CN111662945B - Method for extracting noni peptide by microwave-assisted enzymolysis and noni peptide powder - Google Patents
Method for extracting noni peptide by microwave-assisted enzymolysis and noni peptide powder Download PDFInfo
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- 244000131360 Morinda citrifolia Species 0.000 title claims abstract description 80
- 235000017524 noni Nutrition 0.000 title claims abstract description 79
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims abstract description 32
- 239000000843 powder Substances 0.000 title claims abstract description 12
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 35
- 238000003756 stirring Methods 0.000 claims abstract description 21
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 16
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000000706 filtrate Substances 0.000 claims abstract description 11
- 238000001728 nano-filtration Methods 0.000 claims abstract description 9
- DWAQJAXMDSEUJJ-UHFFFAOYSA-M Sodium bisulfite Chemical compound [Na+].OS([O-])=O DWAQJAXMDSEUJJ-UHFFFAOYSA-M 0.000 claims abstract description 8
- 235000010323 ascorbic acid Nutrition 0.000 claims abstract description 8
- 229960005070 ascorbic acid Drugs 0.000 claims abstract description 8
- 239000011668 ascorbic acid Substances 0.000 claims abstract description 8
- 238000001471 micro-filtration Methods 0.000 claims abstract description 8
- 235000010267 sodium hydrogen sulphite Nutrition 0.000 claims abstract description 8
- 108090000790 Enzymes Proteins 0.000 claims abstract description 7
- 102000004190 Enzymes Human genes 0.000 claims abstract description 7
- 229940088598 enzyme Drugs 0.000 claims abstract description 7
- 238000001914 filtration Methods 0.000 claims abstract description 7
- 238000010298 pulverizing process Methods 0.000 claims abstract description 7
- 108090000526 Papain Proteins 0.000 claims abstract description 6
- 239000004365 Protease Substances 0.000 claims abstract description 6
- 239000000084 colloidal system Substances 0.000 claims abstract description 6
- 238000000227 grinding Methods 0.000 claims abstract description 6
- 229940055729 papain Drugs 0.000 claims abstract description 6
- 235000019834 papain Nutrition 0.000 claims abstract description 6
- 238000002791 soaking Methods 0.000 claims abstract description 6
- 239000004289 sodium hydrogen sulphite Substances 0.000 claims abstract description 6
- 238000004108 freeze drying Methods 0.000 claims abstract description 4
- 239000012528 membrane Substances 0.000 claims description 23
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 10
- 239000002002 slurry Substances 0.000 claims description 10
- 239000002994 raw material Substances 0.000 claims description 8
- 238000010438 heat treatment Methods 0.000 claims description 7
- 230000000415 inactivating effect Effects 0.000 claims description 7
- 238000001816 cooling Methods 0.000 claims description 5
- 235000011869 dried fruits Nutrition 0.000 claims description 5
- 239000004744 fabric Substances 0.000 claims description 5
- 239000002245 particle Substances 0.000 claims description 2
- 238000000605 extraction Methods 0.000 abstract description 9
- 238000004519 manufacturing process Methods 0.000 abstract description 4
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- 238000009776 industrial production Methods 0.000 abstract description 3
- 239000004480 active ingredient Substances 0.000 abstract description 2
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- 150000001413 amino acids Chemical class 0.000 description 4
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- 241001573881 Corolla Species 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 229930013930 alkaloid Natural products 0.000 description 2
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- 238000001514 detection method Methods 0.000 description 2
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- 229920001282 polysaccharide Polymers 0.000 description 2
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- 235000019624 protein content Nutrition 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
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- 235000013343 vitamin Nutrition 0.000 description 2
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- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 229930000680 A04AD01 - Scopolamine Natural products 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000083547 Columella Species 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- STECJAGHUSJQJN-GAUPFVANSA-N Hyoscine Natural products C1([C@H](CO)C(=O)OC2C[C@@H]3N([C@H](C2)[C@@H]2[C@H]3O2)C)=CC=CC=C1 STECJAGHUSJQJN-GAUPFVANSA-N 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 208000001953 Hypotension Diseases 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- STECJAGHUSJQJN-UHFFFAOYSA-N N-Methyl-scopolamin Natural products C1C(C2C3O2)N(C)C3CC1OC(=O)C(CO)C1=CC=CC=C1 STECJAGHUSJQJN-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 208000037062 Polyps Diseases 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 241001107098 Rubiaceae Species 0.000 description 1
- 241000256008 Samia Species 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- 241000534473 Valvata Species 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
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- 230000004071 biological effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
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- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 235000013351 cheese Nutrition 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000013355 food flavoring agent Nutrition 0.000 description 1
- 230000036543 hypotension Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- STECJAGHUSJQJN-FWXGHANASA-N scopolamine Chemical compound C1([C@@H](CO)C(=O)O[C@H]2C[C@@H]3N([C@H](C2)[C@@H]2[C@H]3O2)C)=CC=CC=C1 STECJAGHUSJQJN-FWXGHANASA-N 0.000 description 1
- 229960002646 scopolamine Drugs 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 210000004916 vomit Anatomy 0.000 description 1
- 230000008673 vomiting Effects 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/10—Process efficiency
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- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
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Abstract
The invention discloses a method for extracting noni peptide by a microwave-assisted enzymolysis method and noni peptide powder, belonging to the technical field of peptide extraction, wherein the method comprises the following steps: pulverizing dried noni fruit material, adding water, adding sodium bisulphite and ascorbic acid, stirring and soaking, grinding the soaked material in a colloid mill, sequentially adding alkaline enzyme and papain in a microwave tank, stirring for microwave enzymolysis, centrifuging, filtering, collecting filtrate, sequentially performing microfiltration, ultrafiltration twice and nanofiltration to obtain noni fruit peptide powder, and freeze-drying noni fruit peptide powder. The extraction method has simple and convenient process, easy control, low cost and easy realization of industrial production. The microwave radiation is adopted for assisting enzymolysis, so that energy can be provided for an enzymolysis system, the temperature required by enzymolysis is maintained, the enzymolysis efficiency is greatly improved, the enzymolysis time is reduced, the thoroughness and accuracy of enzymolysis are ensured, a new way is provided for the industrialized production of noni active ingredients, and obvious economic benefits are achieved.
Description
Technical Field
The invention relates to a peptide extraction method and freeze-dried powder, in particular to a method for extracting noni peptide by a microwave-assisted enzymolysis method and noni peptide powder, and belongs to the technical field of peptide extraction.
Background
The beach Morinda citrifolia (academic name: morinda citrifolia L.) is a shrub to small arbor of the family Rubiaceae, morinda citrifolia, commonly known as Morinda citrifolia, up to 5 meters; the stems are straight, and the branches are nearly quadrangular. The leaves are alternately regenerated, the two ends of the leaves are tapered or sharp, the leaves are glossy and have no hair, the two sides of the leaf veins are convex, and the armpits below the leaf veins are densely covered with short hair bundles; the leaves are grown among the petioles without hair. Every other segment of the head inflorescence grows with leaves, flowers are majority, and no stem exists; the calyx pipes are adhered to each other, and the calyx eave is truncated; the corolla is white, funnel-shaped, split egg-shaped and needle-shaped, the throat of the corolla is born, the filament is about 3 mm long, the anther is inward, the columella is about equal to the crown tube, the ovary is sometimes sterile, each room has 1 ovule, the ovule is slightly flat, the stone fruit of the polyp is berry-shaped, oval, small in seed, flat, oblong and full-year flower fruit period.
Noni grows mainly in indonesia, valvata, kuke islands, fijia, samia, etc. near the south pacific islands and the equator, and is the most prominent in the field of creeks. The northern hemisphere has also grown in Hawaiian islands, philippines in southeast Asia, cambodia, laos, burma, and the main areas of production in China are Hainan islands, sisha islands, and Taiwan islands.
The noni fruit is called super fruit, the fruit is rich in 227 nutritional ingredients, wherein the noni fruit comprises 13 vitamins (A, B, C, E and the like), 16 mineral substances (potassium, sodium, zinc, calcium, iron, magnesium, phosphorus, copper, selenium and the like), 8 trace elements, more than 20 amino acids (super combination with most amino acid types in plants) which are necessary for 9 human bodies, more than 10 substances with antioxidant effect, more than one scopolamine and other various alkaloids, polysaccharides, various enzymes and other components with medical value, and the noni fruit is a very powerful natural comprehensive vitamin.
Noni fruits are rich in human body cell components and have the effect of strengthening body. It can promote digestion and absorption of food, and promote rapid recovery of physical strength. Noni also has physical constitution improving, antiinflammatory, antibacterial, hypotension or hypertension regulating, blood sugar regulating, and self-healing effects.
However, noni ripe fruits have strong odor, so that the noni ripe fruits are also called as 'cheese fruits' or 'vomit fruits', and the fruits are bad in taste, bitter and not preserved, so that most of people process the noni ripe fruits into fruit pulp, fruit juice or ferment, but a plurality of flavoring agents and preservatives are required to be added into the noni fruits to prepare the fruit juice and ferment, so that the noni ripe fruits are not beneficial to human health and are not easy to absorb by human bodies.
Through the research on the deep processing and utilization of noni fruits, the noni fruits are rich in natural peptides and various active substances beneficial to human bodies, and noni fruit polysaccharide alkaloids and the like can be carried until corresponding cell target organs generate biological effects, so that the extraction and preparation of noni fruit peptides are the direction of the deep processing and utilization of noni fruits.
Disclosure of Invention
In view of the above, the invention provides a method for extracting natural small molecular peptides from noni fruits, which adopts microwave enzymolysis technology to extract noni peptides from noni dried fruits, and can be widely used for the treatment and health care of sub-health people.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
a method for extracting noni peptides by a microwave-assisted enzymolysis method, which comprises the following steps:
1) Pulverizing dried noni fruit, adding water, adding sodium bisulphite and ascorbic acid, stirring and soaking for 10-15min;
2) Grinding the soaked raw materials in a colloid mill, and adjusting the pH value of the slurry to 8.5 at 10-50 ℃;
3) Placing the slurry in a microwave tank with power of 2-10kw, adding alkaline enzyme, stirring for microwave enzymolysis for 45-50min, adding papain, stirring for microwave enzymolysis for 45-50min, heating for inactivating, cooling to 45-50deg.C, centrifuging, filtering, and collecting filtrate;
4) And sequentially carrying out microfiltration, twice ultrafiltration and nanofiltration on the obtained filtrate to obtain the noni peptide.
Further, the crushing in the step 1) is performed until the particle size is 50-100 meshes.
Further, the operation of adding water in the step 1) is to add water with the mass of 20 times of that of the dried noni fruits, and the temperature of the water is 25-30 ℃;
the addition amount of the sodium bisulfite is 3 per mill of the quality of noni dried fruits;
the addition amount of the ascorbic acid is 1 per mill of the quality of noni dried fruits.
Further, the temperature of the stirring microwave enzymolysis in the step 3) is 50-55 ℃;
the temperature of continuous stirring microwave enzymolysis is 55-60 ℃;
the temperature of heating and inactivating is 85-90 ℃.
Further, the centrifugal filtration in the step 3) is operated by using a three-leg centrifuge and centrifugally filtering with 400-mesh filter cloth.
Further, the microfiltration in step 4) is performed with a 30000 membrane having an interceptable molecular weight of 30000 Da;
the operation of the twice ultrafiltration is as follows: 10000 membranes are adopted for the first ultrafiltration, and 5000 membranes are adopted for the second ultrafiltration;
the nanofiltration is performed by using a 300Da membrane.
The invention also claims noni peptide powder which is obtained by freeze drying noni peptide extracted by the extraction method.
The extraction method has simple and convenient process, easy control, low cost and easy realization of industrial production. The microwave radiation is adopted for assisting enzymolysis, so that energy can be provided for an enzymolysis system, the temperature required by enzymolysis is maintained, the enzymolysis efficiency is greatly improved, the enzymolysis time is reduced, the thoroughness and the accuracy of enzymolysis are ensured, and a new way is provided for the industrial production of noni active ingredients; no harmful substances are generated, and the standard of clean production is reached; the structure of the small molecular peptide is not damaged, the activity of the peptide is maintained, the extraction rate of noni peptide is improved, and the edible value and physiological function of noni are improved; the method has the advantages of simple and convenient production process, high extraction rate and obvious economic benefit.
Detailed Description
The following description of the technical solutions in the embodiments of the present invention will be clear and complete, and it is obvious that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
The method for extracting noni peptides by a microwave-assisted enzymolysis method comprises the following steps:
1) Pulverizing dried noni fruit raw materials to 60 meshes, adding water with the mass of 20 times of that of the dried noni fruit at 25-30 ℃, adding sodium bisulphite with the mass of 3 per mill of that of the dried noni fruit, stirring and soaking for 15min with ascorbic acid with the mass of 1 per mill of that of the dried noni fruit;
2) Grinding the soaked raw materials in a colloid mill, and adjusting the pH value of the slurry to 8.5 at 30 ℃;
3) Placing the slurry in a microwave tank with power of 2-10kw, adjusting microwave power to 2kw, adding alkaline enzyme, stirring at 55deg.C for microwave enzymolysis for 45min, adding papain, continuing stirring at 55deg.C for microwave enzymolysis for 45min, heating to 85deg.C for inactivating, cooling to 45deg.C, centrifuging with 400 mesh filter cloth tripodia centrifuge, and collecting filtrate;
4) Sequentially performing microfiltration, 10000 membrane ultrafiltration, 5000 membrane ultrafiltration and 300Da membrane nanofiltration on the obtained filtrate by using a 30000 membrane with an interceptable molecular weight of 30000Da, and concentrating by using an RO membrane.
The noni peptide extracted by the method is prepared into Cheng Nuoli fruit peptide powder by freeze drying, and the amino acid and protein contents in the powder are detected, and the detection items, the results and the methods are shown in the following table 1:
TABLE 1 detection results of amino acid and protein content in noni peptide powder
Example 2
The method for extracting noni peptides by a microwave-assisted enzymolysis method comprises the following steps:
1) Pulverizing noni dry raw materials to 50 meshes, adding water with the mass of 20 times of that of noni dry at 25 ℃, adding sodium bisulphite with the mass of 3 per mill of that of noni dry, and stirring and soaking for 10min with ascorbic acid with the mass of 1 per mill of that of noni dry;
2) Grinding the soaked raw materials in a colloid mill, and adjusting the pH value of the slurry to 8.5 at 10-50 ℃;
3) Placing the slurry in a microwave tank with power of 2-10kw, adjusting microwave power to 2kw, adding alkaline enzyme, stirring at 50deg.C for microwave enzymolysis for 45min, adding papain, continuing stirring at 55deg.C for microwave enzymolysis for 45min, heating to 85deg.C for inactivating, cooling to 45deg.C, centrifuging with 400 mesh filter cloth tripodia centrifuge, and collecting filtrate;
4) And sequentially carrying out microfiltration, 10000 membrane ultrafiltration, 5000 membrane ultrafiltration and 300Da membrane nanofiltration on the obtained filtrate by using a 30000 membrane with an interceptable molecular weight of 30000 Da.
Example 3
The method for extracting noni peptides by a microwave-assisted enzymolysis method comprises the following steps:
1) Pulverizing noni dry raw materials to 100 meshes, adding water with the mass of 20 times of that of noni dry at 30 ℃, adding sodium bisulphite with the mass of 3 per mill of that of noni dry, stirring and soaking for 15min with ascorbic acid with the mass of 1 per mill of that of noni dry;
2) Grinding the soaked raw materials in a colloid mill, and adjusting the pH value of the slurry to 8.5 at 50 ℃;
3) Placing the slurry in a microwave tank with power of 2-10kw, adjusting microwave power to 2kw, adding alkaline enzyme, stirring at 55deg.C for microwave enzymolysis for 50min, adding papain, stirring at 60deg.C for microwave enzymolysis for 50min, heating to 90deg.C for inactivating, cooling to 50deg.C, centrifuging with 400 mesh filter cloth tripodia centrifuge, and collecting filtrate;
4) Sequentially performing microfiltration, 10000 membrane ultrafiltration, 5000 membrane ultrafiltration and 300Da membrane nanofiltration on the obtained filtrate by using a 30000 membrane with an interceptable molecular weight of 30000Da, and concentrating by using an RO membrane.
Claims (9)
1. A method for extracting noni peptides by a microwave-assisted enzymolysis method, which is characterized by comprising the following steps:
1) Pulverizing dried noni fruit, adding water, adding sodium bisulphite and ascorbic acid, stirring and soaking for 10-15min;
2) Grinding the soaked raw materials in a colloid mill, and adjusting the pH value of the slurry to 8.5 at 10-50 ℃;
3) Placing the slurry in a microwave tank with power of 2-10kw, adding alkaline enzyme, stirring for microwave enzymolysis for 45-50min, adding papain, stirring for microwave enzymolysis for 45-50min, heating for inactivating, cooling to 45-50deg.C, centrifuging, filtering, and collecting filtrate;
4) Sequentially carrying out microfiltration, twice ultrafiltration and nanofiltration on the obtained filtrate to obtain the noni peptide;
the temperature of the stirring microwave enzymolysis in the step 3) is 50-55 ℃;
the temperature of continuous stirring microwave enzymolysis is 55-60 ℃;
the temperature of heating and inactivating is 85-90 ℃.
2. The method for extracting noni peptide by microwave-assisted enzymolysis as claimed in claim 1, wherein the pulverization in step 1) is to a particle size of 50-100 mesh.
3. The method for extracting noni peptide by microwave-assisted enzymolysis according to claim 1, wherein the water adding operation in step 1) is adding water with a mass 20 times that of noni dry fruits, and the temperature of the water is 25-30 ℃;
the addition amount of the sodium bisulfite is 3 per mill of the quality of noni dried fruits;
the addition amount of the ascorbic acid is 1 per mill of the quality of noni dried fruits.
4. The method for extracting noni peptide as claimed in claim 1, wherein the centrifugal filtration in the step 3) is operated by using a three-foot centrifuge and centrifugally filtering with 400 mesh filter cloth.
5. The method for extracting noni peptides by microwave-assisted enzymolysis according to claim 1, wherein the microfiltration in step 4) is performed with 30000 membranes having an interceptable molecular weight of 30000 Da.
6. The method for extracting noni peptide by microwave-assisted enzymolysis as claimed in claim 1, wherein the twice ultrafiltration in step 4) is performed by: 10000 membranes are adopted for the first ultrafiltration, and 5000 membranes are adopted for the second ultrafiltration.
7. The method for extracting noni peptide by microwave-assisted enzymolysis as claimed in claim 1, wherein the nanofiltration in step 4) is performed by using a 300Da membrane.
8. The method for extracting noni peptide by microwave-assisted enzymolysis according to any one of claims 1 to 7, wherein step 4) further comprises an operation of concentrating the treated noni peptide with RO membrane after nanofiltration.
9. Noni peptide powder, characterized in that it is obtained by freeze-drying noni peptide extracted by the method for extracting noni peptide according to any one of claims 1-8 by microwave-assisted enzymolysis.
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