CN102093598A - Composite edible fungi polysaccharides and preparation method thereof - Google Patents
Composite edible fungi polysaccharides and preparation method thereof Download PDFInfo
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Abstract
The invention relates to composite edible fungi polysaccharides and a preparation method thereof. The preparation method comprises the following steps: selecting fruiting bodies of Lentinus edodes, Auricularia aurlcula, Ganoderma lucidum and Grifola frondosa as raw materials; weighing at a ratio of (1:2):(2:1), mixing, pulverizing, adding distilled water, homogeneously mixing, and sequentially performing microwave extraction and condensation reflux extraction; separating residues and liquid; adding residues into distilled water, and sequentially performing microwave extraction and condensation reflux extraction for 1-2 times; mixing extractive solutions, carrying out vacuum concentrating until the volume of the extractive solution is 1/3-1/4 of the original volume; and precipitating with alcohols, vacuum drying, and pulverizing to obtain composite edible fungi polysaccharides. The composite edible fungi polysaccharides provided by the invention have good physiological activity and complete efficacy, and has the functions of resisting viruses, reducing blood lipid, regulating immune system, resisting platelet aggregation, resisting tumor, protecting liver, resisting gene mutation and resisting acquired immunodeficiency syndrome (AIDS) and the like. The preparation method of the composite edible fungi polysaccharides has the advantages of high yield of polysaccharides, reasonable process, and high operability.
Description
Technical field
The present invention relates to the preparation of polysaccharide, especially a kind of composite edible granulose and preparation method thereof.
Background technology
Along with the improvement of people's living condition and the quickening of rhythm of life, nutrition of edible fungi is worth and medicinal efficacy is familiar with by people gradually.We know, edible mushrooms is a kind ofly to have of a great variety, biomass and greatly, easily cultivate and nutritious microorganism, very wide in distributed in nature, comprise mushroom, straw mushroom, mushroom, auricularia auriculajudae, white fungus, hedgehog hydnum, dictyophora phalloidea, matsutake, dried mushroom, russule and bolete etc.; Contain abundant polysaccharide material in the edible mushrooms, its polysaccharide is mostly based on dextran, as has a β-1,2-glucoside bond bonded straight chain and β-1,3-glucoside bond bonded side chain constitutes, and has special space structure, is the multiple major function composition of edible mushrooms, the physiologically active that it is good, system plays an important role to human immunity.Pharmacological experiments shows: edible fungi polysaccharide not only has anti-inflammatory, anticoagulation, antiviral, reducing blood-fat, reducing cholesterol, prevent arteriosclerosis, function such as delay senility, also having but also multiple carcinogenic, short cancer thing is had restraining effect, is a kind of natural green series products with specific function.
At present, the edible fungi polysaccharide product on the market is single or small amount of matter is formed, and the effect that is played is single relatively.For example lentinan, ganoderan, grifolan, Cordyceps polysaccharide, mushroom polysaccharide, flammulina velutipes, hericium erinaceum polysaccharide, Agaricus Blazei Murrill polysaccharide, Auricularia polycose, tea tree mushroom polysaccharide, lycium barbarum polysaccharide etc.In open file, the preparation technology of its polysaccharide also proposes at single polysaccharide product, as, in disclosed " a kind of preparation method of shiitake fungus polyoses oral liquid " on August 20th, the 2008 disclosed CN101244093A Chinese invention patent Shen Qing Publication specification sheets, January in 2009 Granted publication CN100453559C on the 21st Chinese invention patent specification sheets disclosed " a kind of method of extracting lentinan "; And for example, on March 28th, 2007 Granted publication CN1307314C Chinese invention patent specification sheets in disclosed " enzymolysis prepares the method for ganoderan ", on February 18th, 2009 bulletin CN101367881A Chinese invention patent Shen Qing Publication specification sheets in disclosed " a kind of high efficiency preparation method of ganoderan "; And for example, in disclosed " a kind of Blackfungus polyhexose extraction method " in the disclosed CN101514234A Chinese invention patent Shen Qing Publication specification sheets on August 26th, 2009, the 20106 months 16 days disclosed CN101736055A Chinese invention patent Shen Qing Publication specification sheetss disclosed " a kind of extracting method of Auricularia polycose "; For another example, on June 4th, 2003 Granted publication CN1110566C Chinese invention patent specification sheets in disclosed " process for extracting polyose from Polyporus frondosus fermenting liquor ", on June 20th, 2007 Granted publication CN1322141C Chinese invention patent specification sheets in disclosed " method of preparing huishuhua (grey tree flower) polysaccharide by enzymolysis ".
Summary of the invention
In order to overcome the single relatively deficiency of edible fungi polysaccharide product efficacy in the prior art, the invention provides that a kind of physiologically active is good, effect is comprehensive, solvability well easily absorbs, preparation technology is reasonable, polysaccharide yield height, workable composite edible granulose and preparation method thereof.
The present invention solves the technical scheme that its technical problem takes: a kind of preparation method of composite edible granulose is characterized in that: through following technology
The selection of a, raw material is to choose mushroom, black fungus, glossy ganoderma and Grifola frondosa to do sporophore be raw material with handling it, it is weighed in proportion mix the back and pulverize, and adds an amount of distilled water and mixes, and gets the mixture of edible mushrooms powder and water;
The mixture that b, microwave and condensing reflux extract edible mushrooms powder that step a is obtained and water carries out microwave extraction, condensing reflux extraction successively;
Carry out slag, liquid separation after c, slag, liquid separation and Extraction finish, get extracting solution and residue;
D, microwave and condensing reflux extract an amount of distilled water of residue adding that step c is obtained again and mix, and carry out microwave extraction and condensing reflux more successively and extract 1-2 time, respectively carry out slag, liquid separation, get extracting solution and residue;
E, extracting solution concentrate the extracting solution merging that step c and steps d are obtained, and vacuum concentration must extract concentrated solution to the 1/3-1/4 of original volume;
F, alcohol precipitation add ethanol with the extraction concentrated solution that step e obtains, and leave standstill, and carry out alcohol precipitation, and centrifugal back gained throw out adopts dehydrated alcohol to clean 2-3 time;
Throw out after g, the dry alcohol that step f is obtained are washed carries out vacuum-drying, pulverizes, and gets the composite edible granulose.
Above-mentioned mushroom, black fungus, glossy ganoderma and Grifola frondosa are done the sporophore raw material, and its ratio of weighing is: mushroom: black fungus: glossy ganoderma: Grifola frondosa=1: 2: 2: 1.
Above-mentioned mushroom, black fungus, glossy ganoderma and Grifola frondosa are done sporophore pulverizing back and are added the distilled water mixing, and mushroom, black fungus, glossy ganoderma and the Grifola frondosa that its distilled water add-on is this pulverizing done 60-80 times of sporophore weight.
Above-mentioned microwave extraction, its microwave power are 300-600W, extraction time 20-40min.
Above-mentioned condensing reflux extracts, and its extraction time is 0.5-1.5h.；
The residue that above-mentioned microwave, condensing reflux extract again adds distilled water and mixes, and the 30-40 that its distilled water add-on is this residue weight doubly.
Add ethanol to determining alcohol during above-mentioned extraction concentrated solution alcohol precipitation and reach 50%-80%, time of repose is 12-24h.
Composite edible granulose by above-mentioned either party's method preparation.
In the raw material of the present invention, mushroom, property is flat, flavor is sweet, nontoxic, has enriching yin, moistening lung, nourishing the stomach, activating blood circulation and supplementing qi, brain tonic effect such as to keep fit.Contained polysaccharide is a kind of nonspecific immunity strengthening agent in the mushroom, has reducing blood-fat, multiple physiologically active, particularly polyglucose such as hypoglycemic have significant anti-cancer activity; Black fungus, property is flat, flavor is sweet, cool blood is arranged, invigorates blood circulation, stops blooding, beneficial stomach, the effect of moisturizing.Polysaccharide in the black fungus is blood fat reducing, cholesterol, blood sugar and lipofuscin content obviously, have effects such as antitumor, anti-inflammatory, anticoagulation, atherosclerosis, anti-hemocyte reduce, anti-radiation, antithrombotic formation, and can strengthen the sOD vigor, reduce the production of objectionable impurities free radical and improve immunizing power; Glossy ganoderma materials for improving vision, tonifying liver gas, calm the nerves, beneficial essence, the beneficial motive, beneficial temper, kidney-nourishing gas, logical nine orifices, ear acute hearing, bright, the sharp joint of order, diuresis, skin maintenance, be nourishing and fit keeping function, consolidate the good merchantable brand of setting upright.A large amount of research reports show: ganoderan is the main component of glossy ganoderma, has neoplasm growth, reduce chemical therapy toxic side effect, tranquilizing and allaying excitement, enhance immunity power, cardiac stimulant, lowering blood pressure, the protection liver, hypoglycemic, anti-oxidant and the anti-ageing effect of waiting for a long time, it is one of glossy ganoderma main effective constituent of strengthening the body resistance to consolidate the constitution; Grifola frondosa is flat, flavor is sweet, be food, the dual-purpose fungi of medicine, nutritious, tasty, in recent years through studies have shown that in a large number: contain grifolan in the Grifola frondosa and have significantly antitumor, AIDS virus resisting, improve function of immune system, blood sugar regulation, blood fat and cholesterol levels hypotensively wait effect, generation and transfer to preventing cancer cells have certain curative effect to hypertension, obesity, diabetes, anemia etc.Therefore, adopt the prepared composite edible granulose physiologically active of above-mentioned raw materials good, effect is comprehensive, stronger anti-chemomorphosis effect is arranged, have antiviral, anti-lipid, regulate immunity system, anti-platelet aggregation, antitumor, protect effects such as liver, anti-transgenation, anti-AIDS, and solvability well easily absorbs.In its preparation method, as extraction agent, adopt microwave extraction and condensing reflux extract phase combining method, the polysaccharide extract rate height with distilled water; Adopt vacuum-drying, avoid the too high physiologically active that influences polysaccharide of temperature.Preparation method's technology of its composite edible granulose is reasonable, workable, easily accomplishes scale production.
Embodiment
The present invention will be further described below in conjunction with embodiment.
Embodiment 1
A kind of preparation method of composite edible granulose, it is to choose mushroom, black fungus, glossy ganoderma and Grifola frondosa to do sporophore be raw material, in mushroom: black fungus: glossy ganoderma: Grifola frondosa=1: 2: 2: 1 the ratio thorough mixing of weighing evenly is placed in the Universalpulverizer and pulverizes, cross 80 mesh sieves, the mushroom, black fungus, glossy ganoderma and the Grifola frondosa that add 60 times of pulverizing are then done the distilled water of sporophore weight, mix to be placed on and carry out microwave extraction in the microwave oven, microwave power is 300W, and extraction time is 40min; After microwave extraction finishes, place the heating jacket of condensing reflux equipment to carry out condensing reflux in the mixture of edible mushrooms mixed powder and water and extract, extraction time is 1.5h; Adopt whizzer with the centrifugal 20min of the rotating speed of 3000r/min, carry out slag, liquid separation, get extracting solution and residue; Microwave extraction and condensing reflux that the distilled water that residue obtained adding residue weight is 40 times carries out once more extract, extracting method and first extract phase with, slag, liquid separate, extracting solution; United extraction liquid, vacuum concentration must extract concentrated solution to 1/3 of original volume; To extract concentrated solution and add ethanol to determining alcohol and reach 50%, thorough mixing evenly after, leave standstill after 24h carries out alcohol precipitation, carry out centrifugally, the gained throw out adopts dehydrated alcohol to clean 2 times, carries out vacuum-drying then, adopt Universalpulverizer to pulverize, 100 orders that sieve get the composite edible granulose.
Composite edible granulose of the present invention can add medically acceptable auxiliary material, is pressed into tablet again.
Embodiment 2
A kind of preparation method of composite edible granulose, it is to choose mushroom, black fungus, glossy ganoderma and Grifola frondosa to do sporophore be raw material, in mushroom: black fungus: glossy ganoderma: Grifola frondosa=1: 2: 2: 1 the ratio thorough mixing of weighing evenly is placed in the Universalpulverizer and pulverizes, cross 80 mesh sieves, the mushroom, black fungus, glossy ganoderma and the Grifola frondosa that add 70 times of pulverizing are then done the distilled water of sporophore weight, mix to be placed on and carry out microwave extraction in the microwave oven, microwave power is 500W, and extraction time is 30min; After microwave extraction finishes, place the heating jacket of condensing reflux equipment to carry out condensing reflux in the mixture of edible mushrooms mixed powder and water and extract, extraction time is 1h; Adopt whizzer with the centrifugal 15min of the rotating speed of 4000r/min, carry out slag, liquid separation, get extracting solution and residue; Microwave extraction and condensing reflux that the distilled water that residue obtained adding residue weight is 35 times carries out once more extract, and extraction and slag, liquid separating method method get extracting solution and residue with identical for the first time; Microwave extraction and condensing reflux that the distilled water that residue obtained adding residue weight is 30 times carries out for the third time extract, and extraction and slag, liquid separating method get extracting solution with identical for the first time; United extraction liquid, vacuum concentration must extract concentrated solution to 1/4 of original volume; To extract concentrated solution and add ethanol to determining alcohol and reach 70%, thorough mixing evenly after, leave standstill after 18h carries out alcohol precipitation, carry out centrifugally, the gained throw out adopts dehydrated alcohol to clean 3 times, carries out vacuum-drying then, adopt Universalpulverizer to pulverize, 100 orders that sieve get the composite edible granulose.
Embodiment 3
A kind of preparation method of composite edible granulose, it is to choose mushroom, black fungus, glossy ganoderma and Grifola frondosa to do sporophore be raw material, in mushroom: black fungus: glossy ganoderma: Grifola frondosa=1: 2: 2: 1 the ratio thorough mixing of weighing evenly is placed in the Universalpulverizer and pulverizes, cross 80 mesh sieves, the mushroom, black fungus, glossy ganoderma and the Grifola frondosa that add 80 times of pulverizing are then done the distilled water of sporophore weight, mix to be placed on and carry out microwave extraction in the microwave oven, microwave power is 600W, and extraction time is 20min; After microwave extraction finishes, place the heating jacket of condensing reflux equipment to carry out condensing reflux in the mixture of edible mushrooms mixed powder and water and extract, extraction time is 0.5h; Adopt filter to carry out slag, liquid separation, get extracting solution and residue; Microwave extraction and condensing reflux that the distilled water that residue obtained adding residue weight is 40 times carries out once more extract, and extraction and slag, liquid separating method method get extracting solution and residue with identical for the first time; Microwave extraction and condensing reflux that the distilled water that residue obtained adding residue weight is 30 times carries out for the third time extract, and extraction and slag, liquid separating method get extracting solution with identical for the first time; United extraction liquid, vacuum concentration must extract concentrated solution to 1/4 of original volume; To extract concentrated solution and add ethanol to determining alcohol and reach 80%, thorough mixing evenly after, leave standstill after 12h carries out alcohol precipitation, carry out centrifugally, the gained throw out adopts dehydrated alcohol to clean 3 times, carries out vacuum-drying then, adopt Universalpulverizer to pulverize, 100 orders that sieve get the composite edible granulose.
Composite edible granulose of the present invention can further be prepared into polyoses grain, the composite edible granulose that is about to preparation adds the dextrin of 2 times of its weight, after thorough mixing is even, adopt 70%-90% ethanol to make softwood as wetting agent, cross 12 orders and shine the net granulation, whole grain, 50 ℃ of-60 ℃ of forced air dryings reach below 5% to moisture content and get final product.
Claims (8)
1. the preparation method of a composite edible granulose is characterized in that: through following technology
The selection of a, raw material is to choose mushroom, black fungus, glossy ganoderma and Grifola frondosa to do sporophore be raw material with handling it, it is weighed in proportion mix the back and pulverize, and adds an amount of distilled water and mixes, and gets the mixture of edible mushrooms powder and water;
The mixture that b, microwave and condensing reflux extract edible mushrooms powder that step a is obtained and water carries out microwave extraction, condensing reflux extraction successively;
Carry out slag, liquid separation after c, slag, liquid separation and Extraction finish, get extracting solution and residue;
D, microwave and condensing reflux extract an amount of distilled water of residue adding that step c is obtained again and mix, and carry out microwave extraction and condensing reflux more successively and extract 1-2 time, respectively carry out slag, liquid separation, get extracting solution and residue;
E, extracting solution concentrate the extracting solution merging that step c and steps d are obtained, and vacuum concentration must extract concentrated solution to the 1/3-1/4 of original volume;
F, alcohol precipitation add ethanol with the extraction concentrated solution that step e obtains, and leave standstill, and carry out alcohol precipitation, and centrifugal back gained throw out adopts dehydrated alcohol to clean 2-3 time;
Throw out after g, the dry alcohol that step f is obtained are washed carries out vacuum-drying, pulverizes, and gets the composite edible granulose.
2. the preparation method of a kind of composite edible granulose according to claim 1, it is characterized in that: described mushroom, black fungus, glossy ganoderma and Grifola frondosa are done the sporophore raw material, and its ratio of weighing is: mushroom: black fungus: glossy ganoderma: Grifola frondosa=1: 2: 2: 1.
3. the preparation method of a kind of composite edible granulose according to claim 1, it is characterized in that: described mushroom, black fungus, glossy ganoderma and Grifola frondosa are done sporophore pulverizing back and are added the distilled water mixing, and mushroom, black fungus, glossy ganoderma and the Grifola frondosa that its distilled water add-on is this pulverizing done 60-80 times of sporophore weight.
4. the preparation method of a kind of composite edible granulose according to claim 1 is characterized in that: described microwave extraction, its microwave power are 300-600W, extraction time 20-40min.
5. the preparation method of a kind of composite edible granulose according to claim 1 is characterized in that: described condensing reflux extracts, and its extraction time is 0.5-1.5h.
6. the preparation method of a kind of composite edible granulose according to claim 1 is characterized in that: the residue that described microwave, condensing reflux extract again adds distilled water and mixes, and the 30-40 that its distilled water add-on is this residue weight doubly.
7. the preparation method of a kind of composite edible granulose according to claim 1 is characterized in that: add ethanol to determining alcohol during described extraction concentrated solution alcohol precipitation and reach 50%-80%, time of repose is 12-24h.
8. by composite edible granulose according to the preparation of claim 1-7 either party method.
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| CN102627789B (en) * | 2012-03-31 | 2014-01-01 | 中华全国供销合作总社济南果品研究院 | Preparation process for edible fungus compound polysaccharide |
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| CN104187678A (en) * | 2014-09-11 | 2014-12-10 | 浙江方格药业有限公司 | Health composition for reducing blood fat and production method of health composition |
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| CN104397531A (en) * | 2014-12-04 | 2015-03-11 | 杨福国 | Gathering and nourishing polysaccharide and preparation process thereof |
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| CN108260808A (en) * | 2018-01-31 | 2018-07-10 | 万宁万维诺丽生物科技有限公司 | A kind of beautiful ferment of promise and preparation method thereof |
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| CN112535287A (en) * | 2020-12-07 | 2021-03-23 | 皖西学院 | Compound edible fungus oral liquid and preparation process thereof |
| CN113951356A (en) * | 2021-10-15 | 2022-01-21 | 上海舒泽生物科技研究所 | Mushroom and ganoderma lucidum polysaccharide composite tablet candy for improving immunity and preparation method thereof |
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