CN107319527A - Giant salamander nutrient powder product is prepared using radix polygonati officinalis - Google Patents
Giant salamander nutrient powder product is prepared using radix polygonati officinalis Download PDFInfo
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- CN107319527A CN107319527A CN201710550446.5A CN201710550446A CN107319527A CN 107319527 A CN107319527 A CN 107319527A CN 201710550446 A CN201710550446 A CN 201710550446A CN 107319527 A CN107319527 A CN 107319527A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
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Abstract
The present invention provide prepare giant salamander nutrient powder product using radix polygonati officinalis, take 20~40 parts of polygonati rhizoma, 10~15 parts of saponin rice, 40~60 parts of giant salamander, after high temperature flooding, enzymolysis, allotment, drying for standby.The present invention takes full advantage of protein and polysaccharide and Flavonoid substances in giant salamander, radix polygonati officinalis and saponin rice, and the fishlike smell that giant salamander during traditional handicraft produces is overcome again, with invigorating the spleen moistening lung, beauty treatment, the effect such as enriching yin.This method avoids the loss of giant salamander nutrient protein composition, giant salamander is handled by deep processings such as high-temperature process, enzymolysis, the comprehensive process of giant salamander is realized, process is easy to control, is easy to industrialized production, also increase giant salamander processing added value.Using complex cellulase and protease hydrolytic, be conducive to fragrant solomonseal rhizome polyoses quick release, improve radix polygonati officinalis service efficiency, improve essential amino acids content in product.Protein content in raw material is effectively increased by saponin rice germination treatment, glucose content in saponin rice is reduced again, amino acid content is improved by proteasome degradation, polyphenoils in germination saponin rice can be made full use of again.
Description
Technical field
Giant salamander nutrient powder product and preparation method thereof is prepared using radix polygonati officinalis the present invention relates to one kind, belongs to foods processing technique
Field.
Background technology
Radix polygonati officinalis be Liliaceae Polygonatum herbaceos perennial, also known as solomon's seal, ground tube, tail ginseng, small bell dish, ring Huang,
Bamboo root seven, sweet tea root vegetables etc..Be used as medicine with its dry rhizome, with the name of solomon's seal first recorded in《Sheng Nong's herbal classic》, top grade is classified as, its property
It is flat, it is sweet, be slightly cold, there is yin-nourishing, moisturize, relieving restlessness, quench the thirst, belong to one of Chinese medicine of conventional medicine-food two-purpose.Radix polygonati officinalis root
The glucosides of steroid saponin, especially steroidal spiral saponin(e and five rings sterol is mainly contained in stem, and containing polysaccharide, flavones and its glucosides
And a variety of anthraquinone analog compounds.Recent pharmacological research shows that radix polygonati officinalis has strengthen immunity, expansion blood vessel, resists the acute heart
Myocardial ischemia, decompression, anti-aging, antibacterial, reducing blood lipid, antiatherosclerosis, antitumor etc. are acted on.Radix polygonati officinalis is used to treat heat now
Disease, emphasizes its nourishing Yin and moistening lung, the work(for nourishing the stomach of promoting the production of body fluid.
Saponin rice is also known as Semen Saussureae Involueratae, saponin rice, gleditsia sinensis benevolence, gleditsia sinensis essence, is the fruit of Chinese honey locust.Saponin rice gross energy is 3.36
Kilocalorie/gram, total carbohydrates 61%, total amino acid content is 3.13%, contains 7 kinds of vitamins, rich in nutrition content, nutrient
Species is more, category high-energy, Hi CHO, low albumen, low fat food, and contains vegetable diet fiber..With foster
Heart and inducing arteries and veins, clear liver and improve vision, nourishing spleen and kidney, eliminating the phlegm are had one's ideas straightened out, dredge intestines diuresis, moisturizing beauty treatment, refresh oneself tonifying Qi the effects such as.Add with discharging water
Thermal expansion, colloid is translucent, fragrant glutinous profit mouth, is the precious fully natural green nourishing food of mediation human-body viscera function.
Giant salamander (Andrias davidianus) is the maximum amphibian of existing individual, belongs to national two classes protection animal.
It is widely distributed in China, in the mountain stream river for mainly originating in the Changjiang river, the Yellow River and Zhujiang River middle and upper reaches tributary, especially with Sichuan, Hubei, lake
It is many that south, Guizhou, Shaanxi etc., which are saved,.Giant salamander nutrient value is high, contains good protein and abundant amino acid, trace element.Its
Contain more than 50 kinds of natural bioactive thing in body.These biological active matters are in the side such as edible, medicine, cosmetics, healthy food
Face has unique research and development value.There is document report to prepare giant salamander oligosaccharide peptide from giant salamander skin mucus, it is anti-by mouse
Fatigue experiment, it is found that it has anti-fatigue effect.Giant salamander oligosaccharide peptide also has good hygroscopicity and stronger uvioresistant
Activity.
In giant salamander oligosaccharide peptide in 18 kinds of contained amino acid in addition to tryptophan is not measured, the required amino of other 7 kinds of human body
(baby institute is required for sour (threonine, valine, methionine, phenylalanine, isoleucine, leucine, lysine) and histidine
Amino acid) content it is higher.Threonine content enriches the most wherein in essential amino acid, and up to 13.1%.Next to that day
Winter propylhomoserin/acid amides (Asx) and glutamic acid/acid amides (Glx) are respectively 11.2% and 12.5%.And serine content is minimum
0.2%.
Publication No. CN101812116A China discloses a kind of giant salamander oligosaccharide peptide, preparation method, as follows
Carry out：A. giant salamander mucus is homogenized at 0~10 DEG C；B. into homogenate add 1~3 times of volume pH 7~8 0.01~
0.1M phosphate buffers and the complex enzyme for being 0.1~1% with homogenate mass ratio, digest 12~48h；The complex enzyme is by ocean
Alkali protease, papain, pepsin and trypsase are mixed in unit of activity ratio for 3: 3: 2: 2 ratios；C. centrifuge
Supernatant is taken, uses molecular cut off to be separated for below 4000Da Ultra filtration membrane device, takes the liquid through milipore filter；D. pass through
Sephadex LH-20 molecular sieve chromatographies, collect OD280nm absworption peaks；E. collected liquid is passed through into macroporous absorbent resin
And be freeze-dried after activated carbon, giant salamander oligosaccharide peptide is made.
Publication No. CN101991038A China discloses a kind of giant salamander oligosaccharide peptide health food, by mass percentage
Counting effective ingredient is：Giant salamander oligosaccharide peptide 10~80%, oyster polysaccharide 1~50%, mussel polysaccharide 1~50%, vitamin C 1~
50% and taurine 1~50%, wherein giant salamander mucus is homogenized at 0~10 DEG C, and 1~3 times of volume pH is added into homogenate
7~8 0.01~0.1M phosphate buffers and the complex enzyme for being 0.1~1% with homogenate mass ratio, digest 12~48h, centrifuging and taking
Supernatant, uses molecular cut off to be separated for below 4000Da Ultra filtration membrane device, takes the liquid through milipore filter, pass through
Sephadex LH-20 molecular sieve chromatographies, collect OD280nm absworption peaks, by collected liquid by macroporous absorbent resin and
It is freeze-dried after activated carbon, giant salamander oligosaccharide peptide is made.
Publication No. CN102676622A China discloses a kind of Andrias davidianus nano peptide and andrias davidianus wine, makes as follows
It is standby：A. the giant salamander after slaughtering is cleaned, takes Megalobatrachus japonicus daoidianuas(Blanchard) to be freeze-dried to moisture and be less than 1%, by lyophilized Megalobatrachus japonicus daoidianuas(Blanchard) ultramicro grinding
Into the Ultramicro-powder of 500~5000 mesh；B. by giant salamander Ultramicro-powder ball milling into 50~200nm giant salamander nano powder；C. to giant salamander nanometer
0.01~0.1M the phosphate buffers and complex enzyme of pH7~8 are added in powder, 12~48h, the giant salamander nano powder and 0.01 is digested
The mass ratio of~0.1M phosphate buffers is 1：1~10, the complex enzyme addition is 10~25 unit of activity/giant salamander nano powder
(g) it by ocean acid protease, papain, pepsin by unit of activity ratio is 3 that, the complex enzyme, which is,：3：4 ratios are mixed
Close；D. centrifuging and taking supernatant, uses molecular cut off to be separated for below 4000Da Ultra filtration membrane device, takes the liquid through milipore filter
Body, by collected liquid by being freeze-dried after macroporous absorbent resin and activated carbon, is made nano peptide and andrias davidianus.
Publication No. CN103609832A China discloses a kind of giant salamander active protein powder and preparation method thereof, by including
The method of following steps is made：(1) meat gruel is made in giant salamander raw material, adds the water of 3.5~4.5 times of giant salamander raw material weight, stirring
Uniformly, the giant salamander raw material is Megalobatrachus japonicus daoidianuas(Blanchard) and giant salamander mixed material, and the giant salamander mixed material is giant salamander skin, giant salamander liver, giant salamander
Secretion, giant salamander blood, giant salamander stomach, the one or more of Giant salamander bone, the weight proportion of Megalobatrachus japonicus daoidianuas(Blanchard) and giant salamander mixed material for 90~
98%：2~10%；(2) compound protease of giant salamander raw material weight 0.3~0.5% is added, in temperature 45 C~55 DEG C, pH6~7
Lower enzyme digestion reaction 3-5h, hydrolyzate boils the enzyme 5-10min that goes out, and filtering can obtain giant salamander active proteolytic liquid, described compound
Protease is alkali protease, papain, the mixture of flavor protease；(3) by salamander active proteolytic liquid in 80-
100 DEG C of enzymes that go out, filterings, are concentrated and dried, obtain giant salamander albumen powder；In step (1), Megalobatrachus japonicus daoidianuas(Blanchard) and giant salamander skin in the giant salamander raw material,
Giant salamander liver, giant salamander secretion, giant salamander blood, giant salamander stomach, the weight proportion of Giant salamander bone are 100：0.5~2:0.5~1：0.1~0.5:
0.5~1:0.5~1:0.5~2.
Publication No. CN104206641A China discloses a kind of giant salamander polypeptide powder and preparation method thereof, and being will be big
Salamander meat gruel and water are mixed with the ratio of solid-to-liquid ratio 1: 2~1: 5, add potassium citrate, the meat gruel of meat gruel quality 0.1%~0.55%
The neutral proteinase or flavor protease of quality 0.1%~1% are digested, and enzymolysis pH is 6.5~7.5, and temperature is 40~60
DEG C, 1~3h of enzymolysis time；The compound protease of giant salamander meat gruel quality 0.1%~5% is added, temperature is maintained at 40~60 DEG C,
Continue to digest 2~3h.Product heats maintain 10~15min to 100 DEG C after enzymolysis, filter to obtain polypeptide hydrolyzate, add big
The activated carbon of salamander meat gruel quality 0.1%~3%, filtering obtains polypeptide hydrolyzate, carries out reduced vacuum concentration, vacuum refrigeration
Dry, obtain giant salamander polypeptide powder.
Publication No. CN104432083A China discloses a kind of preparation method of giant salamander polypeptide powder Antioxidation Oral Liquid, its
It is characterised by, methods described comprises the following steps：1) it is raw material to choose artificial feeding Megalobatrachus japonicus daoidianuas(Blanchard), rejects grease and impurity, is cleaned
After draining, add quality and carry out homogenized for the pure water of 2 times of Megalobatrachus japonicus daoidianuas(Blanchard)；2) in mass ratio 2:1 ratio, by pure water plus
Enter step 1) Megalobatrachus japonicus daoidianuas(Blanchard) after homogenized, add complex enzyme and digested, compound enzyme dosage is to drain giant salamander meat quality
0.5%-2%, hydrolysis temperature is 50-60 DEG C, and enzymolysis time is 5-7h, and enzymolysis finishes heating enzymolysis liquid to more than the 90 DEG C enzymes that go out
10-20min, obtains giant salamander enzymolysis polypeptide liquid；3) micro-filtration：By step 2) gained giant salamander enzymolysis polypeptide liquid carried out through 0.45 μm of microfiltration membranes
Micro-filtration, takes filtrate；4) ultrafiltration：By step 3) gained filtrate using 5000 molecular weight milipore filter carry out ultrafiltration, obtain giant salamander polypeptide powder
Liquid, it is standby；5) first giant salamander polypeptide powder liquid, white granulated sugar, cider, citric acid, cycloheptaamylose and water are well mixed, add Huang
Virgin rubber solution, and be well mixed, by mass percentage, the ratio of each component is：Giant salamander polypeptide powder liquid 20-50%, white granulated sugar 8-
12%th, cider 3-5%, citric acid 0.01-0.03%, cycloheptaamylose 0.5-1%, xanthans 0.05-0.15%, surplus is
Pure water；6) by step 5) gains use 500 mesh filter-cloth filterings, then carry out filling；7) by step 6) gains progress height
Warm high pressure sterilization, sterilization mode is：Heat up 10min, in 110-115 DEG C, 0.07MPa sterilization 15-40min, classification cooling
10min, is cooled to central temperature less than 40 DEG C, produces giant salamander polypeptide powder Antioxidation Oral Liquid finished product；The complex enzyme, in mass ratio
Meter, is made up of following enzyme：Neutral proteinase:Papain:Flavor protease=2:1:1.
Publication No. CN104988199A China discloses a kind of preparation method of giant salamander mucus albumen peptide, 1) electric shock thorn
The method of swashing collection giant salamander mucus, freezes to obtain mucus freeze-dried powder；2) gained mucus freeze-dried powder is added to the distilled water of 50~120 times of quality
In, mucus freeze-dried powder quality 10%-30% alkali protease is added, pH to 9~9.5 is adjusted, in 40~60 DEG C of temperature strips
1~3h is digested under part, then mixed liquor is boiled into 1~2min, 19~25 DEG C are cooled to；3) by step 2) handle the mixed liquor completed
Under the conditions of 3000-4500r/min, 10~20min is centrifuged, clear liquid is taken, regulation clear liquid pH value is 4~5, in 40~45 DEG C of temperature
Under the conditions of, 1%~2% activated carbon is added, 45~60min of stirring carries out decoloration deodorization, then in 3000-4500r/min conditions
Under, 10-15min is centrifuged, 0.45 μm of filter membrane is crossed and obtains filtrate；4) by step 3) filtrate completed is handled in 30 DEG C of -60 DEG C of temperature strips
Rotation is concentrated by evaporation to as former state 1/7~1/8 under part, by concentrate plastic bag sealing, in 19~25 DEG C of temperature, pressure 150-
Under the conditions of 250MPa, 1-5min is sterilized, under clean environment, vacuum freeze drying, crushing obtain mucus protein Gly-His-Lys.
Publication No. CN105018555A China discloses a kind of preparation method of giant salamander collagen peptide, by giant salamander
Skin degreasing simultaneously carries out deodorant, then removes with alkali lye casting skin and foreign protein, then in 20~25 DEG C of temperature, pressure 150-300MPa conditions
10~60min of lower pressurize, the giant salamander skin pH to 8~10 that regulation ultra high pressure treatment is crossed, adds the alkalescence of cladding weight 2%~3%
Protease, under the conditions of 45~50 DEG C of temperature, lasting stirring enzymolysis 3~6 hours, then batch mixing is boiled into 1~2min, it is cooled to 40
DEG C, then batch mixing pH to 4~5 is adjusted, the activated carbon of batch mixing quality 1% is added, stirring is decolourized；The batch mixing completed will be handled in rotating speed
Under the conditions of 3000-4500r/min, centrifuge 10~15min, then successively through 0.45 μm of filter membrane micro-filtration, molecular cut off be 4~
5kD milipore filter ultrafiltration, molecular cut off obtain filtrate for 2-3kD NF membrane nanofiltration, then concentrate the filtrate to two points of former state
One of, concentrate is freezed and crushed produce giant salamander collagen Gly-His-Lys.It is small that the present invention prepares collagen peptide molecular weight,
More than 90% molecular weight is less than 1000 dalton.
Publication No. CN105399815A China discloses a kind of giant salamander collagen sugar peptide, is prepared as follows：A. with
Giant salamander skin is raw material, and the acid-soluble collagen of giant salamander skin is made；B. using the acid-soluble collagen of giant salamander skin as raw material, giant salamander is made
Collagen peptide；C. 10g oyster polysaccharides are added in 100ml0.01mol/LpH6.5 sodium phosphate buffer, stir 2h,
5g giant salamanders collagen peptide and 10g oyster polysaccharides are added, continues to stir 2h, 4 DEG C stand overnight, then 60 DEG C of stirring 4h, it is cooled to
Room temperature, 9000r/min centrifugation 30min obtain supernatant, freeze-drying supernatant is to obtain giant salamander gelatin raw sugar peptide.
Publication No. CN106107397A China discloses a kind of preparation method of giant salamander protein peptide powder solid beverage, bag
Include following steps：(1), by giant salamander protein peptide powder 2-5%, inulin 5-15%, dragon fruit 10-20%, Tea Polyphenols 2-5%, wood
Sugared 2-4%, honey powder 5-15%, local flavor blender 35-75% are added in blender and are well mixed, and obtain mixture；(2), will
The mixture that step (1) is obtained packed after ultraviolet sterilization and both obtained.
Although giant salamander protein content quite enriches, nutritional ingredient is better than other animal products, from above-mentioned document money
Expect to show to study more in terms of glycopeptide, related other functional components exploitations are quite weak, and nutrition in process
Composition loss is serious, and the exploitation added value of product is low, causes giant salamander added value low, constrains giant salamander industry development.
The content of the invention
The technical problems to be solved by the invention are：For the serious awkward situation of nutritive loss in existing giant salamander process,
What is provided short, giant salamander glycopeptide of a kind of rational technology, simple to operate, process cycle etc. nutrient loss is few, be of high nutritive value supports
The preparation method of raw giant salamander nutrient powder.
Therefore, first purpose of the invention be to provide it is a kind of prepare giant salamander nutrient powder product using radix polygonati officinalis, take polygonati rhizoma
20~40 parts, 10~15 parts of saponin rice, 40~60 parts of giant salamander, after high temperature flooding, enzymolysis, allotment, drying for standby.
Second purpose of the invention is to provide the preparation method of above-mentioned nutrient powder product, comprises the steps：
(1) take fresh giant salamander or giant salamander leftover bits and pieces directly to rub, after addition acetone stirs, stand 20~30min and go
Except upper strata acetone soln obtains insoluble matter, wherein it is 2~8L: 1kg to add the volume of acetone with the weight ratio of giant salamander raw material；
(2) insoluble matter adds water and suspended, and adds trypsase and 4~4.5h, wherein pancreas are hydrolyzed at 35~45 DEG C
The final concentration of protease reaches more than 300U/L, obtains enzymolysis liquid I, wherein add water volume and insoluble matter weight ratio for 3~
6L∶1kg；
(3) enzymolysis liquid I centrifuges 20~30min through 4000~8000rpm, obtains precipitation II and supernatant II；
(4) saponin rice of good quality is selected, be soaked in water 10~12h, gone after water in 20~30 DEG C of dark insulation vernalization 2~4
After it, add water and rub homogenate again, obtain saponin rice homogenate, wherein saponin rice weight and water volume ratio are 2~4：10L；
(5) precipitation II is added into saponin rice homogenate, neutral proteinase is added and 4~4.5h is hydrolyzed at 35~45 DEG C
Enzymolysis liquid II is obtained, the final concentration of wherein neutral proteinase reaches more than 100U/L；
(6) enzymolysis liquid II is mixed with supernatant II, is heated to 85~90 DEG C, be sufficiently stirred for 15~18min of lower insulation,
4000~8000rpm centrifuges 20~30min, obtains supernatant III.
(7) cut into slices from polygonati rhizoma of good quality, add a certain amount of water and mixed, carried out using refiner
20~50min of mechanical homogenisation, obtains radix polygonati officinalis homogenate, wherein volume L and polygonati rhizoma weight the kg ratio for adding water are 4~7：1；
(8) by radix polygonati officinalis homogenate add complex cellulase carry out enzymolysis 3~8h, 80~100 DEG C decoct 100~
After 150min, filtering, filtrate is mixed with supernatant III, and nutrient powder is concentrated to dryness to obtain on 60~75 DEG C, thin film evaporation concentrating instrument
Product.
In a specific embodiment, wherein step (6) obtain supernatant III can be directly as small peptide oral liquid
Use, dry use can also be directly thickened to or be added in other health products.
In one embodiment, the mechanical homogenisation used in step (7), is mainly carried out polygonati rhizoma under normal pressure
1000~2500rpm low speed is homogenized, and Homogenization time is 20~50min.
In one embodiment, the filter process of step (8) is filtered through gauze, preferably three layers filtered through gauze 2 times, micro-
Filter 1 time.
In one embodiment, the amount of complex cellulase described in step (8), make the final concentration of enzyme 5U/mL with
On.
Above-mentioned obtained various products can be applied in the free base product of removing is prepared, and can also be applied and prepared raising
In immunity of organisms product, it can be applied in preparation and promote in skin fibroblasts propagation product.
Technique effect
1st, the present invention takes full advantage of protein and polysaccharide and Flavonoid substances in giant salamander, radix polygonati officinalis and saponin rice, and gram
The fishlike smell that giant salamander produces during traditional handicraft has been taken, with invigorating the spleen moistening lung, beauty treatment, the effect such as enriching yin.
2nd, this method avoid the loss of giant salamander nutrient protein composition, by giant salamander by deep processings such as high-temperature process, enzymolysis
Processing, realizes the comprehensive process of giant salamander, process is easy to control, is easy to industrialized production, also increases giant salamander processing added value.
3rd, the method uses complex cellulase and protease hydrolytic, is conducive to fragrant solomonseal rhizome polyoses quick release, improves radix polygonati officinalis
Service efficiency, improves essential amino acids content in product.
4th, protein content in raw material is effectively increased by saponin rice germination treatment, grape in saponin rice is reduced again
Sugared content, amino acid content is improved by proteasome degradation, and polyphenoils in germination saponin rice can be made full use of again.
Embodiment
The essentiality content of the present invention is further illustrated with embodiments of the invention below, but this hair is not limited with this
It is bright.
Embodiment 1
Take the fresh giant salamanders of 160kg or giant salamander leftover bits and pieces directly to rub, after addition 500L acetone stirs, stand 25min
Remove upper strata acetone soln and obtain 140kg insoluble matters, insoluble matter adds 500L water and suspended, adds trypsase, make its end
Concentration reaches 400U/L, and 4h is hydrolyzed at 37 DEG C, obtains enzymolysis liquid I, and enzymolysis liquid I centrifuges 20~30min through 6000rpm, sunk
Form sediment II and supernatant II.
From 40kg saponin rices of good quality, be soaked in water 12h, goes after water after 26 DEG C are secretly incubated vernalization 3 days, addition
120L water rubs homogenate again, obtains saponin rice homogenate, and precipitation II is added into saponin rice homogenate, neutral proteinase is added,
Its final concentration is reached 150U/L, 4h is hydrolyzed at 40 DEG C and obtains enzymolysis liquid II, enzymolysis liquid II is mixed with supernatant II, is heated to
85 DEG C, lower insulation 18min, 6000rpm centrifugation 30min are sufficiently stirred for, supernatant III is obtained.
Cut into slices from 100kg polygonati rhizomas of good quality, the water for adding 500L is mixed, and is carried out using refiner
Low-speed machinery is homogenized 40min, obtains radix polygonati officinalis homogenate, adds complex cellulase, makes its final concentration in more than 10U/mL, enzymolysis
6h, after 90 DEG C decoct 120min, three layers of filtered through gauze 2 times, micro-filtration 1 time, filtrate is blended in 60~75 DEG C with supernatant III, thin
Film is concentrated by evaporation on instrument and is concentrated to dryness to obtain giant salamander nutrient powder product.
Claims (7)
1. preparing giant salamander nutrient powder product using radix polygonati officinalis, 20~40 parts of polygonati rhizoma, 10~15 parts of saponin rice, giant salamander 40~60 are taken
Part, after high temperature flooding, enzymolysis, allotment, drying for standby.
2. the method for claim 1, the preparation method of the giant salamander nutrient powder product, comprises the steps：
(1) take fresh giant salamander or giant salamander leftover bits and pieces directly to rub, after addition acetone stirs, stand in 20~30min removals
Layer acetone soln obtains insoluble matter, wherein it is 2~8L: 1kg to add the volume of acetone with the weight ratio of giant salamander raw material；
(2) insoluble matter adds water and suspended, and adds trypsase and 4~4.5h, wherein tryptose are hydrolyzed at 35~45 DEG C
The final concentration of enzyme reaches more than 300U/L, obtains enzymolysis liquid I, wherein it is 3~6L to add the volume of water and the weight ratio of insoluble matter:
1kg；
(3) enzymolysis liquid I centrifuges 20~30min through 4000~8000rpm, obtains precipitation II and supernatant II；
(4) saponin rice of good quality is selected, be soaked in water 10~12h, gone after water after 20~30 DEG C are secretly incubated vernalization 2~4 days,
Add water and rub homogenate again, obtain saponin rice homogenate, wherein saponin rice weight and water volume ratio are 2~4：10L；
(5) precipitation II is added into saponin rice homogenate, neutral proteinase is added 4~4.5h is hydrolyzed at 35~45 DEG C and obtain enzyme
Liquid II is solved, the final concentration of wherein neutral proteinase reaches more than 100U/L；
(6) enzymolysis liquid II is mixed with supernatant II, is heated to 85~90 DEG C, be sufficiently stirred for lower insulation 15~18min, 4000~
8000rpm centrifuges 20~30min, obtains supernatant III.
(7) cut into slices from polygonati rhizoma of good quality, add a certain amount of water and mixed, machinery is carried out using refiner
20~50min is homogenized, radix polygonati officinalis homogenate is obtained, wherein volume L and polygonati rhizoma weight the kg ratio for adding water are 4~7：1；
(8) radix polygonati officinalis homogenate is added into complex cellulase and carries out 3~8h of enzymolysis, 100~150min is decocted at 80~100 DEG C
Afterwards, filter, filtrate is mixed with supernatant III, and nutrient powder product is concentrated to dryness to obtain on 60~75 DEG C, thin film evaporation concentrating instrument.
3. the method for claim 2, wherein step (6) obtain supernatant III and can used directly as small peptide oral liquid,
Dry use can be directly thickened to or be added in other health products.
4. the mechanical homogenisation used in the method for claim 2, wherein step (7), is mainly carried out polygonati rhizoma under normal pressure
1000~2500rpm low speed is homogenized, and Homogenization time is 20~50min.
5. the filter process of the method for claim 2, wherein step (8) is filtered through gauze, preferably three layers filtered through gauze 2 times,
Micro-filtration 1 time.
6. the amount of complex cellulase described in the method for claim 2, wherein step (8), make the final concentration of enzyme 5U/mL with
On.
7. the method for claim 1~6, various products can be applied in the free base product of removing is prepared obtained in it,
It can apply in raising immunity of organisms product is prepared, can be applied in preparation and promote skin fibroblasts propagation product
In.
Priority Applications (1)
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