CN101575620A - Method for producing fermentation raw material, rice bran oil and protein feed by comprehensively utilizing rice bran - Google Patents
Method for producing fermentation raw material, rice bran oil and protein feed by comprehensively utilizing rice bran Download PDFInfo
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Abstract
The invention discloses a method for producing fermentation raw material, rice bran oil and protein feed by comprehensively utilizing rice bran, comprising the following steps of: 1) taking the rice bran as raw materials, adding 2-5 times of water, adjusting the pH as 5.0-7.0, adding an amylase, keeping the temperature within 105-110 DEG C for 4-6min, reducing the temperature to 93-97 DEG C and keeping the temperature for 0.5-1.5h; adjusting the pH to 4.0-4.4, adding a glucoamylase for saccharification at the temperature of 55-65 DEG C for 20-40h, filtering or centrifuging, taking the supernatant as the carbon sources of the raw material used for the fermentation of straight fermentation biology, and obtaining the biochemical product by fermentation; 2) drying the residue at the temperature of 60-105 DEG C, extracting the grease by a solvent method or a press-filtering method, and obtaining the rice bran oil; and 3) hydrolyzing the residue with the oil extracted to prepare the protein hydrolysis liquid for the nitrogen source of the fermentation, or also extracting the protein or taking the protein as the protein feed directly. The method plays an important role in the comprehensive utilization of the rice bran and is suitable for wide generation and application.
Description
Technical field
The present invention relates to agricultural cheap by product Comprehensive Utilization of Rice Bran method, particularly relate to a kind of method of producing biological fermentations such as lactic acid, ethanol, butyric acid, butanols with rice bran with raw material, Rice pollard oil and protein fodder.
Background technology
Rice bran is the by product in a kind of brown rice mill processes, and content accounts for 8% of brown rice greatly, and according to the food and agriculture organization statistics, Chinese rice output in 2007 is 1.85 hundred million tons (IRRI, 2009), therefore, and 0.148 hundred million ton of rice bran of the annual approximately production of China.Owing to contain the environmental pollutant in various enzymes and the mill processes in the rice bran, its very fast meeting souring corruption.Therefore, most of rice bran can cheap at once the selling as feed or fertilizer of quilt.Although there are these shortcomings in rice bran,, therefore, can adopt rational technology to carry out comprehensive utilization and be used for improving its added value because rice bran contains abundant nutrition (sugared 33.5-53.5%, protein 11.5-17.2%, grease 12.8-22.6%).
Most investigator emphasizes for the extraction of Rice pollard oil and rice bran protein and utilization, and seldom relates to for the utilization of starch in Comprehensive Utilization of Rice Bran and the rice bran.
Wherein, relevant Rice pollard oil extracts mainly contains following patents: application number is in 200610096767.4 the Chinese patent, be earlier with rice bran through microwave treatment, utilize the supercritical carbon dioxide extraction Rice pollard oil again.This method is to the equipment requirements harshness, the extraction cost height.In the Chinese patent of application number 02156713.1, be the top that the carbon of perfect combustion is placed on living rice bran, produce rice bran by smouldering.This method oil yield is low, and the wasting of resources is many, and there is certain pollution in atmosphere.The patent No. is in 02156713.1 the Chinese patent, is that the material to expanded mistake in time squeezes system oil.This method requires high to rice bran, and regulates difficult control.Application number is in 200810162034.5 the Chinese patent, to have proposed a kind of phytic acid ca of removing earlier in the rice bran, extracts the method for Rice pollard oil again.This method is soaked dried rice bran earlier, removes phytic acid ca, oven dry again, and then extract grease.Whole process energy consumption is inevitable very big, and does not propose the scheme of utilizing of last residue.
Wherein following: as to be in 200810007229.2 the Chinese patent application by the application number of Korea S DJ First sugar refining Corporation application about mainly containing of extracting of rice bran protein, a kind of method that is prepared protein extract by rice bran has been proposed, this method is by also dry with the rice bran granulation, carry out degreasing then, extract protein after the degreasing.Application number is in 200810047556.0 the Chinese patent application, to have proposed a kind of the slurry with thin albumen and extracted proteic technology in the rice bran.Water consumption is huge in this technology, handles 1 ton of rice bran and approximately will produce waste water more than 23 tons, is rich in composition such as water soluble amino-acid, salt in the rice bran in this waste water, will cause severe contamination to environment.Even handle, processing costs is also with huge, and is therefore relatively poor from the commerce feasibility.
Wherein, about the following patent documentations that mainly contain of rice bran comprehensive utilization: announced among the CN 101220314A that a kind of screw rod pressing method of using obtains Rice pollard oil, pulverizes the method that obtains half defatted rice bran protein powder to squeeze cake then.The method oil yield that oil is carried in the squeezing of this method is lower, and protein content can not reach the standard of protein fodder less than 40% in the half defatted rice bran protein powder after the oil removing, and contain some fat and lipase, caused and become sour, can only very cheap fast sale.Among the CN101301067A (application number is 200810062415.6), proposed a kind of method, obtained rice bran protein, rice bran polysaccharide, phytic acid and Rice pollard oil respectively by the whole bag of tricks in this method with actives in the comprehensive utilization rice bran.But almost account for the method for utilizing of rice bran starch about 50% in this method and in the not mentioned rice bran.Among the Chinese patent CN1180710C, a kind of method for preparing natural nutrient food with rice bran has been proposed.This method is produced hydrolyzed solution with amylase and proteolytic enzyme compound action then by using earlier the normal hexane extraction Rice pollard oil, adds the method that Rice pollard oil and other nutritive ingredient are taken composite nutritive food in hydrolyzed solution.For rice bran, be that a kind of composition is not highly stable agricultural waste, simply difficult to obtain the more stable food of quality by above technology.
In sum, all mainly be directly rice bran to be carried out protein or greasy extraction in the method for present rice bran utilization.On the one hand, because the rice bran starch content is higher,, improved the cost that extracts so the rice bran amount that albumen of being measured equally or grease need be handled increases.On the other hand, contain more impurity in the rice bran in the water soluble component, can impact, further increased extraction cost albumen or greasy extraction efficiency.For rice bran, be that a kind of composition is not very stable agricultural waste, difficult by simple technology to obtain the more stable food of quality.
Rice pollard oil is a kind of oil that extracts from rice bran, it not only contains more rich multivitamin than other edible oils, and saturated acid in the oil: monoenoic acid: the ratio of polyenoic acid is 1: 1: 1, and this natural ratio is near the ratio standard of Food and Argriculture OrganizationFAO and World Health Organization's proposition.If before spoiled by rancid oil or fat, grease is extracted, just can be used as the Rice pollard oil of use.Even Rice pollard oil has taken place to become sour, the grease that extract this moment still can be used for the production biofuel, is used as fuel.
Fermentation industry, especially large type biological fermentation raw material, in the fermentative production as products such as lactic acid, ethanol, butyric acid, butanols, raw materials cost is huge for the influence of product competitiveness.In early days, main source of carbon is glucose, starch crops (W-Gum, wheat starch, potato starch), sugarcane etc.; What nitrogenous source mainly used is yeast powder.In order to reduce cost, the carbon of multiple cheapness, nitrogenous source are used to fermentation.Carbon source mainly contains farm crop (corn, barley, wheat, rice), starch crops (tapioca (flour)), whey powder, molasses, sweet sorghum, beet etc.; Nitrogenous source mainly contains corn steep liquor, dregs of beans, farm crop albumen (wheat plantule protein, dregs of beans, cottonseed protein) etc.But adopting the rice bran hydrolysis sugar is carbon source, and adopting the rice bran protein hydrolyzed solution simultaneously is that the research that nitrogen source fermentation is produced L-lactic acid does not appear in the newspapers.
L-lactic acid is a kind of important industrial raw material, can be widely used in medicine, food, chemical industry, brewages, multiple industry such as spices, leather, cigarette and printing and dyeing.In recent years, along with the mass consumption of " white pollution ", fossil resource and climbing up and up of Nonrenewable resources price, the substitute products of the friendly type of development environment are the inevitable requirements that guarantees ecological chain benign cycle, sustainable economic development.In numerous biodegradable polymers, poly(lactic acid) has won attracting attention of global plastic industry with its excellent mechanical property, wide application field, remarkable social benefit.With lactic acid is that raw material is produced poly(lactic acid), causes the extensive attention of countries in the world as Biodegradable material, is considered to one of macromolecular material of tool development prospect.Global plastics consumption was about 1.15 hundred million tons in 2000, if behind the 10-20 in the global range bio-based plastics can substitute the 10-20% of petroleum-based plastics consumption, the annual requirement of poly(lactic acid) will reach ten thousand tons of 1150-2300.It is exactly that cost is too high that the production of L-lactic acid at present runs into maximum problem, carbon source has occupied the significant proportion of production cost at present, select a kind of raw material of cheapness to replace the production cost that currently used starch not only can reduce L-lactic acid, and have plan and advance effect for reducing industrial grain consumption.
Along with the exploitation of oil, energy dilemma highlights.Alcohol fuel and butanols are to cause two kinds of fuel of showing great attention in the world, and ethanol uses for many years in the country of Brazil, the U.S., and China just just began in nearly 2 years.Brazil is raw material with sugarcane; the U.S. and China are raw material with W-Gum just mostly; along with the appearance of crisis in food in 2008, will no longer be advocated with the approach of the large type chemical of grain-production, it is the raw material production alcohol fuel that present COFCO has begun to adopt tapioca (flour).Fully utilize and produce these biofuels and do not appear in the newspapers so far as raw material with this agricultural cheap by product of rice bran.
Summary of the invention
The invention provides a kind of comprehensive utilization rice bran with low cost and produce the method for biological fermentations such as lactic acid, ethanol, butyric acid, butanols with raw material, Rice pollard oil and protein fodder.
The method of comprehensive utilization rice bran provided by the present invention is to be used for forming a complete production network the biological fermentation of lactic acid, ethanol, butyric acid, butanols etc. with raw material, Rice pollard oil and protein fodder, can may further comprise the steps:
1) with the rice bran be raw material, add 2-5 water doubly, adjusting pH is 5.0-7.0, adds amylase, and 105-110 ℃ kept 4-6 minute, cools to 93-97 ℃ of insulation 0.5-1.5 hour; Adjusting pH is 4.0-4.4, adds saccharifying enzyme, 55-65 ℃ saccharification 20-40 hour, filter or centrifugal, the enzymic hydrolysis supernatant liquor is used for fermentation and obtains the biochemical industry product directly as the carbon source and the nutrition of biological fermentation with raw material, filtration residue A is standby;
2) with residue A 60-105 ℃ of oven dry, adopt solvent method or filter press technique to extract grease, obtain Rice pollard oil;
3) with carrying that residue B behind the oil extracts albumen or directly as protein fodder.
Wherein, the rice bran in the described step 1) is yellow chaff, white chaff or both mixtures; Described biochemical industry product is L-lactic acid, ethanol, butanols or butyric acid; Described diastatic addition is 1～10mL/kg raw material, and the addition of saccharifying enzyme is 1～10mL/kg.
In the above method, can get a part of acid hydrolysis of the filtration residue A in the described step 1), filter, the hydrolysis supernatant liquor is a protein hydrolyte, as biological fermentation with raw material nitrogenous source, be used for fermentation and obtain the biochemical industry product; Described acid hydrolysis condition is: add 1.5% (w/w) sulfuric acid or hydrochloric acid of 4 times of volumes, hydrolysis temperature is 70～120 ℃, hydrolysis 20～30 hours.
Described step 2) extracts grease with solvent method in, method is: with residue A with cylinder under 60-105 ℃, dry to moisture below 10%, weight ratio with feed liquid is 2.5-6: 1 solvent soaking, stirred 4-8 hour down at temperature 35-65 ℃, filtration obtains filtrate and residue, sherwood oil is removed in the filtrate distillation, obtains crude rice bran oil.Described solvent is normal hexane, sherwood oil or anhydrous diethyl ether.
The biological fermentation that obtains with aforesaid method also belongs to protection scope of the present invention with raw material, Rice pollard oil and protein fodder.Wherein, rice bran biological fermentation raw material is rice bran enzymic hydrolysis supernatant liquor and/or the enzymic hydrolysis residue albumen supernatant liquor that obtains of hydrolysis again.
It also is one of purpose of the present invention with the application of raw material in preparation biochemical industry product that this rice bran biological fermentation is provided, wherein, described rice bran enzymic hydrolysis supernatant liquor is the carbon source and the nutrition of biological fermentation raw material, and described albumen supernatant liquor is the nitrogenous source of biological fermentation raw material.
The invention provides with rice bran and produce the method for biological fermentation with raw material, Rice pollard oil and protein fodder.This method is to utilize the starch in the enzymatic hydrolysis rice bran to be converted into sugar, utilizes sugar in the supernatant liquor and nutrition as biochemical industry products such as fermentation raw material lactic acid producing in next life, ethanol, butanols, butyric acid.Residue behind the hydrolysis sugar extracts grease, carries residue behind the oil as protein fodder, thereby reaches the purpose of comprehensive utilization rice bran.Because grease and the content of albumen own in the rice bran are not high, if directly extract, extraction cost is higher.And with amylase the starch in the rice bran is hydrolyzed to sugar earlier among the present invention, and filter then, so just water soluble component in the rice bran and starch are all removed.At this moment, the 2-3 that grease and Protein content can be brought up to rice bran in the residue doubly, can reduce extraction cost (extracting energy consumption and material consumption obviously reduces) so on the one hand when extracting grease and protein, the carbon source in the rice bran is reasonably used on the other hand.The present invention will play a significant role in Comprehensive Utilization of Rice Bran, be fit to extensive promotion and application.
Below in conjunction with specific embodiment the present invention is described in further details.
Description of drawings
Fig. 1 is a kind of synoptic diagram of producing L-lactic acid, Rice pollard oil and protein fodder method with rice bran of the present invention
Fig. 2 another kind of the present invention is produced the synoptic diagram of L-lactic acid, Rice pollard oil and protein fodder method with rice bran
Embodiment
Method therefor is ordinary method if no special instructions among the following embodiment.
Of the present inventionly provide a kind of novel method that fully utilizes rice bran.The technology that utilization is optimized successively extracts starch, grease and the albumen in the rice bran.Reduce the cost of whole leaching process simultaneously, made full use of Biological resources, reduced the generation of the waste pollution of production process, produced higher economic benefit.
The method of comprehensive utilization rice bran provided by the invention, be after starch in the enzymic hydrolysis rice bran is converted into sugar, (contain the sugar that water soluble component and starch hydrolysis obtain in the rice bran with supernatant liquor, can be used as carbon source and nutrition) and hydrolysis sugar after residue (residue A) again hydrolysis posthydrolysis liquid (contain protolysate, can be used as nitrogenous source) as biochemical industry products such as raw material lactic acid producing in next life, ethanol, butanols, butyric acid.Then, utilize the residue (residue A) behind the hydrolysis sugar to extract grease, carry that residue (residue B) behind the oil can be used for extracting albumen or directly as protein fodder.Concrete application is as follows:
The method of comprehensive utilization rice bran provided by the present invention can may further comprise the steps: referring to shown in Figure 1.
1) add 2-5 water doubly in the rice bran, adjusting pH is 5.0-7.0 (being preferably 6.0), adds amylase, and 105-110 ℃ (being preferably 108 ℃) keeps 4-6 minute (being preferably 5 minutes), cools to 0.5-1.5 hour (being preferably 1 hour) of 93-97 ℃ of insulation; Adjusting pH is 4.0-4.4 (being preferably 4.2), add saccharifying enzyme, 55-65 ℃ (being preferably 60 ℃) saccharification 20-40 hour, filtration or centrifugal, supernatant liquor obtains the biochemical industry product as the carbon source substratum and the nutrition of direct fermentation production biochemical industry product through fermentation; (residue A) is standby for filtration residue;
2) with residue (residue A) 60-105 ℃ oven dry, adopt solvent method or filter press technique to extract grease, obtain Rice pollard oil;
3) with carrying that residue (residue B) behind the oil extracts albumen or directly as protein fodder.
Produce biological fermentation with in the method for raw material, Rice pollard oil and protein fodder above-mentioned with rice bran, the rice bran in the described step 1) is yellow chaff, white chaff or both mixtures.
Biological fermentation in the described step 1) can be used for the production of biochemical industry products such as lactic acid, ethanol, butanols or butyric acid with raw material.
Diastatic addition is 1～10mL/kg raw material in the described step 1), and the addition of saccharifying enzyme is 1～10mL/kg raw material.
Describedly extract with solvent method that greasy method is among the residue A: with residue A with cylinder 60-105 ℃ of oven dry (moisture is below 10%) down, weight ratio with feed liquid is 2.5-6: 1 solvent (normal hexane, sherwood oil or anhydrous diethyl ether) soaks, stirred 4-8 hour down at temperature 35-65 ℃, filter and obtain filtrate and residue B.Sherwood oil is removed in filtrate distillation, obtains crude rice bran oil, and residue B can be used for extracting albumen or directly as protein fodder.
The method of another kind of comprehensive utilization rice bran provided by the present invention is referring to shown in Figure 2.Different with Fig. 1 is, the part of the filtration residue in the step 1) (residue A) is used for acid hydrolysis, filters, and supernatant liquor is a protein hydrolyte, as the nitrogenous source substratum of direct fermentation production biochemical industry product, can be used for the fermentative production of biochemical industry product; Described acid hydrolysis condition is: add 1.5% (w/w) sulfuric acid or hydrochloric acid of 4 times of volumes, hydrolysis temperature is 70～120 ℃, hydrolysis 20～30 hours.
Another part enzymic hydrolysis residue A is again by described step 2) and 3) fully utilize.
Below further specify the present invention with specific embodiment.Those of ordinary skill in the art can expect some identical, replacement schemes apparently according to content disclosed by the invention, all should belong to disclosure of the present invention.Method therefor is ordinary method if no special instructions among the embodiment.
Embodiment 1, produce the L-lactic fermentation with raw material, Rice pollard oil and protein fodder with rice bran
As shown in Figure 1, obtain L-lactic fermentation raw material, Rice pollard oil and protein fodder with rice bran, concrete grammar may further comprise the steps:
1) gets white chaff 1kg, the tap water that adds 5 times (w/w), regulate pH and be 6.0 (5.0-7.0 all can), add 1mL amylase (available from Novi's letter), 108 ℃ (105-110 ℃ all can) keeps 5 minutes (4-6 minute all can), finishes liquefaction after cooling to 95 ± 2 ℃ of insulations 1 hour (0.5-1.5 hour all can) then.Regulate pH and be 4.2 (4.0-4.4 all can), add 1mL saccharifying enzyme (available from Novi's letter), 60 ℃ of (55-65 ℃ all can) saccharification 20 hours.Saccharification is finished after-filtration and is obtained supernatant liquor, and recording wherein, glucose content is 0.45kg.Produce L-lactic acid with this as fermenting raw materials, fermentation condition is: inoculation lactobacillus rhamnosus (CGMCC No.1.2134), and inoculum size is 10% (v/v), 42 ℃ fermented 48 hours, measured fermented liquid and determined to contain L-lactic acid 0.34kg.
2) 105 ℃ of filtration residues (residue A) (60-105 ℃ all can) oven dry is obtained 0.3kg, extract grease with solvent method then, method is: residue A is dried (moisture is below 10%) with cylinder down at 60-105 ℃, weight ratio with feed liquid is 2.5-6: 1 solvent normal hexane (normal hexane, sherwood oil or anhydrous diethyl ether all can) soaks, stirred 4-8 hour down at temperature 35-65 ℃, filter and obtain filtrate and residue B.Sherwood oil is removed in the filtrate distillation, obtains crude rice bran oil 0.084kg.
3) the 0.21kg residue of carrying behind the oil (residue B) can extract albumen.Method is: add 8 times water (w/v) among the residue B after the de-oiling, regulating pH with 10M NaOH is 11,38 ℃ of water-baths 150 minutes, 5000 rev/mins, centrifugal 15 minutes, collect supernatant liquor, is 5,5000 rev/mins with 5M hydrochloric acid with the pH regulator of supernatant liquor, and collecting precipitation is rice bran protein matter.
The residue B that carries behind the oil also can not do any processing directly as protein fodder.
Embodiment 2, produce biological fermentation with raw material and L-lactic acid, Rice pollard oil and protein fodder with rice bran
As shown in Figure 1, produce biological fermentation raw material and L-lactic acid, Rice pollard oil and protein fodder with rice bran, concrete grammar may further comprise the steps:
1) get yellow chaff 1kg, add the tap water of 2 times (w/w), regulating pH is 5.0, adds 1ml amylase, and 105 ℃ kept 5 minutes, cools to 95 ± 2 ℃ of insulations then and finishes liquefaction after 0.5 hour, and regulating pH is 4.0, adds the 2ml saccharifying enzyme, 55 ℃ of saccharification 40 hours.Saccharification is finished after-filtration and is obtained supernatant liquor, wherein glucose content is 0.4kg, with this as raw material production L-lactic acid, fermentation condition is: inoculation lactobacillus rhamnosus (CGMCCNo.1.2134), inoculum size is 10% (v/v), 42 ℃ fermented 48 hours, measured in the fermented liquid and contained L-lactic acid 0.35kg.
2) 105 ℃ of oven dry of filtration residue (residue A) are obtained 0.30kg, extract grease with solvent method then, solvent is a sherwood oil, and concrete grammar is identical with embodiment 1, obtains grease 0.085kg.
3) carry that 0.21kg residue (residue B) behind the oil can extract albumen (extracting method is identical with embodiment 1) or directly as protein fodder.
Embodiment 3, produce biological fermentation with raw material and L-lactic acid, Rice pollard oil and protein fodder with rice bran
As shown in Figure 2, produce biological fermentation raw material and L-lactic acid, Rice pollard oil and protein fodder with rice bran, concrete grammar may further comprise the steps:
1) get rice bran (white chaff, each 0.5kg of yellow chaff) 1kg, add the tap water of 4 times (w/w), regulating pH is 7.0, adds 1mL amylase, and 110 ℃ kept 5 minutes, cooled to 95 ± 2 ℃ of insulations then and finished liquefaction after 1.5 hours.Regulating pH is 4.4, adds the 1mL saccharifying enzyme, 65 ℃ of saccharification 30 hours.Saccharification is finished after-filtration and is obtained supernatant liquor, and wherein glucose content is 0.37kg, and 60 ℃ of oven dry of residue (A) obtain 0.3kg.
The 0.03kg residue of getting wherein (residue A) carries out acid hydrolysis, and hydrolysising condition is: add 1.5% (w/w) sulfuric acid or hydrochloric acid of 4 times of volumes, hydrolysis temperature is 70～120 ℃, and hydrolysis 20～30 hours obtains hydrolyzed solution.
Hydrolyzed solution is added after the saccharification in the supernatant liquor co-fermentation produce L-lactic acid, fermentation condition is: inoculation lactobacillus rhamnosus (CGMCC No.1.2134), and inoculum size is 10% (v/v), 42 ℃ of fermentations 48 hours, fermented liquid is measured and is contained L-lactic acid 0.31kg.
2) remaining 0.27kg residue (residue A) is extracted grease with solvent method, solvent is an anhydrous diethyl ether, and concrete grammar is identical with embodiment 1, obtains grease 0.075kg.
3) carry that 0.19kg residue (residue B) behind the oil can extract albumen (extracting method is identical with embodiment 1) or directly as protein fodder.
Embodiment 4, produce biological fermentation with raw material, Rice pollard oil and protein fodder with rice bran
With reference to figure 1 flow process.Produce biological fermentation raw material and ethanol, butanols, butyric acid, Rice pollard oil and protein fodder with rice bran, concrete grammar may further comprise the steps:
1) get rice bran 1kg (white chaff, each 0.5kg of yellow chaff), add the tap water of 4 times (w/w), regulating pH is 6.0, adds 1mL amylase, and 108 ℃ kept 5 minutes, cooled to 95 ± 2 ℃ of insulations then and finished liquefaction after 1 hour.Regulating pH is 4.2, adds the 1mL saccharifying enzyme, 60 ℃ of saccharification 30 hours.Saccharification is finished after-filtration and is obtained supernatant liquor, and wherein glucose content is 0.37kg, and 105 ℃ of oven dry of residue obtain 0.3kg.
Add residue of soya (addition is 80g/L) in the supernatant liquor (being adjusted into glucose content is 50g/L) with saccharification after intact, inorganic salt (anhydrous magnesium sulfate 0.04g, potassium primary phosphate 1.20g) and phosphoric acid buffer (1mol/L sodium salt phosphoric acid buffer, with 1mol/L SODIUM PHOSPHATE, MONOBASIC and the preparation of 1mol/L disodium hydrogen phosphate dodecahydrate, pH6.3, addition is every bottle of 100mL/L) ferment, fermentation condition is: inoculation clostridium acetobutylicum (CGMCC No.1.132), inoculum size is 10% (v/v), 36 ℃ of leavening temperatures, fermented 120 hours, fermented liquid is measured, determined wherein to contain ethanol 120g, butanols 16.77g, butyric acid 29.7g.
2) remaining 0.27kg residue (residue A) extracts grease with solvent method, and solvent is an anhydrous diethyl ether, and concrete grammar is identical with embodiment 1, obtains grease 0.075kg.
3) carry that 0.19kg residue (residue B) behind the oil can extract albumen (extracting method is identical with embodiment 1) or directly as protein fodder.
Embodiment 5, produce biological fermentation with raw material and ethanol, Rice pollard oil and protein fodder with rice bran
With reference to figure 1 flow process.Produce biological fermentation with raw material and ethanol, Rice pollard oil and protein fodder with rice bran, concrete grammar may further comprise the steps:
1) get rice bran 1kg (white chaff, each 0.5kg of yellow chaff), add the tap water of 4 times (w/w), regulating pH is 6.0, adds 1mL amylase, and 108 ℃ kept 5 minutes, cooled to 95 ± 2 ℃ of insulations then and finished liquefaction after 1 hour.Regulating pH is 4.2, adds the 1mL saccharifying enzyme, 60 ℃ of saccharification 30 hours.Saccharification is finished after-filtration and is obtained supernatant liquor, and wherein glucose content is 0.37kg, and 105 ℃ of oven dry of residue obtain 0.3kg.
Hydrolyzed solution inoculation yeast saccharomyces cerevisiae (CGMCC No.2.2081) with saccharification after intact, inoculum size is 10% (v/v), ferments, and 30 ℃ of bottom fermentations 48 hours, measures in the fermented liquid and contains ethanol 157g.
2) remaining 0.27kg residue (residue A) extracts grease with anhydrous diethyl ether, and concrete grammar is identical with embodiment 1, obtains grease 0.075kg.
3) carry that 0.19kg residue (residue B) behind the oil can extract albumen (extracting method is identical with embodiment 1) or directly as protein fodder.
Claims (10)
1, a kind of method that fully utilizes rice bran production fermentation raw material, Rice pollard oil and protein fodder may further comprise the steps:
1) with the rice bran be raw material, add 2-5 water doubly, adjusting pH is 5.0-7.0, adds amylase, and 105-110 ℃ kept 4-6 minute, cools to 93-97 ℃ of insulation 0.5-1.5 hour; Adjusting pH is 4.0-4.4, adds saccharifying enzyme, 55-65 ℃ saccharification 20-40 hour, filter or centrifugal, the enzymic hydrolysis supernatant liquor is used for fermentation and obtains the biochemical industry product directly as the carbon source and the nutrition of biological fermentation with raw material, filtration residue A is standby;
2) with residue A 60-105 ℃ of oven dry, adopt solvent method or filter press technique to extract grease, obtain Rice pollard oil;
3) with carrying that residue B behind the oil extracts albumen or directly as protein fodder.
2, method according to claim 1 is characterized in that: the rice bran in the described step 1) is yellow chaff, white chaff or both mixtures.
3, method according to claim 1 is characterized in that: the biochemical industry product in the described step 1) is L-lactic acid, ethanol, butanols or butyric acid.
4, method according to claim 1 is characterized in that: diastatic addition is 1～10mL/kg raw material in the described step 1), and the addition of saccharifying enzyme is 1～10mL/kg.
5, according to the arbitrary described method of claim 1 to 4, it is characterized in that: get a part of acid hydrolysis of the filtration residue A in the described step 1), filter, the hydrolysis supernatant liquor is a protein hydrolyte, as biological fermentation with raw material nitrogenous source, be used for fermentation and obtain the biochemical industry product; Described acid hydrolysis condition is: add 1.5% (w/w) sulfuric acid or hydrochloric acid of 4 times of volumes, hydrolysis temperature is 70～120 ℃, hydrolysis 20～30 hours.
6, according to the arbitrary described method of claim 1 to 4, it is characterized in that: extract grease with solvent method described step 2), method is: with residue A with cylinder under 60-105 ℃, dry to moisture below 10%, weight ratio with feed liquid is 2.5-6: 1 solvent soaking, stirred 4-8 hour down at temperature 35-65 ℃, filtration obtains filtrate and residue, and sherwood oil is removed in the filtrate distillation, obtains crude rice bran oil.
7, method according to claim 6 is characterized in that: described solvent is normal hexane, sherwood oil or anhydrous diethyl ether.
8, the rice bran fermentation raw material that obtains with each described method of claim 1-7.
9, described according to Claim 8 rice bran fermentation raw material is rice bran enzymic hydrolysis supernatant liquor and/or the enzymic hydrolysis residue albumen supernatant liquor that obtains of hydrolysis again.
10, claim 8 or the 9 described rice bran fermentation raw materials application in preparation biochemical industry product, described rice bran enzymic hydrolysis supernatant liquor is the carbon source and the nutrition of biological fermentation raw material, described albumen supernatant liquor is the nitrogenous source of biological fermentation raw material.
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