CN107259293A - A kind of method that natural is prepared from clam worm - Google Patents
A kind of method that natural is prepared from clam worm Download PDFInfo
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- CN107259293A CN107259293A CN201710468547.8A CN201710468547A CN107259293A CN 107259293 A CN107259293 A CN 107259293A CN 201710468547 A CN201710468547 A CN 201710468547A CN 107259293 A CN107259293 A CN 107259293A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3472—Compounds of undetermined constitution obtained from animals or plants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
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Abstract
The invention discloses a kind of method that natural is prepared from clam worm, using clam worm as raw material, clam worm is after the dark and damp environment storage processing of seawater, after deionized water rinsing, homogenate, ultrasound, soaked overnight, boil until milkiness is presented in clam worm skeleton, after filtering and impurity removing matter, concentrated frozen is dried, and obtains product.Employ aqueous phase soak extraction, decoct partition method, milipore filter concentration and freeze drying techniques, without using other chemical reagent, operating process is simple, and process stabilizing performance is good, and recovery rate is high.Oxidation resistant product produced by the present invention preserves stable, with good antioxidation activity, can substantially remove the free radicals such as superoxide anion.Therefore, this product all has good research and application prospect in terms of medicine, health care.
Description
Technical field
The invention belongs to a kind of method that natural is prepared from clam worm of field of biological product.
Background technology
Clam worm belongs to Annelida, polychaeta, Errantia, Nereidae on taxology, is commonly called as marine borer, extra large maggot, sea Wu
Centipede, extra large leech.The clam worm species of China has kind about more than 80, and economic species and the kind for cultivation mainly have：Neantes japonica,
Thorniness encloses clam worm, perinereis aibihitensis Grube etc..Like to inhabit husky mud of the area to subtidal zone in the Coastal beach for thering is fresh water to flow into, intertidal zone
In, larva food planktonic organism, adult is using humus as food.Clam worm has very high nutritive value, is obtained in aquaculture extensively
Using.Clam worm（Nereid）English name-Clamworm, belongs to Annelida, polychaeta leaf must the section of worm purpose 1, economic value compared with
It is high.
Clam worm body elongated cylindrical, both sides are symmetrical, rear end is sharp, and tool is many（More than 80~200）Body segment.Head, body can be divided into
Cadre and afterbody.Head is flourishing, is made up of two major parts of prostomium and peristomium.Prostomium is to stretch in the circle three in front of mouth
Angular or conical meat pallette, has 2 pairs of simple circular eyes, and the cephalic tentacle and its front end veutro of 1~2 extension are two big
The feeler of merogenesis.Peristomium is a big ring joint, the mouth that outside of belly tool is grown crosswise, its both sides 3~4 pairs of antennas of tool, the kiss of muscle matter
It can be stretched out by mouth, kiss front end has 1 pair of chitin big jaw, and kiss surface is smooth or has chitin jaw tooth or soft mastoid process；Trunk has perhaps
The similar body segment of many structures, each body segment both sides have the overhanging flat projection of meat, i.e. parapodum.Parapodum is generally foot in bifolium tool
Thorn, there is thorn-like or falciform compound seta outside；Afterbody is last 1 section or several sections of of polypide, also known as anal segment, and tool a pair of anuses palpuses, anuses are opened
Mouth is in anal segment opposing ends.Bristle has poison gland, and thorn has the phenomenon of congestion and swelling pain to skin.
Clam worm is nutritious, is not only the food organisms of fishes and shrimps addiction, can also be used as excellent bait.Coastal resident is also
The clam worm of reproduction gland maturity is regarded as nutrition treasure.After drying, the white such as milk of soup is boiled, taste is extremely delicious, and concentration is big, there is " natural taste
The title of essence ".Fried rear loose delicious and crisp, for delicacies of going with wine.Clam worm no matter at home or outlet, it is all in very great demand.In recent years, clam worm
More than the comparison of research is some active components extracted from it, such as bacteriostatic peptide, clam worm element etc..,
Further, since clam worm has good environmental suitability, some researchers indicate it as ecological key species
Environmental.
Document also reported a set of antioxidant system being had by oneself in clam worm body, it was recently reported that clam worm can be good at preventing lead from
The murder by poisoning of son, with good anti-oxidation function, but article does not report how the antioxidant system is prepared into antioxidant system
The method and relevant report of product.It is an object of the invention to provide a kind of method that natural is prepared from clam worm, with more
Mend the deficiency having been reported.
The content of the invention
It is an object of the invention to provide a kind of method that natural is prepared from clam worm, prepared natural antioxygen
Agent has the effect for removing free radical, available for medicine and the anti-oxidation protection of food, can also be used as food additives and guarantor
Strong product are used to remove free radical in vivo, prevention senility of humanbody etc..
The present invention uses clam worm for unique raw material, and such as Neantes japonica, thorniness enclose clam worm, perinereis aibihitensis Grube, raw material
It is natural marine organism, is the abundant food materials of nutritive value, preparation process need not adds the chemistry examination of other toxic side effects
Agent and auxiliary agent, preparation process environmental protection, without environmental pollution, Product Green, degradable absorption has no side effect safely.
The present invention is clam worm using raw material, and such as Neantes japonica, thorniness enclose clam worm, perinereis aibihitensis Grube, but are not limited to
These three clam worm raw materials, the technology of preparation method of all clam worms in the present invention is identical.
The raw material used in the present invention is fresh and healthy clam worm, by the dark and damp environmental treatment of seawater, is temporarily supported with clean seawater,
With deionized water rinsing, surface impurity and mucus are removed, the preparation of clam worm natural in the present invention is subsequently used for.
Time of the clam worm by the storage processing of seawater dark and damp environment is used in the present invention for 1-7 days, temporarily supported with seawater when
Between be 1-3 days, with deionized water rinsing, after removal surface impurity and mucus, pull out rapidly for clam worm natural
Prepare, can also -20 DEG C of stored frozens it is standby.
Clam worm is cut into chunks shape first in the present invention, then homogenized is carried out with tissue homogenizer, further ultrasound is broken
It is broken, it is broken after add deionized water added into system, be standing and soak for extracting, heating separation, be filtrated to get the first crude extract and
First extracts residue.
Homogenized after clam worm is cut off in the present invention, rotating speed 5000-10000 rpm, 10-15 min are further ultrasonic
Broken 10-20 min, it is 10-100 times of clam worm weight to add the deionized water added into system after crushing, and dwell temperature is
4 DEG C of -45 DEG C of soak extraction times are 8-20 h, and the condition of heating separation is the lower 60-90 min of boiling temperature.
Used in the present invention and add deionized water into the first extraction residue, stand 1-5 hours, plus the amount of deionized water is
5-50 times of clam worm weight, boils 5-30 minutes, filtering, obtains the second crude extract and second and extracts residue.
The first crude extract and the second crude extract are merged in the present invention, 4 DEG C of -10 DEG C of stand at low temperature 5-10 hour are filtered
Not tolerant is removed, filtrate is collected, is concentrated, freezed dried with milipore filter, sealing preserve obtains the clam worm natural anti-oxidation of the present invention
Agent product.
Present invention employs aqueous phase soak extraction, partition method, milipore filter concentration and freeze drying techniques are decocted, without using it
His chemical reagent, operating process is simple, and process stabilizing performance is good, and recovery rate is high.Oxidation resistant product produced by the present invention preserves steady
It is fixed, with good antioxidation activity, the free radicals such as superoxide anion can be substantially removed, therefore, this product is in medicine, health care etc.
Aspect all has good research and application prospect.And with the specific examples below process that the present invention is furture elucidated.
Embodiment
Clam worm is by the dark and damp environment storage of seawater, after seawater is temporarily supported, and weighs fresh 1 part of clam worm, and deionized water rinsing is removed
Surface impurity and blood.Clam worm is cut into section shape, homogenate, ultrasound by scissors.37 DEG C of soaked overnights in 30 parts of deionized waters,
The clam worm of soaked overnight boils 30 min, to be cooled to add 15 parts of deionized water to continue to boil until white is presented in clam worm skeleton later
Shape, by mixture double gauze filter 23, goes the removal of impurity completely, by filtrate stand at low temperature 1-5 hours of collection, after concentration
Freeze-drying, products obtained therefrom is placed in 4 DEG C of refrigerator preservations.
The manufacture method of the present invention is further provided exemplified by following specific embodiment.
Embodiment 1
Neantes japonica is placed in the dark and damp environment of seawater and handled 1 day, seawater is temporarily supported 1 day.Take 1 part of clam worm, deionized water rinsing,
Remove surface impurity and blood.5000 rpm homogenate 15 min, the min of ultrasonication 10 after clam worm individual is cut.30 parts
Deionized water is cleaned after soaked overnight, decocts 1h, after cooling plus 15 parts of deionized water continues to boil 20 min, will decoction liquor twice
Mixing, 4 DEG C of 5 h of standing, are freeze-dried after concentration after cooling, that is, obtain clam worm oxidation resistant product, and product is viscous brown shape,
Yield is 13.63%.
Embodiment 2
Thorniness is enclosed into clam worm and is placed in processing 7 days in the dark and damp environment of seawater, seawater is temporarily supported 3 days.Take 1 part of clam worm, deionized water rinsing,
Remove surface impurity and blood.10000 rpm homogenate 25 min, the min of ultrasonication 20 after clam worm individual is cut.30 parts
Deionized water is cleaned after soaked overnight, decocts 1h, after cooling plus 15 parts of deionized water continues to boil 20 min, will decoction liquor twice
Mixing, 4 DEG C of 5 h of standing, are freeze-dried after concentration after cooling, that is, obtain clam worm oxidation resistant product, and product is that rufous is sticky
Shape, yield is 10.80%.
Embodiment 3
Perinereis aibihitensis Grube is placed in the dark and damp environment of seawater and handled 3 days, seawater is temporarily supported 2 days.Take 1 part of clam worm, deionized water rinsing,
Remove surface impurity and blood.5000 rpm homogenate 15 min, the min of ultrasonication 10 after clam worm individual is cut.30 parts
Deionized water is cleaned after soaked overnight, decocts 1h, after cooling plus 15 parts of deionized water continues to boil 20 min, will decoction liquor twice
Mixing, 4 DEG C of 5 h of standing, are freeze-dried after concentration after cooling, that is, obtain clam worm oxidation resistant product, and product is that rufous is sticky
Shape, yield is 11.68%.
Embodiment 4
Neantes japonica, thorniness are enclosed into clam worm, perinereis aibihitensis Grube and are respectively placed in processing 3 days in the dark and damp environment of seawater, seawater temporarily supports 2
My god.1 part of clam worm of mixing is taken, deionized water rinsing removes surface impurity and blood.10000 rpm after clam worm individual is cut
It is homogenized 20 min, the min of ultrasonication 20.30 parts of deionized waters are cleaned after soaked overnight, decoct 1h, and deionized water is added after cooling
15 parts are continued to boil 20 min, decoction liquor will be mixed twice, 4 DEG C of 5 h of standing, are freeze-dried after concentration, that is, obtain sand after cooling
Silkworm oxidation resistant product, product is that reddish brown is thick, and yield is 19.66%.
Anti-oxidant research
Clearance rate of the product to superoxide anion and hydroxyl radical free radical is probed into the present invention, its results icon is as follows：
Fig. 1, removing of the extracts from Perinereis aibuhitensis to superoxide anion.Fig. 2, removing of the extracts from Perinereis aibuhitensis to hydroxyl radical free radical.
By the results show, with the increase of material concentration, its clearance rate to superoxide anion and hydroxyl radical free radical
All it is increased, 60% has been reached in 40 mg/ml, illustrates that it has obvious antioxidation, therefore in medicine, health care etc.
Aspect all has good research and application prospect.
Claims (4)
1. the invention discloses a kind of method that natural is prepared from clam worm, it is characterized in that using clam worm as raw material, it is husky
Silkworm is after the dark and damp environment storage processing of seawater, deionized water rinsing, homogenate, ultrasound, after soaked overnight, boils until clam worm bone
Frame is presented after milkiness, filtering and impurity removing matter, and concentrated frozen is dried, and obtains product.
2. the manufacture method of the natural according to claim 1, it is characterized in that using clam worm as raw material, such as Japan
Thorn clam worm, thorniness enclose clam worm, perinereis aibihitensis Grube etc., but be not limited to these three clam worm raw materials, and all clam worms are in the present invention
Technology of preparation method is identical.
3. the manufacture method of the natural according to claim 1, it is characterized in that clam worm passes through dark and damp environmental treatment
1-7 days, using aqueous phase soak extraction, decoct separation method and obtain through refining standby polyphenoils.
4. the manufacture method of the natural according to claim 1, it is characterized in that 30 parts of deionized waters clean immersion
After overnight, 1h is decocted, adds 15 parts of deionized water to continue to boil 20 min after cooling.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710468547.8A CN107259293A (en) | 2017-06-20 | 2017-06-20 | A kind of method that natural is prepared from clam worm |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710468547.8A CN107259293A (en) | 2017-06-20 | 2017-06-20 | A kind of method that natural is prepared from clam worm |
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| Publication Number | Publication Date |
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| CN107259293A true CN107259293A (en) | 2017-10-20 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710468547.8A Pending CN107259293A (en) | 2017-06-20 | 2017-06-20 | A kind of method that natural is prepared from clam worm |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111265552A (en) * | 2018-12-04 | 2020-06-12 | 中国海洋大学 | Preparation method of clam worm antithrombotic active substance |
| CN111647094A (en) * | 2020-06-11 | 2020-09-11 | 福建省水产研究所（福建水产病害防治中心） | Method for extracting nereis polysaccharide with auxiliary protection effect on chemical liver injury |
| CN114736848A (en) * | 2022-06-13 | 2022-07-12 | 山东新希望六和集团有限公司 | Application of long-beard nereis polypeptide in chicken egg vacuole granular cell proliferation and progesterone secretion |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1521188A (en) * | 2003-01-28 | 2004-08-18 | 中国科学院动物研究所 | Protein extracted from perinereis aibuhitensis, its preparation method and use |
| CN105029635A (en) * | 2015-06-24 | 2015-11-11 | 广东海洋大学 | Clamworm ultrahigh pressure extraction method and clamworm extractive prepared according to method |
| CN106722934A (en) * | 2016-11-25 | 2017-05-31 | 济南睿呋康生物科技有限公司 | A kind of anti-ageing health food for containing peptide extract containing earthworm, clam worm |
-
2017
- 2017-06-20 CN CN201710468547.8A patent/CN107259293A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1521188A (en) * | 2003-01-28 | 2004-08-18 | 中国科学院动物研究所 | Protein extracted from perinereis aibuhitensis, its preparation method and use |
| CN105029635A (en) * | 2015-06-24 | 2015-11-11 | 广东海洋大学 | Clamworm ultrahigh pressure extraction method and clamworm extractive prepared according to method |
| CN106722934A (en) * | 2016-11-25 | 2017-05-31 | 济南睿呋康生物科技有限公司 | A kind of anti-ageing health food for containing peptide extract containing earthworm, clam worm |
Non-Patent Citations (1)
| Title |
|---|
| 黄琳等: "沙蚕提取物的抗氧化活性研究", 《中国海洋药物》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111265552A (en) * | 2018-12-04 | 2020-06-12 | 中国海洋大学 | Preparation method of clam worm antithrombotic active substance |
| CN111647094A (en) * | 2020-06-11 | 2020-09-11 | 福建省水产研究所（福建水产病害防治中心） | Method for extracting nereis polysaccharide with auxiliary protection effect on chemical liver injury |
| CN111647094B (en) * | 2020-06-11 | 2021-11-23 | 福建省水产研究所（福建水产病害防治中心） | Method for extracting nereis polysaccharide with auxiliary protection effect on chemical liver injury |
| CN114736848A (en) * | 2022-06-13 | 2022-07-12 | 山东新希望六和集团有限公司 | Application of long-beard nereis polypeptide in chicken egg vacuole granular cell proliferation and progesterone secretion |
| CN114736848B (en) * | 2022-06-13 | 2022-10-04 | 山东新希望六和集团有限公司 | Application of long-beard nereis polypeptide in chicken egg vacuole granular cell proliferation and progesterone secretion |
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Application publication date: 20171020 |