CN102199582B - Method for extraction and preservation of helicase - Google Patents
Method for extraction and preservation of helicase Download PDFInfo
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- CN102199582B CN102199582B CN 201010157866 CN201010157866A CN102199582B CN 102199582 B CN102199582 B CN 102199582B CN 201010157866 CN201010157866 CN 201010157866 CN 201010157866 A CN201010157866 A CN 201010157866A CN 102199582 B CN102199582 B CN 102199582B
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Abstract
The invention discloses a method for extraction and preservation of helicase. According to the method, a snail is selected and treated, and the digestive juice of the snail is extracted, filtered and then centrifugalized for refinement, and then subjected to a vacuum freeze-drying treatment so as to be packed and stored, with 9 kinds of enzyme valuable in application effectively extracted from the snail digestive gland. Adoption of the method provided in the invention can realize the volume production of helicase which can be preserved for 10 years at a normal temperature, while helicase products still maintain good activity. Therefore, the method has good economic benefit.
Description
Technical field
The present invention relates to a kind of method of effectively preserving under helicase and the normal temperature of from the snail digestive gland, extracting.
Background technology
But snail is a kind of animal of artificial breeding, and is rich in various enzymes in the snail Digestive system, always has kind more than 20; Helicase is a kind of mixed enzyme, it is the product of snail comprehensive development and utilization, it can be used as the low toolenzyme that waits eukaryotic microorganisms and plant cell wall of dissolving, be widely used in the research of cytobiology and genetic engineering, have following nine kinds of having of using value in the helicase: cellulase, polygalacturonase, A-amylase, 4-B-D seminose restriction endonuclease, B-D glucose hydrolysis enzyme, A-seminase, mannitol oxydase, aminotransferase and Pseudocholinesterase.And at present the extractive technique of helicase is low, and the high so that helicase of preservation condition holds at high price.
Summary of the invention
The object of the present invention is to provide that a kind of technique is simple, strong operability, extraction and the store method of the high and helicase that can effectively preserve of purification rate.
Technical scheme provided by the invention is as follows:
(1) selects the above fresh and alive snail of 15 grams, African Snail (formal name used at school: Achatina fulica) You Jia;
(2) be positioned over the bottom and be with in the foraminate plastic box and rest, every case utilizes space about 30% for good;
(3) only starve more than 5 days, every day, each spent flushing with clean water once with 20 ℃ ~ 30 ℃ sooner or later, should stir plastic box during flushing, utilize current to rinse out to be attached to ight soil and foreign material on the snail shell, should carefully check during flushing, remove downright bad snail, in case hungry snail eats dead snail corpse, guarantee the helicase quality;
(4) evenly firmly crack snail shell with hammer behind the clean snail with on the worktable, carefully digestive gland is peeled off, under 15 ℃ the Working environment Digestive system is being extruded in clean plastic containers, filter by 450 order filtering nets at last and concentrate;
(5) Digestive system after the extraction separates more than 10 minutes at the whizzer by 8000 ~ 10000 rev/mins of rotating speeds under the Working environment below 10 ℃, and the dregs that sink to the bottom are separated;
(6) the supernatant liquid body hypothermia freeze-drying after will separating, it is good that container adopts plastics, vacuum-freeze-dry 18 hours, the helicase water content is below 5%, and the helicase of freeze-drying is xln, is light coffee color;
(7) helicase after the freeze-drying carries out vacuum packaging at dry weather with the high-pressure plastic bag, several finished products are packed with plastic bag sealing again, put into afterwards carton, helicase product after the packing should be positioned over the room in clean cool place, product overhead reaches more than the wall 40CM, must not stack with the peculiar smell article.
From foregoing description as can be known, helicase of the present invention extract and store method simple to operation, used equipment is conventional article, can realize the industrialization volume production, that the helicase of producing has is aseptic, activity is high, the characteristics of long preservative period.
Embodiment
A kind of preferred embodiment of the present invention, select the above fresh and alive African Snail of 15 grams, putting into the foraminate plastic box in bottom rests, the space that utilizes of each plastic box is 30% effect, only starves more than 5 days, and every day is sooner or later respectively with 20 ~ 30 ℃ of flushing with clean water snails, stir plastic box during flushing, utilize current to wash out to be attached to ight soil and foreign material on the snail shell, care should be used to checks during flushing, and the snail of checkmating is removed;
Get and on worktable, evenly firmly crack snail shell with hammer after the 50KG snail is cleaned, under 15 ℃ the Working environment Digestive system is being extruded in clean plastic containers after digestive gland is peeled off, filter by 450 order filtering nets at last and concentrate;
The snail Digestive system collected at 10 ℃, is separated on 8000 ~ 10000 rev/mins the whizzer, 12 minutes operating times, then the supernatant liquid of separator well to be poured out, lower floor's dregs are abandoned, and can get 1000 milliliters of clear liquors;
Isolated supernatant liquid carries out low-temperature freeze drying in plastic containers, vacuum-freeze-dry 18 as a child, helicase water content about 4%, the helicase after the freeze-drying is xln, is light coffee color, can get 145 the gram xln;
Helicase after the freeze-drying carries out vacuum packaging at dry weather with the high-pressure plastic bag, several finished products are packed with plastic bag sealing again, put into afterwards carton, helicase product after the packing should be positioned over the room in clean cool place, product overhead reaches more than the wall 40CM, must not stack with the peculiar smell article.
Above-mentioned only is a specific embodiment of the present invention, but technique of the present invention is not limited to this, and all changes that utilizes this processing method the present invention to be carried out unsubstantiality all should belong to the behavior of invading protection domain of the present invention.
Claims (1)
1. the extraction of a helicase and store method is characterized in that:
(1) selects the above fresh and alive snail of 15 grams;
(2) only starve more than 5 days, every day is sooner or later with 20 ℃～30 ℃ degree flushing with clean water;
(3) break snail shell into pieces behind the clean snail, carefully digestive gland is peeled off, under 15 ℃ Working environment, Digestive system is extruded in container, filter by 450 order filtering nets at last and concentrate;
(4) Digestive system after the extraction separates more than 10 minutes at the whizzer by 8000～10000 rev/mins of rotating speeds under the Working environment below 10 ℃, and the dregs that sink to the bottom are separated;
(5) the supernatant liquid body hypothermia freeze-drying after will separating, container adopts plastics, vacuum-freeze-dry 18 hours, the helicase water content is below 5%, and the helicase of freeze-drying is xln, is light coffee color;
(6) helicase after the freeze-drying carries out vacuum packaging at dry weather with the high-pressure plastic bag, several finished products are packed with plastic bag sealing again, put into afterwards carton, helicase product after the packing should be positioned over the room in clean cool place, product overhead reaches more than the wall 40CM, must not stack with the peculiar smell article.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010157866 CN102199582B (en) | 2010-03-23 | 2010-03-23 | Method for extraction and preservation of helicase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010157866 CN102199582B (en) | 2010-03-23 | 2010-03-23 | Method for extraction and preservation of helicase |
Publications (2)
| Publication Number | Publication Date |
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| CN102199582A CN102199582A (en) | 2011-09-28 |
| CN102199582B true CN102199582B (en) | 2013-04-10 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 201010157866 Active CN102199582B (en) | 2010-03-23 | 2010-03-23 | Method for extraction and preservation of helicase |
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| CN (1) | CN102199582B (en) |
Families Citing this family (13)
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| CN103468663A (en) * | 2013-08-12 | 2013-12-25 | 叶阿彬 | Snail protease extraction method |
| CN104560931B (en) * | 2015-02-16 | 2018-03-06 | 威海瑞佳丽生物科技有限公司 | A kind of extracting method of glusulase |
| CN104719794A (en) * | 2015-04-20 | 2015-06-24 | 威海圣奥罗生物科技有限公司 | Guava fruit slice preparation method |
| CN104726440A (en) * | 2015-04-20 | 2015-06-24 | 威海圣奥罗生物科技有限公司 | Snail enzyme preparation method |
| CN104738460A (en) * | 2015-04-20 | 2015-07-01 | 威海圣奥罗生物科技有限公司 | Preparation method of Chinese yam slice |
| CN104738454A (en) * | 2015-04-20 | 2015-07-01 | 威海圣奥罗生物科技有限公司 | Preparation method of fig slices |
| CN104839579A (en) * | 2015-04-20 | 2015-08-19 | 威海圣奥罗生物科技有限公司 | Preparation method of sliced balsam pear |
| CN104745555A (en) * | 2015-04-20 | 2015-07-01 | 威海圣奥罗生物科技有限公司 | Preparation method of snail protease |
| CN106117428A (en) * | 2016-06-25 | 2016-11-16 | 陈毅忠 | A kind of method utilizing Limax to prepare water-absorbing resin |
| CN106086118A (en) * | 2016-06-25 | 2016-11-09 | 仇颖超 | A kind of method that Limax resource prepares chitin oligosaccharide |
| CN105925644A (en) * | 2016-06-29 | 2016-09-07 | 郭舒洋 | Method for synthesizing arbutin by catalyzing through immobilized biological complex enzyme |
| CN106036984B (en) * | 2016-07-03 | 2017-08-08 | 赖高生 | A kind of method that immobilization glusulase prepares tobacco extract |
| CN106498801B (en) * | 2016-10-11 | 2018-03-16 | 广博集团股份有限公司 | A kind of preparation method of papermaking special environment protection stalk pulping agents |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1099415A (en) * | 1994-07-02 | 1995-03-01 | 张华玲 | Snail bioenzyme and snail biotin |
| CN1216778A (en) * | 1998-10-10 | 1999-05-19 | 复旦大学 | Method for preparing cepaeo enzyme |
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2010
- 2010-03-23 CN CN 201010157866 patent/CN102199582B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1099415A (en) * | 1994-07-02 | 1995-03-01 | 张华玲 | Snail bioenzyme and snail biotin |
| CN1216778A (en) * | 1998-10-10 | 1999-05-19 | 复旦大学 | Method for preparing cepaeo enzyme |
Non-Patent Citations (2)
| Title |
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| 孙祖莉等.蜗牛纤维素酶提取工艺的初步研究.《生态科学》.2002,第21卷(第04期),327-329. * |
| 惠军等.新疆蜗牛酶的研制.《新疆师范大学学报(自然科学版)》.2006,第25卷(第03期),90-92. * |
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| CN102199582A (en) | 2011-09-28 |
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Application publication date: 20110928 Assignee: YESHI BIOLOGICAL TECHNOLOGY CO., LTD. Assignor: Ye Abin Contract record no.: 2014350000046 Denomination of invention: Method for extraction and preservation of helicase Granted publication date: 20130410 License type: Exclusive License Record date: 20140429 |
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