CN105581345A - Preparation method for glutathione-enriched nucleic acid yeast powder - Google Patents
Preparation method for glutathione-enriched nucleic acid yeast powder Download PDFInfo
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- CN105581345A CN105581345A CN201510969541.XA CN201510969541A CN105581345A CN 105581345 A CN105581345 A CN 105581345A CN 201510969541 A CN201510969541 A CN 201510969541A CN 105581345 A CN105581345 A CN 105581345A
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- mother liquor
- glutathione
- nucleic acid
- enzymolysis
- yeast
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- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 title claims abstract description 86
- 240000004808 Saccharomyces cerevisiae Species 0.000 title claims abstract description 59
- 229960003180 glutathione Drugs 0.000 title claims abstract description 52
- 108010024636 Glutathione Proteins 0.000 title claims abstract description 50
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 32
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 32
- 239000000843 powder Substances 0.000 title claims abstract description 27
- 238000002360 preparation method Methods 0.000 title claims abstract description 19
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 14
- 239000012452 mother liquor Substances 0.000 claims abstract description 54
- 238000003756 stirring Methods 0.000 claims description 20
- -1 glutathione nucleic acid Chemical class 0.000 claims description 18
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 15
- 238000000034 method Methods 0.000 claims description 15
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 8
- 239000004365 Protease Substances 0.000 claims description 8
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 8
- RQPZNWPYLFFXCP-UHFFFAOYSA-L barium dihydroxide Chemical compound [OH-].[OH-].[Ba+2] RQPZNWPYLFFXCP-UHFFFAOYSA-L 0.000 claims description 8
- 229910001863 barium hydroxide Inorganic materials 0.000 claims description 8
- 230000007423 decrease Effects 0.000 claims description 8
- 239000000284 extract Substances 0.000 claims description 8
- 229910052708 sodium Inorganic materials 0.000 claims description 8
- 239000011734 sodium Substances 0.000 claims description 8
- 238000010792 warming Methods 0.000 claims description 8
- 239000002773 nucleotide Substances 0.000 claims description 6
- 125000003729 nucleotide group Chemical group 0.000 claims description 6
- 239000012535 impurity Substances 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 108090000526 Papain Proteins 0.000 claims description 4
- 108091005804 Peptidases Proteins 0.000 claims description 4
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 4
- 239000003513 alkali Substances 0.000 claims description 4
- 238000006243 chemical reaction Methods 0.000 claims description 4
- 230000006835 compression Effects 0.000 claims description 4
- 238000007906 compression Methods 0.000 claims description 4
- 230000000694 effects Effects 0.000 claims description 4
- 239000000796 flavoring agent Chemical class 0.000 claims description 4
- 235000019634 flavors Nutrition 0.000 claims description 4
- 239000013505 freshwater Substances 0.000 claims description 4
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 4
- 230000000937 inactivator Effects 0.000 claims description 4
- 235000019834 papain Nutrition 0.000 claims description 4
- 229940055729 papain Drugs 0.000 claims description 4
- 235000019419 proteases Nutrition 0.000 claims description 4
- 238000004062 sedimentation Methods 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 4
- 238000000967 suction filtration Methods 0.000 claims description 4
- 239000006228 supernatant Substances 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 12
- 150000001413 amino acids Chemical class 0.000 abstract description 8
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 6
- 239000002699 waste material Substances 0.000 abstract description 5
- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 4
- 229920001184 polypeptide Polymers 0.000 abstract description 3
- 102000004196 processed proteins & peptides Human genes 0.000 abstract description 3
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- 208000035404 Autolysis Diseases 0.000 abstract 3
- 206010057248 Cell death Diseases 0.000 abstract 3
- 230000028043 self proteolysis Effects 0.000 abstract 3
- 238000001035 drying Methods 0.000 abstract 1
- 239000004615 ingredient Substances 0.000 abstract 1
- 238000001694 spray drying Methods 0.000 abstract 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 42
- 230000000050 nutritive effect Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000005667 attractant Substances 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000008429 bread Nutrition 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 230000031902 chemoattractant activity Effects 0.000 description 1
- BERDEBHAJNAUOM-UHFFFAOYSA-N copper(I) oxide Inorganic materials [Cu]O[Cu] BERDEBHAJNAUOM-UHFFFAOYSA-N 0.000 description 1
- KRFJLUBVMFXRPN-UHFFFAOYSA-N cuprous oxide Chemical compound [O-2].[Cu+].[Cu+] KRFJLUBVMFXRPN-UHFFFAOYSA-N 0.000 description 1
- 229940112669 cuprous oxide Drugs 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 235000003969 glutathione Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000007634 remodeling Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
- C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
- C07K5/08—Tripeptides
- C07K5/0819—Tripeptides with the first amino acid being acidic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- General Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Botany (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention relates to a preparation method for nucleic acid yeast powder. The yeast powder is rich in glutathione and is obtained through the steps that mother liquor obtained after glutathione is extracted is pretreated and then mixed with fresh yeast, and enzymolysis and drying are performed. The preparation method of the yeast autolysis powder comprises the following steps that 1, the mother liquor obtained after glutathione is extracted is pretreated; 2, the mother liquor is settled; 3, primary enzymolysis is performed; 4, secondary enzymolysis is performed; 5, after secondary enzymolysis is finished, the glutathione-enriched nucleic acid yeast autolysis powder is obtained through spray drying. The preparation method has the advantages that the mother liquor obtained after glutathione is extracted is recycled in a reasonable mode, the waste material is changed into things of value, and waste of glutathione and the amino acid polypeptide ingredients is avoided; the obtained autolysis powder is high in content of total nitrogen and amino acid nitrogen, high in nutritional value, low in ash content and good in product quality.
Description
Technical field
The present invention relates to a kind of preparation method of yeast derivative, relate to specifically a kind of preparation method of rich glutathione nucleic acid yeast powder.
Background technology
Yeast is widely used in the field such as bread fermentation, beer fermentation, yeast of a great variety, comprises Saccharomyces cerevisiae, brewer's yeast, bakers' yeast, contains the material such as rich in protein, amino acid in yeast, by to yeast hydrolysis, can obtain the material such as protein or amino acid of yeast.
It is raw material that autolysing yeast powder is selected special yeast cake, adopt the new and high technologies such as high-efficiency wall breaking and multi-joint enzymolysis, form through purification, product is rich in nucleotides, the multiple nutritional components such as the little peptide of nutrition, glutathione and yeast cell wall polysaccharide, containing carrier, high temperature resistant, can granulate, animal is had to splendid attractant, immunity and growth promotion. Be applicable to aquatic products, livestock and poultry and feed for pet.
The rich glutathione yeast acquisition glutathione that is used for fermenting, the extracting method that traditional cost is minimum is mantoquita method, the yeast extract that contains GSH reacts with cuprous oxide and generates precipitation GSCu, then reduction obtains GSH, but in the mother liquor after removal precipitation, still contain a large amount of glutathione and amino acid, polypeptide, and in prior art, mother liquor is mostly discharged into treatment tank and does wastewater treatment, does not reclaim comprehensive utilization.
Summary of the invention
The object of the invention is to solve existing deficiency, a kind of preparation method of rich glutathione nucleic acid yeast powder is provided, glutathione is extracted to mother liquor turns waste into wealth, and obtains the nucleic acid yeast powder that glutathione content is high, be of high nutritive value.
The technical solution adopted for the present invention to solve the technical problems is:
A kind of preparation method of rich glutathione nucleic acid yeast powder, described dusty yeast is rich in glutathione and flavour nucleotide, by the mother liquor process pretreatment of extracting after glutathione, then mix with fresh nucleic acid yeast, through enzymolysis, dry acquisition, the preparation method of described rich glutathione nucleic acid yeast powder carries out as follows:
(1) glutathione extracts mother liquor pretreatment: press 0.5-0.8Kg/ ton mother liquor and add vulcanized sodium, in stirring at normal temperature reaction 10-15min, then adjust mother liquor pH to 5.6-5.8 with barium hydroxide, stir 30-45min, ensure that barium hydroxide fully reacts with mother liquor, fully remove the impurity in mother liquor;
(2) mother liquor sedimentation: more than the completely reacted mother liquor of step (1) is delivered to subsider natural subsidence 20-24h, then supernatant or suction filtration or plate compression, fully removes impurity stand-by;
(3) collect the nucleic acid yeast mud of fresh water capacity 40-50%, add the mother liquor of step (2), be warming up to 55-60 DEG C, stir, then adopt high pressure homogenizer homogeneous 1-2 time;
(4) primary enzymolysis, yeast juice after high-pressure homogeneous is adjusted pH to 7.20-7.5 with NaOH, then adjusting temperature is 50-55 DEG C, the alkali protease that by volume mark 10-12 ‰ adds 10-20 ten thousand units carries out primary enzymolysis, stir rear beginning timing enzymolysis 5-8 hour, process temperature maintains 50-55 DEG C, and pH allows it naturally decline, when pH during to 6.0-6.1 left and right primary enzymolysis finish;
(5) secondary enzymolysis: after primary enzymolysis, adjust pH to 5.4 with dilute sulfuric acid, temperature 50-55 DEG C, then by volume mark 0.8-1 ‰ adds the papain of 80-100 ten thousand units, enzymolysis 0.5-1.5 hour, process pH allows it naturally decline;
(6) dry: after secondary enzymolysis finishes, to be warming up to 80-82 DEG C, to maintain 5-10min inactivator, then the rich glutathione nucleic acid yeast of spray-dried acquisition powder.
The pH value that described step (1) GSH-PX activity extracts mother liquor is 1.8-2.5, glutathione content 0.5-0.6g/L, and solid quality content is 5-5.5%.
In step (1), the existing water of vulcanized sodium dissolves, and the mode then adding with stream adds to be treated in pretreated mother liquor, and limit stream edged stirs.
In step (3), the mass ratio of mother liquor and yeast paste is 1:1.
The invention has the beneficial effects as follows: (1) reclaims the mother liquor extracting after glutathione by reasonable manner, turn waste into wealth, avoided glutathione in mother liquor and the waste of amino acid polypeptide composition, reduced cost of sewage disposal; (2) adopt rational enzymolysis process, the nucleic acid yeast powder nucleotide content of acquisition, total nitrogen, amino acid nitrogen content are high, are of high nutritive value, and ash content is low, superior product quality.
Detailed description of the invention
Below by specific embodiment, technical scheme of the present invention is described in further detail.
Embodiment 1:
A kind of preparation method of rich glutathione nucleic acid yeast powder, described dusty yeast is rich in glutathione and flavour nucleotide, by the mother liquor process pretreatment of extracting after glutathione, then mix with fresh nucleic acid yeast, through enzymolysis, dry acquisition, the preparation method of described rich glutathione nucleic acid yeast powder carries out as follows:
(1) glutathione extracts mother liquor pretreatment: press 0.5Kg/ ton mother liquor and add vulcanized sodium, in stirring at normal temperature reaction 10min, then adjust mother liquor pH to 5.6 with barium hydroxide, stir 30min, ensure that barium hydroxide fully reacts with mother liquor;
(2) mother liquor sedimentation: more than the completely reacted mother liquor of step (1) is delivered to subsider natural subsidence 20h, then supernatant or suction filtration or plate compression, fully removes impurity stand-by;
(3) collect the nucleic acid yeast mud of fresh water capacity 40-50%, add the mother liquor of step (2), be warming up to 55 DEG C,, stir, then adopt high pressure homogenizer homogeneous 1 time;
(4) primary enzymolysis, yeast juice after high-pressure homogeneous is adjusted pH to 7.20 with NaOH, then adjusting temperature is 50 DEG C, the alkali protease that by volume mark 10 ‰ adds 100,000 units carries out primary enzymolysis, stir rear beginning timing enzymolysis 5 hours, process temperature maintains 50 DEG C, and pH allows it naturally decline, when pH during to 6.0 left and right primary enzymolysis finish;
(5) secondary enzymolysis: after primary enzymolysis, adjust pH to 5.4 with dilute sulfuric acid, temperature 50 C, then by volume mark 0.8 ‰ adds the papain of 800,000 units, enzymolysis 0.5 hour, process pH allows it naturally decline;
(6) after secondary enzymolysis finishes, be warming up to 80 DEG C, maintain 5min inactivator, then the rich glutathione nucleic acid yeast of spray-dried acquisition powder.
The pH value that described step (1) GSH-PX activity extracts mother liquor is 1.8, glutathione content 0.5g/L, and solid quality content is 5%.
In step (1), the existing water of vulcanized sodium dissolves, and the mode then adding with stream adds to be treated in pretreated mother liquor, and limit stream edged stirs.
In step (3), the mass ratio of mother liquor and yeast paste is 1:1.
Embodiment 2:
A kind of preparation method of rich glutathione nucleic acid yeast powder, described dusty yeast is rich in glutathione and flavour nucleotide, by the mother liquor process pretreatment of extracting after glutathione, then mix with fresh nucleic acid yeast, through enzymolysis, dry acquisition, the preparation method of described rich glutathione nucleic acid yeast powder carries out as follows:
(1) glutathione extracts mother liquor pretreatment: press 0.8Kg/ ton mother liquor and add vulcanized sodium, in stirring at normal temperature reaction 15min, then adjust mother liquor pH to 5.8 with barium hydroxide, stir 45min, ensure that barium hydroxide fully reacts with mother liquor;
(2) mother liquor sedimentation: more than the completely reacted mother liquor of step (1) is delivered to subsider natural subsidence 24h, then supernatant or suction filtration or plate compression, fully removes impurity stand-by;
(3) collect the nucleic acid yeast mud of fresh water capacity 40-50%, add the mother liquor of step (2), be warming up to 60 DEG C, stir, then adopt high pressure homogenizer homogeneous 2 times;
(4) primary enzymolysis, yeast juice after high-pressure homogeneous is adjusted and is stirred with NaOH, then adjust pH to 7.5 with NaOH, then adjusting temperature is 55 DEG C, the alkali protease that by volume mark 12 ‰ adds 200,000 units carries out primary enzymolysis, stirs rear beginning timing enzymolysis 8 hours, and process temperature maintains 55 DEG C, pH allows it naturally decline, when pH during to 6.1 left and right primary enzymolysis finish;
(5) secondary enzymolysis: after primary enzymolysis, adjust pH to 5.4 with dilute sulfuric acid, 55 DEG C of temperature, then by volume mark 1 ‰ adds the papain of 1,000,000 units, enzymolysis 1.5 hours, process pH allows it naturally decline;
(6) after secondary enzymolysis finishes, be warming up to 82 DEG C, maintain 10min inactivator, then the rich glutathione nucleic acid yeast of spray-dried acquisition powder.
The pH value that described step (1) GSH-PX activity extracts mother liquor is 2.5, glutathione content 0.6g/L, and solid quality content is 5.5%.
In step (1), the existing water of vulcanized sodium dissolves, and the mode then adding with stream adds to be treated in pretreated mother liquor, and limit stream edged stirs.
In step (3), the mass ratio of mother liquor and yeast paste is 1:1.
The quality index of the dusty yeast being of high nutritive value that embodiment 1-2 prepares is as follows:
| Embodiment | Glutathione content | Total nitrogen content | Nucleic acid content | Amino acid nitrogen content | Ash content |
| 1 | 1.05% | 10.8% | 5.8% | 5.2% | 0.98% |
| 2 | 1.12% | 12.5% | 6.5% | 5.5% | 1.2% |
Above-described embodiment is preferably scheme of one of the present invention, not the present invention is done to any pro forma restriction, also has other variant and remodeling under the prerequisite that does not exceed the technical scheme that claim records.
Claims (4)
1. the preparation method of a rich glutathione nucleic acid yeast powder, it is characterized in that: described dusty yeast is rich in glutathione and flavour nucleotide, by the mother liquor process pretreatment of extracting after glutathione, then mix with fresh nucleic acid yeast, through enzymolysis, dry acquisition, the preparation method of described rich glutathione nucleic acid yeast powder carries out as follows:
(1) glutathione extracts mother liquor pretreatment: press 0.5-0.8Kg/ ton mother liquor and add vulcanized sodium, in stirring at normal temperature reaction 10-15min, then adjust mother liquor pH to 5.6-5.8 with barium hydroxide, stir 30-45min, ensure that barium hydroxide fully reacts with mother liquor;
(2) mother liquor sedimentation: more than the completely reacted mother liquor of step (1) is delivered to subsider natural subsidence 20-24h, then supernatant or suction filtration or plate compression, fully removes impurity stand-by;
(3) collect the nucleic acid yeast mud of fresh water capacity 40-50%, add the mother liquor of step (2), be warming up to 55-60 DEG C, stir, then adopt high pressure homogenizer homogeneous 1-2 time;
(4) primary enzymolysis, yeast juice after high-pressure homogeneous is adjusted pH to 7.20-7.5 with NaOH, then adjusting temperature is 50-55 DEG C, the alkali protease that by volume mark 10-12 ‰ adds 10-20 ten thousand units carries out primary enzymolysis, stir rear beginning timing enzymolysis 5-8 hour, process temperature maintains 50-55 DEG C, and pH allows it naturally decline, when pH during to 6.0-6.1 left and right primary enzymolysis finish;
(5) secondary enzymolysis: after primary enzymolysis, adjust pH to 5.4 with dilute sulfuric acid, temperature 50-55 DEG C, then by volume mark 0.8-1 ‰ adds the papain of 80-100 ten thousand units, enzymolysis 0.5-1.5 hour, process pH allows it naturally decline;
(6) dry: after secondary enzymolysis finishes, to be warming up to 80-82 DEG C, to maintain 5-10min inactivator, then the rich glutathione nucleic acid yeast of spray-dried acquisition powder.
2. the preparation method of rich glutathione nucleic acid yeast powder according to claim 1, is characterized in that: the pH value that described step (1) GSH-PX activity extracts mother liquor is 1.8-2.5, glutathione content 0.5-0.6g/L, and solid quality content is 5-5.5%.
3. the preparation method of rich glutathione nucleic acid yeast powder according to claim 1, is characterized in that: in step (1), the existing water of vulcanized sodium dissolves, and the mode then adding with stream adds to be treated in pretreated mother liquor, and limit stream edged stirs.
4. the preparation method of rich glutathione nucleic acid yeast powder according to claim 1, is characterized in that: in step (3), the mass ratio of mother liquor and yeast paste is 1:1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510969541.XA CN105581345A (en) | 2015-12-22 | 2015-12-22 | Preparation method for glutathione-enriched nucleic acid yeast powder |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510969541.XA CN105581345A (en) | 2015-12-22 | 2015-12-22 | Preparation method for glutathione-enriched nucleic acid yeast powder |
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| Publication Number | Publication Date |
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| CN105581345A true CN105581345A (en) | 2016-05-18 |
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| CN201510969541.XA Pending CN105581345A (en) | 2015-12-22 | 2015-12-22 | Preparation method for glutathione-enriched nucleic acid yeast powder |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110150660A (en) * | 2017-12-25 | 2019-08-23 | 江南大学（如皋）食品生物技术研究所 | Selenium-rich nutritive strengthened dose and preparation method thereof |
| CN112911945A (en) * | 2018-10-25 | 2021-06-04 | 联合利华知识产权控股有限公司 | Preparation method of flavoring agent |
| CN114276942A (en) * | 2021-12-30 | 2022-04-05 | 安琪酵母股份有限公司 | Glutathione yeast, preparation method and application of glutathione yeast product |
-
2015
- 2015-12-22 CN CN201510969541.XA patent/CN105581345A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110150660A (en) * | 2017-12-25 | 2019-08-23 | 江南大学（如皋）食品生物技术研究所 | Selenium-rich nutritive strengthened dose and preparation method thereof |
| CN112911945A (en) * | 2018-10-25 | 2021-06-04 | 联合利华知识产权控股有限公司 | Preparation method of flavoring agent |
| CN114276942A (en) * | 2021-12-30 | 2022-04-05 | 安琪酵母股份有限公司 | Glutathione yeast, preparation method and application of glutathione yeast product |
| CN114276942B (en) * | 2021-12-30 | 2024-05-28 | 安琪酵母股份有限公司 | Glutathione yeast, preparation method and application of product |
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Application publication date: 20160518 |