CN103160392A - Method for preparing non-toxic fruit and vegetable detergent - Google Patents

Abstract

The invention discloses a method for preparing a non-toxic fruit and vegetable detergent, and belongs to the technical field of civilian industry. The main points of the technical program include that: bean curd seriflux which is the waste water discharged in the production processes of bean products such as bean curd, dried bean curd, thin sheets of bean curd and the like is used as a raw material, the active ingredients are isolated by centrifugation, ethanol precipitation and other simple and environmental-friendly technologies, then dissolved with a small amount of water, added with an appropriate amount of salt and sodium carboxymethyl cellulose, and evenly mixed to get the detergent. The detergent can effectively wash away pesticide residues on fruits and vegetables, has a good sterilization and antibacterial effect, and is harmless to humans and animals.

Description

A kind of preparation method of nontoxic fruits and vegetables washing composition

Technical field

The invention belongs to civilian chemical technology field.Especially relate to a kind of method of utilizing the soya-bean milk water washing to remove remains of pesticide on fruit and vegetable.

Background technology

The residual toxicity of agricultural chemicals is brought serious pollution problem to the agricultural-food such as vegetables and environment, and the production phase of vegetables is shorter, and the residual toxicity problem of this respect is just more outstanding.Current, the production of the agricultural-food such as grain, vegetables, fruit is to be unable to do without agricultural chemicals basically, and pesticide residue not only can cause acute poisoning, and larger harm is the agricultural chemicals chronic poisoning.Agricultural chemicals slowly corrodes organ by accumulating for a long time in vivo, finally brings out the various diseases such as cancer.In addition, the reason such as, fertilizer unclean due to irrigation water quality sterilization is ineffective, vegetables suffer the pollution of pathogenic bacterium also very serious.According to certain city's investigation, field of vegetables irrigation water detected result is shown, bacterial count is up to 6.5 * 10 ⁹Individual/ml, coliform count is 2.4 * 10 ⁵Individual/ml.Therefore, for ensureing food sanitation safe, be badly in need of a kind of washing composition with removing pesticide residue and pathogenic bacterium effect of exploitation, the washing composition of selling in the market is of a great variety, and great majority are soapless soaps synthetic take oil as raw material.The pollution that soapless soap causes ecotope and the murder by poisoning that immunologic function, reproductive function, neural system and the skin tissue of human body produces has been caused catches people's attention.

The bean curd pulp-water is the waste water in the bean product production processes such as bean curd, dried bean curd, the skin of soya-bean milk, also referred to as yellow seriflux.According to the study, in yellow seriflux, the content of general solid substance is more than 1%, and wherein protein content is about 0.3%, lipid content is about 0.08%, reducing sugar content is 0.15% left and right; In addition, also contain the several functions compositions such as soybean isoflavones, soybean saponin, China's most enterprises directly discharges yellow seriflux at present, does not only take full advantage of its useful component, and direct exhaust emission environment, be unfavorable for cleaner production and increase the soyabean processing added value.

The present invention take the bean curd pulp-water as raw material prepares nontoxic fruits and vegetables washing composition, can effectively wash pesticide residue and pathogenic bacterium on fruits and vegetables, become Fei Weibao, namely reduced the directly environmental pollution that causes of discharging of yellow seriflux, can increase again the edible security of fruits and vegetables.

Summary of the invention

The purpose of this invention is to provide a kind of low production cost, technique is simple, pollution-free, the preparation method of nontoxic fruits and vegetables washing composition effective, easy to use.

Comprise the steps:

The bean curd pulp-water that (1) will take out from the bean curd mill naturally cools to room temperature, and throw out is removed in first centrifugation.

(2) add ethanol to make its alcohol concn reach 60～80, stir evenly, standing alcohol precipitation spends the night, and after supernatant liquor is limpid, adopts siphon, centrifugal method separation of supernatant and throw out.

(3) with water-soluble open of throw out with 10 times of amounts of volume left and right, then slowly add while stirring salt and ethanol as sanitas.The consumption of salt is the 5-10% of the consumption of water, preferably 7.5% left and right.The consumption of ethanol is no more than 20% of the water yield, is preferably in 15% left and right.

(4) add carboxymethyl cellulose, add-on is too much unsuitable, and solution is got final product than thickness, stirs evenly to get final product.The rear washing of the water dilution fruits and vegetables that add decades of times during use.

(5) the alcohol precipitation supernatant liquor of above-mentioned siphon, after ethanol was reclaimed in underpressure distillation, high speed centrifugation separated the throw out that obtains under the rotating speed of 2000～4000 rev/mins, and is dry through negative-pressure vacuum, sieves to get the soybean isoflavones powder after pulverizing.This is byproduct.

The present invention has following advantage: low production cost, technique are simple, pollution-free, effective, easy to use.

Specific embodiment

Embodiment 1

Get bean curd pulp-water 1000ml, throw out is removed in the first centrifugation of bean curd pulp-water, then add the ethanol of two volumes to make its alcohol concn reach 66% left and right, stir evenly, standing alcohol precipitation spends the night, after supernatant liquor is limpid, and first siphon sucking-off supernatant liquor, separate remainder with centrifugal method again, isolate supernatant liquor and throw out.Throw out is made the aqueous solution with the water of 10 times of volumes, more slowly add while stirring 8g salt and 15ml ethanol, then slowly add while stirring the 0.5g carboxymethyl cellulose, stir evenly and be washing composition.Get water-soluble the opening as washings of 30 times of 10ml detergent uses.

Get appropriate commercially available SD-1750 and 'Miduowei ' agricultural chemical, be mixed into the agricultural chemicals mixed solution by after operation instruction dilution, get a string grape of uniform size and be put in the agricultural chemicals mixed solution and soak 5min, dry after taking-up.Therefrom appoint and get a grape and soak 10min with washings, dry as washing sample to be measured (being designated as the 1# sample).Get arbitrarily again a sample as 100% pesticide residue (being designated as the 0# sample) from the grape that agricultural chemicals soaked.

Ratio in 1g sample 5ml extracting solution (aqueous phosphatic) is placed in small beaker, place 10min, pull sample out, therefrom get 2.5ml and put into reaction flask as liquid to be measured, get the 2.5ml extracting solution and put into another reaction flask liquid in contrast, (contrast liquid, liquid to be measured) adds enzyme (acetylcholinesterase) 0.1ml in order, shake up 37 ℃ of constant temperature culture 10min.(contrast liquid in order, liquid to be measured) add again developer (DTNB) 0.1ml, add again substrate (acetylthiocholine iodide) 0.1ml, reaction flask is inverted (is not rocked for twice, in order to avoid produce bubble), be transferred to immediately in cuvette, put into the residual tacheometer of agriculture, press the detection key and test.

Inhibiting rate calculates: the residual tacheometer of agriculture can be measured the absorbancy before and after 3 minutes, and can automatically calculate inhibiting rate, and calculating formula is as follows:

Δ A in formula _CAfter expression contrast liquid 3min with 3min before absorbancy poor, Δ A _sRepresent after liquid 3min to be measured with 3min before absorbancy poor.

Test result is: the inhibiting rate of 0# sample (agriculture is residual is 100%) is 96%, and the inhibiting rate of 1# sample is 31%, and the residual clearance of agriculture is 68%.Illustrate that this washing composition can effectively remove the pesticide residue on fruits and vegetables.

Embodiment 2

Buy commercially available grape a string, appoint and to get one with the washings washing 10min of preparation in embodiment 1, get again a grape in contrast, two grapes are seeded in respectively on two albumen nutrient agars, put into constant incubator, 37 ℃ of lower constant temperature culture 24 hours, observe, the colony number on substratum relatively.

Result is not wash on the substratum of grape inoculation 4 bacterium colonies are arranged, and does not find bacterium colony on the substratum of the grape that washings was washed inoculation.This illustrates that this washing composition can wash the harmful bacteria on fruits and vegetables off.

Embodiment 3

Put into water in fish jar, and add the washing composition of configuration in 1/5000 embodiment 1, put into 3 goldfish, observed 3 days.Find that goldfish all lives.And adding 1/5000 general washing composition, goldfish is unlikely to survive half an hour.Illustrate that this washing composition is nontoxic.

Claims (7)

1. the preparation method of a nontoxic fruits and vegetables washing composition is characterized in that:

(1) preparation of this fruits and vegetables washing composition is that the waste water that discharges in the bean product production processes such as bean curd, dried bean curd, the skin of soya-bean milk-bean curd pulp-water is as raw material;

(2) method of making detergent is: first throw out is removed in the centrifugation on high-speed centrifuge of bean curd pulp-water, kept the supernatant pulp-water;

Then add a certain amount of ethanol in the supernatant pulp-water, fully stir evenly rear alcohol precipitation;

After hold over night, siphon supernatant liquor, throw out be with a small amount of water-soluble opening, then add a small amount of salt, ethanol and Xylo-Mucine, stirs to get final product.

2. as the raw material in claim explanation 1, can be the bright pulp-water when producing bean product, can be also the pulp-water of stored under refrigeration, unrestricted.

3. the alcohol concn during as the ethanol alcohol precipitation in claim explanation 1 more than 50%, is preferably in more than 60% unrestricted.

As the pure hypostasis in claim explanation 1 with water-soluble opening on a small quantity, the consumption of water is approximately 10 times of left and right of pure hypostasis, and is as long as can make uniform turbid solution, more also passable, unrestricted.

5. as the consumption of the salt in claim explanation 1 getting final product below 10% at the consumption of water.

6. the consumption as the last ethanol in claim explanation 1 is no more than 20% of the water yield.

7. the consumption as the Xylo-Mucine in claim explanation 1 is can form uniform and stable viscous fluid to get final product.