CN100469256C - Method for preparing yeast peptide utilizing beer yeast - Google Patents

Abstract

The present invention discloses a preparation method capable of utilizing beer yeast produced in the beer production to make yeast peptide. Said method includes the following step: collecting beer yeast, extruding, mixing paste, zymolysis and breaking wall, secondary mixing paste, zymolyzing protein, concentrating, drying, pulverizing so as to obtain the invented yeast peptide. Said yeast peptide can be used as raw material for making multifunctional health-care product.

Description

A kind of preparation method who utilizes brewer's yeast to make the yeast peptide

Technical field

The present invention relates to the preparation method of a primary yeast peptide, specifically is a kind of preparation method who utilizes brewer's yeast to make the yeast peptide, belongs to food processing field.

Background technology

China's brewing industry development is swift and violent, and beer production was sure to occupy the first in the world in continuous 4 years, and 2005 above 3,000 ten thousand tons.Continuous growth along with beer production, various discarded objects also increase sharply thereupon in the production process, wherein Zui Da discarded object is exactly a brewer's yeast, the generation of brewer's yeast accounts for about 8 ‰ of beer production, brewer's yeast is nutritious, be by 35%～50% protein, other materials such as 35%～40% carbohydrate and nucleic acid are formed, it is a kind of good single cell protein resource, and contain 8 essential seed amino acids of humans and animals body and 14 kinds of mineral matters, the vitamin of almost all kinds, nutriment such as nucleic acid and glutathione, have and be good for the stomach, laxation, strengthen liver function, prevention hypertension, diabetes and the anti-ageing multiple efficacies of waiting for a long time.But the beer yeast cells wall is very thick, is difficult to directly digest and assimilate.Yeast extract after the dissolving has strong meat fragrance, also have a large amount of little peptides simultaneously and be the nutriment that flavor amino acid, vitamin etc. can directly absorb, therefore, since the nineties in last century, constantly study employing interpolation enzyme preparation at food processing field and carry out broken wall and degraded yeast protein, will produce yeast extract after the Saccharomyces mycetolysis.Because yeast extract contains physiological activators such as active trophic factors, the anti-ageing factor such as abundant glutathione, guanosine, inosine and glucan, mannosan, therefore in medicine and food, begun to have more application, but practical application all is as raw material with other yeast such as Saccharomyces cerevisiaes, utilize brewer's yeast production yeast extract and amino acid but seldom, its reason mainly is because the bitter taste of brewer's yeast and special yeast stink.At present, most of brewery can only be brewer's yeast oven dry back as feed, and not only added value is very low, and owing to the problem of beer yeast cells wall thickness is not resolved, so not only be difficult to digest and absorb, and after excreting, also can cause the pollution of environment.In recent years, in food, medicine and feedstuff industry, utilize fish meal or soybean protein to enter the stage of industrialization, and utilize brewer's yeast production Functional Polypeptides to yet there are no report for raw material production Functional Polypeptides product.

Summary of the invention

The objective of the invention is to overcome the defective of similar technology, disclose a kind of preparation method who utilizes brewer's yeast to make the yeast peptide, it is the biological characteristics according to yeast, utilizes physics and biotechnology to be processed into the yeast peptide.

The present invention finishes by the following steps preparation:

Collect brewer's yeast → extruding spray quick-fried → size mixing → enzymolysis broken wall → secondary sizes mixing → enzymolysis protein → concentrated → drying → pulverizing → yeast peptide.

The present invention has thoroughly solved the defective of other similar technology, its technology is simple, the product form ideal, the yeast peptide that utilizes the present invention to prepare has kept original nutrition of brewer's yeast and functional component well, and by expanded and enzymolysis, improved the content of yeast peptide greatly, wherein soluble protein content has improved 50%, molecular weight accounts for 75% of total protein at the peptide content below 10,000, wherein glutathione content reaches 2%, also contain a certain amount of glucan and mannosan, product is is not only digested and assimilated easily, has also strengthened specific function.

The specific embodiment

Concrete operations step of the present invention is as follows:

A. collect beer yeast slurry or dusty yeast,

B. the extruding spray is quick-fried

B.1 twin-screw extruding

B.2 rate of feeding is 30-35kg/h

B.3 screw speed is 240-250r/min

B.4 extrusion temperature is 85 ℃-95 ℃,

C. size mixing

C.1 make solid content reach 50%-55%

C.2 transfer pH to 4.5-4.8,

D. enzymolysis broken wall

D.1 add wall breaking enzyme 0.3%-0.5%

D.2 hydrolysis temperature is 50 ℃-60 ℃

D.3 enzymolysis time 13h-15h,

E. secondary is sized mixing

E.1 transfer pH to 7.5-7.8,

F. enzymolysis protein

F.1 add proteinase-10 .1%-0.2%

F.2 hydrolysis temperature is 40 ℃-50 ℃

F.3 enzymolysis time 23h-25h,

G. concentrate

G.1 membrane filtration

G.2 adopt triple effect evaporator to concentrate solid content and reach 75%-80%,

H. dry

H.1 select pneumatic conveying drying for use, or

H.2 microwave equipment drying

H.2.1 temperature is 95 ℃-100 ℃

H.2.2 time 8min-12min,

I. pulverize,

J. yeast peptide.

The present invention can be made into full powder, also can make the particle of different shape through the die forming drying, and product can be used in food, medicine or feedstuff industry respectively as the functional health product raw material according to its sanitation degree difference.The present invention is easy to operate, continuity good, treating capacity is big, easily realize industrialization processing, products obtained therefrom steady quality, safety, substantially solved the discarded reluctant technology barrier of brewer's yeast of brewery, change the present situation of Functional Polypeptides product rareness in the market, realized the increment of brewer's yeast, and driven the development of Functional Polypeptides related industry, form new growth engines, have the huge economic benefit and the prospect of marketing.

Claims (1)

1. preparation method who utilizes brewer's yeast to make the yeast peptide is characterized in that finishing by following operating procedure:

1.a collect beer yeast slurry or dusty yeast;

1.b the extruding spray is quick-fried

1.b.1 twin-screw extruding

1.b.2 rate of feeding is 30-35kg/h

1.b.3 screw speed is 240-250r/min

1.b.4 extrusion temperature is 85 ℃-95 ℃;

1.c size mixing

1.c.1 make solid content reach 50%-55%

1.c.2 transfer pH to 4.5-4.8;

1.d enzymolysis broken wall

1.d.1 add wall breaking enzyme 0.3%-0.5%

1.d.2 50 ℃-60 ℃ of hydrolysis temperatures

1.d.3 enzymolysis time 13h-15h;

1.e secondary is sized mixing

1.e.1 transfer pH to 7.5-7.8;

1.f enzymolysis protein

1.f.1 add proteinase-10 .1%-0.2%

1.f.2 40 ℃-50 ℃ of hydrolysis temperatures

1.f.3 enzymolysis time 23h-25h;

1.g concentrate

1.g.1 membrane filtration

Reach 75%-80% 1.g.2 adopt triple effect evaporator to concentrate solid content;

1.h it is dry

1.h.1 select pneumatic conveying drying for use, or

1.h.2 microwave equipment drying

1.h.2.1 95 ℃-100 ℃ of temperature

1.h.2.2 time 8min-12min;

1.i pulverize;

1.j yeast peptide.