CN101869169A - Method for preparing fish oligopeptide from gurry by combining fermentation and membrane technology - Google Patents
Method for preparing fish oligopeptide from gurry by combining fermentation and membrane technology Download PDFInfo
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Abstract
The invention relates to a method for preparing fish oligopeptide from gurry by combining fermentation and membrane technology, which comprises the following steps of: (1) crushing the gurry, adding water to make the water account for 40 to 70 weight percent of the gurry, sterilizing for 10 to 30 minutes at the temperature of 115 DEG C, adding mixed strains in an amount which accounts for 2 to 8 percent of dry weight of the gurry to perform fermentation after the temperature is reduced to between 25 and 45 DEG C, adjusting the pH value to between 6.5 and 8.0 and performing fermentation for 36 to 96 hours; (2) after extracting collagen oligopeptide or mixed protein oligopeptide from fermentation liquor by washing, performing decolorization and rough filtration, and then purifying, disinfecting, refining, concentrating and desalting hydrolysate by using a microfiltration film, an ultrafiltration film and a nanofiltration film; and (3) further concentrating liquor concentrated by the third-level film by circulating at a low temperature, and then performing vacuum freeze drying to obtain fish collagen oligopeptide or fish mixed protein oligopeptide. The method effectively solves the problems of high cost, high energy consumption, and low purity and activity of products existing in the conventional fish oligopeptide production process.
Description
Technical field
The present invention relates to a kind of method of producing fish oligopeptide, particularly relate to the mixed culture fermentation of a kind of employing mixed bacteria, with fish offal material such as fish head, fish scale, fish-skin, fish tail, shark's fin adherent meats is raw material, the binding film separation process technique, the method for on a production line, producing Isin glue collagen oligopeptide or fish mixed protein oligopeptide.
Background technology
China is world fish culture big country, accounts for about 1/3 of Gross World Product, occupies the first in the world.Though possess advantageous aquatic products fish resource advantage, the utilization of fish resource still rests on the comparatively original stage, substantially with the need of fresh and alive form sale for food and drink.Though small part is arranged through being processed into products such as fish gruel, can and Cheng Ganyu, all belongs to elementary processing mode.In fish preliminary working process, produce a large amount of preliminary working leftover bits and pieces, account for 30%～40% of fish gross weight as fish head, fish tail, fish scale, fish-skin, fish guts etc., these leftover bits and pieces or directly abandon, simply being processed as feed sells at a low price, not only value is not fully utilized, even also pollutes the environment.And the flesh of fish that fish head, fish tail, fish intestines and shark's fin adhere to is rich in multiple proteins, fish scale and fish-skin contain abundant Isin glue collagen, if fully development and use can be turned waste into wealth, prepare fish mixed protein oligopeptide and collagen oligopeptide respectively with high added value.
Oligopeptide is a kind of important biological material, be easily absorbed by the body and have higher physiological activities, mainly show: regain one's strength of body, regulate aspects such as immunity, anti-hypertension, anti-oxidant, antifatigue fast, can be widely used in industry fields such as food, health products, cosmetics, and has consumption market widely, abroad and form huge consumption market, domestic demand is also enlarging gradually.
Chinese patent: CN 200810195646.4 " endogenous enzymes prepares the technical study of bioactive micro peptide " discloses a kind of technology of utilizing endogenous enzymes hydrolysis marine products low value fish offal to prepare bioactive micro peptide, this technology comprises the endogenous enzymes preliminary treatment, enzymolysis, screen filtration, concentrate, drying and other steps, it is low that this scheme exists endogenous enzymes hydrolysis power, the endogenous enzymes of fish body itself is not enough to the fish offal of hydrolysis correspondence, lack and separate, removal of impurities and subtractive process, the long peptide chain and the little molecule free amino acid of abundant hydrolysis that exist in the enzymolysis liquid are not effectively separated and removal of impurities, the purity of biologically active peptide is not high, concentrates and problem such as dry run energy consumption height.
Summary of the invention
The present invention is in order to overcome above-mentioned problems of the prior art and shortcoming, a kind of method for preparing fish oligopeptide with fish offal material fermentation coupled film technology is provided, fish oligopeptide comprises Isin glue collagen oligopeptide and fish mixed protein oligopeptide, this method is with the fish offal material after pulverizing, adopt multiple bacteria compound fermentation, the isolation technics of using by three grades of symphysis is carried out removal of impurities to hydrolyzate again, degerming, refining, concentrate and desalination, collecting concentrate concentrates further concentrated through circulation low temperature, after vacuum freeze drying, get the oligopeptide product again, efficiently solve cost height in the original fish oligopeptide production technology, the energy consumption height, low and the active low problem of product of product purity.
The technical solution adopted in the present invention is:
The method for preparing fish oligopeptide with fish offal material fermentation coupled film technology, it is characterized in that carrying out according to the following steps: (1) pulverizes the fish offal material, the percentage by weight that adds water in the entry adjustment fish offal material is 40%～70%, in 115 ℃ of sterilizations 10-30 minute, equitemperature drops to 25～45 ℃, the fermented by mixed bacterium that adds the dry weight 2%～8% that accounts for the fish offal material is regulated the pH value to 6.5-8.0, ferments 36～96 hours; (2) zymotic fluid is after water elution goes out collagen oligopeptide or mixed protein oligopeptide, after decolouring, coarse filtration, carry out micro-filtration, ultrafiltration, three grades of symphysis of nanofiltration with the removal of impurities of the liquid that is hydrolyzed, degerming, refining, concentrate and desalination; (3) through the concentrate of third level film again after circulation low temperature further concentrates, carry out vacuum freeze drying again and get Isin glue collagen oligopeptide or fish mixed protein oligopeptide.
In such scheme, described fish offal material is for mixing leftover bits and pieces, single fish scale leftover bits and pieces or single fish-skin leftover bits and pieces, with to mix leftover bits and pieces be raw material through fermenting and producing be fish mixed protein oligopeptide, with single fish scale or single fish-skin be raw material production be the collagen oligopeptide.
In such scheme, the percentage by weight of water is 40%～60% in the preceding fish offal material of described fermentation.
In such scheme, the temperature of described fermentation is 28～35 ℃.
In such scheme, described many bacterial classifications refer to two kinds in aspergillus terricola, actinomyces, the bacillus pumilus.
In such scheme, 2%～6% of the dry weight that described fermented bacterium inoculum concentration is the fish offal material.
In such scheme, described fermentation time is 64～80 hours.
The advantage of technical solution of the present invention is:
1, a production line is produced 2 kinds of different products, satisfies the different market demands, has saved the industrial production input cost.
2, adopt multiple bacteria compound fermentation, solve the present situation problem of the low and commercially available protease price costliness of traditional endogenous enzymes hydrolysis efficiency; Fermentation replaces traditional mode of production Isin glue collagen peptide acid-base pretreatment process, avoids widely applying the flushing of chemical reagent such as acid, alkali and great lot of water resources, has saved resource, has protected environment.
3, the effective multiple proteins component in the aminosal functional areas of this project technology discharges multiple active peptide with biological function, has improved the product activity content of material to greatest extent.
4, the removal of impurities after adopting membrane technology to be used to ferment, refining and concentrate can obtain high-purity, molecular weight concentrates on the product about 500Da.Simultaneously the volume of polypeptide solution can be reduced to 1/4th of zymotic fluid, greatly reduce the required energy consumption of follow-up drying process, can also accomplish that in addition process water recycles, both reduce water resources consumption, reduce production waste again.
5, adopt one step of film desalination and concentration to finish, cut resin desalination operation, peptide loses problem big and the generation large amount of sewage when having avoided the resin desalination.
6, adopt the low temperature circulation to concentrate and freeze drying process, replaces that traditional economic benefits and social benefits are concentrated, drying process with atomizing.Whole technical process is in normal temperature or cryogenic conditions, the oligopeptide loss of activity of avoiding high temperature to cause, and product activity is protected.
Compare with other prior aries, the present invention concentrates on about 500Da with the fish mixed protein oligopeptide molecular weight overwhelming majority of fish mixing waste production, is easy to absorption of human body, and the biologically active high product does not have bitter taste, and mouthfeel is joyful; Technology is that the Isin glue collagen oligopeptide molecular weight of raw material production is concentrated more than 70% and is distributed in about 500Da with fish-skin, fish phosphorus, product yield (dry fish scale) is more than 34%, collagen peptide purity has good antioxidant effect and activating cell function effect more than 90%.
Multiple bacteria compound fermentation has not only solved the low present situation of endogenous enzymes percent hydrolysis, has more avoided adding the high cost of commercially available protease, also has the effect of directionally hydrolyzing, can hydrolysis go out to have higher bioactive oligopeptide product; The film processing procedure can realize the removal of impurities of hydrolyzate, refining, concentrated and desalination, improves the purity of product, and the low temperature circulation concentrates and can further concentrate the NF membrane concentrate, makes dry treating capacity minimizing 3/4, has greatly reduced drying cost; Can realize that by the fine setting zymotechnique two kinds of products of a production line production to satisfy the different market demands, have widened market and reduced the investment in fixed assets cost.
The specific embodiment
Embodiment 1
Get fish and mix leftover bits and pieces 100Kg pulverizing, the percentage by weight that adds water in the entry adjustment fish offal material is 60%, in 115 ℃ of high-temperature sterilizations 20 minutes, equitemperature drops to 30 ℃, insert the bacillus pumilus and the native bacillus that dwells, the bacillus pumilus and the native bacillus ratio mixing in 1: 1 by weight of dwelling, the bacterial classification amount is 2% of a fish mixing leftover bits and pieces dry weight, and adjusting pH value to 8.0, fermented with this understanding 64 hours, and then be warming up to 45 ℃, constant temperature 8 hours, wash zymotic fluid then 2 times, extract oligopeptide, merge water lotion twice, 30min again decolours, filter cloth filters, and protein extracting ratio is 93%; Hydrolyzate after the coarse filtration is selected the removal of impurities of 10nm micro-filtration membrane, degerming for use, and operating condition is: operating pressure P=0.2MPa, work temperature=50 ℃, flow velocity Q=60L/min; Select for use molecular cut off 2500Da milipore filter refining, remove the peptide of most of molecular weight greater than 1000Da, see through the oligopeptide that molecular weight is lower than 1000Da, operating condition is: P=0.5MPa, T=40 ℃, Q=30L/min; Select for use the NF membrane that sees through molecular weight 150～200Da to carry out desalination, concentrate, operating condition is: P=1.5MPa, T=40 ℃, Q=20L/min.Concentrate the final vacuum freeze drying through the low temperature circulation again and can get 9.9kg fish mixed protein oligopeptide powder.
Embodiment 2
Fish mixes leftover bits and pieces 100kg and pulverizes, the percentage by weight that adds water in the entry adjustment fish offal material is 70%, in 115 ℃ of high-temperature sterilizations 10 minutes, equitemperature drops to 35 ℃, insert bacillus pumilus and actinomyces, bacillus pumilus and actinomyces 1: 1 ratio by weight mix, inoculum concentration is 2% of a fish mixing leftover bits and pieces dry weight, and regulate pH value to 7.5, and fermented with this understanding 28 hours, then be warming up to 45 ℃ of constant temperature 8 hours, zymotic fluid water is carried 2 times, water liquid uses active carbon in 50 ℃ of decolourings 30 minutes again, and the coarse filtration rear filtrate is handled through film, and protein extracting ratio is 94%; Film processing procedure and operating condition obtain 10.1kg fish mixed protein oligopeptide powder at last with case study on implementation 1.
Embodiment 3
Dry fish scale 80kg pulverizes, the percentage by weight that adds water in the entry adjustment fish offal material is 40%, in 115 ℃ of high-temperature sterilizations 30 minutes, equitemperature drops to 25 ℃, insert aspergillus terricola and actinomyces, aspergillus terricola and actinomyces 1: 1 ratio by weight mix, inoculum concentration is 6% of a fish scale dry weight, and regulate pH value to 6.5, and fermented with this understanding 72 hours, then be warming up to 45 ℃ of constant temperature 8 hours, zymotic fluid water is carried 2 times, water liquid uses active carbon in 50 ℃ of decolourings 30 minutes again, and the coarse filtration rear filtrate is handled through film, and protein extracting ratio is 92%; Film processing procedure and operating condition obtain 27.2kg Isin glue collagen oligopeptide powder at last with case study on implementation 1.
Embodiment 4
Dry fish scale 80kg pulverizes, add entry and adjust the percentage by weight 50% of water in the fish offal material, in 115 ℃ of high-temperature sterilizations 20 minutes, equitemperature drops to 28 ℃, insert aspergillus terricola and bacillus pumilus, aspergillus terricola and bacillus pumilus 1: 1 ratio by weight mix, inoculum concentration is 8% of a fish scale dry weight, and regulate pH value to 7, and fermented with this understanding 56 hours, then be warming up to 45 ℃ of constant temperature 8 hours, zymotic fluid water is carried 2 times, water liquid uses active carbon in 50 ℃ of decolourings 30 minutes again, and the coarse filtration rear filtrate is handled through film, and protein extracting ratio is 90%; Film processing procedure and operating condition obtain 26.9kg Isin glue collagen oligopeptide powder at last with case study on implementation 1.
Embodiment 5
Dried fish-skin 80kg pulverizes, add entry and adjust the percentage by weight 50% of water in the fish offal material, in 115 ℃ of high-temperature sterilizations 20 minutes, equitemperature drops to 35 ℃, insert aspergillus terricola and bacillus pumilus, aspergillus terricola and bacillus pumilus 1: 1 ratio by weight mix, inoculum concentration is 5% of a fish scale dry weight, and regulate pH value to 7.5, and fermented with this understanding 88 hours, then be warming up to 45 ℃ of constant temperature 8 hours, zymotic fluid water is carried 2 times, water liquid uses active carbon in 50 ℃ of decolourings 30 minutes again, and the coarse filtration rear filtrate is handled through film, and protein extracting ratio is 91%; Film processing procedure and operating condition obtain 26.9kg Isin glue collagen oligopeptide powder at last with case study on implementation 1.
Claims (7)
1. the method for preparing fish oligopeptide with fish offal material fermentation coupled film technology, it is characterized in that carrying out according to the following steps: (1) pulverizes the fish offal material, the percentage by weight that adds water in the entry adjustment fish offal material is 40%～70%, in 115 ℃ of sterilizations 10-30 minute, equitemperature drops to 25～45 ℃, the fermented by mixed bacterium that adds the dry weight 2%～8% that accounts for the fish offal material is regulated the pH value to 6.5-8.0, ferments 36～96 hours; (2) zymotic fluid is after water elution goes out collagen oligopeptide or mixed protein oligopeptide, after decolouring, coarse filtration, carry out micro-filtration, ultrafiltration, three grades of symphysis of nanofiltration with the removal of impurities of the liquid that is hydrolyzed, degerming, refining, concentrate and desalination; (3) through the concentrate of third level film again after circulation low temperature further concentrates, carry out vacuum freeze drying again and get Isin glue collagen oligopeptide or fish mixed protein oligopeptide.
2. method according to claim 1 is characterized in that: described fish offal material is for mixing leftover bits and pieces, single fish scale leftover bits and pieces or single fish-skin leftover bits and pieces.
3. method according to claim 1 is characterized in that: the percentage by weight of water is 40%～60% in the preceding fish offal material of described fermentation.
4. method according to claim 1 is characterized in that: the temperature of described fermentation is 28～35 ℃.
5. method according to claim 1 is characterized in that: described mixed bacteria refers to two kinds in aspergillus terricola, actinomyces, the bacillus pumilus.
6. method according to claim 1 is characterized in that: 2%～6% of the dry weight that described fermented bacterium inoculum concentration is the fish offal material.
7. method according to claim 1 is characterized in that: described fermentation time is 64～80 hours.
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Cited By (8)
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| CN102199646A (en) * | 2011-03-15 | 2011-09-28 | 广东海洋大学 | Method for preparing collagen peptide with fish skin |
| US20120107409A1 (en) * | 2010-11-03 | 2012-05-03 | Food Industry Research And Development Institute | Preparation and use of fish skin fermentation product |
| CN102492761A (en) * | 2011-12-02 | 2012-06-13 | 安徽燕之坊食品有限公司 | Method for preparing active polypeptide from fish pomace |
| CN102517367A (en) * | 2011-12-15 | 2012-06-27 | 河南科技大学 | Method for preparing collagen anti-oxidation peptide by using pig skin |
| JP2012532629A (en) * | 2010-09-28 | 2012-12-20 | 中国科学院南海海洋研究所 | Marine-derived Bacillus barbaricus SCSIO02429 and method for preparing squid oligopeptide using the same |
| CN103211199A (en) * | 2013-05-13 | 2013-07-24 | 武汉梁子湖水产品加工有限公司 | Method for preparing fresh fish essence through processed freshwater fish leftover binary fermentation |
| CN105285312A (en) * | 2014-06-12 | 2016-02-03 | 上海中科国嘉技术转移有限公司 | Preparation method of seafood flavor peptide |
| CN109953176A (en) * | 2017-12-23 | 2019-07-02 | 青岛麦迪尔生物工程有限公司 | A method of peptone is prepared using aquatic products processing leftover bits and pieces |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2012532629A (en) * | 2010-09-28 | 2012-12-20 | 中国科学院南海海洋研究所 | Marine-derived Bacillus barbaricus SCSIO02429 and method for preparing squid oligopeptide using the same |
| US20120107409A1 (en) * | 2010-11-03 | 2012-05-03 | Food Industry Research And Development Institute | Preparation and use of fish skin fermentation product |
| CN102199646A (en) * | 2011-03-15 | 2011-09-28 | 广东海洋大学 | Method for preparing collagen peptide with fish skin |
| CN102199646B (en) * | 2011-03-15 | 2014-08-13 | 广东海洋大学 | Method for preparing collagen peptide with fish skin |
| CN102492761A (en) * | 2011-12-02 | 2012-06-13 | 安徽燕之坊食品有限公司 | Method for preparing active polypeptide from fish pomace |
| CN102517367A (en) * | 2011-12-15 | 2012-06-27 | 河南科技大学 | Method for preparing collagen anti-oxidation peptide by using pig skin |
| CN102517367B (en) * | 2011-12-15 | 2014-04-02 | 河南科技大学 | Method for preparing collagen anti-oxidation peptide by using pig skin |
| CN103211199A (en) * | 2013-05-13 | 2013-07-24 | 武汉梁子湖水产品加工有限公司 | Method for preparing fresh fish essence through processed freshwater fish leftover binary fermentation |
| CN103211199B (en) * | 2013-05-13 | 2014-09-24 | 武汉梁子湖水产品加工有限公司 | Method for preparing fresh fish essence through processed freshwater fish leftover binary fermentation |
| CN105285312A (en) * | 2014-06-12 | 2016-02-03 | 上海中科国嘉技术转移有限公司 | Preparation method of seafood flavor peptide |
| CN109953176A (en) * | 2017-12-23 | 2019-07-02 | 青岛麦迪尔生物工程有限公司 | A method of peptone is prepared using aquatic products processing leftover bits and pieces |
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