CN104829734B - A kind of method of utilization dried thin mushroom production pigment, albumen, polysaccharide and dietary fiber - Google Patents
A kind of method of utilization dried thin mushroom production pigment, albumen, polysaccharide and dietary fiber Download PDFInfo
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Abstract
The invention discloses a kind of comprehensive utilization process of dried thin mushroom, including the method for preparing mushroom pigment, protein from lentinus edodes, lentinan and mushroom dietary fiber, beneficial effects of the present invention are as follows：The use that the technique passes through the physical methods such as low temperature, high temperature, membrane filtration, realize the comprehensive utilization of mushroom low cost, the bioactivity of each extract is maintained to greatest extent, in turn ensure that its security, the pigment that is produced by the method, albumen, polysaccharide, dietary fiber can be directly appended in food, not only nutrition and health care but also safety.
Description
Technical field
The present invention relates to a kind of deep-processing and colligating-using technique of dried thin mushroom, more particularly to one kind is same from dried thin mushroom
When extract mushroom pigment, protein from lentinus edodes, lentinan and mushroom dietary fiber method.
Background technology
Mushroom is the fructification of Mycophyta door fungi mushroom, belongs to Basidiomycetes Agaricaceae, is famous edible in the world
One of bacterium and medicinal fungus.Due to nutritious, fragrance oozes spleen, and delicious flavour have " king in mushroom ", " mushroom queen ", " vegetables
Hat " laudatory title.
Mushroom is with high protein, low fat, several amino acids and multivitamin mushroom food.Dried thin mushroom eats portion
Point account for 72%, per 100g aqueous 13g in edible part, fat 1.8g, protein 17g, carbohydrate 54g, crude fibre 7.8g,
Ash content 4.9g.
Substantial amounts of scientific research both at home and abroad at present has been proven that the multiple biological activities of lentinan, such as lentinan
The foundation of mouse Th1 type immune responses can be excited；Can recover and protect the splenic function of rat bearing S180, enhancing body to exempt from
Epidemic disease function；There is antitumor, oxidation resistant effect to rat；Influenza virus can be suppressed to replicate in vivo；Being capable of anti-listeria
Infection, anti-malarial；There is protective effect to radiation injury；Being capable of fatigue-relieving；Also there is protective effect to optic nerve injury.Correlation is ground
Study carefully and also show：There is active cell to be immunized, various humoral indications are adjusted for lentinan, induction alpha-interferon generation, regulation
Immune response reacts, and induction leucocyte causes tumor locus blood vessel dilatation, bleeding, necrosis to tumor-infiltrated, prevents virus
And the combination of host cell, raising SOD activity, suppress MDA generations, and lipid peroxidation inhibition reduces cholesterol, adjusts glycometabolism, changes
Kind sugar tolerance, expansion intestines and stomach produce satiety and mitigate appetite, reduce the effect such as blood sugar.
In the prior art, some report with separating at home and abroad the extraction of Lentinan and albumen, but text
The preparation for offering lentinan is required to settle polysaccharide from weak solution using ethanol, and the preparation of protein from lentinus edodes is then needed
To be both needed to use ethanol in these techniques using the method for alkali carries alcohol precipitation, on the one hand because ethanol consumption is big, high cost；Separately
On the one hand because ethanol is flammable, there is potential safety hazard, the industrialization production of lentinan is constrained to a certain extent.
If a kind of safety, the production technology of low cost can be studied, while lentinan is extracted, by its in mushroom
His nutritional ingredient also extraction and application in the lump, that will greatly facilitate the lifting of mushroom process deeply industry technical merit, realize
The conversion increment of mushroom.
The content of the invention
It is an object of the invention to provide a kind of comprehensive utilization process of dried thin mushroom, can extract simultaneously simultaneously in the process
Mushroom pigment, protein from lentinus edodes, lentinan and mushroom dietary fiber are obtained, realization makes full use of resource, technique to greatest extent
Energy consumption is low and safe, meets the resource-conserving Development Strategy required by current China, while also comply with extension agricultural product producing
Industry chain, drives the development thought that agricultural product industrialization level and agricultural product comprehensive benefit are improved comprehensively.
Technical scheme is comprised the following steps：
(1) dried thin mushroom is crushed：Dried thin mushroom is pulverized and sieved, it is desirable to which the grinding particle size of material is between 20~60 mesh；
(2) cold water extraction：Material after crushing is pressed into solid-liquid mass ratio 1:(20~25) add water carries out dispensing, stirs
Afterwards, 35~45min is soaked at 0~5 DEG C；
(3) scalping：The above-mentioned cold mixture that carries is crossed into 80~120 mesh sieves, oversize is used for step (6), by the cold of sieve
Extract enters next step (4)；
(4) dusting cover：The cold extract that step (3) is obtained is crossed into 300~400 mesh sieves, oversize is discarded, by the filtrate of sieve
Into next step (5)；
(5) ultrafiltration：By filtrate 0.4~0.6MPa, 20~40 DEG C, by molecular cut off be 5000~10000 it is super
Filter membrane, is not 1 through the dope volume of film and the ratio of stoste volume:8~1:10, after the clear liquid concentrate drying of milipore filter
Mushroom pigment is obtained, mushroom egg is obtained after the dope through milipore filter is not dried using vacuum and low temperature liquid continuous drier
In vain；
(6) hot water extraction：Oversize in step (3) is pressed into solid-liquid mass ratio 1:(4~5) adding distilled water carries out dispensing,
After stirring, the citric acid of dried thin mushroom weight 1.8%~2.2% is added, 55~65min is extracted at 85~95 DEG C, extracted
After end, centrifugation, revolution is 4000~5000r/min, 5~10min of centrifugation time, collects supernatant, and residue is used for secondary carrying
Take, 4~5 times of distilled water of volume of residue are added in residue, second 55~65min of extraction is carried out at 85~95 DEG C, carry
After taking end, centrifugation, revolution is 4000~5000r/min, 5~10min of centrifugation time, collects supernatant, and residue is used for three times
Extract, 3~4 times of distilled water of volume of residue added in residue, third time is carried out at 85~95 DEG C and extracts 55~65min,
After extraction terminates, centrifugation, revolution is 4000~5000r/min, 5~10min of centrifugation time, supernatant is collected, under residue enters
One step drying process, merges the supernatant of above-mentioned three times and crosses 300~400 mesh sieves, is obtained by the filtrate concentrate drying of sieve
Obtain lentinan；
(7) residue is dried：Residue after above-mentioned centrifugation is dried under the conditions of 70~90 DEG C, 60~80 mesh sieves were crushed,
Can obtain mushroom dietary fiber.
Just because of inventor to the well-designed of process above, each step is closely coupled, all linked with one another, just realizes
Dried thin mushroom safety, the comprehensive development and utilization of low cost.Advantage of the invention embodies and formerly goes out to do perfume using low temperature immersing extraction
The materials such as soluble protein and small molecule carbohydrate, pigment in mushroom, impurity content is few in making the lentinan of subsequent extracted, color
It is shallow, it is not necessary to which that adding maltodextrin can be directly spray-dried；In addition, the method can be extracted more than 90% in dried thin mushroom
Lentinan, this active ingredient is fully utilized.Advantage of the invention be also embodied in protein from lentinus edodes separation and
Dry using ultra-filtration and separation concentration and low temperature continuous vacuum belt drying technology, protein from lentinus edodes is separated with small-molecule substance, both
Realize and separate under normal temperature, concentrate, dry protein from lentinus edodes, it is to avoid the decomposition destruction of active component, mushroom can be synchronously realized again
The decolouring of polysaccharide and preliminary purification.
Specific implementation process of the present invention comprises the following steps：
(1) dried thin mushroom is pulverized and sieved, it is desirable to which the grinding particle size of material is between 20~60 mesh；
(2) material after crushing presses solid-liquid mass ratio 1:(20~25) add water carries out dispensing, after stirring, at 0~5 DEG C
35~45min of lower immersion；
(3) the above-mentioned cold mixture that carries is crossed into 80~120 mesh sieves, the water content standard of oversize is：When exerting oneself to hold with hand, nothing
Water oozes out；
(4) the cold extract of excessively complete 80~120 mesh sieve was continued into 300~400 mesh sieves, oversize is discarded；
(5) by the extract solution of excessively complete 300~400 mesh sieve 0.4~0.6MPa, 20~40 DEG C, be by molecular cut off
5000~10000 milipore filter, is not 1 through the dope volume of film and the ratio of stoste volume:8~1:10, through milipore filter
Clear liquid need to be concentrated under reduced pressure at 75~85 DEG C, the volume of concentrate and the ratio of stoste volume are 1:10~1:12, then
In 80~90 DEG C of drying, the mushroom pigment for obtaining is highly soluble in water, and with strong mushroom fragrance, can be used as natural essence
And pigment；The dope through milipore filter is not dried using vacuum and low temperature liquid continuous drier, and temperature control is 25~35
Between DEG C, vacuum is ﹣ 0.095~﹣ 0.099MPa, so that it is guaranteed that the bioactivity of protein from lentinus edodes；
(6) oversize of 80~120 mesh sieves is pressed into solid-liquid mass ratio 1:Plus distilled water carries out dispensing, consolidating now (4~5)
Liquor ratio is the ratio of the weight in wet base with distilled water of oversize；Such as in terms of the weight of dried thin mushroom, dispensing is carried out in following ratio：It is cold
3~4 times of the weight about siccative weight of rear wet feed are carried, Jia 4 again on this basis~5 times of distilled water；After stirring, plus
Enter the citric acid of dried thin mushroom weight 1.8%~2.2%, 55~65min is extracted at 85~95 DEG C, after extraction terminates, centrifugation turns
Number is 4000~5000r/min, 5~10min of centrifugation time, collects supernatant, and residue is used for second extraction, is added in residue
4~5 times of distilled water of volume of residue, carry out second 55~65min of extraction at 85~95 DEG C, and after extraction terminates, centrifugation turns
Number is 4000~5000r/min, 5~10min of centrifugation time, collects supernatant, and residue is used for three extractions, is added in residue
3~4 times of distilled water of volume of residue, third time are carried out at 85~95 DEG C and extract 55~65min, and after extraction terminates, centrifugation turns
Number is 4000~5000r/min, 5~10min of centrifugation time, collects supernatant, and residue enters next step drying process, merges
The supernatant of above-mentioned three times simultaneously crosses 300~400 mesh sieves, is concentrated under reduced pressure at 80~90 DEG C by the filtrate of sieve, concentrate
The ratio of volume and stoste volume be 1:8~1:10, then EAT be 180~190 DEG C, leaving air temp be 90~
It is spray-dried while hot under conditions of 100 DEG C, is obtained lentinan powder；
(7) residue after above-mentioned centrifugation is dried under the conditions of 70~90 DEG C, crushed 60~80 mesh sieves, you can obtain perfume (or spice)
Mushroom dietary fiber.
When the above method is carried out, in order to ensure the permeability of milipore filter, according to specific feelings after using 2~3 cycles
Condition is cleaned to milipore filter.During cleaning, the sample liquid that will first be remained in film with pure water is taken out of, then with the NaOH of pH=11~12
Solution is washed, Stress control in 0.2~0.4MPa, temperature control at 20~40 DEG C, when efflux close to it is colourless when, alkali cleaning
Terminate, then use pure water rinsing.
In sum, advantage of the invention is that can simultaneously be extracted from dried thin mushroom and obtain mushroom pigment, protein from lentinus edodes,
Four kinds of lentinan and mushroom dietary fiber have the product of nutrition and health care function concurrently.First, the mushroom color that the present invention is obtained
Element not only remains in dried thin mushroom 1.5% reduced sugar and 2% oligosaccharide, also with strong mushroom fragrance, can be as
The good source of natural pigment, not only nutrition and health care but also safety；Secondly, the protein from lentinus edodes that the present invention is obtained is to use water at low temperature
The production of technique, membrane filtration and low temperature continuous vacuum belt drying technology is proposed, from existing use alkalinity extraction or using different molten
Agent carries out grading extraction and compares, and extraction time substantially shortens, and extraction efficiency is significantly improved, and bioactivity is remarkably reinforced, and adopts
The protein from lentinus edodes powder obtained with the present invention has good water solubility, can the extensive use in food；Again, the present invention is obtained
Lentinan using step by step arithmetic in short-term, extraction process does not use ethanol, only with the addition of the citric acid of food grade, and spraying is dry
, also without addition maltodextrin, compared with existing method, the polysaccharide lighter color of acquisition, extraction time is short for dry process, extracts
It is complete and safe；Finally, the mushroom dietary fiber that the present invention is obtained remains substantial amounts of fiber, ash content, ore deposit in mushroom
Material and provitamin D, with good healthcare function, in can extensively making an addition to food.The invention not only makes various in mushroom
Nutrition and health care composition has obtained sufficiently effective utilization, and whole technique productions cycle is short, and recovery rate is high, it is cost-effective and
Energy consumption, it is easy to operate, realize the comprehensive development and utilization of mushroom low cost.
Specific embodiment
Following examples are intended to illustrate rather than limitation of the invention further.
Embodiment 1
Dried thin mushroom (perigone mushroom) is crushed to 20 mesh, by solid-liquid mass ratio 1:20 add water carries out dispensing, after stirring,
40min is soaked at 0 DEG C；The cold mixture that carries crosses 100 mesh sieves, and the water content standard of stand-by oversize is：When exerting oneself to hold with hand, nothing
Water oozes out；Filtered solution continued 300 mesh sieves, and oversize is discarded；Filtered solution 0.5MPa, 40 DEG C, be by molecular cut off
5000 milipore filter, is not 1 through the dope volume of film and the ratio of stoste volume:8, the clear liquid through milipore filter enters at 80 DEG C
Row is concentrated under reduced pressure, and the volume of concentrate is 1 with the ratio of stoste volume:10, then in 85 DEG C of drying, you can obtain mushroom color
Element.The pigment is highly soluble in water, and with strong mushroom fragrance, the aqueous solution is shiny red, and the pigment remains perfume (or spice) after measured
1.43% reduced sugar, 2.08% oligosaccharide in mushroom dry powder, can be used as natural essence and pigment；Not through the dense of milipore filter
Liquid is dried using the vacuum and low temperature liquid continuous drier that Shanghai Minjie Machinery Co. Ltd. develops, and temperature of charge is 30
DEG C, vacuum is ﹣ 0.098MPa, and the protein from lentinus edodes powder of acquisition has good instant capacity, and yield is 8.6%, after testing, is contained
There are 34.2% protein, 3.1% soluble dietary fiber, 1.8% insoluble diedairy fiber.
The stand-by oversize of 100 mesh sieves is pressed into solid-liquid mass ratio 1:4.5 plus distilled water carry out dispensing, solid-to-liquid ratio now
It is the ratio of the weight in wet base with distilled water of oversize；Such as in terms of the weight of dried thin mushroom, dispensing is carried out in following ratio：It is cold carry after
The weight of wet feed is 3.3 times of siccative weight, adds 4.5 times of distilled water again on this basis；After stirring, dried thin mushroom is added
The citric acid of weight 1.9%, 60min is extracted at 90 DEG C, after extraction terminates, centrifugation, and revolution is 4000r/min, centrifugation time
5min, collects clear liquid, and residue is used for second extraction, and 4 times of distilled water of volume of residue are added in residue, is extracted at 90 DEG C
60min, after extraction terminates, centrifugation, revolution is 4000r/min, centrifugation time 5min, collects clear liquid, and residue is used for three extractions,
3 times of distilled water of volume of residue are added in residue, 60min is extracted at 90 DEG C, after extraction terminates, be centrifuged, revolution is
4000r/min, centrifugation time 5min, collect clear liquid, merge the supernatant of three times and cross 300 mesh sieves, and the clear liquid after sieving is existed
85 DEG C are concentrated under reduced pressure, and the volume of concentrate is 1 with the ratio of stoste volume:8, it is then 180 DEG C, air-out in EAT
Temperature obtains flaxen lentinan powder to be spray-dried while hot under conditions of 90 DEG C, and yield is 13.3%, through inspection
Survey, polyoses content is 43%, insoluble diedairy fiber content is 0.28%.Residue after above-mentioned third time is centrifuged is in 70 DEG C of bars
Dried under part, crushed 60 mesh sieves, you can obtain mushroom dietary fiber, after testing, its polyoses content is 0.65%, show dry perfume
More than 90% soluble saccharide is efficiently separated in mushroom.By the technique, the active component in mushroom has obtained different journeys
The enrichment of degree, forms various products with nutrition and health care function, realizes mushroom comprehensive utilization and maximizes.
Embodiment 2
Dried thin mushroom (937) is crushed to 40 mesh, by solid-liquid mass ratio 1:25 add water carries out dispensing, after stirring, at 4 DEG C
Lower immersion 35min；The cold mixture that carries crosses 80 mesh sieves, and the water content standard of stand-by oversize is：It is anhydrous to ooze when exerting oneself to hold with hand
Go out；Filtered solution continued 300 mesh sieves, and oversize is discarded；Filtered solution 0.5MPa, 35 DEG C, be 5000 by molecular cut off
Milipore filter, is not 1 through the dope volume of film and the ratio of stoste volume:9,85 DEG C of the clear liquid through milipore filter carries out depressurizing dense
Contracting, the volume of concentrate is 1 with the ratio of stoste volume:11, then in 90 DEG C of drying, you can obtain mushroom pigment.The pigment
Water is highly soluble in, and with strong mushroom fragrance, the aqueous solution is shiny red, during the pigment remains mushroom dry powder after measured
1.49% reduced sugar, 2.18% oligosaccharide, can be used as natural essence and pigment；The dope through milipore filter does not use upper
The vacuum and low temperature liquid continuous drier that Hai Minjie Machinery Co., Ltd.s develop is dried, and temperature of charge is 30 DEG C, vacuum
It is ﹣ 0.098MPa, the protein from lentinus edodes powder of acquisition has good instant capacity, and yield is 8.9%, after testing, contains 35.8%
Protein, 3.4% soluble dietary fiber, 1.9% insoluble diedairy fiber.
The stand-by oversize of 80 mesh sieves is pressed into solid-liquid mass ratio 1:5 plus distilled water carry out dispensing, solid-to-liquid ratio now is
The weight in wet base of oversize and the ratio of distilled water；Such as in terms of the weight of dried thin mushroom, dispensing is carried out in following ratio：It is cold to carry rear wet
The weight of material is 3.8 times of siccative weight, adds 5 times of distilled water again on this basis；After stirring, dried thin mushroom weight is added
2% citric acid, 60min is extracted at 95 DEG C, and after extraction terminates, centrifugation, revolution is 4500r/min, centrifugation time 8min, is collected
Clear liquid, residue is used for second extraction, and 4.5 times of distilled water of volume of residue are added in residue, and 60min is extracted at 95 DEG C, carries
After taking end, centrifugation, revolution is 4500r/min, centrifugation time 8min, collects clear liquid, and residue is used for three extractions, in residue
3.5 times of distilled water of volume of residue are added, 60min is extracted at 95 DEG C, after extraction terminates, centrifugation, revolution is 4500r/min,
Centrifugation time 8min, collects clear liquid, merges the supernatant of three times and crosses 300 mesh sieves, and the clear liquid after sieving is subtracted at 80 DEG C
Pressure concentration, the volume of concentrate is 1 with the ratio of stoste volume:9, then EAT be 190 DEG C, leaving air temp be 95 DEG C
Under conditions of be spray-dried while hot, obtain lentinan powder, yield is 13.8%, and after testing, polyoses content is 45%,
Insoluble diedairy fiber content is 0.33%.Residue after above-mentioned third time is centrifuged is dried under the conditions of 80 DEG C, crushes 80
Mesh sieve, you can obtain mushroom dietary fiber, after testing, its polyoses content is 0.61%, show in dried thin mushroom more than 90% can
Dissolubility carbohydrate is efficiently separated.By the technique, the active component in mushroom has obtained different degrees of enrichment, is formed various
Product with nutrition and health care function, realizes mushroom comprehensive utilization and maximizes.
Embodiment 3
Dried thin mushroom (937) is crushed to 60 mesh, by solid-liquid mass ratio 1:25 add water carries out dispensing, after stirring, at 4 DEG C
Lower immersion 45min；The cold mixture that carries crosses 120 mesh sieves, and the water content standard of stand-by oversize is：It is anhydrous when exerting oneself to hold with hand
Ooze out；Filtered solution continued 300 mesh sieves, and oversize is discarded；Filtered solution 0.6MPa, 35 DEG C, by molecular cut off be 10000
Milipore filter, the ratio through dope volume and the stoste volume of film is 1:10,80 DEG C of the clear liquid through milipore filter is subtracted
Pressure concentration, the volume of concentrate is 1 with the ratio of stoste volume:12, then in 80 DEG C of drying, you can obtain mushroom pigment.Should
Pigment is highly soluble in water, and with strong mushroom fragrance, the aqueous solution is shiny red, and the pigment remains mushroom dry powder after measured
In 1.53% reduced sugar, 2.24% oligosaccharide, can be used as natural essence and pigment；The dope through milipore filter is not used
The vacuum and low temperature liquid continuous drier that Shanghai Minjie Machinery Co. Ltd. develops is dried, and temperature of charge is 30 DEG C, vacuum
It is ﹣ 0.098MPa to spend, and the protein from lentinus edodes powder of acquisition has good instant capacity, and yield is 9.1%, after testing, is contained
36.7% protein, 3.1% soluble dietary fiber, 1.7% insoluble diedairy fiber.
The stand-by oversize of 120 mesh sieves is pressed into solid-liquid mass ratio 1:4 plus distilled water carry out dispensing, solid-to-liquid ratio now is
The weight in wet base of oversize and the ratio of distilled water；Such as in terms of the weight of dried thin mushroom, dispensing is carried out in following ratio：It is cold to carry rear wet
The weight of material is 4 times of siccative weight, adds 4 times of distilled water again on this basis；After stirring, dried thin mushroom weight is added
2.2% citric acid, 60min are extracted at 90 DEG C, after extraction terminates, centrifugation, and revolution is 5000r/min, centrifugation time 10min,
Clear liquid is collected, residue is used for second extraction, and 4 times of distilled water of volume of residue are added in residue, and 60min is extracted at 90 DEG C,
After extraction terminates, centrifugation, revolution is 5000r/min, centrifugation time 10min, collects clear liquid, and residue is used for three extractions, in residual
4 times of distilled water of volume of residue are added in slag, 60min is extracted at 90 DEG C, after extraction terminates, centrifugation, revolution is 5000r/
Min, centrifugation time 10min, collect clear liquid, merge the supernatant of three times and cross 300 mesh sieves, and the clear liquid after sieving is entered at 90 DEG C
Row is concentrated under reduced pressure, and the volume of concentrate is 1 with the ratio of stoste volume:10, it is then 180 DEG C, leaving air temp in EAT
To be spray-dried while hot under conditions of 100 DEG C, lentinan powder is obtained, yield is 14.1%, after testing, polyoses content
It is 46%, insoluble diedairy fiber content is 0.37%.Residue after above-mentioned third time is centrifuged is dried under the conditions of 90 DEG C, powder
The mesh sieve of broken mistake 80, you can obtain mushroom dietary fiber, after testing, its polyoses content is 0.66%, show in dried thin mushroom 90% with
On soluble saccharide efficiently separated.By the technique, the active component in mushroom has obtained different degrees of enrichment, shape
Into various products with nutrition and health care function, realize mushroom comprehensive utilization and maximize.
The use that the present invention passes through the physical methods such as low temperature, high temperature, membrane filtration, realizes the comprehensive profit of mushroom low cost
With, the bioactivity of each extract is maintained to greatest extent, its security is in turn ensure that, pigment, the egg produced by the method
In vain, polysaccharide, dietary fiber can be directly appended in food, not only nutrition and health care but also safety.Secondly by the mushroom that the method is produced
Pigment is highly soluble in water and has strong mushroom fragrance, wherein containing 1.5% reduced sugar, 2% oligosaccharide in dried thin mushroom,
Can be used as natural essence and pigment；The protein from lentinus edodes produced by the method has good instant capacity, and yield is 9% or so,
Wherein protein content is 35%, and soluble dietary fibre content is 3.3%, and insoluble diedairy fiber content is 1.8%；Pass through
The lentinan of method production is pale yellow powder, and yield is 14% or so, and its polyoses content is 45%, insoluble diedairy fiber
Content be 0.3%；Residue after extraction can be only as the good source of dietary fiber, the content of wherein soluble polysaccharide
0.6%, by the technique, in dried thin mushroom more than 90% polysaccharide can be extracted, and formed and various have nutrition and health care work(concurrently
The product of energy, this can not only reduce the production cost of mushroom deep processing enterprise, improve value-added content of product, and can promote
Mushroom planting industry and the development of food service industry.
Claims (6)
1. a kind of method that utilization dried thin mushroom produces pigment, albumen, polysaccharide and dietary fiber, it is characterised in that including following step
Suddenly：
(1) dried thin mushroom is crushed：Dried thin mushroom is pulverized and sieved, it is desirable to which the grinding particle size of material is between 20~60 mesh；
(2) cold water extraction：Material after crushing is pressed into solid-liquid mass ratio 1:(20~25) add water carries out dispensing, after stirring,
35~45min is soaked at 0~5 DEG C；
(3) scalping：The above-mentioned cold mixture that carries is crossed into 80~120 mesh sieves, oversize is used for step (6), by the cold extract of sieve
Into next step (4)；
(4) dusting cover：The cold extract that step (3) is obtained is crossed into 300~400 mesh sieves, oversize is discarded, entered by the filtrate of sieve
Next step (5)；
(5) ultrafiltration：By filtrate 0.4~0.6MPa, 20~40 DEG C, by the milipore filter that molecular cut off is 5000~10000,
It is not 1 through the dope volume of film and the ratio of stoste volume:8~1:10, obtained through after the clear liquid concentrate drying of milipore filter
Mushroom pigment, not the dope through milipore filter obtain protein from lentinus edodes after being dried using vacuum and low temperature liquid continuous drier；
(6) hot water extraction：Oversize in step (3) is pressed into solid-liquid mass ratio 1:(4~5) adding distilled water carries out dispensing, stirs
After uniform, the citric acid of dried thin mushroom weight 1.8%~2.2% is added, 55~65min is extracted at 85~95 DEG C, extraction terminates
Afterwards, it is centrifuged, revolution is 4000~5000r/min, 5~10min of centrifugation time, collects supernatant, residue is used for second extraction, in
4~5 times of distilled water of volume of residue are added in residue, second 55~65min of extraction is carried out at 85~95 DEG C, extraction terminates
Afterwards, it is centrifuged, revolution is 4000~5000r/min, 5~10min of centrifugation time, collects supernatant, residue is used for three extractions, in
3~4 times of distilled water of volume of residue are added in residue, third time is carried out at 85~95 DEG C and is extracted 55~65min, extraction terminates
Afterwards, it is centrifuged, revolution is 4000~5000r/min, 5~10min of centrifugation time, collects supernatant, residue is dry into next step
Dry process, merges the supernatant of above-mentioned three times and crosses 300~400 mesh sieves, and it is more to obtain mushroom by the filtrate concentrate drying of sieve
Sugar；
(7) residue is dried：Residue after above-mentioned centrifugation is dried under the conditions of 70~90 DEG C, 60~80 mesh sieves were crushed, you can
Obtain mushroom dietary fiber.
2., as claimed in claim 1 using the method for dried thin mushroom production pigment, albumen, polysaccharide and dietary fiber, its feature exists
In：Dope in step (5) not through milipore filter need to be dried using vacuum and low temperature liquid continuous drier, whole dried
Journey, temperature of charge is controlled between 25~35 DEG C, and vacuum so may insure protein from lentinus edodes in ﹣ 0.095~﹣ 0.099MPa
Bioactivity.
3., as claimed in claim 1 using the method for dried thin mushroom production pigment, albumen, polysaccharide and dietary fiber, its feature exists
In：The supernatant of three extractions is crossed after 300~400 mesh sieves in step (6), need to be concentrated under reduced pressure at 80~90 DEG C, is concentrated
The volume of liquid is 1 with the ratio of stoste volume:8~1:10, then in 180~190 DEG C of EAT, leaving air temp 90~100
It is spray-dried under conditions of DEG C, is obtained lentinan powder.
4., as claimed in claim 3 using the method for dried thin mushroom production pigment, albumen, polysaccharide and dietary fiber, its feature exists
In：Lentinan concentrate need to spray while hot when being spray-dried.
5., as claimed in claim 1 using the method for dried thin mushroom production pigment, albumen, polysaccharide and dietary fiber, its feature exists
In：Described dried thin mushroom is various models, the mushroom of various qualities of greenhouse plantation.
6., as claimed in claim 5 using the method for dried thin mushroom production pigment, albumen, polysaccharide and dietary fiber, its feature exists
In：Described dried thin mushroom is 937 and/or perigone mushroom.
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| CN201410535782.9A CN104829734B (en) | 2014-10-10 | 2014-10-10 | A kind of method of utilization dried thin mushroom production pigment, albumen, polysaccharide and dietary fiber |
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| CN104829734B true CN104829734B (en) | 2017-06-13 |
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| CN105399853A (en) * | 2015-12-16 | 2016-03-16 | 黑龙江众生生物工程有限公司 | Production method for extracting various shiitake mushroom effective substances from shiitake mushroom stipe in one time |
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| CN106520422A (en) * | 2016-10-08 | 2017-03-22 | 福建农林大学 | Anti-aging mushroom concentrated beverage and preparation method thereof |
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| CN107581616A (en) * | 2017-08-18 | 2018-01-16 | 武汉华士特工业生物技术开发有限公司 | A kind of method that protein from lentinus edodes and mushroom oligosaccharide are synchronously prepared using mushroom |
| CN109043495A (en) * | 2018-07-25 | 2018-12-21 | 辽宁省农业科学院 | A kind of preparation method of edible fungi root extract |
| CN110054708B (en) * | 2019-04-30 | 2021-06-18 | 容益(海南)农业开发有限公司 | Edible mushroom extract concentrating and drying integrated technology |
| CN110483653B (en) * | 2019-07-16 | 2022-03-18 | 南昌大学 | Preparation method of lentinan component with immunocompetence, product and application |
| CN114990184A (en) * | 2022-06-16 | 2022-09-02 | 陕西科技大学 | Green co-production preparation method of edible fungus glucan, polypeptide and crude fiber |
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