CN109134691A - A kind of method of high efficiency extraction Polysaccharide in Pleurotus eryngii - Google Patents
A kind of method of high efficiency extraction Polysaccharide in Pleurotus eryngii Download PDFInfo
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- CN109134691A CN109134691A CN201811367187.3A CN201811367187A CN109134691A CN 109134691 A CN109134691 A CN 109134691A CN 201811367187 A CN201811367187 A CN 201811367187A CN 109134691 A CN109134691 A CN 109134691A
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- Prior art keywords
- pleurotus eryngii
- polysaccharide
- high efficiency
- papain
- powder
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
Abstract
The present invention relates to a kind of methods of high efficiency extraction Polysaccharide in Pleurotus eryngii, comprising the following steps: is 48-55 DEG C in temperature, pressure handles 3-6min under conditions of being 20-30MPa, and release obtains paste serous material after mixing Pleurotus eryngii powder with distilled water；Paste serous material is warming up to 48-56 DEG C, adjusting pH value is 5.5-6.5, lipase, papain and cellulase is added, after keeping the temperature 40-60min, ultrasonic wave extraction obtains extracting solution, filters, concentration, alcohol precipitation, centrifugation, takes precipitating, vacuum freeze drying obtains Polysaccharide in Pleurotus eryngii.The present invention extracts polysaccharide by using the method being ultrasonically treated in enzymolysis process, greatly increases the recovery rate of polysaccharide, which is up to 27.8%.
Description
Technical field
The invention belongs to technical field of polysaccharide extraction, in particular to a kind of method of high efficiency extraction Polysaccharide in Pleurotus eryngii.
Background technique
Pleurotus eryngii (Pleurotus eryngii) is also known as pleurotus eryngii, snow young pilose antler, is the one kind for introducing China's cultivation in recent years
High-end large-scale Rare edible fungus.Pleurotus eryngii is full of nutrition, is rich in protein, carbohydrate, vitamin and calcium, magnesium, copper, zinc
Equal minerals, can be improved immune function of human body, have the effects that anticancer, reducing blood lipid, ease constipation stomach and beauty to human body.
Polysaccharide is one of pharmaceutical component important in edible mushroom.The Polysaccharide in Pleurotus eryngii immunomodulator special as one kind,
It is mediated with strong host in activated T lymphocytes, antibody can be stimulated to be formed, enhance human immunity, play anticancer
Effect.Therefore it provides a kind of method of high efficiency extraction Polysaccharide in Pleurotus eryngii is particularly significant.
Summary of the invention
It is an object of the invention to: a kind of method of high efficiency extraction Polysaccharide in Pleurotus eryngii is provided.
In order to achieve the above-mentioned object of the invention, the invention provides the following technical scheme:
A kind of method of high efficiency extraction Polysaccharide in Pleurotus eryngii, comprising the following steps:
S1: it after fresh Pleurotus eryngii is cleaned up, cuts fritter and is put into vacuum oven drying, to straw mushroom perseverance quality, be put into
It is crushed in pulverizer, sieving obtains Pleurotus eryngii powder, spare；
S2: after the Pleurotus eryngii powder that step S1 is obtained is mixed with 10-20 times of Pleurotus eryngii powder weight of distilled water, in temperature
Degree is 48-55 DEG C, and pressure handles 3-6min under conditions of being 20-30MPa, and release obtains paste serous material；
S3: being warming up to 48-56 DEG C for paste serous material, and adjusting pH value is 5.5-6.5, be added lipase, papain and
After cellulase, after keeping the temperature 40-60min, in the case where supersonic frequency is 120-140kHz, ultrasonic wave extraction 4-7min, interval 8-
12min, in the case where supersonic frequency is 160-180kHz, ultrasonic wave extraction 4-7min obtains extracting solution；
S4: filtering extracting solution, and filtrate is compressed to the 1/4-1/3 of former filtrate volume, adds former pleurotus eryngii powder
The ethanol solution of the 75-78% of 12-18 times of last weight carries out alcohol precipitation, and alcohol analyses 16-19h, and centrifugation takes precipitating, and vacuum refrigeration is dry
It is dry, obtain Polysaccharide in Pleurotus eryngii.
Preferably, the partial size of Pleurotus eryngii powder is 100-120 mesh in step S1.
Preferably, the partial size of Pleurotus eryngii powder is 110 mesh in step S1.
Preferably, the addition total amount of lipase, papain and cellulase is Pleurotus eryngii powder in step S3
0.1-0.4%.
Preferably, the addition total amount of lipase, papain and cellulase is Pleurotus eryngii powder in step S3
0.2%.
Preferably, the mass ratio of lipase, papain and cellulase is 1:3.8-4.2:1.8- in step S3
2.2。
Preferably, the mass ratio of lipase, papain and cellulase is 1:4:2 in step S3.
The beneficial effects of the present invention are: the present invention is by using the method extraction being ultrasonically treated in enzymolysis process
Polysaccharide greatly increases the recovery rate of polysaccharide, which is up to 27.8%.
Specific embodiment
Embodiment 1
A kind of method of high efficiency extraction Polysaccharide in Pleurotus eryngii, comprising the following steps:
S1: it after fresh Pleurotus eryngii is cleaned up, cuts fritter and is put into vacuum oven drying, to straw mushroom perseverance quality, be put into
It is crushed in pulverizer, sieving obtains Pleurotus eryngii powder, spare；
S2: after the Pleurotus eryngii powder that step S1 is obtained is mixed with 10 times of Pleurotus eryngii powder weight of distilled water, in temperature
It is 48 DEG C, pressure handles 6min under conditions of being 20MPa, and release obtains paste serous material；
S3: paste serous material is warming up to 48 DEG C, adjusting pH value is 5.5-6.5, and lipase, papain and fiber is added
After plain enzyme, after keeping the temperature 60min, in the case where supersonic frequency is 120kHz, ultrasonic wave extraction 7min, interval 8min are in supersonic frequency
Under 160kHz, ultrasonic wave extraction 7min obtains extracting solution；
S4: filtering extracting solution, and filtrate is compressed to the 1/4 of former filtrate volume, adds former Pleurotus eryngii powder weight
78% ethanol solution of 12 times of amount carries out alcohol precipitation, and alcohol analyses 16h, and centrifugation takes precipitating, it is more to obtain Pleurotus eryngii for vacuum freeze drying
Sugar.
Wherein, the partial size of Pleurotus eryngii powder is 100 mesh in step S1.
The addition total amount of lipase, papain and cellulase is the 0.1% of Pleurotus eryngii powder in step S3.
The mass ratio of lipase, papain and cellulase is 1:3.8:1.8 in step S3.
Embodiment 2
A kind of method of high efficiency extraction Polysaccharide in Pleurotus eryngii, comprising the following steps:
S1: it after fresh Pleurotus eryngii is cleaned up, cuts fritter and is put into vacuum oven drying, to straw mushroom perseverance quality, be put into
It is crushed in pulverizer, sieving obtains Pleurotus eryngii powder, spare；
S2: after the Pleurotus eryngii powder that step S1 is obtained is mixed with 15 times of Pleurotus eryngii powder weight of distilled water, in temperature
It is 53 DEG C, pressure handles 4min under conditions of being 25MPa, and release obtains paste serous material；
S3: paste serous material is warming up to 52 DEG C, adjusting pH value is 5.5-6.5, and lipase, papain and fiber is added
After plain enzyme, after keeping the temperature 50min, in the case where supersonic frequency is 130kHz, ultrasonic wave extraction 6min, interval 10min are in supersonic frequency
Under 170kHz, ultrasonic wave extraction 6min obtains extracting solution；
S4: filtering extracting solution, and filtrate is compressed to the 1/3 of former filtrate volume, adds former Pleurotus eryngii powder weight
76% ethanol solution of 15 times of amount carries out alcohol precipitation, and alcohol analyses 18h, and centrifugation takes precipitating, it is more to obtain Pleurotus eryngii for vacuum freeze drying
Sugar.
Wherein, the partial size of Pleurotus eryngii powder is 110 mesh in step S1.
The addition total amount of lipase, papain and cellulase is the 0.2% of Pleurotus eryngii powder in step S3.
The mass ratio of lipase, papain and cellulase is 1:4:2 in step S3.
Embodiment 3
A kind of method of high efficiency extraction Polysaccharide in Pleurotus eryngii, comprising the following steps:
S1: it after fresh Pleurotus eryngii is cleaned up, cuts fritter and is put into vacuum oven drying, to straw mushroom perseverance quality, be put into
It is crushed in pulverizer, sieving obtains Pleurotus eryngii powder, spare；
S2: after the Pleurotus eryngii powder that step S1 is obtained is mixed with 20 times of Pleurotus eryngii powder weight of distilled water, in temperature
It is 55 DEG C, pressure handles 3min under conditions of being 30MPa, and release obtains paste serous material；
S3: paste serous material is warming up to 56 DEG C, adjusting pH value is 5.5-6.5, and lipase, papain and fiber is added
After plain enzyme, after keeping the temperature 40min, in the case where supersonic frequency is 140kHz, ultrasonic wave extraction 4min, interval 12min are in supersonic frequency
Under 180kHz, ultrasonic wave extraction 4min obtains extracting solution；
S4: filtering extracting solution, and filtrate is compressed to the 1/4 of former filtrate volume, adds former Pleurotus eryngii powder weight
78% ethanol solution of 18 times of amount carries out alcohol precipitation, and alcohol analyses 19h, and centrifugation takes precipitating, it is more to obtain Pleurotus eryngii for vacuum freeze drying
Sugar.
Wherein, the partial size of Pleurotus eryngii powder is 120 mesh in step S1.
The addition total amount of lipase, papain and cellulase is the 0.4% of Pleurotus eryngii powder in step S3.
The mass ratio of lipase, papain and cellulase is 1:4.2:2.2 in step S3.
Polysaccharide in Pleurotus eryngii recovery rate of the invention is up to 27.8%.
Claims (7)
1. a kind of method of high efficiency extraction Polysaccharide in Pleurotus eryngii, it is characterised in that: the following steps are included:
S1: it after fresh Pleurotus eryngii is cleaned up, cuts fritter and is put into vacuum oven drying, to straw mushroom perseverance quality, be put into crushing
It is crushed in machine, sieving obtains Pleurotus eryngii powder, spare；
S2: after the Pleurotus eryngii powder that step S1 is obtained is mixed with 10-20 times of Pleurotus eryngii powder weight of distilled water, it is in temperature
48-55 DEG C, pressure handles 3-6min under conditions of being 20-30MPa, and release obtains paste serous material；
S3: paste serous material is warming up to 48-56 DEG C, adjusting pH value is 5.5-6.5, and lipase, papain and fiber is added
After plain enzyme, after keeping the temperature 40-60min, in the case where supersonic frequency is 120-140kHz, ultrasonic wave extraction 4-7min, interval 8-12min,
In the case where supersonic frequency is 160-180kHz, ultrasonic wave extraction 4-7min obtains extracting solution；
S4: filtering extracting solution, and filtrate is compressed to the 1/4-1/3 of former filtrate volume, adds former Pleurotus eryngii powder weight
The ethanol solution of the 75-78% of 12-18 times of amount carries out alcohol precipitation, and alcohol analyses 16-19h, and centrifugation takes precipitating, vacuum freeze drying obtains
To Polysaccharide in Pleurotus eryngii.
2. a kind of method of high efficiency extraction Polysaccharide in Pleurotus eryngii according to claim 1, it is characterised in that: apricot Bao in step S1
The partial size at mushroom powder end is 100-120 mesh.
3. a kind of method of high efficiency extraction Polysaccharide in Pleurotus eryngii according to claim 1, it is characterised in that: apricot Bao in step S1
The partial size at mushroom powder end is 110 mesh.
4. a kind of method of high efficiency extraction Polysaccharide in Pleurotus eryngii according to claim 1, it is characterised in that: fatty in step S3
The addition total amount of enzyme, papain and cellulase is the 0.1-0.4% of Pleurotus eryngii powder.
5. a kind of method of high efficiency extraction Polysaccharide in Pleurotus eryngii according to claim 1, it is characterised in that: fatty in step S3
The addition total amount of enzyme, papain and cellulase is the 0.2% of Pleurotus eryngii powder.
6. a kind of method of high efficiency extraction Polysaccharide in Pleurotus eryngii according to claim 1, it is characterised in that: fatty in step S3
The mass ratio of enzyme, papain and cellulase is 1:3.8-4.2:1.8-2.2.
7. a kind of method of high efficiency extraction Polysaccharide in Pleurotus eryngii according to claim 1, it is characterised in that: fatty in step S3
The mass ratio of enzyme, papain and cellulase is 1:4:2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201811367187.3A CN109134691A (en) | 2018-11-16 | 2018-11-16 | A kind of method of high efficiency extraction Polysaccharide in Pleurotus eryngii |
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| CN201811367187.3A CN109134691A (en) | 2018-11-16 | 2018-11-16 | A kind of method of high efficiency extraction Polysaccharide in Pleurotus eryngii |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109907304A (en) * | 2019-03-21 | 2019-06-21 | 福建省农业科学院农业工程技术研究所 | A kind of preparation method of Polysaccharide in Pleurotus eryngii |
| CN110357982A (en) * | 2019-07-25 | 2019-10-22 | 芜湖职业技术学院 | A method of extracting Polysaccharide in Pleurotus eryngii |
| CN110372807A (en) * | 2019-07-11 | 2019-10-25 | 永富饶（天津）农业科技发展有限公司 | A kind of Polysaccharide in Pleurotus eryngii extraction process |
| CN113201077A (en) * | 2021-04-30 | 2021-08-03 | 南京财经大学 | Extraction method of pleurotus eryngii polysaccharide based on ultrasonic assistance |
-
2018
- 2018-11-16 CN CN201811367187.3A patent/CN109134691A/en not_active Withdrawn
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109907304A (en) * | 2019-03-21 | 2019-06-21 | 福建省农业科学院农业工程技术研究所 | A kind of preparation method of Polysaccharide in Pleurotus eryngii |
| CN110372807A (en) * | 2019-07-11 | 2019-10-25 | 永富饶（天津）农业科技发展有限公司 | A kind of Polysaccharide in Pleurotus eryngii extraction process |
| CN110357982A (en) * | 2019-07-25 | 2019-10-22 | 芜湖职业技术学院 | A method of extracting Polysaccharide in Pleurotus eryngii |
| CN113201077A (en) * | 2021-04-30 | 2021-08-03 | 南京财经大学 | Extraction method of pleurotus eryngii polysaccharide based on ultrasonic assistance |
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Application publication date: 20190104 |