CN112481022A - Method for synchronously preparing rice bran oil and high-purity active peptide by using rice bran - Google Patents
Method for synchronously preparing rice bran oil and high-purity active peptide by using rice bran Download PDFInfo
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- 241000209094 Oryza Species 0.000 title claims abstract description 94
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 94
- 235000009566 rice Nutrition 0.000 title claims abstract description 94
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 31
- 235000019774 Rice Bran oil Nutrition 0.000 title claims abstract description 28
- 239000008165 rice bran oil Substances 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 22
- 238000000605 extraction Methods 0.000 claims abstract description 93
- 102000004190 Enzymes Human genes 0.000 claims abstract description 26
- 108090000790 Enzymes Proteins 0.000 claims abstract description 26
- 230000009849 deactivation Effects 0.000 claims abstract description 16
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 14
- 230000005684 electric field Effects 0.000 claims abstract description 14
- 239000002131 composite material Substances 0.000 claims abstract description 13
- 238000000194 supercritical-fluid extraction Methods 0.000 claims abstract description 12
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 7
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 7
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- 239000007788 liquid Substances 0.000 claims description 13
- 239000000463 material Substances 0.000 claims description 12
- 238000004108 freeze drying Methods 0.000 claims description 11
- 238000002386 leaching Methods 0.000 claims description 11
- 239000012528 membrane Substances 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 238000003756 stirring Methods 0.000 claims description 10
- 239000000284 extract Substances 0.000 claims description 7
- 238000001914 filtration Methods 0.000 claims description 7
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- 238000009210 therapy by ultrasound Methods 0.000 claims description 5
- 238000009777 vacuum freeze-drying Methods 0.000 abstract description 4
- 230000017854 proteolysis Effects 0.000 abstract description 3
- 230000006837 decompression Effects 0.000 abstract description 2
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- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
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- 238000011161 development Methods 0.000 description 2
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- 239000000796 flavoring agent Substances 0.000 description 2
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- OYHQOLUKZRVURQ-NTGFUMLPSA-N (9Z,12Z)-9,10,12,13-tetratritiooctadeca-9,12-dienoic acid Chemical compound C(CCCCCCC\C(=C(/C\C(=C(/CCCCC)\[3H])\[3H])\[3H])\[3H])(=O)O OYHQOLUKZRVURQ-NTGFUMLPSA-N 0.000 description 1
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- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 1
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 1
- 239000005642 Oleic acid Substances 0.000 description 1
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 1
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- 229930182558 Sterol Natural products 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- DTOSIQBPPRVQHS-PDBXOOCHSA-N alpha-linolenic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O DTOSIQBPPRVQHS-PDBXOOCHSA-N 0.000 description 1
- 235000020661 alpha-linolenic acid Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 235000021329 brown rice Nutrition 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 230000007365 immunoregulation Effects 0.000 description 1
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 1
- 238000010902 jet-milling Methods 0.000 description 1
- 229960004488 linolenic acid Drugs 0.000 description 1
- KQQKGWQCNNTQJW-UHFFFAOYSA-N linolenic acid Natural products CC=CCCC=CCC=CCCCCCCCC(O)=O KQQKGWQCNNTQJW-UHFFFAOYSA-N 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 1
- 235000021313 oleic acid Nutrition 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000000751 protein extraction Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000012958 reprocessing Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000003432 sterols Chemical class 0.000 description 1
- 235000003702 sterols Nutrition 0.000 description 1
- 238000003815 supercritical carbon dioxide extraction Methods 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/04—Pretreatment of vegetable raw material
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/025—Pretreatment by enzymes or microorganisms, living or dead
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
- C11B1/104—Production of fats or fatty oils from raw materials by extracting using super critical gases or vapours
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Microbiology (AREA)
- Health & Medical Sciences (AREA)
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- Zoology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
A method for synchronously preparing rice bran oil and high-purity active peptide from rice bran comprises screening rice bran to remove impurities, micronizing with air flow, and extracting with supercritical carbon dioxide to obtain rice bran oil; then the active peptide is prepared by composite proteolysis, high-voltage pulse electric field enzyme deactivation, ultrafiltration membrane filtration, vacuum decompression concentration and vacuum freeze drying. The invention adopts the method of superfine grinding and wall breaking combined with supercritical extraction to extract the rice bran oil, the extraction rate of the rice bran oil is higher, and no extraction solvent is left; the rice bran peptide is prepared by adopting composite proteolysis, high-voltage pulse electric field enzyme deactivation and vacuum freeze drying, so that the influence of high-temperature enzyme deactivation on the active peptide is avoided, and meanwhile, the purity of the active peptide is higher.
Description
Technical Field
The invention belongs to the technical field of deep processing of agricultural products, and relates to a method for synchronously preparing rice bran oil and high-purity active peptide by utilizing rice bran.
Background
The rice bran is a main byproduct of rice processing, and is a mixture of a skin layer removed during processing of brown rice into polished rice (white rice) and a small amount of rice germ and broken rice. The rice bran is mainly composed of components such as rice pericarp, seed coat, endosperm, aleurone layer, embryo, etc., and generally contains 15% of protein, 16-22% of fat, 3-8% of sugar, 10% of water, and about 125KJ/g of calories. Because the reprocessing difficulty of the rice bran is high and the cost is high, the rice bran is mostly used as feed at present.
The rice bran has high fat content. The rice bran oil prepared by squeezing or leaching rice bran is rich in unsaturated fatty acids such as oleic acid, linoleic acid, linolenic acid and the like, contains rich bioactive substances such as vitamin E, sterol, oryzanol and the like, has the effects of removing cholesterol in blood, reducing blood fat, promoting growth and development of human bodies and the like, and has excellent health-care efficacy and certain medicinal value.
The protein content of the rice bran is more than 1 time higher than that of the common polished rice, and the rice bran is a protein resource with great development potential. In recent years, with intensive research, it is found that polypeptides prepared from rice bran proteins have various bioactive functions such as oxidation resistance, blood pressure and blood lipid reduction, immunoregulation and the like. Rice bran peptide is considered to be a very promising functionally active peptide.
Disclosure of Invention
The invention aims to provide a method for synchronously preparing rice bran oil and high-purity active peptide by utilizing rice bran.
In order to achieve the above objects and other related objects, the present invention provides the following technical solutions: a method for synchronously preparing rice bran oil and high-purity active peptide by utilizing rice bran comprises the following steps:
step 1: screening rice bran to remove broken rice impurities to obtain impurity-removed rice bran;
step 2: pulverizing the impurity-removed rice bran to fineness of more than 300 meshes by adopting an airflow type ultrafine pulverizer to obtain ultrafine rice bran powder;
and step 3: putting the ultramicro rice bran powder into an extraction kettle of carbon dioxide supercritical extraction equipment, performing extraction operation, and respectively collecting an extraction product and extraction residues, wherein the extraction product is rice bran oil;
and 4, step 4: mixing the extraction residue and purified water according to the mass ratio of 1:5-10, then putting the mixed material into a leaching tank, and stirring for 4-6h at the stirring speed of 60-120 r/min; in the leaching process, starting ultrasonic treatment once every 10-15min, wherein the ultrasonic frequency is 25-35 KHz, and the power density is 0.25-0.50W/cm2The treatment time is 1.5-3.0min each time; after the extraction is finished, centrifuging at 3000-;
and 5: adding alkaline protease into the rice bran protein extract, keeping the pH of the solution at 7.0-8.0 and the temperature at 55 + -2 deg.C, and performing enzymolysis for 3-4 h; then adding flavourzyme, keeping the pH value of the solution at 7.0-8.0 and the temperature at 55 +/-2 ℃, and carrying out enzymolysis for 3-4 h;
step 6: putting the enzymolysis liquid obtained in the step (5) into high-voltage pulse electric field treatment equipment, wherein the electric field strength is 17.5-22.0kV/cm, the pulse frequency is 1500 Hz, and the treatment time is 5-10min, so as to obtain the composite protein enzymolysis liquid after enzyme deactivation;
and 7: cooling the composite protein enzymolysis liquid after enzyme deactivation to normal temperature, filtering by adopting an ultrafiltration membrane with the membrane aperture of 50-75nm and the operation pressure difference of 500-1000kPa, collecting filtrate to obtain enzymolysis ultrafiltrate, and then concentrating to 8-12% of the original volume to obtain enzymolysis concentrated solution;
and 8: placing the enzymolysis concentrated solution into a freeze drying device, and freeze drying at the temperature of minus 50 to minus 35 ℃ and under the vacuum gauge pressure of minus 0.095 to minus 0.100MPa until the water content of the material is lower than 5 to 8 percent to obtain the active peptide.
The preferable technical scheme is as follows: the extraction operation comprises the steps of putting the ultramicro rice bran powder into an extraction kettle of supercritical extraction equipment, and carrying out continuous extraction operation for 3 times to obtain an extraction product; the technological parameters of the first extraction are as follows: extracting under 25-30Mpa at 38.0 + -0.5 deg.C for 15-30 min; the technological parameters of the second extraction are as follows: extracting under 25-30Mpa at 43.0 + -0.5 deg.C for 15-30 min; the technological parameters of the third extraction are as follows: the extraction pressure is 30-35Mpa, the extraction temperature is 43.0 +/-0.5 ℃, and the extraction time is 15-30 min.
The preferable technical scheme is as follows: the enzyme activity of the alkaline protease is 10-20 ten thousand U, and the addition amount of the alkaline protease is 0.25-0.50% of the mass of the rice bran protein extracting solution; the enzyme activity of the flavourzyme is 10-20 ten thousand U, and the addition amount of the flavourzyme is 0.25-0.50% of the mass of the rice bran protein extracting solution.
Due to the application of the technical scheme, compared with the prior art, the invention has the advantages that:
1. the invention adopts jet milling and wall breaking to process the rice bran, which is beneficial to the subsequent preparation effect and efficiency of the rice bran oil and the active peptide.
2. The invention firstly extracts the grease in the rice bran by supercritical carbon dioxide, and then extracts and performs enzymolysis on the rice bran protein, thereby not only effectively extracting the grease, but also reducing the influence of the grease and oil-soluble substances on the protein extraction and enzymolysis.
3. The invention adopts the method of superfine grinding and wall breaking combined with supercritical extraction to extract the rice bran oil, the extraction rate of the rice bran oil is higher, and no extraction solvent residue exists.
4. The invention prepares the rice bran peptide by adopting composite proteolysis, high-voltage pulse electric field enzyme deactivation and vacuum freeze drying, avoids the influence of high-temperature enzyme deactivation on the active peptide, and simultaneously has higher purity of the active peptide.
Detailed Description
The following description of the embodiments of the present invention is provided for illustrative purposes, and other advantages and effects of the present invention will become apparent to those skilled in the art from the present disclosure.
Example 1: method for synchronously preparing rice bran oil and high-purity active peptide by using rice bran
A method for synchronously preparing rice bran oil and high-purity active peptide by utilizing rice bran comprises the following technical steps.
(1) Rice bran pretreatment
The rice bran material is the side product of glutinous rice, non-glutinous rice and indica rice and consists of mainly rice peel, seed coat, endosperm, aleurone layer, embryo and other components.
Removing impurities such as broken rice from rice bran by screening to obtain high-purity rice bran for use.
(2) Air flow ultramicro pulverization
And (3) pulverizing the high-purity rice bran in the step (1) to the fineness of more than 300 meshes by adopting airflow type superfine pulverizing equipment to obtain superfine rice bran powder for later use. The airflow type superfine crushing equipment adopts nitrogen as a gas source.
(3) Supercritical carbon dioxide extraction for preparing rice bran oil
And (3) putting the ultramicro rice bran powder in the step (2) into an extraction kettle of carbon dioxide supercritical extraction equipment, performing extraction operation, and respectively collecting an extraction product and residues for later use.
The extraction operation is to place the ultramicro bran powder in an extraction kettle of supercritical extraction equipment, and carry out continuous extraction operation for 3 times to obtain an extraction product; the technological parameters of the first extraction are as follows: extracting at 27Mpa at 38.5 deg.C for 25 min; the technological parameters of the second extraction are as follows: extracting at 28Mpa and 43.5 deg.C for 20 min; the technological parameters of the third extraction are as follows: extracting at 32Mpa and 43.5 deg.C for 25 min; collecting the extraction product for later use.
The extraction product is the prepared rice bran oil.
(4) Ultrasonic-assisted normal-temperature extraction of rice bran protein
Mixing the extraction residue in the step (3) and food-grade purified water according to the mass ratio of 1: 8; putting the mixed material into stirring and leaching equipment with an ultrasonic generating device, and keeping the normal temperature and the stirring rotating speed at 100 r/min; simultaneously, the leaching equipment starts ultrasonic treatment once every 12min, the ultrasonic frequency is 30 KHz, and the power density is 0.35W/cm2Each treatment time is 2 min; after the ultrasonic-assisted normal-temperature extraction is carried out for 5 hours, the mixed material is centrifuged at the rotating speed of 4000 r/min for 7 minutes, and the supernatant is collected, namely the rice bran protein extracting solution.
(5) Enzymolysis treatment of compound protein
Adding alkaline protease into the protein solution of the rice bran protein extract in the step (4), keeping the pH of the solution at 7.5 and the temperature at 55 ℃, and performing enzymolysis for 3.5 h; then adding flavourzyme, keeping the pH value of the solution at 7.5 and the temperature at 55 ℃, and carrying out enzymolysis for 3.5 h. The enzyme activity of the alkaline protease is 15 ten thousand U, and the addition amount of the alkaline protease is 0.35 percent of the mass of the protein solution. The enzyme activity of the flavor protease is 15 ten thousand U, and the addition amount of the flavor protease is 0.35 percent of the mass of the protein solution.
(6) Enzyme deactivation treatment by high-voltage pulse electric field
After the enzymolysis in the step (5) is finished, putting the solution into food high-voltage pulse electric field treatment equipment, wherein the electric field intensity is 20kV/cm, the pulse frequency is 1500 Hz, and the treatment time is 8min, so as to obtain the composite protein enzymolysis solution after enzyme deactivation for later use.
(7) Filtering and concentrating
And (4) filtering by using an ultrafiltration membrane. And (4) cooling the composite protein enzymolysis liquid after enzyme deactivation in the step (6) to normal temperature, filtering by adopting an ultrafiltration membrane with the membrane aperture of 60nm and the operating pressure difference of 800kPa, and collecting filtrate to obtain enzymolysis ultrafiltrate for later use.
Vacuum concentrating under reduced pressure. Placing the enzymolysis ultrafiltrate into vacuum decompression concentration equipment, boiling and concentrating to 10% of the original volume under the condition of vacuum gauge pressure of-0.095 MPa to obtain enzymolysis concentrated solution for later use.
(8) Vacuum freeze drying
Placing the enzymolysis concentrated solution in the step (7) in a freeze drying device, and freeze drying the enzymolysis concentrated solution at the temperature of-40 ℃ and the vacuum gauge pressure of-0.095 MPa until the water content of the material is lower than 5%; freeze drying to obtain the active peptide.
Example 2: method for synchronously preparing rice bran oil and high-purity active peptide by using rice bran
A method for synchronously preparing rice bran oil and high-purity active peptide by utilizing rice bran comprises the following steps:
step 1: screening rice bran to remove broken rice impurities to obtain impurity-removed rice bran;
step 2: pulverizing the impurity-removed rice bran to fineness of more than 300 meshes by adopting an airflow type ultrafine pulverizer to obtain ultrafine rice bran powder;
and step 3: putting the ultramicro rice bran powder into an extraction kettle of carbon dioxide supercritical extraction equipment, performing extraction operation, and respectively collecting an extraction product and extraction residues, wherein the extraction product is rice bran oil;
and 4, step 4: mixing the extraction residue and purified water according to the mass ratio of 1:5, then putting the mixed material into a leaching tank, and stirring for 4 hours at the stirring speed of 60 r/min; in the leaching process, ultrasonic treatment is started once every 10min, the ultrasonic frequency is 25KHz, and the power density is 0.25W/cm2Each treatment time is 1.5 min; after the extraction is finished, centrifuging at 3000r/min for 5min, and collecting supernatant, namely rice bran protein extracting solution;
and 5: adding alkaline protease into the rice bran protein extract, and performing enzymolysis for 3h at pH7.0 and 53 deg.C; then adding flavourzyme, keeping the pH value of the solution at 7.0 and the temperature at 53 ℃, and carrying out enzymolysis for 3 hours;
step 6: putting the enzymolysis liquid obtained in the step (5) into high-voltage pulse electric field treatment equipment, wherein the electric field intensity is 17.5kV/cm, the pulse frequency is 1500 Hz, and the treatment time is 5min, so as to obtain the composite protein enzymolysis liquid after enzyme deactivation;
and 7: cooling the composite protein enzymolysis liquid after enzyme deactivation to normal temperature, filtering by adopting an ultrafiltration membrane with the membrane aperture of 50nm and the operation pressure difference of 500kPa, collecting filtrate to obtain enzymolysis ultrafiltrate, and then concentrating to 8% of the original volume to obtain enzymolysis concentrated solution;
and 8: placing the enzymolysis concentrated solution in a freeze drying device, and freeze drying at-50 deg.C under-0.095 MPa until the water content of the material is less than 5% to obtain the active peptide.
The preferred embodiment is: the extraction operation comprises the steps of putting the ultramicro rice bran powder into an extraction kettle of supercritical extraction equipment, and carrying out continuous extraction operation for 3 times to obtain an extraction product; the technological parameters of the first extraction are as follows: extracting at 25Mpa and 37.5 deg.C for 15 min; the technological parameters of the second extraction are as follows: extracting at 25Mpa and 42.5 deg.C for 15 min; the technological parameters of the third extraction are as follows: the extraction pressure is 30Mpa, the extraction temperature is 420.5 ℃, and the extraction time is 15 min.
The preferred embodiment is: the enzyme activity of the alkaline protease is 10 ten thousand U, and the addition amount of the alkaline protease is 0.50 percent of the mass of the rice bran protein extracting solution; the enzyme activity of the flavourzyme is 10 ten thousand U, and the addition amount of the flavourzyme is 0.50 percent of the mass of the rice bran protein extracting solution.
Example 3: method for synchronously preparing rice bran oil and high-purity active peptide by using rice bran
A method for synchronously preparing rice bran oil and high-purity active peptide by utilizing rice bran comprises the following steps:
step 1: screening rice bran to remove broken rice impurities to obtain impurity-removed rice bran;
step 2: pulverizing the impurity-removed rice bran to fineness of more than 300 meshes by adopting an airflow type ultrafine pulverizer to obtain ultrafine rice bran powder;
and step 3: putting the ultramicro rice bran powder into an extraction kettle of carbon dioxide supercritical extraction equipment, performing extraction operation, and respectively collecting an extraction product and extraction residues, wherein the extraction product is rice bran oil;
and 4, step 4: mixing the extraction residue and purified water according to the mass ratio of 1:10, then putting the mixed material into a leaching tank, and stirring for 6 hours at the stirring speed of 120 r/min; in the leaching process, ultrasonic treatment is started once every 15min, the ultrasonic frequency is 35KHz, and the power density is 0.50W/cm2Each treatment time is 3.0 min; after the extraction is finished, centrifuging at 5000 r/min for 10min, and collecting supernatant, namely rice bran protein extracting solution;
and 5: adding alkaline protease into the rice bran protein extract, keeping the pH of the solution at 8.0 and the temperature at 57 ℃, and performing enzymolysis for 4 h; then adding flavourzyme, keeping the pH value of the solution at 8.0 and the temperature at 57 ℃, and carrying out enzymolysis for 4 hours;
step 6: putting the enzymolysis liquid obtained in the step (5) into high-voltage pulse electric field treatment equipment, wherein the electric field strength is 22.0kV/cm, the pulse frequency is 1500 Hz, and the treatment time is 10min, so as to obtain the composite protein enzymolysis liquid after enzyme deactivation;
and 7: cooling the composite protein enzymolysis liquid after enzyme deactivation to normal temperature, filtering by adopting an ultrafiltration membrane with the membrane aperture of 75nm and the operation pressure difference of 1000kPa, collecting filtrate to obtain enzymolysis ultrafiltrate, and then concentrating to 12% of the original volume to obtain enzymolysis concentrated solution;
and 8: placing the enzymolysis concentrated solution in a freeze drying device, and freeze drying at-35 deg.C under-0.10 MPa to water content of material below 8% to obtain active peptide.
The preferred embodiment is: the extraction operation comprises the steps of putting the ultramicro rice bran powder into an extraction kettle of supercritical extraction equipment, and carrying out continuous extraction operation for 3 times to obtain an extraction product; the technological parameters of the first extraction are as follows: the extraction pressure is 30Mpa, the extraction temperature is 38.5 ℃, and the extraction time is 30 min; the technological parameters of the second extraction are as follows: the extraction pressure is 30Mpa, the extraction temperature is 43.5 ℃, and the extraction time is 30 min; the technological parameters of the third extraction are as follows: the extraction pressure is 35Mpa, the extraction temperature is 43.5 ℃, and the extraction time is 30 min.
The preferred embodiment is: the enzyme activity of the alkaline protease is 20 ten thousand U, and the addition amount of the alkaline protease is 0.25 percent of the mass of the rice bran protein extracting solution; the enzyme activity of the flavourzyme is 20 ten thousand U, and the addition amount of the flavourzyme is 0.25 percent of the mass of the rice bran protein extracting solution.
The foregoing is illustrative of the preferred embodiment of the present invention and is not to be construed as limiting thereof in any way, and any modifications or variations thereof that fall within the spirit of the invention are intended to be included within the scope thereof.
Claims (3)
1. A method for synchronously preparing rice bran oil and high-purity active peptide by utilizing rice bran is characterized by comprising the following steps: comprises the following steps:
step 1: screening rice bran to remove broken rice impurities to obtain impurity-removed rice bran;
step 2: pulverizing the impurity-removed rice bran to fineness of more than 300 meshes by adopting an airflow type ultrafine pulverizer to obtain ultrafine rice bran powder;
and step 3: putting the ultramicro rice bran powder into an extraction kettle of carbon dioxide supercritical extraction equipment, performing extraction operation, and respectively collecting an extraction product and extraction residues, wherein the extraction product is rice bran oil;
and 4, step 4: mixing the extraction residue and purified water according to the mass ratio of 1:5-10, then putting the mixed material into a leaching tank, and stirring for 4-6h at the stirring speed of 60-120 r/min; in the leaching process, starting ultrasonic treatment once every 10-15min, wherein the ultrasonic frequency is 25-35 KHz, and the power density is 0.25-0.50W/cm2The treatment time is 1.5-3.0min each time; after the extraction is finished, centrifuging at 3000-;
and 5: adding alkaline protease into the rice bran protein extract, keeping the pH of the solution at 7.0-8.0 and the temperature at 55 + -2 deg.C, and performing enzymolysis for 3-4 h; then adding flavourzyme, keeping the pH value of the solution at 7.0-8.0 and the temperature at 55 +/-2 ℃, and carrying out enzymolysis for 3-4 h;
step 6: putting the enzymolysis liquid obtained in the step (5) into high-voltage pulse electric field treatment equipment, wherein the electric field strength is 17.5-22.0kV/cm, the pulse frequency is 1500 Hz, and the treatment time is 5-10min, so as to obtain the composite protein enzymolysis liquid after enzyme deactivation;
and 7: cooling the composite protein enzymolysis liquid after enzyme deactivation to normal temperature, filtering by adopting an ultrafiltration membrane with the membrane aperture of 50-75nm and the operation pressure difference of 500-1000kPa, collecting filtrate to obtain enzymolysis ultrafiltrate, and then concentrating to 8-12% of the original volume to obtain enzymolysis concentrated solution;
and 8: placing the enzymolysis concentrated solution into a freeze drying device, and freeze drying at the temperature of minus 50 to minus 35 ℃ and under the vacuum gauge pressure of minus 0.095 to minus 0.100MPa until the water content of the material is lower than 5 to 8 percent to obtain the active peptide.
2. The method for simultaneously preparing rice bran oil and high purity active peptide from rice bran according to claim 1, wherein the steps of: the extraction operation comprises the steps of putting the ultramicro rice bran powder into an extraction kettle of supercritical extraction equipment, and carrying out continuous extraction operation for 3 times to obtain an extraction product; the technological parameters of the first extraction are as follows: extracting under 25-30Mpa at 38.0 + -0.5 deg.C for 15-30 min; the technological parameters of the second extraction are as follows: extracting under 25-30Mpa at 43.0 + -0.5 deg.C for 15-30 min; the technological parameters of the third extraction are as follows: the extraction pressure is 30-35Mpa, the extraction temperature is 43.0 +/-0.5 ℃, and the extraction time is 15-30 min.
3. The method for simultaneously preparing rice bran oil and high purity active peptide from rice bran according to claim 1, wherein the steps of: the enzyme activity of the alkaline protease is 10-20 ten thousand U, and the addition amount of the alkaline protease is 0.25-0.50% of the mass of the rice bran protein extracting solution; the enzyme activity of the flavourzyme is 10-20 ten thousand U, and the addition amount of the flavourzyme is 0.25-0.50% of the mass of the rice bran protein extracting solution.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102115690A (en) * | 2011-01-12 | 2011-07-06 | 武汉百信食品有限公司 | Method for comprehensively utilizing rice bran |
| CN103549110A (en) * | 2013-11-04 | 2014-02-05 | 中南林业科技大学 | Method for extracting water-soluble rice bran proteins |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102115690A (en) * | 2011-01-12 | 2011-07-06 | 武汉百信食品有限公司 | Method for comprehensively utilizing rice bran |
| CN103549110A (en) * | 2013-11-04 | 2014-02-05 | 中南林业科技大学 | Method for extracting water-soluble rice bran proteins |
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| 张焱: "米糠蛋白ACE抑制肽分离纯化工艺的研究" * |
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