CN109160955A - The method of dragon fruit pericarp extraction polysaccharide - Google Patents
The method of dragon fruit pericarp extraction polysaccharide Download PDFInfo
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- CN109160955A CN109160955A CN201811309376.5A CN201811309376A CN109160955A CN 109160955 A CN109160955 A CN 109160955A CN 201811309376 A CN201811309376 A CN 201811309376A CN 109160955 A CN109160955 A CN 109160955A
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- dragon fruit
- polysaccharide
- ultrasonic
- fruit pericarp
- leaching
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
Abstract
The method that the invention discloses a kind of to extract polysaccharide from dragon fruit pericarp, it is specifically related to the method using polysaccharide in ultrasonic wave extraction dragon fruit pericarp, and specific steps include: feedstock processing, ultrasonic-leaching, ultrafiltration membrane retention concentration, freeze-drying.The present invention substantially increases the recovery rate of polysaccharide in dragon fruit pericarp using ultrasonic-leaching method, while making dragon fruit pericarp polysaccharide pollution-free during the separation process using membrane separation technique, and no solvent residue and phase transformation generate；The extracting method is easy to operate, is convenient for control and dragon fruit Polyose extraction efficiency with higher.The invention combines ultrasonic wave extraction and membrane separation technique, so that being free of any organic solvent in the dragon fruit pericarp polysaccharide extracted, and the physiological activity of polysaccharide in dragon fruit pericarp is remained to greatest extent, product quality is preferable, can be widely applied to food processing field and field of medicaments.
Description
Technical field
The invention belongs to the deep processing extraction processes of agricultural product dragon fruit pericarp polysaccharide, and in particular to be a kind of from flue
The method that polysaccharide is extracted in fruit pericarp, belongs to deep-processing technical field of agricultural products.
Background technique
Dragon fruit (Hylocereusundatus) is Cactaeous plants, and dragon fruit has fabulous ornamental value, opening
Colored and fruiting period has unique scene, and flue fruits are in olive-shaped, outer volume of the appearance with imperial squama shape, so it is claimed
Make dragon fruit, also known as there is pitaya fruit etc..Dragon fruit is a kind of tool rich in nutriments such as sugar, organic acid, protein, vitamins
The tropical fruit (tree) for thering is highly nourishing health care to be worth.Therefore, source of the dragon fruit as functional component, related disease may be prevented by providing
Nutrient, be conducive to improve consumer physical and mental health.Contain a large amount of plant polyoses in dragon fruit pericarp, studies have shown that planting
Object polysaccharide have multiple biological activities, such as it is anti-oxidant, adjusting it is immune, antitumor, hypoglycemic, antibacterial.In addition, some plants are more
Sugar also has antibacterial activity.Ultrasonic wave assisted extraction is a kind of common method that effective component is extracted from plants, it utilizes super
" cavitation " and second order effect that sound wave generates, such as mechanical oscillation, emulsification, spread, smash, chemical effect, acceleration is intended to mention
The dispersal events of ingredient are taken, to greatly shorten extraction time, improve extraction efficiency, it is effective to be widely used in plant for it in recent years
The extraction of ingredient is studied.
Summary of the invention
The purpose of the present invention is extracting the low deficiency of polysaccharide yield for Polyose extraction technology in existing dragon fruit pericarp, mention
A kind of method for having supplied efficiently extracting polysaccharide from dragon fruit pericarp.This method uses ultrasonic wave extraction combination membrane separation process,
Entire extraction process is easy to operate, and extraction efficiency is high.
The present invention solves its technical problem and adopts the following technical solutions to achieve:
The method of polysaccharide is extracted in dragon fruit pericarp, comprising the following steps: feedstock processing → ultrasonic-leaching → ultrafiltration membrane
Retain concentration → freeze-drying → dragon fruit pericarp polysaccharide.
Specifically includes the following steps:
S1: feedstock processing: dragon fruit pericarp is sliced after cleaning, and is dried, then pulverize and sieve in drying box, is obtained dragon fruit fruit
Pi Fen, as next step raw material；
S2: ultrasonic-leaching: dragon fruit fruit peel powder is added water to submerge, and ultrasonic wave is recycled to extract processing, extracts knot
It is centrifuged after beam, the liquid after collecting centrifugal treating obtains extracting solution；
S3: ultrafiltration membrane retention concentration: using Ultra filtration membrane technical treatment extracting solution, completes point of dragon fruit pericarp polysaccharide
From, then be concentrated under reduced pressure, obtain sediment.
S4: freeze-drying: above-mentioned sediment is first freeze-dried with after organic solvent washing again, crushed after being dried obtains
To dragon fruit pericarp polysaccharide.
Preferentially, in step S1, the drying is dried in drying box under the conditions of 50-60 DEG C, and sieving is to use 80-100
The polished standard screen sieving.
Preferential, in step S2, the process conditions of the ultrasonic-leaching are:
(1) solid-liquid ratio of dragon fruit fruit peel powder and water is 1g:30mL~40mL；
(2) Extracting temperature is 60~70 DEG C；
(3) ultrasonic-leaching uses 200~300W of power；
(4) the ultrasonic-leaching time is 30~40min, and extraction time is 3-5 times；
(5) centrifugal speed is 6000~7000rpm, 15~30min of centrifugation time.
Preferentially, the optimum process condition of the ultrasonic-leaching is:
(1) solid-liquid ratio of dragon fruit fruit peel powder and water is 1g:40mL；
(2) Extracting temperature is 66 DEG C；
(3) ultrasonic-leaching uses power 280W；
(4) the ultrasonic-leaching time is 40min, and extraction time is 5 times；
(5) centrifugal speed is 7000rpm, centrifugation time 30min.
Preferentially, in step S3, the concrete technology of the ultrafiltration membrane retention concentration is: using ultrafiltration membrane (10000-
15000D) the step S2 extracting solution prepared is separated, leaching liquor in collection membrane, leaching liquor carries out under conditions of 4-10 DEG C
It is concentrated under reduced pressure.
Preferentially, in step S4, the organic solvent is that absolute alcohol, ether, acetone are both any or any
Mixture or three's mixture.
The advantages and positive effects of the present invention are:
(1) invention substantially increases the recovery rate of dragon fruit pericarp polysaccharide using ultrasonic-leaching method, while using film point
Make dragon fruit pericarp polysaccharide pollution-free during the separation process from technology, no solvent residue and phase transformation generate；
(2) extracting method it is easy to operate, convenient for control and dragon fruit pericarp polysaccharide with higher recovery rate, should
Invention combines ultrasonic wave extraction and membrane separation technique, so that without any in the dragon fruit pericarp polysaccharide extracted
Organic solvent, and the physiological activity of polysaccharide in dragon fruit pericarp is remained to greatest extent, product quality is preferable, can be extensive
For food processing field and field of medicaments；
(3) active constituent rich in dragon fruit pericarp, which is used, and both can be reduced it to environment
Pollution, and the economic value of dragon fruit pericarp can be improved again.
Specific embodiment
The embodiment of the present invention is described in further detail below, it is emphasized that, embodiment of the present invention is explanation
Property, without being restrictive, therefore the present invention is not limited to the embodiments described in specific embodiment, all by this field
The other embodiment that technical staff obtains according to the technique and scheme of the present invention, also belongs to the scope of protection of the invention.
Technical process of the invention is as follows:
S1: feedstock processing: dragon fruit pericarp is sliced after cleaning, and is dried, then pulverize and sieve in drying box, is obtained dragon fruit fruit
Pi Fen, as next step raw material；
S2: ultrasonic-leaching: dragon fruit fruit peel powder is added water to submerge, and ultrasonic wave is recycled to extract processing, extracts knot
It is centrifuged after beam, the liquid after collecting centrifugal treating obtains extracting solution；
S3: ultrafiltration membrane retention concentration: using Ultra filtration membrane technical treatment extracting solution, completes point of dragon fruit pericarp polysaccharide
From, then be concentrated under reduced pressure, obtain sediment.
S4: it freeze-drying: is freeze-dried again after above-mentioned sediment is first washed with dehydrated alcohol, crushed after being dried obtains
To dragon fruit pericarp polysaccharide.
Embodiment 1
(1) feedstock processing: taking 1kg dragon fruit pericarp, be sliced after being cleaned, be placed on drying box, is 50 DEG C in temperature
Under the conditions of dry, take out the pitaya peel of drying, then be mechanically pulverized, with 80 the polished standard screens be sieved, collect sieving after
Powder obtains dragon fruit fruit peel powder；
(2) ultrasonic-leaching: dragon fruit fruit peel powder is mixed for 1g:30mL with water according to solid-liquid ratio and is poured into container, is shaken
It is placed after even and extracts processing 30min in ultrasonic instrument, ultrasonic power 200W, temperature is 60 DEG C, and repetitive operation is super
Sound wave extract 3 times, then by its centrifugal speed be 6000rpm, centrifugation time 15min, collect centrifugal treating after liquid, must mention
Take liquid；
(3) ultrafiltration membrane retention concentration: the extracting solution of above-mentioned preparation is separated with ultrafiltration membrane (10000), extraction in collection membrane
Liquid, leaching liquor are concentrated under reduced pressure under conditions of 4 DEG C, obtain sediment；
(4) it is freeze-dried: being freeze-dried again after above-mentioned sediment is first washed with absolute alcohol, crushed after being dried obtains
To dragon fruit pericarp polysaccharide.
Embodiment 2
(1) feedstock processing: taking 1kg dragon fruit pericarp, be sliced after being cleaned, be placed on drying box, is 60 DEG C in temperature
Under the conditions of dry, take out the pitaya peel of drying, then be mechanically pulverized, with 100 the polished standard screens be sieved, collect sieving after
Powder obtains dragon fruit fruit peel powder；
(2) ultrasonic-leaching: dragon fruit fruit peel powder is mixed for 1g:40mL with water according to solid-liquid ratio and is poured into container, is shaken
It is placed after even and extracts processing 40min in ultrasonic instrument, ultrasonic power 300W, temperature is 70 DEG C, and repetitive operation is super
Sound wave extract 5 times, then by its centrifugal speed be 7000rpm, centrifugation time 30min, collect centrifugal treating after liquid, must mention
Take liquid；
(3) ultrafiltration membrane retention concentration: the extracting solution of above-mentioned preparation is separated with ultrafiltration membrane (15000D), extraction in collection membrane
Liquid, leaching liquor are concentrated under reduced pressure under conditions of 10 DEG C, obtain sediment；
(4) it is freeze-dried: being freeze-dried again after above-mentioned sediment is first washed with ether, crushed after being dried obtains fire
Imperial fruit pericarp polysaccharide.
Embodiment 3
(1) feedstock processing: taking 2kg dragon fruit pericarp, be sliced after being cleaned, be placed on drying box, is 55 DEG C in temperature
Under the conditions of dry, take out the pitaya peel of drying, then be mechanically pulverized, with 90 the polished standard screens be sieved, collect sieving after
Powder obtains dragon fruit fruit peel powder；
(2) ultrasonic-leaching: dragon fruit fruit peel powder is mixed for 1g:35mL with water according to solid-liquid ratio and is poured into container, is shaken
It is placed after even and extracts processing 35min in ultrasonic instrument, ultrasonic power 250W, temperature is 65 DEG C, and repetitive operation is super
Sound wave extract 4 times, then by its centrifugal speed be 6500rpm, centrifugation time 20min, collect centrifugal treating after liquid, must mention
Take liquid；
(3) ultrafiltration membrane retention concentration: the extracting solution of above-mentioned preparation is separated with ultrafiltration membrane (12000D), extraction in collection membrane
Liquid, leaching liquor are concentrated under reduced pressure under conditions of 8 DEG C, obtain sediment；
(4) it is freeze-dried: above-mentioned sediment being first freeze-dried with after acetone washing again, crushed after being dried obtains fire
Imperial fruit pericarp polysaccharide.
Embodiment 4
(1) feedstock processing: taking 2kg dragon fruit pericarp, be sliced after being cleaned, be placed on drying box, is 60 DEG C in temperature
Under the conditions of dry, take out the pitaya peel of drying, then be mechanically pulverized, with 90 the polished standard screens be sieved, collect sieving after
Powder obtains dragon fruit fruit peel powder；
(2) ultrasonic-leaching: dragon fruit fruit peel powder is mixed for 1g:32mL with water according to solid-liquid ratio and is poured into container, is shaken
It is placed after even and extracts processing 32min in ultrasonic instrument, ultrasonic power 220W, temperature is 62 DEG C, and repetitive operation is super
Sound wave extract 4 times, then by its centrifugal speed be 6800rpm, centrifugation time 25min, collect centrifugal treating after liquid, must mention
Take liquid；
(3) ultrafiltration membrane retention concentration: the extracting solution of above-mentioned preparation is separated with ultrafiltration membrane (12000D), extraction in collection membrane
Liquid, leaching liquor are concentrated under reduced pressure under conditions of 5 DEG C, obtain sediment；
(4) it is freeze-dried: it is dry to carry out freezing after above-mentioned sediment is first washed with both absolute alcohol, ether mixture again
Dry, crushed after being dried obtains dragon fruit pericarp polysaccharide.
Embodiment 5
(1) feedstock processing: taking 2kg dragon fruit pericarp, be sliced after being cleaned, be placed on drying box, is 57 DEG C in temperature
Under the conditions of dry, take out the pitaya peel of drying, then be mechanically pulverized, with 100 the polished standard screens be sieved, collect sieving after
Powder obtains dragon fruit fruit peel powder；
(2) ultrasonic-leaching: dragon fruit fruit peel powder is mixed for 1g:40mL with water according to solid-liquid ratio and is poured into container, is shaken
It is placed after even and extracts processing 40min in ultrasonic instrument, ultrasonic power 280W, temperature is 66 DEG C, and repetitive operation is super
Sound wave extract 5 times, then by its centrifugal speed be 7000rpm, centrifugation time 30min, collect centrifugal treating after liquid, must mention
Take liquid；
(3) ultrafiltration membrane retention concentration: the extracting solution of above-mentioned preparation is separated with ultrafiltration membrane (10000D), extraction in collection membrane
Liquid, leaching liquor are concentrated under reduced pressure under conditions of 9 DEG C, obtain sediment；
(4) it is freeze-dried: being carried out again after above-mentioned sediment is first washed with absolute alcohol, ether, acetone three's mixture
Freeze-drying, crushed after being dried obtain dragon fruit pericarp polysaccharide.
Comparative example 1:
Step the difference from the implementation 1 is that, step ultrasonic-leaching be changed to conventional heating extraction, conventional heating extract temperature
Degree is 60 DEG C, and conventional heating extraction time is 30min, and repetitive operation heating extracts 3 times.
Comparative example 2:
Step and implement 2 the difference is that, step ultrasonic-leaching be changed to conventional heating extraction, conventional heating extract temperature
Degree is 70 DEG C, and conventional heating extraction time is 40min, and repetitive operation heating extracts 5 times.
Comparative example 3:
Step and implement 3 the difference is that, step ultrasonic-leaching be changed to conventional heating extraction, conventional heating extract temperature
Degree is 65 DEG C, and conventional heating extraction time is 35min, and repetitive operation heating extracts 4 times.
Comparative example 4:
Step and implement 4 the difference is that, step ultrasonic-leaching be changed to conventional heating extraction, conventional heating extract temperature
Degree is 62 DEG C, and conventional heating extraction time is 32min, and repetitive operation heating extracts 4 times.
Comparative example 5:
Step and implement 5 the difference is that, step ultrasonic-leaching be changed to conventional heating extraction, conventional heating extract temperature
Degree is 66 DEG C, and conventional heating extraction time is 40min, and repetitive operation heating extracts 5 times.
By the process conditions of the ultrasonic-leaching in embodiment 1-5, (solid-liquid ratio, ultrasonic wave extraction handle time, ultrasonic wave
Temperature when power, ultrasonic-leaching) and polysaccharide extract rate be included in table 1, it is special that comparative example 1-5 is normal in order to evaluate technique
The data one for advising heating extraction (solid-liquid ratio, conventional heating extraction time, conventional heating Extracting temperature) and polysaccharide extract rate are arranged side by side
Enter table 1.
Table 1: embodiment and comparative example scheme and data result
By table 1, analyze obtaining known to sugared rate data for embodiment 1-5: embodiment 5 obtains sugared rate highest, ultrasonic-leaching
Optimum process condition be: solid-liquid ratio 1g:40mL, ultrasonic wave extraction handle 40min, ultrasonic power 280W, and temperature is
66 DEG C, repetitive operation ultrasonic-leaching 5 times.Known to the sugared rate data for obtaining sugared rate and comparative example 1-5 of analysis embodiment 1-5: adopting
With ultrasonic wave extraction handle than using conventional heating extract, finally sugared rate significantly improves, during dragon fruit Polyose extraction,
Using ultrasonic wave step, it can be achieved that efficient Polyose extraction.
Claims (7)
1. extracting the method for polysaccharide in dragon fruit pericarp, it is characterised in that method includes the following steps:
S1: feedstock processing: dragon fruit pericarp is sliced after cleaning, and is dried, then pulverize and sieve in drying box, is obtained dragon fruit pericarp
Powder, as next step raw material；
S2: ultrasonic-leaching: dragon fruit fruit peel powder is added water to submerge, and recycles ultrasonic wave to extract processing, after extraction
Centrifugation, the liquid after collecting centrifugal treating, obtains extracting solution；
S3: ultrafiltration membrane retention concentration: using Ultra filtration membrane technical treatment extracting solution, complete the separation of dragon fruit pericarp polysaccharide,
It is concentrated under reduced pressure again, obtains sediment；
S4: freeze-drying: above-mentioned sediment is first freeze-dried with after organic solvent washing again, crushed after being dried obtains fire
Imperial fruit pericarp polysaccharide.
2. the method for polysaccharide is extracted in dragon fruit pericarp according to claim 1, which is characterized in that in step sl, described
Drying is dried in drying box under the conditions of 50-60 DEG C.
3. the method for polysaccharide is extracted in dragon fruit pericarp according to claim 1, which is characterized in that in step sl, described
Sieving is sieved with 80-100 the polished standard screen.
4. the method for polysaccharide is extracted in dragon fruit pericarp according to claim 1, which is characterized in that in step s 2, described
The process conditions of ultrasonic-leaching are:
(1) solid-liquid ratio of dragon fruit fruit peel powder and water is 1g:30mL~40mL；
(2) Extracting temperature is 60~70 DEG C；
(3) ultrasonic-leaching uses 200~300W of power；
(4) the ultrasonic-leaching time is 30~40min, and extraction time is 3-5 times；
(5) centrifugal speed is 6000~7000rpm, 15~30min of centrifugation time.
5. the method for polysaccharide is extracted in dragon fruit pericarp according to claim 1, which is characterized in that in step s3, described
The concrete technology of ultrafiltration membrane retention concentration is: being divided the step S2 extracting solution prepared using ultrafiltration membrane (10000-15000D)
From leaching liquor in collection membrane, leaching liquor is concentrated under reduced pressure under conditions of 4-10 DEG C.
6. the method for polysaccharide is extracted in dragon fruit pericarp according to claim 1, which is characterized in that in step s 4, described
Organic solvent is absolute alcohol, ether, acetone both any or any mixture or three's mixture.
7. the method for polysaccharide is extracted in -6 any dragon fruit pericarps according to claim 1, which is characterized in that the ultrasonic wave
The process conditions of extraction are:
(1) solid-liquid ratio of dragon fruit fruit peel powder and water is 1g:40mL；
(2) Extracting temperature is 66 DEG C；
(3) ultrasonic-leaching uses power 280W；
(4) the ultrasonic-leaching time is 40min, and extraction time is 5 times；
(5) centrifugal speed is 7000rpm, centrifugation time 30min.
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| CN201811309376.5A CN109160955A (en) | 2018-11-05 | 2018-11-05 | The method of dragon fruit pericarp extraction polysaccharide |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113321751A (en) * | 2021-07-16 | 2021-08-31 | 电子科技大学中山学院 | Method for extracting red-heart pitaya peel polysaccharide |
| CN114478817A (en) * | 2022-02-24 | 2022-05-13 | 华南理工大学 | Pitaya peel polysaccharide and preparation method and application thereof |
| CN114680085A (en) * | 2020-12-29 | 2022-07-01 | 肥西吉新蚕桑专业合作社 | Method for cultivating colored silkworms in greenhouse |
| CN115057949A (en) * | 2022-07-22 | 2022-09-16 | 广西壮族自治区农业科学院 | Active polysaccharide compound extracted from dragon fruit stem and preparation method and application thereof |
| NL2029555B1 (en) * | 2021-08-27 | 2023-03-15 | Guangxi Academy Agricultural Sciences | Method for simultaneously extracting phytosterols and polysaccharides from pitaya stems |
-
2018
- 2018-11-05 CN CN201811309376.5A patent/CN109160955A/en active Pending
Non-Patent Citations (2)
| Title |
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| 但德苗等: "超声波辅助法提取火龙果果皮多糖工艺优化及其抗氧化活性研究", 《农产品加工》 * |
| 李云飞等: "《食品工程原理（第3版）》", 31 August 2014, 中国农业大学出版社 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114680085A (en) * | 2020-12-29 | 2022-07-01 | 肥西吉新蚕桑专业合作社 | Method for cultivating colored silkworms in greenhouse |
| CN113321751A (en) * | 2021-07-16 | 2021-08-31 | 电子科技大学中山学院 | Method for extracting red-heart pitaya peel polysaccharide |
| NL2029555B1 (en) * | 2021-08-27 | 2023-03-15 | Guangxi Academy Agricultural Sciences | Method for simultaneously extracting phytosterols and polysaccharides from pitaya stems |
| CN114478817A (en) * | 2022-02-24 | 2022-05-13 | 华南理工大学 | Pitaya peel polysaccharide and preparation method and application thereof |
| CN115057949A (en) * | 2022-07-22 | 2022-09-16 | 广西壮族自治区农业科学院 | Active polysaccharide compound extracted from dragon fruit stem and preparation method and application thereof |
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