CN104957448B - A kind of method of cadmium in molten combination fermentation removing rice of acid - Google Patents
A kind of method of cadmium in molten combination fermentation removing rice of acid Download PDFInfo
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- CN104957448B CN104957448B CN201510453076.4A CN201510453076A CN104957448B CN 104957448 B CN104957448 B CN 104957448B CN 201510453076 A CN201510453076 A CN 201510453076A CN 104957448 B CN104957448 B CN 104957448B
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- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 157
- 235000009566 rice Nutrition 0.000 title claims abstract description 157
- 229910052793 cadmium Inorganic materials 0.000 title claims abstract description 90
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 title claims abstract description 84
- 239000002253 acid Substances 0.000 title claims abstract description 46
- 238000000855 fermentation Methods 0.000 title claims abstract description 34
- 230000004151 fermentation Effects 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 title claims abstract description 32
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000209094 Oryza Species 0.000 claims abstract description 156
- 235000012054 meals Nutrition 0.000 claims abstract description 85
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 38
- 239000008367 deionised water Substances 0.000 claims abstract description 32
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 32
- 239000000725 suspension Substances 0.000 claims abstract description 29
- 230000018044 dehydration Effects 0.000 claims abstract description 26
- 238000006297 dehydration reaction Methods 0.000 claims abstract description 26
- 241000191996 Pediococcus pentosaceus Species 0.000 claims abstract description 17
- 238000002156 mixing Methods 0.000 claims abstract description 16
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 14
- 230000010355 oscillation Effects 0.000 claims abstract description 14
- 238000005119 centrifugation Methods 0.000 claims abstract description 10
- 238000005406 washing Methods 0.000 claims abstract description 3
- 239000002002 slurry Substances 0.000 claims description 28
- 241000894006 Bacteria Species 0.000 claims description 26
- 238000004140 cleaning Methods 0.000 claims description 17
- 239000012530 fluid Substances 0.000 claims description 13
- 235000012149 noodles Nutrition 0.000 claims description 11
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 9
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 8
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 8
- 238000007605 air drying Methods 0.000 claims description 6
- 239000002054 inoculum Substances 0.000 claims description 6
- 229960000583 acetic acid Drugs 0.000 claims description 4
- 239000012362 glacial acetic acid Substances 0.000 claims description 4
- 239000004310 lactic acid Substances 0.000 claims description 4
- 235000014655 lactic acid Nutrition 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 claims description 3
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 claims description 3
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 239000001630 malic acid Substances 0.000 claims description 3
- 235000011090 malic acid Nutrition 0.000 claims description 3
- 239000011975 tartaric acid Substances 0.000 claims description 3
- 235000002906 tartaric acid Nutrition 0.000 claims description 3
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 claims description 2
- 235000015165 citric acid Nutrition 0.000 claims description 2
- 229910001385 heavy metal Inorganic materials 0.000 abstract description 18
- 238000006243 chemical reaction Methods 0.000 abstract description 3
- 238000012545 processing Methods 0.000 abstract description 3
- 230000004913 activation Effects 0.000 abstract 1
- WLZRMCYVCSSEQC-UHFFFAOYSA-N cadmium(2+) Chemical compound [Cd+2] WLZRMCYVCSSEQC-UHFFFAOYSA-N 0.000 abstract 1
- 238000007873 sieving Methods 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 8
- 235000013965 Lactobacillus plantarum Nutrition 0.000 description 6
- 235000013305 food Nutrition 0.000 description 6
- 229940072205 lactobacillus plantarum Drugs 0.000 description 6
- 238000012360 testing method Methods 0.000 description 5
- 238000007654 immersion Methods 0.000 description 4
- 150000007524 organic acids Chemical class 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 229920002472 Starch Polymers 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 235000019698 starch Nutrition 0.000 description 3
- 239000008107 starch Substances 0.000 description 3
- 238000009825 accumulation Methods 0.000 description 2
- 210000000481 breast Anatomy 0.000 description 2
- 239000010903 husk Substances 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 229940100486 rice starch Drugs 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 241000186660 Lactobacillus Species 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 210000000003 hoof Anatomy 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 150000004965 peroxy acids Chemical class 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 210000004994 reproductive system Anatomy 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/41—Pediococcus
- A23V2400/427—Pentosaceus
Abstract
A kind of method of cadmium in molten combination fermentation removing rice of acid, this method is that the exceeded rice of cadmium content pulverizes and sieves, then the rice meal by acquisition of sieving adds acid solution by a certain percentage, constant temperature oscillation processing is carried out after mixing, rice meal after acid soak is washed by deionized water and centrifugal dehydration, the preliminary cadmium for removing rice middle reaches and separating out；Deionized water is added in the rice meal after centrifugation, and by the lactobacillus plantarum bacteria suspension after activation and the access of Pediococcus pentosaceus bacteria suspension wherein, constant temperature is left to ferment, rice meal after fermentation is again through deionized water washing and centrifugal dehydration, further remove the cadmium ion that rice middle reaches separate out, finally dewatered rice meal is dried, the rice meal product significantly cut down up to cadmium content.Using this method, the removal efficiency of cadmium is up to more than 90% in rice, can not only effectively remove the heavy metal cadmium in rice, moreover it is possible to provides certain technical support for the safety conversion of " cadmium rice ".
Description
Technical field
The invention belongs to rice deep process technology field, is related to a kind of method for removing cadmium in rice, and in particular to a kind of
The method of cadmium in the molten combination fermentation technique removing rice of acid.
Background technology
Major producing country of the China as world rice, rice yield account for the 30% of global total output, according to incomplete system
Meter, the population of China more than 60% is using rice as staple food.With the fast development of modern industry, heavy metal cadmium passes through Polluted Soil
Earth invades rice, the pollution problem getting worse of cadmium in rice, it has also become one of great food-safety problem that the whole world faces.
It is thunderous（2010）Investigation and analysis discovery is carried out to the rice of Hunan Province contaminated area and in the market, there are cadmium for 36% rice sample
Exceeded situation；2010 it has been reported that, the arable land of China 1/5 is subject to heavy metal cadmium, is related to 11 and saves 25 areas, main
Will be based on the area on the south the Changjiang river such as Hunan, Jiangxi.The food that heavy metal cadmium is set to priority research by the World Health Organization is dirty
Thing is contaminated, rice has cadmium stronger accumulation ability, and heavy metal cadmium can be accumulated in human body by food chain, and long-term accumulation is made
Certain damage is caused to liver kidney, reproductive system, knot hoof tissue etc. with meeting, or even produces " three-induced effect ", it has been investigated how
Heavy metal cadmium in removing rice urgently needs to solve.
At present, it is a lot of to be related to administer the report of heavy metal cadmium and technological achievement in rice, but is concentrated mainly on big
In terms of the processing technology of rice, such as immersion, boiling, husking, husk rice, the extraction of rice starch and the extraction of rice protein.Due to
Heavy metal cadmium is concentrated mainly in rice endosperm, the removal effect unobvious of husking and husk rice method to cadmium；Liu Jing（2013）
Found during rice in steep, soaking temperature is higher, and the leaching content of cadmium is more, but rice soaks 30h at 30 DEG C, and cadmium takes off
Except rate only has 33.71%, illustrate that removing of the action of soaking of water to cadmium in rice has certain effect, but effect unobvious.Some
Scholar proves to heat the cadmium content that can be reduced to a certain extent in rice by testing, but the removal efficiency of cadmium is relatively low；Field
Sun（2013）Extract rice starch product using alkali process, under optimum process condition, the removal efficiency of cadmium up to 84.77%,
But starch product made from this method differs larger with ative starch quality, and starch extraction rate is relatively low.Chinese Application No.
201510013525.3 document discloses a kind of method for removing heavy metal cadmium in rice using lactobacillus-fermented is compounded, the party
The de- cadmium effect of method is preferable, but needs to further improve.Many research reports in recent years, organic acid can be with heavy metal cadmium
Soluble complexes are combined to form to increase the mobility of cadmium.
The content of the invention
The defects of it is an object of the invention to overcome the prior art, there is provided a kind of cost is low, workable, efficient
The method of heavy metal cadmium, certain technical support is provided for the safety conversion of " cadmium rice " in the molten combination fermentation removing rice of acid,
The Utilizing question of the energy exceeded rice of rapid recovery cadmium.
To achieve the above object, the technical solution adopted in the present invention is：Weight in a kind of molten combination fermentation removing rice of acid
The method of cadmium metal, this method step are as follows：
A. crush：Cadmium content is exceeded（That is cadmium content ＞ 0.2mg/kg）Rice crush, the granularity after crushing is 40-80
Mesh；
B. acid dissolves cadmium：Add the ratio for the acid solution that 6-10mL mass concentrations are 1%-50% in 1g rice meals, take rice meal
With acid solution, mix, stirring, makes rice meal fully be mixed with acid solution, and constant temperature oscillation handles 12-24h at 35-50 DEG C, makes
Cadmium in rice meal is cut down by the molten effect of acid, and frequency of oscillation during oscillation treatment is 160-180 beats/min；
Above-mentioned acid solution is to take the one or more in food grade lactic acid, citric acid, tartaric acid, malic acid and glacial acetic acid to mix
Conjunction is formulated.
C. centrifugation removes acid solution：Acid is dissolved to the rice meal slurries after cadmium to centrifuge（Centrifugal rotational speed is 3000-4500r/min,
Time is 5-10min）To remove acid solution, the rice meal after acid solution is obtained, rice meal is cleaned into 2-4 with deionized water
Secondary, the dosage of deionized water is 3-4 times of the rice meal weight obtained after acid solution during cleaning every time；Carried out every time after cleaning
Centrifugal dehydration（Centrifugal rotational speed is 3000-4500r/min, time 5-10min）, add in the rice meal after last centrifugal dehydration
Enter the deionized water of 3-5 times of rice meal weight, mix, obtain rice meal slurries；
D. cadmium is removed in fermentation：By lactobacillus plantarum bacteria suspension and Pediococcus pentosaceus bacteria suspension by volume 2:1 mixing, is then pressed
The inoculum concentration of 3%-5% is linked into the rice meal slurries that step c is obtained, and is left to ferment 20-28h in 37-40 DEG C of constant temperature, is made rice
Cadmium content in powder further reduces；
The production method of above-mentioned lactobacillus plantarum bacteria suspension and Pediococcus pentosaceus bacteria suspension is existing conventional techniques, specifically
For：The slant strains of lactobacillus plantarum and Pediococcus pentosaceus are cultivated into 24-48h at 37 DEG C respectively, are distinguished again after actication of culture
It is seeded in MRS broth bouillons, the constant temperature incubation 24-48h at 37 DEG C.Gained lactobacillus plantarum bacteria suspension and Pediococcus pentosaceus
The bacteria containing amount of bacteria suspension is all 107-108cfu/mL.The lactobacillus plantarum used and Pediococcus pentosaceus is existing conventional bacterial classifications, such as
Lactobacillus plantarum CICC20038, CICC20039, CICC20765 etc.；Pediococcus pentosaceus CICC22739, CICC21862,
CICC22227 etc..
E. centrifugation filters out zymotic fluid：Above-mentioned fermentation is dropped to the fermentation rice-flour noodles slurries after cadmium to centrifuge（Centrifugal rotational speed is 3000-
4500r/min, time 5-10min）To remove zymotic fluid, the rice meal obtained after removal zymotic fluid is cleaned with deionized water
2-4 times, centrifugal dehydration is carried out after cleaning every time（Centrifugal rotational speed is 3000-4500r/min, time 5-10min）, by last water
The rice meal washed after centrifugation is 11-13% in 45-50 DEG C of heated-air drying to water content；Wherein, the use of deionized water when cleaning every time
Measure to remove 3-5 times of the rice meal weight obtained after zymotic fluid.
In order to further improve the removal efficiency of cadmium in rice, the present invention can be combined to form based on organic acid with heavy metal can
This characteristic of solubleness complex, carries out dipping pretreatment to rice first with organic acid, is tentatively dropped by washing rice and centrifugal dehydration
Heavy metal cadmium content in low rice, then to the processing of rice lactobacillus-fermented for composite, further make the stripping quantity of cadmium increase.
The present invention is combined with fermentation technique by the way that organic acid acid is molten, to heavy metal cadmium of the cadmium content in the rice of more than 0.2mg/kg into
Row removing, the removal efficiency of cadmium is up to more than 90%.Rice meal after de- cadmium is safe, can be used as the grain raw material of feed industry.This
Invent that workable, cost is low, de- cadmium effect is good, can the exceeded rice of rapid recovery cadmium Utilizing question, be " cadmium rice "
Safety conversion provides certain technical support.
Embodiment
The invention will be further described with reference to embodiments.
The preparation of bacteria suspension：The slant strains of lactobacillus plantarum and Pediococcus pentosaceus are cultivated into 24- at 37 DEG C respectively
48h, is seeded in MRS broth bouillons after actication of culture, constant temperature incubation 24-48h, respectively obtains containing bacterium at 37 DEG C respectively again
Measure as 107-108The lactobacillus plantarum bacteria suspension and Pediococcus pentosaceus bacteria suspension of cfu/mL.
Embodiment 1
The rice that cadmium content is 0.6479mg/kg be crushed into 80 mesh sieves；10g rice meals are taken to be added to 60mL mass concentrations
For in 8% glacial acetic acid solution, constant temperature oscillation handles 24h at 35 DEG C after fully mixing（Frequency of oscillation is 160 beats/min）；From
The heart（3500r/min, 8min）Acid solution is removed, the rice meal for going after acid solution to obtain is cleaned 3 times with deionized water（Every time go from
The dosage of sub- water is 4 times of rice meal weight）, every time centrifugal dehydration is carried out after cleaning（3500r/min, 8min）, then at last
The deionized water of 4 times of rice meal weight is added in rice meal after centrifugal dehydration, rice meal slurries are obtained after mixing；By plant breast bar
Bacterium bacteria suspension（Bacterium numbering is CICC20039）With Pediococcus pentosaceus bacteria suspension（Bacterium numbering is CICC22227）By volume
2:1 mixing（The bacteria containing amount of two kinds of bacterium bacteria suspensions is all 107cfu/mL）, then the rice meal slurries are linked into by 3% inoculum concentration
In, 24h is left to ferment in 40 DEG C of constant temperature, obtains fermentation rice-flour noodles slurries；Fermentation rice-flour noodles slurries are centrifuged（3500r/min, 8min）With
Zymotic fluid is filtered out, the rice meal obtained after zymotic fluid is removed and is cleaned 3 times with deionized water（The dosage of each deionized water is rice
4 times of powder weight）, every time centrifugal dehydration is carried out after cleaning（3500r/min, 8min）, by the rice meal after last centrifugal dehydration
In 50 DEG C of heated-air dryings to constant weight（Moisture is 11.9%）, obtain the rice meal after de- cadmium.After testing, cadmium in rice meal
Residual quantity is 0.05851mg/kg, and less than national limit standard 0.2mg/kg, and the removal efficiency of cadmium illustrates this method up to 90.97%
The heavy metal cadmium in rice can effectively be removed.
Control 1：Rice meal is connect without peracid solutions immersion treatment using the bacteria suspension of lactobacillus plantarum and Pediococcus pentosaceus
Kind carries out fermentation process, and other conditions are with embodiment 1, and rice meal is under the immersion of deionized water and fermentation, cadmium in rice
Removal efficiency there was only 83.73%.
Control 2：Rice meal only carries out acid solution immersion treatment, without lactobacillus plantarum and the bacteria suspension of Pediococcus pentosaceus
Inoculation fermentation, other conditions are with embodiment 1, and rice meal is under the sour molten effect of glacial acetic acid, and the removal efficiency of cadmium only has in rice
82.53%。
Embodiment 2
The rice that cadmium content is 0.4745mg/kg be crushed into 60 mesh sieves；10g rice meals are taken to be added to 70mL mass concentrations
For in 10% citric acid solution, constant temperature oscillation handles 20h at 40 DEG C after fully mixing（Frequency of oscillation is 170 beats/min）；
Centrifugation（4500r/min, 5min）Acid solution is removed, the rice meal for going after acid solution to obtain is cleaned 2 times with deionized water（Every time go from
The dosage of sub- water is 3 times of rice meal weight）, every time centrifugal dehydration is carried out after cleaning（4500r/min, 5min）, then at last
The deionized water of 3 times of rice meal weight is added in rice meal after centrifugal dehydration, rice meal slurries are obtained after mixing；By plant breast bar
Bacterium bacteria suspension（Bacterium numbering is CICC20038）With Pediococcus pentosaceus bacteria suspension（Bacterium numbering is CICC22739）By volume
2:1 mixing（The bacteria containing amount of two kinds of bacterium bacteria suspensions is all 108cfu/mL）, then the rice meal slurries are linked into by 4% inoculum concentration
In, 20h is left to ferment in 40 DEG C of constant temperature, obtains fermentation rice-flour noodles slurries；Fermentation rice-flour noodles slurries are centrifuged（4500r/min, 5min）With
Zymotic fluid is filtered out, the rice meal obtained after zymotic fluid is removed and is cleaned 2 times with deionized water（The dosage of each deionized water is rice
5 times of powder weight）, every time centrifugal dehydration is carried out after cleaning（4500r/min, 5min）, by the rice meal after last cleaning centrifugation
In 45 DEG C of heated-air dryings to constant weight（Moisture is 12.5%）, obtain the rice meal after de- cadmium.After testing, cadmium in rice meal
Residual quantity is 0.01794mg/kg, and less than national limit standard 0.2mg/kg, and the removal efficiency of cadmium illustrates this method up to 96.22%
The heavy metal cadmium in rice can effectively be removed.
Embodiment 3
The rice that cadmium content is 0.6479mg/kg be crushed into 80 mesh sieves；10g rice meals are taken to be added to 100mL mass dense
Spend in the food grade lactic acid solution for 50%, constant temperature oscillation handles 12h at 50 DEG C after fully mixing（Frequency of oscillation for 180 times/
Minute）；Centrifugation（3000r/min, 10min）Acid solution is removed, the rice meal for going after acid solution to obtain is cleaned 4 times with deionized water
（The dosage of each deionized water is 4 times of rice meal weight）, every time centrifugal dehydration is carried out after cleaning（3000r/min,
10min）, the deionized water of 5 times of rice meal weight is added in the rice meal after last centrifugal dehydration, rice meal is obtained after mixing
Slurries；By lactobacillus plantarum bacteria suspension（Bacterium numbering is CICC20765）With Pediococcus pentosaceus bacteria suspension（Bacterium numbering is
CICC21862）By volume 2:1 mixing（The bacteria containing amount of two kinds of bacterium bacteria suspensions is all 107cfu/mL）, then by 5% inoculum concentration
It is linked into the rice meal slurries, is left to ferment 28h in 37 DEG C of constant temperature, obtains fermentation rice-flour noodles slurries；Fermentation rice-flour noodles slurries are centrifuged
（3000r/min, 10min）Zymotic fluid is filtered out, the rice meal obtained after removal zymotic fluid is cleaned 4 times with deionized water（Every time
The dosage of deionized water is 3 times of rice meal weight）, every time centrifugal dehydration is carried out after cleaning（3000r/min, 10min）, will most
The rice meal after centrifugal dehydration is in 48 DEG C of heated-air dryings to constant weight afterwards（Moisture is 11.5%）, obtain the rice meal after de- cadmium.
After testing, the residual quantity of cadmium is 0.01289mg/kg in rice meal, less than national limit standard 0.2mg/kg, and the removal efficiency of cadmium
Up to 98.01%, illustrate that this method can effectively remove the heavy metal cadmium in rice.
Embodiment 4
The rice that cadmium content is 0.6479mg/kg be crushed into 80 mesh sieves；10g rice meals are taken to be added to 80mL mixed acid molten
Liquid（Malic acid by the food grade lactic acid solution of 38% mass concentration, the tartaric acid solution of 2% mass concentration and 2% mass concentration is molten
Liquid by volume 2:1：1 mixing）In, constant temperature oscillation handles 18h at 45 DEG C after fully mixing（Frequency of oscillation is 175 beats/min
Clock）；Centrifugation（4000r/min, 7min）Acid solution is removed, the rice meal for going after acid solution to obtain is cleaned 3 times with deionized water（Every time
The dosage of deionized water is 3 times of rice meal weight）, every time centrifugal dehydration is carried out after cleaning（4000r/min, 7min）, then at
The deionized water of 4 times of rice meal weight is added in rice meal after last centrifugal dehydration, rice meal slurries are obtained after mixing；By plant
Lactobacillus bacteria suspension（Bacterium numbering is CICC20039）With Pediococcus pentosaceus bacteria suspension（Bacterium numbering is CICC22227）Press body
Product ratio 2:1 mixing（The bacteria suspension bacteria containing amount of two kinds of bacterium is all 108cfu/mL）, then the rice meal is linked into by 3% inoculum concentration
In slurries, 24h is left to ferment in 38 DEG C of constant temperature, obtains fermentation rice-flour noodles slurries；Fermentation rice-flour noodles slurries are centrifuged（4000r/min,
7min）Zymotic fluid is filtered out, the rice meal obtained after zymotic fluid is removed and is cleaned 3 times with deionized water（The dosage of each deionized water
For 4 times of rice meal weight）, every time centrifugal dehydration is carried out after cleaning（4000r/min, 7min）, after last centrifugal dehydration
Rice meal is in 46 DEG C of heated-air dryings to constant weight（Moisture is 12.1%）, obtain the rice meal after de- cadmium.After testing, rice meal
The residual quantity of middle cadmium is 0.01419mg/kg, and less than national limit standard 0.2mg/kg, and the removal efficiency of cadmium is up to 97.81%, explanation
This method can effectively remove the heavy metal cadmium in rice.
Claims (8)
1. a kind of method of cadmium in molten combination fermentation removing rice of acid, it is characterised in that this method step is as follows：
A. crush：The exceeded rice of cadmium content is crushed；
B. acid dissolves cadmium：In 1g rice meals add 6-10mL mass concentrations be 1%-50% acid solution ratio, by rice meal with
Acid solution fully mixes, and constant temperature oscillation handles 12-24h at 35-50 DEG C, obtains rice meal slurries；Wherein, acid solution is feeding product
One or more in level lactic acid, citric acid, tartaric acid, malic acid or glacial acetic acid are formulated；
C. centrifugation removes acid solution：Acid is dissolved to the rice meal slurries after cadmium to centrifuge, the rice meal after acid solution must be removed, by rice
Powder is cleaned 2-4 times with deionized water, and the dosage of deionized water is 3-4 times of rice meal weight during cleaning every time, after cleaning every time
Centrifugal dehydration is carried out, the deionized water of 3-5 times of rice meal weight is added in the rice meal after last centrifugal dehydration, mixes, obtains
Rice meal slurries；
D. cadmium is removed in fermentation：By lactobacillus plantarum bacteria suspension and Pediococcus pentosaceus bacteria suspension by volume 2:1 mixing, then by 3%-
5% inoculum concentration is linked into the rice meal slurries that step c is obtained, and is left to ferment 20-28h in 37-40 DEG C of constant temperature, is obtained fermentation rice
Slurry liquid；Wherein, the bacteria containing amount of lactobacillus plantarum bacteria suspension and Pediococcus pentosaceus bacteria suspension is all 107-108cfu/mL；
E. centrifugation filters out zymotic fluid：Above-mentioned fermentation rice-flour noodles slurries are centrifuged, then are cleaned 2-4 times with deionized water, every time after cleaning
Centrifugal dehydration is carried out, the rice meal after last washing is centrifuged is 11-13% in 45-50 DEG C of heated-air drying to water content；Wherein,
The dosage of deionized water is 3-5 times of rice meal weight during cleaning every time.
2. the method for cadmium in the molten combination fermentation removing rice of a kind of acid as claimed in claim 1, it is characterised in that the step
The exceeded rice of cadmium content refers to the cadmium content ＞ 0.2mg/kg in rice in a.
3. the method for cadmium in the molten combination fermentation removing rice of a kind of acid as claimed in claim 1, it is characterised in that the step
Rice granularity after being crushed in a is 40-80 mesh.
4. the method for cadmium in the molten combination fermentation removing rice of a kind of acid as claimed in claim 1, it is characterised in that the step
Frequency in b during oscillation treatment is 160-180 beats/min.
5. the method for cadmium in the molten combination fermentation removing rice of a kind of acid as claimed in claim 1, it is characterised in that the step
The rotating speed dissolved acid in c when the rice meal slurries after cadmium centrifuge is 3000-4500r/min, time 5-10min.
6. the method for cadmium in the molten combination fermentation removing rice of a kind of acid as claimed in claim 1, it is characterised in that the step
Rotating speed after being cleaned every time in c during centrifugal dehydration is 3000-4500r/min, time 5-10min.
7. the method for cadmium in the molten combination fermentation removing rice of a kind of acid as claimed in claim 1, it is characterised in that the step
Rotating speed when fermentation rice-flour noodles slurries centrifuge in e is 3000-4500r/min, time 5-10min.
8. the method for cadmium in the molten combination fermentation removing rice of a kind of acid as claimed in claim 1, it is characterised in that the step
Rotating speed after being cleaned every time in e during centrifugal dehydration is 3000-4500r/min, time 5-10min.
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| CN105231106A (en) * | 2015-11-23 | 2016-01-13 | 南京财经大学 | Method for removing cadmium in coarse rice powder or refined rice powder |
| CN105455013A (en) * | 2015-12-11 | 2016-04-06 | 中国农业科学院农产品加工研究所 | Production process for cadmium-removed reproduced rice |
| WO2019152427A1 (en) * | 2018-01-31 | 2019-08-08 | Shaklee Corporation | Removing heavy metals from rice protein material |
| CN108467862A (en) * | 2018-03-30 | 2018-08-31 | 武汉轻工大学 | A kind of preparation method that microorganism takes off cadmium adsorbent and its application in powder food takes off cadmium |
| CN110226705A (en) * | 2019-07-24 | 2019-09-13 | 湖南粮食集团有限责任公司 | A method of strengthening cadmium in spontaneous fermentation abatement rice containing cadmium |
| CN111602764A (en) * | 2020-06-01 | 2020-09-01 | 方家铺子（莆田）绿色食品有限公司 | Rice cadmium-removing processing equipment |
| CN115581277A (en) * | 2022-07-01 | 2023-01-10 | 江西省德兴市百勤异Vc钠有限公司 | Cadmium reduction treatment method for cadmium-polluted rice |
| CN115413750B (en) * | 2022-08-29 | 2023-11-03 | 日日佳(湖南)粮油食品股份有限公司 | Rice processing cadmium removing equipment and cadmium removing method |
| CN116570006A (en) * | 2023-05-18 | 2023-08-11 | 江南大学 | Process for removing cadmium in rice by low-intensity ultrasonic-assisted artificial fermentation |
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