CN103709262B - A kind of extracting method of highly purified Auricularia polysaccharide - Google Patents
A kind of extracting method of highly purified Auricularia polysaccharide Download PDFInfo
- Publication number
- CN103709262B CN103709262B CN201310630767.8A CN201310630767A CN103709262B CN 103709262 B CN103709262 B CN 103709262B CN 201310630767 A CN201310630767 A CN 201310630767A CN 103709262 B CN103709262 B CN 103709262B
- Authority
- CN
- China
- Prior art keywords
- thick paste
- extraction
- lixiviate
- auricularia
- extracting method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The present invention relates to a kind of extracting method of highly purified Auricularia polysaccharide, belong to technical field of natural product extraction.Comprise the steps: to get auricularia auriculajudae, after drying, grind into powder; Add aqueous ethanolic solution in the powder, lixiviate, after removing solids, obtain vat liquor, then vat liquor reclaim under reduced pressure is become thick paste; Thick paste is lowered the temperature, then in thick paste, adds water, generation is precipitated, supernatant liquid is removed, obtains precipitation; In precipitation, add ethyl acetate and water, extraction, abandons extraction phase, and collected by aqueous phase, carry out concentrating under reduced pressure, after obtaining concentrated solution, spraying dry, to obtain final product.The extracting method of Auricularia polysaccharide provided by the invention, operational condition is gentle, and raw material is easy to get, and technique is simple, and yield is high, and purity is good.
Description
Technical field
The present invention relates to a kind of extracting method of Auricularia polysaccharide, particularly relate to a kind of extracting method of Auricularia polysaccharide of higher degree, belong to technical field of natural product extraction.
Background technology
Generally refer to from the polysaccharide composition in large edible or medicinal fungi.The important physiological function of fungus polysaccharide includes: (1) is produced by activated macrophage, stimulation antibody, improves human immunological competence; (2) there is strong anti-tumor activity, have very strong restraint to cancer cells; (3) other effect: anti-ageing, hypoglycemic, reducing blood-fat, antithrombotic, protect the liver, promote that protein and nucleic acid synthesize, increase white corpuscle-radioresistance, antiulcer agent, anti-inflammatory, anticoagulation, enhancing marrow hemopoietic function.The food (medicine) studying report in recent years has four classes with granulose: mixed polysaccharide (Heteropolysaccharide), mannosans (Mannan), dextran (Glucan), glycoprotein (Glycoprotein) and polysaccharide peptide (Polysaccharidepeptide).
Auricularia auriculajudae, another name black fungus, brightness program, being Mycophyta Basidiomycetes Auriculariaceae Auricularia plant, is the Jelly fungi of the dietotherapeutic of China's preciousness, general single raw or all living creatures.Current cultivation based on linden with Bag Material.Black fungus is traditional edible medicinal fungus that Chinese people like, not only meat is fine and smooth, crisp smooth mouth, and it is nutritious, containing protein, fat, carbohydrate and multivitamin and inorganic salt, be indispensable black-food during people live, be called as " meat or fish in element ", have the title of " black national treasure ".
The extracting method of Auricularia polysaccharide mainly contains: Hot water extraction, diluted alkaline extraction, zymohydrolysis extracting method etc.Although these methods are comparatively easy, the purity of the polysaccharide obtained is not high general about 10% ~ 20%.
Summary of the invention
The object of the invention is: the extracting method providing a kind of high purity Auricularia polysaccharide, have employed following technical scheme:
An extracting method for highly purified Auricularia polysaccharide, comprises the steps:
1st step, by weight, get 50 ~ 70 parts, auricularia auriculajudae, after drying, grind into powder;
2nd step, add the aqueous ethanolic solution of 5 ~ 8 times of weight in the powder, lixiviate, after removing solids, obtain vat liquor, then vat liquor reclaim under reduced pressure is become thick paste;
3rd step, thick paste is cooled to 2 ~ 4 DEG C, in thick paste, then adds the water of 200 ~ 300 parts, generation is precipitated, supernatant liquid is removed, obtains precipitation;
4th step, adds ethyl acetate 30 ~ 50 parts and 40 ~ 60 parts, water in precipitation, and extraction, abandons extraction phase, and collected by aqueous phase, carry out concentrating under reduced pressure, after obtaining concentrated solution, spraying dry, to obtain final product.
Preferably, the mass concentration of the aqueous ethanolic solution in the 2nd step is 50 ~ 70%.More excellent is 60%.
Preferably, the temperature of the lixiviate in the 2nd step is 65 ~ 75 DEG C.
Preferably, the time of the lixiviate in the 2nd step is 2 ~ 4 hours.
Preferably, spray-dired inlet temperature 150 ~ 160 DEG C in the 4th step, air outlet temperature 70 ~ 80 DEG C.
In method provided by the invention, the object of the 1st step is that auricularia auriculajudae is broken, active substance in fact can be made to be leached better, the effect that 2nd step adds ethanol is the precipitation promoting active substance, the effect of the 3rd step is the mode by depositing in water, and active substance is precipitated, and can be separated with other composition, the effect of the 4th step by the mode of extraction, some compositions be dissolved in organic phase is extracted to take away mutually, improves the purity of polysaccharide.
beneficial effect
The extracting method of Auricularia polysaccharide provided by the invention, operational condition is gentle, and raw material is easy to get, and technique is simple, and yield is high, and purity is good.
Embodiment
Embodiment 1
1st step, get auricularia auriculajudae 50Kg, after drying, grind into powder;
2nd step, add the aqueous ethanolic solution of 5 times of weight in the powder, lixiviate, after removing solids, obtain vat liquor, then vat liquor reclaim under reduced pressure is become thick paste;
3rd step, thick paste is cooled to 2 DEG C, in thick paste, then adds the water of 200Kg, generation is precipitated, supernatant liquid is removed, obtains precipitation;
4th step, adds ethyl acetate 30Kg and water 40Kg in precipitation, and extraction, abandons extraction phase, and collected by aqueous phase, carry out concentrating under reduced pressure, after obtaining concentrated solution, spraying dry, to obtain final product.
The mass concentration of the aqueous ethanolic solution in the 2nd step is 50%, and the temperature of the lixiviate in the 2nd step is 65 DEG C, and the time of the lixiviate in the 2nd step is 2 hours, spray-dired inlet temperature 150 DEG C in the 4th step, air outlet temperature 70 DEG C.
Test with reference to polysaccharide detection method under the glossy ganoderma recorded in Chinese Pharmacopoeia 2010 editions or sealwort item, the content of the Auricularia polysaccharide obtained is 32%.
Embodiment 2
1st step, get auricularia auriculajudae 70Kg, after drying, grind into powder;
2nd step, add the aqueous ethanolic solution of 8 times of weight in the powder, lixiviate, after removing solids, obtain vat liquor, then vat liquor reclaim under reduced pressure is become thick paste;
3rd step, thick paste is cooled to 4 DEG C, in thick paste, then adds the water of 300Kg, generation is precipitated, supernatant liquid is removed, obtains precipitation;
4th step, adds ethyl acetate 50Kg and water 60Kg in precipitation, and extraction, abandons extraction phase, and collected by aqueous phase, carry out concentrating under reduced pressure, after obtaining concentrated solution, spraying dry, to obtain final product.
The mass concentration of the aqueous ethanolic solution in the 2nd step is 70%, and the temperature of the lixiviate in the 2nd step is 75 DEG C, and the time of the lixiviate in the 2nd step is 4 hours, spray-dired inlet temperature 160 DEG C in the 4th step, air outlet temperature 80 DEG C.
Test with reference to polysaccharide detection method under the glossy ganoderma recorded in Chinese Pharmacopoeia 2010 editions or sealwort item, the content of the Auricularia polysaccharide obtained is 34%.
Embodiment 3
1st step, get auricularia auriculajudae 60Kg, after drying, grind into powder;
2nd step, add the aqueous ethanolic solution of 6 times of weight in the powder, lixiviate, after removing solids, obtain vat liquor, then vat liquor reclaim under reduced pressure is become thick paste;
3rd step, thick paste is cooled to 3 DEG C, in thick paste, then adds the water of 250Kg, generation is precipitated, supernatant liquid is removed, obtains precipitation;
4th step, adds ethyl acetate 40Kg and water 50Kg in precipitation, and extraction, abandons extraction phase, and collected by aqueous phase, carry out concentrating under reduced pressure, after obtaining concentrated solution, spraying dry, to obtain final product.
The mass concentration of the aqueous ethanolic solution in the 2nd step is 60%, and the temperature of the lixiviate in the 2nd step is 70 DEG C, and the time of the lixiviate in the 2nd step is 3 hours, spray-dired inlet temperature 155 DEG C in the 4th step, air outlet temperature 74 DEG C.
Test with reference to polysaccharide detection method under the glossy ganoderma recorded in Chinese Pharmacopoeia 2010 editions or sealwort item, the content of the Auricularia polysaccharide obtained is 36%.
Claims (1)
1. an extracting method for highly purified Auricularia polysaccharide, comprises the steps:
1st step, get auricularia auriculajudae 60Kg, after drying, grind into powder;
2nd step, add the aqueous ethanolic solution of 6 times of weight in the powder, lixiviate, after removing solids, obtain vat liquor, then vat liquor reclaim under reduced pressure is become thick paste;
3rd step, thick paste is cooled to 3 DEG C, in thick paste, then adds the water of 250Kg, generation is precipitated, supernatant liquid is removed, obtains precipitation;
4th step, adds ethyl acetate 40Kg and water 50Kg in precipitation, and extraction, abandons extraction phase, and collected by aqueous phase, carry out concentrating under reduced pressure, after obtaining concentrated solution, spraying dry, to obtain final product;
The mass concentration of the aqueous ethanolic solution in the 2nd step is 60%, and the temperature of the lixiviate in the 2nd step is 70 DEG C, and the time of the lixiviate in the 2nd step is 3 hours, spray-dired inlet temperature 155 DEG C in the 4th step, air outlet temperature 74 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310630767.8A CN103709262B (en) | 2013-12-02 | 2013-12-02 | A kind of extracting method of highly purified Auricularia polysaccharide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310630767.8A CN103709262B (en) | 2013-12-02 | 2013-12-02 | A kind of extracting method of highly purified Auricularia polysaccharide |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103709262A CN103709262A (en) | 2014-04-09 |
| CN103709262B true CN103709262B (en) | 2016-02-10 |
Family
ID=50402646
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310630767.8A Active CN103709262B (en) | 2013-12-02 | 2013-12-02 | A kind of extracting method of highly purified Auricularia polysaccharide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103709262B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105273102A (en) * | 2015-11-03 | 2016-01-27 | 广西南宁胜祺安科技开发有限公司 | Method for extracting auricularia auricula polysaccharide from auricularia auricula |
| CN105728431A (en) * | 2016-02-24 | 2016-07-06 | 齐芳 | Cleaning technology for vinasse |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1580079A (en) * | 2004-05-21 | 2005-02-16 | 大连轻工业学院 | Method for extracting edible tree fungi polysaccharide |
| CN102060934A (en) * | 2010-12-11 | 2011-05-18 | 中国计量学院 | Enzymatic extraction method for auricularia auricula polysaccharides |
| CN102321189A (en) * | 2011-10-24 | 2012-01-18 | 周伯扬 | Comprehensive extraction process for auricularia auricula polysaccharide |
| CN102585032A (en) * | 2012-03-09 | 2012-07-18 | 武汉大学 | Water-soluble polysaccharide extracted from black fungus and preparation method thereof |
| CN102827302A (en) * | 2012-09-07 | 2012-12-19 | 龙海市颖欣农业科技有限公司 | Preparation method of auricularia auricular polysaccharides |
-
2013
- 2013-12-02 CN CN201310630767.8A patent/CN103709262B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1580079A (en) * | 2004-05-21 | 2005-02-16 | 大连轻工业学院 | Method for extracting edible tree fungi polysaccharide |
| CN102060934A (en) * | 2010-12-11 | 2011-05-18 | 中国计量学院 | Enzymatic extraction method for auricularia auricula polysaccharides |
| CN102321189A (en) * | 2011-10-24 | 2012-01-18 | 周伯扬 | Comprehensive extraction process for auricularia auricula polysaccharide |
| CN102585032A (en) * | 2012-03-09 | 2012-07-18 | 武汉大学 | Water-soluble polysaccharide extracted from black fungus and preparation method thereof |
| CN102827302A (en) * | 2012-09-07 | 2012-12-19 | 龙海市颖欣农业科技有限公司 | Preparation method of auricularia auricular polysaccharides |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103709262A (en) | 2014-04-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104829734B (en) | A kind of method of utilization dried thin mushroom production pigment, albumen, polysaccharide and dietary fiber | |
| CN104757252B (en) | A kind of preparation method of the grifola frondosus protein zymolyte with antioxidation activity | |
| CN104480162A (en) | Method for efficiently extracting hericium erinaceus polysaccharides | |
| CN106213523A (en) | A kind of extracting method of Salicornia Bigelovii Torr. dietary fiber | |
| CN102093598A (en) | Composite edible fungi polysaccharides and preparation method thereof | |
| CN109022527A (en) | A kind of quinoa polypeptide and preparation method thereof with hypotensive activity | |
| CN103976411B (en) | A kind of manufacture method of product of delivering vegetables | |
| CN106962590A (en) | A kind of method for preparing edible fungus protein powder | |
| CN104017098A (en) | Method for directly extracting lentinus-edodes active polysaccharide from waste lentinus-edodes sticks | |
| CN103976413A (en) | Continuous extraction method of protein and meal fiber from walnut meal | |
| CN106946997B (en) | The longan pulp polysaccharide of functional activity enhancing and its green modification method for preparing combined based on endogenous protein | |
| CN102512353A (en) | Dictyophora indusiata water extract and preparation method and application thereof | |
| CN105924495A (en) | Efficient preparing method for high-purity flaxseed protein | |
| CN103709262B (en) | A kind of extracting method of highly purified Auricularia polysaccharide | |
| CN106174475B (en) | A kind of selenoprotein holoside nutrient composite powder and preparation method thereof | |
| CN105559068B (en) | It is a kind of to utilize composition for eating the acquisition of medicine fungi fermentation radix tetrastigme and preparation method thereof | |
| CN107115228A (en) | A kind of sea urchin polysaccharide moisturizing muffin and preparation method thereof | |
| CN109965075A (en) | A method of extracting selenoprotein from selenium-enriched edible mushroom | |
| CN108624636A (en) | A kind of preparation method of lentinan | |
| CN105433368B (en) | A kind of improvement mushroom taste is easy to the edible mushroom composite nutritional tablet being absorbed and utilized and preparation method thereof | |
| CN108841200B (en) | A kind of preparation method of water solubility agaric melanin | |
| CN104403020A (en) | Semi-bionic lentinan extraction method | |
| CN104277139A (en) | Method for extracting flammulina velutipes polysaccharide | |
| CN105175572A (en) | High-purity agaric polysaccharide extracting method | |
| CN104926957B (en) | Method of extracting polysaccharides from Pleurotus eryngii |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210427 Address after: 226300 WuJie central bridge, Tongzhou District, Nantong City, Jiangsu Province Patentee after: Nantong Tongzhou District WuJie agricultural electromechanical management station Address before: 226300, Jiangsu Nantong hi tech Zone, Century Road, No. 788 East Science Park, building of excellence Patentee before: NEOVOLTAIC ENERGY NANTONG Co.,Ltd. |
|
| TR01 | Transfer of patent right |