CN102533900A - Method for extracting lentinan - Google Patents
Method for extracting lentinan Download PDFInfo
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- CN102533900A CN102533900A CN2010106046265A CN201010604626A CN102533900A CN 102533900 A CN102533900 A CN 102533900A CN 2010106046265 A CN2010106046265 A CN 2010106046265A CN 201010604626 A CN201010604626 A CN 201010604626A CN 102533900 A CN102533900 A CN 102533900A
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Abstract
The invention discloses a method for extracting lentinan. The method comprises the following steps of: firstly, drying anyone or more of Lentinula edodes sporocarps, Lentinula edodes mycelium and Lentinus edodes stems and then crushing to obtain Lentinula edodes powder; secondly, feeding the obtained Lentinula edodes powder in water of which the amount is 20-25 times of the weight of the Lentinula edodes powder, adding complex enzyme accounting for 0.1 percent of the weight of the Lentinula edodes powder, leaching at the temperature of 75-100DEG C for 2.5-3.5 hours and filtering to obtain an extraction solution; and thirdly, adding complex acid oxidase accounting for 0.05 percent of the weight of the extraction solution and leaching at the temperature of 75-100DEG C for 0.5-1.5 hours; and centrifuging the extraction solution at room temperature or vacuum condensing the extraction solution to obtain lentinan. According to the method disclosed by the invention, the extraction rate for extracting the lentinan from the Lentinus edodes is greatly increased; the utilization rate of raw materials is effectively increased; energy sources are saved; the production cost is reduced; a process path is simple; the equipment input is low; the industrialization is easily realized; and the method is an effective method for comprehensively processing and utilizing the Lentinula edodes.
Description
Technical field
The invention belongs to polysaccharide extractive technique field, be specifically related to a kind of process for extracting of lentinan.
Background technology
Mushroom has another name called fragrant deep, dried mushroom, is one of famous in the world practical fungi.It is not only plain all good and celebrated with color, and nutritious, can cure multiple disease.The traditional Chinese medical science thinks that the mushroom flavor is sweet, property flat, has the effect that air making-up and spleen enlivening and stomach kidney-nourishing, flavour help food.Mushroom not only contains abundant nutrition, and also has special medicinal curative effect.Lentinan is one of main active ingredient of mushroom, has curative effects such as antitumor, antiviral, treatment hepatitis B, is widely used.Japan at first succeeded in developing the lentinan injection in 1985, was used for treating cancer of the stomach.
The present more complicated of process for extracting extraction process of existing lentinan, extraction solvent commonly used is an ethanol, if the ethanol that is used for precipitating lentinan 2-4 times of extracting solution usually, so that polysaccharide is separated out.And the purity of polysaccharide of extracting like this is not high, still need pass through a series of subsequent disposal, has improved cost greatly, makes the price of lentinan higher for a long time.In recent years, have many people that the process for extracting of lentinan is improved, but finally all can't break away from this step of ethanol sedimentation, this extraction step has not only expended a large amount of ethanol, and the lentinan content that obtains is not high.
Summary of the invention
The objective of the invention is to solve the low technical problem of extraction rate that exists in the process for extracting of existing lentinan, a kind of process for extracting of lentinan is provided.
For realizing the foregoing invention purpose, the technical scheme that the present invention adopts is following:
The process for extracting of lentinan of the present invention, this method comprises the steps:
(1) any or multiple crushed after being dried in mushroom fruiting body, shiitake mushroom hypha, the champignon stems is got the mushroom powder;
(2) the above-mentioned mushroom powder that obtains is placed the water of 20 ~ 25 times of weight of mushroom powder, add the prozyme that accounts for mushroom powder weight 1 ‰, 75 ~ 100 ℃ of lixiviates 2.5 ~ 3.5 hours are crossed and are filtered extracting solution;
(3) in the extracting solution that step (2) obtains, add the complex acid oxydase account for extracting solution weight 0.5 ‰, 75 ~ 100 ℃ lixiviate 0.5-1.5 hour; Centrifugal or the vacuum concentration of extracting solution room temperature obtains lentinan.
In the step (1) any or multiple crushed after being dried in mushroom fruiting body, shiitake mushroom hypha, the champignon stems is got the mushroom powder, the mushroom powder size is the 600-900 order.
The described prozyme of step (2) is that mass ratio is the cellulase of 1.5:1 and the prozyme of the plain enzyme of wood sugar.
The pH value of extracting solution is 5.0 in the said step (1).
The pH value of extracting solution is 5.0 in the said step (2).
The inventive method is to improving by extracting the low problem of lentinan traditional method for extracting rate in the champignon stems; In the proposition process, add prozyme and complex acid oxydase; Two steps extracted, and intracellular polyse is fully discharged, thereby obtained the polysaccharide extract rate greater than 16%.The present invention has increased substantially by the extraction yield that extracts lentinan in the mushroom, effectively improves raw material availability, and save energy; Reduce production costs, its operational path is simple and direct, less equipment investment; Being easy to industriallization, is a kind of effective ways of mushroom comprehensive processing and utilization.
Embodiment
Embodiment 1
(1) any or multiple crushed after being dried in mushroom fruiting body, shiitake mushroom hypha, the champignon stems is got the mushroom powder; The mushroom powder size is the 600-900 order.
(2) the above-mentioned mushroom powder that obtains is placed the water of 20 times of weight of mushroom powder, add the prozyme that accounts for mushroom powder weight 1 ‰, the pH value of extracting solution is 5.0,75 ℃ of lixiviates 3.5 hours, and mistake filters extracting solution.Described prozyme is that mass ratio is the cellulase of 1.5:1 and the prozyme of the plain enzyme of wood sugar.
(3) in the extracting solution that step (2) obtains, add the complex acid oxydase that accounts for extracting solution weight 0.5 ‰, the pH value of extracting solution is 5.0,75 ℃ of lixiviates 1.5 hours; Centrifugal or the vacuum concentration of extracting solution room temperature obtains lentinan.The lentinan extraction yield is 16.57%.
Embodiment 2
(1) any or multiple crushed after being dried in mushroom fruiting body, shiitake mushroom hypha, the champignon stems is got the mushroom powder; The mushroom powder size is the 600-900 order.
(2) the above-mentioned mushroom powder that obtains is placed the water of 22 times of weight of mushroom powder, add the prozyme that accounts for mushroom powder weight 1 ‰, the pH value of extracting solution is 5.0,100 ℃ of lixiviates 2.5 hours, and mistake filters extracting solution; The described prozyme of step (2) is that mass ratio is the cellulase of 1.5:1 and the prozyme of the plain enzyme of wood sugar.
(3) in the extracting solution that step (2) obtains, add the complex acid oxydase that accounts for extracting solution weight 0.5 ‰, the pH value of extracting solution is 5.0,100 ℃ of lixiviates 0.5 hour; Centrifugal or the vacuum concentration of extracting solution room temperature obtains lentinan.The lentinan extraction yield is 17.68%.
Embodiment 3
(1) any or multiple crushed after being dried in mushroom fruiting body, shiitake mushroom hypha, the champignon stems is got the mushroom powder; The mushroom powder size is the 600-900 order.
(2) the above-mentioned mushroom powder that obtains is placed the water of 25 times of weight of mushroom powder, add the prozyme that accounts for mushroom powder weight 1 ‰, the pH value of extracting solution is 5.0,80 ℃ of lixiviates 3 hours, and mistake filters extracting solution; The described prozyme of step (2) is that mass ratio is the cellulase of 1.5:1 and the prozyme of the plain enzyme of wood sugar.
(3) in the extracting solution that step (2) obtains, add the complex acid oxydase that accounts for extracting solution weight 0.5 ‰, the pH value of extracting solution is 5.0,80 ℃ of lixiviates 1 hour; Centrifugal or the vacuum concentration of extracting solution room temperature obtains lentinan.The lentinan extraction yield is 19.57%.
Claims (5)
1. the process for extracting of a lentinan is characterized in that this method comprises the steps:
Any or multiple crushed after being dried in mushroom fruiting body, shiitake mushroom hypha, the champignon stems is got the mushroom powder;
The above-mentioned mushroom powder that obtains is placed the water of 20 ~ 25 times of weight of mushroom powder, add the prozyme that accounts for mushroom powder weight 1 ‰, 75 ~ 100 ℃ of lixiviates 2.5 ~ 3.5 hours are crossed and are filtered extracting solution;
In the extracting solution that step (2) obtains, add the complex acid oxydase account for extracting solution weight 0.5 ‰, 75 ~ 100 ℃ lixiviate 0.5-1.5 hour; Centrifugal or the vacuum concentration of extracting solution room temperature obtains lentinan.
2. the process for extracting of lentinan according to claim 1; It is characterized in that: in the step (1) any or multiple crushed after being dried in mushroom fruiting body, shiitake mushroom hypha, the champignon stems is got the mushroom powder, the mushroom powder size is the 600-900 order.
3. the process for extracting of lentinan according to claim 1 and 2 is characterized in that: the described prozyme of step (2) is that mass ratio is the cellulase of 1.5:1 and the prozyme of the plain enzyme of wood sugar.
4. the process for extracting of lentinan according to claim 1 and 2 is characterized in that: the pH value of extracting solution is 5.0 in the said step (1).
5. the process for extracting of lentinan according to claim 4 is characterized in that: the pH value of extracting solution is 5.0 in the said step (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010106046265A CN102533900A (en) | 2010-12-24 | 2010-12-24 | Method for extracting lentinan |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010106046265A CN102533900A (en) | 2010-12-24 | 2010-12-24 | Method for extracting lentinan |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104382929A (en) * | 2014-11-25 | 2015-03-04 | 高秀媛 | Medicine for removing blood garbage and vivotoxin and preparation method thereof |
| CN104829734A (en) * | 2014-10-10 | 2015-08-12 | 沈阳市粮油食品科学研究所 | Method of producing pigment, protein, polysaccharide and dietary fiber from dry lentinula edodes |
| CN107383229A (en) * | 2017-08-18 | 2017-11-24 | 合肥丰洁生物科技有限公司 | A kind of lentinan ultrafiltration extraction process |
| CN112094878A (en) * | 2020-08-18 | 2020-12-18 | 周银根 | Method for extracting lentinan by two-step enzyme, lentinan extract and application thereof |
-
2010
- 2010-12-24 CN CN2010106046265A patent/CN102533900A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104829734A (en) * | 2014-10-10 | 2015-08-12 | 沈阳市粮油食品科学研究所 | Method of producing pigment, protein, polysaccharide and dietary fiber from dry lentinula edodes |
| CN104829734B (en) * | 2014-10-10 | 2017-06-13 | 沈阳市粮油食品科学研究所 | A kind of method of utilization dried thin mushroom production pigment, albumen, polysaccharide and dietary fiber |
| CN104382929A (en) * | 2014-11-25 | 2015-03-04 | 高秀媛 | Medicine for removing blood garbage and vivotoxin and preparation method thereof |
| CN107383229A (en) * | 2017-08-18 | 2017-11-24 | 合肥丰洁生物科技有限公司 | A kind of lentinan ultrafiltration extraction process |
| CN112094878A (en) * | 2020-08-18 | 2020-12-18 | 周银根 | Method for extracting lentinan by two-step enzyme, lentinan extract and application thereof |
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Application publication date: 20120704 |