CN106720377A - A kind of preparation method of ginsenoside LBP-X Yoghourt chewable tablets - Google Patents
A kind of preparation method of ginsenoside LBP-X Yoghourt chewable tablets Download PDFInfo
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- CN106720377A CN106720377A CN201611099429.6A CN201611099429A CN106720377A CN 106720377 A CN106720377 A CN 106720377A CN 201611099429 A CN201611099429 A CN 201611099429A CN 106720377 A CN106720377 A CN 106720377A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/18—Milk in dried and compressed or semi-solid form
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
- A23C9/1307—Milk products or derivatives; Fruit or vegetable juices; Sugars, sugar alcohols, sweeteners; Oligosaccharides; Organic acids or salts thereof or acidifying agents; Flavours, dyes or pigments; Inert or aerosol gases; Carbonation methods
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C2240/00—Use or particular additives or ingredients
- A23C2240/15—Use of plant extracts, including purified and isolated derivatives thereof, as ingredient in dairy products
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- Chemical & Material Sciences (AREA)
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- Medicines Containing Plant Substances (AREA)
Abstract
A kind of preparation method of ginsenoside LBP-X Yoghourt chewable tablets of the present invention, belongs to the field of foods processing technique.The preparation process of product of the present invention：First, ginsenoside is extracted.2nd, LBP-X is extracted.3rd, the preparation of Yoghourt chewable tablets.Specifically with ginseng, matrimony vine as raw material is cleaned, drying, broken, ultrasonic assistant is extracted, freeze-drying obtains ginsenoside and LBP-X.The ginsenoside and LBP-X of extraction are added to Yoghourt chewable tablets is made in Yoghourt, two kinds of material nutrient components is brought out the best in each other, so as to develop a kind of new product --- ginsenoside LBP-X Yoghourt chewable tablets.This product not only rolls into one the functional characteristic of ginsenoside and LBP-X, but also is a nutrition, easily health care, product.
Description
Technical field：
The invention belongs to food processing technology field, more particularly to a kind of ginsenoside LBP-X Yoghourt chewable tablets its
Preparation method.
Background technology：
It is various with anti-grease matter mistake containing ginsenoside, ginseng polyacetylene class compound and PDS etc. in ginseng
The material of the effect of oxidation, the antioxidant and anti-aging function of ginseng derives from this mostly.Ginsenoside has many physiological functions
For example：Antitumor, antifatigue, anti-oxidant, reduction blood sugar, enhancing organism adaptation, raising sexual function, protection bone, regulation are refreshing
Through cental system, strengthen immunity etc..
Matrimony vine refers to the ripening fruits of matrimony vine of solanaceae plant, and it has various functional characteristics, and the nourishing liver and kidney, strengthening the essence of energy is bright
Mesh, for consumption consumptive loss, soreness of waist and knee joint, dizziness and tinnitus, Heat Diabetes, blood deficiency chlorosis, blurred vision, is ratified by the Ministry of Public Health
Integration of drinking and medicinal herbs food, proper amount of edible is good for health.LBP-X is one of main active in matrimony vine, and it mainly has
Have and adjust the immune, function of anti-aging, can be with prevention of tumor and AIDS.In addition to this it is possible to be effectively improved old age
People's fatiguability, poor appetite and eye-blurred symptom and with reducing blood lipid, AFL, antitumor, the anti-ageing effect of waiting for a long time.
Yoghourt chewable tablets has the advantages that directly to eat on edibility characteristic, and long-term taking is to the people with enterogastritis
There is very great benefit.Although process equipment is more aborning for Yoghourt chewable tablets, product function comprehensively, nutritive value compared with
It is high.
Current ginseng food has a ginseng pulverate, ginseng oral liquid, ginseng lozenge, but ginseng purity in these products is very
Height, is only suitable for elderly and infirm and eats.Prepared by Yoghourt chewable tablets hardness it is suitable, the submissive exquisiteness of entrance, form is complete, surface
It is smooth, comfortable acid, with strong ginseng matrimony vine local flavor and Yoghourt fragrance, and ginseng, matrimony vine content are moderate, are adapted to all kinds of people
Group is edible.
The content of the invention：
The technical problem that the present invention is intended to solve is to provide a kind of ginsenoside LBP-X Yoghourt chewable tablets and its preparation side
Method.
The present invention solve technical problem scheme be：
A kind of preparation method of ginsenoside LBP-X Yoghourt chewable tablets is as follows：
The extraction of 1.1 ginsenosides
(1) Feedstock treating
Ginseng cleaning, drying, stripping and slicing, crush in Universalpulverizer, and being respectively obtained by the difference of grinding time can be complete
Portion is obtained 100 mesh ginseng pulverates by 100 eye mesh screens；
(2) ultrasound assisted extraction
By ginseng pulverate in above-mentioned (1) under 38.5kHz, ultrasonic extraction time 20-40min, feed liquid mass ratio 1：40-60 adds
Enter water, 40-60 DEG C of temperature, ultrasonic power for 200-300W, filtered while hot after extracting completely；
(3) refine
The macroreticular resin handled well is taken, is loaded in chromatographic column, aqueous extract slowly instills chromatographic column in taking above-mentioned (2), leakage
Go out the speed of liquid for 4mL/min, with the 2-3 times of water wash resin of column volume to efflux in colourless, to wash away polysaccharide therein
Deng water-solubility impurity, then with 70% ethanol solution slowly by resin column, its flow velocity is controlled, so that saponin(e can fully be eluted
Get off, collect eluent, use rotary evaporator concentrate eluant, reclaim ethanol, obtain yellow solid powder；
The extraction of 1.2 LBP-Xs
(1) Feedstock treating
Matrimony vine cleaning, drying, pour into high speed Universal pulverizer and crush 3min, and re-dry is sieved through 100 mesh aperture screen clothes,
Sealing preserve is stand-by；
(2) ultrasound assisted extraction
Matrimony vine in (1) is carried out into ultrasonic extraction, extraction time 20-40min, feed liquid mass ratio 1：40-60 adds water, temperature
40-60 DEG C, ultrasonic power be 200-300W；
(3) centrifugation stands
By ultrasound assisted extraction product centrifugation, retain supernatant, the ethanol for being added thereto to volume fraction 85% is molten
Liquid, can again have precipitation to occur, and precipitation is LBP-X, stands overnight, and filters；
(4) it is vacuum dried
Drying is precipitated to gained under vacuum conditions, LBP-X powder is obtained；
The preparation of 1.3 Yoghourt chewable tablets
(1) preheating, homogeneous, sterilization and cooling
0.5-1.5% ginsenoside powder will be dosed by the percentage by weight of raw material milk in raw material milk, the matrimony vine of 5-15% is more
Icing Sugar, 60 DEG C are preheated to by mixed material, are then then return in sterilisating section in homogeneous is carried out under 20MPa pressure through homogenizer,
80-95 DEG C is warming up to, and 10-25min is kept in soaking zone, then 41-43 DEG C is cooled in cooling section；
(2) it is inoculated with
By said mixture material inoculating starter, leavening is the 0.007% of raw milk quality；
(3) ferment
Postvaccinal material is placed in temperature for 42 DEG C of biochemical cultivation cases carry out fermentation 4-6h；
(4) preparation of sour milk powder
The Yoghourt that will be fermented and its percentage by weight are 10% xylitol, 1.0% magnesium stearate, 1.0% sericin peptide taken are mixed
Close uniform, by after -20 DEG C of pre-freeze 12h, 16-24h being dried under conditions of -50 DEG C of temperature, vacuum 8-10Pa, after lyophilized
Sour milk powder take out, add starch 20%-25%, mix, be divided in the plastic packaging of cleaning bag, because pol is higher slightly viscous
Connect, therefore compartment is ground, it is ensured that fine powder state, crossing 20 mesh sieves carries out whole grain, particle is become more uniform, is easy to the pressure of next step
Piece；
(5) compressing tablet granulating
Compressing tablet machine inlet and drift are carried out disinfection with alcohol before compressing tablet, if having adhesion needs cleaning, Ran Hou in time
By sour milk powder compressing tablet granulating under the pressure of 20kN.
The present invention has the advantage that and good effect：
The present invention mainly have studied the extract with ginseng and matrimony vine as raw material, make ginsenoside LBP-X Yoghourt nozzle
The technique for chewing piece, makes two kinds of material nutrient components bring out the best in each other, and optimize ginseng soap using single factor experiment and orthogonal design
The optimal factory formula of the extraction conditions and Yoghourt chewable tablets of glycosides and LBP-X.Yoghourt is made sour milk tablet, not only can be with
Extend the shelf life, and also have abundant nutritive value and function, be easy to carry instant edible.In the market is most anti-
Oxidation health food is all oral soft capsule class, lacks the food with anti-oxidation function that suitable general population eats, should
Product have be of high nutritive value, processing method is simple and easy to apply, instant the characteristics of.In view of this by ginseng wolfberry fruit extract system
The Yoghourt chewable tablets of work has very big gaps in market and huge development prospect.
Specific embodiment：
With reference to specific embodiment, product of the present invention is expanded on further.It should be understood that embodiment is merely to illustrate the present invention
Rather than limitation the scope of the present invention.
A kind of preparation of ginsenoside LBP-X Yoghourt chewable tablets of example 1
By the ginseng stripping and slicing of cleaning, drying, crushed in Universalpulverizer, being respectively obtained by the difference of grinding time can
All by 0.154mm (100 mesh) screen cloth, 100 mesh ginseng pulverates are obtained.Set ultrasonic power as 200W, 40 DEG C of temperature, solid-liquid ratio
M (ginseng pulverate)：M (distilled water)=1：50th, extract after 40min is extracted completely and filter while hot.Recycle 250mL D101 macropore trees
Fat, is loaded into chromatographic column (3.0cm × 35cm) removal water-solubility impurity, with 70% ethanol elution saponin(e.Use rotary evaporation
Device concentrate eluant, reclaims ethanol, obtains yellow solid powder.
LBP-X is extracted from new fresh fructus lycii through following steps：The cleaning of 20g matrimony vines, drying are taken, high speed ten thousand is poured into
Energy pulverizer crushes 3min, and re-dry is sieved through 0.154mm (100 mesh) aperture screen cloth, sets ultrasonic power as 200W, temperature
50 DEG C, solid-liquid ratio m (wolfberry fruit powder)：M (distilled water)=1：50th, after extracting 30min extractions completely, centrifugation stands, and retains supernatant.
The graded alcohols solution of volume fraction 85% is added thereto to, there is precipitation to occur, precipitation is LBP-X, stands overnight, mistake
Filter.(50 DEG C) dryings, obtain LBP-X powder under vacuum conditions.
Raw milk is standardized by after the purifying steps such as filter cleaner, then carries out molten sugar and dispensing, food ingredient：
Ginsenoside powder addition 0.5g, wolfberry fruit powder addition 10g, raw milk 80mL.Raw milk enters plate heat exchanger through pump
Preheating section, is preheated to 60 DEG C, is then then return in sterilisating section in homogeneous is carried out under 20MPa pressure through homogenizer, is warming up to 90
DEG C, and 25min is kept in soaking zone, then it is cooled to 41 DEG C in cooling section.The leavening (ABY-3) of raw material milk 0.007% is inoculated with,
Above-mentioned material is placed in temperature for 42 DEG C of biochemical cultivation cases carry out fermentation 4-6h again.The Yoghourt that to ferment and 10g xylitols,
1.0g magnesium stearates, 1.0g sericin peptide takens are well mixed, by after -20 DEG C of pre-freeze 12h, in -45 DEG C of temperature, the bar of vacuum 10Pa
16-24h is dried under part.Sour milk powder after will be lyophilized takes out, plus 20g starch, mixes, and is divided in the plastic packaging of cleaning bag, compartment
Grinding, it is ensured that fine powder state, crossing 20 mesh sieves carries out whole grain, then by sour milk powder compressing tablet granulating under the pressure of 20kN.
A kind of preparation of ginsenoside LBP-X Yoghourt chewable tablets of example 2
By the ginseng stripping and slicing of cleaning, drying, crushed in Universalpulverizer, being respectively obtained by the difference of grinding time can
All by 0.154mm (100 mesh) screen cloth, 100 mesh ginseng pulverates are obtained.Set ultrasonic power as 250W, temperature 45 C, solid-liquid ratio
M (ginseng pulverate)：M (distilled water)=1：45th, extract after 40min is extracted completely and filter while hot.Recycle 250mL D101 macropore trees
Fat, is loaded into chromatographic column (3.0cm × 35cm) removal water-solubility impurity, with 70% ethanol elution saponin(e.Use rotary evaporation
Device concentrate eluant, reclaims ethanol, obtains yellow solid powder.
LBP-X is extracted from new fresh fructus lycii through following steps：The cleaning of 20g matrimony vines, drying are taken, high speed ten thousand is poured into
Energy pulverizer crushes 3min, and re-dry is sieved through 0.154mm (100 mesh) aperture screen cloth, sets ultrasonic power as 200W, temperature
50 DEG C, solid-liquid ratio m (wolfberry fruit powder)：M (distilled water)=1：45th, after extracting 30min extractions completely, centrifugation stands, and retains supernatant.
The graded alcohols solution of volume fraction 85% is added thereto to, there is precipitation to occur, precipitation is LBP-X, stands overnight, mistake
Filter.(50 DEG C) dryings, obtain LBP-X powder under vacuum conditions.
Raw milk is standardized by after the purifying steps such as filter cleaner, then carries out molten sugar and dispensing, food ingredient：
Ginsenoside powder addition 1.0g, wolfberry fruit powder addition 12g, raw milk 80mL.Raw milk enters plate heat exchanger through pump
Preheating section, is preheated to 60 DEG C, is then then return in sterilisating section in homogeneous is carried out under 20MPa pressure through homogenizer, is warming up to 90
DEG C, and 25min is kept in soaking zone, then it is cooled to 41 DEG C in cooling section.The leavening (ABY-3) of raw material milk 0.007% is inoculated with,
Above-mentioned material is placed in temperature for 42 DEG C of biochemical cultivation cases carry out fermentation 5h again.The Yoghourt that will be fermented and 10g xylitols, 1.0g
Magnesium stearate, 1.0g sericin peptide takens are well mixed, by after -20 DEG C of pre-freeze 12h, under conditions of -45 DEG C of temperature, vacuum 10Pa
Dry 20h.Sour milk powder after will be lyophilized takes out, plus 20g starch, mixes, and is divided in the plastic packaging of cleaning bag, compartment grinding, really
Fine powder state is protected, crossing 20 mesh sieves carries out whole grain, then by sour milk powder compressing tablet granulating under the pressure of 20kN.
A kind of preparation of ginsenoside LBP-X Yoghourt chewable tablets of example 3
By the ginseng stripping and slicing of cleaning, drying, crushed in Universalpulverizer, being respectively obtained by the difference of grinding time can
All by 0.154mm (100 mesh) screen cloth, 100 mesh ginseng pulverates are obtained.Set ultrasonic power as 200W, 40 DEG C of temperature, solid-liquid ratio
M (ginseng pulverate)：M (distilled water)=1：50th, extract after 30min is extracted completely and filter while hot.Recycle 250mL D101 macropore trees
Fat, is loaded into chromatographic column (3.0cm × 35cm) removal water-solubility impurity, with 70% ethanol elution saponin(e.Use rotary evaporation
Device concentrate eluant, reclaims ethanol, obtains yellow solid powder.
LBP-X is extracted from new fresh fructus lycii through following steps：The cleaning of 20g matrimony vines, drying are taken, high speed ten thousand is poured into
Energy pulverizer crushes 3min, and re-dry is sieved through 0.154mm (100 mesh) aperture screen cloth, sets ultrasonic power as 250W, temperature
45 DEG C, solid-liquid ratio m (wolfberry fruit powder)：M (distilled water)=1：45th, after extracting 30min extractions completely, centrifugation stands, and retains supernatant.
The graded alcohols solution of volume fraction 85% is added thereto to, there is precipitation to occur, precipitation is LBP-X, stands overnight, mistake
Filter.(50 DEG C) dryings, obtain LBP-X powder under vacuum conditions.
Raw milk is standardized by after the purifying steps such as filter cleaner, then carries out molten sugar and dispensing, food ingredient：
Ginsenoside powder addition 0.8g, wolfberry fruit powder addition 15g, raw milk 80mL.Raw milk enters plate heat exchanger through pump
Preheating section, is preheated to 60 DEG C, is then then return in sterilisating section in homogeneous is carried out under 20MPa pressure through homogenizer, is warming up to 90
DEG C, and 25min is kept in soaking zone, then it is cooled to 41 DEG C in cooling section.The leavening (ABY-3) of raw material milk 0.007% is inoculated with,
Above-mentioned material is placed in temperature for 42 DEG C of biochemical cultivation cases carry out fermentation 6h again.The Yoghourt that will be fermented and 10g xylitols, 1.0g
Magnesium stearate, 1.0g sericin peptide takens are well mixed, by after -20 DEG C of pre-freeze 12h, under conditions of -45 DEG C of temperature, vacuum 10Pa
Dry 18h.Sour milk powder after will be lyophilized takes out, plus 20g starch, mixes, and is divided in the plastic packaging of cleaning bag, compartment grinding, really
Fine powder state is protected, crossing 20 mesh sieves carries out whole grain, then by sour milk powder compressing tablet granulating under the pressure of 20kN.
Claims (1)
1. a kind of preparation method of ginsenoside LBP-X Yoghourt chewable tablets, it is characterised in that comprise the following steps：
The extraction of 1.1 ginsenosides
(1) Feedstock treating
Ginseng cleaning, drying, stripping and slicing, are crushed in Universalpulverizer, and being respectively obtained by the difference of grinding time all to be led to
100 eye mesh screens are crossed, 100 mesh ginseng pulverates are obtained；
(2) ultrasound assisted extraction
By ginseng pulverate in above-mentioned (1) under 38.5kHz, ultrasonic extraction time 20-40min, feed liquid mass ratio 1：40-60 is added
Water, 40-60 DEG C of temperature, ultrasonic power are 200-300W, are filtered while hot after extracting completely；
(3) refine
The macroreticular resin handled well is taken, is loaded in chromatographic column, aqueous extract slowly instills chromatographic column, omission timber in taking above-mentioned (2)
Speed be 4mL/min, with the 2-3 times of water wash resin of column volume to efflux in colourless, to wash away the water such as polysaccharide therein
Solubility impurity, then with 70% ethanol solution slowly by resin column, its flow velocity is controlled, so that saponin(e can fully be eluted down
Come, collect eluent, use rotary evaporator concentrate eluant, reclaim ethanol, obtain yellow solid powder；
The extraction of 1.2 LBP-Xs
(1) Feedstock treating
Matrimony vine cleaning, drying, pour into high speed Universal pulverizer and crush 3min, and re-dry is sieved through 100 mesh aperture screen clothes, sealing
Preserve stand-by；
(2) ultrasound assisted extraction
Matrimony vine in (1) is carried out into ultrasonic extraction, extraction time 20-40min, feed liquid mass ratio 1：40-60 adds water, temperature 40-60
DEG C, ultrasonic power be 200-300W；
(3) centrifugation stands
By ultrasound assisted extraction product centrifugation, retain supernatant, be added thereto to the ethanol solution of volume fraction 85%, meeting
There is precipitation to occur again, precipitation is LBP-X, stands overnight, filter；
(4) it is vacuum dried
Drying is precipitated to gained under vacuum conditions, LBP-X powder is obtained；
The preparation of 1.3 Yoghourt chewable tablets
(1) preheating, homogeneous, sterilization and cooling
In raw material milk 0.5-1.5% ginsenoside powder will be dosed by the percentage by weight of raw material milk, the LBP-X powder of 5-15%,
Mixed material is preheated to 60 DEG C, is then then return in sterilisating section in homogeneous is carried out under 20MPa pressure through homogenizer, heated up
To 80-95 DEG C, and 10-25min is kept in soaking zone, then 41-43 DEG C is cooled in cooling section；
(2) it is inoculated with
By said mixture material inoculating starter, leavening is the 0.007% of raw milk quality；
(3) ferment
Postvaccinal material is placed in temperature for 42 DEG C of biochemical cultivation cases carry out fermentation 4-6h；
(4) preparation of sour milk powder
The Yoghourt that will be fermented is 10% xylitol with its percentage by weight, 1.0% magnesium stearate, 1.0% sericin peptide taken mix
It is even, by after -20 DEG C of pre-freeze 12h, 16-24h being dried under conditions of -50 DEG C of temperature, vacuum 8-10Pa, the acid after freezing
Milk powder takes out, and adds starch 20%-25%, mixes, and is divided in the plastic packaging of cleaning bag, because pol slightly adhesion higher, therefore
Compartment is ground, it is ensured that fine powder state, and crossing 20 mesh sieves carries out whole grain, particle is become more uniform, is easy to the compressing tablet of next step；
(5) compressing tablet granulating
Compressing tablet machine inlet and drift are carried out disinfection with alcohol before compressing tablet, if there is the cleaning in time of adhesion needs, then in 20kN
Pressure under by sour milk powder compressing tablet granulating.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108079033A (en) * | 2018-02-06 | 2018-05-29 | 苏州源康食品科技有限公司 | A kind of application of extraction of panax ginseng and its extract in food |
| CN109232762A (en) * | 2018-10-10 | 2019-01-18 | 汕头大学 | A kind of Porphyra haitanensis polysaccharide and preparation method thereof is applied with prebiotic activity |
| CN109998009A (en) * | 2018-10-11 | 2019-07-12 | 浙江杏林堂生物科技有限公司 | A kind of solid beverage of Chinese chestnut peptide and preparation method thereof |
| CN113116946A (en) * | 2019-12-30 | 2021-07-16 | 天津天士力现代中药资源有限公司 | Method for simultaneously preparing red ginseng fibrous root polysaccharide and saponin extraction |
| CN115886104A (en) * | 2022-11-30 | 2023-04-04 | 福建佳德食品有限公司 | Freeze-dried ginseng tea and preparation method thereof |
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| CN105248651A (en) * | 2015-11-23 | 2016-01-20 | 徐州工程学院 | Sweet corn, gingko, blueberry, kiwi fruit and yoghourt chewable tablets and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108079033A (en) * | 2018-02-06 | 2018-05-29 | 苏州源康食品科技有限公司 | A kind of application of extraction of panax ginseng and its extract in food |
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| CN109998009A (en) * | 2018-10-11 | 2019-07-12 | 浙江杏林堂生物科技有限公司 | A kind of solid beverage of Chinese chestnut peptide and preparation method thereof |
| CN113116946A (en) * | 2019-12-30 | 2021-07-16 | 天津天士力现代中药资源有限公司 | Method for simultaneously preparing red ginseng fibrous root polysaccharide and saponin extraction |
| CN115886104A (en) * | 2022-11-30 | 2023-04-04 | 福建佳德食品有限公司 | Freeze-dried ginseng tea and preparation method thereof |
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