CN102115690A - Method for comprehensively utilizing rice bran - Google Patents
Method for comprehensively utilizing rice bran Download PDFInfo
- Publication number
- CN102115690A CN102115690A CN2011100048401A CN201110004840A CN102115690A CN 102115690 A CN102115690 A CN 102115690A CN 2011100048401 A CN2011100048401 A CN 2011100048401A CN 201110004840 A CN201110004840 A CN 201110004840A CN 102115690 A CN102115690 A CN 102115690A
- Authority
- CN
- China
- Prior art keywords
- rice bran
- peptide
- solution
- rice
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention discloses a method for comprehensively utilizing rice bran, which can be used for preparing four types of products, namely rice bran oil, rice bran crude fiber, rice bran egg white and rice bran peptide. A preparation process comprises the following steps of: extracting grease from rice bran serving as a raw material; separating rice bran crude fiber and rice bran egg white from the rice bran by using an alkali-solution and acid-isolation method; adding protease into the rice bran egg white for hydrolyzing proteins; removing salt through anion and cation exchange resins by ultrafiltration fraction; and concentrating and drying to obtain rice bran biologically active peptide.
Description
Technical field
The present invention relates to a kind of method that fully utilizes rice bran.
Background technology
Paddy is the first grain variety of China.Produce about 1.85 hundred million tons per year at present, account for 42% of national total output of grain.Rice yield accounts for 37% of total output of grain in the world.Paddy will remove shell and account for the kind skin and the embryo of gross weight about 10% in being processed into the process of polished rice, rice bran is processed into by kind of skin and embryo, is the main byproduct of paddy processing.Domestic and international research result and data show, are rich in various nutrient substances and physiologically active substance in the rice bran.Because the raw material of processing rice bran is different with the processing technology that is adopted, the moiety of rice bran is not just the same.In general, on average contain protein 15% in the rice bran, fat 16%～22%, sugar 3%～8%, moisture 10% etc.
At present, more single to the processing mode of rice bran both at home and abroad, do not have a kind of comprehensive utilization method, or only extract Rice pollard oil, or only extract the rice bran food fibre, and less to utilizing of rice bran protein and rice bran peptide.The preparation technology of existing rice bran peptide mainly contains three kinds: (1) gene engineering method; (2) chemical synthesis process; (3) enzymolysis process.Preparing in the technology of rice bran peptide with enzymolysis process at present, in enzymolysis process, all add the potential of hydrogen of a large amount of alkali lye or acid solution solution when regulating enzyme digestion reaction, and do not have desalinating process, make finished product salinity too high levels; And, the finished product are not carried out classification, the molecular weight distribution difference of the finished product is bigger, has influenced the physiologically active of product.
Summary of the invention
Problem to be solved by this invention provides a kind of rice bran comprehensive utilization method, can prepare Rice pollard oil, rice-bran crude fiber, rice bran protein, 4 kinds of products of rice bran peptide.
In order to solve the problems of the technologies described above, rice bran method of comprehensive utilization provided by the invention is: be raw material with the rice bran, extract the grease in the rice bran earlier, use " alkali extraction and acid precipitation " method to separate rice-bran crude fiber and rice bran protein again, then rice bran protein is added proteolytic enzyme protolysate matter, pass through the ultrafiltration classification again, cross anion-cation exchange resin except that freshen, concentrated at last, drying obtains the rice bran biologically active peptides.
A kind of method that fully utilizes rice bran of the present invention can be following steps:
(1) rice bran is pulverized, crossed 60～80 mesh sieves, adopt the Soxhlet extraction process,, then hexane solution is filtered, rice bran airing to normal hexane is volatilized fully, be defatted rice bran with 95% normal hexane solvent lixiviate 2h under 50 ℃ of conditions; Filtrate is evaporated normal hexane fully with rotatory evaporator, and remaining is Rice pollard oil;
(2) step (1) defatted rice bran and water are dissolved in the water by 1: 5～1: 10 (mass ratio), be heated to 45～55 ℃, regulate pH to 8.0～10.0 with NaOH reagent, do not stop to stir 15min, under the rotating speed of 3000～4000r/min, centrifugal 15min gets supernatant liquor, to precipitate oven dry, be the rice-bran crude fiber;
(3) supernatant liquor with step (2) is heated to 70～90 ℃, regulates pH to 3.0～5.0 with citric acid reagent, does not stop to stir 10min, 3000～4000r/min, and 15min, centrifugal collecting precipitation is rice bran protein, oven dry can get the rice bran protein powder;
(4) rice bran protein and the water with step (3) is dissolved in the water by 1: 5～1: 10 (mass ratio), be heated to 90～100 ℃, keep 15～30min, be cooled to 35～50 ℃ then, regulate and keep pH with NaOH reagent, press E/S than 3%～7% adding neutral protease As1.398, enzymolysis 2～4h 6.0～8.0, constantly stir in the enzymolysis process, and keep the pH value 6.0～8.0 with NaOH solution;
(5) after step to be treated (4) enzymolysis finishes, regulate pH to 5.0～3.0 with HCl solution rapidly, under the rotating speed of 3000～4000r/min, centrifugal 15min gets supernatant liquor, and gained is the rough liquid of rice bran peptide;
(6) the rough liquid of the rice bran peptide of step (5) gained is injected storage tank, solution is the 1000Da ultra-filtration membrane by the molecular weight interception behind micro-filtration, small-molecule substance in the solution infiltrates through the inwall of hollow-fibre membrane becomes ultrafiltrated, collects ultrafiltrated, is rice bran peptide ultrafiltrated; Macromolecular substance such as the albumen in the solution are kept and are concentrated, and concentrated solution is recycled in the step 4 enzymolysis once more;
(7) exchange resin immersion treatment in deionized water was adorned post after 24 hours, with 7.5% hydrochloric acid and 10%NaOH it was soaked respectively then, was converted into H
+Type Zeo-karb and OH
-Type anionite-exchange resin is 7.0 stand-by with washed with de-ionized water to pH again;
With the ultrafiltrated of gained in the step (6), pass through H with the flow velocity of 5～10 times of column volume/h
+The type Zeo-karb removes positively charged ion, stops application of sample when treating the effluent liquid pH=4.0 left and right sides, afterwards this effluent liquid is passed through OH with same flow velocity
-Type anionite-exchange resin removes negatively charged ion, is pH to effluent liquid and stops application of sample at 7.0 o'clock, is collected as rice bran peptide refined liquid;
(8) by the rice bran peptide refined liquid that obtains in the step 6, be evaporated to solid content 20%～30%;
(9) after purified rice bran peptide liquid concentrated, dry powder was made in spray-dried or lyophilize.
The purpose of above-mentioned steps (1) is: fat content is about 16% in the rice bran, utilizes grease to be dissolved in the characteristic of normal hexane, the oil extraction in the rice bran is gone out, and rotary evaporation evaporates normal hexane fully, obtains Rice pollard oil.
The purpose of above-mentioned steps (2) is: utilize protein dissolved characteristic under alkaline condition, rice bran protein and alkali insoluble substance are separated, to improve the content of rice bran protein; The insoluble composition of alkali is the rice-bran crude fiber.
The heavy purpose of above-mentioned steps (3) acid is: utilize protein sedimentary characteristic under acid iso-electric point condition, rice bran protein and acid soluble material are separated, to improve the content of rice bran protein.
The purpose of above-mentioned steps (2), (3) is in order to separate rice-bran crude fiber and rice bran protein in the defatted rice bran.Simultaneously, purifying is used for the rice bran protein of hydrolysis, and proteic content is about 20% in the general defatted rice bran, and after handling through aforesaid method, protein content can reach more than 80%.
The purpose of above-mentioned steps (4) enzymolysis is: with the macro-molecular protein enzymolysis in the rice bran is small protein and peptide;
The purpose of above-mentioned steps (5) acid adjustment is in order to make enzyme deactivation, to stop enzyme digestion reaction; The centrifugal purpose is for solid-liquid separation, collects enzymolysis solution.
The purpose of ultrafiltration is the product that needs molecular weight in order to intercept in the above-mentioned steps (6); The purpose of secondary enzymolysis is for the high molecular weight protein more than the molecular weight 1000Da being hydrolyzed into small molecules, improving and utilize prepared using.
The purpose of desalination is in order to remove in the enzymolysis solution because of constantly regulating the salt ion that pH brings in the enzymolysis process in the above-mentioned steps (7).The product salt content that does not pass through desalting treatment is greater than 8%, and the research and development of products content of process desalting treatment is less than 2%;
The present invention compared with prior art has following advantage and effect:
(1) present method provides a kind of method that fully utilizes rice bran, can prepare Rice pollard oil, rice-bran crude fiber, rice bran protein, 4 kinds of products of rice bran peptide simultaneously;
(2) with the lubricant component in the extraction extraction rice bran, every 100g rice bran is prepared into the about 10g of Rice pollard oil.
(3) utilize the alkali extraction and acid precipitation method to separate rice-bran crude fiber and rice bran protein, crude protein content about 70% in the rice bran protein of extraction, and dietary fiber content about 40% in the rice-bran crude fiber;
(4) adopt ultrafiltration process that the rice bran peptide is carried out stage treatment, guarantee 100% rice bran peptide molecular weight below 1000Da, and carry out secondary enzymolysis for the rice bran peptide more than the molecular weight 1000Da, the molecular weight distribution that makes the finished product is all below 1000Da;
(5) utilize the negative and positive exchange resin that rice bran peptide crude product is carried out desalination, the product salt content that does not pass through desalting treatment is greater than 8%, and the product salt content of process present method desalting treatment is less than 2%;
Description of drawings
The present invention will be further described in detail below in conjunction with the drawings and specific embodiments.
Fig. 1 is the process flow sheet of the method for comprehensive utilization rice bran of the present invention.
Embodiment
E/S described in the present invention is than the mass ratio of consumption that is meant enzyme and rice bran protein; The enzymic activity of neutral protease As1.398 described in the present invention is 130000U/g; Ratio among the present invention all refers to mass ratio if no special instructions.
Embodiment 1
A kind of method that fully utilizes rice bran, this method comprises the steps:
(1) takes by weighing rice bran 100g, pulverized 60 mesh sieves, adopt the Soxhlet extraction process,, then hexane solution is filtered, rice bran airing to normal hexane is volatilized fully, be defatted rice bran with 95% normal hexane solvent lixiviate 2h under 50 ℃ of conditions; Filtrate is evaporated normal hexane fully with rotatory evaporator, and remaining is Rice pollard oil, is weighed as 11.3g;
(2) step (1) defatted rice bran is poured in the 600mL water, be heated to 45 ℃, regulate pH to 9.0 with NaOH reagent, do not stop to stir 15min, under the rotating speed of 3000r/min, centrifugal 15min gets supernatant liquor; To precipitate oven dry,, be weighed as 53.2g for the rice-bran crude fiber;
(3) supernatant liquor with step (2) is heated to 80 ℃, regulates pH to 3.5 with citric acid reagent, does not stop to stir 10min, 3000r/min, and 15min, centrifugal collecting precipitation is rice bran protein, oven dry can get the rice bran protein powder, is weighed as 26.5g;
(4) precipitation with step (3) adds in the 200mL water, be heated to 90 ℃, keep 20min, be cooled to 45 ℃ then, regulate and keep pH with NaOH reagent, add neutral protease As1.398 (enzymic activity is 130000U/g) 1.5g, enzymolysis 2h 7.0, constantly stir in the enzymolysis process, and keep the pH value 7.0 with NaOH solution;
(5) after step to be treated (4) enzymolysis finishes, regulate pH to 4.0 with HCl solution rapidly, under the rotating speed of 3000r/min, centrifugal 15min gets supernatant liquor, and gained is the rough liquid of rice bran peptide;
(6) the rough liquid of the rice bran peptide of step (5) gained is injected storage tank, solution is the 1000Da ultra-filtration membrane by the molecular weight interception behind micro-filtration, small-molecule substance in the solution infiltrates through the inwall of hollow-fibre membrane becomes ultrafiltrated, collects ultrafiltrated, is rice bran peptide ultrafiltrated; Macromolecular substance such as the albumen in the solution are kept and are concentrated, and concentrated solution is recycled in the step 4 enzymolysis once more;
(7) exchange resin immersion treatment in deionized water was adorned post after 24 hours, with 7.5% hydrochloric acid and 10%NaOH it was soaked respectively then, was converted into H
+Type Zeo-karb and OH
-Type anionite-exchange resin is 7.0 stand-by with washed with de-ionized water to pH again;
With the ultrafiltrated of gained in the step (6), pass through H with the flow velocity of 10 times of column volume/h
+The type Zeo-karb removes positively charged ion, stops application of sample when treating the effluent liquid pH=4.0 left and right sides, afterwards this effluent liquid is passed through OH with same flow velocity
-Type anionite-exchange resin removes negatively charged ion, is pH to effluent liquid and stops application of sample at 7.0 o'clock, is collected as rice bran peptide refined liquid;
(8) by the rice bran peptide refined liquid that obtains in the step 6, be evaporated to solid content 30%;
(9) after purified rice bran peptide liquid concentrated, dry powder was made in spray-dried or lyophilize, is weighed as 15.3g.
The rice bran peptide that aforesaid method obtains, rice bran peptide total nitrogen content (in butt) 85.2% after testing, peptide content (in butt) 〉=80.4%, the relative molecular mass of gained is all below 1000Da, moisture content 5.7%, ash oontent (in butt)≤1.6%, crude fat content (in butt)≤0.8%.
Embodiment 2
A kind of method that fully utilizes rice bran, this method comprises the steps:
(1) takes by weighing rice bran 500g, pulverized 60 mesh sieves, adopt the Soxhlet extraction process,, then hexane solution is filtered, rice bran airing to normal hexane is volatilized fully, be defatted rice bran with 95% normal hexane solvent lixiviate 2h under 50 ℃ of conditions; Filtrate is evaporated normal hexane fully with rotatory evaporator, and remaining is Rice pollard oil, is weighed as 58.9g;
(2) step (1) defatted rice bran is poured in the 2.5L water, be heated to 50 ℃, regulate pH to 8.5 with NaOH reagent, do not stop to stir 15min, under the rotating speed of 3000r/min, centrifugal 15min gets supernatant liquor; To precipitate oven dry,, be weighed as 247.3g for the rice-bran crude fiber;
(3) supernatant liquor with step (2) is heated to 90 ℃, regulates pH to 3.0 with citric acid reagent, does not stop to stir 10min, 3000r/min, and 15min, centrifugal collecting precipitation is rice bran protein, oven dry can get the rice bran protein powder, is weighed as 105.6g;
(4) precipitation with step (3) adds in the 1L water, be heated to 90 ℃, keep 20min, be cooled to 40 ℃ then, regulate and keep pH with NaOH reagent, add neutral protease As1.398 (enzymic activity is 130000U/g) 5g, enzymolysis 3h 7.5, constantly stir in the enzymolysis process, and keep the pH value 7.5 with NaOH solution;
(5) after step to be treated (4) enzymolysis finishes, regulate pH to 4.0 with HCl solution rapidly, under the rotating speed of 3000r/min, centrifugal 15min gets supernatant liquor, and gained is the rough liquid of rice bran peptide;
(6) the rough liquid of the rice bran peptide of step (5) gained is injected storage tank, solution is the 1000Da ultra-filtration membrane by the molecular weight interception behind micro-filtration, small-molecule substance in the solution infiltrates through the inwall of hollow-fibre membrane becomes ultrafiltrated, collects ultrafiltrated, is rice bran peptide ultrafiltrated; Macromolecular substance such as the albumen in the solution are kept and are concentrated, and concentrated solution is recycled in the step 4 enzymolysis once more;
(7) exchange resin immersion treatment in deionized water was adorned post after 24 hours, with 7.5% hydrochloric acid and 10%NaOH it was soaked respectively then, was converted into H
+Type Zeo-karb and OH
-Type anionite-exchange resin is 7.0 stand-by with washed with de-ionized water to pH again;
With the ultrafiltrated of gained in the step (6), pass through H with the flow velocity of 10 times of column volume/h
+The type Zeo-karb removes positively charged ion, stops application of sample when treating the effluent liquid pH=4.0 left and right sides, afterwards this effluent liquid is passed through OH with same flow velocity
-Type anionite-exchange resin removes negatively charged ion, is pH to effluent liquid and stops application of sample at 7.0 o'clock, is collected as rice bran peptide refined liquid;
(8) by the rice bran peptide refined liquid that obtains in the step 6, be evaporated to solid content 30%;
(9) after purified rice bran peptide liquid concentrated, dry powder was made in spray-dried or lyophilize, is weighed as 67.6g.
The rice bran peptide that aforesaid method obtains, rice bran peptide total nitrogen content (in butt) 84.1% after testing, peptide content (in butt) 〉=79.5%, the relative molecular mass of gained is all below 1000Da, moisture content 8.1%, ash oontent (in butt)≤1.9%, crude fat content (in butt)≤0.9%.
Embodiment 3
A kind of method that fully utilizes rice bran, this method comprises the steps:
(1) takes by weighing rice bran 1000g, pulverized 80 mesh sieves, adopt the Soxhlet extraction process,, then hexane solution is filtered, rice bran airing to normal hexane is volatilized fully, be defatted rice bran with 95% normal hexane solvent lixiviate 2h under 50 ℃ of conditions; Filtrate is evaporated normal hexane fully with rotatory evaporator, and remaining is Rice pollard oil, is weighed as 110.2g;
(2) step (1) defatted rice bran is poured in the 7L water, be heated to 45 ℃, regulate pH to 9.0 with NaOH reagent, do not stop to stir 15min, under the rotating speed of 3000r/min, centrifugal 15min gets supernatant liquor; To precipitate oven dry,, be weighed as 537.6g for the rice-bran crude fiber;
(3) supernatant liquor with step (2) is heated to 90 ℃, regulates pH to 3.0 with citric acid reagent, does not stop to stir 10min, 3000r/min, and 15min, centrifugal collecting precipitation is rice bran protein, oven dry can get the rice bran protein powder, is weighed as 206.8g;
(4) precipitation with step (3) adds in the 1500mL water, be heated to 90 ℃, keep 20min, be cooled to 45 ℃ then, regulate and keep pH with NaOH reagent, add neutral protease As1.398 (enzymic activity is 130000U/g) 10g, enzymolysis 4h 7.0, constantly stir in the enzymolysis process, and keep the pH value 7.5 with NaOH solution;
(5) after step to be treated (4) enzymolysis finishes, regulate pH to 4.0 with HCl solution rapidly, under the rotating speed of 3000r/min, centrifugal 15min gets supernatant liquor, and gained is the rough liquid of rice bran peptide;
(6) the rough liquid of the rice bran peptide of step (5) gained is injected storage tank, solution is the 1000Da ultra-filtration membrane by the molecular weight interception behind micro-filtration, small-molecule substance in the solution infiltrates through the inwall of hollow-fibre membrane becomes ultrafiltrated, collects ultrafiltrated, is rice bran peptide ultrafiltrated; Macromolecular substance such as the albumen in the solution are kept and are concentrated, and concentrated solution is recycled in the step 4 enzymolysis once more;
(7) exchange resin immersion treatment in deionized water was adorned post after 24 hours, with 7.5% hydrochloric acid and 10%NaOH it was soaked respectively then, was converted into H
+Type Zeo-karb and OH
-Type anionite-exchange resin is 7.0 stand-by with washed with de-ionized water to pH again;
With the ultrafiltrated of gained in the step (6), pass through H with the flow velocity of 10 times of column volume/h
+The type Zeo-karb removes positively charged ion, stops application of sample when treating the effluent liquid pH=4.0 left and right sides, afterwards this effluent liquid is passed through OH with same flow velocity
-Type anionite-exchange resin removes negatively charged ion, is pH to effluent liquid and stops application of sample at 7.0 o'clock, is collected as rice bran peptide refined liquid;
(8) by the rice bran peptide refined liquid that obtains in the step 6, be evaporated to solid content 30%;
(9) after purified rice bran peptide liquid concentrated, dry powder was made in spray-dried or lyophilize, is weighed as 158.9g.
The rice bran peptide that aforesaid method obtains, rice bran peptide total nitrogen content (in butt) 81.8% after testing, peptide content (in butt) 〉=76.2%, the relative molecular mass of gained is all below 1000Da, moisture content 8.5%, ash oontent (in butt)≤1.9%, crude fat content (in butt)≤1.0%.
Claims (1)
1. a method that fully utilizes rice bran is characterized in that this method comprises the steps:
(1) rice bran is pulverized, crossed 60～80 mesh sieves, adopt the Soxhlet extraction process,, then hexane solution is filtered, rice bran airing to normal hexane is volatilized fully, be defatted rice bran with 95% normal hexane solvent lixiviate 2h under 50 ℃ of conditions; Filtrate is evaporated normal hexane fully with rotatory evaporator, and remaining is Rice pollard oil;
(2) step (1) defatted rice bran and water are dissolved in the water by 1: 5～1: 10 (mass ratio), be heated to 45～55 ℃, regulate pH to 8.0～10.0 with NaOH reagent, do not stop to stir 15min, under the rotating speed of 3000～4000r/min, centrifugal 15min gets supernatant liquor, to precipitate oven dry, be the rice-bran crude fiber;
(3) supernatant liquor with step (2) is heated to 70～90 ℃, regulates pH to 3.0～5.0 with citric acid reagent, does not stop to stir 10min, 3000～4000r/min, and 15min, centrifugal collecting precipitation is rice bran protein, oven dry can get the rice bran protein powder;
(4) rice bran protein and the water with step (3) is dissolved in the water by 1: 5～1: 10 (mass ratio), be heated to 90～100 ℃, keep 15～30min, be cooled to 35～50 ℃ then, regulate and keep pH with NaOH reagent, press E/S than 3%～7% adding neutral protease As1.398, enzymolysis 2～4h 6.0～8.0, constantly stir in the enzymolysis process, and keep the pH value 6.0～8.0 with NaOH solution;
(5) after step to be treated (4) enzymolysis finishes, regulate pH to 5.0～3.0 with HCl solution rapidly, under the rotating speed of 3000～4000r/min, centrifugal 15min gets supernatant liquor, and gained is the rough liquid of rice bran peptide;
(6) the rough liquid of the rice bran peptide of step (5) gained is injected storage tank, solution is the 1000Da ultra-filtration membrane by the molecular weight interception behind micro-filtration, small-molecule substance in the solution infiltrates through the inwall of hollow-fibre membrane becomes ultrafiltrated, collects ultrafiltrated, is rice bran peptide ultrafiltrated; Macromolecular substance such as the albumen in the solution are kept and are concentrated, and concentrated solution is recycled in the step 4 enzymolysis once more;
(7) exchange resin immersion treatment in deionized water was adorned post after 24 hours, with 7.5% hydrochloric acid and 10%NaOH it was soaked respectively then, was converted into H
+Type Zeo-karb and OH
-Type anionite-exchange resin is 7.0 stand-by with washed with de-ionized water to pH again;
With the ultrafiltrated of gained in the step (6), pass through H with the flow velocity of 5～10 times of column volume/h
+The type Zeo-karb removes positively charged ion, stops application of sample when treating the effluent liquid pH=4.0 left and right sides, afterwards this effluent liquid is passed through OH with same flow velocity
-Type anionite-exchange resin removes negatively charged ion, is pH to effluent liquid and stops application of sample at 7.0 o'clock, is collected as rice bran peptide refined liquid;
(8) by the rice bran peptide refined liquid that obtains in the step 6, be evaporated to solid content 20%～30%;
(9) after purified rice bran peptide liquid concentrated, dry powder was made in spray-dried or lyophilize.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100048401A CN102115690B (en) | 2011-01-12 | 2011-01-12 | Method for comprehensively utilizing rice bran |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100048401A CN102115690B (en) | 2011-01-12 | 2011-01-12 | Method for comprehensively utilizing rice bran |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102115690A true CN102115690A (en) | 2011-07-06 |
| CN102115690B CN102115690B (en) | 2013-06-19 |
Family
ID=44214616
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011100048401A Active CN102115690B (en) | 2011-01-12 | 2011-01-12 | Method for comprehensively utilizing rice bran |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102115690B (en) |
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102266004A (en) * | 2011-08-10 | 2011-12-07 | 江西金农生物科技有限公司 | Edible defatted rice bran and preparation method thereof |
| CN102919510A (en) * | 2012-11-29 | 2013-02-13 | 南京财经大学 | Preparation method of rice bran selenoprotein powder |
| CN103461646A (en) * | 2013-09-29 | 2013-12-25 | 东北农业大学 | Method suitable for removing arsenic from rice bran |
| CN103519196A (en) * | 2013-10-21 | 2014-01-22 | 江苏丹绿食品股份有限公司 | Production technology of rice bran dietary fiber |
| CN103549110A (en) * | 2013-11-04 | 2014-02-05 | 中南林业科技大学 | Method for extracting water-soluble rice bran proteins |
| CN104178336A (en) * | 2014-08-29 | 2014-12-03 | 邹平健源油脂有限公司 | Preparation method of millet bran oil |
| CN104397339A (en) * | 2014-11-28 | 2015-03-11 | 江苏康之源粮油有限公司 | Manufacturing method of degreased rice bran |
| CN104754955A (en) * | 2012-10-22 | 2015-07-01 | 帝斯曼知识产权资产管理有限公司 | Mild hydrolysis of proteins from rice bran |
| CN104830927A (en) * | 2015-04-14 | 2015-08-12 | 无锡群硕谷唐生物科技有限公司 | Method for preparing ferulic acid oligosaccharide syrup by using wheat bran |
| CN105400857A (en) * | 2015-12-12 | 2016-03-16 | 常州大学 | Method for extracting antioxidative peptide from degreased rice bran |
| CN106561969A (en) * | 2016-10-18 | 2017-04-19 | 大连理工大学 | Technical method for extracting proteins from rice bran meal through repeated freezing and thawing of auxiliary weak alkali |
| CN107988297A (en) * | 2017-11-27 | 2018-05-04 | 丸美化妆品株式会社 | The application of a kind of preparation method and vinasse small-molecular peptides of vinasse small-molecular peptides in skin care item |
| CN108208506A (en) * | 2018-01-18 | 2018-06-29 | 浙江省柑桔研究所 | A kind of millet cavings beverage and preparation method thereof |
| CN108850550A (en) * | 2018-06-25 | 2018-11-23 | 天柱县森源米业有限责任公司 | A kind of defatted rice bran multi-strain fermentation product |
| CN109602660A (en) * | 2019-01-17 | 2019-04-12 | 上海灰灵生物科技有限公司 | A kind of cosmetic formulations and preparation method thereof rich in Antioxidative Factors |
| CN111440012A (en) * | 2020-04-16 | 2020-07-24 | 上海农乐生物制品股份有限公司 | Preparation method of low-chlorine amino acid fertilizer |
| CN112244297A (en) * | 2020-10-24 | 2021-01-22 | 山西大学 | Preparation method and application of furfuryl alkali extract |
| CN112481022A (en) * | 2020-12-22 | 2021-03-12 | 霍邱县敏东米业有限公司 | Method for synchronously preparing rice bran oil and high-purity active peptide by using rice bran |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1439625A (en) * | 2003-03-17 | 2003-09-03 | 江南大学 | Preparation for active octacosa alkanol and triaconta alkanol in rice bran |
| CN101301067A (en) * | 2008-06-06 | 2008-11-12 | 浙江大学 | Method for synthetically extracting active matters in rice bran with separation technique |
| CN101575620A (en) * | 2009-06-10 | 2009-11-11 | 北京化工大学 | Method for producing fermentation raw material, rice bran oil and protein feed by comprehensively utilizing rice bran |
-
2011
- 2011-01-12 CN CN2011100048401A patent/CN102115690B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1439625A (en) * | 2003-03-17 | 2003-09-03 | 江南大学 | Preparation for active octacosa alkanol and triaconta alkanol in rice bran |
| CN101301067A (en) * | 2008-06-06 | 2008-11-12 | 浙江大学 | Method for synthetically extracting active matters in rice bran with separation technique |
| CN101575620A (en) * | 2009-06-10 | 2009-11-11 | 北京化工大学 | Method for producing fermentation raw material, rice bran oil and protein feed by comprehensively utilizing rice bran |
Non-Patent Citations (1)
| Title |
|---|
| 《粮食加工》 20081231 何欢等 米糠生物活性肽生产工艺的研究 38-39,41 1 第33卷, 第1期 * |
Cited By (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102266004A (en) * | 2011-08-10 | 2011-12-07 | 江西金农生物科技有限公司 | Edible defatted rice bran and preparation method thereof |
| CN104754955A (en) * | 2012-10-22 | 2015-07-01 | 帝斯曼知识产权资产管理有限公司 | Mild hydrolysis of proteins from rice bran |
| CN102919510A (en) * | 2012-11-29 | 2013-02-13 | 南京财经大学 | Preparation method of rice bran selenoprotein powder |
| CN102919510B (en) * | 2012-11-29 | 2013-09-04 | 南京财经大学 | Preparation method of rice bran selenoprotein powder |
| CN103461646A (en) * | 2013-09-29 | 2013-12-25 | 东北农业大学 | Method suitable for removing arsenic from rice bran |
| CN103519196A (en) * | 2013-10-21 | 2014-01-22 | 江苏丹绿食品股份有限公司 | Production technology of rice bran dietary fiber |
| CN103549110A (en) * | 2013-11-04 | 2014-02-05 | 中南林业科技大学 | Method for extracting water-soluble rice bran proteins |
| CN103549110B (en) * | 2013-11-04 | 2015-06-24 | 中南林业科技大学 | Method for extracting water-soluble rice bran proteins |
| CN104178336A (en) * | 2014-08-29 | 2014-12-03 | 邹平健源油脂有限公司 | Preparation method of millet bran oil |
| CN104397339A (en) * | 2014-11-28 | 2015-03-11 | 江苏康之源粮油有限公司 | Manufacturing method of degreased rice bran |
| CN104830927A (en) * | 2015-04-14 | 2015-08-12 | 无锡群硕谷唐生物科技有限公司 | Method for preparing ferulic acid oligosaccharide syrup by using wheat bran |
| CN104830927B (en) * | 2015-04-14 | 2018-03-23 | 无锡群硕谷唐生物科技有限公司 | A kind of method that forulic acid oligosaccharide syrup is prepared using wheat bran |
| CN105400857A (en) * | 2015-12-12 | 2016-03-16 | 常州大学 | Method for extracting antioxidative peptide from degreased rice bran |
| CN106561969A (en) * | 2016-10-18 | 2017-04-19 | 大连理工大学 | Technical method for extracting proteins from rice bran meal through repeated freezing and thawing of auxiliary weak alkali |
| CN107988297A (en) * | 2017-11-27 | 2018-05-04 | 丸美化妆品株式会社 | The application of a kind of preparation method and vinasse small-molecular peptides of vinasse small-molecular peptides in skin care item |
| CN107988297B (en) * | 2017-11-27 | 2021-08-10 | 丸美化妆品株式会社 | Preparation method of vinasse small molecular peptide and application of vinasse small molecular peptide in skin care product |
| CN108208506A (en) * | 2018-01-18 | 2018-06-29 | 浙江省柑桔研究所 | A kind of millet cavings beverage and preparation method thereof |
| CN108850550A (en) * | 2018-06-25 | 2018-11-23 | 天柱县森源米业有限责任公司 | A kind of defatted rice bran multi-strain fermentation product |
| CN109602660A (en) * | 2019-01-17 | 2019-04-12 | 上海灰灵生物科技有限公司 | A kind of cosmetic formulations and preparation method thereof rich in Antioxidative Factors |
| CN111440012A (en) * | 2020-04-16 | 2020-07-24 | 上海农乐生物制品股份有限公司 | Preparation method of low-chlorine amino acid fertilizer |
| CN112244297A (en) * | 2020-10-24 | 2021-01-22 | 山西大学 | Preparation method and application of furfuryl alkali extract |
| CN112481022A (en) * | 2020-12-22 | 2021-03-12 | 霍邱县敏东米业有限公司 | Method for synchronously preparing rice bran oil and high-purity active peptide by using rice bran |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102115690B (en) | 2013-06-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102115690B (en) | Method for comprehensively utilizing rice bran | |
| CN102174627B (en) | Method for preparing rapeseed bioactive peptide | |
| CN102010460B (en) | Tea seed polypeptide and preparation method thereof | |
| CN102174626B (en) | Method for preparing corn peptide | |
| KR20060097947A (en) | Method for abstract of liquid extract from chlorella | |
| CN101525306A (en) | Method for extracting and separating natural taurine from octopus leftovers | |
| CN110684128B (en) | Method for extracting and refining polygonatum sibiricum polysaccharide | |
| CN102406048B (en) | Method for preparing sea cucumber glycoprotein by using sea cucumber blanching solution | |
| CN101869169B (en) | Method for preparing fish oligopeptide from gurry by combining fermentation and membrane technology | |
| CN102334586B (en) | Method for preparing tea leaf protein emulsifier from tea leaves | |
| CN107011457B (en) | A method of extracting preparation non-starch polysaccharide and small molecule nutrient molecule from sweet potato waste water | |
| CN106046188A (en) | Method for preparing fucoidin | |
| CN102020856B (en) | Method for recovering glucomannan and protein in konjac flying powder by enzyme process | |
| CN114457132A (en) | Method for preparing starch and non-thermal denatured protein powder by using rice as raw material | |
| CN109385414B (en) | Purification method of bromelain | |
| CN102453107A (en) | Method for preparing pectin from orange peels | |
| CN101095454B (en) | Method of separating protein from cereal kernel by adopting membrane separation technique | |
| CN102391117B (en) | Method for preparing chlorogenic acid from eucommia leaves | |
| CN107252117A (en) | A kind of method that oligopeptide, polysaccharide and dietary fiber are produced by raw material of peanut meal | |
| CN113481259B (en) | Method for extracting starch syrup and rice protein isolate by using broken rice | |
| CN100415697C (en) | Agent of extracting polyphenol from rape seed cakes or husks and its preparation | |
| JP7423803B2 (en) | Process method for efficiently producing carnosine-rich compounds | |
| CN104557578A (en) | Improved valine extraction process through microbial fermentation and method for preparing foliar fertilizer | |
| CN101289394A (en) | Process for abstracting chlorogenic acid and separating protein and small peptide form sunflower meal | |
| CN102174628B (en) | Preparation method of peanut bioactive peptides |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: WUHAN BAIXIN ZHENGYUAN BIOTECHNOLOGY ENGINEERING C Free format text: FORMER OWNER: WUHAN BAIXIN FOOD CO., LTD. Effective date: 20130609 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20130609 Address after: 430000, Hubei, Wuhan, East and West Lake five ditch West, innovation Avenue South Patentee after: WUHAN PASHUN ZHENGYUAN BIOTECH AND ENGINEERING CO., LTD. Address before: Huang Jia Da Wan 430000 Hubei Moshan District of Hongshan City, Wuhan Province Patentee before: Wuhan Baixin Food Co., Ltd. |