CN102887732A - Method for producing amino acid liquid fertilizer by processing wastewater via tilapias - Google Patents
Method for producing amino acid liquid fertilizer by processing wastewater via tilapias Download PDFInfo
- Publication number
- CN102887732A CN102887732A CN2012104239991A CN201210423999A CN102887732A CN 102887732 A CN102887732 A CN 102887732A CN 2012104239991 A CN2012104239991 A CN 2012104239991A CN 201210423999 A CN201210423999 A CN 201210423999A CN 102887732 A CN102887732 A CN 102887732A
- Authority
- CN
- China
- Prior art keywords
- amino acid
- aspergillus oryzae
- liquid fertilizer
- acid liquid
- tilapia
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
Landscapes
- Fertilizers (AREA)
Abstract
The invention discloses a method for producing an amino acid liquid fertilizer by processing wastewater via tilapias, comprising the steps of grinding tilapia wastes into minced fillet by a grinder, mixing the minced fillet with bran according to a proportion of 3:1 to prepare a solid culture medium, sterilizing the culture medium at a temperature of 121 DEG C for 15 min, inoculating 1ml of Aspergillus oryzae spore suspension with the concentration of 1*108 per ml in every 100g of culture medium after cooling, carrying out solid fermentation and culture at a temperature of 37 DEG C for 48h to obtain culture medium full of Aspergillus oryzae, grinding and adding 100g of grinded yeast into 200ml of protein wastewater, ceaselessly stirring at a temperature of 60 DEG C for 6h, carrying out filtration and 1/3 vacuum concentration and adding calcium and magnesium elements to form the amino acid liquid fertilizer. According to the method, the proteins in the wastewater is decomposed into amino acid by utilizing the protease generated by the Aspergillus oryzae, so that the stink source is eradicated and the influence from the wastewater to the environment is eliminated; and the amino acid liquid fertilizer is produced through blending the calcium and magnesium elements, so that the reclamation of the wastewater is realized.
Description
Technical field
The present invention relates to a kind of a kind of method of tilapia processing waste water resource utilization, especially use the method for the high density proteinic wastewater Preparation of amino acid liquid fertilizer that produces in the tilapia processing.
Background technology
South China four provinces (Guangdong, Hainan, Guangxi, Fujian) tilapia cultivation accounts for nearly 90% of national ultimate production.The aquaculture development impetus is swift and violent, has driven the development of the related industrieies such as seedling, feed, processing, trade, and the tilapia industry has stronger competitive edge in the development of China.Tilapia is except the minority fresh goods is sold, and great majority are processed into freezing Tilapia Fillet and freeze the ground such as the form outlet U.S., Canada, European Union, Japan such as full fish.
The tilapia export processing product of China mainly contains frozen fish fillet and freezes full fish two large classes, be popular in the world market, but the product many places is in the bottom of value of the product chain.Moreover, also exist problems in the processing link, wherein comparatively outstanding is the maimed gurry of protein fish that tilapia is processed into meeting generation about 60% in the Tilapia Fillet, and these wastes will be processed into fish meal.Be processed in the fish meal and can produce a large amount of waste water, these waste water contain 4～6% protein, untimely processing, and waste water is smelly immediately at 1～3 hour, produces the odor pollution environment.Directly discharging, contaminate environment not only, but also be the wasting of resources.
Summary of the invention
The purpose of this invention is to provide a kind of reaction temperature and, cost is low, the amino acid yield the is high tilapia processing waste water method of producing amino-acid liquid fertilizer.
To achieve these goals, technical scheme of the present invention is: a kind of method of producing amino-acid liquid fertilizer with the tilapia processing waste water is provided, may further comprise the steps:
(1) substratum preparation process
Get the maimed gurry of fish after tilapia is processed, pulverize the adult fish gruel with pulverizer, get this fish gruel and wheat bran, ratio by weight 3:1 is fully mixed the two, then this compound is used high pressure steam sterilization 14～16 minutes under 120～130 ℃, be cooled to room temperature and get solid medium, for subsequent use;
(2) inoculation and culturing process
After aspergillus oryzae (Aspergillus oryzae) had been cultivated in the test tube slant, making concentration with sterilized water was 1 * 10
8The aspergillus oryzae spore suspension of individual/ml; Get the solid medium that step (1) obtains, the solid medium inoculation 1ml aspergillus oryzae spore suspension of every 100g; Then under 36～38 ℃, solid fermentation was cultivated 47～49 hours, and is for subsequent use;
(3) enzymolysis process
Get the substratum 100g that step (2) was cultivated 47～49 hours, pulverize, add the proteinic wastewater 200～300ml that produces after the tilapia processing, 60～65 ℃ of insulations, and stir with stirrer, 60 rev/mins of rotating speeds, stirred 5～6 hours, and be heated to 100 ℃, kept 5～6 minutes, then filtered while hot is for subsequent use;
(4) get filtrate 300～350ml that step (3) obtains, vacuum concentration to 200～250ml, according to industry standard NY1429-2010 standard test aminoacids content, aminoacids content should be greater than 100g/L; If aminoacids content is lower than 100g/L, then increases the concentrated ratio of this step, or suitably add through the maimed gurry of the fish of pulverizing at the enzymolysis process of step (3);
(5) through step (4), after amino acid is up to standard, require suitably to add calcium and magnesium elements according to industry standard NY1429-2010, make total concn reach 30g/L;
(6) plastic tank packaging final prod.
The hydrolysis of the present invention and traditional chemical acid system is compared, have reaction temperature and, equipment is simple, invest little, the advantage such as production cost is low; Compare with the industrial enzyme process of direct interpolation, the enzyme that aspergillus oryzae can produce is the prozyme of multiple protein enzyme, and protein hydrolysate is more complete, thereby the amino acid yield is high, need not add the foreign protein enzyme, thereby production cost is lower.The proteolytic enzyme that the present invention utilizes aspergillus oryzae to produce is decomposed into amino acid with the protein in the waste water, has eradicated it and has produced the root of stench, the impact that cancellation waste water causes environment, by suitable allotment calcium and magnesium elements, make and contain amino-acid liquid fertilizer, realized the resource utilization of waste water.
Description of drawings
Being the present invention such as Fig. 1 produces the method process flow diagram of amino-acid liquid fertilizer with the tilapia processing waste water.
Embodiment
A kind of method of producing amino-acid liquid fertilizer with the tilapia processing waste water may further comprise the steps:
(1) substratum preparation process
Get the maimed gurry of fish after tilapia is processed, pulverize the adult fish gruel with pulverizer, get this fish gruel and wheat bran, ratio by weight 3:1 is fully mixed the two, then this compound is used high pressure steam sterilization 14 minutes under 130 ℃, be cooled to room temperature and get solid medium, for subsequent use;
(2) inoculation and culturing process
After aspergillus oryzae (Aspergillus oryzae) had been cultivated in the test tube slant, making concentration with sterilized water was 1 * 10
8The aspergillus oryzae spore suspension of individual/ml; Get the solid medium that step (1) obtains, the solid medium inoculation 1ml aspergillus oryzae spore suspension of every 100g; Then under 36 ℃, solid fermentation was cultivated 49 hours, and is for subsequent use;
(3) enzymolysis process
Get the substratum 100g that step (2) was cultivated 49 hours, pulverize, add the proteinic wastewater 200ml that produces after the tilapia processing, 60 ℃ of insulations, and stir with stirrer, 60 rev/mins of rotating speeds stirred 6 hours, were heated to 100 ℃, kept 5 minutes, then filtered while hot is for subsequent use;
(4) get the filtrate 300ml that step (3) obtains, vacuum concentration is to 200ml, and according to industry standard NY1429-2010 standard test aminoacids content, aminoacids content should be greater than 100g/L; If aminoacids content is lower than 100g/L, then increases the concentrated ratio of this step, or suitably add through the maimed gurry of the fish of pulverizing at the enzymolysis process of step (3);
(5) through step (4), after amino acid is up to standard, require suitably to add calcium and magnesium elements according to industry standard NY1429-2010, make total concn reach 30g/L;
(6) plastic tank packaging final prod.
A kind of method of producing amino-acid liquid fertilizer with the tilapia processing waste water may further comprise the steps:
(1) substratum preparation process
Get the maimed gurry of fish after tilapia is processed, pulverize the adult fish gruel with pulverizer, get this fish gruel and wheat bran, ratio by weight 3:1 is fully mixed the two, then this compound is used high pressure steam sterilization 15 minutes under 121 ℃, be cooled to room temperature and get solid medium, for subsequent use;
(2) inoculation and culturing process
After aspergillus oryzae (Aspergillus oryzae) had been cultivated in the test tube slant, making concentration with sterilized water was 1 * 10
8The aspergillus oryzae spore suspension of individual/ml; Get the solid medium that step (1) obtains, the solid medium inoculation 1ml aspergillus oryzae spore suspension of every 100g; Then under 37 ℃, solid fermentation was cultivated 48 hours, and is for subsequent use;
(3) enzymolysis process
Get the substratum 100g that step (2) was cultivated 48 hours, pulverize, add the proteinic wastewater 200ml that produces after the tilapia processing, 65 ℃ of insulations, and stir with stirrer, 60 rev/mins of rotating speeds stirred 5 hours, were heated to 100 ℃, kept 5 minutes, then filtered while hot is for subsequent use;
(4) get the filtrate 300ml that step (3) obtains, vacuum concentration is to 200ml, and according to industry standard NY1429-2010 standard test aminoacids content, aminoacids content should be greater than 100g/L; If aminoacids content is lower than 100g/L, then increases the concentrated ratio of this step, or suitably add through the maimed gurry of the fish of pulverizing at the enzymolysis process of step (3);
(5) through step (4), after amino acid is up to standard, require suitably to add calcium and magnesium elements according to industry standard NY1429-2010, make total concn reach 30g/L;
(6) plastic tank packaging final prod.
A kind of method of producing amino-acid liquid fertilizer with the tilapia processing waste water may further comprise the steps:
(1) substratum preparation process
Get the maimed gurry of fish after tilapia is processed, pulverize the adult fish gruel with pulverizer, get this fish gruel and wheat bran, ratio by weight 3:1 is fully mixed the two, then this compound is used high pressure steam sterilization 16 minutes under 120 ℃, be cooled to room temperature and get solid medium, for subsequent use;
(2) inoculation and culturing process
After aspergillus oryzae (Aspergillus oryzae) had been cultivated in the test tube slant, making concentration with sterilized water was 1 * 10
8The aspergillus oryzae spore suspension of individual/ml; Get the solid medium that step (1) obtains, the solid medium inoculation 1ml aspergillus oryzae spore suspension of every 100g; Then under 38 ℃, solid fermentation was cultivated 47 hours, and is for subsequent use;
(3) enzymolysis process
Get the substratum 100g that step (2) was cultivated 47 hours, pulverize, add the proteinic wastewater 300ml that produces after the tilapia processing, 65 ℃ of insulations, and stir with stirrer, 60 rev/mins of rotating speeds stirred 5 hours, were heated to 100 ℃, kept 6 minutes, then filtered while hot is for subsequent use;
(4) get the filtrate 350ml that step (3) obtains, vacuum concentration is to 250ml, and according to industry standard NY1429-2010 standard test aminoacids content, aminoacids content should be greater than 100g/L; If aminoacids content is lower than 100g/L, then increases the concentrated ratio of this step, or suitably add through the maimed gurry of the fish of pulverizing at the enzymolysis process of step (3);
(5) through step (4), after amino acid is up to standard, require suitably to add calcium and magnesium elements according to industry standard NY1429-2010, make total concn reach 30g/L;
(6) plastic tank packaging final prod.
Be illustrated in figure 1 as the present invention produces amino-acid liquid fertilizer with the tilapia processing waste water method process flow diagram.
The amino acid production rate of Preparation of amino acid liquid fertilizer of the present invention is that the common enzyme process of 45～52%(is 25%), the finished product aminoacids content is 10～12%, production cost is than common enzyme process low about 20%.
Above disclosed only is preferred embodiment of the present invention, certainly can not limit with this interest field of the present invention, and the equivalent variations of therefore doing according to claim of the present invention still belongs to the scope that the present invention is contained.
Claims (1)
1. method of producing amino-acid liquid fertilizer with the tilapia processing waste water is characterized in that may further comprise the steps:
(1) substratum preparation process
Get the maimed gurry of fish after tilapia is processed, pulverize the adult fish gruel with pulverizer, get this fish gruel and wheat bran, ratio by weight 3:1 is fully mixed the two, then this compound is used high pressure steam sterilization 14～16 minutes under 120～130 ℃, be cooled to room temperature and get solid medium, for subsequent use;
(2) inoculation and culturing process
After aspergillus oryzae had been cultivated in the test tube slant, making concentration with sterilized water was 1 * 10
8The aspergillus oryzae spore suspension of individual/ml; Get the solid medium that step (1) obtains, the solid medium inoculation 1ml aspergillus oryzae spore suspension of every 100g; Then under 36～38 ℃, solid fermentation was cultivated 47～49 hours, and is for subsequent use;
(3) enzymolysis process
Get the substratum 100g that step (2) was cultivated 47～49 hours, pulverize, add the proteinic wastewater 200～300ml that produces after the tilapia processing, 60～65 ℃ of insulations, and stir with stirrer, 60 rev/mins of rotating speeds, stirred 5～6 hours, and be heated to 100 ℃, kept 5～6 minutes, then filtered while hot is for subsequent use;
(4) get filtrate 300～350ml that step (3) obtains, vacuum concentration to 200～250ml, according to industry standard NY1429-2010 standard test aminoacids content, aminoacids content should be greater than 100g/L; If aminoacids content is lower than 100g/L, then increases the concentrated ratio of this step, or suitably add through the maimed gurry of the fish of pulverizing at the enzymolysis process of step (3);
(5) through step (4), after amino acid is up to standard, require suitably to add calcium and magnesium elements according to industry standard NY1429-2010, make total concn reach 30g/L;
(6) plastic tank packaging final prod.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012104239991A CN102887732B (en) | 2012-10-30 | 2012-10-30 | Method for producing amino acid liquid fertilizer by processing wastewater via tilapias |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012104239991A CN102887732B (en) | 2012-10-30 | 2012-10-30 | Method for producing amino acid liquid fertilizer by processing wastewater via tilapias |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102887732A true CN102887732A (en) | 2013-01-23 |
| CN102887732B CN102887732B (en) | 2013-11-20 |
Family
ID=47531434
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012104239991A Expired - Fee Related CN102887732B (en) | 2012-10-30 | 2012-10-30 | Method for producing amino acid liquid fertilizer by processing wastewater via tilapias |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102887732B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103880482A (en) * | 2014-03-21 | 2014-06-25 | 国农生物肥料连云港有限责任公司 | Preparation method of crop organic fertilizer |
| CN104591808A (en) * | 2014-12-30 | 2015-05-06 | 浙江海洋学院 | Method for fermenting aquatic product leftovers by mixed culture bacteria |
| CN104892195A (en) * | 2015-06-10 | 2015-09-09 | 苏州御亭现代农业产业园发展有限公司 | Organic biological enzyme compound fertilizer for Chinese gooseberry |
| CN105254344A (en) * | 2015-10-29 | 2016-01-20 | 广东省农业科学院蚕业与农产品加工研究所 | Method for preparing liquid amino acid liquid fertilizer from tilapia mossambica processed wastes, liquid amino acid liquid fertilizer and application of liquid amino acid liquid fertilizer |
| CN109180230A (en) * | 2018-10-18 | 2019-01-11 | 舟山丰宇三立海洋生物肥料有限公司 | A method of less salt high bioactivity organic fertilizer is produced using fish meal machining cooking water |
| CN110117209A (en) * | 2018-02-05 | 2019-08-13 | 大地还原生物科技股份有限公司 | The manufacturing method of the liquid fertilizer of organic matter containing amino acid |
| CN115812070A (en) * | 2021-06-30 | 2023-03-17 | 华川郡 | Preparation method of fermentation liquor of oncorhynchus masou |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1136029A (en) * | 1994-05-17 | 1996-11-20 | 深圳宝安区科技开发公司 | Treatment of waste liquid of monosodium glutamate |
| CN1207380A (en) * | 1997-08-04 | 1999-02-10 | 汤洁 | Method for preparing functional compound liquid fertilizer using marine living things |
| CN1597626A (en) * | 2004-08-27 | 2005-03-23 | 中国海洋大学 | Preparation method of fish protein biological peptide fertilizer |
| KR100825639B1 (en) * | 2007-03-16 | 2008-04-25 | 장창순 | The preparation method of liquid fertilizer containing the amino acid from the high concentration organic wastewater as raw materials |
-
2012
- 2012-10-30 CN CN2012104239991A patent/CN102887732B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1136029A (en) * | 1994-05-17 | 1996-11-20 | 深圳宝安区科技开发公司 | Treatment of waste liquid of monosodium glutamate |
| CN1207380A (en) * | 1997-08-04 | 1999-02-10 | 汤洁 | Method for preparing functional compound liquid fertilizer using marine living things |
| CN1597626A (en) * | 2004-08-27 | 2005-03-23 | 中国海洋大学 | Preparation method of fish protein biological peptide fertilizer |
| KR100825639B1 (en) * | 2007-03-16 | 2008-04-25 | 장창순 | The preparation method of liquid fertilizer containing the amino acid from the high concentration organic wastewater as raw materials |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103880482A (en) * | 2014-03-21 | 2014-06-25 | 国农生物肥料连云港有限责任公司 | Preparation method of crop organic fertilizer |
| CN103880482B (en) * | 2014-03-21 | 2016-04-13 | 国农生物肥料连云港有限责任公司 | A kind of preparation method of crop organic fertilizer |
| CN104591808A (en) * | 2014-12-30 | 2015-05-06 | 浙江海洋学院 | Method for fermenting aquatic product leftovers by mixed culture bacteria |
| CN104892195A (en) * | 2015-06-10 | 2015-09-09 | 苏州御亭现代农业产业园发展有限公司 | Organic biological enzyme compound fertilizer for Chinese gooseberry |
| CN105254344A (en) * | 2015-10-29 | 2016-01-20 | 广东省农业科学院蚕业与农产品加工研究所 | Method for preparing liquid amino acid liquid fertilizer from tilapia mossambica processed wastes, liquid amino acid liquid fertilizer and application of liquid amino acid liquid fertilizer |
| CN110117209A (en) * | 2018-02-05 | 2019-08-13 | 大地还原生物科技股份有限公司 | The manufacturing method of the liquid fertilizer of organic matter containing amino acid |
| CN109180230A (en) * | 2018-10-18 | 2019-01-11 | 舟山丰宇三立海洋生物肥料有限公司 | A method of less salt high bioactivity organic fertilizer is produced using fish meal machining cooking water |
| CN115812070A (en) * | 2021-06-30 | 2023-03-17 | 华川郡 | Preparation method of fermentation liquor of oncorhynchus masou |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102887732B (en) | 2013-11-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102887732B (en) | Method for producing amino acid liquid fertilizer by processing wastewater via tilapias | |
| CN102948614B (en) | Method for preparing peptide used for active feed by bacteria and enzyme synergistic fermentation bean pulp | |
| CN103931878B (en) | Discarded cassava grain stillage liquid is utilized to prepare the method for high protein feed | |
| CN105454658A (en) | Preparation method of feather fermentation protein feed | |
| CN106244658B (en) | Preparation method of sweet potato protein polypeptide | |
| CN102994605B (en) | Method for producing high-efficiency biological peptide through enzymolysis and microbe fermentation | |
| CN102475266A (en) | Preparation method of natural zinc-rich oyster powder | |
| CN110256603A (en) | A kind of-two step enzyme method coupling of shrimp and crab shells hydro-thermal prepares the methods and applications of chitin and chitosan | |
| CN105249464A (en) | Preparation method of shrimp peptides chelated calcium | |
| CN105695546A (en) | Method for preparing liquid organic fertilizer through fermentation of aquatic product waste | |
| CN101869169A (en) | Method for preparing fish oligopeptide from gurry by combining fermentation and membrane technology | |
| CN104351464A (en) | Preparation method of soybean peptide powder | |
| CN103555804A (en) | Preparation method of animal and plant mixed protein peptide | |
| CN105385748A (en) | Preparation method for fermentative production of marine peptides streptozotocin by using sea cucumber leftovers | |
| CN113122603A (en) | Process for preparing enzymolysis fish soluble paste and functional protein peptide | |
| CN103421871A (en) | Preparation method of tuna bone collagen peptide | |
| CN105483195A (en) | Method for preparing marine protein peptide by multi-step enzymolysis | |
| CN102517367B (en) | Method for preparing collagen anti-oxidation peptide by using pig skin | |
| CN101720905B (en) | Flavor yeast albumen powder and preparation method thereof | |
| CN103518943A (en) | Method for extracting proteins from rape seed cakes by utilizing multi-frequency ultrasonic waves | |
| CN101912036B (en) | Method for preparing whole blood hydrolysate of livestock and poultry | |
| CN108925747A (en) | A kind of functional protein and its method using aquatic product protein and saccharomycete preparation | |
| CN104341181A (en) | Multifunctional fermented kelp liquid and manufacturing method thereof | |
| CN106072070A (en) | The production method of the health instant powder that a kind of fish skin is compounding with probiotic bacteria | |
| CN108782948B (en) | Yeast-derived functional protein and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131120 Termination date: 20161030 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |