CN108484716A - A kind of extracting method of grub antibacterial peptide and its application - Google Patents
A kind of extracting method of grub antibacterial peptide and its application Download PDFInfo
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- CN108484716A CN108484716A CN201810190291.3A CN201810190291A CN108484716A CN 108484716 A CN108484716 A CN 108484716A CN 201810190291 A CN201810190291 A CN 201810190291A CN 108484716 A CN108484716 A CN 108484716A
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- grub
- antibacterial peptide
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a kind of extracting method of grub antibacterial peptide and its applications, additionally provide a kind of fish meal.The extracting method of the grub antibacterial peptide is substantially：It cleans worm living and injects cultured bacterium solution, homogenate is made after disinfection, filtering extracts it with filter membrane by ultrasound, is finally dried in vacuo to obtain required grub antibacterial peptide.The present invention can keep it is active under the premise of high efficiency extraction grub antibacterial peptide.
Description
Technical field
The present invention relates to a kind of extracting methods of antibacterial peptide, and in particular to a method of extracting antibacterial peptide from grub.
Background technology：
Grub also known as hen worm, grub, one's old mother worm, worm etc. when white, build is loose, common name scarab beetle or cockchafer after adult
Son, composition are mainly made of fat, protein.
For insect as the more huge species of population quantity type on the earth, insect possesses unique and larger animal not
The same immunologic mechanism carries out immune defense by secreting the various immune substances including antibacterial peptide, therefore passes through induction
Form promote Insect transformation secret out of all kinds of antibacterial peptides, by its extraction and application, curative effect more preferable than conventional medicament can be obtained
Product.
Antibacterial peptide is small molecule peptides active material, is the small peptide with antibacterial activity, is present in different kind organism body
It is interior.Antibacterial peptide has certain resistance to microorganism and parasite, but is influenced on organism itself smaller or without influence, and arrives mesh
Before until not yet find that antibacterial peptide can make microorganism and parasite generate resistance, be that one kind can be used for substituting resistance getting worse
Antibiotic product.
About the extraction application of grub antibacterial peptide, report is less, and traditional extraction process is that directly mashing is obtained by filtration, pure
Degree and content are insufficient.
Invention content：
The method that the object of the present invention is to provide a kind of to extract antibacterial peptide from grub, to solve the above technical problems.
The present invention can keep it is active under the premise of high efficiency extraction grub antibacterial peptide.
To achieve the above object, the present invention provides following scheme：
The present invention provides a kind of method for extracting antibacterial peptide from grub, includes the following steps：
(1) fresh grub is captured, is cleaned spare；
(2) by Escherichia coli and bacillus subtilis overnight incubation in the medium, according to 1:The bacterium solution of 3~5 volume ratios
Mixing, thalline were collected by centrifugation, is resuspended and is precipitated with sterile saline, and as induction source, mixed bacteria liquid is dipped with transfer needle, light to pierce
The grub abdomen for entering step (1), continues 1~3d of raising；
(3) grub after inducing step (2) is placed in 75~80% alcohol after immersion 10s, is remained with aseptic water washing
Alcohol, be fully ground into homogenate；
(4) homogenate of step (3) is taken, the extracting solution of 10~20 times of volumes is added, then 40~60min of ultrasonic extraction, mistake
Filtrate is collected in filter；
(5) filtrate of step (4) is removed into big molecular impurity with ultrafiltration membrane, collects ultrafiltration permeate；
(6) the ultrafiltration permeate of step (5) NF membrane is removed into small molecular weight impurity, collects nanofiltration retentate fluid；
(7) nanofiltration retentate fluid of step (6) is dried in vacuo at 40~50 DEG C, obtains grub antibacterial peptide.
Further, extracting solution is 0.05mol/L ammonium acetate buffers, 35 μ g/ml benzyl sulfonyls in the step (4)
Fluorine, 1mg/ml phenylthioureas in proportion 3~5:1:The mixed liquor of 1 composition.
Further, ultrasound condition in the step (4)：Power is 150~250W, and frequency is 40~50kHz.
Further, ultrafiltration retaining molecular weight is 10000~12000Da in the step (5).
Further, nanofiltration retaining molecular weight is 200~250Da in the step (6).
Further, the grub antibacterial peptide that said extracted obtains can apply in feed.
Further, the feed is fish meal.
Further, the present invention also provides a kind of fish meals, include the ingredient of following parts by weight：Grub antibacterial peptide 5~10
Part, 10~20 parts of dregs of beans, 10~20 parts of rapeseed meal, 1.2~2.5 parts of multi-vitamins, 1~3 part of cellulase, pectase 1~3
Part.
Compared with prior art, the present invention has following advantageous effect：
(1) it is added to phenylmethylsulfonyl fluoride in extraction process in the present invention, can be prevented by the decomposition of inhibition antibacterial peptide
Therefore the antibacterial peptide yield rate generated reduces and quality decline；It is also added to phenylthiourea simultaneously, it can be by increasing protein
Solubility property, to increase the recovery rate of antibacterial peptide；
(2) extracting method of the invention uses membrane separation technique, is extracted, is avoided damage to using physical separating process
The activity of antibacterial peptide keeps the antibacterial peptide purity higher of acquisition, quality more preferable；
(3) grub antibacterial peptide can be applied in fish meal, improve the immune of fish by antibacterial peptide, reduce morbidity, reach
Indirect growth promotion and the effect for improving finished product fish products matter.
Specific implementation mode
With reference to specific embodiment, the present invention is described in further detail.
Embodiment 1
A method of it extracting antibacterial peptide from grub, includes the following steps：
(1) fresh grub is captured, is cleaned spare；
(2) by Escherichia coli and bacillus subtilis overnight incubation in the medium, according to 1:The bacterium solution of 4 volume ratios is mixed
It closes, thalline were collected by centrifugation, is resuspended and is precipitated with sterile saline, and as induction source, mixed bacteria liquid is dipped with transfer needle, light to be pierced into
The grub abdomen of step (1) continues to raise 2d；
(3) grub after inducing step (2) is placed in 78% alcohol after immersion 10s, with the remaining wine of aseptic water washing
Essence is fully ground into homogenate；
(4) homogenate of step (3) is taken, the extracting solution of 15 times of volumes is added, extracting solution is 0.05mol/L ammonium acetate buffers
Liquid, 35 μ g/ml phenylmethylsulfonyl fluorides, 1mg/ml phenylthioureas in proportion 4:1:1 composition mixed liquor, be then in power
200W, frequency are ultrasonic extraction 50min under conditions of 45kHz, and filtrate is collected in filtering；
(5) filtrate of step (4) is removed into big molecular impurity with the ultrafiltration membrane that molecular cut off is 10000Da, collected super
Filter permeate；
(6) the ultrafiltration permeate of step (5) is removed into small molecular weight impurity with the NF membrane that molecular cut off is 250Da, received
Collect nanofiltration retentate fluid；
(7) nanofiltration retentate fluid of step (6) is dried in vacuo at 45 DEG C, obtains grub antibacterial peptide.
Embodiment 2
A method of it extracting antibacterial peptide from grub, includes the following steps：
(1) fresh grub is captured, is cleaned spare；
(2) by Escherichia coli and bacillus subtilis overnight incubation in the medium, according to 1:The bacterium solution of 3 volume ratios is mixed
It closes, thalline were collected by centrifugation, is resuspended and is precipitated with sterile saline, and as induction source, mixed bacteria liquid is dipped with transfer needle, light to be pierced into
The grub abdomen of step (1) continues to raise 3d；
(3) grub after inducing step (2) is placed in 75% alcohol after immersion 10s, with the remaining wine of aseptic water washing
Essence is fully ground into homogenate；
(4) homogenate of step (3) is taken, the extracting solution of 10 times of volumes is added, extracting solution is 0.05mol/L ammonium acetate buffers
Liquid, 35 μ g/ml phenylmethylsulfonyl fluorides, 1mg/ml phenylthioureas in proportion 3:1:1 composition mixed liquor, be then in power
250W, frequency are ultrasonic extraction 40min under conditions of 40kHz, and filtrate is collected in filtering；
(5) filtrate of step (4) is removed into big molecular impurity with the ultrafiltration membrane that molecular cut off is 12000Da, collected super
Filter permeate；
(6) the ultrafiltration permeate of step (5) is removed into small molecular weight impurity with the NF membrane that molecular cut off is 250Da, received
Collect nanofiltration retentate fluid；
(7) nanofiltration retentate fluid of step (6) is dried in vacuo at 40 DEG C, obtains grub antibacterial peptide.
Embodiment 3
A method of it extracting antibacterial peptide from grub, includes the following steps：
(1) fresh grub is captured, is cleaned spare；
(2) by Escherichia coli and bacillus subtilis overnight incubation in the medium, according to 1:The bacterium solution of 5 volume ratios is mixed
It closes, thalline were collected by centrifugation, is resuspended and is precipitated with sterile saline, and as induction source, mixed bacteria liquid is dipped with transfer needle, light to be pierced into
The grub abdomen of step (1) continues to raise 1d；
(3) grub after inducing step (2) is placed in 80% alcohol after immersion 10s, with the remaining wine of aseptic water washing
Essence is fully ground into homogenate；
(4) homogenate of step (3) is taken, the extracting solution of 20 times of volumes is added, extracting solution is 0.05mol/L ammonium acetate buffers
Liquid, 35 μ g/ml phenylmethylsulfonyl fluorides, 1mg/ml phenylthioureas in proportion 5:1:1 composition mixed liquor, be then in power
150W, frequency are ultrasonic extraction 60min under conditions of 50kHz, and filtrate is collected in filtering；
(5) filtrate of step (4) is removed into big molecular impurity with the ultrafiltration membrane that molecular cut off is 10000Da, collected super
Filter permeate；
(6) the ultrafiltration permeate of step (5) is removed into small molecular weight impurity with the NF membrane that molecular cut off is 200Da, received
Collect nanofiltration retentate fluid；
(7) nanofiltration retentate fluid of step (6) is dried in vacuo at 50 DEG C, obtains grub antibacterial peptide.
Embodiment 4
A kind of fish meal, it is specific such as table 1.
Table 1：
| Fish meal | Parts by weight |
| Grub antibacterial peptide | 7.5 |
| Dregs of beans | 15 |
| Rapeseed meal | 15 |
| Multi-vitamins | 1.8 |
| Cellulase | 2.0 |
| Pectase | 2.0 |
Embodiment 5
A kind of fish meal, it is specific such as table 2.
Table 2：
| Fish meal | Parts by weight |
| Grub antibacterial peptide | 8.0 |
| Dregs of beans | 18 |
| Rapeseed meal | 13 |
| Multi-vitamins | 2.0 |
| Cellulase | 2.5 |
| Pectase | 1.5 |
Comparative example 1
A method of it extracting antibacterial peptide from grub, includes the following steps：
(1) fresh grub is captured, is cleaned spare；
(2) by Escherichia coli and bacillus subtilis overnight incubation in the medium, according to 1:The bacterium solution of 4 volume ratios is mixed
It closes, thalline were collected by centrifugation, is resuspended and is precipitated with sterile saline, and as induction source, mixed bacteria liquid is dipped with transfer needle, light to be pierced into
The grub abdomen of step (1) continues to raise 2d；
(3) grub after inducing step (2) is placed in 78% alcohol after immersion 10s, with the remaining wine of aseptic water washing
Essence is fully ground into homogenate；
(4) homogenate of step (3) is taken, the extracting solution of 15 times of volumes is added, extracting solution is 0.05mol/L ammonium acetate buffers
Liquid, 1mg/ml phenylthioureas in proportion 4:1:Then the mixed liquor of 1 composition is 200W in power, under conditions of frequency is 45kHz
Filtrate is collected in ultrasonic extraction 50min, filtering；
(5) filtrate of step (4) is removed into big molecular impurity with the ultrafiltration membrane that molecular cut off is 10000Da, collected super
Filter permeate；
(6) the ultrafiltration permeate of step (5) is removed into small molecular weight impurity with the NF membrane that molecular cut off is 250Da, received
Collect nanofiltration retentate fluid；
(7) nanofiltration retentate fluid of step (6) is dried in vacuo at 45 DEG C, obtains grub antibacterial peptide.
Comparative example 2
A method of it extracting antibacterial peptide from grub, includes the following steps：
(1) fresh grub is captured, is cleaned spare；
(2) by Escherichia coli and bacillus subtilis overnight incubation in the medium, according to 1:The bacterium solution of 4 volume ratios is mixed
It closes, thalline were collected by centrifugation, is resuspended and is precipitated with sterile saline, and as induction source, mixed bacteria liquid is dipped with transfer needle, light to be pierced into
The grub abdomen of step (1) continues to raise 2d；
(3) grub after inducing step (2) is placed in 78% alcohol after immersion 10s, with the remaining wine of aseptic water washing
Essence is fully ground into homogenate；
(4) homogenate of step (3) is taken, the 0.05mol/L ammonium acetate buffers of 15 times of volumes are added, are then in power
200W, frequency are ultrasonic extraction 50min under conditions of 45kHz, and filtrate is collected in filtering；
(5) filtrate of step (4) is removed into big molecular impurity with the ultrafiltration membrane that molecular cut off is 10000Da, collected super
Filter permeate；
(6) the ultrafiltration permeate of step (5) is removed into small molecular weight impurity with the NF membrane that molecular cut off is 250Da, received
Collect nanofiltration retentate fluid；
(7) nanofiltration retentate fluid of step (6) is dried in vacuo at 45 DEG C, obtains grub antibacterial peptide.
Comparative example 3
A method of it extracting antibacterial peptide from grub, includes the following steps：
(1) fresh grub is captured, is cleaned spare；
(2) by Escherichia coli and bacillus subtilis overnight incubation in the medium, according to 1:The bacterium solution of 4 volume ratios is mixed
It closes, thalline were collected by centrifugation, is resuspended and is precipitated with sterile saline, and as induction source, mixed bacteria liquid is dipped with transfer needle, light to be pierced into
The grub abdomen of step (1) continues to raise 2d；
(3) grub after inducing step (2) is placed in 78% alcohol after immersion 10s, with the remaining wine of aseptic water washing
Essence is fully ground into homogenate；
(4) homogenate of step (3) is taken, the extracting solution of 15 times of volumes is added, extracting solution is 0.05mol/L ammonium acetate buffers
Liquid, 1mg/ml phenylthioureas in proportion 4:1:Then the mixed liquor of 1 composition is 200W in power, under conditions of frequency is 45kHz
Filtrate is collected in ultrasonic extraction 50min, filtering；
(5) filtrate of step (4) is filtered, collects filtrate and be dried in vacuo at 45 DEG C, obtains grub antibacterial peptide.
Grub antibacterial peptides extraction rate is evaluated
Preferably to prove that it is excellent that a kind of grub antibacterial peptide extracting method provided by the present invention has the effect of, choose real
It applies example 1,2,3 and the extracting method of comparative example 1,2,3 extracts grub antibacterial peptide respectively, following table is carried out to grub antibacterial peptides extraction rate
The measurement.
Table 3：The performance test results of embodiment 1-3 and comparative example 1-3：
The result shows that：
From table 3 it is observed that the grub antibacterial peptide extracted by the grub antibacterial peptide extracting method of the present invention carries
It takes rate higher, and is better than comparative example.The comparative example 1 of phenylmethylsulfonyl fluoride is not added compared with Example 1, grub antibacterial peptide carries
It takes rate relatively low, shows that phenylmethylsulfonyl fluoride, which is added, can help the extraction of grub antibacterial peptide；Phenylmethylsulfonyl fluoride, phenyl are not added
Compared with Example 1, grub antibacterial peptides extraction rate is relatively low for the comparative example 2 of thiocarbamide, shows that phenylmethylsulfonyl fluoride, phenylthiourea is added
It can help the extraction of grub antibacterial peptide；Compared with Example 1, grub antibacterial peptide obtains the comparative example 3 of unused membrane separation technique
Rate is significant lower, shows to use membrane separation technique, be extracted using physical separating process, avoids damage to the work of antibacterial peptide
Property, keep the antibacterial peptide purity higher of acquisition, quality more preferable.
Immunization is evaluated
The grub antibacterial peptide of embodiment 1-2 and the extraction of 1 preparation method of comparative example, the fish meal provided according to embodiment 4 are matched
Fang Jinhang tries out detection result；The grub antibacterial peptide of embodiment 3 and the extraction of comparative example 2-3 preparation methods, provides according to embodiment 5
Fish feed formula carry out detection result on probation；Simultaneously using a commercially available fish meal as control group.
Test object：Each experimental group is 50 goldfish, cardinal symptom be do not eat food, do not like trip, it is slow in action, irritable not
Peace etc..The feed organized where all daily uses of goldfish, test period is 15 days, and tested fish symptom was tested at the 15th day.It is real
Other any feeds must not be eaten during testing, and tested goldfish has been fully completed verification process, experimental result is shown in Table 4.
Table 4：The performance test results of embodiment 1-3 and comparative example 1-3, control group：
Disease resistance is evaluated
The grub antibacterial peptide of embodiment 1-2 and the extraction of 1 preparation method of comparative example, the fish meal provided according to embodiment 4 are matched
Fang Jinhang tries out detection result；The grub antibacterial peptide of embodiment 3 and the extraction of comparative example 2-3 preparation methods, provides according to embodiment 5
Fish feed formula carry out detection result on probation；Simultaneously using a commercially available fish meal as control group.
Table 5：The performance test results of embodiment 1-3 and comparative example 1-3, control group：
The result shows that：
As can be seen from Table 5, the grub antibacterial peptide immunity energy extracted by the grub antibacterial peptide extracting method of the present invention
Power is higher, and is better than comparative example.As can be seen from Table 5, the Qi extracted by the grub antibacterial peptide extracting method of the present invention
Scarabaeiform antibacterial peptide disease resistance is higher, and is better than comparative example.Show to apply grub antibacterial peptide in fish meal, passes through antibacterial peptide
The immune of fish is improved, morbidity is reduced, have the function that indirect growth promotion and improves finished product fish products matter.
The present invention is described by way of example above, but the present invention is not limited to above-mentioned specific embodiment, all to be based on
Any changes or modifications that the present invention is done belong to the scope of protection of present invention.
Claims (8)
1. a kind of extracting method of grub antibacterial peptide, which is characterized in that include the following steps：
(1) fresh grub is captured, is cleaned spare；
(2) by Escherichia coli and bacillus subtilis overnight incubation in the medium, according to 1:The bacterium solution of 3~5 volume ratios mixes,
Thalline were collected by centrifugation, is resuspended and is precipitated with sterile saline, and as induction source, mixed bacteria liquid is dipped with transfer needle, light to be pierced into step
Suddenly the grub abdomen of (1), continues 1~3d of raising；
(3) grub after inducing step (2) is placed in 75~80% alcohol after immersion 10s, with the remaining wine of aseptic water washing
Essence is fully ground into homogenate；
(4) homogenate of step (3) is taken, the extracting solution of 10~20 times of volumes is added, then 40~60min of ultrasonic extraction, is filtered, is received
Collect filtrate；
(5) filtrate of step (4) is removed into big molecular impurity with ultrafiltration membrane, collects ultrafiltration permeate；
(6) the ultrafiltration permeate of step (5) NF membrane is removed into small molecular weight impurity, collects nanofiltration retentate fluid；
(7) nanofiltration retentate fluid of step (6) is dried in vacuo at 40~50 DEG C, obtains grub antibacterial peptide.
2. extracting method according to claim 1, which is characterized in that extracting solution is 0.05mol/L second in the step (4)
Sour ammonium buffer solution, 35 μ g/ml phenylmethylsulfonyl fluorides, 1mg/ml phenylthioureas in proportion 3~5:1:The mixed liquor of 1 composition.
3. extracting method according to claim 1, which is characterized in that ultrasound condition in the step (4)：Power is 150
~250W, frequency are 40~50kHz.
4. extracting method according to claim 1, which is characterized in that ultrafiltration retaining molecular weight is in the step (5)
10000~12000Da.
5. extracting method according to claim 1, which is characterized in that nanofiltration retaining molecular weight is in the step (6)
200~250Da.
6. according to application of the grub antibacterial peptide in feed made from any one of Claims 1 to 5 extracting method.
7. application according to claim 6, which is characterized in that the feed is fish meal.
8. a kind of fish meal, which is characterized in that include the ingredient of following parts by weight：The grub as described in any one of Claims 1 to 5
5~10 parts of antibacterial peptide, 10~20 parts of dregs of beans, 10~20 parts of rapeseed meal, 1.2~2.5 parts of multi-vitamins, 1~3 part of cellulase,
1~3 part of pectase.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810190291.3A CN108484716A (en) | 2018-03-07 | 2018-03-07 | A kind of extracting method of grub antibacterial peptide and its application |
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|---|---|---|---|
| CN201810190291.3A CN108484716A (en) | 2018-03-07 | 2018-03-07 | A kind of extracting method of grub antibacterial peptide and its application |
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|---|---|
| CN108484716A true CN108484716A (en) | 2018-09-04 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110272467A (en) * | 2019-07-23 | 2019-09-24 | 周口师范学院 | A kind of concentration purifying plant of antibacterial peptide |
| CN111334547A (en) * | 2020-02-15 | 2020-06-26 | 松颉环保科技(深圳)有限公司 | Extraction method of hermetia illucens antibacterial peptide and application of hermetia illucens antibacterial peptide in preparation of tobacco disease resistant fertilizer |
-
2018
- 2018-03-07 CN CN201810190291.3A patent/CN108484716A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110272467A (en) * | 2019-07-23 | 2019-09-24 | 周口师范学院 | A kind of concentration purifying plant of antibacterial peptide |
| CN111334547A (en) * | 2020-02-15 | 2020-06-26 | 松颉环保科技(深圳)有限公司 | Extraction method of hermetia illucens antibacterial peptide and application of hermetia illucens antibacterial peptide in preparation of tobacco disease resistant fertilizer |
| CN111334547B (en) * | 2020-02-15 | 2022-03-08 | 松颉环保科技(深圳)有限公司 | Extraction method of hermetia illucens antibacterial peptide and application of hermetia illucens antibacterial peptide in preparation of tobacco disease resistant fertilizer |
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Application publication date: 20180904 |