CN109485693A - A method of it extracting quinoa native peptides from quinoa and digests quinoa peptide processed - Google Patents

A method of it extracting quinoa native peptides from quinoa and digests quinoa peptide processed Download PDF

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CN109485693A
CN109485693A CN201811556110.0A CN201811556110A CN109485693A CN 109485693 A CN109485693 A CN 109485693A CN 201811556110 A CN201811556110 A CN 201811556110A CN 109485693 A CN109485693 A CN 109485693A
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quinoa
microwave
suspension slurry
peptide
temperature
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闫建国
刘艳飞
徐贺楠
叶峥
段美青
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Shanxi Sheng Yuan Health Care Products Co Ltd
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    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/145Extraction; Separation; Purification by extraction or solubilisation
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/34Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/06Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00Technologies relating to chemical industry
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    • Y02P20/59Biological synthesis; Biological purification

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Abstract

The method that the invention discloses a kind of to extract quinoa native peptides from quinoa and digest quinoa peptide processed, this method simple process is easily controllable, is easy to realize industrial production, and provides new way for quinoa food industrialization;Unharmful substance generates in production process, has reached the standard of clean manufacturing;Meanwhile improving the edible value and physiological function of quinoa.

Description

A method of it extracting quinoa native peptides from quinoa and digests quinoa peptide processed
Technical field
The present invention relates to plant processing technique field, specially one kind extracts quinoa native peptides and enzymatic hydrolysis Chenopodiaceae from quinoa The method of wheat peptide.
Background technique
Quinoa Shu Li section dicotyledon, plant height meter differ, plant is in because of growth conditions difference from tens centimetres to three Broom shape, root system shallow root, sequence locusta, self-pollination, leading shoot and the side tip can set seeds, and seed is round sheet, 1.5 ㎜ of diameter To 2 ㎜.Appearance color has white, milk yellow, aubergine and black.Wherein black and the seed of aubergine are smaller, white and cream Yellow taste good.
The plant of quinoa, not universal all over the world, source area is andes region, be mainly distributed on Bolivia, The states such as Peru, Ecuador.China introduces at first in Tibet Autonomous Region in last century end and plants experimentally, and plants experimentally into Shanxi Province within 2008 After function, as scale of the project plant of shaking off poverty, later in provinces and regions such as Qinghai, Gansu, Inner Mongol, Yunnan, also successive introduction of plant. According to statistics, by year ends 2011, nearly 150,000 mu of China's planting area, be now in the trend of rapid expansion.
Though quinoa is plant, the complete protein that there is animal just to have for it, this is very rare.Its endosperm accounts for The 68% of plantation, and there are nutritional activities, protein content is up to 16%-22%, and quality is suitable with milk powder and meat;Rich in more Kind amino acid, wherein having necessary to human body, all 9 kinds of essential amino acids, appropriate its of ratio are easy to absorb, be especially enriched in plant The lysine being rich in, the mineral matter nutritionals such as calcium, magnesium, phosphorus, potassium, iron, zinc, selenium, manganese, copper content is high, is rich in unsaturated fatty acid, class The multiple beneficials compound such as flavones, B family vitamin and vitamin E, choline, glycine betaine, folic acid, alpha-linolenic acid, beta glucan, meals Edible cellulose content is up to 7.1%, and cholesterol 0 is free of seitan, and low fat is (305kcal/100g) low in calories, low glycemic (GI Glycemic value 35, low glycemic standard be 55), be nearly all classic in common food.
Studies have reported that claiming: quinoa is easy ripe digestible basic food, unique in taste, there is a strand fragrant, can be balanced It supplements the nutrients, enhances body function, repair constitution, adjust endocrine, all there is auxiliary treatment function to weight-reducing, prevention disease and anticancer Can, it is suitble to all groups edible, is particularly suitable for hyperglycemia, hypertension, hyperlipidemia, heart disease chronic diseases patient and baby children The special constitution such as youngster, pregnant woman, children, old man and the irregular crowd that lives, are eaten for a long time significant effect.
Occupy the nutritional characteristic and physiological function of above-mentioned quinoa, FAO (Food and Agriculture Organization of the United Nation) (WHO), it is believed that quinoa is a kind of list Body plant can substantially meet the food of human body basic nutrition, and recommending quinoa is the wholefood of the optimum mankind, therefore, connection It closes state and was set to quinoa year for 2013, it is therefore intended that promote human nutrition health and food safety.
But up to the present, we are essentially identical to the eating method and five cereals of quinoa, and this eating method is to Chenopodiaceae The peculiar native peptides of wheat cause the quinoa food of different degrees of destruction, especially extruding, natural peptide activity therein almost quilt entirely It destroys.Therefore, deep processing is carried out to quinoa and retains its original edible value and physiological function, and is further improved with must The property wanted.
Summary of the invention
The method that the purpose of the present invention is to provide a kind of to extract quinoa native peptides from quinoa and digest quinoa peptide processed is used In solution above-mentioned technical problem.
For improve quinoa edible value and physiological function, The technical solution adopted by the invention is as follows:
From quinoa extract quinoa native peptides method the following steps are included:
S001: the impurity in quinoa kernel is removed, places it under sunlight or removes in drying chamber extra moisture, make Chenopodiaceae The moisture content of wheat seeds benevolence is lower than 13%, and storage is stand-by;
S002: being impregnated quinoa kernel 4-5 hours with 50-60 DEG C of deionized water, is allowed to the heart expansion that sufficiently absorbs water, wherein Quinoa kernel and the solid-to-liquid ratio of deionized water are 1:4;
S003: the quinoa kernel impregnated being poured into together together with deionized water and carries out defibrination in fiberizer, is formed and is suspended Slurries, further, the fineness of the defibrination is can sieve No. 2 sieves by national standard all as standard;
S004: being added deionized water in the suspension slurry of milled, its solid-to-liquid ratio is made to reach 1:8;
S005: being added the NaOH solution that concentration is 20% in suspension slurry, adjusts the pH value of suspension slurry to 7.5;
S006: the suspension slurry that pH value is 7.5 is placed in microwave abstracting tank, starts the minimum power shelves of microwave abstracting tank Position is heated up, and 60 DEG C of temperature control, microwave total time is 30min, keeps the temperature 30min in the environment of 50-60 DEG C again later, further Ground, the frequency of the microwave abstracting tank are 2450MHz;
S007: the suspension slurry that microwave treatment is crossed is discharged, and is cooled to temperature less than 45 DEG C, later with 200 mesh filter clothes three Sufficient centrifuge filtering, wherein residue collection does subsequent enzymatic hydrolysis raw material, and permeate carries out next step operation;
S008: through 200 mesh filter cloth tripod pendulum type batch centrifugal filtered permeate micro-filtration membrane microfiltrations and with 10000Da and 5000Da ultrafiltration membrane ultrafiltration, the dense night of micro-filtration and the dense night of two-stage ultrafiltration are merged into after the filtering of 200 mesh filter cloth tripod pendulum type batch centrifugals Filter residue in do subsequent enzymatic hydrolysis raw material;
S009: the permeate after 5000Da ultrafiltration membrane ultrafiltration is put into rotary evaporator and is concentrated, 60 DEG C of temperature control, dense 20 ° of B é are reduced to, concentrate is discharged and is cooled to room temperature, then are placed in energy cryogenic refrigeration into -30 DEG C of freezer or refrigerator-freezer, freezing To -30 DEG C, and kept for 1 hour;
S010: taking out and be refrigerated to -30 DEG C of ice cube, is smashed to less than the fritter of 40mm, and micro-wave vacuum is placed in Dry in machine, 60 DEG C of temperature control, drying to its moisture content terminates drying process when being lower than 7%;
S011: obtaining the natural peptide product of quinoa, and product colour is mainly distributed as 4 peptides extremely close to quinoa kernel color, peptide fragment 12 peptides, 85% or more of the total peptide amount of Zhan.
The method for digesting quinoa peptide processed, comprising the following steps:
S101: will be filtered through 200 mesh filter cloth tripod pendulum type batch centrifugals in quinoa native peptides step S008 from being extracted in quinoa Simultaneously, appropriate deionized water is added in the dense silk tree of filter residue, the dense night of micro-filtration and two-stage ultrafiltration, and being configured to concentration is the outstanding of 7%-8% Laitance liquid;
S102: the NaOH solution that concentration is 20% is added in the suspension slurry of S101 configuration, adjusts its pH value to 9.0;
S103: the suspension slurry that pH value is 9.0 is placed in microwave abstracting tank, starts the minimum power shelves of microwave abstracting tank Position is heated up, and 58 DEG C of temperature control, microwave total time is 30min, keeps the temperature 30min in the environment of 50-58 DEG C again later, further Ground, the frequency of the microwave abstracting tank are 2450MHz;
S104: the suspension slurry that microwave treatment is crossed is discharged, and is cooled to temperature less than 45 DEG C, molten with 15% acetic acid later The chitosan solution of solution 1.5% adjusts the pH value of suspension slurry to 5.0, makes protein, starch, carbohydrate, lipid of non-alkali soluble etc. It settles out, stands 1-2 small layereds;
S105: the suspension slurry that precipitating is layered is filtered with 400 mesh filter cloth tripod pendulum type batch centrifugals, residue collection waits for With permeate carries out next step operation;
S106: PH is adjusted through the NaOH solution that the 400 filtered permeate concentration of mesh filter cloth tripod pendulum type batch centrifugal are 20% Value adds alkali protease, additive amount is the 2.5 ‰ of amount of liquid, and further, the enzyme activity of the alkali protease is to 8.5 200000 u/g;
S107: being placed in microwave abstracting tank, microwave-assisted enzymatic hydrolysis, and 60 DEG C of temperature control, microwave total time is 30min, then in 50- 30min is kept the temperature in the environment of 60 DEG C, detects the pH value of enzymolysis liquid, when pH value is less than 7.5, the Na0H solution for being 20% with concentration PH value is adjusted to 7.5, adds papain afterwards, additive amount is digest liquid measure 1.5 ‰, when pH value is greater than 7.5, is directly added Adding papain, additive amount is digest liquid measure 1.5 ‰, continue microwave-assisted enzymatic hydrolysis later, 55 DEG C of temperature control, microwave total time For 20min, then 20min is kept the temperature in the environment of 45-55 DEG C, further, the enzyme activity of the papain is 500,000 u/g;
S108: after the enzymatic hydrolysis of papain, enzymolysis liquid being placed in microwave abstracting tank, starts maximum power shelves Position is warming up to 85-90 DEG C, keeps the temperature 5-8min, and enzyme deactivation is living;
S109: the enzymolysis liquid living of enzyme deactivation is discharged, rotary evaporator is put into and is concentrated, is concentrated into 20 ° of B é, is terminated dense Contracting process;
S110: concentrate is discharged, and is cooled to room temperature, then is placed in energy cryogenic refrigeration into -35 DEG C of freezer or refrigerator-freezer, cold Freeze to -35 DEG C, and is kept for 1 hour;
S111: taking out and be refrigerated to -35 DEG C of ice cube, is smashed to less than the fritter of 40mm, and micro-wave vacuum is placed in Dry in machine, drying to its moisture content terminates drying process when being lower than 7%;
S112: obtaining the enzymatic hydrolysis quinoa peptide of the micro- ash of white, and peptide fragment is mainly distributed as 4 peptides to 12 peptides, and the 75% of the total peptide amount of Zhan More than;
S113: filter residue obtained by the suspension slurry of the layering of precipitating through the filtering of 400 mesh filter cloth tripod pendulum type batch centrifugals is small in temperature It is dried, is obtained using starch as main component under conditions of 75 DEG C, protein content is no less than 6%, while containing a small amount of sugar The quinoa powder of class, lipid.
Compared with prior art, the beneficial effects of the present invention are:
The present invention extracts quinoa native peptides from quinoa and digests the simple process of quinoa peptide processed, easily controllable, Yi Shixian Industrialized production provides new way for quinoa food industrialization;Unharmful substance generates in production process, has reached cleaning life The standard of production;Meanwhile improving the edible value and physiological function of quinoa.
Specific embodiment
Following specific embodiments are not intended to limit the scope of the invention for illustrating the present invention.If not it illustrates, Raw material and equipment used is commercially available.
From quinoa extract quinoa native peptides method the following steps are included:
S001: the impurity in quinoa kernel is removed, places it under sunlight or removes in drying chamber extra moisture, make Chenopodiaceae The moisture content of wheat seeds benevolence is lower than 13%, and storage is stand-by;
S002: being impregnated quinoa kernel 4-5 hours with 50-60 DEG C of deionized water, is allowed to the heart expansion that sufficiently absorbs water, wherein Quinoa kernel and the solid-to-liquid ratio of deionized water are 1:4;
S003: the quinoa kernel impregnated being poured into together together with deionized water and carries out defibrination in fiberizer, is formed and is suspended Slurries, it is preferred that the fineness of the defibrination is can sieve No. 2 sieves by national standard all as standard;
S004: being added deionized water in the suspension slurry of milled, its solid-to-liquid ratio is made to reach 1:8;
S005: being added the NaOH solution that concentration is 20% in suspension slurry, adjusts the pH value of suspension slurry to 7.5;
S006: the suspension slurry that pH value is 7.5 is placed in microwave abstracting tank, starts the minimum power shelves of microwave abstracting tank Position is heated up, and 60 DEG C of temperature control, microwave total time is 30min, keeps the temperature 30min in the environment of 50-60 DEG C again later, preferably , the frequency of the microwave abstracting tank is 2450MHz;
S007: the suspension slurry that microwave treatment is crossed is discharged, and is cooled to temperature less than 45 DEG C, later with 200 mesh filter clothes three Sufficient centrifuge filtering, wherein residue collection does subsequent enzymatic hydrolysis raw material, and permeate carries out next step operation;
S008: through 200 mesh filter cloth tripod pendulum type batch centrifugal filtered permeate micro-filtration membrane microfiltrations and with 10000Da and 5000Da ultrafiltration membrane ultrafiltration, the dense night of micro-filtration and the dense night of two-stage ultrafiltration are merged into after the filtering of 200 mesh filter cloth tripod pendulum type batch centrifugals Filter residue in do subsequent enzymatic hydrolysis raw material;
S009: the permeate after 5000Da ultrafiltration membrane ultrafiltration is put into rotary evaporator and is concentrated, 60 DEG C of temperature control, dense 20 ° of B é are reduced to, concentrate is discharged and is cooled to room temperature, then are placed in energy cryogenic refrigeration into -30 DEG C of freezer or refrigerator-freezer, freezing To -30 DEG C, and kept for 1 hour;
S010: taking out and be refrigerated to -30 DEG C of ice cube, is smashed to less than the fritter of 40mm, and micro-wave vacuum is placed in Dry in machine, 60 DEG C of temperature control, drying to its moisture content terminates drying process when being lower than 7%;
S011: obtaining the natural peptide product of quinoa, and product colour is mainly distributed as 4 peptides extremely close to quinoa kernel color, peptide fragment 12 peptides, 85% or more of the total peptide amount of Zhan.
The method for digesting quinoa peptide processed, comprising the following steps:
S101: will be filtered through 200 mesh filter cloth tripod pendulum type batch centrifugals in quinoa native peptides step S008 from being extracted in quinoa Simultaneously, appropriate deionized water is added in the dense silk tree of filter residue, the dense night of micro-filtration and two-stage ultrafiltration, and being configured to concentration is the outstanding of 7%-8% Laitance liquid;
S102: the NaOH solution that concentration is 20% is added in the suspension slurry of S101 configuration, adjusts its pH value to 9.0;
S103: the suspension slurry that pH value is 9.0 is placed in microwave abstracting tank, starts the minimum power shelves of microwave abstracting tank Position is heated up, and 58 DEG C of temperature control, microwave total time is 30min, keeps the temperature 30min in the environment of 50-58 DEG C again later, preferably , the frequency of the microwave abstracting tank is 2450MHz;
S104: the suspension slurry that microwave treatment is crossed is discharged, and is cooled to temperature less than 45 DEG C, molten with 15% acetic acid later The chitosan solution of solution 1.5% adjusts the pH value of suspension slurry to 5.0, makes protein, starch, carbohydrate, lipid of non-alkali soluble etc. It settles out, stands 1-2 small layereds;
S105: the suspension slurry that precipitating is layered is filtered with 400 mesh filter cloth tripod pendulum type batch centrifugals, residue collection waits for With permeate carries out next step operation;
S106: PH is adjusted through the NaOH solution that the 400 filtered permeate concentration of mesh filter cloth tripod pendulum type batch centrifugal are 20% Value adds alkali protease, additive amount is the 2.5 ‰ of amount of liquid, it is preferred that the enzyme activity of the alkali protease is 20 to 8.5 Ten thousand u/g;
S107: being placed in microwave abstracting tank, microwave-assisted enzymatic hydrolysis, and 60 DEG C of temperature control, microwave total time is 30min, then in 50- 30min is kept the temperature in the environment of 60 DEG C, detects the pH value of enzymolysis liquid, when pH value is less than 7.5, the Na0H solution for being 20% with concentration PH value is adjusted to 7.5, adds papain afterwards, additive amount is digest liquid measure 1.5 ‰, when pH value is greater than 7.5, is directly added Adding papain, additive amount is digest liquid measure 1.5 ‰, continue microwave-assisted enzymatic hydrolysis later, 55 DEG C of temperature control, microwave total time For 20min, then in the environment of 45-55 DEG C keep the temperature 20min, it is preferred that the enzyme activity of the papain is 500,000 u/g;
S108: after the enzymatic hydrolysis of papain, enzymolysis liquid being placed in microwave abstracting tank, starts maximum power shelves Position is warming up to 85-90 DEG C, keeps the temperature 5-8min, and enzyme deactivation is living;
S109: the enzymolysis liquid living of enzyme deactivation is discharged, rotary evaporator is put into and is concentrated, is concentrated into 20 ° of B é, is terminated dense Contracting process;
S110: concentrate is discharged, and is cooled to room temperature, then is placed in energy cryogenic refrigeration into -35 DEG C of freezer or refrigerator-freezer, cold Freeze to -35 DEG C, and is kept for 1 hour;
S111: taking out and be refrigerated to -35 DEG C of ice cube, is smashed to less than the fritter of 40mm, and micro-wave vacuum is placed in Dry in machine, drying to its moisture content terminates drying process when being lower than 7%;
S112: obtaining the enzymatic hydrolysis quinoa peptide of the micro- ash of white, and peptide fragment is mainly distributed as 4 peptides to 12 peptides, and the 75% of the total peptide amount of Zhan More than;
S113: filter residue obtained by the suspension slurry of the layering of precipitating through the filtering of 400 mesh filter cloth tripod pendulum type batch centrifugals is small in temperature It is dried, is obtained using starch as main component under conditions of 75 DEG C, protein content is no less than 6%, while containing a small amount of sugar The quinoa powder of class, lipid.
Compared with prior art, the beneficial effects of the present invention are:
The present invention extracts quinoa native peptides from quinoa and digests the simple process of quinoa peptide processed, easily controllable, Yi Shixian Industrialized production provides new way for quinoa food industrialization;Unharmful substance generates in production process, has reached cleaning life The standard of production;Meanwhile improving the edible value and physiological function of quinoa.

Claims (5)

1. a kind of method extracted quinoa native peptides from quinoa and digest quinoa peptide processed, it is characterised in that: extracted from quinoa The methods of quinoa native peptides the following steps are included:
S001: the impurity in quinoa kernel is removed, places it under sunlight or removes in drying chamber extra moisture, make quinoa kind The moisture content of benevolence is lower than 13%, and storage is stand-by;
S002: being impregnated quinoa kernel 4-5 hours with 50-60 DEG C of deionized water, is allowed to the heart expansion that sufficiently absorbs water, wherein quinoa Kernel and the solid-to-liquid ratio of deionized water are 1:4;
S003: the quinoa kernel impregnated being poured into together together with deionized water and carries out defibrination in fiberizer, forms suspension slurry;
S004: being added deionized water in the suspension slurry of milled, its solid-to-liquid ratio is made to reach 1:8;
S005: being added the NaOH solution that concentration is 20% in suspension slurry, adjusts the pH value of suspension slurry to 7.5;
S006: by pH value be 7.5 suspension slurry be placed in microwave abstracting tank, start microwave abstracting tank minimum power gear into Row heating, 60 DEG C of temperature control, microwave total time is 30min, keeps the temperature 30min in the environment of 50-60 DEG C again later;
S007: the suspension slurry that microwave treatment is crossed is discharged, and is cooled to temperature less than 45 DEG C, later with 200 mesh filter cloth tripodias from Scheming filtering, wherein residue collection does subsequent enzymatic hydrolysis raw material, and permeate carries out next step operation;
S008: surpass through 200 mesh filter cloth tripod pendulum type batch centrifugal filtered permeate micro-filtration membrane microfiltrations and with 10000Da and 5000Da Ultrafiltration through membranes, the dense night of micro-filtration and the dense night of two-stage ultrafiltration are merged into through in the 200 filtered filter residues of mesh filter cloth tripod pendulum type batch centrifugal Do subsequent enzymatic hydrolysis raw material;
S009: the permeate after 5000Da ultrafiltration membrane ultrafiltration is put into rotary evaporator and is concentrated, and 60 DEG C of temperature control, is concentrated into 20 ° of B é, concentrate is discharged and is cooled to room temperature, then is placed in energy cryogenic refrigeration into -30 DEG C of freezer or refrigerator-freezer, be refrigerated to - 30 DEG C, and kept for 1 hour;
S010: taking out and be refrigerated to -30 DEG C of ice cube, is smashed to less than the fritter of 40mm, is placed in microwave vacuum dryer Dry, 60 DEG C of temperature control, drying to its moisture content terminates drying process when being lower than 7%;
S011: obtaining the natural peptide product of quinoa, and for product colour close to quinoa kernel color, peptide fragment is mainly distributed as 4 peptides to 12 peptides, 85% or more of the total peptide amount of Zhan;
The method for digesting quinoa peptide processed, comprising the following steps:
S101: will from quinoa extract quinoa native peptides step S008 in through the filtered filter residue of 200 mesh filter cloth tripod pendulum type batch centrifugals, Simultaneously, appropriate deionized water is added in the dense silk tree of dense night of micro-filtration and two-stage ultrafiltration, is configured to the suspension that concentration is 7%-8% and starches Liquid;
S102: the NaOH solution that concentration is 20% is added in the suspension slurry of S101 configuration, adjusts its pH value to 9.0;
S103: by pH value be 9.0 suspension slurry be placed in microwave abstracting tank, start microwave abstracting tank minimum power gear into Row heating, 58 DEG C of temperature control, microwave total time is 30min, keeps the temperature 30min in the environment of 50-58 DEG C again later;
S104: the suspension slurry that microwave treatment is crossed is discharged, and is cooled to temperature less than 45 DEG C, later with 15% acetate dissolution 1.5% chitosan solution adjusts the pH value of suspension slurry to 5.0, keeps protein, starch, carbohydrate, lipid of non-alkali soluble etc. heavy Out, 1-2 small layereds are stood;
S105: the suspension slurry that precipitating is layered is filtered with 400 mesh filter cloth tripod pendulum type batch centrifugals, residue collection is stand-by, thoroughly It crosses liquid and carries out next step operation;
S106: through the 400 filtered permeate concentration of mesh filter cloth tripod pendulum type batch centrifugal be 20% NaOH solution adjust pH value to 8.5, alkali protease is added, additive amount is the 2.5 ‰ of amount of liquid;
S107: being placed in microwave abstracting tank, microwave-assisted enzymatic hydrolysis, and 60 DEG C of temperature control, microwave total time is 30min, then at 50-60 DEG C In the environment of keep the temperature 30min, detect the pH value of enzymolysis liquid, when pH value is less than 7.5, with concentration be 20% Na0H solution adjust PH value adds papain, additive amount is digest liquid measure 1.5 ‰, and when pH value is greater than 7.5, directly addition is wooden to 7.5 afterwards Melon protease, additive amount are digest liquid measure 1.5 ‰, continue microwave-assisted enzymatic hydrolysis later, and 55 DEG C of temperature control, microwave total time is 20min, then 20min is kept the temperature in the environment of 45-55 DEG C;
S108: after the enzymatic hydrolysis of papain, enzymolysis liquid being placed in microwave abstracting tank, starts maximum power gear, is risen Temperature keeps the temperature 5-8min to 85-90 DEG C, and enzyme deactivation is living;
S109: the enzymolysis liquid living of enzyme deactivation being discharged, rotary evaporator is put into and is concentrated, is concentrated into 20 ° of B é, terminates concentration work Sequence;
S110: concentrate is discharged, and is cooled to room temperature, then is placed in energy cryogenic refrigeration into -35 DEG C of freezer or refrigerator-freezer, freezing To -35 DEG C, and kept for 1 hour;
S111: taking out and be refrigerated to -35 DEG C of ice cube, is smashed to less than the fritter of 40mm, is placed in microwave vacuum dryer Dry, drying to its moisture content terminates drying process when being lower than 7%;
S112: obtaining the enzymatic hydrolysis quinoa peptide of the micro- ash of white, and peptide fragment is mainly distributed as 4 peptides to 12 peptides, and 75% or more of the total peptide amount of Zhan;
S113: filter residue obtained by the suspension slurry of the layering of precipitating through the filtering of 400 mesh filter cloth tripod pendulum type batch centrifugals, in temperature less than 75 It is dried, is obtained using starch as main component under conditions of DEG C, protein content is no less than 6%, while containing a small amount of carbohydrate, rouge The quinoa powder of class.
2. a kind of method extracted quinoa native peptides from quinoa and digest quinoa peptide processed according to claim 1, special Sign is: the fineness of defibrination is in the S003 can sieve No. 2 sieves by national standard all as standard.
3. a kind of method extracted quinoa native peptides from quinoa and digest quinoa peptide processed according to claim 1, special Sign is: the frequency of the microwave abstracting tank is 2450MHz.
4. a kind of method extracted quinoa native peptides from quinoa and digest quinoa peptide processed according to claim 1, special Sign is: the enzyme activity of the alkali protease is 200,000 u/g.
5. a kind of method extracted quinoa native peptides from quinoa and digest quinoa peptide processed according to claim 1, special Sign is: the enzyme activity of the papain is 500,000 u/g.
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CN110438178A (en) * 2019-08-14 2019-11-12 甘肃省轻工研究院有限责任公司 A method of amino acid is extracted using quinoa slag
CN111165646A (en) * 2020-01-13 2020-05-19 山西原生肽科技有限公司 Quinoa active peptide and preparation method and application thereof
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CN112772825A (en) * 2021-01-04 2021-05-11 山西昇力元生物科技有限公司 Method for reducing heavy metal cadmium in ganoderma lucidum spore powder
CN113337563A (en) * 2021-06-01 2021-09-03 科丽思化妆品(上海)有限公司 Quinoa peptide with whitening and antioxidant activities and preparation method and application thereof
CN113337563B (en) * 2021-06-01 2022-09-30 科丽思化妆品(上海)有限公司 Quinoa peptide with whitening and antioxidant activities and preparation method and application thereof

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