CN108567046A - A kind of oyster Semen Maydis polypeptide electuary of anti-fatigue effect and preparation method thereof - Google Patents
A kind of oyster Semen Maydis polypeptide electuary of anti-fatigue effect and preparation method thereof Download PDFInfo
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- CN108567046A CN108567046A CN201810279397.0A CN201810279397A CN108567046A CN 108567046 A CN108567046 A CN 108567046A CN 201810279397 A CN201810279397 A CN 201810279397A CN 108567046 A CN108567046 A CN 108567046A
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- oyster
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- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 65
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 54
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 51
- 241000237502 Ostreidae Species 0.000 title claims abstract description 41
- 235000020636 oyster Nutrition 0.000 title claims abstract description 41
- 230000002929 anti-fatigue Effects 0.000 title claims abstract description 33
- 230000000694 effects Effects 0.000 title claims abstract description 31
- 210000000582 semen Anatomy 0.000 title claims abstract description 20
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 239000000843 powder Substances 0.000 claims abstract description 103
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 33
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 33
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims abstract description 30
- 238000002156 mixing Methods 0.000 claims abstract description 27
- 238000000605 extraction Methods 0.000 claims abstract description 24
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 23
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 23
- 235000005822 corn Nutrition 0.000 claims abstract description 23
- 239000004365 Protease Substances 0.000 claims abstract description 19
- 239000000463 material Substances 0.000 claims abstract description 19
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 claims abstract description 16
- 229930003427 Vitamin E Natural products 0.000 claims abstract description 15
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims abstract description 15
- 235000019165 vitamin E Nutrition 0.000 claims abstract description 15
- 229940046009 vitamin E Drugs 0.000 claims abstract description 15
- 239000011709 vitamin E Substances 0.000 claims abstract description 15
- 108091005804 Peptidases Proteins 0.000 claims abstract description 14
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 14
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims abstract description 14
- 235000011478 zinc gluconate Nutrition 0.000 claims abstract description 14
- WHMDKBIGKVEYHS-IYEMJOQQSA-L Zinc gluconate Chemical compound [Zn+2].OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O.OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O WHMDKBIGKVEYHS-IYEMJOQQSA-L 0.000 claims abstract description 12
- 239000011670 zinc gluconate Substances 0.000 claims abstract description 12
- 229960000306 zinc gluconate Drugs 0.000 claims abstract description 12
- 235000020183 skimmed milk Nutrition 0.000 claims abstract description 11
- 229960003080 taurine Drugs 0.000 claims abstract description 8
- 239000005030 aluminium foil Substances 0.000 claims abstract description 7
- 238000004806 packaging method and process Methods 0.000 claims abstract description 7
- 239000007788 liquid Substances 0.000 claims description 45
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 36
- 241000233866 Fungi Species 0.000 claims description 27
- 238000001035 drying Methods 0.000 claims description 25
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- 239000000203 mixture Substances 0.000 claims description 16
- 102000004190 Enzymes Human genes 0.000 claims description 14
- 108090000790 Enzymes Proteins 0.000 claims description 14
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 14
- 235000013601 eggs Nutrition 0.000 claims description 14
- 229940088598 enzyme Drugs 0.000 claims description 13
- 239000004615 ingredient Substances 0.000 claims description 13
- 235000019419 proteases Nutrition 0.000 claims description 13
- 239000000706 filtrate Substances 0.000 claims description 12
- 238000000227 grinding Methods 0.000 claims description 12
- 238000001556 precipitation Methods 0.000 claims description 11
- 238000005119 centrifugation Methods 0.000 claims description 10
- 239000000287 crude extract Substances 0.000 claims description 10
- 238000001704 evaporation Methods 0.000 claims description 8
- 230000008020 evaporation Effects 0.000 claims description 8
- 239000000758 substrate Substances 0.000 claims description 8
- 238000000108 ultra-filtration Methods 0.000 claims description 8
- 235000015165 citric acid Nutrition 0.000 claims description 7
- 238000001694 spray drying Methods 0.000 claims description 7
- 239000003513 alkali Substances 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 6
- 238000004108 freeze drying Methods 0.000 claims description 6
- 238000000265 homogenisation Methods 0.000 claims description 6
- 238000000746 purification Methods 0.000 claims description 6
- 239000006228 supernatant Substances 0.000 claims description 6
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 6
- 108090000526 Papain Proteins 0.000 claims description 5
- 229940055729 papain Drugs 0.000 claims description 5
- 235000019834 papain Nutrition 0.000 claims description 5
- 238000002203 pretreatment Methods 0.000 claims description 4
- 239000002253 acid Substances 0.000 claims description 3
- 238000009835 boiling Methods 0.000 claims description 2
- 229960004106 citric acid Drugs 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims description 2
- 238000003828 vacuum filtration Methods 0.000 claims description 2
- 229940088594 vitamin Drugs 0.000 claims description 2
- 229930003231 vitamin Natural products 0.000 claims description 2
- 235000013343 vitamin Nutrition 0.000 claims description 2
- 239000011782 vitamin Substances 0.000 claims description 2
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 2
- 241000209149 Zea Species 0.000 claims 2
- 235000005979 Citrus limon Nutrition 0.000 claims 1
- 244000131522 Citrus pyriformis Species 0.000 claims 1
- 240000008042 Zea mays Species 0.000 abstract description 21
- 238000000034 method Methods 0.000 abstract description 14
- 239000002994 raw material Substances 0.000 abstract description 8
- 150000001875 compounds Chemical class 0.000 abstract description 7
- 238000012545 processing Methods 0.000 abstract description 5
- 230000002035 prolonged effect Effects 0.000 abstract description 5
- 239000000047 product Substances 0.000 abstract description 3
- 239000006227 byproduct Substances 0.000 abstract description 2
- 235000018102 proteins Nutrition 0.000 description 26
- 238000005516 engineering process Methods 0.000 description 19
- 150000004676 glycans Chemical class 0.000 description 17
- 229920001282 polysaccharide Polymers 0.000 description 17
- 239000005017 polysaccharide Substances 0.000 description 17
- 241000221377 Auricularia Species 0.000 description 9
- 235000013305 food Nutrition 0.000 description 6
- 238000011160 research Methods 0.000 description 6
- 235000019441 ethanol Nutrition 0.000 description 5
- 230000036039 immunity Effects 0.000 description 5
- MVORZMQFXBLMHM-QWRGUYRKSA-N Gly-His-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)CN)CC1=CN=CN1 MVORZMQFXBLMHM-QWRGUYRKSA-N 0.000 description 4
- 239000002671 adjuvant Substances 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 230000006870 function Effects 0.000 description 4
- 108010038983 glycyl-histidyl-lysine Proteins 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 235000000023 Auricularia auricula Nutrition 0.000 description 3
- 241001149430 Auricularia auricula-judae Species 0.000 description 3
- 102100025292 Stress-induced-phosphoprotein 1 Human genes 0.000 description 3
- 235000001014 amino acid Nutrition 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 230000000259 anti-tumor effect Effects 0.000 description 3
- 239000003963 antioxidant agent Substances 0.000 description 3
- 230000003078 antioxidant effect Effects 0.000 description 3
- 235000006708 antioxidants Nutrition 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 2
- 239000005864 Sulphur Substances 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 235000013376 functional food Nutrition 0.000 description 2
- 235000013402 health food Nutrition 0.000 description 2
- 238000002386 leaching Methods 0.000 description 2
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- 230000035479 physiological effects, processes and functions Effects 0.000 description 2
- 150000003254 radicals Chemical class 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000012795 verification Methods 0.000 description 2
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 1
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 206010001497 Agitation Diseases 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 description 1
- 244000241257 Cucumis melo Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- AHLPHDHHMVZTML-BYPYZUCNSA-N L-Ornithine Chemical compound NCCC[C@H](N)C(O)=O AHLPHDHHMVZTML-BYPYZUCNSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 108010038807 Oligopeptides Proteins 0.000 description 1
- 102000015636 Oligopeptides Human genes 0.000 description 1
- AHLPHDHHMVZTML-UHFFFAOYSA-N Orn-delta-NH2 Natural products NCCCC(N)C(O)=O AHLPHDHHMVZTML-UHFFFAOYSA-N 0.000 description 1
- UTJLXEIPEHZYQJ-UHFFFAOYSA-N Ornithine Natural products OC(=O)C(C)CCCN UTJLXEIPEHZYQJ-UHFFFAOYSA-N 0.000 description 1
- 241001506047 Tremella Species 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000003178 anti-diabetic effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000010100 anticoagulation Effects 0.000 description 1
- 239000003472 antidiabetic agent Substances 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 238000010411 cooking Methods 0.000 description 1
- 235000020247 cow milk Nutrition 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 235000014103 egg white Nutrition 0.000 description 1
- 210000000969 egg white Anatomy 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000005714 functional activity Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 235000020256 human milk Nutrition 0.000 description 1
- 210000004251 human milk Anatomy 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000000874 microwave-assisted extraction Methods 0.000 description 1
- 239000003595 mist Substances 0.000 description 1
- 239000011812 mixed powder Substances 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 229960003104 ornithine Drugs 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000009711 regulatory function Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/15—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/16—Inorganic salts, minerals or trace elements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/175—Amino acids
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Mycology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Inorganic Chemistry (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Oyster Semen Maydis polypeptide electuary of anti-fatigue effect of the present invention and preparation method thereof belongs to agricultural and sideline product intensive processing and its byproduct comprehensive utilization technical field, is related to a kind of oyster Semen Maydis polypeptide electuary of anti-fatigue effect and preparation method thereof.Using protein content be all higher than 80% Oyster Protein powder and corn protein powder as raw material, molecular weight is obtained in 5000 Da mixed polypeptide powder below by compound protease Controlled-enzymatic Hydrolysis method, mixed polypeptide powder is verified by zoopery in 120 days, it can be obviously prolonged the run duration of mouse, alleviate organism fatigue；Using Blackfungus polyhexose made from autoclaving extraction as major auxiliary burden; the main function of Blackfungus polyhexose is the solubility for increasing polypeptide; and there is anti-fatigue effect; it is additionally added the auxiliary materials such as skimmed milk powder, taurine, zinc gluconate, vitamin E; mixing allotment is carried out, is packed using the packaging bag of aluminium foil material, every bag of 25 ± 2 g; a kind of oyster Semen Maydis polypeptide electuary of anti-fatigue effect was made in 18 months shelf-lifves.
Description
Technical field
The invention belongs to agricultural and sideline product intensive processing and its technical fields of byproduct comprehensive utilization, are related to a kind of antifatigue
Oyster Semen Maydis polypeptide electuary of function and preparation method thereof.
Background technology
With the development of science and technology, oyster meat is handled with various technological means, as zymolysis technique, food industry are fermented
Deng by these technological means, oyster extraction product can be obtained, wherein containing a large amount of oligopeptides, polypeptide, amino acid etc. has
The nutritional ingredient of functional activity.It is many studies have shown that the weight ratio and completeness of the essential amino acid in oyster, male
Nutrition in oyster may be better than human milk and cow's milk.In oyster peptide, except 20 kinds of necessary amino acid of needed by human body, also contain one
The very high amino acid of a little nutritive values, such as ornithine, taurine.Wherein, content of taurine is apparently higher than other animalities food
Product belong to a kind of active material, have important Physiology and biochemistry activity, such as antifatigue, anti-oxidant, antitumor, hypoglycemic raw
Object activity, is widely used in food service industry.
Corn is the traditional grain crop in China, is had a very wide distribution, the rapid development of the industry of corn intensive processing in recent years, battalion
It is abundant to support value, equally, there are many processing technologies to be applied to the intensive processing of corn, corn peptide has many biologies living
Property, such as anti-oxidant, antitumor, the bioactivity such as antifatigue.It is many study found that corn functional peptides can reduce the fortune of mouse
Ejector half fatigue contributes to the recovery of mouse body movement ability and increased exercise times, has delay fatigue to generate and promote mouse
The effect of feeling of fatigue is eliminated.Also research confirms, corn functional peptides can improve excitability amino（Glutamic acid）Internal conversion,
It proves that corn functional peptides can adjust mouse organism metabolism, and repairs the physical power loss during movement, therefore, in antifatigue food
Industry has good application prospect.
Blackfungus polyhexose is very extensive in food service industry application, and the bioactivity research about Blackfungus polyhexose has very much, black
The bioactivity of agaric is essentially from its polysaccharide component.Blackfungus polyhexose has the cytoprotections such as anticoagulation, antitumor, anti-inflammatory
Effect, also having reduces a variety of physiology such as blood fat, cholesterol, blood glucose, blood viscosity and anti-diabetic, radioresistance, anti-aging
Function, correlative study prove that polysaccharide has anti-fatigue effect by the Loaned swimming test and rotating stick test of mouse.
In polypeptide and Polyose extraction technical aspect, there are still many drawbacks, such as low yield, easy caking, poor activity.This is specially
The method that profit enzymolysis oyster peptide and corn peptide use compound protease Controlled-enzymatic Hydrolysis, is carried out by vacuum and low temperature spray drying technology
Dry, in drying process, low temperature ensures that the activity of peptide is influenced by minimum, and the biggest advantage is to prevent Gly-His-Lys knot for the technology
Block.For the saccharoidal extraction of bacterium, there are many methods, the technique of alkalinity extraction is simple compared to other, effectively reduces into
This input, but to the more demanding of pH in entire experiment or production, it is such as improper to condition control easily polysaccharide to be made to inactivate.Microwave
Extraction there are certain requirements instrument and equipment, and cost also improves therewith.Have research using autoclaving extraction to white fungus at
After reason, tremella polysaccharides yield is not only increased compared to atmospheric cooking, and shorten process cycle, is carried for the polysaccharide of other mushrooms
It takes and provides more effective ways.The extraction of this patent Auricularia polysaccharide uses autoclaving extraction, the best extraction of optimization
Technique provides theoretical foundation for the extraction of Auricularia polysaccharide.
In conclusion oyster peptide, corn peptide and Auricularia polysaccharide have many physiological functions, embodies a concentrated reflection of adjusting and exempt from
Epidemic disease, antifatigue and anti-oxidant aspect.In the fast-developing social environment that people lives in for a long time, body will appear discomfort, be immunized
Regulatory function may be disorderly, is easy fatigue, therefore it is that have very much must to research and develop a food for adjusting the functions such as immune, antifatigue
It wants.
The technical advantage of the present invention is mainly reflected in following three aspects：
First, it applies for a patent and a kind of oyster Semen Maydis polypeptide electuary of anti-fatigue effect and preparation method thereof is provided, it can extensive use
In health food, army's functional food exploitation etc..
Second, the raw material innovation applied for a patent.80% Oyster Protein powder and corn protein powder are all higher than with protein content
For raw material, molecular weight is obtained in 5000 Da or less mixed polypeptide powder, mixed polypeptide powder by compound protease Controlled-enzymatic Hydrolysis method
It is verified by zoopery in 120 days, the run duration of mouse can be obviously prolonged, alleviate organism fatigue；With autoclaving
Auricularia polysaccharide made from extraction is major auxiliary burden, and water-soluble Auricularia polysaccharide can also increase the solubility of peptide, to make electuary
State it is more preferable, be also added into vitamin E and zinc gluconate, wherein vitamin E is used to put forward the inoxidizability of electuary, removes people
Internal free radical, Zn-ef ficiency can enhance human immunity, and improve organism quality.
Third, the patented technology method diversification of application.It is with ultramicro grinding, vacuum and low temperature spray drying, compound protein
Enzyme Controlled-enzymatic Hydrolysis, autoclaving extraction etc. are core technology.
Its technical advantage protrusion is embodied in：
(1) ultramicro grinding and vacuum and low temperature spray drying technology can effectively reduce the moisture in Gly-His-Lys, and can be with
The active ingredient in Gly-His-Lys is protected very well.
(2) compound protease Controlled-enzymatic Hydrolysis technology combines three kinds of protease, due to hydrolytic sites difference, to improve
The degree of hydrolysis of albumen powder, obtains the smaller Gly-His-Lys of molecular weight, it is easier to be absorbed by the body.
(3) it uses autoclaving microwave leaching technology to handle agaric, improves the yield of polysaccharide, and shorten process cycle,
Water-soluble Auricularia polysaccharide is added in electuary, can increase the solubility of peptide, prevents electuary from generating precipitation in the process that reconstitutes.
(4) zinc gluconate and vitamin E are added in auxiliary material, Zn-ef ficiency can enhance human immunity, and improve body
Quality, beneficial to health, vitamin E is used to improve the inoxidizability of electuary, removes the free radical in human body.
It is reported with technology in conclusion making a general survey of domestic and international correlative study, 80% Oyster Protein is all higher than with protein content
Powder and corn protein powder are raw material, are not yet found by water-soluble polysaccharide to increase the research of peptide solubility.Moreover, also having no
Ultramicro grinding, vacuum and low temperature spray drying, compound protease Controlled-enzymatic Hydrolysis and autoclaving microwave leaching technology is combined
Research.Therefore, this patent provides a kind of preparation method of the oyster Semen Maydis polypeptide electuary of anti-fatigue effect, equal with protein content
Oyster Protein powder and corn protein powder more than 80% are raw material, obtain molecular weight by hop protein enzyme Controlled-enzymatic Hydrolysis method and exist
5000 Da mixed polypeptide powder, mixed polypeptide powder are verified by zoopery in 120 days, can be obviously prolonged the movement of mouse
Time alleviates organism fatigue；Using Auricularia polysaccharide made from autoclaving extraction as major auxiliary burden, it is additionally added skimmed milk powder, ox
The electuary with strengthen immunity, anti-fatigue effect is made in the auxiliary materials such as sulfonic acid, zinc gluconate, vitamin E.
Invention content
Technical problems to be solved needed for application invention：Disclose a kind of anti-fatigue effect oyster Semen Maydis polypeptide electuary and its
Preparation method, which is characterized in that using protein content be all higher than 80% Oyster Protein powder and corn protein powder as raw material, pass through
Hop protein enzyme Controlled-enzymatic Hydrolysis method obtains molecular weight in 5000 Da mixed polypeptide powder, and mixed polypeptide powder passes through 120 days animals
Experimental verification can be obviously prolonged the run duration of mouse, alleviate organism fatigue；With black fungus made from autoclaving extraction
Polysaccharide is major auxiliary burden, is additionally added the auxiliary materials such as skimmed milk powder, taurine, zinc gluconate, vitamin E, is made and exempts from enhancing
The electuary of epidemic disease power, anti-fatigue effect.
Applying for the technical solution of invention is：
A kind of preparation method of the oyster Semen Maydis polypeptide electuary of anti-fatigue effect, which is characterized in that Oyster Protein powder and corn egg
White powder mixes, and obtains egg mix white powder, is spray-dried, obtains by ultramicro grinding, complex enzyme Controlled-enzymatic Hydrolysis, ultrafiltration, vacuum and low temperature
To molecular weight in 5000 Da mixed polypeptide powder below；After black fungus pre-treatment, feed liquid mixing, then autoclaving extraction, mistake
Filter is concentrated by evaporation, collects precipitation, you can Blackfungus polyhexose crude extract is obtained, using purifying drying, you can it is more to obtain black fungus
Sugar；Mixed polypeptide powder, Blackfungus polyhexose and skimmed milk powder, taurine, zinc gluconate, vitamin E, citric acid are mixed,
It is packed using the packaging bag of aluminium foil material, every bag of 25 ± 2 g, 18 months shelf-lifves, a kind of the male of anti-fatigue effect is made
Oyster Semen Maydis polypeptide electuary；
1）The egg mix white powder refers to that protein content is all higher than 80% Oyster Protein powder with corn protein powder according to weight
Measure ratio 1:1 mixing；
2）The ultramicro grinding, egg mix white powder are crushed using supersonic jet mill system, feed stream 0.4-
0.7 MPa, crushing air-flow are 0.5-0.9 MPa, finally obtain mixing ultra micro albumen powder；
3）The complex enzyme Controlled-enzymatic Hydrolysis will mix ultra micro albumen powder, be dissolved in the water, according to weight percent, by substrate
Concentration is adjusted to 4-8 %, and homogenization is heated to 85-95 DEG C, keeps 9-15 min, is subsequently cooled to 45-60 DEG C, uses
The pH value that 1mol/L HCl and NaOH solution adjust solution is 6-10, and it is 1 that weight ratio is added into solution:1:1 trypsase,
Alkali protease and papain, it is 3-10 % to make enzyme-to-substrate weight ratio, persistently enzymolysis 2-5 h, after enzymolysis, is carried out
Enzymolysis liquid is heated to 85-95 DEG C, after keeping 9-15 min, is cooled to room temperature, 4 DEG C of pelleted by centrifugation 10-20 by destroy the enzyme treatment
Min leaves and takes clear liquid, and it is 7.00 ± 0.05 to adjust supernatant pH value with 1mol/L HCl and NaOH solution；
4）Filtrate after enzymolysis is crossed film by the ultrafiltration, passes sequentially through molecular cut off 10000 Da and 5000 Da
Film obtains molecular weight in 5000 Da filtrates below；
5) the vacuum and low temperature spray drying described in, refers to that protein liquid is dried, protein liquid is spray-dried a concentration of 15-
25%, vacuum degree reaches -0.03-0.07 MPa, inlet air temperature is 100-120 DEG C, charging rate is 3-6 mL/min, drying aid
Dosage is 2-5%；
6）The black fungus pre-treatment, black fungus are cleaned up, are then put it into drying box, are drying temperature with 50 DEG C
Degree, crushes after drying, after crossing 80,100 or 120 mesh sieve, obtains black fungus powder；
7）The feed liquid mixing, refers to that black fungus powder is mixed with distilled water according to weight ratio, solid-liquid ratio 60:1-90:
1；
8）The autoclaving extraction, by black fungus powder, carries out boiling, pressure 0.05-1.05 under high pressure
MPa, high-voltage time are 40-60 min；
9）The filtering refers to the black fungus liquid of autoclaving, is handled by vacuum filtration, leave and take filtrate；
10）The evaporation and concentration refers to that filtered filtrate is concentrated, and the volume after concentration is the 40- of initial volume
55%;
11）The collection precipitation, refers to the ethanol solution for being added 95%, 12 h is placed overnight under the conditions of 4 DEG C, 4 DEG C later
Under the conditions of centrifuge 20 min and collect precipitation, i.e. Blackfungus polyhexose crude extract；
12）The purifying drying, refers to Blackfungus polyhexose crude extract, is handled using Sevage solution purifications after adding water to redissolve,
Again through vacuum freeze drying, wherein feed liquid thickness is 3 mm, and in -80 DEG C of refrigerators, the pre-freeze time is 8-15 h, and freeze-drying time is
20-28 h, last arrives Blackfungus polyhexose.
A kind of oyster Semen Maydis polypeptide electuary of anti-fatigue effect, which is characterized in that major ingredient is according to weight proportion, including 85-
92% polypeptide mixed powder, 8-15% Blackfungus polyhexoses.
The oyster Semen Maydis polypeptide electuary of a kind of anti-fatigue effect, which is characterized in that auxiliary material is according to weight proportion, packet
Include 80-85% skimmed milk powders, 8-12% taurines, 1-2% zinc gluconates, 3-6% vitamin Es, 1-4% citric acids.
The oyster Semen Maydis polypeptide electuary of a kind of anti-fatigue effect, which is characterized in that 5-10 is added in every 100 g major ingredients
G auxiliary materials.
The technology of the present invention effect：
(1) it applies for a patent and a kind of oyster Semen Maydis polypeptide electuary of anti-fatigue effect and preparation method thereof is provided, be made with enhancing
The electuary of immunity, anti-fatigue effect can be widely applied to health food, army's functional food exploitation etc..
(2) the raw material innovation applied for a patent, 80% Oyster Protein powder and corn protein powder are all higher than with protein content
For raw material, molecular weight is obtained in 5000 Da mixed polypeptide powder by hop protein enzyme Controlled-enzymatic Hydrolysis method, mixed polypeptide powder has passed through
Zoopery verification in 120 days, can be obviously prolonged the run duration of mouse, alleviate organism fatigue；With autoclaving extraction
Auricularia polysaccharide obtained is major auxiliary burden, and water-soluble Auricularia polysaccharide can also increase the solubility of peptide, to make the state of electuary
More preferably, it is also added into vitamin E and zinc gluconate, vitamin E is used for the inoxidizability of electuary, removes the freedom in human body
Base, Zn-ef ficiency can enhance human immunity, and improve organism quality.
(3) the patented technology method diversification applied is with ultramicro grinding, vacuum and low temperature spray drying, compound protease
Controlled-enzymatic Hydrolysis, autoclaving extraction etc. are core technology.
Specific implementation mode
Embodiment 1：
A certain amount of protein content is all higher than 80% Oyster Protein powder with corn protein powder according to weight ratio 1:1 mixing, obtains
Egg mix white powder, egg mix white powder pass through ultramicro grinding, and feed stream is 0.5 MPa, and crushing air-flow is 0.6 MPa, final
To mixing ultra micro albumen powder；The mixing ultra micro albumen powder of constant weight is weighed again, it is soluble in water, according to weight percent, the bottom of by
Object concentration is adjusted to 5 %, and homogenization is heated to 90 DEG C, keeps 12 min, 50 DEG C is subsequently cooled to, with 1mol/L HCl
The pH value that solution is adjusted with NaOH solution is 8, and it is 1 that weight ratio is added into solution:1:1 trypsase, alkali protease and
Papain, it is 6 % to make enzyme-to-substrate weight ratio, persistently digests 4 h, after enzymolysis, destroy the enzyme treatment is carried out, by enzymolysis liquid
90 DEG C are heated to, after keeping 10 min, is cooled to room temperature, 4 DEG C of 15 min of pelleted by centrifugation leave and take clear liquid, with 1mol/L HCl
It is that 7.00 ± 0.05 enzymolysis liquids carry out ultrafiltration to adjust supernatant pH value with NaOH solution, passes sequentially through 10000 Da and 5000 Da
Film, molecular weight is dried in 5000 Da filtrates below by the technology that vacuum and low temperature is spray-dried, wherein protein liquid
It is spray-dried that a concentration of 20%, vacuum degree reaches -0.02 MPa, inlet air temperature is 110 DEG C, charging rate is 5 mL/min, helps
Dry agent dosage is 3%, finally obtains molecular weight in 5000 Da mixed polypeptide powder below；Good black fungus is selected, cleaning is dry
Only, it then puts it into drying box with 50 DEG C as drying temperature, is crushed after drying, cross 120 mesh sieve, obtain the black of certain particle size
Auricularia auriculajudae powder, according to solid-liquid ratio 70:1 distilled water is added into black fungus powder, carries out autoclaving extraction, and wherein pressure is 1.00
MPa, high-voltage time be 50 min, after be filtered and be concentrated by evaporation be added to the 50% of original volume 95% ethyl alcohol it is molten
Liquid is placed 12 hours overnight under the conditions of 4 DEG C, and precipitation, i.e. Blackfungus polyhexose crude extract are collected after 4 DEG C of 20 min of pelleted by centrifugation,
It is handled using Sevage solution purifications after adding water to redissolve, then through vacuum freeze drying, wherein feed liquid thickness is 3 mm, at -80 DEG C
In refrigerator, the pre-freeze time is 12 h, and freeze-drying time is 24 h, finally obtains Blackfungus polyhexose；All dispensings are according to weight hundred
Divide than mixing, major ingredient proportioning is 90% mixed polypeptide powder, 10% Blackfungus polyhexose；Ratio of adjuvant is 82% skimmed milk powder, 12% N of sulphur
Acid, 1.5% zinc gluconate, 3.5% vitamin E, 1% citric acid；5-10 g auxiliary materials are added in every 100 g major ingredients, by all dispensings
It mixes, is packed using the packaging bag of aluminium foil material according to a certain percentage, every bag of 25 ± 2 g, are made one at 18 months shelf-lifves
The oyster Semen Maydis polypeptide electuary of kind anti-fatigue effect.
Embodiment 2：
A certain amount of protein content is all higher than 80% Oyster Protein powder with corn protein powder according to weight ratio 1:1 mixing, obtains
Egg mix white powder, egg mix white powder pass through ultramicro grinding, and feed stream is 0.4 MPa, and crushing air-flow is 0.5 MPa, final
To mixing ultra micro albumen powder；The mixing ultra micro albumen powder of constant weight is weighed again, it is soluble in water, according to weight percent, the bottom of by
Object concentration is adjusted to 4 %, homogenization, is heated to 95 DEG C, keeps 9 min, is subsequently cooled to 45 DEG C, with 1mol/L HCl and
The pH value that NaOH solution adjusts solution is 6, and it is 1 that weight ratio is added into solution:1:1 trypsase, alkali protease and wood
Melon protease, it is 3% to make enzyme-to-substrate weight ratio, persistently digests 2 h, after enzymolysis, carries out destroy the enzyme treatment, by enzymolysis liquid plus
Heat after keeping 9 min, is cooled to room temperature to 85 DEG C, and 4 DEG C of 10 min of pelleted by centrifugation leave and take clear liquid, with 1mol/L HCl and
It is that 7.00 ± 0.05 enzymolysis liquids carry out ultrafiltration that NaOH solution, which adjusts supernatant pH value, passes sequentially through 10000 Da's and 5000 Da
Molecular weight is dried in 5000 Da filtrates below by the technology that vacuum and low temperature is spray-dried film, and wherein protein liquid sprays
Mist dries a concentration of 15%, vacuum degree reaches -0.03 MPa, inlet air temperature is 100 DEG C, charging rate is 3 mL/min, it is dry to help
Agent dosage is 2%, finally obtains molecular weight in 5000 Da mixed polypeptide powder below；Good black fungus is selected, is cleaned up,
Then it puts it into drying box with 50 DEG C as drying temperature, is crushed after drying, cross 80 mesh sieve, obtain the black fungus of certain particle size
Powder, according to solid-liquid ratio 60:1 is added distilled water into black fungus powder, carries out autoclaving extraction, wherein pressure be 0.05 MPa,
High-voltage time be 40 min, after be filtered and be concentrated by evaporation to 40 % of original volume, 95% ethanol solution is added,
It is placed overnight under the conditions of 4 DEG C 12 hours, precipitation, i.e. Blackfungus polyhexose crude extract is collected after 4 DEG C of 20 min of pelleted by centrifugation, is added
Water is handled after redissolving using Sevage solution purifications, then through vacuum freeze drying, wherein feed liquid thickness is 3 mm, in -80 DEG C of ice
In case, the pre-freeze time is 15 h, and freeze-drying time is 20 h, finally obtains Blackfungus polyhexose；All dispensings are according to weight percent
Than mixing, major ingredient proportioning is 85% mixed polypeptide powder, 15% Blackfungus polyhexose；Ratio of adjuvant be 80% skimmed milk powder, 8% taurine,
2% zinc gluconate, 6% vitamin E, 4% citric acid；5 g auxiliary materials are added in every 100 g major ingredients, by all dispensings according to certain ratio
Example mixing, is packed, every bag of 25 ± 2 g, a kind of antifatigue work(was made in 18 months shelf-lifves using the packaging bag of aluminium foil material
The oyster Semen Maydis polypeptide electuary of energy.
Embodiment 3：
A certain amount of protein content is all higher than 80% Oyster Protein powder with corn protein powder according to weight ratio 1:1 mixing, obtains
Egg mix white powder, egg mix white powder pass through ultramicro grinding, and feed stream is 0.7 MPa, and crushing air-flow is 0.9 MPa, final
To mixing ultra micro albumen powder；The mixing ultra micro albumen powder of constant weight is weighed again, it is soluble in water, according to weight percent, the bottom of by
Object concentration is adjusted to 8 %, and homogenization is heated to 85 DEG C, keeps 15 min, 60 DEG C is subsequently cooled to, with 1mol/L HCl
The pH value that solution is adjusted with NaOH solution is 10, and it is 1 that weight ratio is added into solution:1:1 trypsase, alkali protease and
Papain, it is 10 % to make enzyme-to-substrate weight ratio, persistently digests 5 h, after enzymolysis, carries out destroy the enzyme treatment, will digest
Liquid is heated to 95 DEG C, after keeping 15 min, is cooled to room temperature, 4 DEG C of 20 min of pelleted by centrifugation leave and take clear liquid, use 1mol/L
It is that 7.00 ± 0.05 enzymolysis liquids carry out ultrafiltration that HCl and NaOH solution, which adjust supernatant pH value, passes sequentially through 10000 Da and 5000
The film of Da molecular weight is dried in 5000 Da filtrates below by the technology that vacuum and low temperature is spray-dried, wherein albumen
Liquid is spray-dried a concentration of 25%, vacuum degree reaches 0.07 MPa, inlet air temperature is 120 DEG C, charging rate is 6 mL/min, helps
Dry agent dosage is 5%, finally obtains molecular weight in 5000 Da mixed polypeptide powder below；Good black fungus is selected, cleaning is dry
Only, it then puts it into drying box with 50 DEG C as drying temperature, is crushed after drying, cross 80 mesh sieve, obtain the black of certain particle size
Auricularia auriculajudae powder, according to solid-liquid ratio 90:1 distilled water is added into black fungus powder, carries out autoclaving extraction, and wherein pressure is 1.05
MPa, high-voltage time be 60 min, after be filtered and be concentrated by evaporation be added to the 55% of original volume 95% ethyl alcohol it is molten
Liquid is placed 12 hours overnight under the conditions of 4 DEG C, and precipitation, i.e. Blackfungus polyhexose crude extract are collected after 4 DEG C of 20 min of pelleted by centrifugation,
It is handled using Sevage solution purifications after adding water to redissolve, then through vacuum freeze drying, wherein feed liquid thickness is 3 mm, at -80 DEG C
In refrigerator, the pre-freeze time is 8 h, and freeze-drying time is 28 h, finally obtains Blackfungus polyhexose；All dispensings are according to weight percent
Than mixing, major ingredient proportioning is 92% mixed polypeptide powder, 8% Blackfungus polyhexose；Ratio of adjuvant is 85% skimmed milk powder, 9% taurine, 1%
Zinc gluconate, 3% vitamin E, 2% citric acid；10 g auxiliary materials are added in every 100 g major ingredients, by all dispensings according to certain ratio
Example mixing, is packed, every bag of 25 ± 2 g, a kind of antifatigue work(was made in 18 months shelf-lifves using the packaging bag of aluminium foil material
The oyster Semen Maydis polypeptide electuary of energy.
Embodiment 4：
A certain amount of protein content is all higher than 80% Oyster Protein powder with corn protein powder according to weight ratio 1:1 mixing, obtains
Egg mix white powder, egg mix white powder pass through ultramicro grinding, and feed stream is 0.5 MPa, and crushing air-flow is 0.6 MPa, final
To mixing ultra micro albumen powder；The mixing ultra micro albumen powder of constant weight is weighed again, it is soluble in water, according to weight percent, the bottom of by
Object concentration is adjusted to 5 %, and homogenization is heated to 90 DEG C, keeps 12 min, 50 DEG C is subsequently cooled to, with 1mol/L HCl
The pH value that solution is adjusted with NaOH solution is 8, and it is 1 that weight ratio is added into solution:1:1 trypsase, alkali protease and
Papain, it is 6 % to make enzyme-to-substrate weight ratio, persistently digests 4 h, after enzymolysis, destroy the enzyme treatment is carried out, by enzymolysis liquid
90 DEG C are heated to, after keeping 10 min, is cooled to room temperature, 4 DEG C of 15 min of pelleted by centrifugation leave and take clear liquid, with 1mol/L HCl
It is that 7.00 ± 0.05 enzymolysis liquids carry out ultrafiltration to adjust supernatant pH value with NaOH solution, passes sequentially through 10000 Da and 5000 Da
Film, molecular weight is dried in 5000 Da filtrates below by the technology that vacuum and low temperature is spray-dried, wherein protein liquid
It is spray-dried that a concentration of 20%, vacuum degree reaches -0.02 MPa, inlet air temperature is 110 DEG C, charging rate is 5 mL/min, helps
Dry agent dosage is 3%, finally obtains molecular weight in 5000 Da mixed polypeptide powder below；Good black fungus is selected, cleaning is dry
Only, it then puts it into drying box with 50 DEG C as drying temperature, crushes, sieve with 100 mesh sieve after drying, obtain the black of certain particle size
Auricularia auriculajudae powder, according to solid-liquid ratio 70:1 distilled water is added into black fungus powder, carries out autoclaving extraction, and wherein pressure is 1.00
MPa, high-voltage time be 50 min, after be filtered and be concentrated by evaporation be added to the 50% of original volume 95% ethyl alcohol it is molten
Liquid is placed 12 hours overnight under the conditions of 4 DEG C, and precipitation, i.e. Blackfungus polyhexose crude extract are collected after 4 DEG C of 20 min of pelleted by centrifugation,
It is handled using Sevage solution purifications after adding water to redissolve, then through vacuum freeze drying, wherein feed liquid thickness is 3 mm, at -80 DEG C
In refrigerator, the pre-freeze time is 12 h, and freeze-drying time is 24 h, finally obtains Blackfungus polyhexose；All dispensings are according to weight hundred
Divide than mixing, major ingredient proportioning is 90% mixed polypeptide powder, 10% Blackfungus polyhexose；Ratio of adjuvant is 82% skimmed milk powder, 12% N of sulphur
Acid, 1% zinc gluconate, 4% vitamin E, 1% citric acid；5-10 g auxiliary materials are added in every 100 g major ingredients, by all dispensings according to
Certain proportion mixes, and is packed using the packaging bag of aluminium foil material, every bag of 25 ± 2 g, 18 months shelf-lifves, is made a kind of anti-
The oyster Semen Maydis polypeptide electuary of fatigue function.
Claims (4)
1. a kind of preparation method of the oyster Semen Maydis polypeptide electuary of anti-fatigue effect, which is characterized in that Oyster Protein powder and corn
Albumen powder mixes, and obtains egg mix white powder, is spray-dried by ultramicro grinding, complex enzyme Controlled-enzymatic Hydrolysis, ultrafiltration, vacuum and low temperature,
Molecular weight is obtained in 5000 Da mixed polypeptide powder below；After black fungus pre-treatment, feed liquid mixing, then autoclaving extraction, mistake
Filter is concentrated by evaporation, collects precipitation, you can Blackfungus polyhexose crude extract is obtained, using purifying drying, you can it is more to obtain black fungus
Sugar；Mixed polypeptide powder, Blackfungus polyhexose and skimmed milk powder, taurine, zinc gluconate, vitamin E, citric acid are mixed,
It is packed using the packaging bag of aluminium foil material, every bag of 25 ± 2 g, 18 months shelf-lifves, a kind of the male of anti-fatigue effect is made
Oyster Semen Maydis polypeptide electuary；
1）The egg mix white powder refers to that protein content is all higher than 80% Oyster Protein powder with corn protein powder according to weight
Measure ratio 1:1 mixing；
2）The ultramicro grinding, egg mix white powder are crushed using supersonic jet mill system, feed stream 0.4-
0.7 MPa, crushing air-flow are 0.5-0.9 MPa, finally obtain mixing ultra micro albumen powder；
3）The complex enzyme Controlled-enzymatic Hydrolysis will mix ultra micro albumen powder, be dissolved in the water, according to weight percent, by substrate
Concentration is adjusted to 4-8 %, and homogenization is heated to 85-95 DEG C, keeps 9-15 min, is subsequently cooled to 45-60 DEG C, uses
The pH value that 1mol/L HCl and NaOH solution adjust solution is 6-10, and it is 1 that weight ratio is added into solution:1:1 trypsase,
Alkali protease and papain, it is 3-10 % to make enzyme-to-substrate weight ratio, persistently enzymolysis 2-5 h, after enzymolysis, is carried out
Enzymolysis liquid is heated to 85-95 DEG C, after keeping 9-15 min, is cooled to room temperature, 4 DEG C of pelleted by centrifugation 10-20 by destroy the enzyme treatment
Min leaves and takes clear liquid, and it is 7.00 ± 0.05 to adjust supernatant pH value with 1mol/L HCl and NaOH solution；
4）Filtrate after enzymolysis is crossed film by the ultrafiltration, passes sequentially through molecular cut off 10000 Da and 5000 Da
Film obtains molecular weight in 5000 Da filtrates below；
5) the vacuum and low temperature spray drying described in, refers to that protein liquid is dried, protein liquid is spray-dried a concentration of 15-
25%, vacuum degree reaches -0.03-0.07 MPa, inlet air temperature is 100-120 DEG C, charging rate is 3-6 mL/min, drying aid
Dosage is 2-5%；
6）The black fungus pre-treatment, black fungus are cleaned up, are then put it into drying box, are drying temperature with 50 DEG C
Degree, crushes after drying, after crossing 80,100 or 120 mesh sieve, obtains black fungus powder；
7）The feed liquid mixing, refers to that black fungus powder is mixed with distilled water according to weight ratio, solid-liquid ratio 60:1-90:
1；
8）The autoclaving extraction, by black fungus powder, carries out boiling, pressure 0.05-1.05 under high pressure
MPa, high-voltage time are 40-60 min；
9）The filtering refers to the black fungus liquid of autoclaving, is handled by vacuum filtration, leave and take filtrate；
10）The evaporation and concentration refers to that filtered filtrate is concentrated, and the volume after concentration is the 40- of initial volume
55%;
11）The described collection precipitation, refers to the ethanol solution for being added 95%, places 12 hours overnight under the conditions of 4 DEG C, later 4
20 min are centrifuged under the conditions of DEG C collects precipitation, i.e. Blackfungus polyhexose crude extract；
12）The purifying drying, refers to Blackfungus polyhexose crude extract, is handled using Sevage solution purifications after adding water to redissolve,
Again through vacuum freeze drying, wherein feed liquid thickness is 3 mm, and in -80 DEG C of refrigerators, the pre-freeze time is 8-15 h, and freeze-drying time is
20-28 h, last arrives Blackfungus polyhexose.
2. a kind of oyster Semen Maydis polypeptide electuary of anti-fatigue effect, which is characterized in that major ingredient is according to weight proportion, including 85-92%
Mixed polypeptide powder, 8-15% Blackfungus polyhexoses.
3. a kind of oyster Semen Maydis polypeptide electuary of anti-fatigue effect according to claim 2, which is characterized in that auxiliary material according to
Weight proportion, including 80-85% skimmed milk powders, 8-12% taurines, 1-2% zinc gluconates, 3-6% vitamin Es, 1-4% lemons
Acid.
4. a kind of oyster Semen Maydis polypeptide electuary of anti-fatigue effect according to claim 3, which is characterized in that every 100 g
5-10 g auxiliary materials are added in major ingredient.
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| CN112753922A (en) * | 2021-01-25 | 2021-05-07 | 北京大学第三医院（北京大学第三临床医学院） | Solid sports beverage for promoting recovery and preparation method thereof |
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