot of wild fly blow hatching incubation and/or draw wild fly kind
Make raw material within four generations and with the fly maggot of non-clean Feed Manufacturing.
Preferably, the sterilization described in step (1) is that the liquor potassic permanganate of fly maggot mass concentration 3 ‰ rinses 10min
Pick up, then the liquor potassic permanganate of attachment removal is removed with clear water rinsing；Described dehydration is to be taken off using 60 mesh filter cloth tripod pendulum type batch centrifugals
Water.
Preferably, the sodium hydrogensulfite added in step (2) is the 3.5 ‰ of the fly maggot weight added before defibrination, is added
VC be fly maggot weight 1 ‰.
Preferably, gained fly maggot presses solid-liquid weight than 1 after being dehydrated in step (2)：3 addition deionized waters wear into slurry, institute
Obtain slurries and cross 60 mesh sieves, then add sodium hydrogensulfite and Vc.
Preferably, the pH of maggot slurry after detecting microwave treatment during insulation 30min between 50~58 DEG C in step (3)
Value, as pH value ＜ 7.5, terminate microwave treatment process, the microwave treatment 15min again as pH value ＞ 7.5, be incubated 20min, terminate
Microwave treatment process；The alkaline maggot slurry of pH value to 8.0 is adjusted in step (3) using dilute NaOH.
Preferably, pH value is adjusted using with the chitosan solution of 15% acetate dissolution 1.5% in step (4)；Step (4)
The maggot slurry discharge that the microwave treatment is crossed, is filtered using 400 mesh filter cloth tripod pendulum type batch centrifugals；With 600 mesh after step (4) described layering
Filter cloth centrifugal filtration.
Preferably, the micro-filtration described in step (4) is to carry out micro-filtration with hollow-fibre membrane；Described two-stage ultrafiltration is use
10000Da ultrafiltration membrance filter can be intercepted, use can intercept 2000Da ultrafiltration membrance filter to gained filtrate again, remove macromolecular complex
Matter, filter residue obtained by micro-filtration and filter residue merging treatment obtained by two-stage ultrafiltration, concentration or do not concentrate, low temperature drying, Flyblow albumen powder.
Preferably, the concentration described in step (5) concentrates for filtrate, and gained concentrate is dense under 58 DEG C of reduced pressures
Contracting, Be25 DEG C is concentrated into, terminates concentration, produce；Step (5) the concentrate discharge is cooled to room temperature, sends to refrigerator-freezer or freezer
- 35 DEG C are refrigerated to, and keeps 1~2h.
Preferably, the drying temperature described in step (7) is 58 DEG C, dries to moisture content and is less than 5~6%, is terminated dry
Drying process.
Compared with prior art, the present invention has the advantages that：
The inventive method operation is succinct, and the extraction gained native peptides rate of recovery is up to 77.33%, substantially increases native peptides
The rate of recovery；Further, multiple byproducts can be prepared simultaneously for the inventive method, suitable for big industrialized production, flow
Succinctly, it is easy to operate.
Brief description of the drawings
Fig. 1 is the inventive method flow chart.
Embodiment
Embodiment is described in detail below in conjunction with the accompanying drawings, it is to be understood that protection scope of the present invention not by
The limitation of embodiment.The sodium hydroxide solution that the dilute NaOH used in embodiment is 5~8% for mass concentration.
Embodiment 1
A kind of method that native peptides are extracted from fly maggot, includes following operating procedure：
(1) 65% is accounted for from leftover bits and pieces of the compost for market of farm produce fish and chicken and duck processing, wheat skin accounts for the fly of 35% culture
Maggot 30kg, wherein 22kg propagate the 4th generation maggot artificially, and 8kg is to draw the fly maggot that wild fly spawning and hatching is cultivated to make raw material, by fly maggot body
Table is picked up with the liquor potassic permanganate of mass concentration 3 ‰ rinsing 10min, then the liquor potassic permanganate of attachment removal is removed with clear water rinsing,
Then it is dehydrated using 60 mesh filter cloth tripod pendulum type batch centrifugals to body surface no moisture；
(2) gained fly maggot presses solid-liquid weight than 1 after step (1) dehydration：3 addition deionized waters wear into slurry, gained slurries mistake
60 mesh sieves, then 3.5 ‰ into gained filtrate after sieving add sodium hydrogensulfite according to the fly maggot weight of addition and add
The 1 ‰ of fly maggot weight add VC, produce maggot slurry；
(3) gained maggot slurry adjusts the alkaline maggot slurry of pH value to 8.0 using dilute NaOH in step (2), and being placed in microwave frequency is
58 DEG C of microwave treatment 27min of temperature control in 2450MHZ microwave tank, 30min is then incubated between 50~58 DEG C, is detected at microwave
The pH value that maggot is starched after reason, as pH value ＜ 7.5, terminate microwave treatment process, the microwave treatment 15min again as pH value ＞ 7.5, protect
Warm 20min, terminate microwave treatment process；
(4) maggot that microwave treatment is crossed in step (3) is starched into discharge, filtered using 400 mesh filter cloth tripod pendulum type batch centrifugals, gained filter
Slag can make the raw material of chitin, gained filtrate with the chitosan solution of 15% acetate dissolution 1.5% adjust pH value to 4.8~
5.0, precipitating protein, polysaccharide, fat etc., stand, it is low with 600 mesh filter cloth tripod pendulum type batch centrifugal centrifugal filtrations, filter residue after being layered
Temperature is dried, and obtains Flyblow albumen powder；Filtrate carries out micro-filtration successively, two-stage ultrafiltration removes macromolecular substances, i.e., is entered with hollow-fibre membrane
Row micro-filtration, first use can intercept 10000Da ultrafiltration membrance filter to gained filtrate, and use can intercept 2000Da milipore filter to gained filtrate again
Filter, remove macromolecular substances, filter residue obtained by micro-filtration and filter residue merging treatment obtained by two-stage ultrafiltration, concentrate or do not concentrate, low temperature
Dry, obtain Flyblow albumen powder；
(5) gained filtrate reverse osmosis membrane, i.e. nanofiltration are filtered after ultrafiltration in step (4), and gained filter residue is low price salt, is removed
Low price salt, is then concentrated, and gained concentrate concentrates again at reduced pressure conditions after concentration, 58 DEG C of temperature control, is concentrated into about Be25 DEG C,
Terminate concentration；
(6) step (5) is concentrated into Be25 DEG C of concentrate discharge and is cooled to room temperature, send to refrigerator-freezer or freezer be refrigerated to-
35 DEG C, and keep 2h；
(7) step (6) is refrigerated to -35 DEG C and keeps gained ice cube after 1h to take out, break into fritter micro-wave vacuum
Machine is dried, 58 DEG C of temperature control, is dried to moisture content and is less than 5%, is terminated drying process, is obtained loose shape product, and being used by client will
Ask crushing or do not crush and packed, as fly maggot native peptides.
Embodiment 2
A kind of method that native peptides are extracted from fly maggot, includes following operating procedure：
(1) 65% is accounted for from leftover bits and pieces of the compost for market of farm produce fish and chicken and duck processing, wheat skin accounts for the fly of 35% culture
Maggot 30kg, wherein 30kg propagate the 4th generation maggot artificially and make raw material, and fly maggot body surface is rinsed with the liquor potassic permanganate of mass concentration 3 ‰
10min is picked up, then removes the liquor potassic permanganate of attachment removal with clear water rinsing, then using 60 mesh filter cloth tripod pendulum type batch centrifugals be dehydrated to
Body surface no moisture；
(2) gained fly maggot presses solid-liquid weight than 1 after step (1) dehydration：3 addition deionized waters wear into slurry, gained slurries mistake
60 mesh sieves, then 3.5 ‰ into gained filtrate after sieving add sodium hydrogensulfite according to the fly maggot weight of addition and add
The 1 ‰ of fly maggot weight add VC, produce maggot slurry；
(3) gained maggot slurry adjusts the alkaline maggot slurry of pH value to 8.0 using dilute NaOH in step (2), and being placed in microwave frequency is
58 DEG C of microwave treatment 25min of temperature control in 2450MHZ microwave tank, 30min is then incubated between 50~58 DEG C, is detected at microwave
The pH value that maggot is starched after reason, as pH value ＜ 7.5, terminate microwave treatment process, the microwave treatment 15min again as pH value ＞ 7.5, protect
Warm 20min, terminate microwave treatment process；
(4) maggot that microwave treatment is crossed in step (3) is starched into discharge, filtered using 400 mesh filter cloth tripod pendulum type batch centrifugals, gained filter
Slag can make the raw material of chitin, gained filtrate with the chitosan solution of 15% acetate dissolution 1.5% adjust pH value to 4.8~
5.0, precipitating protein, polysaccharide, fat etc., stand, it is low with 600 mesh filter cloth tripod pendulum type batch centrifugal centrifugal filtrations, filter residue after being layered
Temperature is dried, and obtains Flyblow albumen powder；Filtrate carries out micro-filtration successively, two-stage ultrafiltration removes macromolecular substances, i.e., is entered with hollow-fibre membrane
Row micro-filtration, first use can intercept 10000Da ultrafiltration membrance filter to gained filtrate, and use can intercept 2000Da milipore filter to gained filtrate again
Filter, remove macromolecular substances, filter residue obtained by micro-filtration and filter residue merging treatment obtained by two-stage ultrafiltration, concentrate or do not concentrate, low temperature
Dry, obtain Flyblow albumen powder；
(5) gained filtrate reverse osmosis membrane, i.e. nanofiltration are filtered after ultrafiltration in step (4), and gained filter residue is low price salt, is removed
Low price salt, is then concentrated, and gained concentrate concentrates again at reduced pressure conditions after concentration, 58 DEG C of temperature control, is concentrated into about Be25 DEG C,
Terminate concentration；
(6) step (5) is concentrated into Be25 DEG C of concentrate discharge and is cooled to room temperature, send to refrigerator-freezer or freezer be refrigerated to-
35 DEG C, and keep 1h；
(7) step (6) is refrigerated to -35 DEG C and keeps gained ice cube after 2h to take out, break into fritter micro-wave vacuum
Machine is dried, 58 DEG C of temperature control, is dried to moisture content and is less than 5%, is terminated drying process, is obtained loose shape product, and being used by client will
Ask crushing or do not crush and packed, as fly maggot native peptides.
Embodiment 3
A kind of method that native peptides are extracted from fly maggot, includes following operating procedure：
(1) 65% is accounted for from leftover bits and pieces of the compost for market of farm produce fish and chicken and duck processing, wheat skin accounts for the fly of 35% culture
Maggot 30kg, wherein 30kg are to draw the fly maggot that wild fly spawning and hatching is cultivated to make raw material, by the fly maggot body surface Gao Meng of mass concentration 3 ‰
Sour potassium solution rinsing 10min is picked up, then the liquor potassic permanganate of attachment removal is removed with clear water rinsing, then using 60 mesh filter cloth tripodias
Centrifuge dewatering is to body surface no moisture；
(2) gained fly maggot presses solid-liquid weight than 1 after step (1) dehydration：3 addition deionized waters wear into slurry, gained slurries mistake
60 mesh sieves, then 3.5 ‰ into gained filtrate after sieving add sodium hydrogensulfite according to the fly maggot weight of addition and add
The 1 ‰ of fly maggot weight add VC, produce maggot slurry；
(3) gained maggot slurry adjusts the alkaline maggot slurry of pH value to 8.0 using dilute NaOH in step (2), and being placed in microwave frequency is
58 DEG C of microwave treatment 30min of temperature control in 2450MHZ microwave tank, 30min is then incubated between 50~58 DEG C, is detected at microwave
The pH value that maggot is starched after reason, as pH value ＜ 7.5, terminate microwave treatment process, the microwave treatment 15min again as pH value ＞ 7.5, protect
Warm 20min, terminate microwave treatment process；
(4) maggot that microwave treatment is crossed in step (3) is starched into discharge, filtered using 400 mesh filter cloth tripod pendulum type batch centrifugals, gained filter
Slag can make the raw material of chitin, gained filtrate with the chitosan solution of 15% acetate dissolution 1.5% adjust pH value to 4.8~
5.0, precipitating protein, polysaccharide, fat etc., stand, it is low with 600 mesh filter cloth tripod pendulum type batch centrifugal centrifugal filtrations, filter residue after being layered
Temperature is dried, and obtains Flyblow albumen powder；Filtrate carries out micro-filtration successively, two-stage ultrafiltration removes macromolecular substances, i.e., is entered with hollow-fibre membrane
Row micro-filtration, first use can intercept 10000Da ultrafiltration membrance filter to gained filtrate, and use can intercept 2000Da milipore filter to gained filtrate again
Filter, remove macromolecular substances, filter residue obtained by micro-filtration and filter residue merging treatment obtained by two-stage ultrafiltration, concentrate or do not concentrate, low temperature
Dry, obtain Flyblow albumen powder；
(5) gained filtrate reverse osmosis membrane, i.e. nanofiltration are filtered after ultrafiltration in step (4), and gained filter residue is low price salt, is removed
Low price salt, is then concentrated, and gained concentrate concentrates again at reduced pressure conditions after concentration, 58 DEG C of temperature control, is concentrated into about Be25 DEG C,
Terminate concentration；
(6) step (5) is concentrated into Be25 DEG C of concentrate discharge and is cooled to room temperature, send to refrigerator-freezer or freezer be refrigerated to-
35 DEG C, and keep 2h；
(7) step (6) is refrigerated to -35 DEG C and keeps gained ice cube after 2h to take out, break into fritter micro-wave vacuum
Machine is dried, 58 DEG C of temperature control, is dried to moisture content and is less than 6%, is terminated drying process, is obtained loose shape product, and being used by client will
Ask crushing or do not crush and packed, as fly maggot native peptides.
Raw material maggot native peptides containing maggot are 3.39% in embodiment 1, obtain natural peptide product 1076.6g, peptide content 78.30%,
Recovery rate 82.89%, recovery rate is 2.81 ‰ based on natural peptide content, and peptide is mainly distributed on 8 peptides to 14 peptides, accounts for peptide total amount
89.9%, wherein 8 peptides have the sequence of 3 amino, peptide total amount 1.56% is accounted for, 9 peptides have 9 amino sequences to account for peptide total amount 6.78%,
10 peptides have 6 amino sequences, account for peptide total amount 40.9%, and 11 peptides have the sequence of 6 amino, account for peptide total amount 29.95%, and 12 peptides have 5
The sequence of individual amino, peptide total amount 8.20% is accounted for, 13 peptides have the sequence of 7 amino, account for peptide total amount 2.09%, and 14 peptides there are 6 amino
Acid sequence, account for peptide total amount 1.42 ‰.In 10 peptides and 11 peptide sequences for comparing concentration, it is total that the YDESGPGIVH sequences of 10 peptides account for peptide
The 33.42% of amount, the VGDDLTVTNPK sequences of 11 peptides account for the 26.28% of peptide amount.
By this experimental procedure, the effective ways that native peptides are extracted from fly maggot, and the natural peptide content of gained can be drawn
Higher, main peptide fragment content difference is obvious, and 10 peptides, 11 peptide contents are more, and orientable this two peptide fragment that extracts is utilized.
The heavy filtering gained filter residue of the inventive method acid, if make Flyblow albumen powder recovery, centrifugation slag be dehydrated as far as possible it is some more,
The moisture content of slag is set to be less than 55%, and low temperature (60 DEG C of ＜) is dried in time, can avoid brown stain.If filter residue prepares fly maggot day as enzymolysis
During right peptide, merge with the dope of micro-filtration ultrafiltration and digested, it is impossible to it is continuous when digesting in time, should will merging material to be placed in temperature low
Preserved in 7 DEG C of conditions, otherwise cannot get native peptides powder product, even if concentrate is refrigerated into -38 DEG C, also cannot get powdery production
Product.
The description of the foregoing specific illustrative embodiment to the present invention is to illustrate and the purpose of illustration.These descriptions
It is not wishing to limit the invention to disclosed precise forms, and it will be apparent that according to above-mentioned teaching, can be much changed
And change.The purpose of selecting and describing the exemplary embodiment is that explain that the certain principles of the present invention and its reality should
With so that those skilled in the art can realize and utilize the present invention a variety of exemplaries and
Various chooses and changes.The scope of the present invention is intended to be limited by claims and its equivalents.
Claims (10)
- A kind of 1. method that native peptides are extracted from fly maggot, it is characterised in that include following operating procedure：(1) fly maggot body surface is sterilized and is dehydrated to body surface no moisture；(2) gained fly maggot adds water mill sieving, then to add sodium hydrogensulfite and vitamin C into slurry, produce after step (1) dehydration Maggot is starched；(3) gained maggot slurry regulation pH value is starched into alkaline maggot in step (2), 58 DEG C of 25~30min of microwave treatment of temperature control, then 50 30min is incubated between~58 DEG C；(4) maggot that microwave treatment is crossed in step (3) is starched into discharge, filtering, gained filter residue can make the raw material of chitin, gained filter Liquid adjusts pH value to 4.8~5.0, stands, is filtered after being layered, filtrate carries out micro-filtration, two-stage ultrafiltration successively；(5) gained filtrate reverse osmosis membrane, i.e. nanofiltration are filtered after ultrafiltration in step (4), the concentration of gained filtrate；(6) the discharge cooling of gained concentrate, freezing after step (5) is concentrated；(7) gained ice cube takes out after step (6) being refrigerated into -35 DEG C and 1~2h of holding, dries, produces product.
- 2. according to the method for claim 1, it is characterised in that：Fly maggot described in step (1) is hatched to draw wild fly blow The fly maggot of incubation and/or draw within the wild generation of fly kind four and make raw material with the fly maggot of non-clean Feed Manufacturing.
- 3. according to the method for claim 1, it is characterised in that：Sterilization described in step (1) is fly maggot mass concentration 3 ‰ liquor potassic permanganates rinsing 10min is picked up, then with clear water rinsing solution；Described dehydration be using 60 mesh filter cloth tripodias from Scheming is dehydrated.
- 4. according to the method for claim 1, it is characterised in that：The sodium hydrogensulfite added in step (2) be before defibrination plus The 3.5 ‰ of the fly maggot weight entered, the VC of addition are the 1 ‰ of the weight of fly maggot.
- 5. according to the method for claim 1, it is characterised in that：Gained fly maggot presses solid-liquid weight ratio after being dehydrated in step (2) 1：3 addition water mills cross 60 mesh sieves into slurry, gained slurries, then add sodium hydrogensulfite and Vc.
- 6. according to the method for claim 1, it is characterised in that：It is incubated in step (3) between 50~58 DEG C during 30min The pH value that maggot is starched after detection microwave treatment, as pH value ＜ 7.5, terminate microwave treatment process, as pH value ＞ 7.5 again at microwave 15min is managed, is incubated 20min, terminates microwave treatment process；The alkaline maggot of pH value to 8.0 is adjusted in step (3) using dilute NaOH Slurry.
- 7. according to the method for claim 1, it is characterised in that：Using the shell with 15% acetate dissolution 1.5% in step (4) Glycan solution adjusts pH value；The maggot slurry discharge that step (4) described microwave treatment is crossed, is filtered using 400 mesh filter cloth tripod pendulum type batch centrifugals； With 600 mesh filter cloth centrifugal filtrations after step (4) described layering.
- 8. according to the method for claim 1, it is characterised in that：Micro-filtration described in step (4) is to be entered with hollow-fibre membrane Row micro-filtration；Described two-stage ultrafiltration is the ultrafiltration membrance filter that use can intercept 10000Da, and use can intercept 2000Da's to gained filtrate again Ultrafiltration membrance filter, filter residue obtained by micro-filtration and filter residue merging treatment obtained by two-stage ultrafiltration.
- 9. according to the method for claim 1, it is characterised in that：Concentration described in step (5) concentrates for filtrate, and gained is dense Contracting liquid concentrates under 58 DEG C of reduced pressures, is concentrated into Be25 DEG C, terminates concentration, produces；Step (5) the concentrate discharge is cold But, -35 DEG C are refrigerated to, and keeps 1~2h.
- 10. according to the method for claim 1, it is characterised in that：Drying temperature described in step (7) is 58 DEG C, is dried It is less than 5~6% to moisture content.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711140074.5A CN107805273A (en) | 2017-11-16 | 2017-11-16 | A kind of method that native peptides are extracted from fly maggot |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711140074.5A CN107805273A (en) | 2017-11-16 | 2017-11-16 | A kind of method that native peptides are extracted from fly maggot |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107805273A true CN107805273A (en) | 2018-03-16 |
Family
ID=61580768
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711140074.5A Pending CN107805273A (en) | 2017-11-16 | 2017-11-16 | A kind of method that native peptides are extracted from fly maggot |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107805273A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108623653A (en) * | 2018-04-27 | 2018-10-09 | 罗乌支 | A method of it extracting antler native peptides from antler and enzymolysis prepares polypeptide |
| CN111662945A (en) * | 2020-07-13 | 2020-09-15 | 广西赣华生物科技有限公司 | Method for extracting noni peptide by microwave-assisted enzymolysis method and noni peptide powder |
| CN111748013A (en) * | 2020-07-13 | 2020-10-09 | 广西赣华生物科技有限公司 | Natural noni peptide extraction method and natural noni peptide powder |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104163847A (en) * | 2014-05-03 | 2014-11-26 | 吉林省金梓源生物科技有限公司 | Housefly larvae active protein peptide preparation method and housefly larvae active protein peptide prepared thereby and purpose of housefly larvae active protein peptide |
| CN105385739A (en) * | 2015-12-09 | 2016-03-09 | 梁尚文 | Method for extracting protein peptide from poecilobdella manillensis |
| CN107307173A (en) * | 2017-07-10 | 2017-11-03 | 罗乌支 | A kind of method that active protein powder is prepared with housefly maggot |
-
2017
- 2017-11-16 CN CN201711140074.5A patent/CN107805273A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104163847A (en) * | 2014-05-03 | 2014-11-26 | 吉林省金梓源生物科技有限公司 | Housefly larvae active protein peptide preparation method and housefly larvae active protein peptide prepared thereby and purpose of housefly larvae active protein peptide |
| CN105385739A (en) * | 2015-12-09 | 2016-03-09 | 梁尚文 | Method for extracting protein peptide from poecilobdella manillensis |
| CN107307173A (en) * | 2017-07-10 | 2017-11-03 | 罗乌支 | A kind of method that active protein powder is prepared with housefly maggot |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108623653A (en) * | 2018-04-27 | 2018-10-09 | 罗乌支 | A method of it extracting antler native peptides from antler and enzymolysis prepares polypeptide |
| CN111662945A (en) * | 2020-07-13 | 2020-09-15 | 广西赣华生物科技有限公司 | Method for extracting noni peptide by microwave-assisted enzymolysis method and noni peptide powder |
| CN111748013A (en) * | 2020-07-13 | 2020-10-09 | 广西赣华生物科技有限公司 | Natural noni peptide extraction method and natural noni peptide powder |
| CN111662945B (en) * | 2020-07-13 | 2023-08-11 | 广西赣华生物科技有限公司 | Method for extracting noni peptide by microwave-assisted enzymolysis and noni peptide powder |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR101889982B1 (en) | Method for preparing pet animal feed using larva of insect by feeding medicinal herb residues | |
| CN101948533B (en) | Preparation method of fish skin collagen | |
| CN104892037A (en) | Method for producing water-soluble fish protein organic fertilizer from fish | |
| CN107805273A (en) | A kind of method that native peptides are extracted from fly maggot | |
| CN101240313B (en) | Method for preparing scale collagen peptide | |
| Allan et al. | A critical evaluation of the potential sources of chitin and chitosan | |
| CN107099472B (en) | Bacillus cereus and application thereof in feather degradation | |
| CN103478509A (en) | Production method of bacillus amyloliquefaciens antimicrobial lipopeptide and application thereof in prawn feed | |
| CN107307173A (en) | A kind of method that active protein powder is prepared with housefly maggot | |
| CN102048026B (en) | Preparation method of oil-tea camellia meal protein polypeptide as additive for functional feeds | |
| CN105506029A (en) | Method for preparing antifungal pectin oligosaccharide through citrus peel, application thereof and citrus preservation method | |
| RU2688315C1 (en) | Method of producing protein-lipid biomass by growing hermetia illucens larvae on cellulose-containing wastes | |
| CN106282283A (en) | A kind of microwave-assisted prepares the method for freshwater fish bones protein polypeptide | |
| CN1821266A (en) | Process for extracting bread worm protein | |
| CN106086138A (en) | A kind of method that microwave-assisted and membrane filtration prepare fresh water fin albumen oligopeptide | |
| CN104642712A (en) | Extracting method for snail protein powder | |
| CN102952841A (en) | Method for preparing hyriopsis cumingii meat enzymolysis protein solution | |
| CN103755396A (en) | Preparation method of earthworm composite liquid for promoting wheat yield increase | |
| CN102168063B (en) | Method for separation and primary culture of intestinal mucosal cells of fishes | |
| CN112195213A (en) | Method for extracting protein powder by cultivating hermetia illucens with food waste solids | |
| CN100352839C (en) | Method for preparing insect protein with blood-fat reducing function | |
| CN105053504A (en) | Method for manufacturing edible fly maggot active protein raw powder | |
| CN100384989C (en) | Collecting method for insect virus breed | |
| CN108484716A (en) | A kind of extracting method of grub antibacterial peptide and its application | |
| CN107641160A (en) | Utilize the method for multi-stage countercurrent enzymolysis coupling technology extraction sea grass polysaccharide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20180316 |
|
| WD01 | Invention patent application deemed withdrawn after publication |