CN102127043A - Method for extracting and separating pueraria flavonid and pueraria polysaccharide - Google Patents
Method for extracting and separating pueraria flavonid and pueraria polysaccharide Download PDFInfo
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Abstract
The invention relates to a method for extracting and separating pueraria flavonid and pueraria polysaccharide, which comprises the following steps: 1) adding puerariae into ethanol solution to perform primary reflux extraction, filtering to obtain ethanol extract B and secondary filter residue, combining the ethanol extract B and ethanol extract A to obtain pueraria flavonid extract; 2) adding water into the obtained secondary filter residue, performing reflux extraction and filtering to obtain water extract; and 3) concentrating the water extract to obtain water extract concentrate, adding ethanol solution, shaking uniformly, standing, filtering, drying precipitate filtered out to obtain pueraria polysaccharide, mixing the filtrate and the obtained pueraria flavonid extract, concentrating, and drying to obtain pueraria flavonid and recovered ethanol solution. The reagent used in the invention is safe, the operation is convenient, the process is simple, common equipment is used, and the ethanol can be recycled.
Description
Technical field
The present invention relates to a kind of extracting method of natural product, relate in particular to the extraction and the isolating method of a kind of Radix Puerariae flavone and pueraria polysaccharide.
Background technology
The root of kudzu vine another name deer leaves of pulse plants, chicken are neat, are the dry root of elegant jessamine or Pachyrhizua angulatus, are the Chinese medicinal materialss of using always, and book on Chinese herbal medicine is all on the books since the successive dynasties, and sweet, hot, the property of distinguish the flavor of is put down, and have expelling pathogenic factors from muscles for reducing heat, promote the production of body fluid, functions such as promoting eruption, warm positive antidiarrheal, enhance immunity power.At present, the root of kudzu vine is widely used in and improves cardiovascular and cerebrovascular.In the important compound preparations such as treatment diarrhoea, diabetes, consumption is very big, is the important component of preparation.Kind of root of kudzu vine compound preparation surplus including nearly 140 in the Pharmacopoeia of the People's Republic of China and " the Sanitation Ministry medicine standard " wherein mainly contains root of kudzu vine tablet, injection etc.In fact the root of kudzu vine mainly contains two effective constituents: Radix Puerariae total flavones and pueraria polysaccharide.Wherein elegant jessamine to contain Radix Puerariae total flavones more, mainly as the raw material of kudzu vine root preparation, medicine, healthcare products etc., Pachyrhizua angulatus then mainly contains pueraria polysaccharide, is used for making the raw material of root of kudzu vine based food more.Because the effect of flavonoid compound and polysaccharide compound differs greatly, flavonoid compound mainly be have that anticancer antitumor, resisting cardiovascular disease, antianaphylaxis, radioprotective, anti-inflammatory are antibacterial, antiviral, anti-oxidant, physiologically active widely such as anti-mutation and enhancing immunity ability.Can prevent and treat the disease of cardio-cerebrovascular and the disease of respiratory system etc., as treatment coronary heart disease, stenocardia, hypertension etc. are had unusual effect, to preventing and treating myocardial infarction, myocardial ischemia, irregular pulse etc. also play important curative effect.Polysaccharide compound mainly be strengthen immune function of human body, opposing old and feeble, suppress tumour, the anti-bolt of invigorating blood circulation, bring high blood pressure down, blood fat, blood sugar and to the provide protection of main organs such as liver kidney etc.Radix Puerariae flavone is widely used in the industry such as medicine, food, makeup, and low and remarkable unique biological activity because of its toxicity, also be applied to the protective foods aspect, promptly be used as the natural additive of food, makeup, as food dye, sweeting agent, antioxidant etc.Pueraria polysaccharide is because unique physiologically active also enjoys people to favor, pueraria polysaccharide is mainly as food, healthcare products etc., aspect the resistance tumour good curative effect is being arranged, relevant report shows that pueraria polysaccharide has damaging action to the propagation of pheochromocyte oncocyte PC12.
At present, domestic and international research to Radix Puerariae extract is mainly the analysis to this effective constituent of Radix Puerariae flavone, and is less to the research report of pueraria polysaccharide.It is the solvent extraction root of kudzu vine that traditional method generally adopts ethanol, the productive rate of its extract is more than 8%, and the content of flavones is more than 40% in the alcohol extract, and pueraria polysaccharide content is more than 5%, this extract is based on the pharmacological action of flavones, and the immune effect of polysaccharide is not obvious.So expectation has a kind of easy method, allows the main component flavonoid compound of the root of kudzu vine separate with polysaccharide compound always.
Nearest disclosed patent CN101570581A, this patent application document discloses the method for a kind of comprehensively extracting pueraria polysaccharide and flavones: directly use hot water extraction, separate pueraria polysaccharide and flavones with ethanol sedimentation then, because they have only used one step of water extraction, the relatively poor flavones extraction yield of solubility is low in the root of kudzu vine, and the foreign matter content height in the aqueous extract causes separating of Radix Puerariae flavone and polysaccharide comparatively difficult, and the ethanol consumption is big.
Patent CN1493695A, this patent application document disclose a kind of root of kudzu vine simultaneous extraction pueraria starch, pueraria polysaccharide, Radix Puerariae isoflavone technology: utilize enzymolysis, alcohol precipitation, vacuum decompression concentrate, dry to extract pueraria starch, pueraria polysaccharide and Radix Puerariae isoflavone.But adopt the cost of enzymolysis higher, and only with one step of enzymolysis, cause impurity too much, separating of Radix Puerariae flavone and pueraria polysaccharide is difficult, and the ethanol consumption is big.
Summary of the invention
The objective of the invention is to propose for the deficiency that overcomes above-mentioned prior art existence the extraction and the isolating method of a kind of Radix Puerariae flavone and pueraria polysaccharide, it separates simple, and is effective, and ethanol can be recycled.
The present invention solves the problems of the technologies described above the technical scheme that is adopted to be: the extraction of Radix Puerariae flavone and pueraria polysaccharide and isolating method is characterized in that including following steps:
1) gets the root of kudzu vine of certainweight, add mass percent and extract Radix Puerariae flavone for the 50-95% ethanolic soln, wherein the root of kudzu vine (g) is 1 with ethanolic soln (mL) solid-to-liquid ratio: 5-1: 30, carry out refluxing extraction one time, filter, obtain ethanol extract A and a filter residue, one time filter residue adds mass percent 50-95% ethanolic soln again, wherein filter residue (g) is 1 with ethanolic soln (mL) solid-to-liquid ratio: 5-1: 30, carry out secondary back and extract, filter, obtain ethanol extract B and secondary filter residue, ethanol extract B and ethanol extract A are merged, obtain the Radix Puerariae flavone extracting solution;
2) with the secondary filter residue extracting in water pueraria polysaccharide of step 1) gained, wherein secondary filter residue (g) is 1 with water (mL) solid-to-liquid ratio: 5-1: 30, carry out refluxing extraction, and filter, obtain aqueous extract, filter residue abandons;
3) with step 2) aqueous extract of gained is concentrated into the 1/10-1/50 of volume multiple, obtain the water of water extraction concentrated solution and recovery, the water extraction concentrated solution adds mass percent 75-95% ethanolic soln, shakes up, leave standstill 1-12 h, filter the precipitation that leaches, oven dry, obtain pueraria polysaccharide, the Radix Puerariae flavone extracting solution of gained filtrate and step 1) gained merges, and through concentrated, dry, obtains the ethanolic soln of Radix Puerariae flavone and recovery.
Press such scheme, the temperature of the described refluxing extraction of step 1) is 60-90 ℃, and the time is 1-6 h.
Press such scheme, the temperature that the described secondary back of step 1) extracts is 60-90 ℃, and the time is 1-6 h.
Press such scheme, step 2) temperature of described refluxing extraction is 70-100 ℃, the time is 1-6 h.
Press such scheme, the water of the described recovery of step 3) returns step 2 through adding required water) secondary filter residue extracting in water pueraria polysaccharide step, water obtains recycle.
Press such scheme, the ethanolic soln of the described recovery of step 3) is added mass percent 95% ethanol and is modulated into the root of kudzu vine that returns step 1) after the required ethanolic soln concentration and adds ethanolic soln and extract the Radix Puerariae flavone step, and ethanol obtains recycle.
Above-mentioned Radix Puerariae flavone of producing and pueraria polysaccharide can be made tablet, capsule, beverage and oral liquid.
The present invention passes through ethanol extraction method, agents useful for same is nontoxic, easy to operate, technology is simple, used all is general-purpose equipment, ethanol can recycle, the present invention at first adopts ethanolic soln to extract Radix Puerariae flavone, separate Radix Puerariae flavone and pueraria polysaccharide with the two-step approach of water extraction pueraria polysaccharide again, Radix Puerariae flavone extraction yield height not only, and make full use of extraction slag after the alcohol extracting, effectively Radix Puerariae flavone and pueraria polysaccharide are separated, and will concentrate with the pueraria polysaccharide extracting solution of water extraction gained, the concentrated solution ethanol sedimentation separates pueraria polysaccharide and Radix Puerariae flavone, further improve the content of pueraria polysaccharide, in addition, concentrate recovered solvent energy cycling and reutilization, to reduce the discharging of solvent load and waste water.
Description of drawings
Fig. 1 is the extraction and the separation process scheme figure of Radix Puerariae flavone and pueraria polysaccharide.
Embodiment
Embodiment 1
Elegant jessamine 20 g are produced in Yunnan, extract Radix Puerariae flavone with 75% ethanolic soln (mass ratio), pressed the root of kudzu vine (g) and ethanolic soln (mL) solid-to-liquid ratio 1: 20, in the flask of 1 L, 80 ℃ of following refluxing extraction 4 h, filter, obtain ethanol extract A and a filter residue, filter residue use again 75% ethanolic soln (mass ratio) by solid-to-liquid ratio 1: 20 at 80 ℃ of following refluxing extraction 4 h, filter, ethanol extract B that obtains and secondary filter residue merge itself and ethanol extract A, obtain 750 mL Radix Puerariae flavone extracting solutions; With secondary filter residue water extraction pueraria polysaccharide, press filter residue (g) and water (mL) solid-to-liquid ratio 1:20,100 ℃ of following refluxing extraction 4 h filter, and obtain 380 mL aqueous extracts, and filter residue is abandoned it; Aqueous extract is concentrated, obtain 10 mL water extraction concentrated solutions and reclaim the water of 368 mL, the water benefit that reclaims adds water to required volume and is used for secondary filter residue extraction pueraria polysaccharide, water obtains recycle, add 30 mL, 95% ethanolic solns (mass ratio) in the water extraction concentrated solution, shake up, leave standstill 3 h, filter the precipitation that leaches, oven dry obtains 1.71 g pueraria polysaccharides, 35 mL filtrates that obtain and above-mentioned 750 mL Radix Puerariae flavone extracting solutions merge, concentrate, drying, obtaining 2.22 g Radix Puerariae flavone content is 73.25%, the extraction yield of Radix Puerariae flavone is 11.1%, the 700 mL ethanolic solns that concentration and evaporation reclaims are modulated into 75% ethanolic soln (mass ratio) with 150 mL, 95% ethanol (mass ratio), are used for the extraction of Radix Puerariae flavone, and ethanol obtains recycle.
Embodiment 2
Elegant jessamine 20 g are produced in Hubei, extract Radix Puerariae flavone with 80% ethanolic soln (mass ratio), pressed the root of kudzu vine (g) and ethanolic soln (mL) solid-to-liquid ratio 1: 5, in the flask of 500 mL, 80 ℃ of following refluxing extraction 1 h, filter, obtain ethanol extract A and a filter residue, filter residue use again 80% ethanolic soln (mass ratio) by solid-to-liquid ratio 1: 5 at 80 ℃ of following refluxing extraction 4 h, filter, ethanol extract B that obtains and secondary filter residue merge ethanol extract B and ethanol extract A, obtain 190 mL Radix Puerariae flavone extracting solutions; Secondary filter residue water extraction pueraria polysaccharide is pressed filter residue (g) and water (mL) solid-to-liquid ratio 1:5, and 100 ℃ of following refluxing extraction 4 h filter, and obtain 90 mL aqueous extracts, and filter residue is abandoned it; Aqueous extract is concentrated, obtain 3 mL water extraction concentrated solutions and reclaim the water of 86 mL, the water benefit that reclaims adds water to required volume and is used for secondary filter residue extraction pueraria polysaccharide, water obtains recycle, add 15 mL, 95% ethanolic solns (mass ratio) in the water extraction concentrated solution, shake up, leave standstill 3 h, filter the precipitation that leaches, oven dry obtains the 1.58g pueraria polysaccharide, 18mL filtrate that obtains and above-mentioned 190 mL Radix Puerariae flavone extracting solutions merge, concentrate, drying, obtaining 1.85 g Radix Puerariae flavone content is 58.15%, the extraction yield of Radix Puerariae flavone is 9.25%, the 175 mL ethanolic solns that concentration and evaporation reclaims are modulated into 75% ethanolic soln (mass ratio) with 80 mL, 95% ethanol (mass ratio), are used for the extraction of Radix Puerariae flavone, and ethanol obtains recycle.
Embodiment 3
Elegant jessamine 20 g are produced in Zhejiang, extract Radix Puerariae flavone with 75% ethanolic soln (mass ratio), pressed the root of kudzu vine (g) and ethanolic soln (mL) solid-to-liquid ratio 1: 10, in the flask of 500 mL, 80 ℃ of following refluxing extraction 2 h, filter, obtain ethanol extract A and a filter residue, filter residue use again 75% ethanolic soln (mass ratio) by solid-to-liquid ratio 1: 20 at 80 ℃ of following refluxing extraction 2 h, filter, ethanol extract B that obtains and secondary filter residue merge ethanol extract B and ethanol extract A, obtain 380 mL Radix Puerariae flavone extracting solutions; With the water extraction pueraria polysaccharide of the filter residue after the ethanolic soln second extraction, press filter residue (g) and water (mL) solid-to-liquid ratio 1:10,80 ℃ of following refluxing extraction 2 h filter, and obtain 190 mL aqueous extracts, and filter residue is abandoned it; Aqueous extract is concentrated, obtain 7mL water extraction concentrated solution and reclaim the water of 182 mL, the water benefit that reclaims adds water to required volume and is used for secondary filter residue extraction pueraria polysaccharide, water obtains recycle, add 25 mL, 95% ethanolic solns (mass ratio) in the water extraction concentrated solution, shake up, leave standstill 1 h, filter the precipitation that leaches, oven dry obtains 1.67 g pueraria polysaccharides, 27 mL filtrates that obtain and above-mentioned 380 mL Radix Puerariae flavone extracting solutions merge, concentrate, drying, obtaining 2.03 g Radix Puerariae flavone content is 60.17%, the extraction yield of Radix Puerariae flavone is 10.15%, the 400 mL ethanolic solns that concentration and evaporation reclaims are modulated into 75% ethanolic soln (mass ratio) with 210 mL, 95% ethanol (mass ratio), are used for the extraction of Radix Puerariae flavone, and ethanol obtains recycle.
Embodiment 4
Elegant jessamine 50 g are produced in the Hunan, extract Radix Puerariae flavone with 80% ethanolic soln (mass ratio), pressed the root of kudzu vine (g) and ethanolic soln (mL) solid-to-liquid ratio 1: 20, in the flask of 2 L, 80 ℃ of following refluxing extraction 3 h, filter, obtain ethanol extract A and a filter residue, filter residue use again 80% ethanolic soln (mass ratio) by solid-to-liquid ratio 1: 20 at 80 ℃ of following refluxing extraction 3 h, filter, ethanol extract B that obtains and secondary filter residue merge ethanol extract B and ethanol extract A, obtain 1900 mL Radix Puerariae flavone extracting solutions; With ethanolic soln secondary filter residue water extraction pueraria polysaccharide, press filter residue (g) and water (mL) solid-to-liquid ratio 1:20,80 ℃ of following refluxing extraction 3 h filter, and obtain 900 mL aqueous extracts, and filter residue is abandoned it; Aqueous extract is concentrated, obtain 30 mL water extraction concentrated solutions and reclaim the water of 864 mL, the water benefit that reclaims adds water to required volume and is used for secondary filter residue extraction pueraria polysaccharide, water obtains recycle, add 75 mL, 95% ethanolic solns (mass ratio) in the water extraction concentrated solution, shake up, leave standstill 3 h, filter the precipitation that leaches, oven dry obtains 4.52 g pueraria polysaccharides, 90mL filtrate that obtains and above-mentioned 1900 mL Radix Puerariae flavone extracting solutions merge, concentrate, drying, obtaining 5.53 g Radix Puerariae flavone content is 70.55%, the extraction yield of Radix Puerariae flavone is 11.06%, the 1980 mL ethanolic solns that concentration and evaporation reclaims are modulated into 75% ethanolic soln (mass ratio) with 905 mL, 95% ethanol (mass ratio), are used for the extraction of Radix Puerariae flavone, and ethanol obtains recycle.
Each cited raw material of the present invention can both be realized the present invention, and the bound value of each raw material, interval value can both realize the present invention; Do not enumerate embodiment one by one at this.Bound value, the interval value of processing parameter of the present invention (as temperature, time etc.) can both be realized the present invention, do not enumerate embodiment one by one at this.
Claims (6)
1. the extraction and the isolating method of Radix Puerariae flavone and pueraria polysaccharide is characterized in that including following steps:
1) gets the root of kudzu vine of certainweight, add mass percent and extract Radix Puerariae flavone for the 50-95% ethanolic soln, wherein the root of kudzu vine (g) is 1 with ethanolic soln (mL) solid-to-liquid ratio: 5-1: 30, carry out refluxing extraction one time, filter, obtain ethanol extract A and a filter residue, one time filter residue adds mass percent 50-95% ethanolic soln again, wherein filter residue (g) is 1 with ethanolic soln (mL) solid-to-liquid ratio: 5-1: 30, carry out secondary back and extract, filter, obtain ethanol extract B and secondary filter residue, ethanol extract B and ethanol extract A are merged, obtain the Radix Puerariae flavone extracting solution;
2) with the secondary filter residue extracting in water pueraria polysaccharide of step 1) gained, wherein secondary filter residue (g) is 1 with water (mL) solid-to-liquid ratio: 5-1: 30, carry out refluxing extraction, and filter, obtain aqueous extract, filter residue abandons;
3) with step 2) aqueous extract of gained is concentrated into the 1/10-1/50 of volume multiple, obtain the water of water extraction concentrated solution and recovery, the water extraction concentrated solution adds mass percent 75-95% ethanolic soln, shakes up, leave standstill 1-12 h, filter the precipitation that leaches, oven dry, obtain pueraria polysaccharide, the Radix Puerariae flavone extracting solution of gained filtrate and step 1) gained merges, and through concentrated, dry, obtains the ethanolic soln of Radix Puerariae flavone and recovery.
2. by the extraction and the isolating method of described Radix Puerariae flavone of claim 1 and pueraria polysaccharide, the temperature that it is characterized in that the described refluxing extraction of step 1) is 60-90 ℃, and the time is 1-6 h.
3. by the extraction and the isolating method of claim 1 or 2 described Radix Puerariae flavones and pueraria polysaccharide, it is characterized in that the temperature that the described secondary back of step 1) extracts is 60-90 ℃, the time is 1-6 h.
4. by the extraction and the isolating method of the described Radix Puerariae flavone of claim 3 and pueraria polysaccharide, it is characterized in that step 2) temperature of described refluxing extraction is 70-100 ℃, the time is 1-6 h.
5. press the extraction and the isolating method of claim 1 or 2 described Radix Puerariae flavones and pueraria polysaccharide, the water that it is characterized in that the described recovery of step 3) returns step 2 through adding required water) secondary filter residue extracting in water pueraria polysaccharide step, water obtains recycle.
6. press the extraction and the isolating method of claim 1 or 2 described Radix Puerariae flavones and pueraria polysaccharide, the ethanolic soln that it is characterized in that the described recovery of step 3) is added mass percent 95% ethanol and is modulated into the root of kudzu vine that returns step 1) after the required ethanolic soln concentration and adds ethanolic soln and extract the Radix Puerariae flavone step, and ethanol obtains recycle.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103859391A (en) * | 2012-12-11 | 2014-06-18 | 镇江市丹徒区南山溪园茶叶专业合作社 | Preparation method of natural plant flavone mixed capsules |
| CN107880148A (en) * | 2017-12-29 | 2018-04-06 | 广东省肇庆市质量计量监督检测所 | A kind of extracting method of Pachyrhizua angulatus polysaccharide |
| CN109078043A (en) * | 2018-09-27 | 2018-12-25 | 广东环凯微生物科技有限公司 | A kind of pueraria root health care product and preparation method thereof increasing bone density |
| CN109180829A (en) * | 2018-08-27 | 2019-01-11 | 杨鑫 | A kind of preparation method and application of Pueraria Flavonid and polyoses extract |
| CN111171172A (en) * | 2020-02-19 | 2020-05-19 | 四川健腾生物技术有限公司 | Preparation method of pueraria polysaccharide extract |
| CN111528473A (en) * | 2020-04-24 | 2020-08-14 | 浙江师范大学行知学院 | Method for preparing radix puerariae aqueous extract by utilizing natural selenium-rich radix puerariae residues |
| CN111990657A (en) * | 2020-07-14 | 2020-11-27 | 浙江师范大学行知学院 | Process for preparing functional food by utilizing natural selenium-rich radix puerariae residues |
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| CN1872081A (en) * | 2005-06-03 | 2006-12-06 | 天津天士力制药股份有限公司 | Application of kudzuvine root flavone in preparing medicine for treating chronic insufficiency of blood supply for brain |
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Patent Citations (1)
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| CN1872081A (en) * | 2005-06-03 | 2006-12-06 | 天津天士力制药股份有限公司 | Application of kudzuvine root flavone in preparing medicine for treating chronic insufficiency of blood supply for brain |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103859391A (en) * | 2012-12-11 | 2014-06-18 | 镇江市丹徒区南山溪园茶叶专业合作社 | Preparation method of natural plant flavone mixed capsules |
| CN107880148A (en) * | 2017-12-29 | 2018-04-06 | 广东省肇庆市质量计量监督检测所 | A kind of extracting method of Pachyrhizua angulatus polysaccharide |
| CN109180829A (en) * | 2018-08-27 | 2019-01-11 | 杨鑫 | A kind of preparation method and application of Pueraria Flavonid and polyoses extract |
| CN109078043A (en) * | 2018-09-27 | 2018-12-25 | 广东环凯微生物科技有限公司 | A kind of pueraria root health care product and preparation method thereof increasing bone density |
| CN111171172A (en) * | 2020-02-19 | 2020-05-19 | 四川健腾生物技术有限公司 | Preparation method of pueraria polysaccharide extract |
| CN111528473A (en) * | 2020-04-24 | 2020-08-14 | 浙江师范大学行知学院 | Method for preparing radix puerariae aqueous extract by utilizing natural selenium-rich radix puerariae residues |
| CN111990657A (en) * | 2020-07-14 | 2020-11-27 | 浙江师范大学行知学院 | Process for preparing functional food by utilizing natural selenium-rich radix puerariae residues |
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Application publication date: 20110720 |