CN104975061A

CN104975061A - Preparation method of camellia seeds

Info

Publication number: CN104975061A
Application number: CN201510488196.8A
Authority: CN
Inventors: 邓小玲; 宁发子; 宁成刚
Original assignee: Dou Hao Bio Tech Ltd Chongqing
Current assignee: Dou Hao Bio Tech Ltd Chongqing
Priority date: 2015-08-11
Filing date: 2015-08-11
Publication date: 2015-10-14

Abstract

The invention discloses a preparation method of camellia seeds, belonging to the technical field of application of a biotechnology in a food industry. According to the preparation method, waste byproduct camellia seed meals produced by processing camellia seeds are used as raw materials, the camellia seed meals are crushed and dissolved, then are subjected to enzymolysis, enzyme deactivation, centrifugation, membrane separation and purification, concentration and spray drying, and thus a protein small peptide finished product is obtained. In the obtained product protein, the total nitrogen content is 15% or more, and the content of small peptide is more than or equal to 90%. The prepared camellia seeds can be applied to the fields of foods, medicines, health products and the like.

Description

The preparation method of the little peptide of a kind of tea seed

Technical field

The invention belongs to food processing field, particularly relate to the preparation method of the little peptide of a kind of tea seed.

Background technology

The current whole nation produces tea seed about 1,060,000 tons every year, and process for processing oil tea oil about 27.7 ten thousand tons, the tea seed grouts after prepressing extraction are at about 700,000 tons.Tea seed grouts are protein resources of high-quality, and protein content accounts for 15-17%, and amino acid consists of 17 kinds, and wherein 8 kinds is essential amino acid, may be used for the production of the multiple products such as medicine, food, feed.Therefore, take camellia seed meal as protein raw materials, under biological enzyme condition, hydrolysis generates the material such as polypeptide and amino acid, and have very high nutritive value and functional performance, this method becomes study hotspot.The polypeptide produced after a variety of plant protein enzymolysis except there is easily digested characteristic, also have very strong anti-oxidant, reduce the functional performances such as cholesterol.Tea seed grouts are used as feed or fertilizer more, and not yet fully deep processing and utilization, thus causes the significant wastage of tea seed protein resource.If carry out limited enzymolysis to tea seed albumen, can produce high nutrition, high added value and be easy to absorb the biological products with bioactive functions.Carry out and the research of tea seed little peptide nourishing function activity is had important practical significance.

The little peptide of tea seed refers to that tea seed albumen is after proteolytic enzyme effect, then through the protein hydrolyzate that process obtains such as being separated, refining.Its total nitrogen all reaches 15 more than %, and molecular weight is below 2000, water-soluble all at 90 more than %.Research proves, tea seed albumen is the mixture of multiple little polypeptide through the product of composite protease hydrolysis, its molecular weight is substantially in 300 ~ 2000 scopes, its middle-molecular-weihydroxyethyl accounts for 98.5 % below 1000, this part component is made up of the little peptide below 8 polypeptide, and many functional little peptides focus mostly in this section.

The little peptide of tea seed has good functional performance, and polypeptide solution as little in tea seed in higher concentrations viscosity is still lower, can keep dissolved state within the scope of wider pH value; There is higher water absorbability and moisture retention; Osmotic pressure is more much lower than amino acid; Arrestin matter can form gel, decrease the allergenicity because tea seed protein macromolecule causes, add the stability etc. of product.

The reaction conditions of enzyme hydrolysis method is gentle, and consuming little energy, amino acid is not destroyed, and efficiency is high, and the reservation of nutritive ingredient has incomparable advantage, has wide market outlook.

The object of this invention is to provide a kind of method of green, the little peptide of efficient, safe tea seed, reaction conditions is gentle, reaction times is short, method is simple, workable, and output capacity is high, nutritive ingredient activeconstituents retains completely, and solve a difficult problem for tea seed by-product utilization, expand Application Areas, with low cost and remarkable in economical benefits.

Summary of the invention

Technical problem to be solved by this invention is to provide the production technique of the little Gly-His-Lys of a kind of tea seed, can, by the various nutritive ingredient degradeds in camellia seed meal, make it generate that directly can absorb for human body, the nutritious little Gly-His-Lys of tea seed.

For solving the problems of the technologies described above the technical solution adopted in the present invention be:

A preparation method for the little peptide of tea seed, this preparation method comprises the following steps:

1, pulverize: camellia seed meal is pulverized, as tea seed small peptide production raw material after mistake 80 eye mesh screens;

2, dissolve: the quality of camellia seed meal and deionized water and volume ratio are: 1:10 ~ 1:20, stirring and dissolving, and temperature is 45 DEG C, and the time is 30min, obtain tea seed protein powder suspension I;

3, enzymolysis: add proteolytic enzyme and carry out enzyme digestion reaction in described filtrate I, obtain enzymolysis solution II;

4, Ultra filtration membrane: by described enzymolysis solution II by Ultra filtration membrane process, obtain filtrate I II;

5, multiple-effect evaporation concentrates: concentrated by multiple-effect evaporator by described filtrate I II, make the solid content in filtrate be promoted to 35 ~ 55%(w/v);

6, dry: by the filtrate I II after concentrated through high-pressure spray-drying process, to obtain the little peptide of described tea seed.

Compared with prior art, the advantage of the inventive method and beneficial effect as follows:

The purity of the little peptide of tea seed that l, employing the inventive method are produced is high, and the content of the tea seed peptide of its gross protein middle-molecular-weihydroxyethyl below 1000 dalton is all more than 98%;

The molecular weight of the tea seed peptide 2, adopting the inventive method to produce is little, and human body easily absorbs, and specific absorption reaches more than 90%:

3, adopt membrane separation technique of the present invention, liquid towards material carries out being separated, concentrating and purifying, grasps all at normal temperatures

Do, without phase-state change, energy-efficient, and do not produce pollution in production process;

4, the inventive method with short production cycle, cost is low, reaction temperature and, easy and simple to handle, green non-pollution, safe without toxic side effect, the fields such as food, medicine, healthcare products can be widely used in.

Embodiment

Below by specific embodiment, the inventive method is described in further detail.

Embodiment 1

An extracting method for tea seed peptide, step is as follows:

Get the camellia seed meal of 10 kilogram protein content more than 50%, pulverized 80 eye mesh screens, be dissolved into and fill in 100 kilograms of deionized waters, 45 DEG C are stirred 30min, then add compound protease 250 grams, at PH8.5, react 8 hours in the water bath with thermostatic control vibrator of 50 DEG C; Heat up in 90 DEG C of water-baths and keep 5 minutes, make enzyme deactivation; Cold water is cooled to room temperature, and the concentrated and three-effect evaporation and condensation through ultra-filtration membrane, spraying dry obtains the little peptide of tea seed 13.2 kilograms.Peptide protein total nitrogen 15.2%, gross protein Small Peptides (molecular weight is below 1000 dalton) content 98.4%, ash content 5.3%.

Embodiment 2:

Get the camellia seed meal of 20 kilogram protein content more than 50%, pulverized 80 eye mesh screens, be dissolved into and fill in 300 kilograms of deionized waters, 45 DEG C are stirred 30min, add compound protease 550 grams again, at PH9.0, react 10 hours in the water bath with thermostatic control vibrator of 55 DEG C; Heat up in 90 DEG C of water-baths and keep 5 minutes, make enzyme deactivation; Cold water is cooled to room temperature, and the concentrated and three-effect evaporation and condensation through ultra-filtration membrane, spraying dry obtains the little peptide of tea seed 10.4 jin.After testing: little peptide protein total nitrogen 15.6%, gross protein Small Peptides (molecular weight is below 1000 dalton) content 98.8%, ash content 4.2%.

The little peptide detected result of tea seed

Test item	National standard	Detected result
			Form	Powdered, without caking phenomenon	Powdered, without caking
Color and luster	Yellow or light yellow	Light yellow
			Flavour and smell	The flavour having product to have and smell	Nothing
Impurity	Without the visible tramp material of twenty-twenty vision	Inclusion-free
			Gross protein	≥80.0%	96.5%
Peptide (molecular weight is below 1000 dalton) content in gross protein	≥75.0%	98.5%
			PH value (the lOwt% aqueous solution)	7.0-8.0	7.5
Moisture	≤7.0%	3.2%
			Ash content	≤10.0%	6.2%
Arsenic content (in As element)	≤0.5mg/kg	0.2mg/kg
			Lead content (in Pb element)	≤0.5mg/kg	0.2mg/kg
Total plate count	≤30000cfu/g	1500cfu/g
			Coliform	≤40MPN/lOOg	<20MPN/lOOg
Molds and yeasts	≤50cfu/g	<lOcfu/g
			Pathogenic bacterium	Must not detect	Do not detect

Claims

1. a preparation method for the little peptide of tea seed, is characterized in that, comprise step:

A () pulverizes: pulverized by camellia seed meal, as tea seed small peptide production raw material after mistake 80 eye mesh screens;

B () dissolves: the quality of camellia seed meal and deionized water and volume ratio are: 1:10 ~ 1:20, stirring and dissolving, and temperature is 45 DEG C, and the time is 30min, obtain tea seed protein powder suspension I;

(c) enzymolysis: add proteolytic enzyme and carry out enzyme digestion reaction in described filtrate I, obtain enzymolysis solution II;

(d) Ultra filtration membrane: by described enzymolysis solution II by Ultra filtration membrane process, obtain filtrate I II;

E () is dry: described filtrate I II is dry, obtains the little peptide of described tea seed.

2. preparation method as claimed in claim 1, it is characterized in that, in described step (c), proteolytic enzyme is compound protease, the addition of described compound protease is 0.1 ~ 0.3%(w/v of described filtrate I volume), hydrolysis temperature is 45 ~ 60 DEG C, PH7 ~ 9, enzymolysis time 6 ~ 10 hours.

3. preparation method as claimed in claim 1, is characterized in that, in described step (d), the concrete grammar of Ultra filtration membrane is: with the XD-D4-4540 ultrafiltration system of Xinda, Hefei film Science and Technology Ltd., carry out ultrafiltration with the ultra-filtration membrane of 1000Da.

4. preparation method as claimed in claim 1, it is characterized in that, described step (e) comprising:

(e1): concentrated by multiple-effect evaporator by described filtrate I II, the solid content in filtrate is made to be promoted to 35 ~ 55%(w/v);

(e2): by the filtrate I II after concentrated through high-pressure spray-drying process, obtain the little peptide of described tea seed.

5. the little peptide of the tea seed that the preparation method as described in any one of Claims 1 to 4 obtains.

6. the little peptide of tea seed as claimed in claim 5, is characterized in that, described tea seed little peptide finished product look yellowish, have the distinctive fragrance of tea seed, and molecular weight is within 1000Da.

7. a purposes for the little peptide of tea seed as claimed in claim 6, is characterized in that, can be widely used in the fields such as food, medicine, healthcare products.